SitesBLAST
Comparing Echvi_3769 FitnessBrowser__Cola:Echvi_3769 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q6BF17 D-galactonate dehydratase; GalD; EC 4.2.1.6 from Escherichia coli (strain K12)
55% identity, 99% coverage: 5:386/386 of query aligns to 1:382/382 of Q6BF17
- H185 (= H189) mutation H->N,Q: Loss of activity.
- H285 (= H289) mutation to N: Loss of activity.
- E310 (= E314) mutation to Q: Loss of activity.
3rraB Crystal structure of enolase prk14017 (target efi-500653) from ralstonia pickettii 12j with magnesium bound
55% identity, 99% coverage: 5:386/386 of query aligns to 2:375/379 of 3rraB
- active site: I35 (= I38), R38 (≠ K41), Y118 (= Y121), K145 (= K148), N147 (= N150), E151 (= E154), D184 (= D187), H186 (= H189), E210 (= E213), G235 (= G238), E236 (= E239), R237 (= R240), Q257 (= Q260), D259 (= D262), H286 (= H289), P288 (= P291), E311 (= E314)
- binding magnesium ion: D184 (= D187), E210 (= E213), E236 (= E239)
4e4fB Crystal structure of enolase pc1_0802 (target efi-502240) from pectobacterium carotovorum subsp. Carotovorum pc1
35% identity, 99% coverage: 5:386/386 of query aligns to 2:381/381 of 4e4fB
- active site: L37 (≠ I38), R40 (≠ K41), R148 (≠ K148), Q150 (≠ N150), D189 (= D187), H191 (= H189), E215 (= E213), G240 (= G238), E241 (= E239), V242 (≠ R240), R262 (≠ Q260), T264 (≠ D262), H291 (= H289), P293 (= P291), E318 (= E314), W381 (= W386)
- binding magnesium ion: D189 (= D187), E215 (= E213), E241 (= E239)
Q1NAJ2 D-mannonate dehydratase; ManD; EC 4.2.1.8 from Sphingomonas sp. (strain SKA58) (see paper)
33% identity, 97% coverage: 12:386/386 of query aligns to 10:403/403 of Q1NAJ2
- D211 (= D187) binding
- E237 (= E213) binding
- E263 (= E239) binding
3thuA Crystal structure of an enolase from sphingomonas sp. Ska58 (efi target efi-501683) with bound mg
33% identity, 97% coverage: 12:386/386 of query aligns to 12:405/405 of 3thuA
- active site: L39 (≠ I38), E43 (≠ A42), W79 (≠ Y77), G124 (≠ C122), R150 (≠ K148), Q152 (≠ N150), Y162 (vs. gap), D213 (= D187), H215 (= H189), E239 (= E213), G264 (= G238), E265 (= E239), R286 (≠ Q260), T288 (≠ D262), H315 (= H289), E342 (= E314), W405 (= W386)
- binding magnesium ion: D213 (= D187), E239 (= E213), E265 (= E239)
4fi4A Crystal structure of mannonate dehydratase prk15072 (target efi- 502214) from caulobacter sp. K31
32% identity, 99% coverage: 5:386/386 of query aligns to 6:407/407 of 4fi4A
- active site: L41 (≠ I38), R44 (≠ K41), H127 (≠ W123), R152 (≠ K148), Q154 (vs. gap), Y164 (vs. gap), S187 (= S159), D215 (= D187), H217 (= H189), E241 (= E213), G266 (= G238), E267 (= E239), I268 (≠ R240), R288 (≠ Q260), T290 (≠ D262), C316 (≠ P288), H317 (= H289), G318 (vs. gap), A319 (vs. gap), E344 (= E314), W407 (= W386)
- binding magnesium ion: D215 (= D187), E241 (= E213), E267 (= E239)
B0T0B1 D-mannonate dehydratase Caul1427; ManD; EC 4.2.1.8 from Caulobacter sp. (strain K31) (see paper)
32% identity, 99% coverage: 5:386/386 of query aligns to 2:403/403 of B0T0B1
- D211 (= D187) binding
- E237 (= E213) binding
- E263 (= E239) binding
3rgtA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with d-arabinohydroxamate
34% identity, 95% coverage: 19:386/386 of query aligns to 17:383/383 of 3rgtA
- active site: H122 (≠ W123), R147 (≠ K148), Q149 (≠ N150), D191 (= D187), H193 (= H189), E217 (= E213), G242 (= G238), E243 (= E239), R264 (≠ Q260), P266 (≠ D262), H293 (= H289), G294 (≠ C290), E320 (= E314), W383 (= W386)
- binding cobalt (ii) ion: D191 (= D187), E217 (= E213), E243 (= E239)
- binding (2S,3R,4R)-2,3,4,5-tetrahydroxy-N-oxo-pentanamide: N37 (≠ E39), D191 (= D187), H193 (= H189), E243 (= E239), H293 (= H289), P295 (= P291), D297 (vs. gap), E320 (= E314), W383 (= W386)
3p93C Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
34% identity, 95% coverage: 19:386/386 of query aligns to 17:384/384 of 3p93C
- active site: H122 (≠ W123), R147 (≠ K148), Q149 (≠ N150), D192 (= D187), H194 (= H189), E218 (= E213), G243 (= G238), E244 (= E239), R265 (≠ Q260), P267 (≠ D262), H294 (= H289), G295 (≠ C290), E321 (= E314), W384 (= W386)
- binding 2-keto-3-deoxygluconate: N37 (≠ E39), D192 (= D187), H194 (= H189), E244 (= E239), H294 (= H289), P296 (= P291), D298 (vs. gap), E321 (= E314), W384 (= W386)
- binding magnesium ion: D192 (= D187), E218 (= E213), E244 (= E239)
3p93A Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg,d-mannonate and 2-keto-3-deoxy-d- gluconate
34% identity, 95% coverage: 19:386/386 of query aligns to 17:384/384 of 3p93A
- active site: H122 (≠ W123), R147 (≠ K148), Q149 (≠ N150), D192 (= D187), H194 (= H189), E218 (= E213), G243 (= G238), E244 (= E239), R265 (≠ Q260), P267 (≠ D262), H294 (= H289), G295 (≠ C290), E321 (= E314), W384 (= W386)
- binding d-mannonic acid: N37 (≠ E39), D192 (= D187), H194 (= H189), E244 (= E239), H294 (= H289), P296 (= P291), D298 (vs. gap), E321 (= E314)
- binding magnesium ion: D192 (= D187), E218 (= E213), E244 (= E239)
3v3wA Crystal structure of an enolase from the soil bacterium cellvibrio japonicus (target efi-502161) with bound mg and glycerol
31% identity, 95% coverage: 19:386/386 of query aligns to 17:397/397 of 3v3wA
- active site: L36 (≠ I38), R39 (≠ K41), H122 (≠ W123), K144 (≠ T145), R147 (≠ K148), Q149 (≠ N150), Y159 (vs. gap), E179 (≠ V161), D205 (= D187), H207 (= H189), E231 (= E213), G256 (= G238), E257 (= E239), V258 (≠ R240), R278 (≠ Q260), T280 (≠ D262), F306 (≠ P288), H307 (= H289), G308 (vs. gap), A309 (vs. gap), E334 (= E314), W397 (= W386)
- binding magnesium ion: D205 (= D187), E231 (= E213), E257 (= E239)
4kwsA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol
33% identity, 95% coverage: 19:386/386 of query aligns to 17:397/397 of 4kwsA
- active site: H122 (≠ W123), R147 (≠ K148), Q149 (≠ N150), Y159 (vs. gap), D205 (= D187), H207 (= H189), E231 (= E213), G256 (= G238), E257 (= E239), R278 (≠ Q260), P280 (≠ D262), H307 (= H289), G308 (≠ C290), E334 (= E314), W397 (= W386)
- binding magnesium ion: D205 (= D187), E231 (= E213), E257 (= E239)
3qkeA Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and d-gluconate
33% identity, 95% coverage: 19:386/386 of query aligns to 17:397/397 of 3qkeA
- active site: H122 (≠ W123), R147 (≠ K148), Q149 (≠ N150), Y159 (vs. gap), D205 (= D187), H207 (= H189), E231 (= E213), G256 (= G238), E257 (= E239), R278 (≠ Q260), P280 (≠ D262), H307 (= H289), G308 (≠ C290), E334 (= E314), W397 (= W386)
- binding D-gluconic acid: N37 (≠ E39), Y159 (vs. gap), D205 (= D187), H207 (= H189), E257 (= E239), H307 (= H289), P309 (= P291), D311 (vs. gap), E334 (= E314), W397 (= W386)
- binding magnesium ion: D205 (= D187), E231 (= E213), E257 (= E239)
B3PDB1 D-galactonate dehydratase family member RspA; D-mannonate dehydratase; Starvation sensing protein RspA homolog; EC 4.2.1.-; EC 4.2.1.8 from Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa) (see paper)
31% identity, 95% coverage: 19:386/386 of query aligns to 17:402/402 of B3PDB1
- D210 (= D187) binding
- E236 (= E213) binding
- E262 (= E239) binding
Q1QT89 D-galactonate dehydratase family member ManD; D-gluconate dehydratase; D-mannonate dehydratase; EC 4.2.1.-; EC 4.2.1.39; EC 4.2.1.8 from Chromohalobacter salexigens (strain ATCC BAA-138 / DSM 3043 / CIP 106854 / NCIMB 13768 / 1H11) (see paper)
32% identity, 95% coverage: 19:386/386 of query aligns to 17:403/403 of Q1QT89
- D211 (= D187) binding
- E237 (= E213) binding
- E263 (= E239) binding
- H313 (= H289) mutation to N: Abolishes activity with D-gluconate and D-mannonate.; mutation to Q: Abolishes activity with D-gluconate. No effect on activity with D-mannonate.
- P315 (= P291) mutation to A: Strongly increases activity with D-mannonate. Slightly decreases activity with D-gluconate.
2qq6B Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from rubrobacter xylanophilus dsm 9941
32% identity, 85% coverage: 32:359/386 of query aligns to 31:381/396 of 2qq6B
- active site: P37 (≠ I38), G79 (= G80), D124 (vs. gap), K166 (= K148), D168 (≠ N150), D213 (= D187), H215 (= H189), E239 (= E213), G264 (= G238), E265 (= E239), M286 (≠ Q260), D288 (= D262), H315 (= H289), N316 (≠ C290), E340 (≠ Q313), D345 (≠ G318)
- binding magnesium ion: D213 (= D187), E239 (= E213), E265 (= E239), H315 (= H289)
4e6mA Crystal structure of putative dehydratase protein from salmonella enterica subsp. Enterica serovar typhimurium (salmonella typhimurium)
30% identity, 93% coverage: 5:363/386 of query aligns to 2:394/401 of 4e6mA
- active site: L45 (≠ I38), G48 (≠ K41), K169 (= K148), D171 (vs. gap), I175 (vs. gap), E223 (≠ D187), H225 (= H189), E249 (= E213), G274 (= G238), E275 (= E239), R276 (= R240), Q296 (= Q260), D298 (= D262), H325 (= H289), C327 (≠ P291), E350 (= E314), A355 (≠ I319)
- binding magnesium ion: E223 (≠ D187), E249 (= E213), E275 (= E239)
3rcyF Crystal structure of mandelate racemase/muconate lactonizing enzyme- like protein from roseovarius sp. Tm1035
32% identity, 88% coverage: 19:358/386 of query aligns to 23:371/397 of 3rcyF
- active site: K165 (= K148), D167 (≠ N150), R175 (vs. gap), G208 (≠ D187), H210 (= H189), E234 (= E213), G259 (= G238), E260 (= E239), Q281 (= Q260), A283 (≠ D262), H310 (= H289), A313 (≠ L292), L332 (≠ F311), E335 (= E314)
- binding magnesium ion: E234 (= E213), E260 (= E239)
- binding alpha-D-ribofuranose: R85 (= R78), P86 (≠ G79), P239 (≠ A218), A266 (≠ W245), E267 (≠ D246)
3gy1B Crystal structure of putative mandelate racemase/muconate lactonizing protein from clostridium beijerinckii ncimb 8052
30% identity, 89% coverage: 20:361/386 of query aligns to 23:360/388 of 3gy1B
- active site: R152 (≠ K148), Q154 (≠ N150), D191 (= D187), H193 (= H189), E217 (= E213), G242 (= G238), E243 (= E239), R264 (≠ Q260), H266 (≠ D262), H293 (= H289), E320 (= E314)
- binding magnesium ion: D191 (= D187), E217 (= E213), E243 (= E239)
4ihcB Crystal structure of probable mannonate dehydratase dd703_0947 (target efi-502222) from dickeya dadantii ech703
32% identity, 92% coverage: 5:358/386 of query aligns to 2:367/395 of 4ihcB
- active site: F39 (≠ I38), R42 (≠ K41), R150 (≠ K148), Q152 (vs. gap), D201 (vs. gap), H203 (= H189), E227 (= E213), G252 (= G238), E253 (= E239), L254 (≠ R240), R274 (≠ Q260), H276 (≠ D262), H303 (= H289), P305 (= P291), E330 (= E314)
- binding magnesium ion: D201 (vs. gap), E227 (= E213), E253 (= E239)
Sites not aligning to the query:
Query Sequence
>Echvi_3769 FitnessBrowser__Cola:Echvi_3769
MSNDLKIERIELFKVPPRWLFVKITTKSGLVGWGEPVIEGKADTVAACVREMEQYLIGRG
AHEIEDIWQVLYRGGFYRGGPILMSALSGIDQALWDIKGKHLNVPVYELLGGAVRQKMKM
YCWIGGDHPEVVLEQAQEKVDAGYTAVKMNATGEMDWVSSVKEVKKVVENIKLIRQHFGD
SLDVGLDFHGRVHKPMVKRLIDELSPFDPLFIEEPVLAENNDALGHIYRYSAIPIATGER
MFSRWDFKEILHQGVVDIIQPDLSHAGGISEVRRIATMAEAYDITIAPHCPLGPISLASA
LHVDFVSANAFIQESSLGIHYNQGFDLLDYVKNPEVFDLKEGYIDLFDRPGLGVEMDEER
LKEGQKIGHHWANPIWRNADGSFAEW
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory