SitesBLAST

O95571 Persulfide dioxygenase ETHE1, mitochondrial; Ethylmalonic encephalopathy protein 1; Hepatoma subtracted clone one protein; Sulfur dioxygenase ETHE1; EC 1.13.11.18 from Homo sapiens (Human) (see 4 papers)
31% identity, 89% coverage: 17:209/217 of query aligns to 38:213/254 of O95571

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O95571

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L55 (≠ K36) to P: in EE; reduces protein stability; dbSNP:rs182983506
H79 (= H60) binding
H135 (= H135) binding
T152 (≠ G152) to I: in EE; reduces protein stability, iron content and enzyme activity; dbSNP:rs1317633085
D154 (= D154) binding
T164 (= T164) to K: in EE; reduces protein stability; dbSNP:rs1268640442
D196 (≠ G196) to N: in EE; reduces protein stability and affinity for substrate; dbSNP:rs763799125

Sites not aligning to the query:

1:7 modified: transit peptide, Mitochondrion

4chlB Human ethylmalonic encephalopathy protein 1 (hethe1) (see paper)
31% identity, 89% coverage: 17:209/217 of query aligns to 22:197/237 of 4chlB

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active site: H63 (= H60), H65 (= H62), D67 (= D64), H68 (= H65), H119 (= H135), D138 (= D154), H179 (= H195)
binding fe (iii) ion: H63 (= H60), H119 (= H135), D138 (= D154)

5ve5A Crystal structure of persulfide dioxygenase rhodanese fusion protein with rhodanese domain inactivating mutation (c314s) from burkholderia phytofirmans in complex with glutathione (see paper)
31% identity, 90% coverage: 17:212/217 of query aligns to 19:197/350 of 5ve5A

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active site: R27 (≠ K24), H60 (= H60), H62 (= H62), D64 (= D64), H65 (= H65), H116 (= H135), D135 (= D154), H176 (= H195)
binding fe (iii) ion: H60 (= H60), H116 (= H135), D135 (= D154)
binding glutathione: H116 (= H135), D135 (= D154), G143 (= G162), R144 (= R163), Y178 (vs. gap)

Sites not aligning to the query:

binding glutathione: 214, 217, 218

Q9C8L4 Persulfide dioxygenase ETHE1 homolog, mitochondrial; Glyoxalase II; Glx II; Sulfur dioxygenase ETHE1; EC 1.13.11.18 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
30% identity, 93% coverage: 16:216/217 of query aligns to 66:257/294 of Q9C8L4

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H110 (= H60) binding
H166 (= H135) binding
D191 (= D154) binding

2gcuA X-ray structure of gene product from arabidopsis thaliana at1g53580 (see paper)
30% identity, 92% coverage: 17:216/217 of query aligns to 18:208/244 of 2gcuA

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active site: H61 (= H60), H63 (= H62), D65 (= D64), H66 (= H65), H117 (= H135), D142 (= D154), H183 (= H195)
binding fe (ii) ion: H61 (= H60), H117 (= H135), D142 (= D154)

6sg9FL uS3m (see paper)
25% identity, 92% coverage: 10:208/217 of query aligns to 60:294/320 of 6sg9FL

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V

Y

I

I

W

F

V

S

P

P

G

G

H
|
H

A

S

P

P

G

G

H

H

V

M

F

L

Y

H

H

I

P

P

E

T

S

E

K

R

T

I

C

L

I

F

G

S

G

G

D
|
D

T

L

L

L

F

F

Q

F

G

N

S
x
K

I

V

G

G

R

R

T

V

D

D

L

L

P

P

G

W

G

A

D

T

H

G

Q

V

T

R

L

L

L

A

D

E

A

S

I

L

K

R

A

L

K

-

L

L

F

E

T

A

L

L

P

P

D

D

D

N

V

T

K

V

V

V

H

V

P

P

G

G

H
|
H

G
|
G

P
x
R

A

M

T

T

L

T

I

L

G

G

H

R

E

E

K

R

I

R

H

E

N

N

binding zinc ion: H108 (= H60), H110 (= H62), D112 (= D64), H222 (= H135), D241 (= D154), D241 (= D154), H281 (= H195)
binding : D63 (≠ Q13), Y67 (= Y17), K247 (≠ S160), G282 (= G196), R283 (≠ P197)

Sites not aligning to the query:

binding zinc ion: 320, 320
binding : 40, 43, 45, 57, 58, 319

Q9SID3 Hydroxyacylglutathione hydrolase 2, mitochondrial; Glyoxalase II; Glx II; EC 3.1.2.6 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
30% identity, 82% coverage: 17:195/217 of query aligns to 85:239/324 of Q9SID3

query
sites
Q9SID3

Y

Y

V

I

L

L

Y

H

D

D

-

E

D

D

T

T

K

G

E

T

A

V

V

G

I

V

D

D

P

P

G

S

C

-

Y

-

A

-

K

-

E

-

E

E

Q

A

E

E

T

P

L

I

K

I

Q

D

F

S

L

L

Q

K

S

R

N

S

D

G

L

R

R

N

P

L

V

T

R

Y

L

I

L

L

N

N

T

T

H
|
H

C

H

H
|
H

I

Y

D
|
D

H
|
H

V

T

L

G

G

G

N

N

F

L

F

E

I

L

N

K

Q

D

N

R

Y

Y

G

G

L

A

P

K

L

V

E

-

I

-

H

-

P

-

K

-

D

-

E

-

P

-

V

-

L

-

M

-

A

-

V

I

G

G

S

S

Y

A

A

M

S

D

N

K

Y

D

G

R

F

I

P

P

A

G

Y

I

T

-

P

-

C

-

K

-

A

-

E

D

K

M

Y

A

L

L

E

K

E

D

G

G

D

D

K

K

V

W

T

M

F

F

G

A

E

G

T

H

D

E

L

V

E

H

V

V

I

M

W

D

V

T

P

P

G

G

H
|
H

A

T

P

K

G

G

H

H

V

I

V

S

F

L

Y

Y

H

F

P

P

E

G

S

S

K

R

T

A

C

I

I

F

G

T

G

G

D
|
D

T

T

L

M

F

F

Q

S

G

L

S

S

I

C

G

G

R

K

T

-

D

-

L

L

P

F

G

E

G

G

D

T

H

P

Q

K

T

Q

L

M

L

L

D

A

A

S

I

L

K

Q

A

-

K

K

L

I

F

T

T

S

L

L

P

P

D

D

V

T

K

S

V

I

H

Y

P

C

G

G

H
|
H

H124 (= H60) binding
H126 (= H62) binding
D128 (= D64) binding
H129 (= H65) binding
H182 (= H135) binding
D201 (= D154) binding ; binding
H239 (= H195) binding

2q42A Ensemble refinement of the protein crystal structure of glyoxalase ii from arabidopsis thaliana gene at2g31350 (see paper)
30% identity, 83% coverage: 16:195/217 of query aligns to 14:169/254 of 2q42A

query
sites
2q42A

C

A

Y

Y

V

I

L

L

Y

H

D

D

-

E

D

D

T

T

K

G

E

T

A

V

V

G

I

V

D

D

P

P

G

S

C

-

Y

-

A

-

K

-

E

-

E

E

Q

A

E

E

T

P

L

I

K

I

Q

D

F

S

L

L

Q

K

S

R

N

S

D

G

L

R

R

N

P

L

V

T

R

Y

L

I

L

L

N

N

T

T

H
|
H

C

H

H
|
H

I

Y

D
|
D

H
|
H

V

T

L

G

G

G

N

N

F

L

F

E

I

L

N

K

Q

D

N

R

Y

Y

G

G

L

A

P

K

L

V

E

-

I

-

H

-

P

-

K

-

D

-

E

-

P

-

V

-

L

-

M

-

A

-

V

I

G

G

S

S

Y

A

A

M

S

D

N

K

Y

D

G

R

F

I

P

P

A

G

Y

I

T

-

P

-

C

-

K

-

A

-

E

D

K

M

Y

A

L

L

E

K

E

D

G

G

D

D

K

K

V

W

T

M

F

F

G

A

E

G

T

H

D

E

L

V

E

H

V

V

I

M

W

D

V

T

P

P

G

G

H
|
H

A

T

P

K

G

G

H

H

V

I

V

S

F

L

Y

Y

H

F

P

P

E

G

S

S

K

R

T

A

C

I

I

F

G

T

G

G

D
|
D

T

T

L

M

F

F

Q

S

G

L

S

S

I

C

G

G

R

K

T

-

D

-

L

L

P

F

G

E

G

G

D

T

H

P

Q

K

T

Q

L

M

L

L

D

A

A

S

I

L

K

Q

A

-

K

K

L

I

F

T

T

S

L

L

P

P

D

D

V

T

K

S

V

I

H

Y

P

C

G

G

H
|
H

active site: H54 (= H60), H56 (= H62), D58 (= D64), H59 (= H65), H112 (= H135), D131 (= D154), H169 (= H195)
binding fe (iii) ion: H54 (= H60), D58 (= D64), H59 (= H65), D131 (= D154), H169 (= H195)
binding zinc ion: H54 (= H60), H56 (= H62), H112 (= H135), D131 (= D154)

4ysbA Crystal structure of ethe1 from myxococcus xanthus (see paper)
28% identity, 93% coverage: 16:216/217 of query aligns to 15:195/225 of 4ysbA

query
sites
4ysbA

C

T

Y

Y

V

L

L

I

Y

G

D

D

D

E

-

A

T

T

K

R

E

Q

A

A

V

V

I

L

I

I

D

D

P

P

G

-

C

-

Y

-

A

V

K

L

E

E

E

Q

Q

V

E

D

T

R

L

D

K

L

Q

Q

F

M

L

V

Q

A

S

E

N

L

D

D

L

L

R

T

P

L

V

T

R

H

L

V

L

F

N

D

T

T

H
|
H

C

V

H
|
H

I

A

D
|
D

H
|
H

V

I

L

T

G

A

N

S

F

G

F

A

I

L

N

R

Q

E

N

R

Y

-

G

-

L

-

P

-

L

-

E

-

I

-

H

-

P

-

K

-

D

-

E

-

P

-

V

-

L

-

M

-

A

-

V

-

G

-

S

T

Y

Q

A

A

S

T

N

V

Y

V

G

G

F

S

P

V

A

N

Y

G

T

A

P

S

C

C

K

-

A

A

E

N

K

V

Y

Q

L

V

E

R

E

H

G

G

D

D

K

E

V

V

T

R

F

V

G

G

E

Q

T

L

D

V

L

F

E

Q

V

V

I

L

W

A

V

T

P

P

G

G

H
|
H

A

T

P

D

G

D

H

S

V

I

V

S

F

Y

Y

L

H

L

P

G

E

D

S

R

K

-

T

-

C

V

I

F

G

T

G

G

D
|
D

T

A

L

L

F

L

Q

V

G

R

S

G

I

N

G

G

R

R

T

T

D

D

L

F

P

Q

G

N

G

G

D

N

H

A

Q

S

T

Q

L

L

L

Y

D

D

A

S

I

L

K

T

A

R

K

V

L

L

F

F

T

T

L

L

P

P

D

D

D

E

V

T

K

L

V

V

H

Y

P

P

G

G

H
|
H

G

D

-

Y

-

K

-

G

-

R

P

T

A

V

T

T

L

S

I

I

G

A

H

E

E

E

K

K

I

R

H

H

N

N

P

P

F

R

V

V

G

A

K

G

N

K

A

S

Q

R

active site: H57 (= H60), H59 (= H62), D61 (= D64), H62 (= H65), H112 (= H135), D129 (= D154), H170 (= H195)
binding fe (iii) ion: H57 (= H60), H112 (= H135), D129 (= D154)

3tp9A Crystal structure of alicyclobacillus acidocaldarius protein with beta-lactamase and rhodanese domains
29% identity, 86% coverage: 22:208/217 of query aligns to 24:223/473 of 3tp9A

query
sites
3tp9A

D

E

T

T

K

G

E

E

A

A

V

C

I

V

I

I

D

D

P

P

G

A

C

R

Y

D

A

V

K

E

E

P

E

Y

Q

L

E

L

T

T

L

A

K

K

Q

R

F

-

L

-

Q

-

S

-

N

E

D

G

L

L

R

R

P

I

V

V

R

A

L

A

L

L

N

E

T

T

H
|
H

C

I

H
|
H

I

A

D
|
D

H
x
F

V

V

L

S

G

G

N

A

F

R

F

E

I

M

N

A

Q

D

N

R

Y

A

G

G

L

A

P

A

L

I

E

C

I

V

H

S

P

D

K

E

D

G

E

P

P

P

V

-

L

-

M

-

A

-

V

-

G

-

S

E

Y

W

A

K

S

S

N

E

Y

Y

G

-

F

V

P

K

A

A

Y

Y

T

P

P

-

C

-

K

-

A

-

E

H

K

R

Y

L

L

L

E

K

E

D

G

G

D

D

K

E

V

L

T

H

F

F

G

G

E

N

T

V

D

R

L

I

E

V

V

V

I

M

W

H

V

T

P

P

G

G

H
|
H

A

T

P

P

G

E

H

H

V

V

V

S

F

Y

Y

L

H

L

P

Y

E

D

S

G

K

K

T

T

C

S

-

P

-

D

-

V

-

P

-

M

-

A

-

L

I

F

G

S

G

G

D
|
D

T

F

L

V

F

F

Q

V

G

G

S

D

I

V

G

G

R

R

T

P

D

D

L

L

-

L

-

E

-

R

-

V

-

A

-

G

P

E

G

S

G

G

D

S

H

S

Q

E

T

A

L

L

L

A

D

R

A

Q

I

M

-

F

-

R

-

S

K

L

A

R

K

K

L

F

F

E

T

A

L

L

P

P

D

D

D

H

V

V

K

Q

V

V

H

L

P

P

G

A

H

H

G

G

-

A

-

G

-

S

-

A

-

C

-

G

-

K

-

A

-

L

-

G

-

A

-

V

P

P

A

S

T

S

L

T

I

V

G

G

H

Y

E

E

K

K

I

L

H

V

N

N

active site: H58 (= H60), H60 (= H62), D62 (= D64), F63 (≠ H65), H122 (= H135), D148 (= D154)
binding zinc ion: H58 (= H60), H122 (= H135), D148 (= D154)

5wcmA Crystal structure of the complex between class b3 beta-lactamase bjp-1 and 4-nitrobenzene-sulfonamide - new refinement (see paper)
38% identity, 38% coverage: 56:138/217 of query aligns to 66:149/263 of 5wcmA

query
sites
5wcmA

L

I

L

L

N

N

T

T

H
|
H

C

A

H
|
H

I
x
L

D
|
D

H
|
H

V

T

L

G

G

G

N

F

F

A

F
x
E

I

I

N

K

Q

K

N
x
E

Y

T

G

G

L

A

P

Q

L

L

E

V

I

A

H

G

P

E

K

R

D

D

E

K

P

P

V

L

L

L

M

E

A

G

V

-

G

G

S

Y

Y

Y

A

P

-

G

-

D

-

E

-

K

-

N

S

E

N

D

Y
x
L

G

A

F

F

P

P

A

A

Y

-

T

-

P

-

C

V

K

K

A

V

E

D

K

R

Y

A

L

V

E

K

E
|
E

G

G

D

D

K

R

V

V

T

T

F

L

G

G

E

D

T

T

D

T

L

L

E

T

V

A

I
x
H

W

A

V

T

P

P

G

G

H
|
H

A

S

P

P

G

G

active site: H70 (= H60), H72 (= H62), D74 (= D64), H75 (= H65), H146 (= H135)
binding 4-nitrobenzenesulfonamide: L73 (≠ I63), D74 (= D64), L114 (≠ Y100), H146 (= H135)
binding zinc ion: H70 (= H60), H72 (= H62), D74 (= D64), H75 (= H65), E81 (≠ F71), E85 (≠ N75), E127 (= E116), H141 (≠ I130), H146 (= H135)

Sites not aligning to the query:

active site: 177, 211
binding 4-nitrobenzenesulfonamide: 3, 176, 211
binding zinc ion: 8, 50, 55, 198, 211

5nggA Crystal structure of the subclass b3 metallo-beta-lactamase bjp-1 in complex with acetate anion
37% identity, 38% coverage: 56:138/217 of query aligns to 77:160/274 of 5nggA

query
sites
5nggA

L

I

L

L

N

N

T

T

H
|
H

C

A

H
|
H

I

L

D
|
D

H
|
H

V

T

L

G

G

G

N

F

F

A

F

E

I

I

N

K

Q

K

N

E

Y

T

G

G

L

A

P

Q

L

L

E

V

I

A

H

G

P

E

K

R

D

D

E

K

P

P

V

L

L

L

M

E

A

-

V

-

G

G

S

G

Y

Y

-

Y

-

P

-

G

-

D

-

E

-

K

A

N

S

E

N

D

Y

L

G

A

F

F

P

P

A

A

Y

-

T

-

P

-

C

V

K

K

A

V

E

D

K

R

Y

A

L

V

E

K

E
|
E

G

G

D

D

K

R

V

V

T

T

F

L

G

G

E

D

T

T

D

T

L

L

E

T

V

A

I
x
H

W

A

V

T

P

P

G

G

H
|
H

A

S

P

P

G

G

active site: H81 (= H60), H83 (= H62), D85 (= D64), H86 (= H65), H157 (= H135)
binding zinc ion: H81 (= H60), H83 (= H62), D85 (= D64), H86 (= H65), E138 (= E116), H152 (≠ I130), H157 (= H135)

Sites not aligning to the query:

active site: 188, 222
binding zinc ion: 209, 222

4efzA Crystal structure of a hypothetical metallo-beta-lactamase from burkholderia pseudomallei
24% identity, 82% coverage: 17:195/217 of query aligns to 20:216/295 of 4efzA

query
sites
4efzA

Y

Y

V

L

L

L

Y

F

D

D

D

S

-

G

T

S

K

G

E

E

A

C

V

A

I

L

I

I

D

D

P

S

-

V

-

L

-

D

-

Y

-

D

-

P

-

K

-

S

G

G

C

R

Y

T

A

R

K

T

E

A

E

S

Q

A

E

D

T

Q

L

L

K

I

Q

A

F

R

L

V

Q

A

S

A

N

L

D

G

L

A

R

R

P

V

V

R

R

W

L

L

N

E

T

T

H
|
H

C

V

H
|
H

I

A

D
|
D

H
|
H

V

L

L

S

G

A

N

A

F

P

F

Y

I

L

N

K

Q

T

N

R

Y

V

G

G

L

G

P

E

L

I

E

A

I

I

H

G

P

R

K

H

D

V

E

T

P

R

V

V

L

Q

M

D

A

V

V

F

G

G

S

K

Y

L

A

F

S

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N

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Y

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G

P

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Q

A

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L

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G

D

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H

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A

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D

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T

L

L

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D

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G

G

-

T

-

A

R

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D

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D

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H

H

active site: H72 (= H60), H74 (= H62), D76 (= D64), H77 (= H65)
binding calcium ion: H72 (= H60), H147 (= H135), D174 (= D154)

2p18A Crystal structure of the leishmania infantum glyoxalase ii (see paper)
35% identity, 49% coverage: 56:162/217 of query aligns to 64:162/283 of 2p18A

query
sites
2p18A

L

I

L

L

N

S

T

T

H
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H

C

K

H
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H

I

W

D
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D

H
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H

V

S

L

G

G

G

N

N

F

-

I

-

N

-

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-

N

-

Y

A

G

K

L

L

P

K

L

A

E

E

I

L

H

E

P

A

K

M

D

N

E

V

P

P

V

V

L

V

M

V

A

V

V

G

G

G

S

A

Y

-

A

-

S

-

N

N

Y

D

G

S

F

I

P

P

A

A

Y

V

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T

P

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C

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A

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E

E

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G

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D

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L

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H

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I

G

G

active site: H68 (= H60), H70 (= H62), D72 (= D64), H73 (= H65), H129 (= H135), D154 (= D154)
binding spermidine: I161 (= I161)
binding zinc ion: H68 (= H60), H70 (= H62), D72 (= D64), H73 (= H65), H129 (= H135), D154 (= D154), D154 (= D154)

Sites not aligning to the query:

8ewoA Crystal structure of putative glyoxylase ii from pseudomonas aeruginosa
36% identity, 38% coverage: 114:195/217 of query aligns to 92:171/259 of 8ewoA

query
sites
8ewoA

L

L

E

E

E

D

G

G

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K

R

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H

H

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Y

Y

Y

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D

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T

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L

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F

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G

G

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R

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D

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P

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G

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M

H

H

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H

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L

L

L

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A

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I

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K

-

A

A

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R

L

L

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A

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L

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P

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A

D

N

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V

H

Y

P

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H
|
H

binding zinc ion: H113 (= H135), D133 (= D154), D133 (= D154), H171 (= H195)

Sites not aligning to the query:

binding zinc ion: 57, 59, 61, 62

Query Sequence

>Echvi_2827 FitnessBrowser__Cola:Echvi_2827
MLNVQTFTFNPFQENCYVLYDDTKEAVIIDPGCYAKEEQETLKQFLQSNDLRPVRLLNTH
CHIDHVLGNFFINQNYGLPLEIHPKDEPVLMAVGSYASNYGFPAYTPCKAEKYLEEGDKV
TFGETDLEVIWVPGHAPGHVVFYHPESKTCIGGDTLFQGSIGRTDLPGGDHQTLLDAIKA
KLFTLPDDVKVHPGHGPATLIGHEKIHNPFVGKNAQF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory