SitesBLAST
Comparing GFF1006 FitnessBrowser__Phaeo:GFF1006 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4amvA E.Coli glucosamine-6p synthase in complex with fructose-6p (see paper)
48% identity, 100% coverage: 2:602/602 of query aligns to 1:608/608 of 4amvA
- active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W74), N98 (= N98), G99 (= G99), Y248 (≠ H248), E481 (= E474), K485 (= K478), E488 (= E481), H504 (= H497), K603 (= K597)
- binding fructose -6-phosphate: G301 (= G297), T302 (= T298), S303 (≠ A299), S347 (= S343), Q348 (= Q344), S349 (= S345), T352 (= T348), S401 (= S396), K485 (= K478), E488 (= E481)
1jxaA Glucosamine 6-phosphate synthase with glucose 6-phosphate (see paper)
48% identity, 100% coverage: 2:602/602 of query aligns to 1:608/608 of 1jxaA
- active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W74), N98 (= N98), G99 (= G99), Y248 (≠ H248), E481 (= E474), K485 (= K478), E488 (= E481), H504 (= H497), K603 (= K597)
- binding glucose-6-phosphate: T302 (= T298), S303 (≠ A299), S347 (= S343), Q348 (= Q344), S349 (= S345), T352 (= T348), S401 (= S396), K485 (= K478), E488 (= E481)
2j6hA E. Coli glucosamine-6-p synthase in complex with glucose-6p and 5-oxo- l-norleucine (see paper)
48% identity, 100% coverage: 2:602/602 of query aligns to 1:608/608 of 2j6hA
- active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W74), N98 (= N98), G99 (= G99), Y248 (≠ H248), E481 (= E474), K485 (= K478), E488 (= E481), H504 (= H497), K603 (= K597)
- binding glucose-6-phosphate: T302 (= T298), S347 (= S343), Q348 (= Q344), S349 (= S345), T352 (= T348), V399 (= V394), S401 (= S396), E488 (= E481)
- binding 5-oxo-l-norleucine: C1 (= C2), R73 (= R73), W74 (= W74), T76 (= T76), H86 (= H86), N98 (= N98), G99 (= G99), D123 (= D123)
6svmA Crystal structure of human gfat-1 in complex with glucose-6-phosphate, l-glu, and udp-galnac (see paper)
36% identity, 100% coverage: 2:602/602 of query aligns to 1:660/660 of 6svmA
- active site: L7 (= L8), R32 (= R27), W95 (= W74), N122 (= N98), G123 (= G99), E533 (= E474), K537 (= K478), E540 (= E481), H556 (= H497), K655 (= K597)
- binding glucose-6-phosphate: C353 (= C296), T355 (= T298), S356 (≠ A299), S400 (= S343), Q401 (= Q344), S402 (= S345), T405 (= T348), S453 (= S396), K537 (= K478), E540 (= E481)
- binding glutamic acid: C1 (= C2), R94 (= R73), W95 (= W74), T97 (= T76), H107 (= H86), G123 (= G99), D147 (= D123)
- binding magnesium ion: S434 (≠ A377), R435 (= R378), T437 (≠ S380)
- binding uridine-diphosphate-n-acetylgalactosamine: Q289 (= Q237), R322 (≠ Y270), G334 (≠ A281), G424 (= G367), T426 (≠ V369), S434 (≠ A377), T437 (≠ S380), C439 (≠ V382), G440 (≠ I383), V441 (≠ L384), H442 (≠ P385)
6r4eA Crystal structure of human gfat-1 in complex with glucose-6-phosphate and l-glu (see paper)
36% identity, 100% coverage: 2:602/602 of query aligns to 1:663/663 of 6r4eA
- active site: L7 (= L8), R32 (= R27), W95 (= W74), N122 (= N98), G123 (= G99), E536 (= E474), K540 (= K478), E543 (= E481), H559 (= H497), K658 (= K597)
- binding glucose-6-phosphate: T358 (= T298), S359 (≠ A299), S403 (= S343), Q404 (= Q344), S405 (= S345), T408 (= T348), S456 (= S396), K540 (= K478), E543 (= E481)
- binding glutamic acid: C1 (= C2), R94 (= R73), W95 (= W74), T97 (= T76), G123 (= G99), D147 (= D123)
6r4gA Crystal structure of human gfat-1 in complex with udp-glcnac (see paper)
36% identity, 99% coverage: 2:595/602 of query aligns to 1:652/652 of 6r4gA
- active site: L7 (= L8), R32 (= R27), W95 (= W74), N122 (= N98), G123 (= G99), E532 (= E474), K536 (= K478), E539 (= E481), H555 (= H497)
- binding glucose-6-phosphate: G353 (= G297), T354 (= T298), S355 (≠ A299), S399 (= S343), Q400 (= Q344), S401 (= S345), T404 (= T348), S452 (= S396), E539 (= E481)
- binding magnesium ion: S433 (≠ A377), R434 (= R378), T436 (≠ S380)
- binding uridine-diphosphate-n-acetylglucosamine: Q288 (= Q237), R321 (≠ Y270), G333 (≠ A281), G423 (= G367), T425 (≠ V369), S433 (≠ A377), T436 (≠ S380), C438 (≠ V382), G439 (≠ I383), V440 (≠ L384), H441 (≠ P385)
1mosA Isomerase domain of glucosamine 6-phosphate synthase complexed with 2- amino-2-deoxyglucitol 6-phosphate (see paper)
49% identity, 60% coverage: 244:602/602 of query aligns to 3:367/367 of 1mosA
- active site: E240 (= E474), K244 (= K478), E247 (= E481), H263 (= H497), K362 (= K597)
- binding 2-deoxy-2-amino glucitol-6-phosphate: T61 (= T298), S62 (≠ A299), S106 (= S343), Q107 (= Q344), S108 (= S345), T111 (= T348), K244 (= K478), E247 (= E481)
1morA Isomerase domain of glucosamine 6-phosphate synthase complexed with glucose 6-phosphate (see paper)
49% identity, 60% coverage: 244:602/602 of query aligns to 2:366/366 of 1morA
- active site: E239 (= E474), K243 (= K478), E246 (= E481), H262 (= H497), K361 (= K597)
- binding 6-O-phosphono-alpha-D-glucopyranose: T60 (= T298), S105 (= S343), Q106 (= Q344), S107 (= S345), T110 (= T348), V157 (= V394), A360 (= A596), K361 (= K597)
1moqA Isomerase domain of glucosamine 6-phosphate synthase complexed with glucosamine 6-phosphate (see paper)
49% identity, 60% coverage: 244:602/602 of query aligns to 2:366/366 of 1moqA
- active site: E239 (= E474), K243 (= K478), E246 (= E481), H262 (= H497), K361 (= K597)
- binding 2-amino-2-deoxy-6-O-phosphono-alpha-D-glucopyranose: T60 (= T298), S61 (≠ A299), S105 (= S343), Q106 (= Q344), S107 (= S345), T110 (= T348), V157 (= V394), A360 (= A596), K361 (= K597)
7dnrA Crystal structure of zn-bound sis domain of glucosamine-6-p synthase from e. Coli
48% identity, 58% coverage: 244:593/602 of query aligns to 2:357/357 of 7dnrA
P14742 Glutamine--fructose-6-phosphate aminotransferase [isomerizing]; GFAT; D-fructose-6-phosphate amidotransferase; Hexosephosphate aminotransferase; EC 2.6.1.16 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
38% identity, 72% coverage: 172:602/602 of query aligns to 278:717/717 of P14742
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 active site, For GATase activity
Q06210 Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1; D-fructose-6-phosphate amidotransferase 1; Glutamine:fructose-6-phosphate amidotransferase 1; GFAT 1; GFAT1; Hexosephosphate aminotransferase 1; EC 2.6.1.16 from Homo sapiens (Human) (see paper)
38% identity, 69% coverage: 186:602/602 of query aligns to 274:699/699 of Q06210
2zj4A Isomerase domain of human glucose:fructose-6-phosphate amidotransferase (see paper)
39% identity, 59% coverage: 245:602/602 of query aligns to 2:365/365 of 2zj4A
- active site: E238 (= E474), K242 (= K478), E245 (= E481), H261 (= H497), K360 (= K597)
- binding 2-deoxy-2-amino glucitol-6-phosphate: T60 (= T298), S61 (≠ A299), S105 (= S343), Q106 (= Q344), S107 (= S345), T110 (= T348), V156 (= V394), A157 (= A395), K242 (= K478), E245 (= E481)
2zj3A Isomerase domain of human glucose:fructose-6-phosphate amidotransferase (see paper)
39% identity, 59% coverage: 245:602/602 of query aligns to 2:365/365 of 2zj3A
- active site: E238 (= E474), K242 (= K478), E245 (= E481), H261 (= H497), K360 (= K597)
- binding 6-O-phosphono-alpha-D-glucopyranose: T60 (= T298), S61 (≠ A299), S105 (= S343), Q106 (= Q344), S107 (= S345), T110 (= T348), V156 (= V394), A359 (= A596), K360 (= K597)
2v4mA The isomerase domain of human glutamine-fructose-6-phosphate transaminase 1 (gfpt1) in complex with fructose 6-phosphate
38% identity, 57% coverage: 245:590/602 of query aligns to 1:352/352 of 2v4mA
- active site: E237 (= E474), K241 (= K478), E244 (= E481), H260 (= H497)
- binding fructose -6-phosphate: T59 (= T298), S60 (≠ A299), S104 (= S343), Q105 (= Q344), S106 (= S345), T109 (= T348), A156 (= A395), S157 (= S396), K241 (= K478), E244 (= E481)
2pocB The crystal structure of isomerase domain of glucosamine-6-phosphate synthase from candida albicans (see paper)
38% identity, 57% coverage: 248:590/602 of query aligns to 2:352/352 of 2pocB
- active site: E236 (= E474), K240 (= K478), E243 (= E481), H259 (= H497)
- binding 6-O-phosphono-beta-D-glucopyranose: C55 (= C296), T57 (= T298), S58 (≠ A299), S102 (= S343), Q103 (= Q344), S104 (= S345), T107 (= T348), E243 (= E481)
- binding uridine-diphosphate-n-acetylglucosamine: R24 (vs. gap), G36 (≠ A281), G126 (= G367), V128 (= V369), S136 (≠ A377), T139 (≠ S380), C141 (≠ V382), G142 (≠ I383), V143 (≠ L384), H144 (≠ P385)
1xfgA Glutaminase domain of glucosamine 6-phosphate synthase complexed with l-glu hydroxamate (see paper)
49% identity, 38% coverage: 2:232/602 of query aligns to 1:232/238 of 1xfgA
- active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W74), N98 (= N98), G99 (= G99)
- binding glutamine hydroxamate: C1 (= C2), R73 (= R73), W74 (= W74), T76 (= T76), H86 (= H86), N98 (= N98), G99 (= G99), D123 (= D123)
1xffA Glutaminase domain of glucosamine 6-phosphate synthase complexed with glutamate (see paper)
49% identity, 38% coverage: 2:232/602 of query aligns to 1:232/238 of 1xffA
- active site: C1 (= C2), R26 (= R27), G27 (= G28), W74 (= W74), N98 (= N98), G99 (= G99)
- binding glutamic acid: C1 (= C2), R73 (= R73), W74 (= W74), T76 (= T76), H86 (= H86), N98 (= N98), G99 (= G99), D123 (= D123)
2putA The crystal structure of isomerase domain of glucosamine-6-phosphate synthase from candida albicans (see paper)
36% identity, 57% coverage: 248:590/602 of query aligns to 2:339/339 of 2putA
- active site: E236 (= E474), K240 (= K478), E243 (= E481)
- binding fructose -6-phosphate: C55 (= C296), T57 (= T298), S102 (= S343), Q103 (= Q344), S104 (= S345), T107 (= T348), A154 (= A395), S155 (= S396), K240 (= K478)
- binding uridine-diphosphate-n-acetylglucosamine: R24 (vs. gap), G36 (≠ A281), G126 (= G367), V128 (= V369), S136 (≠ A377), T139 (≠ S380), C141 (≠ V382), G142 (≠ I383), V143 (≠ L384), H144 (≠ P385)
2puvA The crystal structure of isomerase domain of glucosamine-6-phosphate synthase from candida albicans (see paper)
36% identity, 57% coverage: 248:590/602 of query aligns to 3:338/338 of 2puvA
- active site: E237 (= E474), K241 (= K478), E244 (= E481)
- binding 5-amino-5-deoxy-1-o-phosphono-d-mannitol: C56 (= C296), T58 (= T298), S103 (= S343), Q104 (= Q344), S105 (= S345), T108 (= T348), A155 (= A395), E244 (= E481)
- binding uridine-diphosphate-n-acetylglucosamine: R25 (≠ D273), G37 (≠ F285), G127 (= G367), V129 (= V369), S137 (≠ A377), T140 (≠ S380), C142 (≠ V382), G143 (≠ I383), V144 (≠ L384), H145 (≠ P385)
Query Sequence
>GFF1006 FitnessBrowser__Phaeo:GFF1006
MCGIVGVLGNHEAAPILVEALKRLEYRGYDSAGIATVNGGALDRRRAVGKLVNLSDLLVH
DPLPGKSGIGHTRWATHGAPTVGNAHPHRAGGVAVVHNGIIENFKELREELAQKGITFAT
ETDTETVALLCQSLIGDGKTPVEAARETVQQLEGAFALAFLFEGEADLMIAARKGSPLAI
GHGDGEMFVGSDAIALAPFTDQITYLEEGDFAVLTRNSLEIRDQRGAIANREKRQIQLAN
ARVDKDGHKHFMAKEIAEQPVAIDRALKAYMGDDGQLSLPAELDFTKIERLTMVACGTAF
YACMVAKYWFGQIARMPVEVDVASEFRYREPPVPEKTLALFVSQSGETADTLAALRYMEG
KAQQIVGLVNVPESSIARESDVILPLHAGPEISVASTKAFTCQLSILLLLALRAAEQRGL
PLPDGMPADLRALPGLIHQSLAAEKQITASARGLAEARDIIFLGRGQLYPLALEGALKLK
ELSYIHAEGYASGELKHGPIALIDEKVPVVVMAPRDALFDKTVSNMQEVMARGGKVILIT
DAEGAKIAGDGTHEVIVMPQVPDSLAPILYAVPAQQIAYYTAIAKGTDVDQPRNLAKSVT
VE
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory