SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_2865 FitnessBrowser__Cola:Echvi_2865 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7xreC Crystal structure of dgpa
27% identity, 37% coverage: 88:271/492 of query aligns to 33:213/363 of 7xreC

query
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7xreC

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binding nicotinamide-adenine-dinucleotide: D38 (= D93), I39 (≠ V94), T78 (= T135), P79 (= P136), N80 (≠ D137), H83 (= H140), E100 (= E157), K101 (= K158), P102 (= P159), F170 (≠ N227), Q176 (vs. gap), H187 (≠ D239)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 13, 14, 313, 314

7xr9A Crystal structure of dgpa with glucose (see paper)
29% identity, 32% coverage: 116:271/492 of query aligns to 51:203/344 of 7xr9A

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binding beta-D-glucopyranose: K91 (= K158), R150 (≠ G217), W157 (= W224), F160 (≠ N227), D173 (≠ E235), H177 (≠ D239)
binding nicotinamide-adenine-dinucleotide: D51 (= D116), Y52 (≠ F117), C67 (≠ A134), T68 (= T135), P69 (= P136), H73 (= H140), E90 (= E157), K91 (= K158), P92 (= P159), Q119 (= Q186), V159 (= V226), F160 (≠ N227), Q166 (vs. gap)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 13, 14, 15

3q2kC Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
26% identity, 38% coverage: 57:243/492 of query aligns to 3:195/347 of 3q2kC

query
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active site: K100 (= K158), H188 (≠ T236)
binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R17 (≠ N71), K100 (= K158), W159 (≠ G217), T160 (≠ R218), R161 (= R219), Y165 (vs. gap), N184 (≠ P232), Q185 (≠ A233), H188 (≠ T236)
binding 1,4-dihydronicotinamide adenine dinucleotide: G16 (= G70), R17 (≠ N71), I18 (≠ R72), D40 (= D93), T41 (≠ V94), A76 (= A134), T77 (= T135), S79 (≠ D137), H82 (= H140), Q85 (≠ V143), E99 (= E157), K100 (= K158), Q128 (= Q186), W171 (= W221), R172 (≠ H222)

Sites not aligning to the query:

binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: 243, 244

3q2kK Crystal structure of the wlba dehydrogenase from bordetella pertussis in complex with nadh and udp-glcnaca (see paper)
26% identity, 37% coverage: 61:243/492 of query aligns to 2:190/322 of 3q2kK

query
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active site: K95 (= K158), H183 (≠ T236)
binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: R12 (≠ N71), K95 (= K158), T155 (≠ R218), R156 (= R219), Y160 (vs. gap), N179 (≠ P232), Q180 (≠ A233), H183 (≠ T236)
binding 1,4-dihydronicotinamide adenine dinucleotide: G9 (= G68), G11 (= G70), R12 (≠ N71), I13 (≠ R72), D35 (= D93), T36 (≠ V94), T72 (= T135), P73 (= P136), S74 (≠ D137), L76 (≠ S139), H77 (= H140), E94 (= E157), K95 (= K158), Q123 (= Q186), W166 (= W221), R167 (≠ H222), H183 (≠ T236)

Sites not aligning to the query:

binding (2S,3S,4R,5R,6R)-5-acetamido-6-[[[(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-3,4-dihydroxy-oxolan-2-yl]methoxy-hydroxy-phosphoryl]oxy-hydroxy-phosphoryl]oxy-3,4-dihydroxy-oxane-2-carboxylic acid: 239

6t2bB Glycoside hydrolase family 109 from akkermansia muciniphila in complex with galnac and NAD+.
27% identity, 30% coverage: 61:208/492 of query aligns to 33:185/439 of 6t2bB

query
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binding nicotinamide-adenine-dinucleotide: G40 (= G68), G43 (≠ N71), R44 (= R72), D65 (= D93), L66 (≠ V94), D93 (= D116), S111 (≠ A134), T112 (= T135), W114 (≠ D137), H117 (= H140), E134 (= E157), V135 (≠ K158), N163 (≠ M183)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 205, 206, 209, 222, 225, 370

3m2tA The crystal structure of dehydrogenase from chromobacterium violaceum
29% identity, 25% coverage: 62:184/492 of query aligns to 4:123/342 of 3m2tA

query
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E

E

L

T

L

L

M

I

K

D

A

A

A

A

E

R

K

R

Y

S

G

D

V

V

V

V

T

S

Q

G

M

V

G

G

binding nicotinamide-adenine-dinucleotide: G10 (= G68), G12 (= G70), A13 (≠ N71), Q14 (≠ R72), D36 (= D93), S37 (≠ V94), R41 (≠ K107), A73 (= A134), G74 (≠ T135), L78 (≠ S139), E96 (= E157), K97 (= K158)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 125, 290

3ceaA Crystal structure of myo-inositol 2-dehydrogenase (np_786804.1) from lactobacillus plantarum at 2.40 a resolution
25% identity, 29% coverage: 65:208/492 of query aligns to 8:149/342 of 3ceaA

query
sites
3ceaA

A

A

C

I

V

I

G
|
G

I

L

G
|
G

N
x
R

R
x
L

G

G

E

E

Q

R

-

H

V

A

I

R

N

H

A

L

I

V

D

N

E

K

T

I

G

Q

Q

G

A

V

N

K

I

L

V

V

T

A

L

A

C

C

D

A

V
x
L

D

D

M

-

G

-

A

S

E

N

H
x
Q

T

L

L

E

P

W

S

A

M

K

N

N

K

E

F

L

P

G

K

V

A

E

T

T

R

T

F

Y

Q

T

D

N

F

Y

R

K

E

D

M

M

L

I

D

D

K

-

D

-

S

T

Q

E

H

N

F

I

D

D

A

A

V

I

M

F

V

I

A
x
V

T
x
A

P
|
P

D
x
T

F

P

S
x
F

H
|
H

F

P

P

E

V
x
M

T

T

M

I

A

Y

A

A

M

M

A

N

A

A

G

G

K

L

G

N

V

V

Y

F

V

C

E
|
E

K
|
K

P

P

L

L

A

G

R

L

T

D

F

F

H

N

E

E

I

V

E

D

L

E

L

M

M

A

K

K

A

V

A

I

E

K

K

S

Y

H

-

P

G

N

V

Q

V

I

T

F

Q

Q

M

S

G

G

N

F

Q
x
M

G

R

H

R

S

Y

E

D

A

D

N

S

Y

Y

F

R

Q

Y

F

A

K

K

A

K

W

I

V

V

D

D

A

N

G

G

I

D

I

I

K

G

D

K

V

I

active site: K98 (= K158)
binding nicotinamide-adenine-dinucleotide: G11 (= G68), G13 (= G70), R14 (≠ N71), L15 (≠ R72), L38 (≠ V94), Q42 (≠ H100), V74 (≠ A134), A75 (≠ T135), P76 (= P136), T77 (≠ D137), F79 (≠ S139), H80 (= H140), M83 (≠ V143), E97 (= E157), K98 (= K158), M127 (≠ Q186)

Sites not aligning to the query:

active site: 185
binding nicotinamide-adenine-dinucleotide: 169, 185, 286

5a06A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with sorbitol (see paper)
29% identity, 24% coverage: 97:214/492 of query aligns to 46:158/336 of 5a06A

