SitesBLAST

G51 (= G85) mutation to S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
A54 (≠ Q88) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
K81 (= K115) mutation to R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.
S202 (≠ C246) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ V378) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (= M388) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
24% identity, 77% coverage: 56:389/436 of query aligns to 22:343/364 of 1ng3A

query
sites
1ng3A

H

Y

L

L

A

A

E

K

A

E

G

N

R

K

S

N

V

T

I

A

L

L

L
x
F

E
|
E

A
x
S

N

G

R

T

V

M

G

G

F

G

G
x
R

A
x
T

S
x
T

G

S

R

A

N
x
A

G
x
A

G
|
G

Q
x
M

L

L

G

G

S

A

G

H

Q

A

R

E

M

C

E

E

Q

-

D

-

G

-

L

-

E

-

S

-

L

-

M

-

G

-

E

E

P

R

D

D

A

A

A

-

K

-

L

F

W

F

H

D

L

F

A

A

E

M

D

H

A

S

K

Q

D

R

L

L

V

Y

K

K

S

G

L

L

-

G

-

E

-

L

-

Y

-

A

I

L

A

S

R

G

H

V

D

D

I

I

D
x
R

C

Q

H

H

L

-

K

N

P

G

G

G

I

M

A

F

H

K

A

L

C

A

F

F

S

S

K

E

S

E

D

D

V

V

S

L

H

-

E

Q

H

L

R

R

Y

Q

V

M

E

D

H

D

L

L

Q

D

T

S

R

V

Y

S

G

W

Y

Y

G

S

D

K

I

E

T

E

A

V

L

L

D

E

K

K

A

E

A

P

L

Y

Q

-

A

-

I

-

C

-

P

A

S

S

P

G

A

D

Y

I

V

F

G

G

G

A

S

S

L

F

D

I

M

Q

G

D

A

D

G

V

H

H

L

V

H

E

P

P

L

Y

A

F

Y

V

A

C

L

K

G

A

L

Y

A

V

R

K

A

A

A

K

A

M

A

L

G

G

V

A

Q

E

I

I

C

F

E

E

G

H

S

T

E

P

V
|
V

L

L

D

H

I

V

E

E

-

R

D

D

G

G

A

E

Q

A

I

L

R

F

L

I

R

K

T

T

A

P

E

S

G

G

Q

D

V

V

T

W

A

A

D

N

H

H

L

V

I

V

L

V

A

A

C
x
S

N
x
G

G

V

Y
x
W

L

S

G

G

G

M

L
x
F

N

F

R

K

Q

Q

V

L

A

G

A

-

R

-

V

-

M

-

P

-

I

L

N

N

F

-

I

-

A

-

A

A

T

F

E

L

P

P

L

V

G

K

A

G

D

E

A

C

A

L

Q

S

V

V

L

W

A

N

R

D

D

D

V

I

A

P

V

L

A

T

D

K

S

T

K

L

F

Y

V

H

V

D

N

H

-

C

Y
|
Y

F

I

R

V

L

P

S

R

H

K

D

S

G

G

R
|
R

L

L

L

V

F

V

G

G

G

A

G

T

E

M

S

K

Y

-

G

-

Y

-

R

-

F

-

P

P

S

G

D

D

I

W

A

S

A

E

T

T

-

P

-

D

-

L

-

G

-

L

-

E

-

S

V

V

R

M

K

K

P

K

M

A

T

K

E

T

I

M

F

L

P

P

H

P

L

I

R

Q

D

N

V

M

K

K

I

V

D

D

Y

R

A

F

W

W

G

A

G
|
G

T

L

L
x
R

A

P

I

G

T

T

L

K

K

D

R

G

M

K

P

P

Y

Y

L

I

A

G

R

R

L

H

G

P

P

E

N

D

-

S

-

R

I

I

L

L

S

F

A

A

S

A

G

G

Y
x
H

S

F

G
x
R

H
x
N

G
|
G

V
x
I

G

L

T

L

A

A

T

P

H

A

A

T

G

G

Q

A

L

L

M

I

A

S

active site: A47 (≠ G81), G48 (= G82), M49 (≠ Q83)
binding acetylamino-acetic acid: Y246 (= Y297), R302 (≠ L350), R329 (≠ G375)
binding flavin-adenine dinucleotide: F33 (≠ L67), E34 (= E68), S35 (≠ A69), R41 (≠ G75), T42 (≠ A76), T43 (≠ S77), A46 (≠ N80), A47 (≠ G81), G48 (= G82), M49 (≠ Q83), V174 (= V219), S202 (≠ C246), G203 (≠ N247), W205 (≠ Y249), F209 (≠ L253), G300 (= G348), R302 (≠ L350), H327 (≠ Y373), R329 (≠ G375), N330 (≠ H376), G331 (= G377), I332 (≠ V378)
binding phosphate ion: R89 (≠ D128), R254 (= R305)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 15

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
24% identity, 77% coverage: 56:389/436 of query aligns to 22:343/369 of O31616

query
sites
O31616

H

Y

L

L

A

A

E

K

A

E

G

N

R

K

S

N

V

T

I

A

L

L

L

F

E
|
E

A
x
S

N

G

R

T

V

M

G

G

F

G

G

R

A
x
T

S
x
T

G

S

R

A

N

A

G
x
A

G
|
G

Q
x
M

L

L

G
|
G

S

A

G

H

Q
x
A

R

E

M

C

E

E

Q

-

D

-

G

-

L

-

E

-

S

-

L

-

M

-

G

-

E

E

P

R

D

D

A

A

A

-

K

-

L

F

W

F

H

D

L

F

A

A

E

M

D

H

A

S

K

Q

D

R

L

L

V

Y

K

K

S

G

L

L

-

G

-

E

-

L

-

Y

-

A

I

L

A

S

R

G

H

V

D

D

I

I

D

R

C

Q

H

H

L

-

K

N

P

G

G

G

I

M

A

F

H

K

A

L

C

A

F

F

S

S

K

E

S

E

D

D

V

V

S

L

H

-

E

Q

H

L

R

R

Y

Q

V

M

E

D

H

D

L

L

Q

D

T

S

R

V

Y

S

G

W

Y

Y

G

S

D

K

I

E

T

E

A

V

L

L

D

E

K

K

A

E

A

P

L

Y

Q

-

A

-

I

-

C

-

P

A

S

S

P

G

A

D

Y

I

V

F

G

G

G

A

S

S

L

F

D

I

M

Q

G

D

A

D

G

V

H

H

L

V

H

E

P

P

L

Y

A

F

Y

V

A

C

L

K

G

A

L

Y

A

V

R

K

A

A

A

K

A

M

A

L

G

G

V

A

Q

E

I

I

C

F

E

E

G

H

S

T

E

P

V
|
V

L

L

D

H

I

V

E

E

-

R

D

D

G

G

A

E

Q

A

I

L

R

F

L

I

R

K

T

T

A

P

E

S

G

G

Q

D

V

V

T

W

A

A

D

N

H

H

L

V

I

V

L

V

A

A

C

S

N

G

G

V

Y

W

L

S

G

G

G

M

L

F

N

F

R

K

Q

Q

V

L

A

G

A

-

R

-

V

-

M

-

P

-

I

L

N

N

F

-

I

-

A

-

A

A

T

F

E

L

P

P

L

V

G

K

A

G

D

E

A

C

A

L

Q

S

V

V

L

W

A

N

R

D

D

D

V

I

A

P

V

L

A

T

D

K

S

T

K

L

F

Y

V

H

V

D

N
x
H

-

C

Y

Y

F

I

R

V

L

P

S

R

H

K

D

S

G

G

R

R

L

L

L

V

F

V

G

G

G

A

G

T

E

M

S

K

Y

-

G

-

Y

-

R

-

F

-

P

P

S

G

D

D

I

W

A

S

A

E

T

T

-

P

-

D

-

L

-

G

-

L

-

E

-

S

V

V

R

M

K

K

P

K

M

A

T

K

E

T

I

M

F

L

P

P

H

A

L

I

R

Q

D

N

V

M

K

K

I

V

D

D

Y

R

A

F

W

W

G

A

G

G

T

L

L
x
R

A

P

I

G

T

T

L

K

K

D

R

G

M

K

P

P

Y

Y

L

I

A

G

R

R

L

H

G

P

P

E

N

D

-

S

-

R

I

I

L

L

S

F

A

A

S

A

G

G

Y
x
H

S
x
F

G
x
R

H
x
N

G
|
G

V
x
I

G
x
L

T

L

A

A

T

P

H

A

A

T

G

G

Q

A

L

L

M

I

A

S

ES 34:35 (≠ EA 68:69) binding FAD
TT 42:43 (≠ AS 76:77) binding FAD
AGM 47:49 (≠ GGQ 81:83) binding FAD
G51 (= G85) mutation to R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; mutation to S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
A54 (≠ Q88) mutation to R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.
V174 (= V219) binding FAD
H244 (≠ N296) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (≠ L350) binding substrate
327:333 (vs. 373:379, 14% identical) binding FAD
R329 (≠ G375) binding substrate

Sites not aligning to the query:

14:15 binding FAD

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
24% identity, 77% coverage: 56:389/436 of query aligns to 22:343/364 of 3if9A

query
sites
3if9A

H

Y

L

L

A

A

E

K

A

E

G

N

R

K

S

N

V

T

I

A

L

L

L

F

E
|
E

A
x
S

N

G

R

T

V

M

G

G

F

G

G

R

A
x
T

S
x
T

G

S

R

A

N
x
A

G
x
A

G
|
G

Q
x
M

L

L

G

S

S

A

G

H

Q

R

R

E

M

C

E

E

Q

-

D

-

G

-

L

-

E

-

S

-

L

-

M

-

G

-

E

E

P

R

D

D

A

A

A

-

K

-

L

F

W

F

H

D

L

F

A

A

E

M

D

H

A

S

K

Q

D

R

L

L

V

Y

K

K

S

G

L

L

-

G

-

E

-

L

-

Y

-

A

I

L

A

S

R

G

H

V

D

D

I

I

D

R

C

Q

H

H

L

-

K

N

P

G

G

G

I

M

A

F

H

K

A

L

C

A

F

F

S

S

K

E

S

E

D

D

V

V

S

L

H

-

E

Q

H

L

R

R

Y

Q

V

M

E

D

H

D

L

L

Q

D

T

S

R

V

Y

S

G

W

Y

Y

G

S

D

K

I

E

T

E

A

V

L

L

D

E

K

K

A

E

A

P

L

Y

Q

-

A

-

I

-

C

-

P

A

S

S

P

G

A

D

Y

I

V

F

G

G

G

A

S

S

L

F

D

I

M

Q

G

D

A

D

G

V

H

H

L

V

H

E

P

P

L

Y

A

F

Y

V

A

C

L

K

G

A

L

Y

A

V

R

K

A

A

A

K

A

M

A

L

G

G

V

A

Q

E

I

I

C

F

E

E

G

H

S

T

E
x
P

V
|
V

L

L

D

H

I

V

E

E

-

R

D

D

G

G

A

E

Q

A

I

L

R

F

L

I

R

K

T

T

A

P

E

S

G

G

Q

D

V

V

T

W

A

A

D

N

H

H

L

V

I

V

L

V

A

A

C
x
S

N
x
G

G

V

Y
x
W

L

S

G

G

G

M

L
x
F

N

F

R

K

Q

Q

V

L

A

G

A

-

R

-

V

-

M

-

P

-

I

L

N

N

F

-

I

-

A

-

A

A

T

F

E

L

P

P

L

V

G

K

A

G

D

E

A

C

A

L

Q

S

V

V

L

W

A

N

R

D

D

D

V

I

A

P

V

L

A

T

D

K

S

T

K

L

F

Y

-

H

V

D

V

A

N

C

Y
|
Y

F

I

R

V

L

P

S

R

H

K

D

S

G

G

R

R

L

L

L

V

F

V

G

G

G

A

G

T

E

M

S

K

Y

-

G

-

Y

-

R

-

F

-

P

P

S

G

D

D

I

W

A

S

A

E

T

T

-

P

-

D

-

L

-

G

-

L

-

E

-

S

V

V

R

M

K

K

P

K

M

A

T

K

E

T

I

M

F

L

P

P

H

A

L

I

R

Q

D

N

V

M

K

K

I

V

D

D

Y

R

A

F

W

W

G

A

G
|
G

T

L

L
x
R

A

P

I

G

T

T

L

K

K

D

R

G

M

K

P

P

Y

Y

L

I

A

G

R

R

L

H

G

P

P

E

N

D

-

S

-

R

I

I

L

L

S

F

A

A

S

A

G

G

Y
x
H

S
x
F

G
x
R

H
x
N

G
|
G

V
x
I

G

L

T

L

A

A

T

P

H

A

A

T

G

G

Q

A

L

L

M

I

A

S

active site: A47 (≠ G81), G48 (= G82), M49 (≠ Q83)
binding flavin-adenine dinucleotide: E34 (= E68), S35 (≠ A69), T42 (≠ A76), T43 (≠ S77), A46 (≠ N80), A47 (≠ G81), G48 (= G82), M49 (≠ Q83), P173 (≠ E218), V174 (= V219), S202 (≠ C246), G203 (≠ N247), W205 (≠ Y249), F209 (≠ L253), G300 (= G348), R302 (≠ L350), H327 (≠ Y373), F328 (≠ S374), R329 (≠ G375), N330 (≠ H376), G331 (= G377), I332 (≠ V378)
binding glycolic acid: Y246 (= Y297), R302 (≠ L350), R329 (≠ G375)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 15

2gagB Heteroteterameric sarcosine: structure of a diflavin metaloenzyme at 1.85 a resolution (see paper)
25% identity, 82% coverage: 40:395/436 of query aligns to 21:373/403 of 2gagB

query
sites
2gagB

D

D

V

A

C

I

I

I

V

V

G
|
G

G

G

G
|
G

Y
x
G

T
x
H

G

G

L

L

S

A

A

T

A

A

L

Y

H

F

L

L

A

A

E

K

A

N

G

H

-

G

-

I

R

T

S

N

V

V

I

A

L

V

L

L

E
|
E

A
x
K

N

G

R

W

V

L

G

A

F

G

G
|
G

A
x
N

S
x
M

G
x
A

R
|
R

N
|
N

G
x
T

Q
x
I

L

I

G
x
R

S

S

G

N

Q

Y

R

L

M

W

E

D

Q

-

D

-

G

-

L

-

E

E

S

S

L

A

M

G

G

I

E

Y

P

E

D

K

A

S

A

L

K

K

L

L

W

W

-

E

H

Q

L

L

A

P

E

E

D

D

A

L

K

E

-

Y

D

D

L

F

V

L

K

F

S

S

L

-

I

-

A

-

R

-

H

-

D

-

I

-

D

-

C

-

H

-

L

-

K

Q

P

R

G

G

I

V

A

L

H

N

A

L

C

A

F

H

S

T

K

L

S

G

D

D

V

V

S

R

H

E

E

S

H

V

R

R

Y

R

V

V

E

E

H

-

L

-

Q

A

T

N

R

K

Y

L

G

N

Y

G

G

V

D

D

I

A

T

E

A

W

L

L

D

D

K

P

A

S

A

Q

L

V

Q

K

A

E

I

A

C

C

P

P

-

I

-

N

-

T

-

S

-

D

-

I

S

R

P

Y

A

P

Y

V

V

M

G

G

G

A

S

T

L

W

D

Q

M

P

G

R

A

A

G

G

H

I

L

A

H
x
K

P
x
H

L

D

A

H

Y

V

A

A

L

W

G

A

L

F

A

A

R

R

A

K

A

A

A

N

A

E

A

M

G

G

V

V

Q

D

I

I

C

I

E

Q

G

N

S

C

E

E

V
|
V

L

T

D

G

-

F

I

I

E

K

D

D

G

G

A

E

Q

K

I

V

R

T

-

G

L

V

R

K

T

T

A

T

E

R

G

G

Q

T

V

I

T

H

A

A

D

G

H

K

L

V

I

A

L

L

A

A

C
x
G

N
x
A

G

G

Y
x
H

L

S

G

S

G

V

L
|
L

N

-

R

A

Q

E

V

M

A

A

A

G

R

F

V

E

M

L

P

P

I

I

N

Q

N

-

F

-

I

-

A

-

T

S

E

H

P

P

L
|
L

G

Q

A

A

D

L

A
x
V

A

S

Q

E

V

L

L

F

A

E

R

P

-

V

D

H

V

P

A

T

V

V

A

V

D
x
M

S

S

K

N

F

H

V

I

V

H

N

V

Y
|
Y

F

V

R

S

L

Q

S

A

H

H

D
x
K

G

G

R
x
E

L

L

L

V

F

M

G

G

G

A

G

G

-

I

-

D

-

S

-

Y

E

N

S

G

Y

Y

G

G

Y

Q

R

R

F

G

P

A

S

F

D

H

I

V

A

I

A

Q

T

E

V

Q

R

M

K

A

P

A

M

A

T

V

E

E

I

L

F

F

P

P

H

I

L

F

R

A

D

R

V

A

K

H

I

V

D

L

Y
x
R

A

T

W
|
W

G

G

T

I

L

V

A

D

I

T

T

T

L

M

K

D

R

A

M

S

P

P

Y

I

L

I

A

S

R

K

L

T

G

P

-

I

P

Q

N

N

I

L

L

Y

S

V

A

N

S

C

G

G

Y

W

S
x
G

G
x
T

H
x
G

G
|
G

V
x
F

G
x
K

T

G

A

T

T

P

H

G

A

A

G

G

Q

F

L

T

M

L

A

A

L

H

A

T

V

I

A

A

G

N

D

D

active site: A61 (≠ G78), T64 (≠ G81), T65 (≠ G82)
binding flavin-adenine dinucleotide: G26 (= G45), G28 (= G47), G29 (≠ Y48), H30 (≠ T49), E51 (= E68), K52 (≠ A69), G58 (= G75), N59 (≠ A76), M60 (≠ S77), R62 (= R79), N63 (= N80), T64 (≠ G81), I66 (≠ Q83), V195 (= V219), G224 (≠ C246), A225 (≠ N247), H227 (≠ Y249), L231 (= L253), L246 (= L274), G352 (≠ S374), T353 (≠ G375), G354 (≠ H376), G355 (= G377), F356 (≠ V378), K357 (≠ G379)
binding flavin mononucleotide: A61 (≠ G78), R62 (= R79), H171 (≠ P195), V250 (≠ A278), E278 (≠ R305), R321 (≠ Y344), W323 (= W346)
binding 2-furoic acid: T64 (≠ G81), I66 (≠ Q83), R68 (≠ G85), M263 (≠ D290), Y270 (= Y297), K357 (≠ G379)
binding sulfite ion: K170 (≠ H194), K276 (≠ D303)

Sites not aligning to the query:

binding 2-furoic acid: 401

Q63342 Dimethylglycine dehydrogenase, mitochondrial; ME2GLYDH; EC 1.5.8.4 from Rattus norvegicus (Rat) (see 2 papers)
24% identity, 66% coverage: 28:313/436 of query aligns to 32:307/857 of Q63342

query
sites
Q63342

A

S

P

P

F

P

A

S

P

P

L

P

Q

E

G

W

E

K

A

D

R

R

A

A

D

E

V

T

C

V

I

I

V

I

G

G

Y
x
C

T
x
V

G

G

L

V

S

S

A

L

A

A

L

Y

H

H

L

L

A

A

E

K

A

A

G

G

-

M

R

R

S

D

V

V

I

V

L

L

E
|
E

A
x
K

N

S

R

E

V

L

G

T

F

A

G
|
G

A
x
S

S
x
T

G
x
W

R
x
H

N
x
A

G
x
A

G
|
G

Q
x
L

L

-

G

-

S

-

G

-

Q

T

R

T

M

Y

E

F

Q

H

D

P

G

G

L

I

E

N

S

L

L

K

M

K

G

I

E

H

P

Y

D

D

A

S

A

I

K

K

L

L

W

Y

H

E

L

R

A

L

E

E

D

E

A

E

K

T

D

G

L

Q

V

V

K

-

S

-

L

-

I

-

A

-

R

-

H

-

D

-

I

V

D

G

C

F

H

H

L

-

K

Q

P

P

G

G

I

S

A

I

H

R

A

L

C

A

F

T

S

T

K

P

S

E

D

R

V

V

S

D

H

-

E

E

H

F

R

K

Y

Y

V

-

E

Q

H

M

L

T

Q

R

T

T

R

N

Y

W

G

H

Y

A

G

T

D

E

I

Q

T

Y

A

I

L

I

D

E

K

P

A

E

A

K

L

I

Q

H

A

E

I

L

C

F

P

P

-

L

-

N

S

M

P

D

A

K

Y

I

V

L

G

A

G

G

S

L

L

Y

D

N

M

P

G

G

A

D

G

G

H

H

L

I

H

D

P

P

L

Y

A

S

Y

L

A

T

L

M

G

A

L

L

A

A

R

T

A

G

A

A

A

R

A

K

A

Y

G

G

V

V

Q

L

I

L

C

K

E

Y

G

P

S

A

E

P

V
|
V

L

T

D

S

I

L

E

K

-

P

-

R

-

P

D

D

G

G

A

T

Q

W

I

-

R

D

L

V

R

E

T

T

A

P

E

Q

G

G

Q

S

V

V

T

R

A

A

D

N

H

R

L

I

I

V

L

N

A

A

C

A

N

G

G

F

Y
x
W

L

A

G

R

G

E

L

V

N

G

R

K

Q

M

V

I

A

G

A

L

R

D

-

H

-

P

V

L

M

I

P

P

I

V

N

Q

N

H

F

Q

I

Y

A

V

T

T

E

S

P

T

L

I

G

-

A

-

D

P

A

E

A

V

Q

K

V

A

L

L

A

K

R

R

D

E

V

L

A

P

V

V

A

L

D

R

S

D

K

L

F

E

V

G

V

S

N

Y

Y

Y

F

L

R

R

L

Q

S

E

H

R

D

D

G

G

R

-

L

L

F

F

G

G

G

P

G

Y

E

E

S

S

CV 52:53 (≠ YT 48:49) binding FAD
EK 73:74 (≠ EA 68:69) binding FAD
80:88 (vs. 75:83, 22% identical) binding FAD
H84 (≠ R79) modified: Tele-8alpha-FAD histidine
V212 (= V219) binding FAD
W244 (≠ Y249) binding FAD

Sites not aligning to the query:

390:395 binding FAD
573:575 binding (6S)-5,6,7,8-tetrahydrofolate
669 binding (6S)-5,6,7,8-tetrahydrofolate
676:678 binding (6S)-5,6,7,8-tetrahydrofolate
737 binding (6S)-5,6,7,8-tetrahydrofolate

1y56B Crystal structure of l-proline dehydrogenase from p.Horikoshii (see paper)
19% identity, 77% coverage: 54:389/436 of query aligns to 20:348/374 of 1y56B

query
sites
1y56B

A

A

L

H

H

E

L

L

A

A

E

K

A

R

G

G

R

E

S

E

V

V

I

T

L

V

L
x
I

E
|
E

A
x
K

N

R

R

F

V

I

G

G

F

S

G

G

A
x
S

S
x
T

G
x
F

R
|
R

N

-

G

-

Q

-

L
x
C

G
|
G

S
x
T

G
|
G

Q

I

R

R

M

Q

E

Q

Q

F

D

N

G

D

L

E

E

A

S

N

L

V

M

R

G

V

E

M

P

K

D

R

A

S

A

V

K

E

L

L

W

W

H

K

L

K

A

Y

E

S

D

E

A

E

K

Y

D

G

L

F

V

-

K

-

S

-

L

-

I

-

A

-

R

-

H

-

D

-

I

-

D

-

C

S

H

F

L

K

K

Q

P

T

G

G

I

Y

A

L

H

F

A

L

C

L

F

Y

S

D

K

D

S

E

D

E

V

V

S

K

H

T

E

F

H

K

R

R

Y

N

V

I

E

E

H

-

L

I

Q

Q

T

N

R

K

Y

F

G

G

Y

V

G

P

D

T

-

K

-

L

I

I

T

T

A

P

L

E

D

E

K

A

A

K

A

E

L

I

Q

V

A

P

I

L

C

L

P

D

S

I

P

S

A

E

Y

V

V

I

G

A

S

S

L

W

D

N

M

P

G

T

A

D

G

G

H

K

L

A

H

D

P

P

L

F

A

E

Y

A

A

T

L

T

G

A

L

F

A

A

R

V

A

K

A

A

A

K

A

E

A

Y

G

G

V

A

Q

K

I

L

C

L

E

E

G

Y

S

T

E
|
E

V
|
V

-

K

-

G

L

F

D

L

I

I

E

E

D

N

G

N

A

E

Q

I

I

K

R

G

L

V

R

K

T

T

A

N

E

K

G

G

Q

I

V

I

T

K

A

T

D

G

H

I

L

V

I

V

L

N

A

A

C
x
T

N
|
N

G

A

Y
x
W

L

A

G

N

G

L

L

I

N

N

R

A

Q

M

V

A

A

G

A

I

R

K

-

T

-

K

-

I

-

P

V

I

M

E

P

P

I

Y

N

K

N
x
H

F

Q

I

A

A

V

A

I

T

T

E

Q

P

P

L

I

G

K

A

R

D

G

A

T

A

I

Q

N

V
x
P

L

M

A

V

R

I

D

S

V

F

A

K

V

Y

A

G

D

H

S

A

K

-

F

Y

V

L

V

T

N

Q

Y

T

F

F

R

H

L

G

S

G

H

I

D
x
I

G

G

R

G

L

I

L

G

F

Y

G

E

G

I

G

G

E

P

S

T

Y

Y

G

D

Y

L

R

T

F

P

P

T

S

Y

D

E

I

F

A

L

A

R

T

E

V

V

R

S

K

Y

P

Y

M

F

T

T

E

K

I

I

F

I

P

P

H

A

L

L

R

K

D

N

V

L

K

L

I

I

D

L

Y
x
R

A

T

W
|
W

G

A

G
|
G

T
x
Y

L
x
Y

A

A

I

K

T

T

L

P

K

D

R

S

M

N

P

P

Y

A

L

I

A

G

R

R

L

I

G

E

P

E

-

L

-

N

N

D

I

Y

L

Y

S

I

A

A

S

A

G

G

Y

F

S

S

G
|
G

H
|
H

G
|
G

V
x
F

G
x
M

T

M

A

A

T

P

H

A

A

V

G

G

Q

E

L

M

M

V

A

A

active site: F44 (≠ G78), G47 (= G85), T48 (≠ S86), H224 (≠ N266), P239 (≠ V281), G305 (= G348), M338 (≠ G379)
binding flavin-adenine dinucleotide: I33 (≠ L67), E34 (= E68), K35 (≠ A69), S42 (≠ A76), T43 (≠ S77), R45 (= R79), C46 (≠ L84), G47 (= G85), G49 (= G87), E170 (= E218), V171 (= V219), T200 (≠ C246), N201 (= N247), W203 (≠ Y249), G305 (= G348), Y306 (≠ T349), Y307 (≠ L350), G334 (= G375), H335 (= H376), G336 (= G377), F337 (≠ V378), M338 (≠ G379)
binding flavin mononucleotide: F44 (≠ G78), R45 (= R79), I260 (≠ D303), R301 (≠ Y344), W303 (= W346)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 11, 13, 14, 15

Q8GAI3 4-methylaminobutanoate oxidase (formaldehyde-forming); MABO; Demethylating gamma-N-methylaminobutyrate oxidase; Gamma-N-methylaminobutyrate oxidase 1; EC 1.5.3.19 from Paenarthrobacter nicotinovorans (Arthrobacter nicotinovorans) (see paper)
24% identity, 90% coverage: 32:422/436 of query aligns to 21:412/824 of Q8GAI3

query
sites
Q8GAI3

P

P

L

L

Q

P

G

T

E

H

A

V

R

R

A

-

D

-

V

T

C

V

I

V

V

V

G

G

Y

I

T

I

G

G

L

A

S

S

A

I

A

A

L

Y

H

H

L

L

A

S

E

A

A

A

G

G

R

E

S

N

-

D

V

T

I

L

L

L

E

E

A

S

N

N

R

V

V

L

G

G

F

S

G

G

A

T

S

S

G
x
W

R
x
H

N

A

G

A

G

G

Q

-

L

L

G

V

S

T

G

G

Q

A

R

R

M

-

E

-

Q

-

D

-

G

-

L

-

E

-

S

-

L

-

M

-

G

-

E

-

P

-

D

G

A

T

K

T

L

M

W

T

H

K

L

L

A

A

E

K

D

Y

A

G

K

L

D

D

L

F

V

Y

K

S

S

R

L

L

I

E

A

Q

R

M

H

S

D

G

I

L

D

D

C

V

H

S

L

F

K

Q

P

R

G

C

I

G

A

S

H

L

A

S

C

V

F

A

S

R

K

T

S

A

D

G

V

R

S

V

H

D

E

E

H

L

R

L

Y

Y

V

A

E

K

H

D

L

V

Q

A

T

D

R

Q

Y

Q

G

G

Y

V

G

-

D

R

I

T

T

E

A

W

L

L

D

T

K

E

A

D

A

R

L

Y

Q

K

A

E

I

L

C

W

P

P

S

L

P

A

A

T

Y

Y

-

S

-

G

V

V

G

A

G

G

S

A

L

L

D

L

M

L

-

P

G

D

A

D

G

G

H

H

L

I

H

N

P

P

L

G

A

H

Y

A

A

T

L

V

G

A

L

L

A

A

R

K

A

L

A

A

A

H

A

S

A

L

G

G

V

T

Q

Q

I

I

C

R

E

E

G

N

S

V

E

A

V

V

L

H

D

K

I

V

-

L

-

R

E

Q

D

G

G

D

A

L

Q

V

I

V

R

G

L

V

R

L

T

T

A

D

E

Q

G

G

Q

I

V

V

T

H

A

C

D

D

H

R

L

V

I

I

L

L

A

A

C

C

N

-

G

-

Y

-

L

-

G

G

G

L

L

W

N

T

R

R

Q

D

V

L

A

A

R

-

V

T

M

A

P

G

I

V

N

K

N

V

F

P

I

L

A

Y

A

A

T

A

E

E

P

H

L

I

G

H

A

V

D

R

A

S

A

A

Q

E

V

I

L

D

A

G

R

A

D

V

V

P

A

E

V

L

A

P

D

V

S

Y

K

R

F

D

V

L

V

D

N

N

Y

S

F

Y

R

Y

L

I

S

R

H

H

D

E

-

A

G

G

R

R

L

L

F

V

G

G

G

A

G

F

E

E

S

P

Y

D

G

G

Y

L

R

P

F

R

P

P

S

V

D

E

I

E

A

I

-

P

-

S

-

N

-

G

-

F

-

A

-

E

-

F

-

G

-

P

-

E

-

W

-

E

-

H

-

F

A

A

T

P

V

I

R

R

K

A

P

K

M

A

T

E

E

G

I

V

F

V

P

P

H

A

L

L

R

A

D

S

V

A

K

G

I

F

D

D

Y

R

A

F

W

L

G

N

G

A

T

P

L

E

A

S

I

F

T

T

L

P

K

D

R

A

M

N

P

F

Y

A

L

V

A

G

R

E

L

T

G

S

P

E

-

L

-

S

N

N

I

L

L

F

S

V

A

A

S

A

G

G

Y

F

S

N

G

S

H

Q

G

G

V

I

G

I

T

F

A

A

T

P

H

G

A

I

G

G

Q

K

L

E

M

L

A

A

L

E

A

W

V

V

A

I

G

S

D

G

G

T

D

P

G

G

F

F

D

D

T

S

M

S

A

A

R

-

V

V

P

D

A

V

P

Q

A

R

F

F

P

S

G

G

G

H

A

Q

A

N

M

N

R

R

S

N

P

Y

L

L

L

K

A

A

W66 (≠ G78) mutation W->F,S: Contains a non-covalently bound FAD. Loss of enzyme activity.
H67 (≠ R79) mutation to A: Contains a non-covalently bound FAD. Exhibits about 10% of the wild-type enzyme activity.

Query Sequence

>GFF1136 FitnessBrowser__Phaeo:GFF1136
MALNLLYSNDRKGTYPNSWYAATATPLAPFAPLQGEARADVCIVGGGYTGLSAALHLAEA
GRSVILLEANRVGFGASGRNGGQLGSGQRMEQDGLESLMGEPDAAKLWHLAEDAKDLVKS
LIARHDIDCHLKPGIAHACFSKSDVSHEHRYVEHLQTRYGYGDITALDKAALQAICPSPA
YVGGSLDMGAGHLHPLAYALGLARAAAAAGVQICEGSEVLDIEDGAQIRLRTAEGQVTAD
HLILACNGYLGGLNRQVAARVMPINNFIAATEPLGADAAQVLARDVAVADSKFVVNYFRL
SHDGRLLFGGGESYGYRFPSDIAATVRKPMTEIFPHLRDVKIDYAWGGTLAITLKRMPYL
ARLGPNILSASGYSGHGVGTATHAGQLMALAVAGDGDGFDTMARVPAPAFPGGAAMRSPL
LALAMTWYALRDRLGI

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory