SitesBLAST
Comparing Echvi_3846 FitnessBrowser__Cola:Echvi_3846 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 19 hits to proteins with known functional sites (download)
4xfjB Crystal structure of argininosuccinate synthase from mycobacterium thermoresistibile in complex with amppnp and arginine
32% identity, 97% coverage: 2:388/399 of query aligns to 2:390/397 of 4xfjB
- active site: D13 (= D13), R94 (= R95), D123 (= D124), S174 (= S174)
- binding phosphoaminophosphonic acid-adenylate ester: A7 (= A7), Y8 (= Y8), S9 (= S9), T14 (= T14), I34 (≠ V34), G116 (= G117), C117 (≠ S118), F127 (= F128)
- binding arginine: Y86 (= Y87), S90 (≠ V91), R126 (= R127), A183 (≠ S183), E185 (vs. gap), E259 (≠ G256), E269 (≠ V266), Y271 (≠ F268)
7k5zA Crystal structure of argininosuccinate synthase from legionella pneumophila philadelphia 1 in complex with anppnp and a substrate analogue arginine
33% identity, 99% coverage: 1:394/399 of query aligns to 3:385/390 of 7k5zA
- active site: D15 (= D13), R95 (= R95), D124 (= D124), S176 (= S174)
- binding phosphoaminophosphonic acid-adenylate ester: A9 (= A7), Y10 (= Y8), S11 (= S9), C37 (≠ V34), G117 (= G117), F128 (= F128)
- binding arginine: Y88 (= Y87), T92 (≠ S92), D124 (= D124), R127 (= R127), S185 (= S183), E187 (≠ G185), E261 (≠ G256), Y273 (≠ F268)
P59846 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see 2 papers)
27% identity, 95% coverage: 3:381/399 of query aligns to 2:382/400 of P59846
- 6:14 (vs. 7:15, 89% identical) binding
- A33 (≠ V34) binding
- G114 (= G117) binding
2nz2A Crystal structure of human argininosuccinate synthase in complex with aspartate and citrulline (see paper)
28% identity, 97% coverage: 4:392/399 of query aligns to 4:400/402 of 2nz2A
- active site: D13 (= D13), R92 (= R95), D121 (= D124), S176 (= S174)
- binding aspartic acid: A115 (≠ S118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124)
- binding citrulline: Y84 (= Y87), T88 (≠ V91), N120 (= N123), R124 (= R127), D178 (≠ N176), S185 (= S183), E187 (≠ G185), E266 (≠ G256), Y278 (≠ F268)
P00966 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Homo sapiens (Human) (see 16 papers)
28% identity, 97% coverage: 4:392/399 of query aligns to 7:405/412 of P00966
- V64 (= V64) to I: in CTLN1; uncertain significance; dbSNP:rs556297791
- Y87 (= Y87) binding
- T91 (≠ V91) to P: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs769018733
- S92 (= S92) binding
- R95 (= R95) to S: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity
- P96 (≠ I96) to H: in CTLN1; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; to L: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; loss of argininosuccinate synthase activity; to S: in CTLN1; no effect on thermal stability; decreased argininosuccinate synthase activity
- G117 (= G117) to S: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770944877
- A118 (≠ S118) to T: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs775305020
- T119 (= T119) binding ; to I: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity
- N123 (= N123) binding ; binding
- D124 (= D124) binding ; to N: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs936192871
- R127 (= R127) binding ; to L: increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to Q: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs201623252; to W: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs771794639
- R157 (= R152) to C: in CTLN1; decreased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs770585183; to H: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908637
- K165 (= K160) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-176 or R-176.
- K176 (= K170) modified: N6-acetyllysine; by CLOCK; mutation K->Q,R: Significant loss of acetylation but no decrease in enzyme activity; when associated with Q-165 or R-165.
- W179 (≠ Y173) to R: in CTLN1; mild; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908646
- S180 (= S174) binding ; to I: in CTLN1; increased thermal stability; loss of argininosuccinate synthase activity; dbSNP:rs121908638; to N: in CTLN1; decreased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908638
- S189 (= S183) binding
- E191 (≠ G185) to Q: in CTLN1; loss of argininosuccinate synthase activity
- A192 (≠ G186) to V: in CTLN1; decreased protein abundance
- V263 (≠ I249) to M: in CTLN1; mild clinical course; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs192838388
- R265 (= R251) to C: in CTLN1; severe clinical course; loss of argininosuccinate synthase activity; dbSNP:rs148918985
- E270 (≠ G256) binding ; to Q: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs775163147
- R272 (≠ T258) to C: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs762387914; to H: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008; to L: in CTLN1; increased thermal stability; decreased affinity for aspartate; decreased affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs768215008
- G280 (≠ V266) to R: in CTLN1; loss of argininosuccinate synthase activity
- Y282 (≠ F268) binding
- T284 (≠ A270) to I: in CTLN1; mild clinical course; dbSNP:rs886039853
- M302 (≠ L288) to V: in CTLN1; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity
- R304 (≠ K290) to W: in CTLN1; decreased protein abundance; dbSNP:rs121908642
- G324 (= G310) to S: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908639
- G347 (= G333) to R: in CTLN1; severe clinical course
- Y359 (≠ Q345) to D: in CTLN1; mild clinical course
- G362 (= G348) to V: in CTLN1; mild; no effect on affinity for aspartate; no effect on affinity for citrulline; decreased argininosuccinate synthase activity; dbSNP:rs121908647
- G390 (= G377) to R: in CTLN1; loss of argininosuccinate synthase activity; dbSNP:rs121908641
1j20A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with product (see paper)
28% identity, 95% coverage: 3:381/399 of query aligns to 2:373/386 of 1j20A
- active site: D12 (= D13), R92 (= R95), D121 (= D124), S168 (≠ E165)
- binding adenosine monophosphate: A6 (= A7), T13 (= T14), A33 (≠ V34), R92 (= R95), H113 (= H116), G114 (= G117), F125 (= F128)
- binding argininosuccinate: Y84 (= Y87), T88 (≠ V91), A115 (≠ S118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124), R124 (= R127), S177 (= S174), E179 (≠ N176), E253 (≠ G256), Y265 (≠ F268)
1j1zA Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with substrate (see paper)
28% identity, 95% coverage: 3:381/399 of query aligns to 2:373/386 of 1j1zA
- active site: D12 (= D13), R92 (= R95), D121 (= D124), S168 (≠ E165)
- binding aspartic acid: A115 (≠ S118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124)
- binding adenosine-5'-triphosphate: A6 (= A7), T13 (= T14), A33 (≠ V34), R92 (= R95), I95 (≠ Q98), H113 (= H116), G114 (= G117), F125 (= F128)
- binding citrulline: Y84 (= Y87), T88 (≠ V91), R124 (= R127), S168 (≠ E165), M169 (= M166), S177 (= S174), E179 (≠ N176), E253 (≠ G256), Y265 (≠ F268)
1kh3A Crystal structure of thermus thermophilus hb8 argininosuccinate synthetase in complex with inhibitor (see paper)
27% identity, 95% coverage: 3:381/399 of query aligns to 2:367/380 of 1kh3A
- active site: D12 (= D13), R92 (= R95), D121 (= D124), S168 (≠ E165)
- binding phosphoaminophosphonic acid-adenylate ester: A6 (= A7), T13 (= T14), T32 (≠ L33), A33 (≠ V34), H113 (= H116), G114 (= G117), F125 (= F128), S168 (≠ E165), M169 (= M166)
- binding arginine: Y84 (= Y87), T88 (≠ V91), R124 (= R127), S168 (≠ E165), M169 (= M166), D170 (≠ N167), S177 (= S174), E179 (≠ N176), E253 (≠ G256), Y265 (≠ F268)
- binding aspartic acid: A115 (≠ S118), T116 (= T119), G119 (= G122), N120 (= N123), D121 (= D124)
6e5yA 1.50 angstrom resolution crystal structure of argininosuccinate synthase from bordetella pertussis in complex with amp.
25% identity, 85% coverage: 2:340/399 of query aligns to 11:364/438 of 6e5yA
5us8A 2.15 angstrom resolution crystal structure of argininosuccinate synthase from bordetella pertussis
25% identity, 85% coverage: 2:340/399 of query aligns to 15:368/445 of 5us8A
P0A6E4 Argininosuccinate synthase; Citrulline--aspartate ligase; EC 6.3.4.5 from Escherichia coli (strain K12) (see 4 papers)
25% identity, 85% coverage: 2:340/399 of query aligns to 12:365/447 of P0A6E4
- 17:25 (vs. 7:15, 78% identical) binding
- A43 (≠ V34) binding
- Y99 (= Y87) binding
- G129 (= G117) binding
- T131 (= T119) binding ; binding
- N135 (= N123) binding ; binding
- D136 (= D124) binding ; binding
- R139 (= R127) binding
- S192 (= S174) binding
- D194 (≠ N176) binding
- T201 (≠ S183) binding
- E203 (≠ G185) binding
- E280 (≠ G256) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1k97A Crystal structure of e. Coli argininosuccinate synthetase in complex with aspartate and citrulline (see paper)
25% identity, 85% coverage: 2:340/399 of query aligns to 11:364/432 of 1k97A
- active site: D22 (= D13), R106 (= R95), D135 (= D124), S191 (= S174)
- binding aspartic acid: S129 (= S118), T130 (= T119), G133 (= G122), N134 (= N123), D135 (= D124)
- binding citrulline: Y98 (= Y87), T102 (≠ V91), P103 (≠ S92), R138 (= R127), S191 (= S174), T192 (≠ I175), D193 (≠ N176), T200 (≠ S183), E202 (≠ G185), E279 (≠ G256), Y291 (≠ F268), Y331 (≠ H308)
1kp3A Crystal structure of e. Coli argininosuccinate synthetase in complex with atp and citrulline (see paper)
25% identity, 85% coverage: 2:340/399 of query aligns to 11:364/439 of 1kp3A
- active site: D22 (= D13), R106 (= R95), D135 (= D124), S191 (= S174)
- binding adenosine-5'-triphosphate: A16 (= A7), S18 (= S9), G20 (= G11), D22 (= D13), T23 (= T14), T41 (≠ L33), A42 (≠ V34), D127 (≠ H116), G128 (= G117), S129 (= S118), F139 (= F128), D193 (≠ N176)
- binding citrulline: Y98 (= Y87), T102 (≠ V91), P103 (≠ S92), T130 (= T119), G133 (= G122), N134 (= N123), D135 (= D124), R138 (= R127), D193 (≠ N176), T200 (≠ S183), E202 (≠ G185), E202 (≠ G185), E279 (≠ G256), S287 (≠ G264), Y291 (≠ F268)
2pg3A Crystal structure of a queuosine biosynthesis protein quec (eca1155) from erwinia carotovora subsp. Atroseptica scri1043 at 2.40 a resolution
33% identity, 29% coverage: 1:117/399 of query aligns to 1:113/221 of 2pg3A
Sites not aligning to the query:
Q6D820 7-cyano-7-deazaguanine synthase; 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein QueC; EC 6.3.4.20 from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia carotovora subsp. atroseptica)
31% identity, 29% coverage: 1:117/399 of query aligns to 1:122/231 of Q6D820
Sites not aligning to the query:
- 188 binding
- 197 binding
- 200 binding
- 203 binding
8cp3A Apo-lare in complex with amp-pnp (see paper)
28% identity, 38% coverage: 2:152/399 of query aligns to 20:152/245 of 8cp3A
- binding phosphoaminophosphonic acid-adenylate ester: A25 (= A7), Y26 (= Y8), S27 (= S9), G29 (= G11), D31 (= D13), S32 (≠ T14), I50 (≠ V34), F55 (= F39), D119 (≠ H116), G120 (= G117), R130 (vs. gap), F136 (≠ L136)
8cp4B [4fe-4s] cluster containing lare in complex with amp (see paper)
28% identity, 38% coverage: 2:152/399 of query aligns to 17:149/254 of 8cp4B
Sites not aligning to the query:
8cp4A [4fe-4s] cluster containing lare in complex with amp (see paper)
28% identity, 38% coverage: 2:152/399 of query aligns to 18:150/255 of 8cp4A
Sites not aligning to the query:
8cnzD Mmlare-[4fe-4s] phased by fe-sad (see paper)
28% identity, 38% coverage: 2:152/399 of query aligns to 24:156/261 of 8cnzD
Sites not aligning to the query:
Query Sequence
>Echvi_3846 FitnessBrowser__Cola:Echvi_3846
MKKVVLAYSGGLDTTFCAIHLSQEKGYEVHAVLVNTGGFSEEELKTTEERAGKLGIASFK
VLDVTQTYYNEVIKYLIFGNVLKNQTYPLSVSAERILQAKALAEYAKQIGAKAVAHGSTG
AGNDQVRFDMIFQTILPEAEIITPIRDLQLSREAEIEFLKKHGVEMNFEKAKYSINKGIW
GTSVGGKETLTSGDTLPEEAYPTQLTKETPTQISLQFEAGELVGVNGQKFSNPVDAILKV
QALADPYAIGRDIHVGDTIIGIKGRVGFEAAAPLIIIKAHQLLEKHTLTKWQTFWKNQLS
EFYGNHLHEGHYLDPVMRNLEAFLADTQQFVSGEVKVALKPYQFQLIGIDSPHDLMSAKF
GSYGEMNKGYTAEDVKGFTKILGNQTAIYHKVNQTLDND
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory