SitesBLAST
Comparing Echvi_3438 FitnessBrowser__Cola:Echvi_3438 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
5vt3B High resolution structure of thioredoxin-disulfide reductase from vibrio vulnificus cmcp6 in complex with NADP and fad
56% identity, 96% coverage: 9:308/314 of query aligns to 10:315/319 of 5vt3B
- active site: C138 (= C138), C141 (= C141), D142 (= D142)
- binding flavin-adenine dinucleotide: G15 (= G14), S16 (= S15), G17 (= G16), P18 (= P17), A19 (= A18), T38 (= T37), G39 (= G38), Q41 (= Q40), G44 (= G43), Q45 (= Q44), L46 (= L45), T49 (= T48), N54 (= N53), H86 (≠ L85), I87 (≠ V86), S114 (= S114), T115 (= T115), G116 (= G116), E162 (= E162), H247 (= H243), G287 (= G280), D288 (= D281), R295 (= R288), Q296 (= Q289), A297 (= A290), S300 (≠ A293)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L122 (= L122), G156 (≠ A156), G157 (= G157), N158 (≠ D158), T159 (= T159), H178 (≠ V178), R179 (= R179), R180 (= R180), R184 (= R184), I245 (= I241), G246 (= G242), R295 (= R288), Q296 (= Q289)
5w4cA Crystal structure of thioredoxin reductase from cryptococcus neoformans in complex with fad (fo conformation)
52% identity, 97% coverage: 9:312/314 of query aligns to 19:334/356 of 5w4cA
- binding calcium ion: E99 (≠ G84), E116 (≠ D101), E118 (≠ K103)
- binding flavin-adenine dinucleotide: I23 (≠ V13), G24 (= G14), S25 (= S15), P27 (= P17), G28 (≠ A18), Y46 (= Y36), G48 (vs. gap), A51 (vs. gap), F54 (≠ G39), G58 (= G43), Q59 (= Q44), L60 (= L45), T63 (= T48), N68 (= N53), V101 (= V86), T134 (= T115), G135 (= G116), G302 (= G280), D303 (= D281), R310 (= R288), Q311 (= Q289), A312 (= A290), S315 (≠ A293)
Sites not aligning to the query:
8ccmA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with compound 2-06
50% identity, 96% coverage: 10:311/314 of query aligns to 5:305/305 of 8ccmA
- binding flavin-adenine dinucleotide: I8 (≠ V13), G9 (= G14), S10 (= S15), G11 (= G16), P12 (= P17), A13 (= A18), E32 (≠ T37), G33 (= G38), Q35 (= Q40), G38 (= G43), A39 (≠ Q44), L40 (= L45), T43 (= T48), N48 (= N53), D80 (≠ L85), V81 (= V86), M109 (≠ T115), G110 (= G116), T131 (≠ A137), C135 (= C141), G274 (= G280), D275 (= D281), R282 (= R288), Q283 (= Q289), A284 (= A290), A287 (= A293)
- binding ~{N}6-(4-aminophenyl)-1,2-benzothiazole-3,6-diamine: R114 (≠ K120), H115 (≠ W121), L116 (= L122), R173 (= R179), E200 (= E206), I201 (≠ T207), I235 (= I241)
8cclA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry a09
50% identity, 96% coverage: 10:311/314 of query aligns to 5:305/305 of 8cclA
- binding flavin-adenine dinucleotide: I8 (≠ V13), G9 (= G14), S10 (= S15), G11 (= G16), P12 (= P17), A13 (= A18), E32 (≠ T37), G33 (= G38), Q35 (= Q40), G38 (= G43), A39 (≠ Q44), L40 (= L45), T43 (= T48), N48 (= N53), D80 (≠ L85), V81 (= V86), M109 (≠ T115), G110 (= G116), T131 (≠ A137), C135 (= C141), G274 (= G280), D275 (= D281), R282 (= R288), Q283 (= Q289), A284 (= A290), A287 (= A293)
- binding [1,2]thiazolo[5,4-b]pyridin-3-amine: L116 (= L122), R173 (= R179), E200 (= E206), I201 (≠ T207)
8cckA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry h07
50% identity, 96% coverage: 10:311/314 of query aligns to 5:305/305 of 8cckA
- binding flavin-adenine dinucleotide: G9 (= G14), S10 (= S15), G11 (= G16), P12 (= P17), A13 (= A18), E32 (≠ T37), G33 (= G38), Q35 (= Q40), G38 (= G43), A39 (≠ Q44), L40 (= L45), T43 (= T48), N48 (= N53), D80 (≠ L85), V81 (= V86), M109 (≠ T115), G110 (= G116), T131 (≠ A137), C135 (= C141), G274 (= G280), D275 (= D281), R282 (= R288), Q283 (= Q289), A284 (= A290), A287 (= A293)
- binding ~{N}-(4-hydroxyphenyl)-2-pyrazol-1-yl-ethanamide: R114 (≠ K120), H115 (≠ W121), L116 (= L122), V148 (= V154), R173 (= R179), E200 (= E206), I201 (≠ T207)
8ccjA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with NADPH
50% identity, 96% coverage: 10:311/314 of query aligns to 5:305/305 of 8ccjA
- binding flavin-adenine dinucleotide: I8 (≠ V13), G9 (= G14), S10 (= S15), G11 (= G16), P12 (= P17), A13 (= A18), E32 (≠ T37), G33 (= G38), Q35 (= Q40), G38 (= G43), A39 (≠ Q44), L40 (= L45), T43 (= T48), N48 (= N53), D80 (≠ L85), V81 (= V86), M109 (≠ T115), G110 (= G116), T131 (≠ A137), C135 (= C141), G274 (= G280), D275 (= D281), R282 (= R288), Q283 (= Q289), A284 (= A290), A287 (= A293)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G150 (≠ A156), G151 (= G157), D152 (= D158), S153 (≠ T159), E156 (= E162), H172 (≠ V178), R173 (= R179), R174 (= R180), R178 (= R184), I235 (= I241)
5uthA Crystal structure of thioredoxin reductase from mycobacterium smegmatis in complex with fad
50% identity, 96% coverage: 10:311/314 of query aligns to 6:306/306 of 5uthA
- active site: C133 (= C138), C136 (= C141), D137 (= D142)
- binding flavin-adenine dinucleotide: I9 (≠ V13), G10 (= G14), S11 (= S15), G12 (= G16), P13 (= P17), A14 (= A18), F32 (≠ Y36), E33 (≠ T37), G34 (= G38), Q36 (= Q40), G39 (= G43), A40 (≠ Q44), L41 (= L45), N49 (= N53), D81 (≠ L85), V82 (= V86), M110 (≠ T115), G111 (= G116), C136 (= C141), G275 (= G280), D276 (= D281), R283 (= R288), Q284 (= Q289), A285 (= A290), A288 (= A293)
4jnqA Crystal structure of a thioredoxin reductase from brucella melitensis
52% identity, 96% coverage: 10:312/314 of query aligns to 9:315/315 of 4jnqA
- active site: C137 (= C138), C140 (= C141), D141 (= D142)
- binding dihydroflavine-adenine dinucleotide: I12 (≠ V13), G13 (= G14), S14 (= S15), G15 (= G16), P16 (= P17), A17 (= A18), A36 (≠ T37), G37 (= G38), Q39 (= Q40), G42 (= G43), Q43 (= Q44), L44 (= L45), N52 (= N53), I85 (≠ V86), A113 (≠ S114), T114 (= T115), C140 (= C141), G283 (= G280), D284 (= D281), R291 (= R288), Q292 (= Q289), A293 (= A290)
P0A9P4 Thioredoxin reductase; TRXR; EC 1.8.1.9 from Escherichia coli (strain K12) (see 2 papers)
52% identity, 96% coverage: 9:308/314 of query aligns to 8:314/321 of P0A9P4
- 36:43 (vs. 37:44, 63% identical) binding
- C136 (= C138) modified: Disulfide link with 139, Redox-active
- C139 (= C141) modified: Disulfide link with 136, Redox-active
- 287:296 (vs. 281:290, 70% identical) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1f6mA Crystal structure of a complex between thioredoxin reductase, thioredoxin, and the NADP+ analog, aadp+ (see paper)
52% identity, 96% coverage: 9:308/314 of query aligns to 7:313/320 of 1f6mA
- active site: S135 (≠ C138), C138 (= C141), D139 (= D142)
- binding 3-aminopyridine-adenine dinucleotide phosphate: L119 (= L122), G153 (≠ A156), G154 (= G157), N155 (≠ D158), T156 (= T159), E159 (= E162), H175 (≠ V178), R176 (= R179), R177 (= R180), R181 (= R184), I243 (= I241), G244 (= G242), H245 (= H243), R293 (= R288), Q294 (= Q289)
- binding flavin-adenine dinucleotide: G12 (= G14), S13 (= S15), G14 (= G16), P15 (= P17), A16 (= A18), T35 (= T37), G36 (= G38), E38 (≠ Q40), G41 (= G43), Q42 (= Q44), L43 (= L45), T46 (= T48), V49 (= V51), N51 (= N53), H83 (≠ L85), I84 (≠ V86), A111 (≠ S114), T112 (= T115), G113 (= G116), H245 (= H243), G285 (= G280), D286 (= D281), R293 (= R288), Q294 (= Q289), A295 (= A290), S298 (≠ A293)
1tdfA Crystal structure of escherichia coli thioredoxin reductase refined at 2 angstrom resolution: implications for a large conformational change during catalysis (see paper)
52% identity, 96% coverage: 9:308/314 of query aligns to 7:313/316 of 1tdfA
- active site: C135 (= C138), S138 (≠ C141), D139 (= D142)
- binding flavin-adenine dinucleotide: G12 (= G14), S13 (= S15), G14 (= G16), P15 (= P17), A16 (= A18), T35 (= T37), G36 (= G38), E38 (≠ Q40), G41 (= G43), Q42 (= Q44), L43 (= L45), T46 (= T48), V49 (= V51), N51 (= N53), H83 (≠ L85), I84 (≠ V86), A111 (≠ S114), T112 (= T115), S138 (≠ C141), G285 (= G280), D286 (= D281), R293 (= R288), Q294 (= Q289), A295 (= A290), S298 (≠ A293)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L119 (= L122), I151 (≠ V154), T156 (= T159), E159 (= E162), H175 (≠ V178), R176 (= R179), R181 (= R184), E183 (≠ S186), I243 (= I241), G244 (= G242), H290 (= H285), R293 (= R288)
6bpyA Aspergillus fumigatus thioredoxin reductase (see paper)
49% identity, 97% coverage: 9:312/314 of query aligns to 4:323/324 of 6bpyA
- binding flavin-adenine dinucleotide: I8 (≠ V13), G9 (= G14), S10 (= S15), G11 (= G16), P12 (= P17), A13 (= A18), Y31 (= Y36), E32 (≠ T37), G33 (= G38), A36 (vs. gap), T38 (≠ G39), A39 (≠ Q40), G42 (= G43), Q43 (= Q44), L44 (= L45), T47 (= T48), I50 (≠ V51), N52 (= N53), T84 (≠ L85), I85 (≠ V86), T120 (= T115), G121 (= G116), C146 (= C141), G291 (= G280), D292 (= D281), R299 (= R288), Q300 (= Q289), A301 (= A290), S304 (≠ A293)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G163 (≠ A156), G164 (= G157), S166 (≠ T159), R186 (= R179), R187 (= R180), R191 (= R184), V252 (≠ I241)
P29509 Thioredoxin reductase 1; TR; TrxR; Thioredoxin peroxidase 1; TPx; Thioredoxin-dependent peroxide reductase 1; EC 1.8.1.9 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
49% identity, 97% coverage: 9:312/314 of query aligns to 5:319/319 of P29509
- SGPA 11:14 (= SGPA 15:18) binding
- IA 40:41 (vs. gap) binding
- Q45 (= Q44) binding
- N54 (= N53) binding
- V87 (= V86) binding
- C142 (= C138) modified: Disulfide link with 145, Redox-active
- C145 (= C141) binding ; modified: Disulfide link with 142, Redox-active
- D288 (= D281) binding
- RQA 295:297 (= RQA 288:290) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
3d8xA Crystal structure of saccharomyces cerevisiae ndpph dependent thioredoxin reductase 1 (see paper)
49% identity, 97% coverage: 9:312/314 of query aligns to 4:318/318 of 3d8xA
- active site: C141 (= C138), C144 (= C141), D145 (= D142)
- binding flavin-adenine dinucleotide: I8 (≠ V13), S10 (= S15), G11 (= G16), P12 (= P17), A13 (= A18), Y31 (= Y36), G33 (= G38), A36 (≠ P41), I39 (vs. gap), G43 (= G43), Q44 (= Q44), I51 (≠ V51), N53 (= N53), T85 (≠ L85), V86 (= V86), T118 (= T115), G119 (= G116), C144 (= C141), G286 (= G280), D287 (= D281), R294 (= R288), Q295 (= Q289), A296 (= A290), S299 (≠ A293)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: M125 (≠ L122), G160 (= G155), S164 (≠ T159), R184 (= R179), K185 (≠ R180), R189 (= R184), I247 (= I241)
3itjB Crystal structure of saccharomyces cerevisiae thioredoxin reductase 1 (trr1) (see paper)
49% identity, 97% coverage: 9:312/314 of query aligns to 5:319/319 of 3itjB
- active site: C142 (= C138), C145 (= C141), D146 (= D142)
- binding flavin-adenine dinucleotide: I9 (≠ V13), G10 (= G14), S11 (= S15), G12 (= G16), P13 (= P17), A14 (= A18), Y32 (= Y36), E33 (≠ T37), G34 (= G38), A37 (≠ P41), I40 (vs. gap), A41 (vs. gap), G44 (= G43), Q45 (= Q44), T49 (= T48), I52 (≠ V51), N54 (= N53), T86 (≠ L85), V87 (= V86), T119 (= T115), G120 (= G116), W135 (≠ N131), C145 (= C141), G287 (= G280), D288 (= D281), R295 (= R288), Q296 (= Q289), A297 (= A290), S300 (≠ A293)
5u63B Crystal structure of putative thioredoxin reductase from haemophilus influenzae
51% identity, 96% coverage: 9:308/314 of query aligns to 11:316/319 of 5u63B
- active site: C139 (= C138), C142 (= C141), D143 (= D142)
- binding flavin-adenine dinucleotide: G16 (= G14), S17 (= S15), G18 (= G16), P19 (= P17), A20 (= A18), T39 (= T37), G40 (= G38), Q42 (= Q40), G45 (= G43), Q46 (= Q44), L47 (= L45), T50 (= T48), N55 (= N53), H87 (≠ L85), I88 (≠ V86), A115 (≠ S114), T116 (= T115), G117 (= G116), H248 (= H243), G288 (= G280), D289 (= D281), R296 (= R288), Q297 (= Q289), A298 (= A290), S301 (≠ A293)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R121 (≠ K120), G157 (≠ A156), H179 (≠ V178), R180 (= R179), R181 (= R180), I246 (= I241), G247 (= G242), H248 (= H243), R296 (= R288)
Q70G58 Thioredoxin reductase NTRC; NADPH-dependent thioredoxin reductase C; OsNTRC; EC 1.8.1.9 from Oryza sativa subsp. japonica (Rice) (see 2 papers)
48% identity, 97% coverage: 10:313/314 of query aligns to 72:382/515 of Q70G58
- C203 (= C138) mutation to S: Loss of thioredoxin reductase activity.
- C206 (= C141) mutation to S: Loss of thioredoxin reductase activity.
- A227 (= A160) mutation to G: Reduces activity 30-fold; when associated with E-245 and F-246.
- V245 (= V178) mutation to E: Reduces activity 30-fold; when associated with G-227 and F-246.
- R246 (= R179) mutation to F: Reduces activity 30-fold; when associated with G-227 and E-245.
Sites not aligning to the query:
- 440 C→S: Loss of thioredoxin activity.
- 443 C→S: Loss of thioredoxin activity.
Q39243 Thioredoxin reductase 1, mitochondrial; NADPH-dependent thioredoxin reductase 1; NTR1; NADPH-dependent thioredoxin reductase B; AtNTRB; EC 1.8.1.9 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
50% identity, 98% coverage: 4:310/314 of query aligns to 47:366/375 of Q39243
- SGPA 58:61 (= SGPA 15:18) binding
- FE 79:80 (≠ YT 36:37) binding
- IAPGGQ 87:92 (≠ --PGGQ 41:44) binding
- N101 (= N53) binding
- V134 (= V86) binding
- C189 (= C138) modified: Disulfide link with 192, Redox-active
- C192 (= C141) binding ; modified: Disulfide link with 189, Redox-active
- D337 (= D281) binding
- RQA 344:346 (= RQA 288:290) binding
Sites not aligning to the query:
- 1:37 modified: transit peptide, Mitochondrion
P9WHH1 Thioredoxin reductase; TR; TRXR; EC 1.8.1.9 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
49% identity, 96% coverage: 10:311/314 of query aligns to 17:318/335 of P9WHH1
- SGPA 22:25 (= SGPA 15:18) binding
- Y32 (= Y25) modified: Phosphotyrosine; by PtkA; mutation to A: Significantly reduces phosphorylation.
- 44:51 (vs. 37:44, 38% identical) binding
- N60 (= N53) binding
- V93 (= V86) binding
- C145 (= C138) modified: Disulfide link with 148, Redox-active
- C148 (= C141) modified: Disulfide link with 145, Redox-active
- S166 (≠ T159) binding
- H185 (≠ V178) binding
- R191 (= R184) binding
- I248 (= I241) binding
- Y268 (= Y261) binding
- D288 (= D281) binding
- R295 (= R288) binding
- RQAV 295:298 (= RQAV 288:291) binding
2a87A Crystal structure of m. Tuberculosis thioredoxin reductase (see paper)
49% identity, 96% coverage: 10:311/314 of query aligns to 8:309/313 of 2a87A
- active site: F39 (≠ P41), L43 (= L45), D48 (= D50), C136 (= C138), C139 (= C141), D140 (= D142)
- binding flavin-adenine dinucleotide: G12 (= G14), S13 (= S15), G14 (= G16), P15 (= P17), A16 (= A18), F34 (≠ Y36), E35 (≠ T37), G36 (= G38), G40 (= G42), G41 (= G43), A42 (≠ Q44), L43 (= L45), T46 (= T48), V49 (= V51), N51 (= N53), D83 (≠ L85), V84 (= V86), M113 (≠ T115), C139 (= C141), G278 (= G280), D279 (= D281), R286 (= R288), Q287 (= Q289), A288 (= A290), V289 (= V291)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L120 (= L122), G155 (= G157), D156 (= D158), S157 (≠ T159), H176 (≠ V178), R177 (= R179), R178 (= R180), R182 (= R184), I239 (= I241), Y259 (= Y261), R283 (≠ H285), R286 (= R288)
Query Sequence
>Echvi_3438 FitnessBrowser__Cola:Echvi_3438
MNKEAEKIKVLIVGSGPAGYTAAIYASRAGLSPVLYTGGQPGGQLTITNDVENYPGYPDG
VMGPQMMEDFKKQAERFGTDVRYGLVTAVDFSTGPHKVIVDDKDEILADTVIISTGASAK
WLGLESETKLNGKGVSACAVCDGFFFRNQKVAIVGAGDTACEEASYLANICEKVYMLVRR
DEMRASQIMQKRVTSNPKIEILWNTETEEILGEEEVTGVRVKNNQTGEEQELEVTGFFVA
IGHKPNTDIFKDFLDMNEAGYINTQPGSTKTNIEGVFACGDAQDHVYRQAVTAAGTGCMS
ALDAERFLAEKELA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory