SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_3167 Echvi_3167 ribose-phosphate pyrophosphokinase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q58761 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
28% identity, 95% coverage: 4:286/297 of query aligns to 2:280/284 of Q58761

query
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Q58761

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DNE 34:36 (≠ DGE 36:38) binding
D212 (= D218) binding
STGGT 216:220 (≠ START 222:226) binding

1u9zA Crystal structure of phosphoribosyl diphosphate synthase complexed with amp and ribose 5-phosphate (see paper)
27% identity, 95% coverage: 4:286/297 of query aligns to 2:270/274 of 1u9zA

query
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1u9zA

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binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R92 (= R94), Q93 (= Q95), F97 (= F99), H125 (= H127)
binding ribose-5-phosphate: D163 (= D168), D202 (= D218), I205 (= I221), S206 (= S222), T207 (= T223), G208 (≠ A224), T210 (= T226)

Q97Z86 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
32% identity, 81% coverage: 34:273/297 of query aligns to 32:271/291 of Q97Z86

query
sites
Q97Z86

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STGGT 220:224 (≠ START 222:226) binding

4twbA Sulfolobus solfataricus ribose-phosphate pyrophosphokinase (see paper)
31% identity, 81% coverage: 34:273/297 of query aligns to 32:258/278 of 4twbA

query
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4twbA

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binding adenosine monophosphate: F32 (= F34), D34 (= D36), E36 (= E38), R93 (= R94), F98 (= F99), H127 (= H127)

Q97CA5 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Thermoplasma volcanium (strain ATCC 51530 / DSM 4299 / JCM 9571 / NBRC 15438 / GSS1) (see paper)
27% identity, 89% coverage: 5:269/297 of query aligns to 3:261/286 of Q97CA5

query
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Q97CA5

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L

Y

G

S

E

K

V

V

Y

-

Q

-

I

-

P

K

N

F

K

S

V

D

I

L

H

H

A

A

A

N

D

D

A

A

I

I

S

V

D

R

W

Y

I

Y

K

K

E

-

N

N

I

V

E

D

N

V

P

D

V

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

S

A

E

D

V

I

A

S

K

A

N

K

A

L

G

G

A

K

P

K

F

H

I

F

V

F

L

I

Q

E

K

K

M

K

R
|
R

H

I

G

D

D

D

R

R

D

T

V

V

E

E

V

M

S

K

V

V

P

P

D

N

V

V

D

D

I

V

Y

-

K

N

D

G

A

K

T

K

P

L

I

L

L

I

V

V

D
|
D

D

D

I

I

S
|
S

T
|
T

A
x
G

R
x
G

T
|
T

M

I

I

A

E

K

T

S

V

S

Q

G

H

L

L

L

K

R

K

E

A

K

G

G

M

A

K

S

P

K

P

I

I

Y

C

V

V

S

G

A

I

V

H

H

A

G

V

L

F

F

S

V

G

N

N

G

S

S

Y

E

K

N

D

K

L

I

L

L

G

Q

S

N

G

A

V

D

E

E

K

I

I

H

V

V

T

T

DGE 34:36 (= DGE 36:38) binding
RQH 91:93 (≠ RQD 94:96) binding
R186 (= R193) binding
D210 (= D218) binding
STGGT 214:218 (≠ START 222:226) binding

3lpnA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with an atp analog (ampcpp). (see paper)
27% identity, 89% coverage: 5:269/297 of query aligns to 3:261/284 of 3lpnA

query
sites
3lpnA

L

I

F

I

S

A

L

L

P
x
R

G

S

N

S

E

L

K
|
K

L

L

T

A

E

A

L
x
R

M

I

A

A

T

E

K

E

M

L

D

K

A

T

E

E

V

P

G

V

K

M

A

P

T

D

L

E

R

R

N

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

Y

Y

T

L

R

R

I

Y

L

D

S

E

D

D

V

L

K

T

D

G

K

H

C

N

V

I

V

F

L

I

V

I

C

G

T

N

L

T

H

H

E

-

P
x
S

D

D

E

A

K

E

L

V

L

M

P

E

L

M

Y

I

F

L

L

T

S

L

H

S

T

A

A

I

K

Q

S

D

L

Y

G

R

A

T

M

K

C

S

T

V

C

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

K

Q

V

R

F
x
Y

N

K

E

N

G

G

E

E

G

P

V

I

T

S

S

S

G

Q

F

I

F

L

G

T

K

E

L

I

I

Y

S

S

G

S

F

Y

A

S

D

N

S

S

I

I

T

A

T

T

V

V

D

D

P

I

H
|
H

L
x
D

H

E

R

K

I

T

S

L

S

S

L

Y

G

S

E

K

V

V

Y

-

Q

-

I

-

P

K

N

F

K

S

V

D

I

L

H

H

A

A

A

N

D

D

A

A

I

I

S

V

D

R

W

Y

I

Y

K

K

E

-

N

N

I

V

E

D

N

V

P

D

V

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

S

A

E

D

V

I

A

S

K

A

N

K

A

L

G

G

A

K

P

K

F

H

I

F

V

F

L

I

Q

E

K

K

M

K

R

R

H

I

G

D

D

D

R

R

D

T

V

V

E

E

V

M

S

K

V

V

P

P

D

N

V

V

D

D

I

V

Y

-

K

N

D

G

A

K

T

K

P

L

I

L

L

I

V

V

D

D

I

I

S
|
S

T
|
T

A
x
G

R

G

T
|
T

M

I

I

A

E

K

T

S

V

S

Q

G

H

L

L

L

K

R

K

E

A

K

G

G

M

A

K

S

P

K

P

I

I

Y

C

V

V

S

G

A

I

V

H

H

A

G

V

L

F

F

S

V

G

N

N

G

S

S

Y

E

K

N

D

K

L

I

L

L

G

Q

S

N

G

A

V

D

E

E

K

I

I

H

V

V

T

T

binding diphosphomethylphosphonic acid adenosyl ester: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), H93 (≠ D96), Y96 (≠ F99), H124 (= H127), D125 (≠ L128)
binding sulfate ion: R7 (≠ P9), K11 (= K13), R15 (≠ L17), S58 (≠ P61), S214 (= S222), T215 (= T223), G216 (≠ A224), T218 (= T226)

3mbiD Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
27% identity, 89% coverage: 5:269/297 of query aligns to 3:261/285 of 3mbiD

query
sites
3mbiD

L

I

F

I

S

A

L

L

P

R

G

S

N

S

E

L

K

K

L

L

T

A

E

A

L

R

M

I

A

A

T

E

K

E

M

L

D

K

A

T

E

E

V

P

G

V

K

M

A

P

T

D

L

E

R

R

N

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

Y

Y

T

L

R

R

I

Y

L

D

S

E

D

D

V

L

K

T

D

G

K

H

C

N

V

I

V

F

L

I

V

I

C

G

T

N

L

T

H

H

E

-

P

S

D

D

E

A

K

E

L

V

L

M

P

E

L

M

Y

I

F

L

L

T

S

L

H

S

T

A

A

I

K

Q

S

D

L

Y

G

R

A

T

M

K

C

S

T

V

C

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D

H

K

Q

V

R

F
x
Y

N

K

E

N

G

G

E

E

G

P

V

I

T

S

S

S

G

Q

F

I

F

L

G

T

K

E

L

I

I

Y

S

S

G

S

F

Y

A

S

D

N

S

S

I

I

T

A

T

T

V

V

D

D

P

I

H
|
H

L

D

H

E

R

K

I

T

S

L

S

S

L

Y

G

S

E

K

V

V

Y

-

Q

-

I

-

P

K

N

F

K

S

V

D

I

L

H

H

A

A

A

N

D

D

A

A

I

I

S

V

D

R

W

Y

I

Y

K

K

E

-

N

N

I

V

E

D

N

V

P

D

V

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

S

A

E

D

V

I

A

S

K

A

N

K

A

L

G

G

A

K

P

K

F

H

I

F

V

F

L

I

Q

E

K
|
K

M

K

R
|
R

H

I

G

D

D

D

R

R

D

T

V

V

E

E

V

M

S

K

V

V

P

P

D

N

V

V

D

D

I

V

Y

-

K

N

D

G

A

K

T

K

P

L

I

L

L

I

V

V

D
|
D

I
|
I

I

I

S
|
S

T
|
T

A
x
G

R

G

T
|
T

M

I

I

A

E

K

T

S

V

S

Q

G

H

L

L

L

K

R

K

E

A

K

G

G

M

A

K

S

P

K

P

I

I

Y

C

V

V

S

G

A

I

V

H

H

A

G

V

L

F

F

S

V

G

N

N

G

S

S

Y

E

K

N

D

K

L

I

L

L

G

Q

S

N

G

A

V

D

E

E

K

I

I

H

V

V

T

T

binding adenosine-5'-diphosphate: F32 (= F34), D34 (= D36), E36 (= E38), R91 (= R94), Q92 (= Q95), Y96 (≠ F99), H124 (= H127), K184 (= K191), R186 (= R193)
binding 5-O-phosphono-alpha-D-ribofuranose: R91 (= R94), H124 (= H127), K184 (= K191), R186 (= R193), D210 (= D218), D211 (= D219), I212 (= I220), S214 (= S222), T215 (= T223), G216 (≠ A224), T218 (= T226)

3mbiA Crystal structure of the phosphoribosylpyrophosphate (prpp) synthetase from thermoplasma volcanium in complex with adp-mg2+ and ribose 5- phosphate (see paper)
27% identity, 89% coverage: 5:269/297 of query aligns to 5:263/287 of 3mbiA

query
sites
3mbiA

L

I

F

I

S

A

L

L

P

R

G

S

N

S

E

L

K

K

L

L

T

A

E

A

L

R

M

I

A

A

T

E

K

E

M

L

D

K

A

T

E

E

V

P

G

V

K

M

A

P

T

D

L

E

R

R

N

R

F
|
F

P

P

D
|
D

G

G

E
|
E

S

L

Y

Y

T

L

R

R

I

Y

L

D

S

E

D

D

V

L

K

T

D

G

K

H

C

N

V

I

V

F

L

I

V

I

C

G

T

N

L

T

H

H

E

-

P

S

D

D

E

A

K

E

L

V

L

M

P

E

L

M

Y

I

F

L

L

T

S

L

H

S

T

A

A

I

K

Q

S

D

L

Y

G

R

A

T

M

K

C

S

T

V

C

N

L

I

V

I

A

A

P

P

Y

Y

L

Y

A

G

Y

Y

M

A

R
|
R

Q
|
Q

D
x
H

K

Q

V

R

F
x
Y

N

K

E

N

G

G

E

E

G

P

V

I

T

S

S

S

G

Q

F

I

F

L

G

T

K

E

L

I

I

Y

S

S

G

S

F

Y

A

S

D

N

S

S

I

I

T

A

T

T

V

V

D

D

P

I

H
|
H

L

D

H

E

R

K

I

T

S

L

S

S

L

Y

G

S

E

K

V

V

Y

-

Q

-

I

-

P

K

N

F

K

S

V

D

I

L

H

H

A

A

A

N

D

D

A

A

I

I

S

V

D

R

W

Y

I

Y

K

K

E

-

N

N

I

V

E

D

N

V

P

D

V

Y

L

V

I

V

G

S

P

P

D

D

S

D

E

G

S

G

E

L

Q

A

W

R

V

V

S

A

E

D

V

I

A

S

K

A

N

K

A

L

G

G

A

K

P

K

F

H

I

F

V

F

L

I

Q

E

K
|
K

M

K

R
|
R

H

I

G

D

D

D

R

R

D

T

V

V

E

E

V

M

S

K

V

V

P

P

D

N

V

V

D

D

I

V

Y

-

K

N

D

G

A

K

T

K

P

L

I

L

L

I

V

V

D

D

I

I

S
|
S

T
|
T

A
x
G

R

G

T
|
T

M

I

I

A

E

K

T

S

V

S

Q

G

H

L

L

L

K

R

K

E

A

K

G

G

M

A

K

S

P

K

P

I

I

Y

C

V

V

S

G

A

I

V

H

H

A

G

V

L

F

F

S

V

G

N

N

G

S

S

Y

E

K

N

D

K

L

I

L

L

G

Q

S

N

G

A

V

D

E

E

K

I

I

H

V

V

T

T

binding adenosine-5'-diphosphate: F34 (= F34), D36 (= D36), E38 (= E38), R93 (= R94), Q94 (= Q95), Y98 (≠ F99), H126 (= H127), K186 (= K191), R188 (= R193)
binding magnesium ion: H95 (≠ D96), H126 (= H127)
binding phosphate ion: R188 (= R193), S216 (= S222), T217 (= T223), G218 (≠ A224), T220 (= T226)

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
27% identity, 97% coverage: 5:292/297 of query aligns to 4:294/305 of 2hcrA

query
sites
2hcrA

L

I

F

F

S

S

L

G

P

S

G

S

N

H

E

Q

K

D

L

L

T

S

E

Q

L

K

M

I

A

A

T

D

K

R

M

L

D

G

A

L

E

E

V

L

G

G

K

K

A

V

T

V

L

T

R

K

N

K

F

F

P

S

D

N

G

Q

E

E

S

T

Y

C

T

V

R

E

I

I

L

G

S

E

D

S

V

V

K

R

D

G

K

E

C

D

V

V

V

Y

L

I

V

V

C

Q

T

S

-

G

L

C

H

G

E

E

P

I

D

N

E

D

K

N

L

L

L

M

P

E

L

L

Y

L

F

I

L

M

S

I

H

N

T

A

A

C

K

K

S

I

L

A

G

S

A

A

M

S

C

R

T

V

C

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

K
|
K

V

K

F

D

N

K

E

S

G

R

E

A

G

P

V

I

T

S

S

A

G

K

F

L

F

V

G

A

K

N

L

M

I

L

S

S

-

V

G

A

F

G

A

A

D

D

S

H

I

I

T

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

Q

D

I

I

P

P

N

V

K

D

V

N

I

L

H

Y

A

A

A

E

D

P

A

A

I

V

S

L

D

K

W

W

I

I

K

R

E

E

N

N

I

I

-

S

-

E

-

W

E

R

N

N

P

C

V

T

L

I

I

V

G

S

P

P

D

D

S

A

E

G

S

G

E

A

Q

K

W

R

V

V

S

T

E

S

V

I

A

A

K

D

N

R

A

L

G

N

A

V

P

D

F

F

I

A

V

L

L

I

Q

-

K

-

M

-

R

-

H

H

G

K

D

E

R

R

D

D

V

R

E

M

V

V

S

L

V

V

P

G

D

D

V

V

D

-

I

-

Y

-

K

K

D

D

A

R

T

V

P

A

I

I

L

L

V

V

D

D

I

M

I

A

S

D

T

T

A

C

R

G

T

T

M

I

I

C

E

H

T

A

V

A

Q

D

H

K

L

L

K

L

K

S

A

A

G

G

M

A

K

T

P

R

P

V

I

Y

C

A

V

I

G

L

I

T

H

H

A

G

V

I

F

F

S

S

G

G

N

P

S

A

Y

I

K

S

D

R

L

I

L

N

G

N

S

A

G

C

V

F

E

E

K

A

I

V

V

V

T

V

C

T

N

N

T

T

I

I

P

P

H

Q

P

E

S

D

-

K

-

M

-

K

-

H

-

C

-

S

-

K

-

I

N

Q

G

V

I

I

D

D

L

I

S

S

D

M

I

I

M

L

A

A

K

E

E

A

V

I

K

R

binding adenosine monophosphate: R94 (= R94), Q95 (= Q95), K97 (= K97), H128 (= H127)

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
27% identity, 97% coverage: 5:292/297 of query aligns to 6:302/318 of P60891

query
sites
P60891

L

I

F

F

S

S

L

G

P

S

G

S

N

H

E

Q

K

D

L

L

T
x
S

E

Q

L

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M

I

A

A

T

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K

R

M

L

D

G

A

L

E

E

V

L

G

G

K

K

A

V

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V

L

T

R

K

N

K

F

F

P

S

D

N

G

Q

E

E

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Y

C

T

V

R

E

I

I

L

G

S

E

D

S

V

V

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R

D

G

K

E

C
x
D

V

V

V

Y

L

I

V

V

C

Q

T

S

-

G

L

C

H

G

E

E

P

I

D

N

E

D

K

N

L

L

L

M

P

E

L

L

Y

L

F

I

L

M

S

I

H

N

T

A

A

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A

A

M

S

C

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C

T

L

A

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V

A

I

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P

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C

L

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A

P

Y

Y

M

A

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R

Q

Q

D

D

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K

V

K

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N

K

E

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R

E

A

G

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S

A

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L

F

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x
N

L

M

I

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-

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G

A

A

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D

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x
L

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L

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x
S

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Q

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I

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G

L

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G

-

E

-

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-

Y

F

Q

D

I

I

P

P

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x
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x
N

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x
Y

A

A

A

E

D

P

A

A

I

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W

W

I

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E

E

N

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x
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x
A

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x
H

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x
D

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M

V

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L

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-

K

K

D

D

A

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P

A

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L

L

V
|
V

D

D

I

M

I

A

S

D

T

T

A

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R

G

T

T

M

I

I

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E
x
H

T

A

V

A

Q

D

H

K

L

L

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A

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M

A

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P

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C

A

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I

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I

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N

G

N

S

A

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C

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E

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A

I

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C

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N

N

T

T

I

I

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P

H

Q

P

E

S

D

N

K

G

M

-

K

-

H

-

C

-

S

-

K

-

I

-

Q

-

V

I

I

D

D

L

I

S

S

D

M

I

I

M

L

A

A

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E

E

A

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I

K

R

S16 (≠ T15) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (≠ C51) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ K112) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (≠ P126) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ H129) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ N142) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (≠ V144) mutation to H: No effect on catalytic activity.
Y146 (≠ H146) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ K180) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (≠ I187) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ Q190) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ E200) to H: in a breast cancer sample; somatic mutation
V219 (= V217) to G: in a breast cancer sample; somatic mutation
H231 (≠ E229) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
27% identity, 97% coverage: 5:292/297 of query aligns to 5:301/316 of 8dbkB

query
sites
8dbkB

L

I

F

F

S

S

L

G

P

S

G

S

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A

L

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E

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L

G

G

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A

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x
N

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E
|
E

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D
x
S

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x
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V

Y

L

I

V

V

C

Q

T

S

-

G

L

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G

E

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I

D

N

E

D

K

N

L

L

L

M

P

E

L

L

Y

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F

I

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M

S

I

H

N

T

A

A

C

K

K

S

I

L

A

G

S

A

A

M

S

C

R

T

V

C

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

K

K

V

K

F

D

N

K

E

S

G

R

E

A

G

P

V

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T

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S

A

G

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F

L

F

V

G

A

K

N

L

M

I

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-

V

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A

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G

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A

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|
H

L

A

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R

Q

I

I

S

Q

S

G

L

F

G

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E

-

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Y

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I

I

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N

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N

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A

A

A

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D

P

A

A

I

V

S

L

D

K

W

W

I

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E

N

N

I

I

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S

-

E

-

W

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D

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x
G

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G

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R

V

V

S

T

E

S

V

I

A

A

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R

A

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A

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F

I

A

V

L

L

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R

H

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K

D

A

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x
N

D

E

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V

E

D

V

R

S

M

V

V

P

L

D

V

V

G

D

D

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-

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K

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D

A

R

T

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P

A

I

I

L

L

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V

D
|
D

I

M

I

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x
D

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|
T

A
x
C

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x
G

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|
T

M

I

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E

H

T

A

V

A

Q

D

H

K

L

L

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A

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G

M

A

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V

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C

A

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H

A

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F

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S

G

G

N

P

S

A

Y

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R

L

I

L

N

G

N

S

A

G

C

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F

E

E

K

A

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V

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V

C

T

N

N

T

T

I

I

P

P

H

Q

P

E

S

D

-

K

-

M

-

K

-

H

-

C

-

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-

K

-

I

N

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V

I

I

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D

L

I

S

S

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M

I

I

M

L

A

A

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E

E

A

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K

R

binding adenosine monophosphate: R95 (= R94), Q96 (= Q95), N199 (≠ R197)
binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38)
binding phosphate ion: S46 (≠ D46), R48 (≠ K48)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H127), D170 (= D168), G172 (≠ E170), K193 (= K191), R195 (= R193), D219 (= D218), D220 (= D219), D223 (≠ S222), T224 (= T223), C225 (≠ A224), G226 (≠ R225), T227 (= T226)

8dbeA Human prps1 with adp; hexamer (see paper)
27% identity, 97% coverage: 5:292/297 of query aligns to 5:301/316 of 8dbeA

query
sites
8dbeA

L

I

F

F

S

S

L

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G

S

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D

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L

T

S

E

Q

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I

A

A

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K

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M

L

D

G

A

L

E

E

V

L

G

G

K

K

A

V

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V

L

T

R

K

N

K

F
|
F

P

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x
N

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E

S

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C

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R

E

I

I

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D

S

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G

K

E

C

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V

V

V

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L

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V

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C

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L

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H

G

E

E

P

I

D

N

E

D

K

N

L

L

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P

E

L

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L

F

I

L

M

S

I

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N

T

A

A

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I

L

A

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A

A

M

S

C

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V

C

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V

A

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C

L

F

A

P

Y

Y

M

A

R
|
R

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Q

D

D

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K

V
x
K

F
x
D

N

K

E
x
S

G
x
R

E

A

G

P

V

I

T

S

S

A

G

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F

L

F

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A

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N

L

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I

L

S

S

-

V

G

A

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G

A

A

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M

D

D

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|
H

L

A

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I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

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N

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K
x
D

V

N

I

L

H
x
Y

A

A

A

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D

P

A

A

I

V

S

L

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K

W

W

I

I

K

R

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E

N

N

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I

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N

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G

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I

A

A

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D

N

R

A

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N

A

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F

I

A

V

L

L

I

Q

H

K

K

M

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R

H

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G

K

D

A

R

N

D

E

V

V

E

D

V

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M

V

V

P

L

D

V

V

G

D

D

I

V

Y

-

K

K

D

D

A

R

T

V

P

A

I

I

L

L

V

V

D
|
D

I

M

I

A

S
x
D

T
|
T

A

C

R
x
G

T
|
T

M

I

I

C

E

H

T

A

V

A

Q

D

H

K

L

L

K

L

K

S

A

A

G

G

M

A

K

T

P

R

P

V

I

Y

C

A

V

I

G

L

I

T

H

H

A

G

V

I

F

F

S

S

G

G

N

P

S

A

Y

I

K

S

D

R

L

I

L

N

G

N

S

A

G

C

V

F

E

E

K

A

I

V

V

V

T

V

C

T

N

N

T

T

I

I

P

P

H

Q

P

E

S

D

-

K

-

M

-

K

-

H

-

C

-

S

-

K

-

I

N

Q

G

V

I

I

D

D

L

I

S

S

D

M

I

I

M

L

A

A

K

E

E

A

V

I

K

R

binding adenosine-5'-diphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R94), Q96 (= Q95), K98 (= K97), K99 (≠ V98), D100 (≠ F99), S102 (≠ E101), R103 (≠ G102), H129 (= H127), D142 (≠ K143), Y145 (≠ H146)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H127), D170 (= D168), D219 (= D218), D220 (= D219), D223 (≠ S222), T224 (= T223), G226 (≠ R225), T227 (= T226)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 307, 308, 309, 312

7yk1A Structural basis of human prps2 filaments (see paper)
26% identity, 97% coverage: 4:292/297 of query aligns to 4:292/306 of 7yk1A

query
sites
7yk1A

I

V

L

L

F

F

S

S

L

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A

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A

L

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E

V

L

G

G

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K

A

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K

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x
N

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E

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E

I

I

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G

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x
S

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x
R

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K

E

C

D

V

V

V

Y

L

I

V

I

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G

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G

E

E

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I

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N

E

D

K

N

L

L

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P

E

L

L

Y

L

F

I

L

M

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I

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N

T

A

A

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K

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A

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A

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C

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V

C

T

L

A

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V

A

I

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P

Y

C

L

F

A

P

Y

Y

M

A

R
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R

Q

Q

D

D

K

K

V
x
K

F
x
D

N
x
K

E
x
S

G
x
R

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A

G

P

V

I

T

S

S

A

G

K

F

L

F

V

G

A

K

N

L

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I

L

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V

G

A

F

G

A

A

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I

T

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M

D

D

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L

H
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H

L

A

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I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

Q

D

I

I

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N

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K
x
D

V

N

I

L

H

Y

A

A

A

E

D

P

A

A

I

V

S

L

D

Q

W

W

I

I

K

R

E

E

N

N

I

I

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A

-

E

-

W

E

K

N

N

P

C

V

I

L

I

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A

E

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S

G

E

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A

A

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N

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A

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G

N

A

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F

I

A

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L

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K

M

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R

M

H

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-

V

V

S

L

V

V

P

G

D

D

V

V

D

-

I

-

Y

-

K

K

D

D

A

R

T

V

P

A

I

I

L

L

V

V

D

D

I

M

I

A

S
x
D

T

T

A
x
C

R

G

T
|
T

M

I

I

C

E

H

T

A

V

A

Q

D

H

K

L

L

K

L

K

S

A

A

G

G

M

A

K

T

P

K

P

V

I

Y

C

A

V

I

G

L

I

T

H

H

A

G

V

I

F

F

S

S

G

G

N

P

S

A

Y

I

K

S

D

R

L

I

L

N

G

N

S

A

G

A

V

F

E

E

K

A

I

V

V

V

T

V

C

T

N

N

T

T

I

I

P

P

H

Q

P

E

S

D

-

K

-

M

-

K

-

H

-

C

-

T

-

K

-

I

N

Q

G

V

I

I

D

D

L

I

S

S

D

M

I

I

M

L

A

A

K

E

E

A

V

I

K

R

binding adenosine-5'-diphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), S46 (≠ D46), R48 (≠ K48), R95 (= R94), K99 (≠ V98), D100 (≠ F99), K101 (≠ N100), S102 (≠ E101), R103 (≠ G102), H129 (= H127), D142 (≠ K143)
binding phosphate ion: D214 (≠ S222), C216 (≠ A224), T218 (= T226)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 298, 300, 303

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
27% identity, 97% coverage: 5:292/297 of query aligns to 5:294/309 of 8dbgA

query
sites
8dbgA

L

I

F

F

S

S

L

G

P

S

G

S

N

H

E

Q

K

D

L

L

T

S

E

Q

L

K

M

I

A

A

T

D

K

R

M

L

D

G

A

L

E

E

V

L

G

G

K

K

A

V

T

V

L

T

R

K

N

K

F
|
F

P

S

D
x
N

G

Q

E
|
E

S

T

Y

C

T

V

R

E

I

I

L

G

S

E

D
x
S

V

V

K
x
R

D

G

K

E

C

D

V

V

V

Y

L

I

V

V

C

Q

T

S

-

G

L

C

H

G

E

E

P

I

D

N

E

D

K

N

L

L

L

M

P

E

L

L

Y

L

F

I

L

M

S

I

H

N

T

A

A

C

K

K

S

I

L

A

G

S

A

A

M

S

C

R

T

V

C

T

L

A

V

V

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R
|
R

Q
|
Q

D

D

K
|
K

V

K

F

D

N

K

E

S

G

R

E

A

G

P

V

I

T

S

S

A

G

K

F

L

F

V

G

A

K

N

L

M

I

L

S

S

-

V

G

A

F

G

A

A

D

D

S

H

I

I

T

I

T

T

V

M

D

D

P

L

H
|
H

L

A

H

S

R

Q

I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

Q

D

I

I

P

P

N

V

K

D

V

N

I

L

H

Y

A

A

A

E

D

P

A

A

I

V

S

L

D

K

W

W

I

I

K

R

E

E

N

N

I

I

-

S

-

E

-

W

E

R

N

N

P

C

V

T

L

I

I

V

G

S

P

P

D

D

S

A

E

G

S

G

E

A

Q

K

W

R

V

V

S

T

E

S

V

I

A

A

K

D

N

R

A

L

G

N

A

V

P

D

F

F

I

A

V

L

L

I

Q

H

K

K

M

-

R

-

H

-

G

-

D

-

R

E

D

D

V

R

E

M

V

V

S

L

V

V

P

G

D

D

V

V

D

-

I

-

Y

-

K

K

D

D

A

R

T

V

P

A

I

I

L

L

V

V

D

D

I

M

I

A

S
x
D

T
|
T

A
x
C

R

G

T
|
T

M

I

I

C

E

H

T

A

V

A

Q

D

H

K

L

L

K

L

K

S

A

A

G

G

M

A

K

T

P

R

P

V

I

Y

C

A

V

I

G

L

I

T

H

H

A

G

V

I

F

F

S

S

G

G

N

P

S

A

Y

I

K

S

D

R

L

I

L

N

G

N

S

A

G

C

V

F

E

E

K

A

I

V

V

V

T

V

C

T

N

N

T

T

I

I

P

P

H

Q

P

E

S

D

-

K

-

M

-

K

-

H

-

C

-

S

-

K

-

I

N

Q

G

V

I

I

D

D

L

I

S

S

D

M

I

I

M

L

A

A

K

E

E

A

V

I

K

R

binding adenosine-5'-triphosphate: F34 (= F34), N36 (≠ D36), E38 (= E38), R95 (= R94), Q96 (= Q95), K98 (= K97), H129 (= H127)
binding phosphate ion: S46 (≠ D46), R48 (≠ K48), D216 (≠ S222), T217 (= T223), C218 (≠ A224), T220 (= T226)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
26% identity, 95% coverage: 4:285/297 of query aligns to 4:284/312 of 7pn0A

query
sites
7pn0A

I

L

L

I

F

F

S

S

L

G

P

Q

G

S

N

N

E

R

K

P

L

L

T

A

E

Q

L

A

M

I

A

A

T

E

K

A

M

L

D

G

A

L

E

P

V

L

G

G

K

K

A

S

T

T

L

T

R

L

N

R

F

F

P

A

D

N

G

D

E

N

S

L

Y

F

T

V

R

R

I

Y

L

E

S

E

D

S

V

L

K

R

D

E

K

G

C

D

V

V

V

F

L

I

V

V

C

Q

T

S

L

F

H

V

E

P

P

P

-

V

D

Q

E

D

K

H

L

L

L

M

P

E

L

L

Y

L

F

M

L

M

S

V

H

D

T

A

A

A

K

K

S

G

L

A

G

S

A

A

M

A

C

R

T

V

C

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

K

K

V

K

F

D

N

A

E

P

G

R

E

I

G

S

V

I

T

T

S

A

G

R

F

L

F

I

G

A

K

D

L

L

I

L

-

Q

S

T

G

A

F

G

A

A

D

D

S

R

I

V

T

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

I

V

S

H

S

G

L

F

G

-

E

-

V

-

Y

F

Q

K

I

I

P

P

N

V

K

D

V

H

I

L

H

S

A

A

A

E

D

P

A

V

I

I

S

A

D

N

W

Y

I

F

-

A

-

T

K

R

E

V

N

D

I

L

E

E

N

N

P

A

V

V

L

V

I

V

G

A

P

P

D

D

S

A

E

G

S

D

E

L

Q

K

W

R

V

A

S

S

E

A

V

L

A

A

K

R

N

R

A

L

G

G

A

L

P

P

F

L

I

A

V

F

L

I

Q

D

K

K

M

E

R

R

H

V

G

S

D

D

R

T

D

E

V

V

E

R

V

V

S

R

V

M

P

L

D

-

V

V

D

G

I

E

Y

V

K

E

D

G

A

K

T

T

P

A

I

L

L

I

V

V

D

D

I

E

I

I

S

S

T

T

A

A

R

G

T

S

M

L

I

V

E

E

T

A

V

V

Q

E

H

A

L

L

K

M

K

Q

A

A

G

G

M

A

K

K

P

E

P

V

I

Y

C

A

V

A

G

A

I

T

H

H

A

G

V

V

F

Y

S

V

G

G

N

P

S

A

Y

L

K

D

D

R

L

I

L

A

G

K

S

S

G

P

V

V

E

K

K

E

I

V

V

A

T

A

C

T

N

D

T

T

I

C

P

P

H

-

P

P

S

K

N

E

G

G

I

P

D

K

L

L

S

R

D

T

I

L

binding adenosine-5'-diphosphate: R95 (= R94), S96 (≠ Q95), H129 (= H127)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
26% identity, 95% coverage: 4:285/297 of query aligns to 3:283/307 of 5t3oA

query
sites
5t3oA

I

L

L

I

F

F

S

S

L

G

P

Q

G

S

N

N

E

R

K

P

L

L

T

A

E

Q

L

A

M

I

A

A

T

E

K

A

M

L

D

G

A

L

E

P

V

L

G

G

K

K

A

S

T

T

L

T

R

L

N

R

F

F

P

A

D

N

G

D

E

N

S

L

Y

F

T

V

R

R

I

Y

L

E

S

E

D
x
S

V

L

K
x
R

D

E

K

G

C

D

V

V

V

F

L

I

V

V

C

Q

T

S

L

F

H

V

E

P

P

P

-

V

D

Q

E

D

K

H

L

L

L

M

P

E

L

L

Y

L

F

M

L

M

S

V

H

D

T

A

A

A

K

K

S

G

L

A

G

S

A

A

M

A

C

R

T

V

C

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

S

Y

Y

M

A

R
|
R

Q
x
S

D

D

K

K

V

K

F
x
D

N

A

E

P

G

R

E

I

G

S

V

I

T

T

S

A

G

R

F

L

F

I

G

A

K

D

L

L

I

L

-

Q

S

T

G

A

F

G

A

A

D

D

S

R

I

V

T

L

T

T

V

M

D

T

P

L

H
|
H

L

S

H

P

R

Q

I

V

S

H

S

G

L

F

G

-

E

-

V

-

Y

F

Q

K

I

I

P

P

N

V

K

D

V

H

I

L

H

S

A

A

A

E

D

P

A

V

I

I

S

A

D

N

W

Y

I

F

-

A

-

T

K

R

E

V

N

D

I

L

E

E

N

N

P

A

V

V

L

V

I

V

G

A

P

P

D

D

S

A

E

G

S

D

E

L

Q

K

W

R

V

A

S

S

E

A

V

L

A

A

K

R

N

R

A

L

G

G

A

L

P

P

F

L

I

A

V

F

L

I

Q

D

K

K

M

E

R
|
R

H

V

G

S

D

D

R

T

D

E

V

V

E

R

V

V

S

R

V

M

P

L

D

-

V

V

D

G

I

E

Y

V

K

E

D

G

A

K

T

T

P

A

I

L

L

I

V

V

D

D

I

E

I

I

S

S

T

T

A

A

R

G

T

S

M

L

I

V

E

E

T

A

V

V

Q

E

H

A

L

L

K

M

K

Q

A

A

G

G

M

A

K

K

P

E

P

V

I

Y

C

A

V

A

G

A

I

T

H

H

A

G

V

V

F

Y

S

V

G

G

N

P

S

A

Y

L

K

D

D

R

L

I

L

A

G

K

S

S

G

P

V

V

E

K

K

E

I

V

V

A

T

A

C

T

N

D

T

T

I

C

P

P

H

-

P

P

S

K

N

E

G

G

I

P

D

K

L

L

S

R

D

T

I

L

binding adenosine-5'-diphosphate: R94 (= R94), S95 (≠ Q95), D99 (≠ F99), H128 (= H127), R193 (= R193)
binding sulfate ion: S45 (≠ D46), R47 (≠ K48)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 98% coverage: 5:295/297 of query aligns to 8:308/321 of O94413

query
sites
O94413

L

I

F

F

S

A

L

G

P

N

G

S

N

H

E

P

K

E

L

L

T

A

E

E

L

K

M

V

A

A

T

R

K

R

M

I

D

G

A

L

E

S

V

L

G

G

K

K

A

V

T

A

L

V

R

V

N

Q

F

Y

P

S

D

N

G

R

E

E

S

T

Y

S

T

V

R

T

I

I

L

G

S

E

D

S

V

V

K

R

D

D

K

E

C

D

V

V

V

F

L

I

V

L

C

Q

T

T

-

G

L

C

H

G

E

S

P

I

D

N

E

D

K

H

L

L

L

M

P

E

L

L

Y

L

F

I

L

M

S

I

H

N

T

A

A

C

K

R

S

S

L

A

G

S

A

A

M

R

C

R

T

I

C

T

L

A

V

I

A

I

P

P

Y

C

L

F

A

P

Y

Y

M

A

R

R

Q

Q

D

D

K

K

V

K

F

D

N

K

E

S

G

R

E

A

G

P

V

I

T

T

S

A

G

R

F

L

F

V

G

A

K

N

L

M

I

L

-

Q

S

T

G

A

F

G

A

C

D

N

S

H

I

I

T

I

T

T

V

M

D

D

P

L

H

H

L

A

H

S

R

Q

I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

Q

N

I

V

P

P

N

V

K

D

V

N

I

L

H

Y

A

A

A

E

D

P

A

S

I

V

S

L

D

R

W

Y

I

I

K

R

E

E

N

N

I

I

E

D

-

T

-

V

N

N

P

P

-

T

V

V

L

I

I

V

G
x
S

P

P

D

D

S

A

E

G

S

G

E

A

Q

K

W

R

V

A

S

T

E

A

V

L

A

A

K

D

N

R

A

L

G

D

A

L

P

D

F

F

I

A

V

L

L

I

Q

H

K

K

M

E

R

R

H

Q

G

K

D

A

R

N

D

E

V

V

E

S

V

R

S

M

V

V

P

L

D

V

V

G

D

D

I

V

Y

-

K

R

D

D

A

K

T

L

P

A

I

I

L

L

V

V

D

D

I

M

I

A

S

D

T

T

A

C

R

G

T

T

M

L

I

G

E

L

T

A

V

A

Q

K

H

T

L

L

K

K

K

D

A

N

G

G

M

A

K

K

P

A

P

V

I

Y

C

A

V

I

G

V

I

T

H

H

A

G

V

I

F

L

S

S

G

G

N

K

S

A

Y

I

K

K

D

V

L

I

L

N

G

E

S

S

G

A

V

L

E

E

K

K

I

V

V

I

T

V

C

T

N

N

T

T

I

I

P

P

H

H

P

D

S

D

N

K

-

R

-

S

-

L

-

C

-

S

-

K

-

I

-

E

G

T

I

I

D

D

L

I

S

S

D

G

I

V

M

L

A

A

K

E

E

C

V

I

K

R

L

I

M

H

H

H

S172 (≠ G166) modified: Phosphoserine

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
23% identity, 98% coverage: 5:296/297 of query aligns to 6:306/315 of P0A717

query
sites
P0A717

L

L

F

F

S

A

L

G

P

N

G

A

N

T

E

P

K

E

L

L

T

A

E

Q

L

R

M

I

A

A

T

N

K

R

M

L

D

Y

A

T

E

S

V

L

G

G

K

D

A

A

T

A

L

V

R

G

N

R

F

F

P

S

D

D

G

G

E

E

S

V

Y

S

T

V

R

Q

I

I

L

N

S

E

D

N

V

V

K

R

D

G

K

G

C

D

V

I

V

F

L

I

V

I

C

Q

T

S

L

T

H

C

E

A

P

P

-

T

D

N

E

D

K

N

L

L

L

M

P

E

L

L

Y

V

F

V

L

M

S

V

H

D

T

A

A

L

K

R

S

R

L

A

G

S

A

A

M

G

C

R

T

I

C

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

K

R

V

R

F

V

N

R

E

S

G

A

E

R

-

V

G

P

V

I

T

T

S

A

G

K

F

V

G

A

K

D

L

F

I

L

S

S

G

S

F

V

A

G

-

V

D

D

S

R

I

V

T

L

T

T

V

V

D
|
D

P

L

H

H

L

A

H

E

R

Q

I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

Q

D

I

V

P

P

-

V

N

D

K

N

V

V

I

F

H

G

A

S

A

P

D

I

A

L

I

L

S

E

D

D

W

M

I

L

K

Q

E

L

N

N

I

L

E

D

N

N

P

P

V

I

L

V

I

V

G

S

P

P

D

D

S

I

E

G

S

G

E

V

Q

V

W

R

V

A

S

R

E

A

V

I

A

A

K

K

N

L

A

L

G

N

A

D

P

T

F

D

I

M

V

A

L

I

Q

I

K

D

M

K

R

R

H

R

G

P

D

R

R

A

D

N

V

V

E

S

V

Q

S

V

V

M

P

H

D

I

V

I

D

G

I

D

Y

V

K

A

D

G

A

R

T

D

P

C

I

V

L

L

V

V

D
|
D

I

M

I

I

S
x
D

T

T

A

G

R

G

T

T

M

L

I

C

E

K

T

A

V

A

Q

E

H

A

L

L

K

K

K

E

A

R

G

G

M

A

K

K

P

R

P

V

I

F

C

A

V

Y

G

A

I

T

H

H

A

P

V

I

F

F

S

S

G

G

N

N

S

A

Y

A

K

N

D

N

L

L

L

R

G

N

S

S

G

V

V

I

E

D

K

E

I

V

V

V

T

V

C

C

N

D

T

T

I

I

P

P

H

L

P

S

S

D

-

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

N

R

G

T

I

L

D

T

L

L

S

S

D

G

I

M

M

L

A

A

K

E

E

A

V

I

K

R

L

I

M

S

H

N

Q

E

D129 (= D125) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D218) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D219) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ S222) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
23% identity, 98% coverage: 5:296/297 of query aligns to 5:305/308 of 4s2uA

query
sites
4s2uA

L

L

F

F

S

A

L

G

P

N

G

A

N

T

E

P

K

E

L

L

T

A

E

Q

L

R

M

I

A

A

T

N

K

R

M

L

D

Y

A

T

E

S

V

L

G

G

K

D

A

A

T

A

L

V

R

G

N

R

F

F

P

S

D

D

G

G

E

E

S

V

Y

S

T

V

R

Q

I

I

L

N

S

E

D

N

V

V

K

R

D

G

K

G

C

D

V

I

V

F

L

I

V

I

C

Q

T

S

L

T

H

C

E

A

P

P

-

T

D

N

E

D

K

N

L

L

L

M

P

E

L

L

Y

V

F

V

L

M

S

V

H

D

T

A

A

L

K

R

S

R

L

A

G

S

A

A

M

G

C

R

T

I

C

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

K

R

V

R

F

V

N

R

E

S

G

A

E

R

-

V

G

P

V

I

T

T

S

A

G

K

F

V

G

A

K

D

L

F

I

L

S

S

G

S

F

V

A

G

-

V

D

D

S

R

I

V

T

L

T

T

V

V

D

D

P

L

H

H

L

A

H

E

R

Q

I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

Q

D

I

V

P

P

-

V

N

D

K

N

V

V

I

F

H

G

A

S

A

P

D

I

A

L

I

L

S

E

D

D

W

M

I

L

K

Q

E

L

N

N

I

L

E

D

N

N

P

P

V

I

L

V

I

V

G

S

P

P

D

D

S

I

E

G

S

G

E

V

Q

V

W

R

V

A

S

R

E

A

V

I

A

A

K

K

N

L

A

L

G

N

A

D

P

T

F

D

I

M

V

A

L

I

Q

I

K

D

M

K

R

R

H

R

G

P

D

R

R

A

D

N

V

V

E

S

V

Q

S

V

V

M

P

H

D

I

V

I

D

G

I

D

Y

V

K

A

D

G

A

R

T

D

P

C

I

V

L

L

V

V

D

D

I

M

I

I

S
x
D

T

T

A

G

R

G

T
|
T

M

L

I

C

E

K

T

A

V

A

Q

E

H

A

L

L

K

K

K

E

A

R

G

G

M

A

K

K

P

R

P

V

I

F

C

A

V

Y

G

A

I

T

H

H

A

P

V

I

F

F

S

S

G

G

N

N

S

A

Y

A

K

N

D

N

L

L

L

R

G

N

S

S

G

V

V

I

E

D

K

E

I

V

V

V

T

V

C

C

N

D

T

T

I

I

P

P

H

L

P

S

S

D

-

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

N

R

G

T

I

L

D

T

L

L

S

S

D

G

I

M

M

L

A

A

K

E

E

A

V

I

K

R

L

I

M

S

H

N

Q

E

binding magnesium ion: D223 (≠ S222), T227 (= T226)

6asvC E. Coli prpp synthetase (see paper)
23% identity, 98% coverage: 5:296/297 of query aligns to 4:304/311 of 6asvC

query
sites
6asvC

L

L

F

F

S

A

L

G

P

N

G

A

N

T

E

P

K

E

L

L

T

A

E

Q

L

R

M

I

A

A

T

N

K

R

M

L

D

Y

A

T

E

S

V

L

G

G

K

D

A

A

T

A

L

V

R

G

N

R

F

F

P

S

D

D

G

G

E

E

S

V

Y

S

T

V

R

Q

I

I

L

N

S

E

D

N

V

V

K

R

D

G

K

G

C

D

V

I

V

F

L

I

V

I

C

Q

T

S

L

T

H

C

E

A

P

P

-

T

D

N

E

D

K

N

L

L

L

M

P

E

L

L

Y

V

F

V

L

M

S

V

H

D

T

A

A

L

K

R

S

R

L

A

G

S

A

A

M

G

C

R

T

I

C

T

L

A

V

V

A

I

P

P

Y

Y

L

F

A

G

Y

Y

M

A

R

R

Q

Q

D

D

K

R

V

R

F

V

N

R

E

S

G

A

E

R

-

V

G

P

V

I

T

T

S

A

G

K

F

V

G

A

K

D

L

F

I

L

S

S

G

S

F

V

A

G

-

V

D

D

S

R

I

V

T

L

T

T

V

V

D

D

P

L

H

H

L

A

H

E

R

Q

I

I

S

Q

S

G

L

F

G

-

E

-

V

-

Y

F

Q

D

I

V

P

P

-

V

N

D

K

N

V

V

I

F

H

G

A

S

A

P

D

I

A

L

I

L

S

E

D

D

W

M

I

L

K

Q

E

L

N

N

I

L

E

D

N

N

P

P

V

I

L

V

I

V

G

S

P

P

D
|
D

S

I

E

G

S

G

E

V

Q

V

W

R

V

A

S

R

E

A

V

I

A

A

K

K

N

L

A

L

G

N

A

D

P

T

F

D

I

M

V

A

L

I

Q

I

K

D

M

K

R

R

H

R

G

P

D

R

R

A

D

N

V

V

E

S

V

Q

S

V

V

M

P

H

D

I

V

I

D

G

I

D

Y

V

K

A

D

G

A

R

T

D

P

C

I

V

L

L

V

V

D
|
D

I

M

I

I

S
x
D

T
|
T

A
x
G

R

G

T
|
T

M
x
L

I

C

E

K

T

A

V

A

Q

E

H

A

L

L

K

K

K

E

A

R

G

G

M

A

K

K

P

R

P

V

I

F

C

A

V

Y

G

A

I

T

H

H

A

P

V

I

F

F

S

S

G

G

N

N

S

A

Y

A

K

N

D

N

L

L

L

R

G

N

S

S

G

V

V

I

E

D

K

E

I

V

V

V

T

V

C

C

N

D

T

T

I

I

P

P

H

L

P

S

S

D

-

E

-

I

-

K

-

S

-

L

-

P

-

N

-

V

N

R

G

T

I

L

D

T

L

L

S

S

D

G

I

M

M

L

A

A

K

E

E

A

V

I

K

R

L

I

M

S

H

N

Q

E

binding phosphate ion: D168 (= D168), D218 (= D218), D219 (= D219), D222 (≠ S222), T223 (= T223), G224 (≠ A224), T226 (= T226), L227 (≠ M227)

Query Sequence

>Echvi_3167 Echvi_3167 ribose-phosphate pyrophosphokinase
MKTILFSLPGNEKLTELMATKMDAEVGKATLRNFPDGESYTRILSDVKDKCVVLVCTLHE
PDEKLLPLYFLSHTAKSLGAMCTCLVAPYLAYMRQDKVFNEGEGVTSGFFGKLISGFADS
ITTVDPHLHRISSLGEVYQIPNKVIHAADAISDWIKENIENPVLIGPDSESEQWVSEVAK
NAGAPFIVLQKMRHGDRDVEVSVPDVDIYKDATPILVDDIISTARTMIETVQHLKKAGMK
PPICVGIHAVFSGNSYKDLLGSGVEKIVTCNTIPHPSNGIDLSDIMAKEVKKLMHQI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory