SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1137 FitnessBrowser__Phaeo:GFF1137 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P78061 Gamma-glutamylputrescine synthetase PuuA; Gamma-Glu-Put synthetase; Glutamate--putrescine ligase; EC 6.3.1.11 from Escherichia coli (strain K12) (see paper)
34% identity, 95% coverage: 24:451/452 of query aligns to 40:471/472 of P78061

query
sites
P78061

V

V

E

D

C

V

I

L

S

T

D

D

L

L

P

N

G

G

I

C

A

F

R

R

G

G

K

K

A

R

V

I

P

P

A

V

S

S

K

S

F

L

A

K

K

K

-

L

Q

E

D

K

Y

G

F

C

H

Y

L

F

P

P

D

A

S

S

I

V

F

F

Y

A

Q

M

T

D

I

I

T

L

G

G

D

N

W

V

A

V

E

E

A

E

A

A

D

G

D

L

D

G

G

Q

W

E

I

M

-

G

E

E

K

P

D

D

M

R

I

T

L

C

K

V

P

P

D

V

M

L

S

G

T

S

A

L

T

T

A

P

A

S

P

A

W

A

T

D

G

P

D

E

W

F

T

I

L

G

Q

Q

V

M

I

L

H

L

D

T

A

M

Y

V

D

D

R

E

D

D

H

G

K

A

P

P

I

F

P

D

F

V

S

E

P

P

R

R

N

N

V

V

L

L

K

N

R

R

V

L

V

W

Q

Q

L

Q

Y

L

H

R

D

Q

K

R

G

G

W

L

Q

F

P

P

V

V

A

A

P

V

E

E

M

L

E

E

F

F

F

Y

L

L

V

L

A

D

R

R

N

Q

I

R

D

D

P

A

A

E

R

G

E

Y

I

L

E

Q

P

P

M

P

M

C

-

A

-

P

G

G

R

T

S

D

G

D

R

R

P

N

A

T

A

Q

A

S

R

-

Q

Q

A

V

Y

Y

S

S

M

V

T

D

A

N

V

L

D

N

E

H

F

F

G

A

P

D

V

V

I

L

D

N

D

D

I

I

Y

D

D

E

F

L

A

A

E

Q

A

L

Q

Q

G

L

F

I

E

P

I

A

D

D

G

G

I

A

T

V

Q

A

E

E

G

A

G

S

A

P

G

G

Q

Q

L

F

E

E

I

I

N

N

L

L

R

Y

H

H

G

T

D

D

P

N

V

V

-

L

K

E

L

A

A

C

D

D

E

D

V

A

F

L

Y

A

F

L

K

K

R

R

L

L

I

V

R

R

E

L

A

M

A

A

L

E

R

K

H

H

D

K

C

M

F

H

A

A

T

T

F

F

M

M

A

A

K

K

P

P

I

Y

A

E

D

E

E

H

P

A

G

G

S

S

A

G

M

M

H
|
H

I

I

H

H

H

I

S

S

I

M

I

Q

D

N

M

-

E

N

S

R

G

G

D

E

N

N

I

V

F

L

S

S

G

D

P

A

Q

E

G

G

G

E

E

D

T

S

D

P

A

L

F

L

Y

K

H

K

F

M

I

L

G

A

G

G

L

M

Q

I

N

D

H

L

L

M

P

P

A

S

G

S

L

M

A

A

V

L

M

L

A

A

P

P

Y

N

V

V

N

N

S

S

Y

Y

R

R

Y

F

V

Q

K

P

E

G

Q

M

A

Y

A

V

P

P

I

T

N

Q

L

A

E

S

W

W

A

G

R

H

D

N

N

N

R

R

T

T

T

V

G

A

I

L

R
|
R

V

I

P

P

L

C

S

G

G

D

P

R

E

H

A

N

R

H

R

R

V

V

E

E

N

Y

R

R

I

V

A

A

G

G

M

A

D

D

C

A

N

N

P

P

Y

Y

L

L

G

V

I

M

A

A

L

A

S

I

L

F

A

A

C

G

G

I

Y

L

L

H

G

G

L

L

V

D

N

N

E

E

E

L

R

P

P

L

R

Q

K

E

Q

E

F

V

K

E

G

G

D

N

A

G

Y

L

A

E

G

Q

D

E

G

G

-

L

D

P

I

F

P

P

Q

I

V

R

M

Q

G

S

Q

D

A

A

L

L

D

G

L

E

F

F

E

I

E

E

A

N

S

D

A

H

L

L

H

R

E

R

V

Y

L

L

G

G

P

E

E

R

F

F

A

C

R

H

V

V

Y

Y

S

H

I

A

V

C

K

K

R

N

A

D

E

E

Y

L

D

L

E

Q

F

F

L

E

Q

R

V

L

I

I

S

T

P

E

W

T

E

E

R

I

E

E

H

W

L

M

L

L

L

K

N

N

H282 (= H262) mutation to N: Activity is impaired to 9% of wild-type.
R357 (= R338) mutation to Q: Activity is impaired to 3% of wild-type.

7tf9S L. Monocytogenes gs(14)-q-glnr peptide (see paper)
30% identity, 93% coverage: 28:449/452 of query aligns to 24:440/443 of 7tf9S

query
sites
7tf9S

I

F

S

T

D

D

L

I

P

L

G

G

I

I

A

I

R

K

G

N

K

V

A

E

V

I

P

P

A

V

S

S

K

Q

F

L

A

K

K

K

Q

A

D

-

Y

-

F

-

H

-

L

L

P

D

D

N

S

K

I

I

F

M

Y

F

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E

D

A

G

A

S

D

S

D

I

D

E

G

G

W
x
F

I
x
V

-
x
R

-
x
I

-

E

E

E

K

S

D

D

M

M

I

Y

L

L

K

F

P

P

D

D

M

L

S

D

T

T

A

W

T

V

A

V

A

F

P

P

W

W

T

T

G

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

V

M

I

I

H

C

D

D

A

I

Y

Y

D

N

R

P

D

D

H

M

K

T

P

P

I

F

P

A

F

G

S

D

P

P

R

R

N

A

V

N

L

L

K

K

R

R

V

V

V

L

Q

K

L

E

Y

M

H

E

D

E

K

L

G

G

W

F

Q

T

P

E

V

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

L

L

V

F

A

-

R

-

N

K

I

L

D

D

P

E

A

N

R

R

E

R

I

P

E

T

P

L

M

E

M

L

G

N

R

D

S

S

G

G

R

-

P

-

A

-

R

-

Q

G

A
x
Y

Y

F

S

D

M

L

T

A

A

P

V

T

D

D

E

L

F

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

F

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G

V

G

A

A

P

G

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

E

D

D

P

A

V

I

K

T

L

A

A

C

D

D

E

S

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

D

G

E

V

P
x
N

G
|
G

S
|
S

A
x
G

M

M

H
|
H

I

F

H

N

H

M

S

S

I

L

I

F

D

N

M

-

E

E

S

K

G

G

D

N

N

A

I

F

F

F

S

D

G

-

P

-

Q

E

G

S

G

G

E

E

-

L

-

E

-

L

T

S

D

Q

A

T

F

A

Y

Y

H

H

F

F

I

L

G

A

G

G

L

M

Q

L

N

K

H

H

L

A

P

R

A

G

G

Y

L

T

A

A

V

V

M

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

Y

L

V

V

K

P

E

G

Q

Y

A
x
E

A

A

P

P

I

C

N

Y

L

I

E

A

W

W

A

S

R

G

D

K

N

N

R

R

T

S

T

P

G

L

I

V

R

R

V

V

P

P

L

S

S

S

G

R

P

G

E

L

A

S

R

T

R

R

V

L

E
|
E

N

L

R

R

I

S

A

V

G

D

M

P

D

S

C

A

N

N

P

P

Y

Y

L

L

G

A

I

M

A

A

L

V

S

L

L

L

A

K

C

A

G

G

Y

L

L

S

G

G

L

I

V

K

N

D

E

E

E

L

R

T

P

P

R

P

K

A

Q

P

F

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

A

T

G

G

D

I

G

Y

D

D

I

L

P

P

Q

E

V

S

M

L

G

G

Q

H

A

A

L

L

D

I

L

E

F

L

E

E

E

K

A

N

S

E

A

I

L

I

H

K

E

D

V

G

L

L

G

G

P

E

E

H

F

I

A

F

R

E

V

H

Y

F

S

I

I
x
E

V

A

K

K

R

T

A
x
I

E

E

Y

C

D
|
D

E
x
M

F

F

L

R

Q

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding glutamine: E133 (= E143), Y155 (≠ A173), E188 (= E206), N239 (≠ P257), G240 (= G258), S241 (= S259), G242 (≠ A260), H244 (= H262), R297 (= R315), E303 (≠ A321)
binding magnesium ion: E131 (= E141), E133 (= E143), E188 (= E206), E195 (= E213), H244 (= H262), E332 (= E350)
binding : F59 (≠ W75), V60 (≠ I76), R61 (vs. gap), I62 (vs. gap), E418 (≠ I427), I422 (≠ A431), D425 (= D434), M426 (≠ E435)

7tenA Glutamine synthetase (see paper)
30% identity, 93% coverage: 28:449/452 of query aligns to 23:439/442 of 7tenA

query
sites
7tenA

I

F

S

T

D

D

L

I

P

L

G

G

I

I

A

I

R

K

G

N

K

V

A

E

V

I

P

P

A

V

S

S

K

Q

F

L

A

K

K

K

Q

A

D

-

Y

-

F

-

H

-

L

L

P

D

D

N

S

K

I

I

F

M

Y

F

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E

D

A

G

A

S

D

S

D

I

D

E

G

G

W

F

I

V

-

R

-

I

-

E

E

E

K

S

D

D

M

M

I

Y

L

L

K

F

P

P

D

D

M

L

S

D

T

T

A

W

T

V

A

V

A

F

P

P

W

W

T

T

G

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

V

M

I

I

H

C

D

D

A

I

Y

Y

D

N

R

P

D

D

H

M

K

T

P

P

I

F

P

A

F

G

S

D

P

P

R

R

N

A

V

N

L

L

K

K

R

R

V

V

V

L

Q

K

L

E

Y

M

H

E

D

E

K

L

G

G

W

F

Q

T

P

E

V

F

-

N

V

L

A
x
G

P
|
P

E
|
E

M

P

E
|
E

F

F

L

L

V

F

A

-

R

-

N

K

I

L

D

D

P

E

A

N

R

R

E

R

I

P

E

T

P

L

M

E

M

L

G

N

R

D

S

S

G

G

R

-

P

-

A

-

R

-

Q

G

A

Y

Y

F

S

D

M

L

T

A

A

P

V

T

D

D

E

L

F

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

F

F

E

E

I

I

D
x
E

G

A

I

S

T

H

Q

H

E
|
E

G

V

G

A

A

P

G

G

Q

Q

L

H

E
|
E

I

I

N
x
D

L
x
F

R
x
K

H
x
Y

G

E

D

D

P

A

V

I

K

T

L

A

A

C

D

D

E

S

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

D

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

F

H
x
N

H

M

S
|
S

I

L

I

F

D

N

M

-

E

E

S

K

G

G

D

N

N

A

I

F

F

F

S

D

G

-

P

-

Q

E

G

S

G

G

E

E

-

L

-

E

-

L

T

S

D

Q

A

T

F

A

Y

Y

H

H

F

F

I

L

G

A

G

G

L

M

Q

L

N

K

H

H

L

A

P

R

A

G

G

Y

L

T

A

A

V

V

M

T

A

N

P

P

Y

T

V

I

N

N

S

S

Y

F

R

K

R
|
R

Y

L

V

V

K

P

E

G

Q
x
Y

A
x
E

A

A

P

P

I

C

N

Y

L

I

E

A

W

W

A

S

R

G

D

K

N

N

R
|
R

T

S

T

P

G

L

I

V

R
|
R

V

V

P

P

L

S

S

S

G

R

P

G

E
x
L

A
x
S

R
x
T

R
|
R

V

L

E
|
E

N

L

R
|
R

I

S

A

V

G

D

M

P

D

S

C

A

N

N

P

P

Y

Y

L

L

G

A

I

M

A

A

L

V

S

L

L

L

A

K

C

A

G

G

Y

L

L

S

G

G

L

I

V

K

N

D

E

E

E

L

R

T

P

P

R

P

K

A

Q

P

F

V

K

D

G

R

D

N

A

I

Y

Y

-

G

-

M

-

N

-

E

-

R

-

E

-

A

A

T

G

G

D

I

G

Y

D

D

I

L

P

P

Q

E

V

S

M

L

G

G

Q

H

A

A

L

L

D

I

L

E

F

L

E

E

E

K

A

N

S

E

A

I

L

I

H

K

E

D

V

G

L

L

G

G

P

E

E

H

F

I

A

F

R

E

V

H

Y

F

S

I

I

E

V

A

K

K

R

T

A

I

E

E

Y

C

D

D

E

M

F

F

L

R

Q

T

V

A

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

L

binding adenosine-5'-diphosphate: G128 (≠ A139), P129 (= P140), E130 (= E141), E182 (≠ D201), E194 (= E213), D196 (≠ N215), F197 (≠ L216), K198 (≠ R217), Y199 (≠ H218), N245 (≠ H264), S247 (= S266), R314 (= R333), R319 (= R338), L326 (≠ E345), S327 (≠ A346), T328 (≠ R347), R329 (= R348), E331 (= E350)
binding l-methionine-s-sulfoximine phosphate: E130 (= E141), E132 (= E143), E187 (= E206), E194 (= E213), N238 (≠ P257), G239 (= G258), G241 (≠ A260), H243 (= H262), R296 (= R315), Y301 (≠ Q320), E302 (≠ A321), R314 (= R333), R333 (= R352)

4s0rD Structure of gs-tnra complex (see paper)
29% identity, 93% coverage: 28:449/452 of query aligns to 28:444/447 of 4s0rD

query
sites
4s0rD

I

F

S

T

D

D

L

I

P

L

G

G

I

T

A

I

R

K

G

N

K

V

A

E

V

I

P

P

A

V

S

S

K

Q

F

L

A

G

K

K

Q

A

D

-

Y

-

F

-

H

-

L

L

P

D

D

N

S

K

I

V

F

M

Y

F

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E
x
D

A

G

A

S

D

S

D

I

D

E

G

G

W
x
F

I
x
V

-
x
R

-
x
I

-

E

E

E

K

S

D

D

M

M

I

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

A

F

T

V

A

I

A

F

P

P

W

W

T

T

G

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

V

F

I

I

H

C

D

D

A

I

Y

Y

D

N

R

P

D

D

H

G

K

T

P

P

I

F

P

E

F

G

S

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

V

L

Q

K

L

E

Y

M

H

E

D

D

K

L

G

G

W

F

Q

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

L

L

V

F

A

K

R

L

N

D

I

-

D

-

P

-

A

-

R

E

E

K

I

G

E

E

P

P

M

T

M

L

G

E

R

L

S

N

G

D

R

K

P

G

A

G

A

Y

A

-

R

-

Q

-

A

-

Y

F

S
x
D

M

L

T

A

A

P

V

T

D

D

E

L

F

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

F

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G
x
A

A
x
P

G

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

G

P

A

V

V

K

R

L

S

A

C

D

D

E

D

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

D
x
G

E
x
V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H

N

H

L

S

S

I

L

I

-

D

-

M

F

E

K

S

N

G

G

D

V

N

N

I

A

F

F

-

F

-

D

-

E

-

N

S

A

G

D

P

L

Q

Q

G

L

G

S

E

E

T

T

D

A

A

K

F

-

Y

-

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

N

K

H

H

L

A

P

T

A

S

G

F

L

T

A

A

V

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

K

P

E
x
G

Q
x
Y

A
x
E

A
|
A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

I

I

R

R

V

I

P

P

L

A

S

S

G

R

P

G

E

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R
|
R

I

S

A

V

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

S

L

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

L

I

V

K

N

N

E

K

E

L

R

E

P

A

R

P

K

A

Q

P

F

I

K

D

G

R

D
x
N

A

I

Y
|
Y

A

V

-
x
M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

G

V

D

D

I

L

P

P

Q

A

V

T

M

L

G

A

Q

E

A

A

L

L

D

E

L

E

F

F

E
x
K

E
x
S

A

N

S

E

A

V

L

M

H

V

E

K

V

A

L

L

G

G

P

E

E

H

F

L

A

F

R

E

V
x
H

Y

F

S

I

I

E

V

A

K

K

R

E

A
x
I

E

E

Y

W

D

D

E
x
M

F

F

L

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H
x
Q

L

Y

L

M

active site: D56 (≠ E68), E135 (= E141), E137 (= E143), E192 (= E206), E199 (= E213), H248 (= H262), R319 (= R333), E336 (= E350), R338 (= R352)
binding glutamine: E137 (= E143), E192 (= E206), E199 (= E213), G244 (= G258), G246 (≠ A260), R301 (= R315), Y306 (≠ Q320), E307 (≠ A321), A308 (= A322)
binding magnesium ion: I66 (vs. gap), E135 (= E141), E135 (= E141), E199 (= E213), H248 (= H262), H248 (= H262), E336 (= E350), K403 (≠ E408), S404 (≠ E409), H419 (≠ V424)
binding : F63 (≠ W75), V64 (≠ I76), R65 (vs. gap), I66 (vs. gap), D161 (≠ S175), V193 (≠ G207), A194 (≠ G208), P195 (≠ A209), G241 (≠ D255), V242 (≠ E256), N243 (≠ P257), G305 (≠ E319), Y306 (≠ Q320), R319 (= R333), N374 (≠ D388), Y376 (= Y390), M378 (vs. gap), I426 (≠ A431), M430 (≠ E435), Q442 (≠ H447)

Sites not aligning to the query:

binding : 446

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
29% identity, 93% coverage: 28:449/452 of query aligns to 24:440/443 of 4lnkA

query
sites
4lnkA

I

F

S

T

D

D

L

I

P

L

G

G

I

T

A

I

R

K

G

N

K

V

A

E

V

I

P

P

A

V

S

S

K

Q

F

L

A

G

K
|
K

Q

A

D

-

Y

-

F

-

H

-

L

L

P

D

D

N

S

K

I

V

F
x
M

Y

F

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E
x
D

A

G

A

S

D

S

D

I

D

E

G

G

W

F

I

V

-

R

-

I

-

E

E

E

K

S

D

D

M

M

I

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

A

F

T

V

A

I

A

F

P

P

W

W

T

T

G

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

V

F

I

I

H

C

D

D

A

I

Y

Y

D

N

R

P

D

D

H

G

K

T

P

P

I

F

P

E

F

G

S

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

V

L

Q

K

L

E

Y

M

H

E

D

D

K

L

G

G

W

F

Q

S

P

D

V

F

-
x
N

V

L

A
x
G

P

P

E
|
E

M

P

E
|
E

F

F

L

L

V

F

A

K

R

L

N

D

I

-

D

-

P

-

A

-

R

E

E

K

I

G

E

E

P

P

M

T

M

L

G

E

R

L

S

N

G

D

R

K

P

G

A

G

A
x
Y

A

-

R

-

Q

-

A

-

Y

F

S

D

M

L

T

A

A

P

V

T

D

D

E

L

F

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

F

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G

A

A

P

G

G

Q

Q

L

H

E
|
E

I

I

N

D

L
x
F

R
x
K

H
x
Y

G

A

D

G

P

A

V

V

K

R

L

S

A

C

D

D

E

D

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

D

G

E

V

P
x
N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

H
x
L

S
|
S

I

L

I

-

D

-

M
x
F

E

K

S

N

G

G

D

V

N

N

I

A

F

F

-

F

-

D

-

E

-

N

S

A

G

D

P

L

Q

Q

G

L

G

S

E

E

T

T

D

A

A

K

F

-

Y

-

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

N

K

H

H

L

A

P

T

A

S

G

F

L

T

A

A

V

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

K

P

E

G

Q

Y

A
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

I

I

R

R

V

I

P

P

L

A

S
|
S

G

R

P

G

E
x
I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

I

S

A

V

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

S

L

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

L

I

V

K

N

N

E

K

E

L

R

E

P

A

R

P

K

A

Q

P

F

I

K

D

G

R

D

N

A

I

Y

Y

A

V

-

M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

G

V

D

D

I

L

P

P

Q

A

V

T

M

L

G

A

Q

E

A

A

L

L

D

E

L

E

F

F

E

K

E

S

A

N

S

E

A

V

L

M

H

V

E

K

V

A

L

L

G

G

P

E

E

H

F

L

A

F

R

E

V

H

Y

F

S

I

I

E

V

A

K

K

R

E

A

I

E

E

Y

W

D

D

E

M

F

F

L

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ E68), E131 (= E141), E133 (= E143), E188 (= E206), E195 (= E213), H244 (= H262), R315 (= R333), E332 (= E350), R334 (= R352)
binding adenosine-5'-diphosphate: K43 (= K47), M50 (≠ F58), N127 (vs. gap), G129 (≠ A139), E131 (= E141), F198 (≠ L216), K199 (≠ R217), Y200 (≠ H218), N246 (≠ H264), L247 (≠ H265), S248 (= S266), F250 (≠ M270), S324 (= S342), I327 (≠ E345), S328 (≠ A346), R330 (= R348)
binding glutamic acid: E133 (= E143), Y155 (≠ A169), E188 (= E206), V189 (≠ G207), N239 (≠ P257), G240 (= G258), G242 (≠ A260), H244 (= H262), R297 (= R315), E303 (≠ A321), R334 (= R352)
binding magnesium ion: E131 (= E141), E188 (= E206), E195 (= E213), H244 (= H262), E332 (= E350)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
29% identity, 93% coverage: 28:449/452 of query aligns to 24:440/443 of 4lniA

query
sites
4lniA

I

F

S

T

D

D

L

I

P

L

G

G

I

T

A

I

R

K

G

N

K

V

A

E

V

I

P

P

A

V

S

S

K

Q

F

L

A

G

K

K

Q

A

D

-

Y

-

F

-

H

-

L

L

P

D

D

N

S

K

I

V

F

M

Y

F

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E
x
D

A

G

A

S

D

S

D

I

D

E

G

G

W

F

I

V

-

R

-

I

-

E

E

E

K

S

D

D

M

M

I

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

A

F

T

V

A

I

A

F

P

P

W

W

T

T

G

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

V

F

I

I

H

C

D

D

A

I

Y

Y

D

N

R

P

D

D

H

G

K

T

P

P

I

F

P

E

F

G

S

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

V

L

Q

K

L

E

Y

M

H

E

D

D

K

L

G

G

W

F

Q

S

P

D

V

F

-
x
N

V

L

A
x
G

P

P

E
|
E

M

P

E
|
E

F

F

L

L

V

F

A

K

R

L

N

D

I

-

D

-

P

-

A

-

R

E

E

K

I

G

E

E

P

P

M

T

M

L

G

E

R

L

S

N

G

D

R

K

P

G

A

G

A
x
Y

A

-

R

-

Q

-

A

-

Y

F

S

D

M

L

T

A

A

P

V

T

D

D

E

L

F

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

F

F

E

E

I

I

D
x
E

G

A

I

S

T

H

Q

H

E
|
E

G

V

G

A

A

P

G

G

Q

Q

L

H

E
|
E

I

I

N
x
D

L
x
F

R
x
K

H
x
Y

G

A

D

G

P

A

V

V

K

R

L

S

A

C

D

D

E

D

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

D

G

E

V

P

N

G
|
G

S

S

A
x
G

M

M

H
|
H

I

C

H
x
N

H

L

S
|
S

I

L

I

-

D

-

M

F

E

K

S

N

G

G

D

V

N

N

I

A

F

F

-

F

-

D

-

E

-

N

S

A

G

D

P

L

Q

Q

G

L

G

S

E

E

T

T

D

A

A

K

F

-

Y

-

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

N

K

H

H

L

A

P

T

A

S

G

F

L

T

A

A

V

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

K

P

E

G

Q
x
Y

A
x
E

A
|
A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

Q

N

N

R
|
R

T

S

T

P

G

L

I

I

R
|
R

V

I

P

P

L

A

S

S

G

R

P

G

E
x
I

A
x
S

R

T

R
|
R

V

V

E
|
E

N

V

R
|
R

I

S

A

V

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

S

L

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

L

I

V

K

N

N

E

K

E

L

R

E

P

A

R

P

K

A

Q

P

F

I

K

D

G

R

D

N

A

I

Y

Y

A

V

-

M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

G

V

D

D

I

L

P

P

Q

A

V

T

M

L

G

A

Q

E

A

A

L

L

D

E

L

E

F

F

E

K

E

S

A

N

S

E

A

V

L

M

H

V

E

K

V

A

L

L

G

G

P

E

E

H

F

L

A

F

R

E

V

H

Y

F

S

I

I

E

V

A

K

K

R

E

A

I

E

E

Y

W

D

D

E

M

F

F

L

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

active site: D52 (≠ E68), E131 (= E141), E133 (= E143), E188 (= E206), E195 (= E213), H244 (= H262), R315 (= R333), E332 (= E350), R334 (= R352)
binding adenosine-5'-diphosphate: N127 (vs. gap), G129 (≠ A139), E131 (= E141), E183 (≠ D201), D197 (≠ N215), F198 (≠ L216), K199 (≠ R217), Y200 (≠ H218), N246 (≠ H264), S248 (= S266), R315 (= R333), R320 (= R338), I327 (≠ E345), S328 (≠ A346), R330 (= R348), E332 (= E350)
binding magnesium ion: E131 (= E141), E131 (= E141), E133 (= E143), E188 (= E206), E195 (= E213), E195 (= E213), H244 (= H262), E332 (= E350)
binding l-methionine-s-sulfoximine phosphate: E131 (= E141), E133 (= E143), Y155 (≠ A169), E188 (= E206), E195 (= E213), G240 (= G258), G242 (≠ A260), H244 (= H262), R297 (= R315), Y302 (≠ Q320), E303 (≠ A321), A304 (= A322), R315 (= R333), R334 (= R352)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
29% identity, 93% coverage: 28:449/452 of query aligns to 25:441/444 of P12425

query
sites
P12425

I

F

S

T

D

D

L

I

P

L

G

G

I

T

A

I

R

K

G

N

K

V

A

E

V

I

P

P

A

V

S

S

K

Q

F

L

A

G

K

K

Q

A

D

-

Y

-

F

-

H

-

L

L

P

D

D

N

S

K

I

V

F

M

Y

F

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E

D

A

G

A

S

D

S

D

I

D

E

G
|
G

W

F

I

V

-
x
R

-

I

-

E

E

E

K

S

D

D

M

M

I

Y

L

L

K

Y

P

P

D

D

M

L

S

N

T

T

A

F

T

V

A

I

A

F

P

P

W

W

T

T

G

A

D

E

W

K

-

G

-

K

T

V

L

A

Q

R

V

F

I

I

H

C

D

D

A

I

Y

Y

D

N

R

P

D

D

H

G

K

T

P

P

I

F

P

E

F

G

S

D

P

P

R

R

N

N

V

N

L

L

K

K

R

R

V

I

V

L

Q

K

L

E

Y

M

H

E

D

D

K

L

G

G

W

F

Q

S

P

D

V

F

-

N

V

L

A

G

P

P

E
|
E

M

P

E
|
E

F

F

L

L

V

F

A

K

R

L

N

D

I

-

D

-

P

-

A

-

R

E

E

K

I

G

E

E

P

P

M

T

M

L

G

E

R

L

S

N

G

D

R

K

P

G

A

G

A

Y

A

-

R

-

Q

-

A

-

Y

F

S

D

M

L

T

A

A

P

V

T

D

D

E

L

F

G

G

E

P

N

V

C

I

R

D

R

D

D

I

I

Y

V

D

L

F

E

A

L

E

E

A

E

Q

M

G

G

F

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G

A

A

P

G

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

G

P

A

V

V

K

R

L

S

A

C

D

D

E

D

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

K

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

D

G

E

V

P

N

G
|
G

S

S

A

G

M

M

H
|
H

I

C

H

N

H

L

S

S

I

L

I

-

D

-

M

F

E

K

S

N

G

G

D

V

N

N

I

A

F

F

-

F

-

D

-

E

-

N

S

A

G

D

P

L

Q

Q

G

L

G

S

E

E

T

T

D

A

A

K

F

-

Y

-

H

H

F

F

I

I

G

A

G

G

L

I

Q

V

N

K

H

H

L

A

P

T

A

S

G

F

L

T

A

A

V

V

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R

R

Y

L

V

V

K

P

E
x
G

Q

Y

A
x
E

A

A

P
|
P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

Q

N

N

R

R

T

S

T

P

G

L

I

I

R

R

V

I

P

P

L

A

S

S

G

R

P

G

E

I

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

I

S

A

V

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

S

L

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

D

G

G

L

I

V

K

N

N

E

K

E

L

R

E

P

A

R

P

K

A

Q

P

F

I

K

D

G

R

D

N

A

I

Y

Y

A

V

-

M

-

S

-

K

-

E

-

R

-

M

-

E

-

N

G

G

D

I

G

V

D

D

I

L

P

P

Q

A

V

T

M

L

G

A

Q

E

A

A

L

L

D

E

L

E

F

F

E

K

E

S

A

N

S

E

A

V

L

M

H

V

E

K

V

A

L

L

G

G

P

E

E

H

F

L

A

F

R

E

V

H

Y

F

S

I

I

E

V

A

K

K

R

E

A

I

E
|
E

Y

W

D

D

E

M

F

F

L

R

Q

T

V

Q

I

V

S

H

P

P

W

W

E

E

R

R

E

E

H

Q

L

Y

L

M

G59 (= G74) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (vs. gap) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E141) binding
E134 (= E143) binding
E189 (= E206) binding
V190 (≠ G207) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E213) binding
G241 (= G258) binding
H245 (= H262) binding
G302 (≠ E319) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ A321) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P323) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E350) binding
E424 (= E432) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

5dm3C Crystal structure of glutamine synthetase from chromohalobacter salexigens dsm 3043(csal_0679, target efi-550015) with bound adp
29% identity, 96% coverage: 13:444/452 of query aligns to 6:390/396 of 5dm3C

query
sites
5dm3C

K

K

T

T

Y

R

L

V

E

E

G

S

R

G

R

E

L

I

D

D

E

T

V

V

E

L

C

V

I

C

I

I

S

V

D

D

L

M

P

Q

G

G

I

R

A

L

R

M

G

G

K

K

A

R

V

L

P

H

A

A

S

R

K

H

F

F

A

V

K

D

Q

H

D

G

Y

W

-

E

F
x
T

H
|
H

L

C

P

C

D

N

S

Y

I

L

F

L

Y

Y

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E

-

A

-

D

-

G

-

W

-

I

-

E

-

K

-

D

-

M

-

I

I

L

M

K

K

P

P

D

D

M

L

S

A

T

T

A

L

T

R

A

C

A

V

P

P

W

W

T

L

G

-

D

E

W

G

T

T

L

A

Q

M

V

V

I

L

H

C

D

D

A

L

Y

L

D

D

-

H

R

R

D

T

H

H

K

A

P

E

I

V

P

P

F

H

S

A

P

P

R

R

N

A

V

I

L

L

K

K

R

R

V

Q

V

L

Q

A

L

R

Y

L

H

E

D

A

K

M

G

G

W

L

Q

E

P

A

V
x
I

V

M

A
|
A

P

T

E
|
E

M

L

E
|
E

F

F

L

L

V

F

A

E

R

K

N

S

I

L

D

D

P

E

A

I

R

R

E

K

I

-

E

-

P

-

M

-

G

-

R

-

S

-

G

G

R

R

P

F

A

R

A

T

R

K

Q

E

A

E

Y

H

S

-

M

-

T

-

A

-

V

-

D

-

E

-

F

-

G

-

P

-

V

V

I

L

D

R

D

P

I

L

Y

R

D

N

F

H

A

L

E

H

A

A

Q

A

G

G

F

I

E

P

I

V

D
x
E

G

G

I

T

T

K

Q

G

E
|
E

G

A

G

G

A

A

G

G

Q

Q

L

E

E
|
E

I

L

N

N

L

I

R
|
R

H
x
C

G

A

D

K

P

A

V

L

K

D

L

T

A

A

D

D

E

Y

V

H

F

T

Y

I

F

A

K

K

R

H

L

A

I

T

R

K

E

E

A

I

A

A

L

W

R

Q

H

Q

D

G

C

R

F

A

A

V

T

T

F

F

M

L

A

S

K

K

P

W

I

H

A

H

D

A

E

H

P

A

G

G

S

S

A

S

M

S

H
|
H

I

I

H
|
H

H
x
Q

S
|
S

I

L

I

-

D

-

M
x
W

E

K

S

Q

G

G

D

L

N

P

I

A

F

F

S

H

G

D

P

E

Q

R

G

D

-

A

-

L

G

G

E

M

T

S

D

A

A

L

F

M

Y

K

H

H

F

Y

I

L

G

A

G

G

L

L

Q

L

N

K

H

Y

L

A

P

P

A

D

G

Y

L

T

A

Y

V

F

M

L

A

A

P

P

Y

Y

V

L

N

N

S

S

Y

Y

R

K

R

R

Y

F

V

-

K

-

E

-

Q

Q

A

F

A

A

P

P

I

T

N

R

L

T

E

V

W

W

A

S

R

V

D

D

N

N

R
|
R

T

T

T

A

G

G

I

F

R

R

V

L

P

C

L

A

S

E

G

G

P

T

E

R

A
|
A

R

V

R
|
R

V

I

E
|
E

N

C

R
|
R

I

I

A

G

G

G

M

S

D

D

C

L

N

N

P

P

Y

Y

L

L

G

A

I

M

A

A

L

G

S

Q

L

L

A

A

C

A

G

G

Y

I

L

K

G

G

L

I

V

-

N

-

E

-

R

-

P

-

R

-

K

E

Q

E

F

C

K

L

G

A

D

L

A

P

Y

P

A

P

G

A

D

S

G

G

D

L

I

I

P

P

Q

Q

V

N

M

L

G

R

Q

D

A

A

L

M

D

E

L

A

F

L

E

R

E

G

A

S

S

T

A

M

L

L

H

R

E

E

V

A

L

M

G

G

P

E

E

D

F

V

A

V

R

D

V

H

Y

Y

S

V

I

R

V

A

K

A

R

E

A

V

E

E

Y

L

D

E

E

D

F

F

L

Q

Q

R

V

V

I

V

S

S

P

D

W

Y

E

E

active site: E115 (= E141), E117 (= E143), E162 (= E206), E169 (= E213), H218 (= H262), R286 (= R333), E303 (= E350), R305 (= R352)
binding adenosine-5'-diphosphate: T46 (≠ F51), H47 (= H52), I111 (≠ V137), A113 (= A139), E157 (≠ D201), R173 (= R217), C174 (≠ H218), H220 (= H264), Q221 (≠ H265), S222 (= S266), W224 (≠ M270), A299 (= A346), R301 (= R348)

A0R083 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
31% identity, 83% coverage: 76:449/452 of query aligns to 63:444/446 of A0R083

query
sites
A0R083

I

F

E

E

K

S

D

D

M

T

I

V

L

A

K

R

P

P

D

D

M

P

S

S

T

T

A

F

T

Q

A

V

A

L

P

P

W

W

-

K

T

T

G

S

D

D

-

G

-

N

-

H

W

Y

T

S

L

A

Q

R

V

M

I

F

H

C

D

D

A

I

Y

T

D

M

R

P

D

D

H

G

K

S

P

P

I

S

P

W

F

A

S

D

P

S

R

R

N

H

V

V

L

L

K

R

R

R

V

Q

V

L

Q

A

L

K

Y

A

H

S

D

D

K

L

G

G

W

F

Q

T

P

C

V

Y

V

V

A

H

P

P

E

E

M

I

E

E

F

F

L

L

V

L

A

K

R

P

N

G

I

P

D

N

P

D

A

G

R

T

E

P

I

P

E

E

P

P

M

-

G

-

R

-

S

-

G

-

R

-

P

-

A

-

A

A

A

D

R

N

Q

G

A

G

Y

Y

S

F

M

D

T

Q

A

A

V

V

D

H

E

D

F

A

G

A

P

P

V

N

I

F

D

R

-

R

D

H

I

A

Y

I

D

E

F

A

A

L

E

E

A

Q

Q

M

G

G

F

I

E

S

I

V

D

E

G

F

I

S

T

H

Q

H

E

E

G

G

G

A

A

P

G

G

Q

Q

L

Q

E

E

I

I

N

D

L

L

R

R

H

Y

G

A

D

D

P

A

V

L

K

S

L

M

A

A

D

D

E

N

V

V

F

M

Y

T

F

F

K

R

R

Y

L

L

I

V

R

K

E

E

A

V

A

A

L

L

R

A

H

D

D

G

C

V

F

R

A

A

T

S

F

F

M

M

A

P

K

K

P

P

I

F

A

A

D

E

E

H

P

P

G

G

S

S

A

A

M

M

H

H

I

T

H

H

H

M

S

S

I

L

I

F

D

-

M

-

E

E

S

G

G

D

D

T

N

N

I

A

F

F

S

H

G

S

P

P

Q

D

G

D

-

P

-

L

G

Q

E

L

T

S

D

D

A

V

F

A

Y

K

H

S

F

F

I

I

G

A

G

G

L

I

Q

L

N

E

H

H

L

A

P

N

A

E

G

I

L

S

A

A

V

V

M

T

A

N

P

Q

Y

W

V

V

N

N

S

S

Y

Y

R

K

R

R

Y

L

V

V

K

H

E

G

Q

G

A

E

A

A

P

P

I

T

N

A

L

A

E

S

W

W

A

G

R

A

D

A

N

N

R

R

T

S

T

A

G

L

I

V

R

R

V

V

P

P

L

M

S

Y

G

T

P

P

-

H

-

K

-

V

E

S

A

S

R

R

V

V

E

E

N

V

R

R

I

S

A

P

G

D

M

S

D

A

C

C

N

N

P

P

Y

Y

L

L

G

T

I

F

A

A

L

V

S

L

L

L

A

A

C

A

G

G

Y

L

L

R

G

G

L

V

V

E

N
x
K

E

G

E

Y

R

V

P

L

R

G

K

P

Q

Q

F

A

K

E

G

D

D

N

A

V

Y

W

A

S

-

L

-

T

-

Q

-

E

-

R

-

A

-

M

G

G

D

Y

G

R

D

E

I

L

P

P

Q

T

V

S

M

L

G

G

Q

N

A

A

L

L

D

E

L

S

F

M

E

E

E

N

A

S

S

E

A

L

L

V

H

A

E

E

V

A

L

L

G

G

P

E

E

H

F

V

A

F

R

D

V

Y

Y

F

S

L

I

R

V

N

K

K

R

R

A

S

E

E

Y

W

D

E

E

N

F

Y

L

R

Q

S

V

H

I

V

S

T

P

P

W

Y

E

E

R

L

E

K

H

N

L

Y

L

L

K363 (≠ N377) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7tfaB Glutamine synthetase (see paper)
28% identity, 93% coverage: 28:449/452 of query aligns to 23:438/441 of 7tfaB

query
sites
7tfaB

I

F

S

T

D

D

L

L

P

L

G

G

I

T

A

I

R

K

G

N

K

V

A

E

V

I

P

P

A

V

S

S

K

Q

F

L

A

E

K

K

Q

A

D

-

Y

-

F

-

H

-

L

L

P

D

D

N

S

K

I

M

F

M

Y

F

Q

-

T

-

I

-

T

-

G

-

D

-

W

-

A

-

E

D

A

G

A

S

D

S

D

I

D

E

G

G

W
x
Y

I

V

-
x
R

-

I

-

E

E

E

K

S

D

D

M

M

I

Y

L

L

K

Y

P

P

D

D

M

L

S

D

T

T

A

W

T

V

A

V

A

F

P

P

W

W

-

V

T

T

G

S

D

D

W

R

T

V

L

A

Q

R

V

L

I

I

H

C

D

D

A

I

Y

Y

D

K

R

P

D

D

H

G

K

S

P

P

I

F

P

A

F

G

S

D

P

P

R

R

N

G

V

I

L

L

K

K

R

R

V

V

V

L

Q

K

L

E

Y

A

H

E

D

E

K

L

G

G

W

Y

Q

T

P

S

V

M

-

N

V

V

A

G

P

P

E
|
E

M

P

E
|
E

F

F

L

L

V

F

A

K

R

T

N

D

I

E

-

K

-

G

D

D

P

P

A

T

R

T

E

E

I

L

E

N

P

D

M

Q

M

G

G

G

R

-

S

-

G

-

R

-

P

-

A

-

R

-

Q

-

A
x
Y

Y

F

S

D

M

L

T

A

A

P

V

M

D

D

E

L

F

G

G

E

P

N

V

C

I

R

D

R

D

E

I

I

Y

V

D

L

F

K

A

L

E

E

A

E

Q

M

G

G

F

F

E

E

I

I

D

E

G

A

I

S

T

H

Q

H

E
|
E

G
x
V

G

A

A

P

G

G

Q

Q

L

H

E
|
E

I

I

N

D

L

F

R

K

H

Y

G

A

D

D

P

A

V

V

K

K

L

A

A

A

D

D

E

Q

V

I

F

Q

Y

T

F

F

K

K

R

L

L

V

I

V

R

K

E

T

A

I

A

A

L

R

R

Q

H

H

D

G

C

L

F

H

A

A

T

T

F

F

M

M

A

P

K

K

P

P

I

L

A

F

D
x
G

E

V

P
x
N

G
|
G

S
|
S

A
x
G

M

M

H
|
H

I

C

H

N

H

Q

S

S

I

L

I

F

D

-

M

-

E

K

S

D

G

N

D

E

N

N

I

V

F

F

-

Y

-

D

S

E

G

T

P

D

Q

E

G

L

G

G

E

L

T

S

D

Q

A

T

F

A

Y

R

H

H

F

Y

I

M

G

A

G

G

L

I

Q

L

N

K

H

H

L

A

P

R

A

A

G

M

L

A

A

A

V

I

M

T

A

N

P

P

Y

T

V

V

N

N

S

S

Y

Y

R

K

R
|
R

Y

L

V

V

K

P

E
x
G

Q
x
Y

A
x
E

A

A

P

P

I

C

N

Y

L

V

E

A

W

W

A

S

R

A

D

S

N

N

R
|
R

T

S

T

P

G

M

I

I

R

R

V

I

P

P

L

A

S

S

G

R

P

G

E

L

A

S

R

T

R

R

V

V

E
|
E

N

V

R

R

I

N

A

P

G

D

M

P

D

A

C

A

N

N

P

P

Y

Y

L

L

G

A

I

L

A

A

L

V

S

M

L

L

A

R

C

A

G

G

Y

L

L

D

G

G

L

I

V

K

N

R

E

Q

E

M

R

A

P

L

R

P

K

A

Q

P

F

I

K

D

G

R

D

N

A

I

Y

Y

A

V

-

M

-

S

-

E

-

R

-

I

-

E

G

G

D

I

G

P

D

S

I

L

P

P

Q

A

V

D

M

L

G

K

Q

E

A

A

L

L

D

S

L

E

F

L

E

I

E

R

A

S

S

E

A

V

L

I

H

S

E

D

V

A

L

L

G

G

P

D

E

H

F

A

A

L

R

A

V

Y

Y

F

S

Y

I

E

V

L

K

K

R

E

A
x
I

E

E

Y

W

D

D

E
x
M

F

Y

L

R

Q

T

V

Q

I

V

S

H

P

Q

W
|
W

E

E

R

R

E

D

H
x
Q

L

Y

L

L

binding glutamine: E131 (= E143), Y153 (≠ A173), E186 (= E206), N237 (≠ P257), G238 (= G258), S239 (=