SitesBLAST – Find functional sites

 

SitesBLAST

Comparing GFF1139 FitnessBrowser__Marino:GFF1139 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

Q01468 2-hydroxymuconate tautomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
71% identity, 100% coverage: 1:63/63 of query aligns to 1:63/63 of Q01468

query
sites
Q01468
M
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M
P
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P
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I
A
 
A
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Q
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I
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H
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I
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L
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E
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G
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R
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S
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D
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E
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Q
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L
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E
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L
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D
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A
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P
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L
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R
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V
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I
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E
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M
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A
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K
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G
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H
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F
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G
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I
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G
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G
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E
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L
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A
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K
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V
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R
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R

5tigA Crystal structure of 4-oxalocrotonate tautomerase inactivated by brhpd (see paper)
71% identity, 98% coverage: 2:63/63 of query aligns to 1:62/62 of 5tigA

query
sites
5tigA
P
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P
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I
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A
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Q
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I
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H
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I
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L
E
 
E
G
 
G
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R
S
 
S
D
 
D
E
 
E
Q
 
Q
K
 
K
E
 
E
M
 
T
L
 
L
I
 
I
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R
E
 
E
V
 
V
T
 
S
D
 
E
A
 
A
I
 
I
S
 
S
R
 
R
S
 
S
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L
G
 
D
A
 
A
P
 
P
V
 
L
E
 
T
S
 
S
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R
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V
I
 
I
I
 
I
T
 
T
E
 
E
M
 
M
P
 
A
K
 
K
Q
 
G
H
 
H
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F
G
 
G
I
 
I
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G
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G
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E
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L
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A
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S
M
 
K
L
 
V
G
x
R
R
 
R

1bjpA Crystal structure of 4-oxalocrotonate tautomerase inactivated by 2- oxo-3-pentynoate at 2.4 angstroms resolution (see paper)
71% identity, 98% coverage: 2:63/63 of query aligns to 1:62/62 of 1bjpA

query
sites
1bjpA
P
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P
V
x
I
A
 
A
Q
 
Q
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I
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H
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I
L
 
L
E
 
E
G
 
G
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R
S
 
S
D
 
D
E
 
E
Q
 
Q
K
 
K
E
 
E
M
 
T
L
 
L
I
 
I
R
 
R
E
 
E
V
 
V
T
 
S
D
 
E
A
 
A
I
 
I
S
 
S
R
 
R
S
 
S
L
 
L
G
 
D
A
 
A
P
 
P
V
 
L
E
 
T
S
 
S
I
 
V
R
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R
I
 
V
I
 
I
I
 
I
T
 
T
E
 
E
M
 
M
P
 
A
K
 
K
Q
 
G
H
 
H
F
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F
G
 
G
I
 
I
G
 
G
G
 
G
Q
 
E
S
 
L
A
 
A
K
 
S
M
 
K
L
 
V
G
x
R
R
 
R

6bgnA Crystal structure of 4-oxalocrotonate tautomerase after incubation with 5-fluoro-2-hydroxy-2,4-pentadienoate (see paper)
75% identity, 90% coverage: 2:58/63 of query aligns to 1:57/59 of 6bgnA

query
sites
6bgnA
P
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P
V
 
I
A
 
A
Q
 
Q
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I
N
 
H
I
 
I
L
 
L
E
 
E
G
 
G
R
 
R
S
 
S
D
 
D
E
 
E
Q
 
Q
K
 
K
E
 
E
M
 
T
L
 
L
I
 
I
R
 
R
E
 
E
V
 
V
T
 
S
D
 
E
A
 
A
I
 
I
S
 
S
R
 
R
S
 
S
L
 
L
G
 
D
A
 
A
P
 
P
V
 
L
E
 
T
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S
I
 
V
R
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R
I
 
V
I
 
I
I
 
I
T
 
T
E
 
E
M
 
M
P
 
A
K
 
K
Q
 
G
H
 
H
F
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F
G
 
G
I
 
I
G
 
G
G
 
G
Q
 
E
S
 
L
A
 
A

6ghwC Substituting the prolines of 4-oxalocrotonate tautomerase with non- canonical analogue (2s)-3,4-dehydroproline (see paper)
75% identity, 90% coverage: 2:58/63 of query aligns to 1:57/57 of 6ghwC

query
sites
6ghwC
P
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P
V
 
I
A
 
A
Q
 
Q
I
 
I
N
 
H
I
 
I
L
 
L
E
 
E
G
 
G
R
 
R
S
 
S
D
 
D
E
 
E
Q
 
Q
K
 
K
E
 
E
M
 
T
L
 
L
I
 
I
R
 
R
E
 
E
V
 
V
T
 
S
D
 
E
A
 
A
I
 
I
S
 
S
R
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R
S
 
S
L
 
L
G
x
D
A
 
A
P
 
P
V
 
L
E
 
T
S
 
S
I
 
V
R
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R
I
 
V
I
 
I
I
 
I
T
 
T
E
 
E
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M
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A
K
 
K
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G
H
 
H
F
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F
G
 
G
I
 
I
G
 
G
G
 
G
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E
S
 
L
A
 
A

5cloM Crystal structure of a 4-oxalocrotonate tautomerase mutant in complex with nitrostyrene at 2.3 angstrom (see paper)
72% identity, 90% coverage: 2:58/63 of query aligns to 1:57/57 of 5cloM

query
sites
5cloM
P
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P
V
 
I
A
 
A
Q
 
Q
I
 
I
N
 
H
I
 
I
L
 
L
E
 
E
G
 
G
R
 
R
S
 
S
D
 
D
E
 
E
Q
 
Q
K
 
K
E
 
E
M
 
T
L
 
L
I
 
I
R
 
R
E
 
E
V
 
V
T
 
S
D
 
E
A
 
A
I
 
I
S
 
S
R
 
R
S
 
S
L
 
L
G
 
D
A
 
A
P
 
P
V
 
L
E
 
T
S
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S
I
 
V
R
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R
I
 
V
I
 
I
I
 
I
T
 
T
E
 
E
M
 
Y
P
 
A
K
 
K
Q
 
G
H
 
H
F
x
A
G
 
G
I
 
I
G
 
G
G
 
G
Q
 
E
S
 
L
A
 
A

P70994 2-hydroxymuconate tautomerase; (2Z,4E)-2-hydroxyhexa-2,4-dienedioate keto-enol isomerase; 4-oxalocrotonate tautomerase; 4-OT; EC 5.3.2.6 from Bacillus subtilis (strain 168) (see paper)
43% identity, 89% coverage: 1:56/63 of query aligns to 1:56/62 of P70994

query
sites
P70994
M
 
M
P
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P
V
 
Y
A
 
V
Q
 
T
I
 
V
N
 
K
I
 
M
L
 
L
E
 
E
G
 
G
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R
S
 
T
D
 
D
E
 
E
Q
 
Q
K
 
K
E
 
R
M
 
N
L
 
L
I
 
V
R
 
E
E
 
K
V
 
V
T
 
T
D
 
E
A
 
A
I
 
V
S
 
K
R
 
E
S
 
T
L
 
T
G
 
G
A
 
A
P
 
S
V
 
E
E
 
E
S
 
K
I
 
I
R
 
V
I
 
V
I
 
F
I
 
I
T
 
E
E
 
E
M
 
M
P
 
R
K
 
K
Q
 
D
H
 
H
F
 
Y
G
 
A
I
 
V
G
 
A
G
 
G
Q
 
K

2opaA Ywhb binary complex with 2-fluoro-p-hydroxycinnamate
42% identity, 87% coverage: 2:56/63 of query aligns to 1:55/61 of 2opaA

query
sites
2opaA
P
 
P
V
 
Y
A
 
V
Q
 
T
I
 
V
N
 
K
I
x
M
L
 
L
E
 
E
G
 
G
R
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R
S
 
T
D
 
D
E
 
E
Q
 
Q
K
 
K
E
 
R
M
 
N
L
 
L
I
 
V
R
 
E
E
 
K
V
 
V
T
 
T
D
 
E
A
 
A
I
 
V
S
 
K
R
 
E
S
 
T
L
 
T
G
 
G
A
 
A
P
 
S
V
 
E
E
 
E
S
 
K
I
 
I
R
x
V
I
 
V
I
 
F
I
 
I
T
 
E
E
 
E
M
 
M
P
 
R
K
 
K
Q
 
D
H
 
H
F
x
Y
G
 
A
I
 
V
G
 
A
G
 
G
Q
 
K

Sites not aligning to the query:

Query Sequence

>GFF1139 FitnessBrowser__Marino:GFF1139
MPVAQINILEGRSDEQKEMLIREVTDAISRSLGAPVESIRIIITEMPKQHFGIGGQSAKM
LGR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory