SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_2804 FitnessBrowser__Cola:Echvi_2804 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5eynA Crystal structure of fructokinase from vibrio cholerae o395 in fructose, adp, beryllium trifluoride and calcium ion bound form
29% identity, 91% coverage: 9:277/295 of query aligns to 8:290/306 of 5eynA

query
sites
5eynA

G

G

E
x
D

M

A

L
x
V

W

V

D
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D

C

L

F

I

P

P

D

D

K

G

Q

Q

-

Q

-

H

-

Y

-

L

-

K

L

C

P

P

G
|
G

A
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A

P

P

M

A

N
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N

V

V

A

A

L

V

H

A

L

I

Q

A

H

R

L

L

G

S

I

G

T

R

T

S

T

A

F

F

I

F

S

G

K

R

I

V

G

G

S

N

D

D

S

P

L

F

G

G

A

R

D

F

L

M

L

Q

S

Q

F

T

V

L

Q

T

K

D

N

E

G

Q

L

V

N

D

G

C

D

Q

F

H

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L

Q

H

R

F

D

D

T

P

A

V

H

H

E

R

T

T

S

S

R

T

V
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V

V

V

D

D

N

L

S

D

D

E

K
x
H

E

G

N

E

I

R

K

S

Y
x
F

E

T

-
x
F

I

M

V

V

K

K

P

P

V

S

A

A

W

D

D

Q

F

F

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x
I

S

A

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L

A

A

A

N

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S

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Q

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N

T

-

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-

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T

A

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K

E

E

T

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G

T

G

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Y

K

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F

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I

P

N
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N

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R
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R

-

E

-

V

-

W

-

S

P

E

P

P

H

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E

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Q

K

A

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N

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K

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N

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E

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E

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L

E

Q

I

F

L

L

I

T

E

G

M

T

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A

S

L

I

D

-

K

-

N

-

E

-

E

E

K

E

A

G

L

L

S

Q

A

A

I

I

V

A

D

D

R

-

Y

F

E

Q

L

I

Q

P

L

L

V

V

C

V

M

V

T
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T

K

L

G
|
G

S
x
A

A

K

G
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G

A

A

I

L

I

V

Y

A

D

T

G

P

R

N

E

S

F

Q

Y

Q

R

I

H

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P

S

G

G

Y

K

Q
x
A

V
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V

D

K

V
x
P

E

I

D

D

T
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T

V
x
T

G
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G

S
x
A

G
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G

D
|
D

A

A

F
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F

L

V

S

G

G

G

F

L

-

L

-

Y

-

R

I

L

S

S

Q

V

Y

A

L

Q

K

D

G

W

N

H

H

N

P

Q

N

A

K

T

I

I

L

L

D

D

-

A

-

V

-

K

F

W

A

A

C
x
N

A

G

L

C

G
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G

A
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A

L

L

V

A

A

T

T

T

Q

Q

K

K

G

G

active site: G246 (= G239), A247 (≠ S240), G248 (= G241), D249 (= D242)
binding adenosine-5'-diphosphate: H91 (≠ K87), T217 (= T210), G219 (= G212), A220 (≠ S213), G222 (= G215), A238 (≠ Q231), V239 (= V232), P241 (≠ V234), T244 (= T237), G246 (= G239), A247 (≠ S240), G248 (= G241), F251 (= F244), N279 (≠ C266), G282 (= G269), A283 (= A270)
binding beryllium trifluoride ion: N157 (= N150), R159 (= R152), T245 (≠ V238), G246 (= G239), A247 (≠ S240), G248 (= G241), D249 (= D242)
binding beta-D-fructofuranose: D9 (≠ E10), V11 (≠ L12), D13 (= D14), G27 (= G23), G28 (= G24), A29 (= A25), N32 (= N28), V84 (= V80), F96 (≠ Y92), F98 (vs. gap), I127 (≠ G122), N157 (= N150), R159 (= R152), G246 (= G239), D249 (= D242)

5yggA Crystal structure of fructokinase double-mutant (t261c-h108c) from vibrio cholerae o395 in fructose, adp and potassium ion bound form (see paper)
28% identity, 91% coverage: 9:277/295 of query aligns to 12:294/310 of 5yggA

query
sites
5yggA

G

G

E
x
D

M

A

L
x
V

W

V

D
|
D

C

L

F

I

P

P

D

D

K

G

Q

Q

-

Q

-

H

-

Y

-

L

-

K

L

C

P

P

G
|
G

A
|
A

P

P

M

A

N
|
N

V

V

A

A

L

V

H

A

L

I

Q

A

H

R

L

L

G

S

I

G

T

R

T

S

T

A

F

F

I

F

S

G

K

R

I

V

G

G

S

N

D

D

S

P

L

F

G

G

A

R

D

F

L

M

L

Q

S

Q

F

T

V

L

Q

T

K

D

N

E

G

Q

L

V

N

D

G

C

D

Q

F

H

V

L

Q

H

R

F

D

D

T

P

A

V

H

H

E

R

T

T

S

S

R

T

V
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V

V

V

D

D

N

L

S

D

D

E

K

C

E

G

N

E

I

R

K

S

Y
x
F

E

T

-
x
F

I

M

V

V

K

K

P

P

V

S

A

A

W

D

D

Q

F

F

M

L

E

Q

W

L

N

S

T

D

A

I

I

P

Q

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E

F

K

Q

V

K

D

G

E

E

A

W

D

L

V

H

F

V

V

C

F

S

G
x
I

S

A

L

L

A

A

A

N

R

Q

S

P

S

S

Q

R

S

S

Q

S

N

T

-

F

-

A

T

A

L

I

F

A

R

Q

L

M

L

K

E

E

T

V

S

G

T

G

L

Y

K

V

V

S

L

F

D

D

I

P

N
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N

L

L

R
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R

-

E

-

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W

-

S

P

E

P

P

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E

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L

T

Q

K

A

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T

L

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A

L

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G

N

L

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A

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D

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K

I

F

N
x
S

E

E

D

E

E

E

L

L

E

Q

I

F

L

L

I

T

E

G

M

T

S

Q

A

S

L

I

D

-

K

-

N

-

E

-

E

E

K

E

A

G

L

L

S

Q

A

A

I

I

V

A

D

D

R

-

Y

F

E

Q

L

I

Q

P

L

L

V

V

C

V

M

V

T
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T

K

L

G
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G

S
x
A

A

K

G
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G

A

A

I

L

I

V

Y

A

D

T

G

P

R

N

E

S

F

Q

Y

Q

R

I

H

V

P

S

G

G

Y
x
K

Q
x
A

V
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V

D

K

V

P

E

I

D

D

T

C

V

T

G

G

S
x
A

G
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G

D
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D

A

A

F
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F

L

V

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G

G

F

L

-

L

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Y

-

R

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D

G

W

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H

H

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A

K

T

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I

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L

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D

-

A

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V

-

K

F

W

A

A

C
x
N

A

G

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G

A
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A

L

L

V

A

A

T

T

T

Q

Q

K

K

G

G

binding adenosine-5'-diphosphate: K188 (= K172), S190 (≠ N174), T221 (= T210), G223 (= G212), A224 (≠ S213), G226 (= G215), K241 (≠ Y230), A242 (≠ Q231), V243 (= V232), A251 (≠ S240), G252 (= G241), F255 (= F244), N283 (≠ C266), G286 (= G269), A287 (= A270)
binding beta-D-fructofuranose: D13 (≠ E10), V15 (≠ L12), D17 (= D14), G31 (= G23), G32 (= G24), A33 (= A25), N36 (= N28), V88 (= V80), F100 (≠ Y92), F102 (vs. gap), I131 (≠ G122), N161 (= N150), R163 (= R152), D253 (= D242)

3ih0A Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with amp-pnp
28% identity, 89% coverage: 22:283/295 of query aligns to 31:291/304 of 3ih0A

query
sites
3ih0A

P

P

G

G

A

A

P

P

M

A

N

N

V

V

A

A

L

V

H

G

L

V

Q

S

H

R

L

L

G

G

I

V

T

K

T

S

F

L

I

I

S

S

K

K

I

V

G

G

S

N

D

D

S

P

L

F

G

G

A

E

D

Y

L

L

L

I

S

E

F

E

V

L

Q

S

K

K

N

E

G

N

L

V

N

D

G

T

D

R

F

G

V

I

Q

V

R

K

D

D

T

E

A

K

H

K

E

H

T

T

S

G

R

I

V

V

V

F

V

V

D

-

N

Q

S

L

D

K

K

G

E

A

N

S

I

P

K

S

Y

F

E

L

I

L

V

Y

K

D

P

D

V

V

A

A

W

Y

D

F

F

N

M

M

E

T

W

L

N

N

T

D

A

I

I

N

Q

W

E

D

K

I

V

V

D

E

E

E

A

A

D

K

V

I

F

V

V

N

F

F

G

G

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S

L

V

A

I

A

L

R

A

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R

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S

S

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T

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M

R

K

L

V

L

I

E

K

T

K

-

I

-

K

-

G

S

S

T

S

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L

K

I

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A

L

F

D

D

I

V

N

N

L

L

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R

P

L

P

D

H

L

Y

W

S

R

-

G

-

Q

-

E

-

M

T

I

K

K

V

V

L

L

E

E

R

E

L

S

L

I

K

K

N

L

T

A

D

D

V

I

L

V

K
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K

I

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N

S

E

E

D

E

E

E

L

V

E

L

I

Y

L

L

I

E

E

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S

G

A

V

L

E

D

V

K

K

N

G

E

S

E

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K

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A

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S

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A

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I

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V

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D

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R

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Y

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E

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-

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M

L

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T

C

A

M

I

T
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T

K

L

G
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G

S
x
P

A

K

G
|
G

A
x
F

I

R

I

L

Y

I

D

K

G

N

R

E

E

T

F

V

Y

V

R

D

H

V

P

P

G
x
S

Y

Y

Q

N

V
|
V

D

N

V
x
P

E

L

D

D

T
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T

V

T

G
|
G

S
x
A

G
|
G

D
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D

A

A

F

F

L

M

S

A

G

A

F

L

I

L

S

V

Q

G

Y

I

L

L

K

K

G

L

N

K

H

G

P

L

N

D

K

L

I

L

L

K

D

L

F

G

A

K

C

F

A

A

-

N

L

L

G

V

A
|
A

L

A

V

L

A

S

T
|
T

Q

Q

K

K

G

R

G

G

-

A

-

W

-

S

T

T

P

P

R

R

Y

K

D

D

active site: G243 (= G239), A244 (≠ S240), G245 (= G241), D246 (= D242)
binding phosphoaminophosphonic acid-adenylate ester: K189 (= K172), T214 (= T210), G216 (= G212), P217 (≠ S213), G219 (= G215), F220 (≠ A216), S233 (≠ G229), V236 (= V232), P238 (≠ V234), T241 (= T237), A244 (≠ S240), G245 (= G241), A275 (= A270), T279 (= T274)

3gbuA Crystal structure of an uncharacterized sugar kinase ph1459 from pyrococcus horikoshii in complex with atp
28% identity, 89% coverage: 22:283/295 of query aligns to 30:290/302 of 3gbuA

query
sites
3gbuA

P

P

G

G

A

A

P

P

M

A

N

N

V

V

A

A

L

V

H

G

L

V

Q

S

H

R

L

L

G

G

I

V

T

K

T

S

F

L

I

I

S

S

K

K

I

V

G

G

S

N

D

D

S

P

L

F

G

G

A

E

D

Y

L

L

L

I

S

E

F

E

V

L

Q

S

K

K

N

E

G

N

L

V

N

D

G

T

D

R

F

G

V

I

Q

V

R

K

D

D

T

E

A

K

H

K

E

H

T

T

S

G

R

I

V

V

V

F

V

V

D

-

N

Q

S

L

D

K

K

G

E

A

N

S

I

P

K

S

Y

F

E

L

I

L

V

Y

K

D

P

D

V

V

A

A

W

Y

D

F

F

N

M

M

E

T

W

L

N

N

T

D

A

I

I

N

Q

W

E

D

K

I

V

V

D

E

E

E

A

A

D

K

V

I

F

V

V

N

F

F

G

G

S

S

L

V

A

I

A

L

R

A

S

R

S

N

Q

P

S

S

Q

R

N

E

T

T

L

V

F

M

R

K

L

V

L

I

E

K

T

K

-

I

-

K

-

G

S

S

T

S

L

L

K

I

V

A

L

F

D

D

I

V

N

N

L

L

R

R

P

L

P

D

H

L

Y

W

S

R

-

G

-

Q

-

E

-

M

T

I

K

K

V

V

L

L

E

E

R

E

L

S

L

I

K

K

N

L

T

A

D

D

V

I

L

V

K
|
K

I

A

N

S

E

E

D

E

E

E

L

V

E

L

I

Y

L

L

I

E

E

N

M

Q

S

G

A

V

L

E

D

V

K

K

N

G

E

S

E

-

K

-

A

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L

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S

-

A

-

I

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V

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D

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R

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Y

-

E

-

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-

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M

L

L

V

T

C

A

M

I

T
|
T

K

L

G
|
G

S
x
P

A

K

G
|
G

A
x
F

I

R

I

L

Y

I

D

K

G

N

R

E

E

T

F

V

Y

V

R

D

H

V

P

P

G
x
S

Y

Y

Q

N

V
|
V

D

N

V
x
P

E

L

D

D

T
|
T

V

T

G
|
G

S
x
A

G
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G

D
|
D

A

A

F

F

L

M

S

A

G

A

F

L

I

L

S

V

Q

G

Y

I

L

L

K

K

G

L

N

K

H

G

P

L

N

D

K

L

I

L

L

K

D

L

F

G

A

K

C

F

A

A

-

N

L

L

G

V

A
|
A

L

A

V

L

A

S

T
|
T

Q

Q

K

K

G

R

G

G

-

A

-

W

-

S

T

T

P

P

R

R

Y

K

D

D

active site: G242 (= G239), A243 (≠ S240), G244 (= G241), D245 (= D242)
binding adenosine-5'-triphosphate: K188 (= K172), T213 (= T210), G215 (= G212), P216 (≠ S213), G218 (= G215), F219 (≠ A216), S232 (≠ G229), V235 (= V232), P237 (≠ V234), T240 (= T237), A243 (≠ S240), G244 (= G241), A274 (= A270), T278 (= T274)

Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
28% identity, 94% coverage: 1:277/295 of query aligns to 4:292/319 of Q8ZKR2

query
sites
Q8ZKR2

M

M

N

N

K

K

K

V

A

W

V

V

I

I

F

-

G

G

E

D

M

A

L

S

W

V

D
|
D

C

L

F

V

P

P

D

E

K

K

Q

Q

-

N

-

S

-

Y

-

L

-

K

L

C

P

P

G

G

G
|
G

A

A

P

S

M

A

N

N

V

V

A

G

L

V

H

C

L

V

Q

A

H

R

L

L

G

G

I

G

T

E

T

C

T

G

F

F

I

I

S

G

K

C

I

L

G

G

S

D

D

D

S

D

L

A

G

G

A

R

D

F

L

L

L

R

S

Q

F

V

V

F

Q

Q

K

D

N

N

G

G

L

V

N

D

G

V

D

T

F

F

V

L

Q

R

R

L

D

D

T

-

A

A

H

D

E

L

T

T

S

S

R

A

V

V

V

L

V

I

D

V

N

N

-

L

-

T

S

A

D

D

K

G

E

E

N

R

I

S

K

F

Y

T

E
x
Y

I

L

V

V

K

H

P

P

V

G

A

A

W

D

D

T

F

Y

M

V

E

-

W

-

N

S

T

P

A

Q

I

D

Q

L

E

P

K

P

V

F

D

R

E

Q

A

Y

D

E

V

W

F

F

V

Y

F

F

G

S

S

S

L

I

A

G

A

L

R

T

S

D

S

R

Q

P

S

A

Q

R

N

E

T

A

L

C

F

L

-

E

-

G

-

A

-

R

R

R

L

M

L

R

E

E

T

A

S

G

T

G

L

Y

K

V

V

L

L

F

D

D

I

V

N

N

L

L

R
|
R

P

S

P

K

H

M

Y

W

S

G

T

N

-

T

-

D

-

E

-

I

-

P

K

E

V

L

L

I

E

A

R

R

L

S

L
x
A

K
x
A

N

L

T
x
A

D

S

V

I

L

C

K

K

I

V

N

S

E

A

D

D

E

E

L

L

E

-

I

-

L

-

I

C

E

Q

M

L

S

S

A

G

L

A

D

S

K

H

N

W

E

Q

E

D

K

A

A

-

L

-

S

-

A

-

I

-

V

-

D

-

R

R

Y

Y

E

Y

L

L

Q

R

-

D

L

L

V
x
G

C

C

M

D

T

T

K

T

-

I

-

S

-

L

G

G

S

A

A

D

G

G

A

A

I

L

Y

I

D

T

G

A

R

E

E

G

F

E

Y

F

R

H

H

F

P

P

G

A

Y

P

Q

R

V

V

D

D

V

V

E

V

D
|
D

T

T

V
x
T

G

G

S

A

G

G

D
|
D

A

A

F

F

L

V

S

G

G

G

F

L

I

L

S

F

Q

T

Y

L

L

S

K

R

G

A

N

N

H

C

P

W

N

D

K

H

I

A

L

L

-

L

-

A

-

E

-

A

-

I

D

S

F

N

A

A

C

N

A

A

L

C

G

G

A

A

L

M

V
x
A

A

V

T

T

Q
x
A

K

K

G
|
G

D16 (= D14) binding
G31 (= G24) binding
Y101 (≠ E93) binding
R162 (= R152) binding
A180 (≠ L165) binding
A181 (≠ K166) binding
A183 (≠ T168) binding
G213 (≠ V207) binding
D246 (= D236) binding
T248 (≠ V238) binding
D252 (= D242) binding
A287 (≠ V272) binding
A290 (≠ Q275) binding
G292 (= G277) binding

2dcnA Crystal structure of 2-keto-3-deoxygluconate kinase from sulfolobus tokodaii complexed with 2-keto-6-phosphogluconate (alpha-furanose form)
27% identity, 86% coverage: 23:277/295 of query aligns to 32:290/308 of 2dcnA

query
sites
2dcnA

G
x
A

G
|
G

A
x
S

P

E

M

A

N
|
N

V

Y

A

C

L

V

H

A

L

F

Q

I

H

K

L

Q

G

G

I

N

T

E

T

C

T

G

F

I

I

I

S

A

K

K

I

V

G

G

S

D

D

D

S

E

L

F

G

G

A

Y

D

N

L

A

L

I

S

E

F

W

V

L

Q

R

K

G

N

Q

G

G

L

V

N

D

G

V

D

S

F

H

V

M

Q

K

R

I

D

D

T

P

A

S

H

A

E

P

T

T

S

G

R

I

V
x
F

V

F

V

I

D

Q

N

R

S

H

D

Y

K

P

E

V

N

P

I

L

K

K

Y

S

E

E

I

S

V

I

-
x
Y

-

Y

-
x
R

K

K

P

G

V

S

A

A

W

G

D

S

F

K

M

L

E

S

W

P

N

E

T

D

A

V

I

D

Q

E

E

E

K

Y

V

V

D

K

E

S

A

A

D

D

-

L

V

V

F

H

V

S

F

S

G

G

S
x
I

L

T

A

L

A

A

R

I

S

S

Q

T

S

A

Q

K

N

E

T

A

L

V

F

Y

R

K

L

A

L

F

E

E

T

I

S

A

T

S

L

N

K

R

V

S

L

F

D

D

I

T

N

N

L

I

R
|
R

P

L

P

K

H

L

Y

W

S

S

T

A

K

E

V

E

L

A

E

K

R

R

-

E

L

I

L

L

K

K

-

L

-

S

-

K

-

F

N

H

T

L

D

K

V

F

L

L

K

I

I

T

N
x
D

E

T

D

D

E

D

L

S

E

K

I

I

L

I

I

L

E

G

M

E

S

S

A

-

L

-

D

-

K

-

N

D

E

P

E

D

K

K

A

A

L

A

S

K

A

A

I

F

V

S

D

D

R

Y

Y

A

E

E

L

I

Q

-

L

-

V

I

C

V

M

M

T
x
K

K
x
L

G
|
G

S
x
P

A

K

G
|
G

A

A

I

I

I

V

Y

Y

-

Y

D

D

G

G

R

K

E

K

F

Y

Y

Y

R

S

H

S

P

-

G

G

Y
|
Y

Q

Q

V

V

D

P

V
|
V

E

E

D

D

T
x
V

V
x
T

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F
x
L

L

G

S

G

G

T

F

F

I

L

S

S

Q

L

Y

Y

L

Y

K

K

G

G

N

F

H

E

P

M

N

E

K

K

I

A

L

L

D

D

F

Y

A

A

C
x
I

A

V

L

A

G
x
S

A

T

L

L

V

N

A
x
V

T

M

Q

I

K

R

G

G

active site: G252 (= G239), A253 (≠ S240), G254 (= G241), D255 (= D242)
binding adenosine-5'-diphosphate: D193 (≠ N174), K223 (≠ T210), L224 (≠ K211), G225 (= G212), P226 (≠ S213), G228 (= G215), Y243 (= Y230), V247 (= V234), V250 (≠ T237), A253 (≠ S240), G254 (= G241), L257 (≠ F244), I279 (≠ C266), S282 (≠ G269), V286 (≠ A273)
binding 6-O-phosphono-beta-D-psicofuranosonic acid: A32 (≠ G23), G33 (= G24), S34 (≠ A25), N37 (= N28), F89 (≠ V80), Y105 (vs. gap), R107 (vs. gap), I136 (≠ S123), R165 (= R152), T251 (≠ V238), G252 (= G239), A253 (≠ S240), G254 (= G241), D255 (= D242)

Sites not aligning to the query:

binding 6-O-phosphono-beta-D-psicofuranosonic acid: 10, 291

1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
27% identity, 93% coverage: 4:277/295 of query aligns to 2:281/297 of 1tz6A

query
sites
1tz6A

K

K

A

V

V

W

I

V

F

I

G

G

E
x
D

M

A

L
x
S

W

V

D
|
D

C

L

F

V

P

P

D

E

K

K

Q

Q

-

N

-

S

-

Y

-

L

-

K

L
x
C

P

P

G
|
G

A
|
A

P

S

M

A

N
|
N

V

V

A

G

L

V

H

C

L

V

Q

A

H

R

L

L

G

G

I

G

T

E

T

C

T

G

F

F

I

I

S

G

K

C

I

L

G

G

S

D

D

D

S

D

L

A

G

G

A

R

D

F

L

L

L

R

S

Q

F

V

V

F

Q

Q

K

D

N

N

G

G

L

V

N

D

G

V

D

T

F

F

V

L

Q

R

R

L

D

D

T

-

A

A

H

D

E

L

T

T

S

S

R

A

V

V

V
x
L

V

I

D

V

N

N

S

S

D

-

K

-

E

-

N

-

I
x
F

K

T

Y
|
Y

E

-

I

L

V

V

K

H

P

P

V

G

A

A

W

D

D

T

F

Y

M

V

E

-

W

-

N

S

T

P

A

Q

I

D

Q

L

E

P

K

P

V

F

D

R

E

Q

A

Y

D

E

V

W

F

F

V

Y

F

F

G

S

S

S

L

I

A

G

A

L

R

T

S

D

S

R

Q

P

S

A

Q

R

N

E

T

A

L

C

F

L

-

E

-

G

-

A

-

R

R

R

L

M

L

R

E

E

T

A

S

G

T

G

L

Y

K

V

V

L

L

F

D
|
D

I

V

N
|
N

L

L

R
|
R

P

S

P

K

H
x
M

Y

W

S

G

T

N

-

T

-

D

-

E

-

I

-

P

K

E

V

L

L

I

E

A

R

R

L

S

L

A

K

A

N

L

T

A

D

S

V

I

L

C

K
|
K

I

V

N
x
S

E

A

D

D

E
|
E

L

-

E

-

I

-

L

L

I

C

E

Q

M

L

S

S

A

G

L

A

D

S

K

H

N

W

E

Q

E

D

K

A

A

-

L

-

S

-

A

-

I

-

V

-

D

-

R

R

Y

Y

E

Y

L

L

Q

R

-

D

L

L

V

G

C

C

M

D

T

T

K

T

-

I

-
x
S

-

L

G
|
G

S
x
A

A

D

G
|
G

A

A

I

L

Y

I

D

T

G

A

R

E

E

G

F

E

Y

F

R

H

H

F

P

P

G
x
A

Y

P

Q

R

V
|
V

D

D

V
|
V

E

V

D

D

T

T

V
x
T

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F
|
F

L

V

S

G

G

G

F

L

I

L

S

F

Q

T

Y

L

L

S

K

R

G

A

N

N

H

C

P

W

N

D

K

H

I

A

L

L

-

L

-

A

-

E

-

A

-

I

D

S

F

N

A

A

C
x
N

A

A

L

C

G
|
G

A
|
A

L

M

V

A

A
x
V

T

T

Q

A

K

K

G

G

active site: C24 (≠ L21), F88 (≠ I90), G238 (= G239), A239 (≠ S240), G240 (= G241), D241 (= D242)
binding phosphomethylphosphonic acid adenylate ester: D147 (= D148), N149 (= N150), K176 (= K172), S178 (≠ N174), E181 (= E177), S209 (vs. gap), G211 (= G212), A212 (≠ S213), G214 (= G215), A228 (≠ G229), V231 (= V232), V233 (= V234), A239 (≠ S240), G240 (= G241), F243 (= F244), N270 (≠ C266), G273 (= G269), A274 (= A270), V277 (≠ A273)
binding 5-aminoimidazole ribonucleoside: D8 (≠ E10), S10 (≠ L12), D12 (= D14), G26 (= G23), G27 (= G24), A28 (= A25), N31 (= N28), L83 (≠ V81), F88 (≠ I90), Y90 (= Y92), R151 (= R152), M154 (≠ H155), T237 (≠ V238), D241 (= D242)

Q53W83 2-dehydro-3-deoxygluconokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; EC 2.7.1.45 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
28% identity, 86% coverage: 23:277/295 of query aligns to 33:286/309 of Q53W83

query
sites
Q53W83

G

G

G
|
G

A
|
A

P
x
E

M
x
V

N
|
N

V

V

A

A

L

V

H

A

L

L

Q

A

H

R

L

L

G

G

I

V

T

K

T

V

T

G

F

F

I

V

S

G

K

R

I

V

G

G

S

E

D

D

S

E

L

L

G

G

A

A

-

M

-

V

-

E

-

R

-

L

-

R

-

A

-

E

-

G

-

V

D

D

L

L

L

T

S

H

F

F

V

R

Q

R

K

A

N

P

G

G

L

F

N

T

G

G

D

L

F

Y

V

L

Q

R

R

E

D

Y

T

L

A

P

H

L

E

G

T

Q

S

G

R

R

V

V

V

F

V
x
Y

D
x
Y

N
x
R

S

K

D

G

K

S

E

A

N

G

I

-

K

-

Y

-

E

S

I

A

V

L

K

A

P

P

V

G

A

A

W

F

D

D

-

P

-

D

-

Y

-

L

-

E

-

G

-

V

-

R

F

F

M

L

E

H

W

L

N

S

T

G

A

I

I

T

Q

P

E

A

K

L

V

S

D

P

E

E

A

A

D

R

V

A

F

F

V

-

F

-

G

-

S

S

L

L

A

W

A

A

R

M

S

E

Q

A

S

K

Q

R

N

-

T

-

L

-

F

-

R

R

L

G

L

V

E

R

T

V

S

S

T

-

L

-

K

-

V

-

L

L

D

D

I

V

N

N

L

Y

R
|
R

-

Q

-

T

-

L

-

W

P

S

P

P

H

E

Y

E

S

A

T

R

K

G

V

F

L

L

E

E

R

R

L

A

L

L

K

P

N

G

T

V

D

D

V

L

L

L

K

F

I

L

N
x
S

E

E

D

E

E

E

L

A

E

E

I

L

L

L

I

-

E

-

M

-

S

-

A

-

L

F

D

G

K

R

N

V

E

E

-

A

-

L

K

R

A

A

L

L

S

S

A

A

I

-

V

-

D

-

R

-

Y

-

E

-

L

-

Q

P

L

E

V

V

C

V

M

L

T
x
K

K
x
R

G
|
G

S
x
A

A
x
K

G
|
G

A
|
A

I

W

I

A

Y

F

-

V

D

D

G

G

R

R

E

R

F

V

Y

-

R

E

H

G

P

S

G

A

Y

F

Q

A

V

V

D

E

V

A

E

V

D

D

T

P

V

V

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F

F

L

A

S

A

G

G

F

Y

I

L

S

A

Q

G

Y

A

L

V

K

W

G

G

N

L

H

P

P

V

N

E

K

E

I

R

L

L

D

R

F

L

A

A

C
x
N

A

L

L

L

G

G

A

A

L

S

V

V

A

A

T

A

Q

S

K

R

G

G

GAEVN 34:38 (≠ GAPMN 24:28) binding
YYR 103:105 (≠ VDN 82:84) binding
R167 (= R152) binding
S193 (≠ N174) binding
219:225 (vs. 210:216, 43% identical) binding
GAGD 248:251 (≠ GSGD 239:242) binding
D251 (= D242) binding
N275 (≠ C266) binding

Sites not aligning to the query:

287 binding

1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
27% identity, 93% coverage: 4:277/295 of query aligns to 2:281/299 of 1tz3A

query
sites
1tz3A

K

K

A

V

V

W

I

V

F

I

G

G

E
x
D

M

A

L
x
S

W

V

D
|
D

C

L

F

V

P

P

D

E

K

K

Q

Q

-

N

-

S

-

Y

-

L

-

K

L
x
C

P

P

G
|
G

A
|
A

P

S

M

A

N

N

V

V

A

G

L

V

H

C

L

V

Q

A

H

R

L

L

G

G

I

G

T

E

T

C

T

G

F

F

I

I

S

G

K

C

I

L

G

G

S

D

D

D

S

D

L

A

G

G

A

R

D

F

L

L

L

R

S

Q

F

V

V

F

Q

Q

K

D

N

N

G

G

L

V

N

D

G

V

D

T

F

F

V

L

Q

R

R

L

D

D

T

-

A

A

H

D

E

L

T

T

S

S

R

A

V

V

V
x
L

V

I

D

V

N

N

S

S

D

-

K

-

E

-

N

-

I
x
F

K

T

Y
|
Y

E

-

I

L

V

V

K

H

P

P

V

G

A

A

W

D

D

T

F

Y

M

V

E

-

W

-

N

S

T

P

A

Q

I

D

Q

L

E

P

K

P

V

F

D

R

E

Q

A

Y

D

E

V

W

F

F

V

Y

F

F

G

S

S

S

L

I

A

G

A

L

R

T

S

D

S

R

Q

P

S

A

Q

R

N

E

T

A

L

C

F

L

-

E

-

G

-

A

-

R

R

R

L

M

L

R

E

E

T

A

S

G

T

G

L

Y

K

V

V

L

L

F

D

D

I

V

N

N

L

L

R
|
R

P

S

P

K

H
x
M

Y

W

S

G

T

N

-

T

-

D

-

E

-

I

-

P

K

E

V

L

L

I

E

A

R

R

L

S

L

A

K

A

N

L

T

A

D

S

V

I

L

C

K

K

I

V

N

S

E

A

D

D

E

E

L

-

E

-

I

-

L

L

I

C

E

Q

M

L

S

S

A

G

L

A

D

S

K

H

N

W

E

Q

E

D

K

A

A

-

L

-

S

-

A

-

I

-

V

-

D

-

R

R

Y

Y

E

Y

L

L

Q

R

-

D

L

L

V

G

C

C

M

D

T

T

K

T

-

I

-

S

-

L

G

G

S

A

A

D

G

G

A

A

I

L

Y

I

D

T

G

A

R

E

E

G

F

E

Y

F

R

H

H

F

P

P

G

A

Y

P

Q

R

V

V

D

D

V

V

E

V

D

D

T

T

V

T

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F

F

L

V

S

G

G

G

F

L

I

L

S

F

Q

T

Y

L

L

S

K

R

G

A

N

N

H

C

P

W

N

D

K

H

I

A

L

L

-

L

-

A

-

E

-

A

-

I

D

S

F

N

A

A

C

N

A

A

L

C

G

G

A

A

L

M

V

A

A

V

T

T

Q

A

K

K

G

G

active site: C24 (≠ L21), F88 (≠ I90), G238 (= G239), A239 (≠ S240), G240 (= G241), D241 (= D242)
binding 5-aminoimidazole ribonucleoside: D8 (≠ E10), S10 (≠ L12), D12 (= D14), G26 (= G23), G27 (= G24), A28 (= A25), L83 (≠ V81), F88 (≠ I90), Y90 (= Y92), R151 (= R152), M154 (≠ H155), D241 (= D242)

1v1bA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound atp (see paper)
28% identity, 86% coverage: 23:277/295 of query aligns to 33:286/300 of 1v1bA

query
sites
1v1bA

G

G

A

A

P

E

M

V

N

N

V

V

A

A

L

V

H

A

L

L

Q

A

H

R

L

L

G

G

I

V

T

K

T

V

T

G

F

F

I

V

S

G

K

R

I

V

G

G

S

E

D

D

S

E

L

L

G

G

A

A

-

M

-

V

-

E

-

R

-

L

-

R

-

A

-

E

-

G

-

V

D

D

L

L

L

T

S

H

F

F

V

R

Q

R

K

A

N

P

G

G

L

F

N

T

G

G

D

L

F

Y

V

L

Q

R

R

E

D

Y

T

L

A

P

H

L

E

G

T

Q

S

G

R

R

V

V

V

F

V

Y

D

Y

N

R

S

K

D

G

K

S

E

A

N

G

I

-

K

-

Y

-

E

S

I

A

V

L

K

A

P

P

V

G

A

A

W

F

D

D

-

P

-

D

-

Y

-

L

-

E

-

G

-

V

-

R

F

F

M

L

E

H

W

L

N

S

T

G

A

I

I

T

Q

P

E

A

K

L

V

S

D

P

E

E

A

A

D

R

V

A

F

F

V

-

F

-

G

-

S

S

L

L

A

W

A

A

R

M

S

E

Q

A

S

K

Q

R

N

-

T

-

L

-

F

-

R

R

L

G

L

V

E

R

T

V

S

S

T

-

L

-

K

-

V

-

L

L

D

D

I

V

N
|
N

L

Y

R

R

-

Q

-

T

-

L

-

W

P

S

P

P

H

E

Y

E

S

A

T

R

K

G

V

F

L

L

E

E

R

R

L

A

L

L

K

P

N

G

T

V

D

D

V

L

L

L

K

F

I

L

N
x
S

E

E

D

E

E

E

L

A

E

E

I

L

L

L

I

-

E

-

M

-

S

-

A

-

L

F

D

G

K

R

N

V

E

E

-

A

-

L

K

R

A

A

L

L

S

S

A

A

I

-

V

-

D

-

R

-

Y

-

E

-

L

-

Q

P

L

E

V

V

C

V

M

L

T
x
K

K

R

G
|
G

S

A

A

K

G
|
G

A

A

I

W

I

A

Y

F

-

V

D

D

G

G

R

R

E

R

F

V

Y

-

R

E

H

G

P

S

G
x
A

Y
x
F

Q

A

V
|
V

D

E

V

A

E

V

D

D

T
x
P

V
|
V

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F
|
F

L

A

S

A

G

G

F

Y

I

L

S

A

Q

G

Y

A

L

V

K

W

G

G

N

L

H

P

P

V

N

E

K

E

I

R

L

L

D

R

F

L

A

A

C
x
N

A

L

L

L

G
|
G

A
|
A

L

S

V

V

A
|
A

T

A

Q

S

K

R

G

G

active site: G248 (= G239), A249 (≠ S240), G250 (= G241), D251 (= D242)
binding adenosine-5'-triphosphate: N165 (= N150), S193 (≠ N174), K219 (≠ T210), G221 (= G212), G224 (= G215), A238 (≠ G229), F239 (≠ Y230), V241 (= V232), P246 (≠ T237), V247 (= V238), G248 (= G239), A249 (≠ S240), G250 (= G241), D251 (= D242), F253 (= F244), N275 (≠ C266), G278 (= G269), A279 (= A270), A282 (= A273)

1v1aA 2-keto-3-deoxygluconate kinase from thermus thermophilus with bound 2- keto-3-deoxygluconate and adp (see paper)
28% identity, 86% coverage: 23:277/295 of query aligns to 33:286/301 of 1v1aA

query
sites
1v1aA

G
|
G

A
|
A

P

E

M

V

N
|
N

V

V

A

A

L

V

H

A

L

L

Q

A

H

R

L

L

G

G

I

V

T

K

T

V

T

G

F

F

I

V

S

G

K

R

I

V

G

G

S

E

D

D

S

E

L

L

G

G

A

A

-

M

-

V

-

E

-

R

-

L

-

R

-

A

-

E

-

G

-

V

D

D

L

L

L

T

S

H

F

F

V

R

Q

R

K

A

N

P

G

G

L

F

N

T

G

G

D

L

F
x
Y

V

L

Q

R

R

E

D

Y

T

L

A

P

H

L

E

G

T

Q

S

G

R

R

V

V

V

F

V
x
Y

D

Y

N
x
R

S

K

D

G

K

S

E

A

N

G

I

-

K

-

Y

-

E

S

I

A

V

L

K

A

P

P

V

G

A

A

W

F

D

D

-

P

-

D

-

Y

-

L

-

E

-

G

-

V

-

R

F

F

M

L

E

H

W

L

N

S

T

G

A
x
I

I

T

Q

P

E

A

K

L

V

S

D

P

E

E

A

A

D

R

V

A

F

F

V

-

F

-

G

-

S

S

L

L

A

W

A

A

R

M

S

E

Q

A

S

K

Q

R

N

-

T

-

L

-

F

-

R

R

L

G

L

V

E

R

T

V

S

S

T

-

L

-

K

-

V

-

L

L

D

D

I

V

N

N

L

Y

R
|
R

-

Q

-

T

-

L

-

W

P

S

P

P

H

E

Y

E

S

A

T

R

K

G

V

F

L

L

E

E

R

R

L

A

L

L

K

P

N

G

T

V

D

D

V

L

L

L

K

F

I

L

N

S

E

E

D

E

E

E

L

A

E

E

I

L

L

L

I

-

E

-

M

-

S

-

A

-

L

F

D

G

K

R

N

V

E

E

-

A

-

L

K

R

A

A

L

L

S

S

A

A

I

-

V

-

D

-

R

-

Y

-

E

-

L

-

Q

P

L

E

V

V

C

V

M

L

T
x
K

K

R

G
|
G

S
x
A

A

K

G

G

A

A

I

W

I

A

Y

F

-

V

D

D

G

G

R

R

E

R

F

V

Y

-

R

E

H

G

P

S

G

A

Y
x
F

Q

A

V
|
V

D

E

V
x
A

E

V

D

D

T

P

V

V

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F
|
F

L

A

S

A

G

G

F

Y

I

L

S

A

Q

G

Y

A

L

V

K

W

G

G

N

L

H

P

P

V

N

E

K

E

I

R

L

L

D

R

F

L

A

A

C
x
N

A

L

L

L

G
|
G

A
|
A

L

S

V

V

A
|
A

T

A

Q

S

K

R

G

G

active site: G248 (= G239), A249 (≠ S240), G250 (= G241), D251 (= D242)
binding adenosine-5'-diphosphate: K219 (≠ T210), G221 (= G212), A222 (≠ S213), F239 (≠ Y230), V241 (= V232), A243 (≠ V234), A249 (≠ S240), G250 (= G241), F253 (= F244), N275 (≠ C266), G278 (= G269), A279 (= A270), A282 (= A273)
binding 2-keto-3-deoxygluconate: G33 (= G23), G34 (= G24), A35 (= A25), N38 (= N28), Y89 (≠ F68), Y103 (≠ V82), R105 (≠ N84), I134 (≠ A108), R167 (= R152), G248 (= G239), D251 (= D242)

Sites not aligning to the query:

binding 2-keto-3-deoxygluconate: 11, 287

Q97U29 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase; 2-dehydro-3-deoxyglucono/galactono-kinase; 2-keto-3-deoxy-galactonokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; KDGal kinase; EC 2.7.1.178 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
25% identity, 86% coverage: 23:277/295 of query aligns to 33:293/313 of Q97U29

query
sites
Q97U29

G

A

G
|
G

A
x
S

P
x
E

M
x
L

N
|
N

V

F

A

C

L

I

H

A

L

V

Q

V

H

R

L

N

G

H

I

L

T

S

T

C

T

S

F

L

I

I

S

A

K

R

I

V

G

G

S

N

D

D

S

E

L

F

G

G

A

K

D

N

L

I

S

E

F

Y

V

S

Q

R

K

A

N

Q

G

G

L

I

N

D

G

T

D

S

F

H

V

I

Q

K

R

V

D

D

T

N

A

E

H

S

E

F

T

T

S

G

R

I

V
x
Y

V

F

V

I

D

Q

N

R

S

G

D

Y

K

P

E

I

N

P

I

M

K

K

Y

S

E

E

I

L

V

V

-
x
Y

-
x
R

K

K

P

G

V

S

A

A

W

G

D

S

F

R

M

L

E

S

W

P

N

E

T

D

A

I

I

N

Q

E

E

N

K

Y

V

V

D

R

E

N

A

S

D

R

-

L

V

V

F

H

V

S

F

T

G

G

S

I

L

T

A

L

A

A

R

I

S

S

S

D

Q

N

S

A

Q

K

N

E

T

A

L

V

F

I

R

K

L

A

L

F

E

E

T

L

S

A

T

K

L

S

K

R

V

S

L

L

D

D

I

T

N
|
N

L
x
I

R
|
R

P

P

P

K

H

L

Y

W

S

S

T

S

-

L

-

E

-

K

-

A

-

K

K

E

V

T

L

I

E

L

R

S

L

I

L

L

K

K

N

K

T

Y

D

D

-

I

-

E

V

V

L

L

K

I

I

T

N

D

E

P

D

D

E

D

L

T

E

K

I

I

L

L

I

L

E

D

M

V

S

T

A

D

L

P

D

D

K

E

-

A

-

Y

N

R

E

K

E

Y

K

K

A

E

L

L

S

G

A

V

I

K

V

V

D

L

R

L

Y

Y

E
x
K

L
|
L

Q

-

L

-

V

-

C

-

M

-

T

-

K

-

G
|
G

S
|
S

A
x
K

G
|
G

A

A

I

I

I

A

Y

Y

D

K

G

D

R

N

E

V

F

K

Y

A

R

F

H

K

P

D

G

A

Y

Y

Q

K

V

V

D

P

V

V

E

E

D

D

T

P

V

T

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F

M

L

A

S

G

G

T

F

F

I

V

S

S

Q

L

Y

Y

L

L

K

Q

G

G

N

K

H

D

P

I

N

E

K

Y

I

S

L

L

D

A

F

H

A

G

C

I

A

A

L

A

G

S

A

T

L

L

V

V

A

I

T

T

Q

V

K

R

G

G

GSELN 34:38 (≠ GAPMN 24:28) binding
Y90 (≠ V80) binding
YYR 106:108 (vs. gap) binding
NIR 164:166 (≠ NLR 150:152) binding
R166 (= R152) binding
KL-------GSKG 226:231 (≠ ELQLVCMTKGSAG 203:215) binding
GAGD 255:258 (≠ GSGD 239:242) binding
D258 (= D242) binding

Sites not aligning to the query:

294 binding

2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
25% identity, 86% coverage: 23:277/295 of query aligns to 32:292/311 of 2varA

query
sites
2varA

G
x
A

G
|
G

A
x
S

P

E

M

L

N
|
N

V

F

A

C

L

I

H

A

L

V

Q

V

H

R

L

N

G

H

I

L

T

S

T

C

T

S

F

L

I

I

S

A

K

R

I

V

G

G

S

N

D

D

S

E

L

F

G

G

A

K

D

N

L

I

S

E

F

Y

V

S

Q

R

K

A

N

Q

G

G

L

I

N

D

G

T

D

S

F

H

V

I

Q

K

R

V

D

D

T

N

A

E

H

S

E

F

T

T

S

G

R

I

V
x
Y

V

F

V

I

D

Q

N

R

S

G

D

Y

K

P

E

I

N

P

I

M

K

K

Y

S

E

E

I
x
L

V

V

-
x
Y

-

Y

-
x
R

K

K

P

G

V

S

A

A

W

G

D

S

F

R

M

L

E

S

W

P

N

E

T

D

A

I

I

N

Q

E

E

N

K

Y

V

V

D

R

E

N

A

S

D

R

-

L

V

V

F

H

V

S

F

T

G

G

S
x
I

L

T

A

L

A

A

R

I

S

S

S

D

Q

N

S

A

Q

K

N

E

T

A

L

V

F

I

R

K

L

A

L

F

E

E

T

L

S

A

T

K

L

S

K

R

V

S

L

L

D

D

I

T

N
|
N

L

I

R
|
R

P

P

P

K

H

L

Y

W

S

S

T

S

-

L

-

E

K

K

V

A

L

K

E

E

R

T

L

I

L

L

K

S

-

I

-

L

-

K

-

Y

N

D

T

I

D

E

V

V

L

L

K

I

I

T

N

D

E

P

D

D

E

D

L

T

E

K

I

I

L

L

I

L

E

D

M

V

S

T

A

D

L

P

D

D

K

E

-

A

-

Y

N

R

E

K

E

Y

K

K

A

E

L

L

S

G

A

V

I

K

V

V

D

L

R

L

Y

Y

E
x
K

L

L

Q

-

L

-

V

-

C

-

M

-

T

-

K

-

G
|
G

S
|
S

A

K

G
|
G

A
|
A

I

I

I

A

Y

Y

D

K

G

D

R

N

E

V

F

K

Y

A

R

F

H

K

P

D

G
x
A

Y
|
Y

Q

K

V

V

D

P

V
|
V

E

E

D

D

T

P

V
x
T

G
|
G

S
x
A

G
|
G

D
|
D

A

A

F
x
M

L

A

S

G

G

T

F

F

I

V

S

S

Q

L

Y

Y

L

L

K

Q

G

G

N

K

H

D

P

I

N

E

K

Y

I

S

L

L

D

A

F

H

A

G

C
x
I

A

A

L

A

G
x
S

A

T

L

L

V

V

A
x
I

T

T

Q

V

K

R

G

G

active site: G254 (= G239), A255 (≠ S240), G256 (= G241), D257 (= D242)
binding adenosine monophosphate: K225 (≠ E203), G227 (= G212), S228 (= S213), G230 (= G215), A231 (= A216), A244 (≠ G229), Y245 (= Y230), V249 (= V234), A255 (≠ S240), G256 (= G241), M259 (≠ F244), I281 (≠ C266), S284 (≠ G269), I288 (≠ A273)
binding phosphoaminophosphonic acid-adenylate ester: N163 (= N150), R165 (= R152), K225 (≠ E203), G227 (= G212), S228 (= S213), G230 (= G215), A231 (= A216), A244 (≠ G229), Y245 (= Y230), V249 (= V234), T253 (≠ V238), G254 (= G239), A255 (≠ S240), G256 (= G241), D257 (= D242), M259 (≠ F244), I281 (≠ C266), S284 (≠ G269), I288 (≠ A273)
binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: A32 (≠ G23), G33 (= G24), S34 (≠ A25), N37 (= N28), Y89 (≠ V80), L103 (≠ I94), Y105 (vs. gap), R107 (vs. gap), I136 (≠ S123), R165 (= R152), T253 (≠ V238), G254 (= G239), D257 (= D242)
binding 2-keto-3-deoxygluconate: G33 (= G24), S34 (≠ A25), N37 (= N28), Y89 (≠ V80), L103 (≠ I94), Y105 (vs. gap), R107 (vs. gap), I136 (≠ S123), R165 (= R152), T253 (≠ V238), G254 (= G239), D257 (= D242)

Sites not aligning to the query:

7cf8D Pfkb(mycobacterium marinum) (see paper)
23% identity, 83% coverage: 4:249/295 of query aligns to 3:237/282 of 7cf8D

query
sites
7cf8D

K

R

A

G

V

L

I

V

F

I

G

G

E

E

M

A

L

L

W

I

D

D

C

I

F

V

-

D

-

G

P

P

D

D

K

P

-

A

Q

E

L

Y

P

V

G

G

A

S

P

P

M

L

N

N

V

V

A

A

L

V

H

G

L

L

Q

A

H

R

L

L

G

G

I

R

T

D

T

V

T

D

F

L

I

L

S

T

K

H

I

I

G

G

S
x
R

D

D

S

A

L

R

G

G

A

R

D

R

L

I

L

A

S

E

F

Y

V

I

Q

E

K

S

N

S

G

G

L

V

N

Q

G

-

D

-

F

-

V

-

Q

-

R

-

D

-

T

-

A

-

H

-

E

-

T

-

S

-

R

-

V

L

V

V

V
x
S

D
x
G

N

S

S

Q

D

T

K

A

E

D

N

R

I

T

K

P

Y

T

E

A

I

T

V

A

K

R

P

T

V

Y

A

A

W

F

D

D

F

-

M

L

E

E

W

W

N

Q

T

I

A

P

I

D

Q

T

E

P

K

P

V

V

D

A

E

P

A

P

D

L

V

L

F

V

V

H

F

T

G

G

S

S

L

I

A

A

R

A

S

R

S

E

Q

P

S

G

Q

C

N

L

T

A

L

V

F

A

R

A

L

L

E

D

-

A

-

Y

-

R

T

A

S

A

T

A

L

T

K

V

V

S

L

F

D

D

I

P

N

N

L

V

R

R

P

P

-

S

-

L

-

S

-

A

-

D

P

P

H

D

Y

L

S

T

T

R

K

E

V

R

L

I

E

Q

R

R

L

L

L

V

K

E

N

R

T

S

D

D

V

I

L

I

K

K

I

A

N

S

E

A

D

E

E

D

L

L

E

H

I

W

L

I

I

D

E

P

M

T

S

Q

A

P

L

P

D

E

K

Q

N

T

E

A

E

R

K

A

A

W

L

L

S

A

A

C

I

-

V

-

D

-

R

-

Y

-

E

G

L

P

Q

A

L

I

V

V

C

A

M

L

T

T

K

L

G

G

S

D

A

Q

G

G

A

A

I

V

I

A

Y

F

D

C

G

A

R

A

E

G

F

P

Y

A

R

S

H

V

P

P

G

A

Y

Q

Q

P

V

-

D

-

V

V

E

V

D

D

T

T

V

V

G

G

S

A

G

G

D

D

A

A

F

F

L

M

S

A

G

G

F

L

I

L

binding phosphate ion: R50 (≠ S48), S70 (≠ V82), G71 (≠ D83)

4wjmA Crystal structure of fructokinase from brucella abortus 2308 with bound amppnp
25% identity, 82% coverage: 9:251/295 of query aligns to 12:264/312 of 4wjmA

query
sites
4wjmA

G

G

E

E

M

A

L

L

W

I

D

D

C

M

F

L

P

P

D

R

-

E

-

T

-

G

-

E

-

T

-

A

-

F

K

Q

Q

P

L

F

P

A

G

G

A

S

P

V

M

F

N

N

V

T

A

A

L

I

H

A

L

L

Q

G

H

R

L

L

G

G

I

V

T

P

T

T

T

G

F

F

I

F

S

S

K

G

I

I

G

S

S

S

D

D

S

F

L

F

G

G

A

D

D

V

L

L

L

R

S

D

F

T

V

L

Q

A

K

R

N

S

G

N

L

V

N

D

G

Y

D

S

F

F

V

A

-

A

-

I

-

S

Q

N

R

R

D

P

T

T

A

T

H

L

E

A

T

F

S

V

R

R

V

L

V

V

V

-

D

D

N

G

S

Q

D

A

K

R

E

-

N

-

I

-

K

-

Y

-

E

-

I

-

V

-

K

-

P

-

V

-

A

-

W

Y

D

A

F

F

M

Y

E

D

W

E

N

N

T

T

A

A

I

G

Q

R

-

M

-

L

-

S

-

R

-

N

-

D

-

M

E

P

K

Y

V

V

D

D

E

E

A

T

-

I

D

S

V

A

F

M

V

L

F

F

G

G

S

C

L

I

A

S

A

L

R

I

S

S

S

E

Q

P

S

C

Q

G

N

S

-

V

-

Y

-

E

T

T

L

L

F

L

R

A

L

R

L

E

E

A

T

P

S

N

T

R

L

V

K

M

V

F

L

L

D

D

I

P

N
|
N

L

I

R
|
R

P

A

P

N

H

L

Y

I

S

T

T

V

K

R

-

K

-

T

-

H

-

L

-

T

V

R

L

M

E

K

R

R

L

M

L

I

K

A

N
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