query
sites
5a06A

G

G

A

E

E

Q

H

Y

T

G

L

I

P

P

S

E

M

T

N

H

K

R

F

Y

P

-

K

-

A

-

T

-

R

S

F
x
Y

Q

E

D

T

F

F

R

D

E

R

M

I

L

I

D

D

K

-

D

-

S

N

Q

P

H

D

F

V

D
|
D

A

I

V

V

M

Y

V

V

A
x
I

T
|
T

P
|
P

D
x
N

F

S

S
x
L

H
|
H

F

R

P

P

V

F

T

T

M

E

A

R

A

A

M

A

A

R

A

A

G

G

K

K

G
x
H

V

V

Y

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

R

N

T

T

F

V

H

A

E

D

I

C

E

E

L

A

L

M

M

I

K

A

A

A

A

C

E

K

K

K

Y

A

G

G

V
x
R

V

K

T
x
L

Q

M

M

I

G

G

N

Y

Q
x
R

G

S

H

R

S

F

E

Q

A

A

N

H

Y

N

F

I

Q

E

F

A

K

I

A

K

W

L

V

V

D

R

A

D

G

G

I

A

I

L

K

G

D

P

V

V

-

R

T

T

A

V

I

V

T

T

C

D

H

H

active site: K101 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y59 (≠ F114), I77 (≠ A134), T78 (= T135), P79 (= P136), N80 (≠ D137), L82 (≠ S139), H83 (= H140), E100 (= E157), K101 (= K158), R129 (≠ Q186)
binding sorbitol: D72 (= D129), H96 (≠ G153), K101 (= K158), R122 (≠ V179), R122 (≠ V179), L124 (≠ T181)

Sites not aligning to the query:

active site: 186
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 11, 12, 13, 14, 36, 37, 38, 41, 168, 169, 186, 264
binding sorbitol: 2, 160, 169, 182, 186, 287, 296, 299, 306, 310, 311

5a03C Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
29% identity, 24% coverage: 97:214/492 of query aligns to 46:158/336 of 5a03C

query
sites
5a03C

G

G

A

E

E

Q

H

Y

T

G

L

I

P

P

S

E

M

T

N

H

K

R

F

Y

P

-

K

-

A

-

T

-

R

S

F
x
Y

Q

E

D

T

F

F

R

D

E

R

M

I

L

I

D

D

K

-

D

-

S

N

Q

P

H

D

F

V

D

D

A

I

V

V

M

Y

V

V

A
x
I

T
|
T

P
|
P

D
x
N

F

S

S
x
L

H
|
H

F

R

P

P

V

F

T

T

M

E

A

R

A

A

M

A

A

R

A

A

G

G

K

K

G

H

V

V

Y

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

R

N

T

T

F

V

H

A

E

D

I

C

E

E

L

A

L

M

M

I

K

A

A

A

A

C

E

K

K

K

Y

A

G

G

V

R

V

K

T

L

Q

M

M

I

G

G

N

Y

Q
x
R

G

S

H

R

S

F

E

Q

A

A

N

H

Y

N

F

I

Q

E

F

A

K

I

A

K

W

L

V

V

D

R

A

D

G

G

I

A

I

L

K

G

D

P

V

V

-

R

T

T

A

V

I

V

T

T

C

D

H

H

active site: K101 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y59 (≠ F114), I77 (≠ A134), T78 (= T135), P79 (= P136), N80 (≠ D137), L82 (≠ S139), H83 (= H140), E100 (= E157), K101 (= K158), R129 (≠ Q186)
binding beta-D-xylopyranose: K101 (= K158)

Sites not aligning to the query:

active site: 186
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 14, 36, 37, 38, 41, 168, 169, 186, 264
binding beta-D-xylopyranose: 160, 169, 182, 186

5a05A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with maltotriose (see paper)
29% identity, 24% coverage: 97:214/492 of query aligns to 45:157/335 of 5a05A

query
sites
5a05A

G

G

A

E

E

Q

H

Y

T

G

L

I

P

P

S

E

M

T

N

H

K

R

F

Y

P

-

K

-

A

-

T

-

R

S

F
x
Y

Q

E

D

T

F

F

R

D

E

R

M

I

L

I

D

D

K

-

D

-

S

N

Q

P

H

D

F

V

D

D

A

I

V

V

M

Y

V

V

A
x
I

T
|
T

P
|
P

D
x
N

F

S

S
x
L

H
|
H

F

R

P

P

V

F

T

T

M

E

A

R

A

A

M

A

A

R

A

A

G

G

K

K

G

H

V

V

Y

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

R

N

T

T

F

V

H

A

E

D

I

C

E

E

L

A

L

M

M

I

K

A

A

A

A

C

E

K

K

K

Y

A

G

G

V

R

V

K

T

L

Q

M

M

I

G

G

N

Y

Q
x
R

G

S

H

R

S

F

E

Q

A

A

N

H

Y

N

F

I

Q

E

F

A

K

I

A

K

W

L

V

V

D

R

A

D

G

G

I

A

I

L

K

G

D

P

V

V

-

R

T

T

A

V

I

V

T

T

C

D

H

H

active site: K100 (= K158)
binding beta-D-glucopyranose: K100 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y58 (≠ F114), I76 (≠ A134), T77 (= T135), P78 (= P136), N79 (≠ D137), L81 (≠ S139), H82 (= H140), E99 (= E157), K100 (= K158), R128 (≠ Q186)

Sites not aligning to the query:

active site: 185
binding beta-D-glucopyranose: 159, 181, 185
binding alpha-D-glucopyranose: 259, 262
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 35, 36, 37, 40, 167, 168, 185, 263

5a04A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glucose (see paper)
29% identity, 24% coverage: 97:214/492 of query aligns to 45:157/335 of 5a04A

query
sites
5a04A

G

G

A

E

E

Q

H

Y

T

G

L

I

P

P

S

E

M

T

N

H

K

R

F

Y

P

-

K

-

A

-

T

-

R

S

F
x
Y

Q

E

D

T

F

F

R

D

E

R

M

I

L

I

D

D

K

-

D

-

S

N

Q

P

H

D

F

V

D

D

A

I

V

V

M

Y

V

V

A
x
I

T
|
T

P
|
P

D
x
N

F

S

S
x
L

H
|
H

F

R

P

P

V

F

T

T

M

E

A

R

A

A

M

A

A

R

A

A

G

G

K

K

G

H

V

V

Y

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

R

N

T

T

F

V

H

A

E

D

I

C

E

E

L

A

L

M

M

I

K

A

A

A

A

C

E

K

K

K

Y

A

G

G

V

R

V

K

T

L

Q

M

M

I

G

G

N

Y

Q
x
R

G

S

H

R

S

F

E

Q

A

A

N

H

Y

N

F

I

Q

E

F

A

K

I

A

K

W

L

V

V

D

R

A

D

G

G

I

A

I

L

K

G

D

P

V

V

-

R

T

T

A

V

I

V

T

T

C

D

H

H

active site: K100 (= K158)
binding beta-D-glucopyranose: K100 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y58 (≠ F114), I76 (≠ A134), T77 (= T135), P78 (= P136), N79 (≠ D137), L81 (≠ S139), H82 (= H140), E99 (= E157), K100 (= K158), R128 (≠ Q186)

Sites not aligning to the query:

active site: 185
binding beta-D-glucopyranose: 159, 168, 181, 185
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 35, 36, 37, 40, 167, 168, 185, 263

5a03E Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with xylose (see paper)
29% identity, 24% coverage: 97:214/492 of query aligns to 45:157/335 of 5a03E

query
sites
5a03E

G

G

A

E

E

Q

H

Y

T

G

L

I

P

P

S

E

M

T

N

H

K

R

F

Y

P

-

K

-

A

-

T

-

R

S

F
x
Y

Q

E

D

T

F

F

R

D

E

R

M

I

L

I

D

D

K

-

D

-

S

N

Q

P

H

D

F

V

D

D

A

I

V

V

M

Y

V

V

A
x
I

T
|
T

P
|
P

D
x
N

F

S

S

L

H
|
H

F

R

P

P

V

F

T

T

M

E

A

R

A

A

M

A

A

R

A

A

G

G

K

K

G

H

V

V

Y

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

R

N

T

T

F

V

H

A

E

D

I

C

E

E

L

A

L

M

M

I

K

A

A

A

A

C

E

K

K

K

Y

A

G

G

V

R

V

K

T

L

Q

M

M

I

G

G

N

Y

Q
x
R

G

S

H

R

S

F

E

Q

A

A

N
x
H

Y

N

F

I

Q

E

F

A

K

I

A

K

W

L

V

V

D

R

A

D

G

G

I

A

I

L

K

G

D

P

V

V

-

R

T

T

A

V

I

V

T

T

C

D

H

H

active site: K100 (= K158)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y58 (≠ F114), I76 (≠ A134), T77 (= T135), P78 (= P136), N79 (≠ D137), H82 (= H140), E99 (= E157), K100 (= K158), R128 (≠ Q186)
binding beta-D-xylopyranose: K100 (= K158)
binding alpha-D-xylopyranose: H134 (≠ N192)

Sites not aligning to the query:

active site: 185
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 9, 10, 11, 12, 13, 35, 36, 37, 40, 167, 168, 185, 263
binding beta-D-xylopyranose: 159, 168, 181, 185, 205, 207, 209
binding alpha-D-xylopyranose: 268, 279, 280

5a02A Crystal structure of aldose-aldose oxidoreductase from caulobacter crescentus complexed with glycerol (see paper)
29% identity, 24% coverage: 97:214/492 of query aligns to 45:157/335 of 5a02A

query
sites
5a02A

G

G

A

E

E

Q

H

Y

T

G

L

I

P

P

S

E

M

T

N

H

K

R

F

Y

P

-

K

-

A

-

T

-

R

S

F
x
Y

Q

E

D

T

F

F

R

D

E

R

M

I

L

I

D

D

K

-

D

-

S

N

Q

P

H

D

F

V

D

D

A

I

V

V

M

Y

V

V

A
x
I

T
|
T

P
|
P

D

N

F

S

S
x
L

H
|
H

F

R

P

P

V

F

T

T

M

E

A

R

A

A

M

A

A

R

A

A

G

G

K

K

G

H

V

V

Y

M

V

C

E
|
E

K
|
K

P

P

L

M

A

A

R

N

T

T

F

V

H

A

E

D

I

C

E

E

L

A

L

M

M

I

K

A

A

A

A

C

E

K

K

K

Y

A

G

G

V

R

V

K

T

L

Q

M

M

I

G

G

N

Y

Q
x
R

G

S

H

R

S

F

E

Q

A

A

N

H

Y

N

F

I

Q

E

F

A

K

I

A

K

W

L

V

V

D

R

A

D

G

G

I

A

I

L

K

G

D

P

V

V

-

R

T

T

A

V

I

V

T

T

C

D

H

H

active site: K100 (= K158)
binding nadp nicotinamide-adenine-dinucleotide phosphate: Y58 (≠ F114), I76 (≠ A134), T77 (= T135), P78 (= P136), L81 (≠ S139), H82 (= H140), E99 (= E157), K100 (= K158), R128 (≠ Q186)

Sites not aligning to the query:

active site: 185
binding nadp nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 35, 36, 37, 40, 167, 168, 185, 263

7d5nB Crystal structure of inositol dehydrogenase homolog complexed with nadh and myo-inositol from azotobacter vinelandii
41% identity, 12% coverage: 114:174/492 of query aligns to 67:130/389 of 7d5nB

query
sites
7d5nB

F

Y

Q

P

D

D

F

Y

R

R

E

T

M

L

L

F

D

E

K

Q

D

E

S

A

Q

R

H

R

F

P

D

D

-

G

-

I

-

Q

A

A

V

V

M

S

V

V

A

A

T
|
T

P
|
P

D
x
N

F

G

S

T

H
|
H

F

F

P

A

V

I

T

T

M

R

A

A

A

A

M

L

A

E

A

A

G

G

K

L

G

H

V

V

Y

V

V

C

E
|
E

K
|
K

P

P

L

L

A

C

R

F

T

T

F

L

H

E

E

E

I

A

E

E

L

T

L

L

M

R

K

E

A

I

A

A

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: K114 (= K158)
binding 1,4-dihydronicotinamide adenine dinucleotide: T91 (= T135), P92 (= P136), N93 (≠ D137), H96 (= H140), E113 (= E157), K114 (= K158)

Sites not aligning to the query:

binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: 143, 190, 204, 208
binding 1,4-dihydronicotinamide adenine dinucleotide: 17, 19, 22, 23, 24, 46, 47, 189, 190, 208, 332

7d5nA Crystal structure of inositol dehydrogenase homolog complexed with nadh and myo-inositol from azotobacter vinelandii
41% identity, 12% coverage: 114:174/492 of query aligns to 67:130/379 of 7d5nA

query
sites
7d5nA

F

Y

Q

P

D

D

F

Y

R

R

E

T

M

L

L

F

D

E

K

Q

D

E

S

A

Q

R

H

R

F

P

D

D

-

G

-

I

-

Q

A

A

V

V

M

S

V

V

A

A

T
|
T

P
|
P

D
x
N

F

G

S

T

H
|
H

F

F

P

A

V

I

T

T

M

R

A

A

A

A

M

L

A

E

A

A

G

G

K

L

G

H

V

V

Y

V

V

C

E
|
E

K
|
K

P

P

L

L

A

C

R

F

T

T

F

L

H

E

E

E

I

A

E

E

L

T

L

L

M

R

K

E

A

I

A

A

binding 1,4-dihydronicotinamide adenine dinucleotide: T91 (= T135), P92 (= P136), N93 (≠ D137), H96 (= H140), E113 (= E157), K114 (= K158)

Sites not aligning to the query:

binding 1,4-dihydronicotinamide adenine dinucleotide: 17, 19, 22, 23, 24, 46, 47, 185, 186, 199, 323

7d5mA Crystal structure of inositol dehydrogenase homolog complexed with NAD+ from azotobacter vinelandii
41% identity, 12% coverage: 114:174/492 of query aligns to 68:131/389 of 7d5mA

query
sites
7d5mA

F

Y

Q

P

D

D

F

Y

R

R

E

T

M

L

L

F

D

E

K

Q

D

E

S

A

Q

R

H

R

F

P

D

D

-

G

-

I

-

Q

A

A

V

V

M

S

V

V

A

A

T
|
T

P
|
P

D
x
N

F

G

S

T

H
|
H

F

F

P

A

V

I

T

T

M

R

A

A

A

A

M

L

A

E

A

A

G

G

K

L

G

H

V

V

Y

V

V

C

E
|
E

K
|
K

P

P

L

L

A

C

R

F

T

T

F

L

H

E

E

E

I

A

E

E

L

T

L

L

M

R

K

E

A

I

A

A

binding nicotinamide-adenine-dinucleotide: T92 (= T135), P93 (= P136), N94 (≠ D137), H97 (= H140), E114 (= E157), K115 (= K158)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 18, 20, 23, 24, 25, 47, 48, 190, 191, 209, 333

3dtyA Crystal structure of an oxidoreductase from pseudomonas syringae
31% identity, 21% coverage: 114:216/492 of query aligns to 63:166/374 of 3dtyA

query
sites
3dtyA

F

Y

Q

A

D

D

F

Y

R

L

E

S

M

M

L

F

D

E

K

Q

D

E

S

A

Q

R

H

R

F

A

D

D

-

G

-

I

-

Q

A

A

V

V

M

S

V

I

A

A

T

T

P

P

D

N

F

G

S

T

H

H

F

Y

P

S

V

I

T

T

M

K

A

A

A

A

M

L

A

E

A

A

G

G

K

L

G

H

V

V

Y

V

V

C

E

E

K
|
K

P

P

L

L

A

C

R

F

T

T

F

V

H
x
E

E

Q

I

A

E

E

L
x
N

L

L

M

R

K

E

A

L

A

S

E

H

K

K

Y

H

G

N

V

R

V

I

T

V

Q

G

M

V

-

T

-

Y

G

G

N

Y

Q

A

G

G

H

H

S

Q

E

L

A

I

N

E

Y

-

F

-

Q

Q

F

A

K

R

A

E

W

M

V

I

D

A

A

A

G

G

I

E

I

L

K

G

D

D

V

V

T

R

A

M

I

V

T

-

C

-

H

H

M

M

N

Q

active site: K110 (= K158)
binding magnesium ion: E117 (≠ H165), N121 (≠ L169)

Sites not aligning to the query:

active site: 186

6z3cAAA Gfo/Idh/MocA family oxidoreductase (see paper)
25% identity, 33% coverage: 62:221/492 of query aligns to 12:161/379 of 6z3cAAA

query
sites
6z3cAAA

V

V

N

G

L

Y

A

A

C

I

V

V

G

G

I

T

G
|
G

N
x
Y

R
x
F

G

G

E

A

Q

E

V

L

I

G

N

R

A

I

I

M

D

K

E

E

T

Q

G

E

Q

G

A

A

N

R

I

I

V

V

T

A

L

V

C
x
L

D
|
D

V
x
P

D

E

M

N

G

G

A

-

E

-

H

-

T

-

L

-

P

-

S

-

M

-

N

-

K

-

F

-

P

-

K

-

A

-

T

-

R

-

F

-

Q

Q

D

T

F

I

R

A

E

E

M

E

L

L

D

D

K

C

D

D

-

V

-

E

-

T

-

D

-

L

-

D

-

T

-

L

-

Y

-

S

S

R

Q

E

H

D

F

V

D

E

A

A

V

V

M

I

V

V

A
|
A

T
|
T

P
|
P

D
x
N

F

Y

S

L

H
|
H

F

K

P

E

V

P

T

V

M

I

A

K

A

A

M

A

A

E

A

H

G

G

K

V

G

N

V

V

Y

F

V

C

E
|
E

K
|
K

P

P

L

I

A

A

R

L

T

S

F

Y

H

Q

E

D

I

C

E

D

L

E

L

M

M

V

K

R

A

T

A

C

E

Q

K

E

Y

H

G

G

V

V

V

I

T

F

Q

M

M

A

G

G

N

H

Q
x
V

G

-

H

-

S

-

E

-

A

M

N

N

Y

F

F

F

Q

H

-

G

-

V

-

R

-

Y

F

A

K

K

A

K

W

L

V

I

D

N

A

D

G

G

I

V

I

I

K

G

D

K

V

V

T

-

A

-

I

L

T

Y

C

C

H

H

M

-

N

S

G

A

R

R

R

N

R

G

W

W

binding nicotinamide-adenine-dinucleotide: G20 (= G70), Y21 (≠ N71), F22 (≠ R72), L42 (≠ C92), D43 (= D93), P44 (≠ V94), A77 (= A134), T78 (= T135), P79 (= P136), N80 (≠ D137), H83 (= H140), E100 (= E157), K101 (= K158), V129 (≠ Q186)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 170, 171, 183, 308, 309

3nt5A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor and product inosose (see paper)
27% identity, 32% coverage: 62:216/492 of query aligns to 3:157/337 of 3nt5A

query
sites
3nt5A

V

L

N

R

L

I

A

G

C

V

V

I

G
|
G

I

T

G
|
G

N
x
A

R
x
I

G

G

E

K

Q

E

V

H

I

I

N

N

A

R

I

I

-

T

D

N

E

K

T

L

G

S

Q

G

A

A

N

E

I

I

V

V

T

A

L

V

C

T

D
|
D

V
|
V

D

N

M

Q

G

E

A

A

E

A

H

Q

T

K

L

V

P

V

S

E

M

Q

N

Y

K

Q

F

L

P

N

K

A

A

T

T

V

R

Y

F

P

Q

N

D

D

F

D

R

S

E

L

M

L

L

A

D

D

K

-

D

-

S

-

Q

E

H

N

F

V

D

D

A

A

V

V

M

L

V

V

A

T

T
x
S

P
x
W

D
x
G

F

P

S

A

H

H

F

E

P

S

V

S

T

V

M

L

A

K

A

A

M

I

A

K

A

A

G

Q

K

K

G

Y

V

V

Y

F

V

C

E
|
E

K
|
K

P

P

L

L

A

A

R

T

T

T

F

A

H

E

E

G

I

C

E

M

L

R

L

I

M

V

K

E

A

E

A

E

E

I

K

K

Y

V

G

G

-

K

V

R

V

L

T

V

Q

Q

M

V

G

G

N

F

Q

M

G

R

H

R

S

Y

E

D

A

S

N

G

Y

Y

F

V

Q

Q

F

L

K

K

A

E

W

A

V

L

D

D

A

N

G

H

I

V

I

I

K

G

D

E

V

P

T

L

A

M

I

I

T

H

C

C

-

A

H
|
H

M

R

N

N

active site: K97 (= K158)
binding (2R,3S,4s,5R,6S)-2,3,4,5,6-pentahydroxycyclohexanone: K97 (= K158), H155 (= H214)
binding nicotinamide-adenine-dinucleotide: G9 (= G68), G11 (= G70), A12 (≠ N71), I13 (≠ R72), D35 (= D93), V36 (= V94), S74 (≠ T135), W75 (≠ P136), G76 (≠ D137), E96 (= E157), K97 (= K158)

Sites not aligning to the query:

3nt4A Crystal structure of myo-inositol dehydrogenase from bacillus subtilis with bound cofactor nadh and inositol (see paper)
27% identity, 32% coverage: 62:216/492 of query aligns to 3:157/337 of 3nt4A

query
sites
3nt4A

V

L

N

R

L

I

A

G

C

V

V

I

G

G

I

T

G
|
G

N
x
A

R
x
I

G

G

E

K

Q

E

V

H

I

I

N

N

A

R

I

I

-

T

D

N

E

K

T

L

G

S

Q

G

A

A

N

E

I

I

V

V

T

A

L

V

C

T

D
|
D

V
|
V

D

N

M

Q

G

E

A

A

E

A

H

Q

T

K

L

V

P

V

S

E

M

Q

N

Y

K

Q

F

L

P

N

K

A

A

T

T

V

R

Y

F

P

Q

N

D

D

F

D

R

S

E

L

M

L

L

A

D

D

K

-

D

-

S

-

Q

E

H

N

F

V

D

D

A

A

V

V

M

L

V

V

A

T

T
x
S

P
x
W

D
x
G

F

P

S

A

H

H

F

E

P

S

V

S

T

V

M

L

A

K

A

A

M

I

A

K

A

A

G

Q

K

K

G

Y

V

V

Y

F

V

C

E
|
E

K
|
K

P

P

L

L

A

A

R

T

T

T

F

A

H

E

E

G

I

C

E

M

L

R

L

I

M

V

K

E

A

E

A

E

E

I

K

K

Y

V

G

G

-

K

V

R

V

L

T

V

Q

Q

M

V

G

G

N

F

Q

M

G

R

H

R

S

Y

E

D

A

S

N

G

Y

Y

F

V

Q

Q

F

L

K

K

A

E

W

A

V

L

D

D

A

N

G

H

I

V

I

I

K

G

D

E

V

P

T

L

A

M

I

I

T

H

C

C

-

A

H
|
H

M

R

N

N

active site: K97 (= K158)
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: H155 (= H214)
binding 1,4-dihydronicotinamide adenine dinucleotide: G11 (= G70), A12 (≠ N71), I13 (≠ R72), D35 (= D93), V36 (= V94), S74 (≠ T135), W75 (≠ P136), G76 (≠ D137), E96 (= E157), K97 (= K158)

Sites not aligning to the query:

active site: 176
binding 1,2,3,4,5,6-hexahydroxy-cyclohexane: 176, 235, 272
binding 1,4-dihydronicotinamide adenine dinucleotide: 176

Query Sequence

>Echvi_2865 FitnessBrowser__Cola:Echvi_2865
MKTEKNKVLQKAGERRDFLKKSALALGAVSIVPSHVLFSKPEIRDKQGKLLRKASFVPSD
RVNLACVGIGNRGEQVINAIDETGQANIVTLCDVDMGAEHTLPSMNKFPKATRFQDFREM
LDKDSQHFDAVMVATPDFSHFPVTMAAMAAGKGVYVEKPLARTFHEIELLMKAAEKYGVV
TQMGNQGHSEANYFQFKAWVDAGIIKDVTAITCHMNGRRRWHGWDVNMGKFPAAETIPDT
LDWDTWLTTAQHHDYNHDFINGQWRCWYDFGMGALGDWGAHIMDTFHQFLDLGLPYEVDP
VKIEGHNALFYPQATTLDFKFPKRGKMPEVNVSWYDGLDNIPEVPEGYGSSELDADIPAA
SNGKIQPAKLNPGKIIYGKELTFKGGSHGSTLSIIPDEKAKAMEGKLPEVPESPSNHFAN
FLLACKGEEKTRSPFSIAGPLSQVFTLGTLAQRLNAKLEFDRDKKVITNNKFANALLVGP
PPRKGWETYYKV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory