SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing Echvi_3767 FitnessBrowser__Cola:Echvi_3767 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 13 hits to proteins with known functional sites (download)

3dr2A Structural and functional analyses of xc5397 from xanthomonas campestris: a gluconolactonase important in glucose secondary metabolic pathways (see paper)
30% identity, 88% coverage: 31:333/344 of query aligns to 10:288/299 of 3dr2A

query
sites
3dr2A

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binding calcium ion: E45 (= E70), N131 (= N172), N188 (= N224), D236 (= D281)

3e5zA X-ray structure of the putative gluconolactonase in protein family pf08450. Northeast structural genomics consortium target drr130.
28% identity, 85% coverage: 50:342/344 of query aligns to 10:282/290 of 3e5zA

query
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3e5zA

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binding magnesium ion: E30 (= E70), N117 (= N172), N174 (= N224), D220 (= D281)

3o4pA Dfpase at 0.85 angstrom resolution (h atoms included) (see paper)
24% identity, 78% coverage: 69:337/344 of query aligns to 20:289/314 of 3o4pA

query
sites
3o4pA

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active site: E21 (= E70), E37 (vs. gap), N120 (= N172), N175 (= N224), D229 (= D281), H287 (= H335)
binding calcium ion: E21 (= E70), N120 (= N172), N175 (= N224), D229 (= D281), D232 (≠ K284), L273 (≠ C321), H274 (≠ T322)
binding 1,2-dimethoxyethane: W244 (vs. gap), K269 (≠ L317), H287 (= H335)
binding 2-methoxyethanol: K151 (vs. gap), T169 (≠ S219), A170 (vs. gap), F171 (≠ L220), E194 (≠ N239), W201 (≠ F248), E212 (≠ D259), N213 (≠ T260), K214 (≠ R261), K214 (≠ R261)

Sites not aligning to the query:

binding 1,2-dimethoxyethane: 8, 296, 297

Q7SIG4 Diisopropyl-fluorophosphatase; DFPase; EC 3.1.8.2 from Loligo vulgaris (Common European squid) (see 4 papers)
24% identity, 78% coverage: 69:337/344 of query aligns to 20:289/314 of Q7SIG4

query
sites
Q7SIG4

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E21 (= E70) mutation to Q: 100% decrease in activity. Loss of calcium 1 binding.
E37 (vs. gap) mutation to Q: 50% decrease in activity.
Q77 (≠ L125) mutation to F: 100% decrease in activity.; mutation to W: No effect on activity.; mutation to Y: 6% increase in activity.
N120 (= N172) mutation to D: 96% decrease in activity. 100% decrease in activity; when associated with N-229.
D121 (= D173) mutation to F: 100% decrease in activity.
Y144 (≠ A196) mutation to S: 8% increase in activity.
R146 (≠ K198) mutation to S: 45% decrease in activity.
M148 (≠ L200) mutation to A: 26% decrease in activity.
F173 (≠ R222) mutation to A: 84% decrease in activity.; mutation to L: 28% decrease in activity.; mutation to S: 68% decrease in activity.; mutation to V: 46% decrease in activity.; mutation to W: 19% decrease in activity.; mutation to Y: 53% decrease in activity.
N175 (= N224) mutation to D: 98% decrease in activity.
H181 (≠ P230) mutation to N: 20% decrease in activity.
T195 (≠ S240) mutation to A: 60% decrease in activity.; mutation to L: 11% decrease in activity.; mutation to V: 3% decrease in activity.
H219 (≠ D266) mutation to N: 3% increase in activity.
H224 (≠ R276) mutation to N: 14% increase in activity.
D229 (= D281) mutation to N: 100% decrease in activity. Loss of calcium 1 binding. 100% decrease in activity; when associated with D-120.
D232 (≠ K284) binding ; mutation to S: 3% increase in activity. 19% decrease in activity; when associated with A-271.
N237 (≠ G289) mutation to S: 4% decrease in activity.
W244 (vs. gap) mutation to F: 44% decrease in activity.; mutation to H: 27% decrease in activity.; mutation to L: 62% decrease in activity.; mutation to Y: No effect on activity.
H248 (vs. gap) mutation to N: 4% increase in activity.
S271 (= S319) mutation to A: 30% increase in activity. 19% decrease in activity; when associated with S-232.
N272 (= N320) mutation to F: 100% decrease in activity.
L273 (≠ C321) binding
H274 (≠ T322) binding ; mutation to N: 85% decrease in activity.
H287 (= H335) active site, Proton acceptor; mutation to A: 90% decrease in activity.; mutation to F: 36% decrease in activity.; mutation to L: 21% decrease in activity.; mutation to N: 97% decrease in activity.; mutation to Q: 54% decrease in activity.; mutation to W: 44% decrease in activity.; mutation to Y: 57% decrease in activity.

Sites not aligning to the query:

304 Q→F: 50% decrease in activity.; Q→W: 3% decrease in activity.
314 F→A: 3% increase in activity.

2gvvA Structure of diisopropyl fluorophosphatase (dfpase) in complex with dicyclopentylphosphoroamidate (dcppa) (see paper)
24% identity, 78% coverage: 69:337/344 of query aligns to 18:287/309 of 2gvvA

query
sites
2gvvA

V

A

E
|
E

G

G

P

P

L

V

W

F

I

D

G

K

K

N

G

G

D

D

Y

F

L

Y

L

I

F

V

S

A

-
x
P

-
x
E

-

V

-

E

-

V

-

N

D

G

I

K

P

P

R

A

N

G

K

E

V

I

Y

L

K

R

M

I

T

D

S

L

E

K

G

T

D

G

T

K

S

K

T

T

Y

V

L

I

N

C

R

K

S

P

G

E

Y

V

S

N

G

G

E

Y

G

G

A

G

Y

I

S

P

R

A

E

-

P

-

G

-

S

-

N

-

A

G

L

C

L

Q

L

C

D

D

K

R

D

D

-

A

N

N

Q

Q

L

L

V

F

L

-

M

-

Q

-

H

-

G

-

N

-

R

-

K

-

V

V

A

A

K

D

M

M

K

R

G

L

G

G

L

L

D

L

A

V

P

V

A

Q

P

T

D

D

-

G

-

T

F

F

T

E

S

E

L

I

V

A

H

K

-

K

D

D

Y

S

Q

E

G

G

K

R

R

R

L

M

N

Q

S

G

P

C

N
|
N

D

D

G

C

I

A

F

F

D

D

K

Y

Q

E

G

G

N

N

L

L

Y

W

F

I

T

T

D

A

P

P

P

A

Y

G

G

E

L

V

P

A

P

P

R

A

F

D

A

Y

G

T

K

R

E

S

L

M

S

Q

-

E

-

K

F

F

Q

G

G

S

I

I

Y

Y

C

C

L

F

K

T

T

T

N

D

G

G

T

Q

L

M

V

I

L

Q

L

V

D

D

S

T

-

A

L

F

S

Q

R

F

P

P

N
|
N

G

G

I

I

A

A

L

V

S

R

P

H

D

M

E

N

K

D

-

G

-

R

-

P

-

Y

H

Q

L

L

F

I

V

V

A

A

N

E

S

T

D

P

E

T

K

K

N

K

A

L

A

-

W

-

F

W

Q

S

Y

Y

P

D

L

I

E

K

A

G

P

P

G

A

E

K

V

I

D

E

T

N

R

K

S

K

L

V

F

W

Y

G

D

H

A

-

T

-

E

-

V

-

L

I

P

P

G

G

G

T

R

H

N

E

G

G

L

G

P

A

D
|
D

G

G

M

M

K
x
D

V

F

H

D

P

E

K

D

G

N

Y

N

L

L

F

L

A

V

T

A

-

N

-
x
W

-

G

-

S

-

H

-

I

-

E

-

V

-

F

G

G

P

P

D

D

G

G

I

-

W

-

I

-

F

-

D

-

L

-

K

-

G

G

K

Q

V

P

L

K

G

M

K

R

I

I

H

R

T

C

G

P

-

F

Q

E

L

K

T

P

S

S

N

N

C
x
L

T
x
H

F

F

T

K

D

P

D

Q

Y

T

K

K

H

T

L

I

Y

F

V

V

T

T

A

E

H
|
H

R

E

D

N

active site: E19 (= E70), E35 (vs. gap), N118 (= N172), N173 (= N224), D227 (= D281), H285 (= H335)
binding calcium ion: E19 (= E70), N118 (= N172), N173 (= N224), D227 (= D281), D230 (≠ K284), L271 (≠ C321), H272 (≠ T322)
binding dicyclopentyl phosphoramidate: E19 (= E70), P34 (vs. gap), N118 (= N172), N173 (= N224), D227 (= D281), W242 (vs. gap), H285 (= H335)

Q9A9Z1 D-xylonolactone lactonase; Xylono-1,5-lactonase; EC 3.1.1.110 from Caulobacter vibrioides (strain ATCC 19089 / CB15) (Caulobacter crescentus) (see paper)
25% identity, 78% coverage: 70:336/344 of query aligns to 17:254/289 of Q9A9Z1

query
sites
Q9A9Z1

E
|
E

G

G

P

P

L

I

W

W

I

-

G

-

K

H

G

G

D

D

Y

T

L

L

L

W

F

F

S

V

D

D

I

I

P

K

R

Q

N

R

K

K

V

I

Y

H

K

-

M

-

T

-

S

-

E

-

G

-

D

-

T

-

S

-

T

-

Y

-

L

-

N

N

R

Y

S

H

G

P

Y

A

S

T

G

G

E

E

G

R

A

F

Y

S

S

F

R

D

E

A

P

P

G

-

S

-

N

-

A

-

L

-

D

D

K

Q

D

V

N

T

Q

F

L

L

V

A

L

P

M

I

Q

V

H

G

G

A

N

T

R

G

K

F

V

V

A

V

K

G

M

L

K

K

G

T

G

G

L

I

D

H

A

R

-

F

-

H

P

P

A

A

P

T

D

G

F

F

T

S

S

L

L

L

V

L

H

E

D

V

Y

E

Q

D

G

A

K

A

R

L

L

N

N

N

S

R

P

P

N

N

D

D

G

A

I

T

F

V

D

D

K

A

Q

Q

G

G

N

R

L

L

Y

W

F

F

T

G

D

T

P

-

Y

-

G

-

L

-

P

-

R

M

F

H

A

D

G

G

K

E

E

E

L

N

S

N

F

S

Q

G

G

S

I

L

Y

Y

C

R

L

M

K

D

T

L

N

T

G

G

T

V

L

A

V

R

L

M

L

D

D

R

S

D

L

I

S

C

R

I

P

T

N
|
N

G

G

I

P

A

C

L

V

S

S

P

P

D

D

E

G

K

K

H

T

L

F

F

Y

V

H

A

T

N

D

S

T

D

L

E

E

K

K

N

T

A

I

A

-

W

-

F

Y

Q

A

Y

F

P

D

L

L

E

A

A

E

P

D

G

G

E

L

V

L

D

S

T

N

R

K

S

R

L

V

F

F

Y

V

D

Q

A

-

T

-

E

-

V

-

L

F

P

A

G

L

G

G

R

D

N

D

G

V

L

Y

P

P

D
|
D

G

G

M

S

K

V

V

V

H

D

P

S

K

E

G

G

Y

Y

L

L

F

W

A

T

T

A

-

L

-

W

G

G

P

G

D

F

G

G

I

A

W

V

I

R

F

F

D

S

L

P

K

Q

G

G

K

D

V

A

L

V

G

T

K

R

I

I

H

E

-

L

-

P

T

A

G

P

Q

N

L

V

T

T

S

K

N

P

C

C

T

F

F

G

T

G

D

P

D

D

Y

L

K

K

H

T

L

L

Y

Y

V

F

T

T

A

T

H

A

R

R

E17 (= E70) binding
N145 (= N224) binding
D195 (= D281) binding

7plbB Caulobacter crescentus xylonolactonase with d-xylose (see paper)
25% identity, 78% coverage: 70:336/344 of query aligns to 17:254/289 of 7plbB

query
sites
7plbB

E
|
E

G

G

P

P

L

I

W

W

I

-

G

-

K
x
H

G

G

D

D

Y

T

L

L

L

W

F

F

S

V

D

D

I

I

P

K

R

Q

N

R

K

K

V

I

Y

H

K

-

M

-

T

-

S

-

E

-

G

-

D

-

T

-

S

-

T

-

Y

-

L

-

N

N

R

Y

S

H

G

P

Y

A

S

T

G

G

E

E

G

R

A

F

Y

S

S

F

R

D

E

A

P

P

G

-

S

-

N

-

A

-

L

-

D

D

K

Q

D

V

N

T

Q

F

L

L

V

A

L

P

M

I

Q

V

H

G

G
x
A

N
x
T

R
x
G

K
x
F

V

V

A

V

K

G

M

L

K

K

G

T

G

G

L

I

D

H

A

R

-

F

-
x
H

P
|
P

A

A

P

T

D

G

F

F

T

S

S

L

L

L

V

L

H
x
E

D
x
V

Y
x
E

Q
x
D

G

A

K

A

R

L

L

N

N

N

S
x
R

P

P

N
|
N

D

D

G

A

I

T

F

V

D

D

K

A

Q

Q

G

G

N
x
R

L

L

Y

W

F

F

T

G

D

T

P

-

Y

-

G

-

L

-

P

-

R
x
M

F

H

A

D

G

G

K
x
E

E

E

L

N

S

N

F

S

Q

G

G

S

I

L

Y

Y

C
x
R

L

M

K
x
D

T

L

N
x
T

G

G

T

V

L

A

V

R

L

M

L

D

D

R

S

D

L

I

S

C

R

I

P

T

N
|
N

G

G

I

P

A

C

L

V

S

S

P

P

D

D

E

G

K

K

H

T

L

F

F

Y

V

H

A

T

N

D

S

T

D

L

E

E

K

K

N

T

A

I

A

-

W

-

F

Y

Q

A

Y

F

P

D

L

L

E

A

A

E

P
x
D

G

G

E

L

V

L

D

S

T

N

R

K

S

R

L

V

F

F

Y

V

D

Q

A

-

T

-

E

-

V

-

L

F

P

A

G

L

G

G

R

D

N

D

G

V

L

Y

P

P

D
|
D

G

G

M

S

K

V

V

V

H

D

P

S

K

E

G

G

Y

Y

L

L

F

W

A

T

T

A

-

L

-
x
W

G

G

P

G

D

F

G

G

I

A

W

V

I

R

F

F

D

S

L

P

K

Q

G

G

K

D

V

A

L

V

G

T

K

R

I

I

H

E

-

L

-

P

T

A

G

P

Q

N

L

V

T

T

S

K

N

P

C

C

T

F

F

G

T

G

D

P

D

D

Y

L

K

K

H

T

L

L

Y

Y

V

F

T

T

A

T

H

A

R

R

binding fe (ii) ion: E17 (= E70), N100 (= N172), N145 (= N224), D195 (= D281)
binding beta-D-xylopyranose: E17 (= E70), E89 (≠ H161), V90 (≠ D162), E91 (≠ Y163), D92 (≠ Q164), R98 (≠ S170), N100 (= N172), R109 (≠ N181), M115 (≠ R194), E119 (≠ K198), R128 (≠ C207), D130 (≠ K209), T132 (≠ N211), N145 (= N224), D174 (≠ P255), D195 (= D281), W210 (vs. gap)
binding alpha-D-xylopyranose: H22 (≠ K77), A64 (≠ G138), T65 (≠ N139), G66 (≠ R140), F67 (≠ K141), H79 (vs. gap), P80 (= P152)

Sites not aligning to the query:

binding beta-D-xylopyranose: 15

7pldA Caulobacter crescentus xylonolactonase with (r)-4-hydroxy-2- pyrrolidone (see paper)
25% identity, 78% coverage: 70:336/344 of query aligns to 15:252/287 of 7pldA

query
sites
7pldA

E
|
E

G

G

P

P

L

I

W

W

I

-

G

-

K

H

G

G

D

D

Y

T

L

L

L

W

F

F

S

V

D

D

I

I

P

K

R

Q

N

R

K

K

V

I

Y

H

K

-

M

-

T

-

S

-

E

-

G

-

D

-

T

-

S

-

T

-

Y

-

L

-

N

N

R

Y

S

H

G

P

Y

A

S

T

G

G

E

E

G

R

A

F

Y

S

S

F

R

D

E

A

P

P

G

-

S

-

N

-

A

-

L

-

D

D

K

Q

D

V

N

T

Q

F

L

L

V

A

L

P

M

I

Q

V

H

G

G

A

N

T

R

G

K

F

V

V

A

V

K

G

M

L

K

K

G

T

G

G

L

I

D

H

A

R

-

F

-

H

P

P

A

A

P

T

D

G

F

F

T

S

S

L

L

L

V

L

H

E

D

V

Y

E

Q

D

G

A

K

A

R

L

L

N

N

N

S

R

P

P

N
|
N

D

D

G

A

I

T

F

V

D

D

K

A

Q

Q

G

G

N

R

L

L

Y

W

F

F

T

G

D

T

P

-

Y

-

G

-

L

-

P

-

R

M

F

H

A

D

G

G

K
x
E

E

E

L

N

S

N

F

S

Q

G

G

S

I

L

Y

Y

C

R

L

M

K

D

T

L

N

T

G

G

T

V

L

A

V

R

L

M

L

D

D

R

S

D

L

I

S

C

R

I

P

T

N
|
N

G

G

I

P

A

C

L

V

S

S

P

P

D

D

E

G

K

K

H

T

L

F

F

Y

V

H

A

T

N

D

S
x
T

D
x
L

E

E

K

K

N

T

A

I

A

-

W

-

F

Y

Q

A

Y

F

P

D

L

L

E

A

A

E

P

D

G

G

E

L

V

L

D

S

T

N

R

K

S

R

L

V

F

F

Y

V

D

Q

A

-

T

-

E

-

V

-

L

F

P

A

G

L

G

G

R

D

N

D

G

V

L

Y

P

P

D
|
D

G

G

M

S

K

V

V

V

H

D

P

S

K

E

G

G

Y

Y

L

L

F

W

A

T

T

A

-

L

-
x
W

G

G

P

G

D

F

G

G

I

A

W

V

I

R

F

F

D

S

L

P

K

Q

G

G

K

D

V

A

L

V

G

T

K

R

I

I

H

E

-

L

-

P

T

A

G

P

Q

N

L

V

T

T

S

K

N

P

C

C

T

F

F

G

T

G

D

P

D

D

Y

L

K

K

H

T

L

L

Y

Y

V

F

T

T

A

T

H

A

R

R

binding (R)-4-hydroxy-2-pyrrolidone: E117 (≠ K198), T159 (≠ S240), L160 (≠ D241), D193 (= D281), W208 (vs. gap)
binding fe (ii) ion: E15 (= E70), N98 (= N172), N143 (= N224), D193 (= D281)

Sites not aligning to the query:

binding (R)-4-hydroxy-2-pyrrolidone: 253

7rizA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound 2-hydroxyquinoline (see paper)
25% identity, 80% coverage: 59:333/344 of query aligns to 1:282/306 of 7rizA

query
sites
7rizA

I

V

E

R

R

V

M

L

A

A

S

T

G

D

F

L

D

A

W
x
F

V

P

E
|
E

G

G

P

P

L

V

W

V

I

M

G

P

K

D

G

G

D

S

Y

V

L

V

L

L

F

V

S

-

D

E

I

I

P

R

R

A

N

Q

K

Q

V

L

Y

T

K

R

M

V

T

W

S

P

E

D

G

G

D

R

T

K

S

E

T

V

Y

V

L

A

N

K

R

V

S

P

G

G

Y

-

S

-

G

-

E

-

G

-

A

-

Y

-

S

-

R

-

E

-

P

-

G

G

S

P

N
|
N

A

G

L

A

L

L

D

G

K

P

D

D

N

G

Q

K

L

M

V

Y

L

I

M

C

Q

N

H

N

G

G

N

G

R
x
F

K

G

V

W

A

F

K

P

M

S

K

R

G

G

G

T

L

M

-

M

-
x
P

D

G

A

A

P

P

A

A

P

P

-

H

-

E

-

Y

-

I

-

G

-

S

-

I

-

Q

-

R

-

V

-

D

-

L

-

Q

-

S

-

G

D

E

F

V

T

E

S

T

L

L

V

F

H

D

D

K

Y

C

Q

G

G

E

K

H

R

P

L

L

N

K

S

G

P

P

N
|
N

D

D

G

L

I

V

F

F

D

D

K

K

Q

H

G

G

N

G

L

L

Y

W

F

F

T

T

D

D

P

-

Y

-

G

-

L
|
L

P

G

P

K

R

R

F

R

A

A

G

-

K

R

E

D

L

M

S

D

F

V

Q

G

G

A

I

A

Y

Y

C

Y

L

I

K

K

T

P

N

G

G

M

T

T

L

E

V

I

-

T

-

E

-

Q

L

V

L

F

D

G

S

T

L

L

S

P

R

L

P

-

N
|
N

G

G

I

I

A

G

L

L

S

S

P

P

D

D

E

E

K

A

H

T

L

M

F

Y

V

A

A

A

N

E

S

T

D

-

E

-

K

P

N

T

A

G

A

R

W

L

F

W

Q

A

Y

F

P

D

L

L

E

S

A

G

P

P

G

G

E

E

V

V

D

K

T

P

R

R

S

D

L

V

F

I

Y

Y

D

R

A

G

T

E

E

K

E

G

V

K

L

P

P

I

G

C

G

G

R

L

N

G

G

G

-

Y

-

Q

L

M

P

F

D
|
D

G
x
S

M

L

K

A

V

V

H

E

P

A

K

S

G

G

-

N

Y

V

L

C

F

V

A

A

T

T

G
x
L

P

V

D

S

G

G

-

C

I

I

W

S

I

V

F

I

D

A

L

P

K

D

G

G

K

T

V

L

L

V

G

E

K

Q

I

V

H

P

T

T

G

G

-

D

Q

R

L

V

T

T

S

T

N

N

C

I

T

A

F

F

T

G

D

G

-

P

D

D

Y

L

K

K

H

T

L

A

Y

Y

V

I

T

T

binding calcium ion: E12 (= E70), N120 (= N172), N169 (= N224), D226 (= D281), S227 (≠ G282)
binding quinolin-2(1h)-one: F10 (≠ W68), E12 (= E70), N52 (= N122), F70 (≠ R140), P81 (vs. gap), N120 (= N172), L135 (= L191), N169 (= N224), D226 (= D281), L241 (≠ G295)

7risA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound phosphate (see paper)
30% identity, 48% coverage: 168:333/344 of query aligns to 107:269/293 of 7risA

query
sites
7risA

L

L

N

K

S

G

P

P

N
|
N

D

D

G

L

I

V

F

F

D

D

K

K

Q

H

G

G

N

G

L

L

Y

W

F

F

T

T

D

D

P

-

Y

-

G

-

L

L

P

G

P

K

R

R

F

R

A

A

G

-

K

R

E

D

L

M

S

D

F

V

Q

G

G

A

I

A

Y

Y

C

Y

L

I

K

K

T

P

N

G

G

M

T

T

L

E

V

I

-

T

-

E

-

Q

L

V

L

F

D

G

S

T

L

L

S

P

R

L

P

-

N
|
N

G

G

I

I

A

G

L

L

S

S

P

P

D

D

E

E

K

A

H

T

L

M

F

Y

V

A

A

A

N

E

S

T

D

-

E

-

K

P

N

T

A

G

A

R

W

L

F

W

Q

A

Y

F

P

D

L

L

E

S

A

G

P

P

G

G

E

E

V

V

D

K

T

P

R

R

S

D

L

-

F

-

Y

G

D

E

A

K

T

G

E

K

E

P

V

I

L

C

P

G

G

L

G

G

R

G

N

Y

G

Q

L

M

P

F

D
|
D

G
x
S

M

L

K

A

V

V

H

E

P

A

K

S

G

G

-

N

Y

V

L

C

F

V

A

A

T

T

G

L

P

V

D

S

G

G

-

C

I

I

W

S

I

V

F

I

D

A

L

P

K

D

G

G

K

T

V

L

L

V

G

E

K

Q

I

V

H

P

T

T

G

G

-

D

Q

R

L

V

T

T

S
x
T

N

N

C

I

T

A

F

F

T

G

D

G

-

P

D

D

Y

L

K

K

H

T

L

A

Y

Y

V

I

T

T

binding calcium ion: N111 (= N172), N160 (= N224), D213 (= D281), S214 (≠ G282)
binding phosphate ion: N111 (= N172), N160 (= N224), D213 (= D281), T254 (≠ S319)

Sites not aligning to the query:

8dk0A Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound (s)gamma- valerolactone (see paper)
29% identity, 48% coverage: 168:333/344 of query aligns to 111:269/293 of 8dk0A

query
sites
8dk0A

L

L

N

K

S

G

P

P

N
|
N

D

D

G

L

I

V

F

F

D

D

K

K

Q

H

G

G

N

G

L

L

Y

W

F

F

T

T

D

D

P

-

Y

-

G

-

L

L

P

G

P

K

R

R

F

R

A

A

G

-

K

R

E

D

L

M

S

D

F

V

Q

G

G

A

I

A

Y

Y

C

Y

L

I

K

K

T

P

N

G

G

M

T

T

L

E

V

I

-

T

-

E

-

Q

L

V

L

F

D

G

S

T

L

L

S

P

R

L

P

-

N
|
N

G

G

I

I

A

G

L

L

S

S

P

P

D

D

E

E

K

A

H

T

L

M

F

Y

V

A

A

A

N

E

S

T

D

-

E

-

K

P

N

T

A

G

A

R

W

L

F

W

Q

A

Y

F

P

D

L

L

E

S

A

G

P

P

G

G

E

E

V

V

D

K

T

P

R

K

S

G

L

-

F

-

Y

-

D

-

A

-

T

-

E

K

E

P

V

I

L

C

P

G

G

L

G

G

R

G

N

Y

G

Q

L

M

P

F

D
|
D

G
x
S

M

L

K

A

V

V

H

E

P

A

K

S

G

G

-

N

Y

V

L

C

F

V

A

A

T

T

G

L

P

V

D

S

G

G

-

C

I

I

W

S

I

V

F

I

D

A

L

P

K

D

G

G

K

T

V

L

L

V

G

E

K

Q

I

V

H

P

T

T

G

G

-

D

Q

R

L

V

T

T

S
x
T

N

N

C

I

T

A

F

F

T

G

D

G

-

P

D

D

Y

L

K

K

H

T

L

A

Y

Y

V

I

T

T

binding calcium ion: N115 (= N172), N164 (= N224), D213 (= D281), S214 (≠ G282)
binding gamma-Valerolactone: D213 (= D281), T254 (≠ S319)

Sites not aligning to the query:

8djzA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound product (see paper)
29% identity, 48% coverage: 168:333/344 of query aligns to 111:269/293 of 8djzA

query
sites
8djzA

L

L

N

K

S

G

P

P

N
|
N

D

D

G

L

I

V

F

F

D

D

K

K

Q

H

G

G

N

G

L

L

Y

W

F

F

T

T

D

D

P

-

Y

-

G

-

L

L

P

G

P

K

R

R

F

R

A

A

G

-

K

R

E

D

L

M

S

D

F

V

Q

G

G

A

I

A

Y

Y

C

Y

L

I

K

K

T

P

N

G

G

M

T

T

L

E

V

I

-

T

-

E

-

Q

L

V

L

F

D

G

S

T

L

L

S

P

R

L

P

-

N
|
N

G

G

I

I

A

G

L

L

S

S

P

P

D

D

E

E

K

A

H

T

L

M

F

Y

V

A

A

A

N

E

S

T

D

-

E

-

K

P

N

T

A

G

A

R

W

L

F

W

Q

A

Y

F

P

D

L

L

E

S

A

G

P

P

G

G

E

E

V

V

D

K

T

P

R

K

S

G

L

-

F

-

Y

-

D

-

A

-

T

-

E

K

E

P

V

I

L

C

P

G

G

L

G

G

R

G

N

Y

G

Q

L

M

P

F

D
|
D

G
x
S

M

L

K

A

V

V

H

E

P

A

K

S

G

G

-

N

Y

V

L

C

F

V

A

A

T

T

G

L

P

V

D

S

G

G

-

C

I

I

W

S

I

V

F

I

D

A

L

P

K

D

G

G

K

T

V

L

L

V

G

E

K

Q

I

V

H

P

T

T

G

G

-

D

Q

R

L

V

T

T

S
x
T

N

N

C

I

T

A

F

F

T

G

D

G

-

P

D

D

Y

L

K

K

H

T

L

A

Y

Y

V

I

T

T

binding calcium ion: N115 (= N172), N164 (= N224), D213 (= D281), S214 (≠ G282)
binding (4R)-4-hydroxypentanoic acid: N115 (= N172), N164 (= N224), D213 (= D281), T254 (≠ S319)

Sites not aligning to the query:

8djfA Crystal structure of rpa3624, a beta-propeller lactonase from rhodopseudomonas palustris, with active-site bound tetrahedral intermediate (see paper)
29% identity, 48% coverage: 168:333/344 of query aligns to 111:269/293 of 8djfA

query
sites
8djfA

L

L

N

K

S

G

P

P

N
|
N

D

D

G

L

I

V

F

F

D

D

K

K

Q

H

G

G

N

G

L

L

Y

W

F

F

T

T

D

D

P

-

Y

-

G

-

L

L

P

G

P

K

R

R

F

R

A

A

G

-

K

R

E

D

L

M

S

D

F

V

Q

G

G

A

I

A

Y

Y

C

Y

L

I

K

K

T

P

N

G

G

M

T

T

L

E

V

I

-

T

-

E

-

Q

L

V

L

F

D

G

S

T

L

L

S

P

R

L

P

-

N
|
N

G

G

I

I

A

G

L

L

S

S

P

P

D

D

E

E

K

A

H

T

L

M

F

Y

V

A

A

A

N

E

S

T

D

-

E

-

K

P

N

T

A

G

A

R

W

L

F

W

Q

A

Y

F

P

D

L

L

E

S

A

G

P

P

G

G

E

E

V

V

D

K

T

P

R

K

S

G

L

-

F

-

Y

-

D

-

A

-

T

-

E

K

E

P

V

I

L

C

P

G

G

L

G

G

R

G

N

Y

G

Q

L

M

P

F

D
|
D

G
x
S

M

L

K

A

V

V

H

E

P

A

K

S

G

G

-

N

Y

V

L

C

F

V

A

A

T

T

G

L

P

V

D

S

G

G

-

C

I

I

W

S

I

V

F

I

D

A

L

P

K

D

G

G

K

T

V

L

L

V

G

E

K

Q

I

V

H

P

T

T

G

G

-

D

Q

R

L

V

T

T

S
x
T

N

N

C

I

T

A

F

F

T

G

D

G

-

P

D

D

Y

L

K

K

H

T

L

A

Y

Y

V

I

T

T

binding calcium ion: N115 (= N172), N164 (= N224), D213 (= D281), S214 (≠ G282)
binding (5S)-5-methyloxolane-2,2-diol: N115 (= N172), N164 (= N224), D213 (= D281), T254 (≠ S319)

Sites not aligning to the query:

Query Sequence

>Echvi_3767 FitnessBrowser__Cola:Echvi_3767
MNLKPMLIGWLLVTAFGCSDQQKTSQVSKHAIPTSSEKPFSIEILDDQALKVISRDATIE
RMASGFDWVEGPLWIGKGDYLLFSDIPRNKVYKMTSEGDTSTYLNRSGYSGEGAYSREPG
SNALLLDKDNQLVLMQHGNRKVAKMKGGLDAPAPDFTSLVHDYQGKRLNSPNDGIFDKQG
NLYFTDPPYGLPPRFAGKELSFQGIYCLKTNGTLVLLDSLSRPNGIALSPDEKHLFVANS
DEKNAAWFQYPLEAPGEVDTRSLFYDATEEVLPGGRNGLPDGMKVHPKGYLFATGPDGIW
IFDLKGKVLGKIHTGQLTSNCTFTDDYKHLYVTAHRDILRVDLK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory