SitesBLAST

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
38% identity, 95% coverage: 1:180/189 of query aligns to 4:183/183 of 3nz2C

query
sites
3nz2C

M

L

E

E

K

K

M

M

M

L

A

K

G

G

E

E

P

H

Y

F

D

D

A

G

H

A

C

S

E

A

D

E

M

I

I

E

R

A

I

L

R

R

T

S

R

Q

V

A

K

G

R

R

I

L

L

K

H

L

K

E

L

I

N

N

V

Q

T

S

E

L

Y

D

Y

E

T

A

D

E

K

R

F

Y

Q

-

A

A

T

L

I

Q

N

R

E

E

L

L

C

F

P

G

N

H

S

L

A

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K

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E

Q

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P

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F

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C

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Y

F

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N

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I

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A

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I

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N

N

F

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D

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G

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A

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L

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G

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I

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Y
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Y

T

T

A

A

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H
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H

P

S

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L

N

D

V

Y

E

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Q

E

A

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W

E

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-

I

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G

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N

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N

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Q

G

G

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T

I

I

G

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D

A

R

R

A

S

V

V

I

V

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x
A

A
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A

G
x
N

A

S

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V

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x
N

K

Q

D

D

I

V

P

P

A

P

D

D

S

T

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L

A

V

V
x
G

G

G

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x
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P

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I
x
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R

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L

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K

E

D

binding acetyl coenzyme *a: Y107 (= Y105), H111 (= H109), G137 (= G134), A155 (≠ G152), A156 (= A153), N161 (≠ T158), G171 (≠ V168), T173 (≠ N170), P174 (= P171), L178 (≠ I175), R179 (≠ K176)
binding magnesium ion: N157 (≠ G154), T173 (≠ N170)

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
38% identity, 94% coverage: 1:178/189 of query aligns to 7:184/185 of 3nz2J

query
sites
3nz2J

M

L

E

E

K

K

M

M

M

L

A

K

G

G

E

E

P

H

Y

F

D

D

A

G

H

A

C

S

E

A

D

E

M

I

I

E

R

A

I

L

R

R

T

S

R

Q

V

A

K

G

R

R

I

L

L

K

H

L

K

E

L

I

N

N

V

Q

T

S

E

L

Y

D

Y

E

T

A

D

E

K

R

F

Y

Q

-

A

A

T

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R

E

E

L

L

C

F

P

G

N

H

S

L

A

G

K

H

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K

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E

Q

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P

F

F

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C

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N

T

I

I

H

R

A

I

G

G

E

D

G

H

V

T

F

F

I

I

N

N

F

M

D

N

A

V

V

V

I

M

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L

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D

G

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P

V

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T

I

I

G

G

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K

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T

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L

L

I

A
x
G

P

P

G

S

V

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Y
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Y

T

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A

A

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H
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H

P

S

L

L

N

D

V

Y

E

R

R

R

R

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A

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W

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I

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W
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G

G

G

H

N

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I

C

N

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G

G

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x
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A
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N

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x
N

K

Q

D

D

I

V

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x
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x
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x
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L

L

binding acetyl coenzyme *a: G104 (≠ A99), Y110 (= Y105), H114 (= H109), W138 (= W132), G140 (= G134), A158 (≠ G152), A159 (= A153), N164 (≠ T158), G174 (≠ V168), T176 (≠ N170), P177 (= P171), R182 (≠ K176)

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 99% coverage: 2:188/189 of query aligns to 7:195/203 of P07464

query
sites
P07464

E

E

K

R

M

I

M

R

A

A

G

G

E

K

P

L

Y

F

D

T

A
x
D

H

M

C

C

E

E

D

G

M

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I

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R

R

V

G

K

K

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L

L

M

H

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K

E

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N

N

V

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H

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P

Y

S

Y

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T

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E

K

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A

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I

N

K

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E

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A

N

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G

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N

L

A

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L

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E

P

P

F

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C

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D
x
S

Y

Y

G

G

Q

S

N

N

I

I

H

H

A

I

G

G

E

R

G

N

V

F

F

Y

I

A

N
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N

F

F

D

N

A

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I

I

L

V

D

D

G
x
D

A

Y

K

T

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V

T

T

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I

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G

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N

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L

L

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A

A

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H
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H

P

P

L

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N

H

V

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x
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A

G

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K
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K

D

D

I

I

P

P

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D

N

S

V

L

V

A

A

V

A

G

G

N

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P

A

C

K
x
R

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x
R

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N

N

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Y

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K

D17 (≠ A12) binding
S71 (≠ D65) binding
N85 (= N79) binding in other chain; binding in other chain
D93 (≠ G87) binding
H115 (= H109) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (≠ G135) binding in other chain
A160 (= A153) binding in other chain
TK 165:166 (= TK 158:159) binding
R180 (≠ K173) binding
R183 (≠ K176) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial

1krvA Galactoside acetyltransferase in complex with coa and pnp-beta-gal (see paper)
39% identity, 99% coverage: 2:188/189 of query aligns to 6:194/201 of 1krvA

query
sites
1krvA

E

E

K

R

M

I

M

R

A

A

G

G

E

K

P

L

Y

F

D

T

A
x
D

H

M

C

C

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K

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L

M

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E

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P

F

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S

Y

Y

G

G

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S

N

N

I

I

H

H

A

I

G

G

E

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G

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V

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F
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Y

I

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F

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x
S

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P

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I

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A

G

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I

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L

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N

N

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K

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D

H

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K

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Y

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K

binding 4-nitrophenyl beta-D-galactopyranoside: D16 (≠ A12), R25 (= R21), S70 (≠ D65), Y82 (≠ F77), N84 (= N79), V90 (≠ L85), D92 (≠ G87), M126 (vs. gap)
binding coenzyme a: A104 (= A99), S110 (≠ Y105), T112 (≠ A107), W138 (= W132), G140 (= G134), S141 (≠ G135), N146 (≠ C140), A159 (= A153), P177 (= P171), R179 (≠ K173), R182 (≠ K176)

1kruA Galactoside acetyltransferase in complex with iptg and coenzyme a (see paper)
39% identity, 99% coverage: 2:188/189 of query aligns to 6:194/201 of 1kruA

query
sites
1kruA

E

E

K

R

M

I

M

R

A

A

G

G

E

K

P

L

Y

F

D

T

A
x
D

H

M

C

C

E

E

D

G

M

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x
P

R

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R
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R

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R

R

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G

K

K

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L

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M

H

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K

E

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N

N

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E

H

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P

Y

S

Y

E

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E

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K

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Q

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I

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K

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E

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N

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x
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V

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E

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F

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S

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Y

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G

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N

N

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H

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A

I

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E

R

G

N

V

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F
x
Y

I

A

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N

F

F

D

N

A

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I

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x
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x
D

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Y

K

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T

I

I

G

G

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T

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L
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L

L

I

A

A

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P

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N

V

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Y

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H
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H

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M

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W

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G

G
x
S

H

H

V

V

T

V

I

I

C

N

P

P

G

G

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T

T

I

I

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G

D

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G

A
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A

G

G

A

S

V

I

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T

T

K

K

D

D

I

I

P

P

A

P

D

N

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L

V

A

A

V

A

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P

A

C

K

R

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V

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K
x
R

T

E

L

I

N

N

E

D

K

R

D

D

H

K

K

H

T

Y

Y

F

K

K

binding coenzyme a: H114 (= H109), W138 (= W132), G140 (= G134), S141 (≠ G135), G158 (= G152), A159 (= A153), P177 (= P171), R182 (≠ K176)
binding 1-methylethyl 1-thio-beta-D-galactopyranoside: D16 (≠ A12), P22 (≠ I18), R25 (= R21), W62 (≠ H57), S70 (≠ D65), Y82 (≠ F77), Y82 (≠ F77), N84 (= N79), V90 (≠ L85), D92 (≠ G87), L102 (= L97), H114 (= H109), M126 (vs. gap)

1krrA Galactoside acetyltransferase in complex with acetyl-coenzyme a (see paper)
39% identity, 99% coverage: 2:188/189 of query aligns to 6:194/200 of 1krrA

query
sites
1krrA

E

E

K

R

M

I

M

R

A

A

G

G

E

K

P

L

Y

F

D

T

A

D

H

M

C

C

E

E

D

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E

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K

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T

L

R

R

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G

K

K

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I

L

L

M

H

Y

K

E

L

F

N

N

V

H

T

S

E

H

-

P

Y

S

Y

E

T

V

D

E

K

K

F

R

Q

E

A

S

T

L

I

I

N

K

E

E

L

M

C

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A

N

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V

A

G

K

E

D

N

L

A

H

W

L

V

E

E

P

P

F

V

H

Y

C

F

D

S

Y

Y

G

G

Q

S

N

N

I

I

H

H

A

I

G

G

E

R

G

N

V

F

F

Y

I

A

N
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N

F

F

D

N

A

L

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T

I

I

L

V

D

D

G

D

A

Y

K

T

V

V

T

T

I

I

G

G

R

D

K

N

T

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L

L

L
x
I

A
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A

P
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P

G

N

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V

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T

I

L

Y

S

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x
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H
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H

P

P

L

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N

H

V

H

E

E

E

L

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R

R

K

E

N

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E

E

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M

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T

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W

I

I

G
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G

G
x
S

H

H

V

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T

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I

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C
x
N

P

P

G

G

V

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T

T

I

I

G

G

D

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R

N

A

S

V

V

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I

G
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G

A
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A

G

G

A

S

V

I

V

V

T

T

K

K

D

D

I

I

P

P

A

P

D

N

S

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L

V

A

A

V
x
A

G

G

N

V

P
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P

A

C

K

R

V

V

I

I

K
x
R

T

E

L

I

N

N

E

D

K

R

D

D

H

K

K

H

T

Y

Y

F

K

K

binding acetyl coenzyme *a: N84 (= N79), I103 (≠ L98), A104 (= A99), P105 (= P100), T112 (≠ A107), H114 (= H109), G140 (= G134), S141 (≠ G135), N146 (≠ C140), G158 (= G152), A159 (= A153), A174 (≠ V168), P177 (= P171), R182 (≠ K176)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
36% identity, 95% coverage: 1:180/189 of query aligns to 1:176/176 of 3ectA

query
sites
3ectA

M

L

E

E

K

K

M

M

M

L

A

K

G

G

E

H

P

A

Y

-

D

-

A

-

H

-

C

S

E

A

D

E

M

I

I

E

R

A

I

L

R

R

T

S

R

Q

V

A

K

G

R

R

I

L

L

K

H

L

K

E

L

I

N

N

V

Q

T

S

E

L

Y

D

Y

E

T

A

D

E

K

R

F

Y

Q

-

A

A

T

L

I

Q

N

R

E

E

L

L

C

F

P

G

N

H

S

L

A

G

K

H

D

K

L

S

H

C

L

V

E

Q

P

P

F

F

H

H

C

C

D

E

Y

F

G

G

Q

K

N

T

I

I

H

R

A

I

G

G

E

D

G

H

V

T

F

F

I

I

N

N

F

M

D

N

A

V

V

V

I

M

L

L

D

D

G

G

A

A

K

P

V

I

T

T

I

I

G

G

R

D

K

H

T

V

L

L

L

I

A

G

P

P

G

S

V

T

H

Q

I

F

Y

Y

T

T

A

A

R

S

H

H

P

S

L

L

N

D

V

Y

E

R

R

R

R

Q

E

A

W

W

E

E

D

T

-

I

C

C

R

K

P

P

V

I

T

V

I

I

G

E

E

D

C

V

W

W

I

I

G

G

H

N

V

V

T

V

I

I

C

N

P

Q

G
|
G

V

V

T

T

I

I

G

G

D

A

R

R

A

S

V

V

I

V

G

A

A

A

G

N

A

S

V

V

T

N

K

Q

D
|
D

I

V

P

P

A

P

D

D

S

T

L

L

A

V

V

G

G

G

N

T

P

P

A

A

K

R

V

I

I

L

K

R

T

S

L

L

N

K

E

D

binding calcium ion: G138 (= G142), D156 (= D160)

A1ADJ6 Polysialic acid O-acetyltransferase; Capsule O-acetyl transferase; EC 2.3.1.136 from Escherichia coli O1:K1 / APEC (see paper)
28% identity, 64% coverage: 56:176/189 of query aligns to 127:278/307 of A1ADJ6

query
sites
A1ADJ6

L

L

H

I

L

I

E

E

P

D

F

V

H

E

C

C

D

R

Y

W

G

L

Q

T

N

V

I

I

H

F

A

R

G

G

E

D

G

N

V

N

F

Y

I

V

N

R

F

I

-

H

-

K

-

N

-

S

-

K

-

I

-

K

-

G

D

D

A

I

V

V

I

A

L

T

D

K

G

G

A

S

K

K

V

V

T

I

I

I

G

G

R

R

K

R

T

T

L

T

L

I

A

G

P

A

G

G

V

F

H

E

I

V

Y

V

T

T

A

D

R

K

-

C

-

N

-

V

-

T

-

I

-

G

-

H

-

D

-

C

-

M

-

I

-

A

-

R

-

D

-

V

-

I

-

L

-

R

-

A

-

S

-

D

-

G

H
|
H

P

P

L

I

-

F

N

D

V

I

E

H

E

S

R

K

E

R

W

I

E

N

D

W

C

A

R

K

P

D

V

I

T

I

I

I

G

S

E

S

E

Y

C

V

W
|
W

I

V

G

G

G

R

H

N

V

V

T

S

I

I

C

M

P

K

G

G

V

V

T

S

I

V

G

G

D

S

R

G

A

S

V

V

I

I

G

G

A

Y

G

G

A

S

V

I

V

V

T

T

K

K

D

D

I

V

P

P

A

S

D

M

S

C

L

A

A

A

V

A

G

G

N

N

P

P

A

A

K

K

V

I

I

I

K

K

H210 (= H109) mutation to A: Loss of activity.
W234 (= W132) mutation to A: Loss of activity.

4husA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with virginiamycin m1 (see paper)
38% identity, 48% coverage: 87:176/189 of query aligns to 57:164/212 of 4husA

query
sites
4husA

G

G

A

D

K

K

V

L

T

I

I

I

G

G

R

R

K

F

T

C

L

S

L

I

A

G

P

P

G

G

V

T

H

T

I

F

Y

I

T

M

-

N

-

G

A

A

R

N

H

H

P

R

L

M

N

D

V

G

E

S

E

T

-

Y

-

P

-

F

-

H

-

L

-

F

R

R

R

M

E

G

W

W

E

E

D

K

C

Y

R

M

P

P

-

S

-

L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

-

D

V

I

T

E

I

I

G

G

E

N

E

D

C

V

W

W

I

I

G

G

G

R

H

D

V

V

T

T

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

I

G

A

A

A

G

E

A

A

V

V

T

T

K

K

D

N

I

V

P

A

A

P

D

Y

S

S

L

I

A

V

V

G

G

G

N

N

P

P

A

L

K

K

V

F

I

I

K

R

Sites not aligning to the query:

binding virginiamycin m1: 17, 36, 38

4hurA Crystal structure of streptogramin group a antibiotic acetyltransferase vata from staphylococcus aureus in complex with acetyl coenzyme a (see paper)
38% identity, 48% coverage: 87:176/189 of query aligns to 57:164/211 of 4hurA

query
sites
4hurA

G

G

A

D

K

K

V

L

T

I

I

I

G

G

R

R

K

F

T

C

L
x
S

L
x
I

A
x
G

P

P

G

G

V

T

H

T

I

F

Y

I

T

M

-

N

-

G

A
|
A

R
x
N

H

H

P

R

L

M

N

D

V

G

E

S

E

T

-

Y

-

P

-

F

-

H

-

L

-

F

R

R

R

M

E

G

W

W

E

E

D

K

C

Y

R

M

P

P

-

S

-

L

-

K

-

D

-

L

-

P

-

L

-
x
K

-

G

-

D

V

I

T

E

I

I

G

G

E

N

E

D

C

V

W
|
W

I
|
I

G
|
G

G
x
R

H

D

V

V

T

T

I

I

C
x
M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

I

G
x
A

A
|
A

G

E

A

A

V

V

T
|
T

K

K

D

N

I

V

P

A

A

P

D

Y

S

S

L

I

A

V

V
x
G

G

G

N

N

P
|
P

A

L

K

K

V

F

I
|
I

K
x
R

binding acetyl coenzyme *a: S67 (≠ L97), I68 (≠ L98), G69 (≠ A99), A79 (= A107), N80 (≠ R108), K110 (vs. gap), W120 (= W132), I121 (= I133), G122 (= G134), R123 (≠ G135), M128 (≠ C140), A140 (≠ G152), A141 (= A153), T146 (= T158), G156 (≠ V168), P159 (= P171), I163 (= I175), R164 (≠ K176)

Sites not aligning to the query:

binding acetyl coenzyme *a: 51

6x3jA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f0224 (46) (see paper)
38% identity, 48% coverage: 87:176/189 of query aligns to 57:164/206 of 6x3jA

query
sites
6x3jA

G

G

A

D

K

K

V

L

T

I

I

I

G

G

R

R

K

F

T

C

L

S

L

I

A
x
G

P

P

G

G

V

T

H

T

I

F

Y

I

T

M

-

N

-

G

A
|
A

R

N

H
|
H

P

R

L

M

N

D

V

G

E

S

E

T

-

Y

-

P

-

F

-
x
H

-
x
L

-

F

R

R

R

M

E

G

W

W

E

E

D

K

C

Y

R
x
M

P
|
P

-

S

-

L

-

K

-

D

-
x
L

-

P

-

L

-

K

-

G

-

D

V

I

T

E

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G
|
G

G

R

H

D

V

V

T

T

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

I

G

A

A

A

G

E

A

A

V

V

T

T

K

K

D

N

I

V

P

A

A

P

D

Y

S

S

L

I

A

V

V

G

G

G

N

N

P

P

A

L

K

K

V

F

I

I

K

R

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: H91 (vs. gap), L92 (vs. gap), M101 (≠ R123), P102 (= P124), L107 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A99), A79 (= A107), H81 (= H109), W120 (= W132), G122 (= G134)

Sites not aligning to the query:

binding (2R)-2-[(3S,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-4,12-dimethyl-1,7,22-trioxo-4,7,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,3H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosin-3-yl]propyl isoquinolin-3-ylcarbamate: 51, 53, 55

6x3cA Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
38% identity, 48% coverage: 87:176/189 of query aligns to 57:164/207 of 6x3cA

query
sites
6x3cA

G

G

A

D

K

K

V

L

T

I

I

I

G

G

R

R

K

F

T

C

L

S

L

I

A
x
G

P

P

G

G

V

T

H

T

I

F

Y

I

T

M

-

N

-
x
G

A

A

R
x
N

H
|
H

P

R

L

M

N

D

V

G

E

S

E

T

-

Y

-

P

-

F

-
x
H

-
x
L

-

F

R

R

R

M

E

G

W

W

E

E

D

K

C

Y

R
x
M

P
|
P

-

S

-
x
L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

-

D

V

I

T

E

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G
|
G

G
x
R

H

D

V

V

T

T

I

I

C
x
M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

I

G
x
A

A
|
A

G

E

A

A

V

V

T

T

K

K

D

N

I

V

P

A

A

P

D

Y

S

S

L

I

A

V

V

G

G

G

N

N

P

P

A

L

K

K

V

F

I

I

K

R

binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (vs. gap), N80 (≠ R108), H81 (= H109), H91 (vs. gap), L92 (vs. gap), M101 (≠ R123), P102 (= P124), L104 (vs. gap)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A99), N80 (≠ R108), H81 (= H109), W120 (= W132), G122 (= G134), R123 (≠ G135), M128 (≠ C140), A140 (≠ G152), A141 (= A153)

Sites not aligning to the query:

6x3cE Crystal structure of streptogramin a acetyltransferase vata from staphylococcus aureus in complex with streptogramin analog f1037 (47) (see paper)
38% identity, 48% coverage: 87:176/189 of query aligns to 57:164/203 of 6x3cE

query
sites
6x3cE

G

G

A

D

K

K

V

L

T

I

I

I

G

G

R

R

K

F

T

C

L

S

L

I

A
x
G

P

P

G

G

V

T

H

T

I

F

Y

I

T

M

-

N

-
x
G

A

A

R
x
N

H
|
H

P

R

L
x
M

N

D

V

G

E

S

E

T

-

Y

-

P

-

F

-
x
H

-
x
L

-

F

R

R

R

M

E

G

W

W

E

E

D

K

C

Y

R
x
M

P
|
P

-

S

-

L

-

K

-

D

-

L

-

P

-

L

-

K

-

G

-

D

V

I

T

E

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G
|
G

G

R

H

D

V

V

T

T

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

I

G
x
A

A
|
A

G

E

A

A

V

V

T

T

K

K

D

N

I

V

P

A

A

P

D

Y

S

S

L

I

A

V

V

G

G

G

N
|
N

P
|
P

A

L

K

K

V

F

I

I

K

R

binding magnesium ion: N158 (= N170), P159 (= P171)
binding (3R,4R,5E,10E,12E,14S,16R,26aR)-16-fluoro-14-hydroxy-12-methyl-3-(propan-2-yl)-4-(prop-2-en-1-yl)-3,4,8,9,14,15,16,17,24,25,26,26a-dodecahydro-1H,7H,22H-21,18-(azeno)pyrrolo[2,1-c][1,8,4,19]dioxadiazacyclotetracosine-1,7,22-trione: G78 (vs. gap), N80 (≠ R108), H81 (= H109), H91 (vs. gap), L92 (vs. gap), M101 (≠ R123), P102 (= P124)
binding thioacetic acid s-{2-[3-(2-hydroxy-3,3-dimethyl-4-phosphonooxy-butyrylamino)-propionylamino]-ethyl} ester: G69 (≠ A99), H81 (= H109), M83 (≠ L111), W120 (= W132), G122 (= G134), A140 (≠ G152), A141 (= A153)

Sites not aligning to the query:

1mrlA Crystal structure of streptogramin a acetyltransferase with dalfopristin (see paper)
36% identity, 50% coverage: 89:182/189 of query aligns to 60:171/204 of 1mrlA

query
sites
1mrlA

K

K

V

L

T

K

I

I

G

G

R

K

K

F

T

C

L

S

L

I

A

G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

-

N

-

G

-

A

-
x
N

-
x
H

-

R

-

M

-

D

-

G

A

S

R

T

H

Y

P

P

L

F

N

N

V
x
L

E

F

E

G

R

N

R

G

E

-

W

W

E

E

D

K

C

H

R
x
M

P

P

-

K

-

L

-

D

-

Q

-
x
L

-

P

-

I

-

K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W

W

I

I

G

G

G

K

H

D

V

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

V

G

A

A

A

G

N

A

S

V

V

T

V

K

K

D

D

I

I

P

A

A

P

D

Y

S

M

L

L

A

A

V

G

G

G

N

N

P

P

A

A

K

N

V

E

I

I

K

K

T

Q

L

R

N

F

E

D

K

Q

D

D

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: N81 (vs. gap), H82 (vs. gap), L93 (≠ V113), M102 (≠ R123), L108 (vs. gap)

Sites not aligning to the query:

binding 5-(2-diethylamino-ethanesulfonyl)-21-hydroxy-10-isopropyl-11,19-dimethyl-9,26-dioxa-3,15,28-triaza-tricyclo[23.2.1.00,255]octacosa-1(27),12,17,19,25(28)-pentaene-2,8,14,23-tetraone: 37, 39, 54, 56

1kk4A Crystal structure of vat(d) in complex with acetyl-coa (see paper)
36% identity, 50% coverage: 89:182/189 of query aligns to 60:171/205 of 1kk4A

query
sites
1kk4A

K

K

V

L

T

K

I

I

G

G

R

K

K

F

T

C

L

S

L
x
I

A
x
G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

-

N

-

G

-

A

-

N

-

H

-

R

-

M

-

D

-

G

A

S

R

T

H

Y

P

P

L

F

N

N

V

L

E

F

E

G

R

N

R

G

E

-

W

W

E

E

D

K

C

H

R

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-
x
K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G
|
G

G
x
K

H

D

V

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

V

G
x
A

A
|
A

G

N

A

S

V

V

T
x
V

K
|
K

D

D

I

I

P

A

A

P

D

Y

S

M

L
|
L

A

A

V
x
G

G
|
G

N

N

P
|
P

A

A

K

N

V

E

I
|
I

K

K

T

Q

L

R

N

F

E

D

K

Q

D

D

binding acetyl coenzyme *a: I69 (≠ L98), G70 (≠ A99), K111 (vs. gap), W121 (= W132), G123 (= G134), K124 (≠ G135), A141 (≠ G152), A142 (= A153), V147 (≠ T158), K148 (= K159), L155 (= L166), G157 (≠ V168), G158 (= G169), P160 (= P171), I164 (= I175)

3dhoA Structure of streptogramin acetyltransferase in complex with an inhibitor
36% identity, 50% coverage: 89:182/189 of query aligns to 60:171/203 of 3dhoA

query
sites
3dhoA

K

K

V

L

T

K

I

I

G

G

R

K

K

F

T

C

L

S

L

I

A

G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

-

N

-

G

-

A

-

N

-

H

-

R

-
x
M

-

D

-

G

A

S

R

T

H

Y

P

P

L

F

N

N

V

L

E

F

E

G

R

N

R

G

E

-

W

W

E

E

D

K

C

H

R

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-

K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W

W

I

I

G

G

G

K

H

D

V

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V
x
I

I

V

G
x
A

A

A

G

N

A

S

V

V

T
x
V

K

K

D

D

I

I

P

A

A

P

D

Y

S

M

L
|
L

A

A

V
x
G

G

G

N

N

P
|
P

A

A

K

N

V

E

I
|
I

K

K

T

Q

L

R

N

F

E

D

K

Q

D

D

binding 6-bromo-N'-[(1Z)-(3,5-dichloro-2-hydroxyphenyl)methylidene]-2-methylquinoline-4-carbohydrazide: M84 (vs. gap), I139 (≠ V150), A141 (≠ G152), V147 (≠ T158), L155 (= L166), G157 (≠ V168), P160 (= P171), I164 (= I175)

1khrA Crystal structure of vat(d) in complex with virginiamycin and coenzyme a (see paper)
36% identity, 50% coverage: 89:182/189 of query aligns to 60:171/206 of 1khrA

query
sites
1khrA

K

K

V

L

T

K

I

I

G

G

R

K

K

F

T

C

L

S

L

I

A

G

P

P

G

G

V

V

H

T

I

I

Y

I

T

M

-

N

-

G

-
x
A

-
x
N

-
x
H

-

R

-
x
M

-

D

-

G

A

S

R

T

H

Y

P

P

L

F

N

N

V
x
L

E

F

E

G

R

N

R

G

E

-

W

W

E

E

D

K

C

H

R

M

P

P

-

K

-

L

-

D

-

Q

-

L

-

P

-

I

-

K

-

G

-

D

V

T

T

I

I

I

G

G

E

N

E

D

C

V

W
|
W

I

I

G

G

G

K

H

D

V

V

T

V

I

I

C

M

P

P

G

G

V

V

T

K

I

I

G

G

D

D

R

G

A

A

V

I

I

V

G
x
A

A
|
A

G

N

A

S

V

V

T
x
V

K

K

D

D

I

I

P

A

A

P

D

Y

S

M

L
|
L

A

A

V
x
G

G

G

N

N

P
|
P

A

A

K

N

V

E

I
|
I

K

K

T

Q

L

R

N

F

E

D

K

Q

D

D

binding coenzyme a: H82 (vs. gap), M84 (vs. gap), W121 (= W132), A141 (≠ G152), A142 (= A153), V147 (≠ T158), L155 (= L166), G157 (≠ V168), P160 (= P171), I164 (= I175)
binding virginiamycin m1: A80 (vs. gap), N81 (vs. gap), H82 (vs. gap), L93 (≠ V113)

Sites not aligning to the query:

binding virginiamycin m1: 37, 39, 54

Query Sequence

>Echvi_3679 FitnessBrowser__Cola:Echvi_3679
MEKMMAGEPYDAHCEDMIRIRTRVKRILHKLNVTEYYTDKFQATINELCPNSAKDLHLEP
PFHCDYGQNIHAGEGVFINFDAVILDGAKVTIGRKTLLAPGVHIYTARHPLNVEERREWE
DCRPVTIGEECWIGGHVTICPGVTIGDRAVIGAGAVVTKDIPADSLAVGNPAKVIKTLNE
KDHKTTYKT

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory