SitesBLAST

P24058 Argininosuccinate lyase; ASAL; Arginosuccinase; Delta crystallin II; Delta-2 crystallin; EC 4.3.2.1 from Anas platyrhynchos (Mallard) (Anas boschas) (see 4 papers)
32% identity, 87% coverage: 2:388/444 of query aligns to 9:394/468 of P24058

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P24058

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W11 (= W4) mutation to A: 98% decrease in catalytic efficiency.; mutation to F: 90% decrease in catalytic efficiency.; mutation to M: 99% decrease in catalytic efficiency.; mutation to R: 97% decrease in catalytic efficiency.; mutation to Y: 50% decrease in catalytic efficiency.
S29 (≠ D22) binding in chain A; mutation to A: 10% decrease in catalytic efficiency.
D33 (= D26) mutation to N: 99% decrease in catalytic efficiency.
D89 (= D82) mutation to N: Loss of activity.
N116 (= N109) binding in chain A; mutation to D: 99% decrease in catalytic efficiency.
D117 (= D110) mutation to A: 55% decrease in catalytic efficiency.; mutation to E: 58% decrease in catalytic efficiency.
T161 (= T154) binding in chain C; mutation to A: Loss of activity.; mutation to D: Loss of activity.; mutation to S: 30% decrease in catalytic efficiency.; mutation to V: Loss of activity.
H162 (= H155) mutation to E: Loss of activity.
R238 (= R231) mutation to Q: Loss of activity.
T281 (= T275) mutation to V: 80% decrease in catalytic efficiency.
S283 (= S277) mutation to A: Loss of activity.; mutation to C: Loss of activity.; mutation to D: Loss of activity.; mutation to H: Loss of activity.; mutation to T: Loss of activity.
N291 (= N285) binding in chain B; mutation to L: Loss of activity.
D293 (= D287) mutation to N: 99% decrease in catalytic efficiency.
E296 (= E290) mutation to D: Loss of activity.
Y323 (= Y317) binding in chain A
K325 (≠ R319) mutation to N: 99% decrease in catalytic efficiency.
Q328 (= Q322) binding in chain A
D330 (≠ L324) mutation to N: Loss of activity.
K331 (= K325) binding in chain A; mutation to Q: Loss of activity.

1k7wD Crystal structure of s283a duck delta 2 crystallin mutant (see paper)
33% identity, 82% coverage: 25:388/444 of query aligns to 15:377/450 of 1k7wD

query
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active site: E71 (= E81), T144 (= T154), H145 (= H155), A266 (≠ S277), S267 (= S278), K272 (= K283), E279 (= E290)
binding argininosuccinate: R98 (= R108), N99 (= N109), V102 (= V112), T144 (= T154), H145 (= H155), Y306 (= Y317), Q311 (= Q322), K314 (= K325)

P04424 Argininosuccinate lyase; ASAL; Arginosuccinase; EC 4.3.2.1 from Homo sapiens (Human) (see 12 papers)
33% identity, 82% coverage: 25:388/444 of query aligns to 30:392/464 of P04424

query
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P04424

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D31 (= D26) to N: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs754995756
K51 (≠ S46) mutation to N: 2-fold reduction in activity.
K69 (≠ D64) modified: N6-acetyllysine
E73 (= E68) to K: in ARGINSA; complete loss of argininosuccinate lyase activity; abolishes protein expression
D87 (= D82) to G: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs752100894
H89 (= H84) mutation to Q: 10-fold reduction in activity.
R94 (≠ F89) to C: in ARGINSA; severe; dbSNP:rs374304304
R95 (≠ L90) to C: in ARGINSA; loss of argininosuccinate lyase activity; dbSNP:rs28940585
R113 (= R108) to Q: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on nitric oxide production; dbSNP:rs752783461
D120 (= D115) to E: in ARGINSA; severe
V178 (≠ E173) to M: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941473
T181 (≠ A176) to S: in a breast cancer sample; somatic mutation
R182 (≠ E177) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs751590073
R186 (≠ L181) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs752397242
G200 (= G195) to V: in a breast cancer sample; somatic mutation
R236 (= R231) to W: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; no effect on NOS complex formation; dbSNP:rs761268464
D237 (≠ G232) to N: in ARGINSA; severe; dbSNP:rs552951774
Q286 (≠ H282) to R: in ARGINSA; complete loss of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs28941472
K288 (= K284) modified: N6-acetyllysine; mutation to R: Refractory to inhibition by TSA and NAM and by addition of extra amino acids. No effect on protein structure.
R297 (= R293) to Q: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression; dbSNP:rs750431938
R306 (≠ G302) to W: in ARGINSA; severe; dbSNP:rs868834862
Q326 (= Q322) to L: in ARGINSA; severe
V335 (≠ D331) to L: in ARGINSA; reduction of argininosuccinate lyase activity; no effect on protein expression
M360 (≠ L356) to T: in ARGINSA; loss of argininosuccinate lyase activity; may cause protein misfolding; dbSNP:rs875989948
M382 (= M378) to R: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression
R385 (= R381) to L: in ARGINSA; severe
H388 (≠ Y384) to Q: in ARGINSA; severe

Sites not aligning to the query:

12 R → Q: in ARGINSA; 18-fold reduction in catalytic efficiency toward argininosuccinate; dbSNP:rs145138923
398 A → D: in ARGINSA; impairs tetramer formation likely due to protein misfolding; loss of argininosuccinate lyase activity
456 R → W: in ARGINSA; reduction of argininosuccinate lyase activity; reduces protein expression; dbSNP:rs759396688

1hy0A Crystal structure of wild type duck delta 1 crystallin (eye lens protein) (see paper)
31% identity, 81% coverage: 29:388/444 of query aligns to 17:375/447 of 1hy0A

query
sites
1hy0A

L

L

A

S

P

E

F

V

D

D

V

I

L

Q

G

A

S

S

L

I

A

A

H

Y

A

A

T

K

M

A

L

L

E

E

S

K

I

A

D

G

L

I

L

L

T

T

K

K

E

T

E

E

L

L

A

E

I

K

L

I

K

L

K

S

G

G

L

L

K

E

D

K

I

I

Y

S

Q

E

E

E

I

L

Q

S

E

K

G

G

T

V

F

I

T

V

I

V

D

T

P

Q

G

S

V

D

E
|
E

D

D

V

I

H

Q

S

T

Q

A

V

N

E

E

F

R

L

R

L

L

T

K

E

E

R

L

Y

I

G

G

D

D

V

I

G

A

K

G

K

K

L

L

H

H

S

T

G

G

R

R

S

S

R

R

N

N

D

E

Q

Q

V

V

A

V

V

T

D

D

L

L

K

K

L

L

Y

F

Y

M

R

K

A

N

V

S

I

L

Q

S

E

I

V

I

L

-

D

-

A

S

K

T

A

H

L

L

F

L

D

Q

L

L

L

I

L

K

T

T

L

L

A

V

E

E

K

R

H

A

Q

A

N

I

D

E

-

I

-

D

-

V

L

I

M

L

P

P

G

G

Y

Y

T
|
T

H
|
H

T

L

Q

Q

L

K

A

A

M

Q

P

P

S

I

S

R

F

W

G

S

L

Q

W

F

F

L

G

L

A

S

M

H

A

A

E

V

S

A

L

L

A

T

E

R

D

D

M

S

E

E

L

R

W

L

L

G

A

E

A

V

Y

K

N

K

L

R

A

I

D

N

R

V

N

L

P

P

L

L

G

G

S

S

A

G

A

A

G

L

Y

A

G

G

S

N

S

P

F

L

P

D

L

I

N

D

R

R

T

E

M

M

T

L

T

R

R

S

L

E

L

L

G

E

F

F

K

A

D

S

M

I

H

S

Y

L

N

N

V

S

I

M

N

D

A

A

Q

I

N

S

N

E

R

R

G

D

K

F

T

V

E

V

K

E

A

F

I

L

A

S

F

V

A

A

M

T

A

L

G

L

M

L

A

I

G

H

T

-

L

L

N

S

R

K

L

M

S

A

A

E

D

D

I

L

I

I

F

Y

M

S

N

T

Q

S

H

E

F

F

G

G

F

F

V

L

K

T

F

L

P

S

D

D

N

A

L

F

T

S

T

T

G

G

S
|
S

I

L

M
|
M

P

P

H

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

S

F

L

E
|
E

L

L

I

I

R

R

A

S

K

K

A

S

N

G

Q

R

I

V

Q

F

S

G

G

R

P

L

Q

A

N

S

L

I

M

L

M

M

Q

V

M

L

T

K

N

G

T

L

T

P

T

S

G

T

Y

Y

H

N

R

K

D

D

L

L

Q

Q

L

E

L

D

K

K

E

E

T

A

T

V

F

I

P

D

D

V

F

V

E

D

K

T

L

L

K

T

D

A

C

V

L

L

Q

Q

I

V

T

A

K

T

F

G

M

V

L

I

E

S

H

T

I

L

E

Q

I

I

K

S

P

K

D

E

I

N

L

M

E

E

D

K

K

A

F

L

Y

T

K

P

H

E

L

M

F

L

S

A

V

T

E

D

V

L

V

A

N

L

D

Y

L

L

V

V

L

R

Q

K

G

G

M

M

P

P

F

F

R

R

D

Q

A

A

Y

H

K

T

K

A

V

S

G

G

active site: E69 (= E81), T142 (= T154), H143 (= H155), S264 (= S277), S265 (= S278), K270 (= K283), E277 (= E290)
binding sulfate ion: S264 (= S277), M267 (= M280), N272 (= N285)

P02521 Delta-1 crystallin; Delta crystallin I from Gallus gallus (Chicken) (see paper)
32% identity, 84% coverage: 16:388/444 of query aligns to 12:392/466 of P02521

query
sites
P02521

Q

R

F

F

T

V

I

G

G

S

R

T

D

D

P

P

E

I

F

M

D

E

I

I

V

L

-

S

-

I

-

S

-

T

-

E

-

Q

-

R

L

L

A

T

P

E

F

V

D

D

V

I

L

Q

G

A

S

S

L

M

A

A

H

Y

A

A

T

K

M

A

L

L

E

E

S

K

I

A

D

S

L

I

L

L

T

T

K

K

E

T

E

E

L

L

A

E

I

K

L

I

K

L

K

S

G

G

L

L

K

E

D

K

I

I

Y

S

Q

E

E

E

I

S

Q

S

E

K

G

G

T

V

F

L

T

V

I

M

D

T

P

Q

G

S

V

D

E

E

D

D

V

I

H

Q

S

T

Q

A

V

I

E

E

F

R

L

R

L

L

T

K

E

E

R

L

Y

I

G

G

D

D

V

I

G

A

K

G

K

K

L

L

H

Q

S

T

G

G

R

R

S

S

R

R

N

N

D

E

Q

Q

V

V

A

V

V

T

D

D

L

L

K

K

L

L

Y

L

R

K

A

S

V

S

I

I

Q

S

E

V

V

I

L

-

D

-

A

S

K

T

A

H

L

L

F

L

D

Q

L

L

L

I

L

K

T

T

L

L

A

V

E

E

K

R

H

A

Q

A

N

I

D

E

L

I

-

D

-

I

M

M

P

P

G

G

Y

Y

T

T

H

H

T

L

Q

Q

L

K

A

A

M

L

P

P

S

I

S

R

F

W

G

S

L

Q

W

F

F

L

G

L

A

S

M

H

A

A

E

V

S

A

L

L

A

T

E

R

D

D

M

S

E

E

L

R

W

L

L

G

A

E

A

V

Y

K

N

K

L

R

A

I

D

T

R

V

N

L

P

P

L

L

G

G

S

S

A

G

A

A

G

L

Y

A

G

G

S

N

S

P

F

L

P

E

L

I

N

D

R

R

T

E

M

L

T

L

T

R

R

S

L

E

L

L

G

D

F

M

K

T

D

S

M

I

H

T

Y

L

N

N

V

S

I

I

N

D

A

A

Q

I

N

S

N

E

R

R

G

D

K

F

T

V

E

V

K

E

A

L

I

I

A

S

F

V

A

A

M

T

A

L

G

L

M

M

A

I

G

H

T

-

L

L

N

S

R

K

L

L

S

A

A

E

D

D

I

L

I

I

F

F

M

S

N

T

Q

T

H

E

F

F

G

G

F

F

V

V

K

T

F

L

P

S

D

D

N

A

L

Y

T

S

T

T

G

G

S

S

I

L

M

L

P

P

H

Q

K

K

N

N

P

P

D

D

V

S

F

L

E

E

L

L

I

I

R

R

A

S

K

K

A

A

N

G

Q

R

I

V

Q

F

S

G

G

R

P

L

Q

A

N

A

L

I

M

L

M

M

Q

V

M

L

T

K

N

G

T

I

T

P

T

S

G

T

Y

F

H

S

R

K

D

D

L

L

Q

Q

L

E

L

D

K

K

E

E

T

A

T

V

F

L

P

D

D

V

F

V

E

D

K

T

L

L

K

T

D

A

C

V

L

L

Q

Q

I

V

T

A

K

T

F

G

M

V

L

I

E

S

H

T

I

L

E

Q

I

V

K

N

P

K

D

E

I

N

L

M

E

E

D

K

K

A

F

L

Y

T

K

P

H

E

L

L

F

L

S

S

V

T

E

D

V

L

V

A

N

L

D

Y

L

L

V

V

L

R

Q

K

G

G

M

M

P

P

F

I

R

R

D

Q

A

A

Y

Q

K

T

K

A

V

S

G

G

Sites not aligning to the query:

2 modified: Blocked amino end (Ala)

6ienC Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
34% identity, 84% coverage: 25:399/444 of query aligns to 14:388/418 of 6ienC

query
sites
6ienC

F

F

D

D

I

W

V

V

L

L

A

A

P

P

F

Y

D

D

V

L

L

T

G

A

S

S

L

R

A

A

H

H

A

T

T

M

M

V

L

L

E

F

S

R

I

A

D

G

L

L

T

T

K

E

E

E

E

Q

L

R

A

D

I

G

L

L

K

L

K

A

G

G

L

L

K

D

D

S

I

L

Y

A

Q

Q

E

D

I

V

Q

A

E

D

G

G

T

S

F

F

T

G

I

P

D

L

P

V

G

T

V

D

E

E

D

D

V

V

H

H

S

A

Q

A

V

L

E

E

F

R

L

G

L

L

T

I

E

D

R

R

Y

V

G

G

-

P

D

D

V

L

G

G

K

G

K

R

L

L

H

R

S

A

G

G

R

R

S
|
S

R
|
R

N
|
N

D

D

Q

Q

V
|
V

A

A

V

A

D

L

L

F

K

R

L

M

Y

W

Y

L

R

R

A

D

V

A

I

V

Q

R

E

R

V

V

L

-

D

-

A

A

K

T

A

G

L

V

F

L

D

D

L

V

L

G

T

A

L

L

A

A

E

E

K

Q

-

A

H

H

Q

P

N

S

D

A

L

I

M

M

P

P

G

G

Y

K

T
|
T

H
|
H

T

L

Q

Q

L

S

A

A

M

Q

P

P

S

I

S

L

F

L

G

A

L

H

W

H

F

L

G

L

A

A

M

H

A

A

E

H

S

P

L

L

A

L

E

R

D

D

M

L

E

D

L

R

W

I

L

V

A

D

A

F

Y

D

N

K

L

R

A

A

D

A

R

V

N

S

P

P

L

Y

G

G

S

S

A

G

A

A

G

L

Y

A

G

G

S

S

F

L

P

G

L

L

N

D

R

P

T

D

M

A

T

I

T

A

R

A

L

D

L

L

G

G

F

F

K

S

D

A

M

A

H

A

Y

D

N

N

V

S

I

V

N

D

A

A

Q

T

N

A

R

R

G

D

K

F

T

A

E

A

K

E

A

A

I

A

A

F

F

V

A

F

M

A

A

M

G

-

M

I

A

A

G

V

T

D

L

L

N

S

R

R

L

L

S

A

A

E

D

D

I

I

I

V

F

W

M

S

N

S

Q

T

H

E

F

F

G

G

F

Y

V

V

K

T

F

L

P

H

D

D

N

S

L

W

T

S

T

T

G

G

S
|
S

I

I

M
|
M

P

P

H

Q

K
|
K

K

K

N

N

P

P

D

D

V

I

F

A

E

E

L

L

I

A

R

R

A

G

K

K

A

S

N

G

Q

R

I

L

Q

I

S

G

G

N

P

L

Q

A

N

G

L

L

M

L

M

A

Q

T

M

L

T

K

N

A

T

Q

T

P

T

L

G

A

Y
|
Y

H

N

R

R

D

D

L

L

Q
|
Q

L

E

L

D

K
|
K

E

E

T

P

T

V

F

F

P

D

D

S

F

V

E

A

K

Q

L

L

K

E

D

L

C

L

L

L

Q

P

I

A

T

M

K

A

F

G

M

L

L

V

E

A

H

S

I

L

E

T

I

F

K

N

P

V

D

Q

-

R

I

M

L

A

E

E

D

L

K

A

F

P

Y

A

K

G

H

Y

L

T

F

L

S

A

V

T

E

D

V

L

V

A

N

E

D

W

L

L

V

V

L

R

Q

Q

G

G

M

V

P

P

F

F

R

R

D

S

A

A

Y

H

K

R

K

E

V

V

G

-

L

L

D

T

I

I

E

E

S

G

D

S

D

V

F

S

A

A

P

R

D

D

binding arginine: R98 (= R108), N99 (= N109), V102 (= V112), Y306 (= Y317), Q311 (= Q322), K314 (= K325)
binding argininosuccinate: T144 (= T154), H145 (= H155), S266 (= S277), S267 (= S278), M269 (= M280), K272 (= K283)
binding fumaric acid: S97 (= S107), R98 (= R108), N99 (= N109)

6ienB Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
34% identity, 81% coverage: 25:385/444 of query aligns to 14:375/454 of 6ienB

query
sites
6ienB

F

F

D

D

I

W

V

V

L

L

A

A

P

P

F

Y

D

D

V

L

L

T

G

A

S

S

L

R

A

A

H

H

A

T

T

M

M

V

L

L

E

F

S

R

I

A

D

G

L

L

T

T

K

E

E

E

E

Q

L

R

A

D

I

G

L

L

K

L

K

A

G

G

L

L

K

D

D

S

I

L

Y

A

Q

Q

E

D

I

V

Q

A

E

D

G

G

T

S

F

F

T

G

I

P

D

L

P

V

G

T

V

D

E

E

D

D

V

V

H

H

S

A

Q

A

V

L

E

E

F

R

L

G

L

L

T

I

E

D

R

R

Y

V

G

G

-

P

D

D

V

L

G

G

K

G

K

R

L

L

H

R

S

A

G

G

R

R

S
|
S

R
|
R

N
|
N

D

D

Q

Q

V

V

A

A

V

A

D

L

L

F

K

R

L

M

Y

W

Y

L

R

R

A

D

V

A

I

V

Q

R

E

R

V

V

L

-

D

-

A

A

K

T

A

G

L

V

F

L

D

D

L

V

L

G

T

A

L

L

A

A

E

E

K

Q

-

A

H

H

Q

P

N

S

D

A

L

I

M

M

P

P

G

G

Y

K

T
|
T

H
|
H

T

L

Q

Q

L

S

A

A

M

Q

P

P

S

I

S

L

F

L

G

A

L

H

W

H

F

L

G

L

A

A

M

H

A

A

E

H

S

P

L

L

A

L

E

R

D

D

M

L

E

D

L

R

W

I

L

V

A

D

A

F

Y

D

N

K

L

R

A

A

D

A

R

V

N

S

P

P

L

Y

G

G

S

S

A

G

A

A

G

L

Y

A

G

G

S

S

F

L

P

G

L

L

N

D

R

P

T

D

M

A

T

I

T

A

R

A

L

D

L

L

G

G

F

F

K

S

D

A

M

A

H

A

Y

D

N

N

V

S

I

V

N

D

A

A

Q

T

N

A

R

R

G

D

K

F

T

A

E

A

K

E

A

A

I

A

A

F

F

V

A

F

M

A

A

M

G

-

M

I

A

A

G

V

T

D

L

L

N

S

R

R

L

L

S

A

A

E

D

D

I

I

I

V

F

W

M

S

N

S

Q

T

H

E

F

F

G

G

F

Y

V

V

K

T

F

L

P

H

D

D

N

S

L

W

T

S

T

T

G

G

S
|
S

I

I

M
|
M

P

P

H

Q

K
|
K

K

K

N

N

P

P

D

D

V

I

F

A

E

E

L

L

I

A

R

R

A

G

K

K

A

S

N

G

Q

R

I

L

Q

I

S

G

G

N

P

L

Q

A

N

G

L

L

M

L

M

A

Q

T

M

L

T

K

N

A

T

Q

T

P

T

L

G

A

Y
|
Y

H

N

R

R

D

D

L

L

Q
|
Q

L

E

L

D

K
|
K

E

E

T

P

T

V

F

F

P

D

D

S

F

V

E

A

K

Q

L

L

K

E

D

L

C

L

L

L

Q

P

I

A

T

M

K

A

F

G

M

L

L

V

E

A

H

S

I

L

E

T

I

F

K

N

P

V

D

Q

-

R

I

M

L

A

E

E

D

L

K

A

F

P

Y

A

K

G

H

Y

L

T

F

L

S

A

V

T

E

D

V

L

V

A

N

E

D

W

L

L

V

V

L

R

Q

Q

G

G

M

V

P

P

F

F

R

R

D

S

A

A

Y

H

K

E

binding argininosuccinate: S97 (= S107), R98 (= R108), N99 (= N109), T144 (= T154), H145 (= H155), S266 (= S277), S267 (= S278), M269 (= M280), K272 (= K283), Y306 (= Y317), Q311 (= Q322), K314 (= K325)

6ienA Substrate/product bound argininosuccinate lyase from mycobacterium tuberculosis (see paper)
33% identity, 81% coverage: 25:385/444 of query aligns to 14:373/452 of 6ienA

query
sites
6ienA

F

F

D

D

I

W

V

V

L

L

A

A

P

P

F

Y

D

D

V

L

L

T

G

A

S

S

L

R

A

A

H

H

A

T

T

M

M

V

L

L

E

F

S

R

I

A

D

G

L

L

T

T

K

E

E

E

E

Q

L

R

A

D

I

G

L

L

K

L

K

A

G

G

L

L

K

D

D

S

I

L

Y

A

Q

Q

E

D

I

V

Q

A

E

D

G

G

T

S

F

F

T

G

I

P

D

L

P

V

G

T

V

D

E

E

D

D

V

V

H

H

S

A

Q

A

V

L

E

E

F

R

L

G

L

L

T

I

E

D

R

R

Y

V

G

G

-

P

D

D

V

L

G

G

K

G

K

R

L

L

H

R

S

A

G

G

R

R

S

S

R
|
R

N
|
N

D

D

Q

Q

V
|
V

A

A

V

A

D

L

L

F

K

R

L

M

Y

W

Y

L

R

R

A

D

V

A

I

V

Q

R

E

R

V

V

L

-

D

-

A

A

K

T

A

G

L

V

F

L

D

D

L

V

L

G

T

A

L

L

A

A

E

E

K

Q

-

A

H

H

Q

P

N

S

D

A

L

I

M

M

P

P

G

G

Y

K

T
|
T

H
|
H

T

L

Q

Q

L

S

A

A

M

Q

P

P

S

I

S

L

F

L

G

A

L

H

W

H

F

L

G

L

A

A

M

H

A

A

E

H

S

P

L

L

A

L

E

R

D

D

M

L

E

D

L

R

W

I

L

V

A

D

A

F

Y

D

N

K

L

R

A

A

D

A

R

V

N

S

P

P

L

Y

G

G

S

S

A

G

A

A

G

L

Y

A

G

G

S

S

F

L

P

G

L

L

N

D

R

P

T

D

M

A

T

I

T

A

R

A

L

D

L

L

G

G

F

F

K

S

D

A

M

A

H

A

Y

D

N

N

V

S

I

V

N

D

A

A

Q

T

N

A

R

R

G

D

K

F

T

A

E

A

K

E

A

A

I

A

A

F

F

V

A

F

M

A

A

M

G

-

M

I

A

A

G

V

T

D

L

L

N

S

R

R

L

L

S

A

A

E

D

D

I

I

I

V

F

W

M

S

N

S

Q

T

H

E

F

F

G

G

F

Y

V

V

K

T

F

L

P

H

D

D

N

S

L

W

T

S

T

T

G

G

S
|
S

I

-

M

-

P

P

H

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

I

F

A

E

E

L

L

I

A

R

R

A

G

K

K

A

S

N

G

Q

R

I

L

Q

I

S

G

G

N

P

L

Q

A

N

G

L

L

M

L

M

A

Q

T

M

L

T

K

N

A

T

Q

T

P

T

L

G

A

Y
|
Y

H

N

R

R

D

D

L

L

Q
|
Q

L

E

L

D

K
|
K

E

E

T

P

T

V

F

F

P

D

D

S

F

V

E

A

K

Q

L

L

K

E

D

L

C

L

L

L

Q

P

I

A

T

M

K

A

F

G

M

L

L

V

E

A

H

S

I

L

E

T

I

F

K

N

P

V

D

Q

-

R

I

M

L

A

E

E

D

L

K

A

F

P

Y

A

K

G

H

Y

L

T

F

L

S

A

V

T

E

D

V

L

V

A

N

E

D

W

L

L

V

V

L

R

Q

Q

G

G

M

V

P

P

F

F

R

R

D

S

A

A

Y

H

K

E

binding argininosuccinate: R98 (= R108), N99 (= N109), V102 (= V112), T144 (= T154), H145 (= H155), Y304 (= Y317), Q309 (= Q322), K312 (= K325)
binding fumaric acid: S266 (= S277), S267 (= S278), K270 (= K283), N272 (= N285)

6g3hA Crystal structure of edds lyase in complex with ss-edds (see paper)
29% identity, 61% coverage: 31:303/444 of query aligns to 37:301/497 of 6g3hA

query
sites
6g3hA

P

P

F

Y

D

D

V

S

L

Q

G

I

S

H

L

R

A

A

H

H

A

V

T

V

M

M

L

L

E

T

S

E

I

Q

D

G

L

I

L

L

T

T

K

V

E

E

L

S

A

A

I

T

L

I

K

L

K

S

G

G

L

L

K

A

D

Q

I

V

Y

D

Q

E

E

-

I

-

Q

-

E

-

G

-

T

-

F

L

T

A

I

A

D

T

P

D

G

G

V

S

E

L

D

R

V

T

H

Y

S

L

Q

P

V

Y

E

E

F

A

L

A

L

L

T

K

E

R

R

T

Y

I

G

G

D

S

V

V

G

A

K

G

K

K

L

M

H

H

S

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

V

A

D

G

L

K

K

R

L

M

Y

F

Y

L

R

R

A

D

V

Q

I

L

Q

L

E

R

V

T

L

I

D

E

D

A

A

V

K

I

A

G

L

Y

F

R

D

E

L

A

L

V

T

H

L

K

A

A

E

A

K

D

H

H

Q

L

N

D

D

T

L

V

M

M

P

V

G

V

Y

Y

T

T

H

Q

T

R

Q

K

L

E

A

A

M

Q

P

P

S

I

S

T

F

L

G

G

L

H

W

Y

F

L

G

M

A

A

M

I

A

S

E

E

S

N

L

L

A

A

E

K

D

N

M

L

E

D

L

R

W

Y

L

R

A

E

A

L

Y

Y

N

A

L

R

A

I

D

N

R

L

N

C

P

P

L

L

G

G

S

A

A

A

G

T

Y

A

G

G

S

T

S

G

F

W

P

P

L

L

N

N

R

R

T

D

M

R

T

T

T

S

R

A

L

L

G

G

F

F

K

D

D

G

M

L

H

V

Y

V

N

N

V

S

I

I

N

E

A

G

Q

V

N

A

N

G

R

W

G

D

K

H

T

V

E

A

K

E

A

H

I

-

A

A

F

F

A

V

M

N

A

A

G

V

M

F

A

L

G

S

T

G

L

L

N

S

R

R

L

L

S

A

A

S

D

E

I

I

I

Q

I

L

F

W

M

S

N

T

Q

D

H

E

F

Y

G

Q

F

V

V

A

K

E

F

L

P

D

D

A

N

S

L

F

T

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

H

Q

K
|
K

K

K

N
|
N

P

P

D
|
D

V

S

F

L

E

E

L
x
R

I

S

R

R

A

-

K

K

A

A

N

A

Q

F

I

A

Q

A

S

M

G

G

P

P

binding (2~{S})-2-[2-[[(2~{S})-1,4-bis(oxidanyl)-1,4-bis(oxidanylidene)butan-2-yl]amino]ethylamino]butanedioic acid: S276 (= S277), S277 (= S278), M279 (= M280), K282 (= K283), N284 (= N285), D286 (= D287), R290 (≠ L291)

Sites not aligning to the query:

binding (2~{S})-2-[2-[[(2~{S})-1,4-bis(oxidanyl)-1,4-bis(oxidanylidene)butan-2-yl]amino]ethylamino]butanedioic acid: 22

6g3gA Crystal structure of edds lyase in complex with succinate (see paper)
29% identity, 61% coverage: 31:303/444 of query aligns to 37:301/497 of 6g3gA

query
sites
6g3gA

P

P

F

Y

D

D

V

S

L

Q

G

I

S

H

L

R

A

A

H

H

A

V

T

V

M

M

L

L

E

T

S

E

I

Q

D

G

L

I

L

L

T

T

K

V

E

E

L

S

A

A

I

T

L

I

K

L

K

S

G

G

L

L

K

A

D

Q

I

V

Y

D

Q

E

E

-

I

-

Q

-

E

-

G

-

T

-

F

L

T

A

I

A

D

T

P

D

G

G

V

S

E

L

D

R

V

T

H

Y

S

L

Q

P

V

Y

E

E

F

A

L

A

L

L

T

K

E

R

R

T

Y

I

G

G

D

S

V

V

G

A

K

G

K

K

L

M

H

H

S

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

V

A

D

G

L

K

K

R

L

M

Y

F

Y

L

R

R

A

D

V

Q

I

L

Q

L

E

R

V

T

L

I

D

E

D

A

A

V

K

I

A

G

L

Y

F

R

D

E

L

A

L

V

T

H

L

K

A

A

E

A

K

D

H

H

Q

L

N

D

D

T

L

V

M

M

P

V

G

V

Y

Y

T

T

H

Q

T

R

Q

K

L

E

A

A

M

Q

P

P

S

I

S

T

F

L

G

G

L

H

W

Y

F

L

G

M

A

A

M

I

A

S

E

E

S

N

L

L

A

A

E

K

D

N

M

L

E

D

L

R

W

Y

L

R

A

E

A

L

Y

Y

N

A

L

R

A

I

D

N

R

L

N

C

P

P

L

L

G

G

S

A

A

A

G

T

Y

A

G

G

S

T

S

G

F

W

P

P

L

L

N

N

R

R

T

D

M

R

T

T

T

S

R

A

L

L

G

G

F

F

K

D

D

G

M

L

H

V

Y

V

N

N

V

S

I

I

N

E

A

G

Q

V

N

A

N

G

R

W

G

D

K

H

T

V

E

A

K

E

A

H

I

-

A

A

F

F

A

V

M

N

A

A

G

V

M

F

A

L

G

S

T

G

L

L

N

S

R

R

L

L

S

A

A

S

D

E

I

I

I

Q

I

L

F

W

M

S

N

T

Q

D

H

E

F

Y

G

Q

F

V

V

A

K

E

F

L

P

D

D

A

N

S

L

F

T

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

H

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

S

F

L

E

E

L

R

I

S

R

R

A

-

K

K

A

A

N

A

Q

F

I

A

Q

A

S

M

G

G

P

P

binding succinic acid: S276 (= S277), S277 (= S278), M279 (= M280), K282 (= K283), N284 (= N285)

6g3fA Crystal structure of edds lyase in complex with fumarate (see paper)
29% identity, 61% coverage: 31:303/444 of query aligns to 37:301/497 of 6g3fA

query
sites
6g3fA

P

P

F

Y

D

D

V

S

L

Q

G

I

S

H

L

R

A

A

H

H

A

V

T

V

M

M

L

L

E

T

S

E

I

Q

D

G

L

I

L

L

T

T

K

V

E

E

L

S

A

A

I

T

L

I

K

L

K

S

G

G

L

L

K

A

D

Q

I

V

Y

D

Q

E

E

-

I

-

Q

-

E

-

G

-

T

-

F

L

T

A

I

A

D

T

P

D

G

G

V

S

E

L

D

R

V

T

H

Y

S

L

Q

P

V

Y

E

E

F

A

L

A

L

L

T

K

E

R

R

T

Y

I

G

G

D

S

V

V

G

A

K

G

K

K

L

M

H

H

S

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

V

A

D

G

L

K

K

R

L

M

Y

F

Y

L

R

R

A

D

V

Q

I

L

Q

L

E

R

V

T

L

I

D

E

D

A

A

V

K

I

A

G

L

Y

F

R

D

E

L

A

L

V

T

H

L

K

A

A

E

A

K

D

H

H

Q

L

N

D

D

T

L

V

M

M

P

V

G

V

Y

Y

T

T

H

Q

T

R

Q

K

L

E

A

A

M

Q

P

P

S

I

S

T

F

L

G

G

L

H

W

Y

F

L

G

M

A

A

M

I

A

S

E

E

S

N

L

L

A

A

E

K

D

N

M

L

E

D

L

R

W

Y

L

R

A

E

A

L

Y

Y

N

A

L

R

A

I

D

N

R

L

N

C

P

P

L

L

G

G

S

A

A

A

G

T

Y

A

G

G

S

T

S

G

F

W

P

P

L

L

N

N

R

R

T

D

M

R

T

T

T

S

R

A

L

L

G

G

F

F

K

D

D

G

M

L

H

V

Y

V

N

N

V

S

I

I

N

E

A

G

Q

V

N

A

N

G

R

W

G

D

K

H

T

V

E

A

K

E

A

H

I

-

A

A

F

F

A

V

M

N

A

A

G

V

M

F

A

L

G

S

T

G

L

L

N

S

R

R

L

L

S

A

A

S

D

E

I

I

I

Q

I

L

F

W

M

S

N

T

Q

D

H

E

F

Y

G

Q

F

V

V

A

K

E

F

L

P

D

D

A

N

S

L

F

T

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

H

Q

K
|
K

K

K

N
|
N

P

P

D

D

V

S

F

L

E

E

L

R

I

S

R

R

A

-

K

K

A

A

N

A

Q

F

I

A

Q

A

S

M

G

G

P

P

binding fumaric acid: S276 (= S277), S277 (= S278), M279 (= M280), K282 (= K283), N284 (= N285)

6g3iA Crystal structure of edds lyase in complex with n-(2-aminoethyl) aspartic acid (aeaa) (see paper)
29% identity, 61% coverage: 31:303/444 of query aligns to 37:301/496 of 6g3iA

query
sites
6g3iA

P

P

F

Y

D

D

V

S

L

Q

G

I

S

H

L

R

A

A

H

H

A

V

T

V

M

M

L

L

E

T

S

E

I

Q

D

G

L

I

L

L

T

T

K

V

E

E

L

S

A

A

I

T

L

I

K

L

K

S

G

G

L

L

K

A

D

Q

I

V

Y

D

Q

E

E

-

I

-

Q

-

E

-

G

-

T

-

F

L

T

A

I

A

D

T

P

D

G

G

V

S

E

L

D

R

V

T

H

Y

S

L

Q

P

V

Y

E

E

F

A

L

A

L

L

T

K

E

R

R

T

Y

I

G

G

D

S

V

V

G

A

K

G

K

K

L

M

H

H

S

I

G

G

R

R

S

S

R

R

N

N

D

D

Q

L

V

A

A

N

V

A

D

G

L

K

K

R

L

M

Y

F

Y

L

R

R

A

D

V

Q

I

L

Q

L

E

R

V

T

L

I

D

E

D

A

A

V

K

I

A

G

L

Y

F

R

D

E

L

A

L

V

T

H

L

K

A

A

E

A

K

D

H

H

Q

L

N

D

D

T

L

V

M

M

P

V

G

V

Y

Y

T

T

H

Q

T

R

Q

K

L

E

A

A

M

Q

P

P

S

I

S

T

F

L

G

G

L

H

W

Y

F

L

G

M

A

A

M

I

A

S

E

E

S

N

L

L

A

A

E

K

D

N

M

L

E

D

L

R

W

Y

L

R

A

E

A

L

Y

Y

N

A

L

R

A

I

D

N

R

L

N

C

P

P

L

L

G

G

S

A

A

A

G

T

Y

A

G

G

S

T

S

G

F

W

P

P

L

L

N

N

R

R

T

D

M

R

T

T

T

S

R

A

L

L

G

G

F

F

K

D

D

G

M

L

H

V

Y

V

N

N

V

S

I

I

N

E

A

G

Q

V

N

A

N

G

R

W

G

D

K

H

T

V

E

A

K

E

A

H

I

-

A

A

F

F

A

V

M

N

A

A

G

V

M

F

A

L

G

S

T

G

L

L

N

S

R

R

L

L

S

A

A

S

D

E

I

I

I

Q

I

L

F

W

M

S

N

T

Q

D

H

E

F

Y

G

Q

F

V

V

A

K

E

F

L

P

D

D

A

N

S

L

F

T

A

T

G

G

T

S
|
S

I

I

M
|
M

P

P

H

Q

K
|
K

K

K

N
|
N

P

P

D
|
D

V

S

F

L

E

E

L
x
R

I

S

R

R

A

-

K

K

A

A

N

A

Q

F

I

A

Q

A

S

M

G

G

P

P

binding (2~{S})-2-(2-azanylethylamino)butanedioic acid: S276 (= S277), S277 (= S278), M279 (= M280), K282 (= K283), N284 (= N285), D286 (= D287)
binding fumaric acid: R290 (≠ L291)

Sites not aligning to the query:

binding fumaric acid: 22

P12047 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor; EC 4.3.2.2 from Bacillus subtilis (strain 168) (see paper)
24% identity, 54% coverage: 84:321/444 of query aligns to 68:303/431 of P12047

query
sites
P12047

H

H

S

D

Q

V

V

V

E

A

F

F

L

T

-

R

-

A

L

V

T

S

E

E

R

S

Y

L

G

G

D

E

V

E

G

R

K

K

K

W

L

V

H
|
H

S

Y

G

G

R

L

S

T

R

S

N

T

D

D

Q

V

V

V

A

D

V

T

D

A

L

L

K

S

L

Y

Y

L

R

K

A

Q

V

A

I

N

Q

D

E

I

V

L

L

L

D

K

D

D

A

L

K

E

A

R

L

F

F

V

D

D

L

I

L

K

T

E

L

K

A

A

E

K

K

E

H

H

Q

K

N

Y

D

T

L

V

M

M

P

M

G

G

Y

R

T

T

H
|
H

T

G

Q

V

L

H

A

A

M

E

P

P

S

T

F

F

G

G

L

L

W

K

F

L

G

A

A

L

M

W

A

H

E

E

S

E

L

M

A

K

E

R

D

N

M

L

E

E

L

R

W

F

L

K

A

Q

A

A

Y

K

N

A

L

G

A

I

D

E

R

V

N

G

P

K

L

I

G

S

S

G

A

A

A

V

G

G

-

T

Y

Y

G

A

S

N

S

I

F

D

P

P

L

F

N

V

R

E

T

Q

M

Y

T

V

T

C

R

E

L

K

L

L

G

G

F

L

K

K

D

A

M

A

H

P

Y

I

N

S

V

T

I
x
Q

N

T

A

L

Q

Q

N

R

N

D

R

R

G

H

K

A

T

D

E

Y

K

M

A

A

I

-

A

-

F

-

A

T

M

L

A

A

G

L

M

I

A

A

G

T

T

S

L

I

N

E

R

K

L

F

S

A

A

V

D

E

I

I

I

R

I

G

F

L

M

Q

N

K

Q

S

H

E

F

T

G

R

F

E

V

V

-

E

K

E

F

F

P

F

D

A

N

K

L

G

T

Q

T

K

G

G

S

S

I

A

M

M

P

P

H

H

K

K

K

R

N
|
N

P

P

D

I

V

G

F

S

E

E

L

N

I

M

R

T

A

G

K

M

A

A

N

R

Q

V

I

I

Q

R

S

G

G

-

P

-

Q

-

N

Y

L

M

M

M

M

T

Q

A

M

Y

T

E

N

N

T

V

T

P

T

L

G

W

Y

H

H

E

R
|
R

D

D

L

I

H89 (= H103) mutation to Q: Abolishes enzyme activity.
H141 (= H155) mutation to Q: Abolishes enzyme activity.
Q212 (≠ I225) mutation to E: Decreases catalytic activity 1000-fold.; mutation to M: Abolishes enzyme activity.
N270 (= N285) mutation N->D,L: Abolishes enzyme activity.
R301 (= R319) mutation R->K,Q: Abolishes enzyme activity.

A0A0K2JL82 Nitrosuccinate lyase; EC 4.3.99.5 from Streptomyces cremeus (see paper)
26% identity, 45% coverage: 145:342/444 of query aligns to 160:360/476 of A0A0K2JL82

query
sites
A0A0K2JL82

H

H

Q

R

N

D

D

T

L

P

M

M

P

A

G

G

Y

R

T

T

H

L

T

A

Q

L

L

H

A

A

M

V

P

P

S

T

F

F

G

G

L

L

-

K

-

A

-

G

W

W

F

L

G

E

A

L

M

V

A

S

E

E

S

A

L

A

A

G

E

R

D

-

M

-

E

-

L

-

W

-

L

-

A

-

A

V

Y

A

N

R

L

L

A
x
R

D

D

R

G

N

L

P

P

-

F

-

S

L

L

G

G

S

G

A

A

G

G

Y

T

G

L

S

A

S

G

F

Y

P

F

L

G

N

D

R

R

T

T

M

D

T

R

T

G

R

D

L

P

L

A

G

V

F

L

K

L

D

D

M

R

H

L

Y

L

N

D

V

A

I

Y

N

A

A

A

Q

E

N

T

N

G

R

L

G

A

-

R

-

P

-

V

-

L

-

P

-

W

-
x
H

-

V

-

L

K

R

T

T

E

P

K

V

A

A

-

D

I

L

A

A

F

A

A

V

M

L

A

A

G

F

M

T

A

A

G

G

T

A

L

L

N

G

R

K

L

I

S

A

A

V

D

D

I

V

I

Q

I

S

F

L

M

A

N

R

Q

T

H

E

F

V

G

A

F

E

V

V

K

A

F

E

P

P

D

A

N

V

L

E

T

G

T

R

G

G

-

A

S
|
S

S

S

I

A

M

M

P

P

H

H

K
|
K

K

R

N
|
N

P

P

D

V

V

L

F

S

E

T

L

L

I

I

R

R

A

S

K

A

A

A

N

L

Q

Q

I

V

Q

P

S

A

G

L

P

A

-

T

-

G

-

L

-

T

Q

Q

N

C

L

L

M

V

M

S

Q

E

M

D

T

E

N
x
R

T

S

T

A

T

G

G

A

Y

W

H

H

R

A

D

E

L

W

Q

Q

L

P

L

L

K

R

E

E

T

-

F

-

P

-

D

-

F

-

E

-

K

-

L

-

K

-

D

-

C

C

L

L

Q

R

I

L

T

T

R201 (≠ A189) binding fumarate
H253 (vs. gap) mutation to A: Loss of activity.
S302 (= S277) mutation to A: Loss of activity.
K308 (= K283) binding fumarate; mutation to A: Loss of activity.
N310 (= N285) binding fumarate; mutation to A: Loss of activity.
R341 (≠ N312) mutation to A: Loss of activity.

Sites not aligning to the query:

93 N→A: Slight decrease in activity.
125 mutation D->N,V: Almost loss of activity.
137 binding fumarate
140 binding fumarate

5hw2A Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with fumaric acid
37% identity, 18% coverage: 96:173/444 of query aligns to 82:160/419 of 5hw2A

query
sites
5hw2A

G

A

D

E

V

T

G

G

K

K

K

F

L

F

H
|
H

S

F

G

G

R

V

S
x
T

R
x
S

N

S

D

D

Q

I

V

I

A

D

V

S

D

A

L

L

K

S

L

L

Y

Q

Y

I

R

R

A

D

V

S

I

M

Q

S

E

Y

V

V

L

I

D

K

D

D

A

L

K

E

A

A

L

L

F

C

D

D

L

S

L

L

T

T

L

K

A

A

E

E

K

E

H

T

Q

K

N

E

D

I

L

I

M

T

P

M

G

G

Y

R

T
x
S

H
|
H

T

G

Q

M

L

F

A

A

M

E

P

P

S

M

S

S

F

F

G

G

L

Q

W

K

F

F

-

L

G

G

A

A

M

Y

A

V

E

E

active site: S140 (≠ T154), H141 (= H155)
binding fumaric acid: H89 (= H103), T93 (≠ S107), S94 (≠ R108)

Sites not aligning to the query:

active site: 67, 256, 263
binding fumaric acid: 67

4eeiB Crystal structure of adenylosuccinate lyase from francisella tularensis complexed with amp and succinate
37% identity, 18% coverage: 96:173/444 of query aligns to 82:160/423 of 4eeiB

query
sites
4eeiB

G

A

D

E

V

T

G

G

K

K

K

F

L

F

H

H

S

F

G

G

R

V

S

T

R

S

N

S

D

D

Q

I

V

I

A

D

V

S

D

A

L

L

K

S

L

L

Y

Q

Y

I

R

R

A

D

V

S

I

M

Q

S

E

Y

V

V

L

I

D

K

D

D

A

L

K

E

A

A

L

L

F

C

D

D

L

S

L

L

T

T

L

K

A

A

E

E

K

E

H

T

Q

K

N

E

D

I

L

I

M

T

P

M

G

G

Y

R

T
x
S

H
|
H

T

G

Q

M

L

F

A

A

M

E

P

P

S

M

S

S

F

F

G

G

L

Q

W

K

F

F

-

L

G

G

A

A

M

Y

A

V

E

E

active site: S140 (≠ T154), H141 (= H155)

Sites not aligning to the query:

active site: 67, 256, 263
binding adenosine monophosphate: 66, 67, 68, 212, 289, 291, 294, 298

Q9X0I0 Adenylosuccinate lyase; ASL; Adenylosuccinase; ASase; EC 4.3.2.2 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
21% identity, 75% coverage: 53:384/444 of query aligns to 35:372/431 of Q9X0I0

query
sites
Q9X0I0

E

E

L

L

A

G

I

M

L

I

K

P

K

K

G

G

L

V

K

T

D

E

-

R

-

I

-

R

-

N

-

A

-

K

-

I

-

D

-

V

-

E

I

L

Y

F

Q

K

E

K

I

I

Q

E

E

E

G

K

T

T

-

N

F

H

T

D

I

V

D

V

P

A

G

F

V

V

E

E

D

G

V

I

H

G

S

S

Q

M

V

I

E

-

F

-

L

-

T

-

E

-

R

-

Y

-

G

G

D

E

V

D

G

S

K

R

K

F

L

F

H

H

S

Y

G

G

R

L

S

T

R

S

N

S

D

D

Q

V

V

L

A

D

V

T

D

A

L

N

K

S

L

L

Y

A

Y

L

R

V

A

E

V

A

I

G

Q

K

E

I

V

L

L

L

D

E

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H141 (= H155) active site, Proton donor/acceptor

Query Sequence

>Echvi_3852 FitnessBrowser__Cola:Echvi_3852
MKLWQKNTTSTKEVEQFTIGRDPEFDIVLAPFDVLGSLAHATMLESIDLLTKEELAILKK
GLKDIYQEIQEGTFTIDPGVEDVHSQVEFLLTERYGDVGKKLHSGRSRNDQVAVDLKLYY
RAVIQEVLDDAKALFDLLLTLAEKHQNDLMPGYTHTQLAMPSSFGLWFGAMAESLAEDME
LWLAAYNLADRNPLGSAAGYGSSFPLNRTMTTRLLGFKDMHYNVINAQNNRGKTEKAIAF
AMAGMAGTLNRLSADIIIFMNQHFGFVKFPDNLTTGSSIMPHKKNPDVFELIRAKANQIQ
SGPQNLMMQMTNTTTGYHRDLQLLKETTFPDFEKLKDCLQITKFMLEHIEIKPDILEDKF
YKHLFSVEVVNDLVLQGMPFRDAYKKVGLDIESDDFAPDHTVNHSHEGSIGQLCLDEIRT
KMETALAKFDFSAIETSYQKLLEA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory