SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_2909 FitnessBrowser__Cola:Echvi_2909 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
47% identity, 100% coverage: 1:218/218 of query aligns to 3:219/223 of 2pclA

query
sites
2pclA

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binding magnesium ion: E165 (= E163), P166 (= P164), T167 (≠ S165), T196 (= T195)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
47% identity, 93% coverage: 16:218/218 of query aligns to 24:226/233 of P75957

query
sites
P75957

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G42 (= G34) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
47% identity, 93% coverage: 16:218/218 of query aligns to 21:223/226 of 7mdyC

query
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7mdyC

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binding adp orthovanadate: V21 (= V16), S40 (≠ A35), S41 (= S36), G42 (= G37), S43 (≠ A38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42), Q91 (= Q86), R135 (= R130), H138 (= H133), E142 (= E137), S144 (= S139), G145 (= G140), G146 (= G141), E147 (= E142), E168 (= E163), N172 (= N167), H201 (= H196)
binding magnesium ion: S46 (= S41), Q91 (= Q86)

Sites not aligning to the query:

binding adp orthovanadate: 12, 19

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs
47% identity, 93% coverage: 16:218/218 of query aligns to 23:225/229 of 7v8iD

query
sites
7v8iD

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binding phosphoaminophosphonic acid-adenylate ester: V23 (= V16), S43 (= S36), G44 (= G37), S45 (≠ A38), G46 (= G39), K47 (= K40), S48 (= S41), T49 (= T42), Q93 (= Q86), R137 (= R130), H140 (= H133), E144 (= E137), L145 (= L138), S146 (= S139), G147 (= G140), G148 (= G141), E149 (= E142), N174 (= N167), H203 (= H196)
binding magnesium ion: S48 (= S41), Q93 (= Q86)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
47% identity, 93% coverage: 16:217/218 of query aligns to 21:222/222 of 7arlD

query
sites
7arlD

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binding adenosine-5'-diphosphate: G42 (= G37), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
45% identity, 99% coverage: 1:216/218 of query aligns to 4:222/648 of P75831

query
sites
P75831

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S

S

A

G

E

E

S

E

L

V

H

M

E

A

L

I

F

L

F

H

T

Q

L

L

R

R

D

D

R

R

F

-

G

G

Q

H

S

T

F

V

V

I

I

I

V

V

T

T

H

H

N

D

Q

P

Q

Q

L

V

A

A

Q

A

M

Q

A

A

D

E

R

R

M

V

L

I

T

E

M

I

Q

R

D

D

G

G

V

E

I

I

V

V

K47 (= K40) mutation to L: Lack of activity.
D169 (= D162) mutation to N: Lack of activity.

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
41% identity, 100% coverage: 1:218/218 of query aligns to 3:223/615 of 5lilA

query
sites
5lilA

M

I

L

I

R

E

A

I

K

K

G

Q

I

L

H

N

K

R

Y

Y

Y
x
F

G

G

D

E

-

G

-

E

-

N

-

R

L

V

H

H

V
|
V

L

L

K

K

G

D

V

I

D

S

V

L

E

S

I

I

A

E

A

R

G

G

E

D

I

F

V

V

S

A

I

I

V

M

G

G

A

Q

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

H

N

I

I

L

I

G

G

T

C

L

L

D

D

D

T

A

A

D

T

K

G

G

G

L

S

V

S

S

K

I

I

D

D

D

G

K

K

S

E

L

T

T

I

A

E

L

L

K

T

G

N

D

D

K

Q

L

L

A

S

A

D

Y

L

R

R

N

S

Q

Q

E

K

V

F

G

G

F

F

I

I

F

F

Q
|
Q

F

R

H

Y

N

N

L

L

P

S

E

S

F

L

T

T

A

A

E

A

E

E

N

N

I

V

I

A

I

L

P

P

G

A

L

I

I

Y

A

A

K

G

K

M

D

P

E

Q

K

S

Y

Q

L

R

T

L

E

E

K

R

A

A

K

K

E

Q

L

L

A

L

R

E

L

K

L

L

G

G

I

L

M

G

D

D

R
x
K

L

W

G

Q

H
x
N

K

K

P

P

S

N

E
x
Q

L
|
L

S
|
S

G
|
G

E
x
Q

Q

Q

R

R

V

V

A

S

V

I

A

A

R

R

A

A

L

L

I

M

N

N

D

G

P

G

K

E

I

I

F

L

A

A

D
|
D

E
x
Q

P

P

S

T

G

G

N
x
A

L

L

D

D

T

S

Q

H

S

S

A

G

E

E

S

N

L

V

H

M

E

E

L

I

F

L

F

R

T

Q

L

L

R

H

D

E

R

E

F

-

G

G

Q

H

S

T

F

I

V

I

I

M

V

V

T

T

H
|
H

N

D

Q

K

Q

H

L

I

A

A

Q

A

M

S

A

A

D

N

R

R

M

I

L

I

T

E

M

I

Q

K

D

D

G

G

V

E

I

I

V

I

S

S

E

D

binding adenosine-5'-triphosphate: F13 (≠ Y11), V22 (= V16), S42 (= S36), G43 (= G37), S44 (≠ A38), G45 (= G39), K46 (= K40), S47 (= S41), T48 (= T42), Q92 (= Q86), K136 (≠ R130), N139 (≠ H133), Q143 (≠ E137), L144 (= L138), S145 (= S139), G146 (= G140), G147 (= G141), Q148 (≠ E142), Q169 (≠ E163), A173 (≠ N167), H201 (= H196)
binding magnesium ion: S47 (= S41), Q92 (= Q86), D168 (= D162)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
41% identity, 100% coverage: 1:218/218 of query aligns to 3:223/592 of 5lj7A

query
sites
5lj7A

M

I

L

I

R

E

A

I

K

K

G

Q

I

L

H

N

K

R

Y

Y

Y
x
F

G

G

D

E

-

G

-

E

-

N

-

R

L

V

H

H

V
|
V

L

L

K

K

G

D

V

I

D

S

V

L

E

S

I

I

A

E

A

R

G

G

E

D

I

F

V

V

S

A

I

I

V

M

G

G

A

Q

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

H

N

I

I

L

I

G

G

T

C

L

L

D

D

D

T

A

A

D

T

K

G

G

G

L

S

V

S

S

K

I

I

D

D

D

G

K

K

S

E

L

T

T

I

A

E

L

L

K

T

G

N

D

D

K

Q

L

L

A

S

A

D

Y

L

R

R

N

S

Q

Q

E

K

V

F

G

G

F

F

I

I

F

F

Q
|
Q

F

R

H

Y

N

N

L

L

P

S

E

S

F

L

T

T

A

A

E

A

E

E

N

N

I

V

I

A

I

L

P

P

G

A

L

I

I

Y

A

A

K

G

K

M

D

P

E

Q

K

S

Y

Q

L

R

T

L

E

E

K

R

A

A

K

K

E

Q

L

L

A

L

R

E

L

K

L

L

G

G

I

L

M

G

D

D

R
x
K

L

W

G

Q

H

N

K

K

P

P

S
x
N

E
x
Q

L
|
L

S
|
S

G
|
G

E
x
Q

Q

Q

R

R

V

V

A

S

V

I

A

A

R

R

A

A

L

L

I

M

N

N

D

G

P

G

K

E

I

I

F

L

A

A

D
|
D

E
x
Q

P

P

S

T

G

G

N
x
A

L

L

D

D

T

S

Q

H

S

S

A

G

E

E

S

N

L

V

H

M

E

E

L

I

F

L

F

R

T

Q

L

L

R

H

D

E

R

E

F

-

G

G

Q

H

S

T

F

I

V

I

I

M

V

V

T

T

H
|
H

N

D

Q

K

Q

H

L

I

A

A

Q

A

M

S

A

A

D

N

R

R

M

I

L

I

T

E

M

I

Q

K

D

D

G

G

V

E

I

I

V

I

S

S

E

D

binding adenosine-5'-triphosphate: F13 (≠ Y11), V22 (= V16), S42 (= S36), G43 (= G37), S44 (≠ A38), G45 (= G39), K46 (= K40), S47 (= S41), T48 (= T42), Q92 (= Q86), K136 (≠ R130), N142 (≠ S136), Q143 (≠ E137), L144 (= L138), S145 (= S139), G146 (= G140), G147 (= G141), Q148 (≠ E142), Q169 (≠ E163), A173 (≠ N167), H201 (= H196)
binding magnesium ion: S47 (= S41), Q92 (= Q86), D168 (= D162)

8g4cB Bacitracin export ATP-binding protein BceA (see paper)
41% identity, 100% coverage: 1:218/218 of query aligns to 3:224/248 of 8g4cB

query
sites
8g4cB

M

I

L

L

R

E

A

A

K

N

G

K

I

I

H

R

K

K

Y

S

Y
|
Y

G

G

D

N

-

K

-

L

-

N

-

K

L
x
Q

H

E

V

V

L

L

K

K

G

G

V

I

D

D

V

I

E

H

I

I

A

E

A

K

G

G

E

E

I

F

V

V

S

S

I

I

V

M

G

G

A

A

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
x
T

T
|
T

L

L

H

N

I

V

L

L

G

S

T

S

L

I

D

D

D

Q

A

V

D

S

K

H

G

G

L

T

V

I

S

H

I

I

D

N

D

G

K

N

S

D

L

M

T

T

A

A

L

M

K

K

G

E

D

K

K

Q

L

L

A

A

A

E

Y

F

R

R

N

K

Q

Q

E

H

V

L

G

G

F

F

I

I

F

F

Q

Q

F

D

H

Y

N

N

L

L

P

D

E

T

F

L

T

T

A

V

E

K

E

E

N

N

I

I

I

L

P

P

G

L

L

S

I

I

A

T

K

K

K

L

D

S

E

K

K

K

Y

E

L

A

T

N

E

R

K

K

A

F

K

E

E

E

L

V

A

A

R

K

L

E

L

L

G

G

I

I

M

Y

D

E

R

L

L

R

G

D

H

K

K

Y

P

P

S

N

E

E

L

I

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

T

A

S

V

A

A

G

R

R

A

A

L

F

I

I

N

H

D

D

P

P

K

S

I

I

F

F

A

A

D
|
D

E

E

P

P

S

T

G

G

N

A

L

L

D

D

T

S

Q

K

S

S

A

A

E

S

S

D

L

L

H

L

E

N

L

K

F

L

F

S

T

Q

L

L

R

N

D

Q

R

K

F

R

G

N

Q

A

S

T

F

I

V

I

I

M

V

V

T

T

H

H

N

D

Q

P

Q

V

L

A

A

A

Q

S

M

Y

A

C

D

G

R

R

M

V

L

I

T

F

M

I

Q

K

D

D

G

G

V

Q

I

M

V

Y

S

T

E

Q

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y11), Q20 (≠ L14), S42 (= S36), G43 (= G37), S44 (≠ A38), G45 (= G39), K46 (= K40), T47 (≠ S41), T48 (= T42), D168 (= D162)

7tchB Bceab e169q variant atp-bound conformation (see paper)
41% identity, 100% coverage: 1:218/218 of query aligns to 2:223/245 of 7tchB

query
sites
7tchB

M

I

L

L

R

E

A

A

K

N

G

K

I

I

H

R

K

K

Y

S

Y
|
Y

G

G

D

N

-

K

-

L

-

N

-

K

L
x
Q

H

E

V
|
V

L

L

K

K

G

G

V

I

D

D

V

I

E

H

I

I

A

E

A

K

G

G

E

E

I

F

V

V

S

S

I

I

V

M

G

G

A

A

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
x
T

T
|
T

L

L

H

N

I

V

L

L

G

S

T

S

L

I

D

D

D

Q

A

V

D

S

K

H

G

G

L

T

V

I

S

H

I

I

D

N

D

G

K

N

S

D

L

M

T

T

A

A

L

M

K

K

G

E

D

K

K

Q

L

L

A

A

A

E

Y

F

R

R

N

K

Q

Q

E

H

V

L

G

G

F

F

I

I

F

F

Q
|
Q

F

D

H

Y

N

N

L

L

P

D

E

T

F

L

T

T

A

V

E

K

E

E

N

N

I

I

I

L

P

P

G

L

L

S

I

I

A

T

K

K

K

L

D

S

E

K

K

K

Y

E

L

A

T

N

E

R

K

K

A

F

K

E

E

E

L

V

A

A

R

K

L

E

L

L

G

G

I

I

M

Y

D

E

R

L

L

R

G

D

H

K

K

Y

P

P

S

N

E
|
E

L

I

S
|
S

G
|
G

E

Q

Q

K

Q

Q

R

R

V

T

A

S

V

A

A

G

R

R

A

A

L

F

I

I

N

H

D

D

P

P

K

S

I

I

F

F

A

A

D

D

E

Q

P

P

S

T

G

G

N

A

L

L

D

D

T

S

Q

K

S

S

A

A

E

S

S

D

L

L

H

L

E

N

L

K

F

L

F

S

T

Q

L

L

R

N

D

Q

R

K

F

R

G

N

Q

A

S

T

F

I

V

I

I

M

V

V

T

T

H
|
H

N

D

Q

P

Q

V

L

A

A

A

Q

S

M

Y

A

C

D

G

R

R

M

V

L

I

T

F

M

I

Q

K

D

D

G

G

V

Q

I

M

V

Y

S

T

E

Q

binding adenosine-5'-triphosphate: Y12 (= Y11), Q19 (≠ L14), V21 (= V16), S41 (= S36), G42 (= G37), S43 (≠ A38), G44 (= G39), K45 (= K40), T46 (≠ S41), T47 (= T42), Q91 (= Q86), E142 (= E137), S144 (= S139), G145 (= G140), G146 (= G141), H201 (= H196)

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
40% identity, 100% coverage: 1:218/218 of query aligns to 1:226/232 of 1f3oA

query
sites
1f3oA

M

M

L

I

R

K

A

L

K

K

G

N

I

V

H

T

K

K

Y

T

Y
|
Y

-

K

-

M

G

G

D

E

-

E

-

I

L

I

H

Y

V
x
A

L

L

K

K

G

N

V

V

D

N

V

L

E

N

I

I

A

K

A

E

G

G

E

E

I

F

V

V

S

S

I

I

V

M

G

G

A

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

H

N

I

I

L

I

G

G

T

C

L

L

D

D

D

K

A

P

D

T

K

E

G

G

L

E

V

V

S

Y

I

I

D

D

D

N

K

I

S

K

L

T

T

N

A

D

L

L

K

D

G

D

D

D

K

E

L

L

A

T

A

K

Y

I

R

R

N

R

Q

D

E

K

V

I

G

G

F

F

I

V

F

F

Q

Q

F

Q

H

F

N

N

L

L

L

I

P

P

E

L

F

L

T

T

A

A

E

L

E

E

N

N

I

V

I

E

I

L

P

P

G

L

L

I

I

F

A

K

K

Y

K

R

D

G

E

A

K

M

Y

S

L

G

T

E

E

E

K

R

A

R

K

K

-

R

-

A

-

L

E

E

L

C

A

L

R

K

L

M

L

A

G

E

I

L

M

E

D

E

R

R

L

F

G

A

-

N

H

H

K

K

P

P

S

N

E

Q

L

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

I

A

N

N

D

N

P

P

K

P

I

I

F

L

A

A

D

D

E

E

P

P

S

T

G

G

N

A

L

L

D

D

T

S

Q

K

S

T

A

G

E

E

S

K

L

I

H

M

E

Q

L

L

F

L

F

K

T

K

L

L

R

N

D

E

R

E

F

D

G

G

Q

K

S

T

F

V

V

V

I

V

V

V

T

T

H

H

N

D

Q

I

Q

N

L

V

A

A

Q

R

M

F

A

G

D

E

R

R

M

I

L

I

T

Y

M

L

Q

K

D

D

G

G

V

E

I

V

V

E

S

R

E

E

binding adenosine-5'-diphosphate: Y11 (= Y11), A20 (≠ V16), S40 (= S36), G41 (= G37), S42 (≠ A38), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
39% identity, 100% coverage: 1:218/218 of query aligns to 1:226/230 of 1l2tA

query
sites
1l2tA

M

M

L

I

R

K

A

L

K

K

G

N

I

V

H

T

K

K

Y

T

Y
|
Y

-

K

-
x
M

G

G

D

E

-

E

-

I

L
x
I

H

Y

V
x
A

L

L

K

K

G

N

V

V

D

N

V

L

E

N

I

I

A

K

A

E

G

G

E

E

I

F

V

V

S

S

I

I

V

M

G

G

A
x
P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

M

L

L

H

N

I

I

L

I

G

G

T

C

L

L

D

D

D

K

A

P

D

T

K

E

G

G

L

E

V

V

S

Y

I

I

D

D

D

N

K

I

S

K

L

T

T

N

A

D

L

L

K

D

G

D

D

D

K

E

L

L

A

T

A

K

Y

I

R

R

N

R

Q

D

E

K

V

I

G

G

F

F

I

V

F

F

Q

Q

F

Q

H

F

N

N

L

L

L

I

P

P

E

L

F

L

T

T

A

A

E

L

E

E

N

N

I

V

I

E

I

L

P

P

G

L

L

I

I

F

A

K

K

Y

K

R

D

G

E

A

K

M

Y

S

L

G

T

E

E

E

K

R

A

R

K

K

-

R

-

A

-

L

E

E

L

C

A

L

R

K

L

M

L

A

G

E

I

L

M

E

D

E

R

R

L
x
F

G

A

-

N

H
|
H

K

K

P

P

S

N

E
x
Q

L

L

S
|
S

G
|
G

E
x
Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

I

A

N

N

D

N

P

P

K

P

I

I

F

L

A

A

D

D

E

Q

P

P

S

T

G

G

N
x
A

L

L

D

D

T

S

Q

K

S

T

A

G

E

E

S

K

L

I

H

M

E

Q

L

L

F

L

F

K

T

K

L

L

R

N

D

E

R

E

F

D

G

G

Q

K

S

T

F

V

V

V

I

V

V

V

T

T

H
|
H

N

D

Q

I

Q

N

L

V

A

A

Q

R

M

F

A

G

D

E

R

R

M

I

L

I

T

Y

M

L

Q

K

D

D

G

G

V

E

I

V

V

E

S

R

E

E

binding adenosine-5'-triphosphate: Y11 (= Y11), M13 (vs. gap), I18 (≠ L14), A20 (≠ V16), P39 (≠ A35), S40 (= S36), G41 (= G37), S42 (≠ A38), G43 (= G39), K44 (= K40), S45 (= S41), T46 (= T42), F138 (≠ L131), H141 (= H133), Q145 (≠ E137), S147 (= S139), G148 (= G140), G149 (= G141), Q150 (≠ E142), A175 (≠ N167), H204 (= H196)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
39% identity, 100% coverage: 1:218/218 of query aligns to 3:223/226 of 5xu1B

query
sites
5xu1B

M

L

L

I

R

S

A

L

K

K

G

N

I

I

H

F

K

R

Y

S

Y

Y

-

R

-

N

G

G

D

D

-

Q

-

E

L

L

H

Q

V

V

L

L

K

K

G

N

V

I

D

N

V

L

E

E

I

V

A

N

A

E

G

G

E

E

I

F

V

V

S

A

I

I

V

M

G

G

A

P

S

S

G

G

A

S

G
|
G

K
|
K

S
|
S

T

T

L

L

L

M

H

N

I

T

L

I

G

G

T

M

L

L

D

D

D

T

A

P

D

T

K

S

G

G

L

E

V

Y

S

Y

I

L

D

E

D

G

K

Q

S

E

L

V

T

A

A

G

L

L

K

G

G

E

D

K

K

Q

L

L

A

A

A

K

Y

V

R

R

N

N

Q

Q

E

Q

V

I

G

G

F

F

I

V

F

F

Q

Q

F

Q

H

F

N

F

L

L

P

S

E

K

F

L

T

N

A

A

E

L

E

Q

N

N

I

V

I

E

I

L

P

P

G

L

L

I

I

Y

A

A

K

G

K

V

D

S

E

S

K

S

Y

K

L

R

T

R

E

K

K

L

A

A

K

E

E

E

L

Y

A

L

R

D

L

K

L

V

G

E

I

L

M

T

D

E

R

R

L

S

G

H

H

H

K

L

P

P

S

S

E

E

L

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

I

V

N

N

D

N

P

P

K

S

I

I

F

L

A

A

D

D

E

E

P

P

S

T

G

G

N

A

L

L

D

D

T

T

Q

K

S

T

A

G

E

N

S

Q

L

I

H

M

E

Q

L

L

F

L

F

V

T

D

L

L

R

-

D

N

R

K

F

E

G

G

Q

K

S

T

F

I

V

I

I

M

V

V

T

T

H

H

N

E

Q

P

Q

E

L

I

A

A

Q

A

M

Y

A

A

D

K

R

R

M

Q

L

I

T

V

M

I

Q

R

D

D

G

G

V

V

I

I

V

S

S

S

E

D

binding magnesium ion: G45 (= G39), K46 (= K40), S47 (= S41)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
40% identity, 94% coverage: 14:218/218 of query aligns to 21:224/650 of 5ws4A

query
sites
5ws4A

L

I

H

Q

V
x
I

L

L

K

K

G

G

V

I

D

D

V

L

E

T

I

I

A

Y

A

E

G

G

E

E

I

L

V

V

S

A

I

I

V

V

G

G

A

Q

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

H

N

I

I

L

L

G

G

T

C

L

L

D

D

D

R

A

P

D

T

K

S

G

G

L

S

V

Y

S

K

I

V

D

N

D

G

K

Q

S

E

L

T

T

G

A

K

L

L

K

E

G

P

D

D

K

Q

L

L

A

A

A

Q

Y

L

R

R

N

R

Q

E

E

Y

V

F

G

G

F

F

I

I

F

F

Q

Q

F

R

H

Y

N

H

L

L

P

G

E

D

F

L

T

S

A

A

E

E

E

G

N

N

I

V

I

E

I

V

P

P

G

A

L

V

I

Y

A

A

K

G

K

V

D

T

E

P

K

A

Y

D

L

R

T

K

E

Q

K

R

A

A

K

T

E

A

L

L

A

L

R

T

L

E

L

L

G

G

I

L

M

G

D

T

R

K

L

T

G

Q

H

N

K

R

P

P

S

S

E

Q

L

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

A

S

V

I

A

A

R

R

A

A

L

L

I

M

N

N

D

G

P

G

K

D

I

V

I

I

F

L

A

A

D

D

E

E

P

P

S

T

G

G

N

A

L

L

D

D

T

S

Q

H

S

S

A

G

E

V

S

E

L

V

H

M

E

R

L

I

F

L

F

R

T

E

L

L

R

-

D

N

R

A

F

A

G

G

Q

H

S

T

F

I

V

I

I

L

V

V

T

T

H

H

N

D

Q

M

Q

Q

L

V

A

A

Q

K

M

N

A

A

D

T

R

R

M

I

L

I

T

E

M

I

Q

S

D

D

G

G

V

E

I

I

V

I

S

S

E

D

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: I23 (≠ V16), S43 (= S36), G44 (= G37), S45 (≠ A38), G46 (= G39), K47 (= K40), S48 (= S41), T49 (= T42)

Sites not aligning to the query:

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: 14

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
41% identity, 100% coverage: 1:218/218 of query aligns to 2:217/241 of 4u00A

query
sites
4u00A

M

I

L

I

R

R

A

I

K

R

G

N

I

L

H

H

K

K

Y

W

Y
x
F

G

G

D

P

L
|
L

H

H

V
|
V

L

L

K

K

G

G

V

I

D

H

V

L

E

E

I

V

A

A

A

P

G

G

E

E

I

K

V

L

S

V

I

I

V

I

G

G

A

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

H

R

I

T

L

I

G

N

T

R

L

L

D

E

D

D

A

F

D

Q

K

E

G

G

L

E

V

V

S

V

I

V

D

D

D

G

K

L

S

S

L

-

T

-

A

-

L

V

K

K

G

D

D

D

K

R

L

A

A

L

A

R

Y

E

R

I

N

R

Q

R

E

E

V

V

G

G

F

M

I

V

F

F

Q

Q

F

Q

H

F

N

N

L

L

L

F

P

P

E

H

F

M

T

T

A

V

E

L

E

E

N

N

I

V

I

T

I

L

-

A

P

P

G

M

L

R

I

V

A

R

K

R

K

W

D

P

E

R

K

E

Y

K

L

A

T

E

E

K

K

K

A

A

K

L

E

E

L

L

A

L

R

E

L

R

L

V

G

G

I

I

M

L

D

D

R

Q

L

A

G

R

H

K

K

Y

P

P

S

A

E

Q

L

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

I

A

N

M

D

E

P

P

K

K

I

I

I

M

F

L

A

F

D

D

E

E

P

P

S

T

G

S

N

A

L

L

D

D

T

-

Q

-

S

-

A

P

E

E

S

M

L

V

H

G

E

E

L

V

F

L

F

D

T

V

L

M

R

R

D

D

-

L

-

A

R

Q

F

G

G

G

Q

M

S

T

F

M

V

V

I

V

V

V

T

T

H

H

N

E

Q

M

Q

G

L

F

A

A

-

R

Q

E

M

V

A

A

D

D

R

R

M

V

L

V

T

F

M

M

Q

D

D

G

G

G

V

Q

I

I

V

V

S

E

E

E

binding adenosine-5'-diphosphate: F12 (≠ Y11), L15 (= L14), V17 (= V16), S37 (= S36), G38 (= G37), S39 (≠ A38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (= T42)

P9WQK5 Uncharacterized ABC transporter ATP-binding protein Rv0073 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 93% coverage: 9:210/218 of query aligns to 12:215/330 of P9WQK5

query
sites
P9WQK5

K

E

Y

Y

G

S

D

G

L

G

H

Y

V

A

L

L

K

R

-

P

-

I

-

N

G

G

V

L

D

N

V

L

E

D

I

V

A

A

G

G

E

S

I

L

V

V

S

M

I

L

V

L

G

G

A

P

S

S

G

G

A

C

G

G

K

K

S

T

T

T

L

L

H

S

I

C

L

L

G

G

T

G

L

I

D

L

D

R

A

P

D

K

K

S

G

G

L

A

V

I

S
x
K

I

F

D

D

D

E

K

V

S

D

L

I

T

T

A

T

L

L

K

Q

G

G

D

A

K

E

L

L

A

A

A

N

Y

Y

R

R

N

R

Q

N

E

K

V

V

G

G

F

I

I

V

F

F

Q

Q

F

A

H

F

N

N

L

L

L

V

P

P

E

S

F

L

T

T

A

A

E

V

E

E

N

N

I

V

I

M

I

V

P

P

G

L

L

R

I

S

A

A

K

G

K

M

D

S

E

R

K

R

Y

A

L

S

T

R

E

R

K

R

A

A

K

E

E

E

L

L

A

L

R

A

L

R

L

V

G

N

I

L

M

A

D

E

R

R

L

M

G

N

H

H

K

R

P

P

S

G

E

D

L

L

S

S

G

G

E

Q

Q

Q

R

R

V

V

A

A

V

V

A

A

R

R

A

A

L

I

I

A

N

L

D

D

P

P

K

P

I

L

I

I

F

L

A

A

D

D

E

E

P

P

S

T

G

A

N

H

L

L

D

D

T

F

Q

I

S

Q

A

V

E

E

S

E

L

V

H

L

E

R

L

L

F

I

F

R

T

E

L

L

R

A

D

D

R

-

F

-

G

G

Q

E

S

R

F

V

V

V

I

V

V

V

-

A

T

T

H

H

N

D

Q

S

Q

R

L

M

A

L

Q

P

M

M

A

A

D

D

R

R

M

V

L

V

T

E

M

L

K65 (≠ S59) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
39% identity, 100% coverage: 1:218/218 of query aligns to 1:217/240 of 4ymuJ

query
sites
4ymuJ

M

M

L

I

R

F

A

V

K

N

G

D

I

V

H

Y

K

K

Y

N

Y
x
F

G

G

D

S

L
|
L

H

E

V
|
V

L

L

K

K

G

G

V

V

D

T

V

L

E

K

I

V

A

N

A

K

G

G

E

E

I

V

V

V

S

V

I

I

V

I

G

G

A

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

H

R

I

C

L

I

G

N

T

L

L

L

D
x
E

D

E

A

P

D

T

K

K

G

G

L

E

V

V

S

F

I

I

D

D

D

G

K

V

S

K

L

I

T

N

A

N

L

G

K

K

G

V

D

N

K

-

L

I

A

N

A

K

Y

V

R

R

N

-

Q

Q

E

K

V

V

G

G

F

M

I

V

F

F

Q

Q

F

H

H

F

N

N

L

L

L

F

P

P

E

H

F

L

T

T

A

A

E

I

E

E

N

N

I

I

I

T

I

L

-

A

P

P

G

V

L

K

I

V

A

K

K

K

K

M

D

N

E

K

K

K

Y

E

L

A

T

E

E

E

K

L

A

A

K

V

E

D

L

L

A

L

R

A

L

K

L

V

G

G

I

L

M

L

D

D

R

K

L

K

G

D

H

Q

K

Y

P

P

S

I

E

K

L

L

S

S

G

G

E

Q

Q

K

Q

Q

R

R

V

L

A

A

V

I

A

A

R

R

A

A

L

L

I

A

N

M

D

Q

P

P

K

E

I

V

I

M

F

L

A

F

D

D

E
|
E

P

P

S

T

G

S

N

A

L

L

D

D

T

P

Q

E

S

M

A

V

E

K

S

E

L

V

H

L

E

N

L

V

F

M

F

K

T

Q

L

L

R

A

D

N

R

E

F

-

G

G

Q

M

S

T

F

M

V

V

I

V

V

V

T

T

H
|
H

N

E

Q

M

Q

G

L

F

A

A

-

R

Q

E

M

V

A

G

D

D

R

R

M

V

L

I

T

F

M

M

Q

D

D

D

G

G

V

V

I

I

V

V

S

E

E

E

binding adenosine-5'-triphosphate: F11 (≠ Y11), L14 (= L14), V16 (= V16), S36 (= S36), G37 (= G37), S38 (≠ A38), G39 (= G39), K40 (= K40), S41 (= S41), T42 (= T42), E51 (≠ D51), E162 (= E163), H194 (= H196)
binding magnesium ion: S41 (= S41), E162 (= E163)

3c4jA Abc protein artp in complex with atp-gamma-s
40% identity, 100% coverage: 1:218/218 of query aligns to 3:219/242 of 3c4jA

query
sites
3c4jA

M

M

L

I

R

D

A

V

K

H

G

Q

I

L

H

K

K

K

Y

S

Y
x
F

G

G

D

S

L

L

H

E

V
|
V

L

L

K

K

G

G

V

I

D

N

V

V

E

H

I

I

A

R

A

E

G

G

E

E

I

V

V

V

S

V

I

V

V

I

G

G

A
x
P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

H

R

I

C

L

L

G

N

T

L

L

L

D

E

D

D

A

F

D

D

K

E

G

G

L

E

V

I

S

I

I

I

D

D

D

G

K

I

S

N

L

L

T

K

A

A

L

-

K

K

G

D

D

T

K

N

L

L

A

N

A

K

Y

V

R

R

N

-

Q

E

E

E

V

V

G

G

F

M

I

V

F

F

Q

Q

F

R

H

F

N

N

L

L

L

F

P

P

E

H

F

M

T

T

A

V

E

L

E

N

N

N

I

I

I

T

I

L

P

A

G

P

L

M

I

K

A

V

K

R

K

K

D

W

E

P

K

R

Y

E

L

K

T

A

E

E

-

A

K

K

A

A

K

M

E

E

L

L

A

L

R

D

L

K

L

V

G

G

I

L

M

K

D

D

R

K

L

A

G
x
H

H

A

K
x
Y

P

P

S

D

E

S

L

L

S

S

G

G

E

Q

Q

A

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

I

A

N

M

D

E

P

P

K

K

I

I

I

M

F

L

A

F

D

D

E
|
E

P

P

S

T

G

S

N

A

L

L

D

D

T

P

Q

E

S

M

A

V

E

G

S

E

L

V

H

L

E

S

L

V

F

M

F

K

T

Q

L

L

R

A

D

N

R

E

F

-

G

G

Q

M

S

T

F

M

V

V

I

V

V

V

T

T

H

H

N

E

Q

M

Q

G

L

F

A

A

-

R

Q

E

M

V

A

G

D

D

R

R

M

V

L

L

T

F

M

M

Q

D

D

G

G

G

V

Y

I

I

V

I

S

E

E

E

binding phosphothiophosphoric acid-adenylate ester: F13 (≠ Y11), V18 (= V16), P37 (≠ A35), S38 (= S36), G39 (= G37), S40 (≠ A38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), H133 (≠ G132), Y135 (≠ K134), E164 (= E163)

3c41J Abc protein artp in complex with amp-pnp/mg2+
40% identity, 100% coverage: 1:218/218 of query aligns to 3:219/242 of 3c41J

query
sites
3c41J

M

M

L

I

R

D

A

V

K

H

G

Q

I

L

H

K

K

K

Y

S

Y
x
F

G

G

D

S

L

L

H

E

V
|
V

L

L

K

K

G

G

V

I

D

N

V

V

E

H

I

I

A

R

A

E

G

G

E

E

I

V

V

V

S

V

I

V

V

I

G

G

A
x
P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

H
x
R

I

C

L

L

G

N

T

L

L

L

D

E

D

D

A

F

D

D

K

E

G

G

L

E

V

I

S

I

I

I

D

D

D

G

K

I

S

N

L

L

T

K

A

A

L

-

K

K

G

D

D

T

K

N

L

L

A

N

A

K

Y

V

R

R

N

-

Q

E

E

E

V

V

G

G

F

M

I

V

F

F

Q

Q

F

R

H

F

N

N

L

L

L

F

P

P

E

H

F

M

T

T

A

V

E

L

E

N

N

N

I

I

I

T

I

L

P

A

G

P

L

M

I

K

A

V

K

R

K

K

D

W

E

P

K

R

Y

E

L

K

T

A

E

E

-

A

K

K

A

A

K

M

E

E

L

L

A

L

R

D

L

K

L

V

G

G

I

L

M

K

D

D

R

K

L

A

G
x
H

H

A

K
x
Y

P

P

S

D

E

S

L

L

S

S

G

G

E

Q

Q

A

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

I

A

N

M

D

E

P

P

K

K

I

I

I

M

F

L

A

F

D
|
D

E

E

P

P

S

T

G

S

N

A

L

L

D

D

T

P

Q

E

S

M

A

V

E

G

S

E

L

V

H

L

E

S

L

V

F

M

F

K

T

Q

L

L

R

A

D

N

R

E

F

-

G

G

Q

M

S

T

F

M

V

V

I

V

V

V

T

T

H

H

N

E

Q

M

Q

G

L

F

A

A

-

R

Q

E

M

V

A

G

D

D

R

R

M

V

L

L

T

F

M

M

Q

D

D

G

G

G

V

Y

I

I

V

I

S

E

E

E

binding phosphoaminophosphonic acid-adenylate ester: F13 (≠ Y11), V18 (= V16), P37 (≠ A35), S38 (= S36), G39 (= G37), S40 (≠ A38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), R47 (≠ H45), H133 (≠ G132), Y135 (≠ K134)
binding magnesium ion: S43 (= S41), D163 (= D162)

2olkA Abc protein artp in complex with adp-beta-s
40% identity, 100% coverage: 1:218/218 of query aligns to 3:219/242 of 2olkA

query
sites
2olkA

M

M

L

I

R

D

A

V

K

H

G

Q

I

L

H

K

K

K

Y

S

Y
x
F

G

G

D

S

L
|
L

H

E

V
|
V

L

L

K

K

G

G

V

I

D

N

V

V

E

H

I

I

A

R

A

E

G

G

E

E

I

V

V

V

S

V

I

V

V

I

G

G

A

P

S
|
S

G
|
G

A
x
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

H

R

I

C

L

L

G

N

T

L

L

L

D

E

D

D

A

F

D

D

K

E

G

G

L

E

V

I

S

I

I

I

D

D

D

G

K

I

S

N

L

L

T

K

A

A

L

-

K

K

G

D

D

T

K

N

L

L

A

N

A

K

Y

V

R

R

N

-

Q

E

E

E

V

V

G

G

F

M

I

V

F

F

Q

Q

F

R

H

F

N

N

L

L

L

F

P

P

E

H

F

M

T

T

A

V

E

L

E

N

N

N

I

I

I

T

I

L

P

A

G

P

L

M

I

K

A

V

K

R

K

K

D

W

E

P

K

R

Y

E

L

K

T

A

E

E

-

A

K

K

A

A

K

M

E

E

L

L

A

L

R

D

L

K

L

V

G

G

I

L

M

K

D

D

R

K

L

A

G

H

H

A

K

Y

P

P

S

D

E

S

L

L

S

S

G

G

E

Q

Q

A

Q

Q

R

R

V

V

A

A

V

I

A

A

R

R

A

A

L

L

I

A

N

M

D

E

P

P

K

K

I

I

I

M

F

L

A

F

D

D

E

E

P

P

S

T

G

S

N

A

L

L

D

D

T

P

Q

E

S

M

A

V

E

G

S

E

L

V

H

L

E

S

L

V

F

M

F

K

T

Q

L

L

R

A

D
&nbs