SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1184 FitnessBrowser__Phaeo:GFF1184 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5SKN9 Long-chain-fatty-acid--CoA ligase; Long-chain fatty acyl-CoA synthetase; LC-FACS; EC 6.2.1.3 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
35% identity, 95% coverage: 26:540/542 of query aligns to 24:535/541 of Q5SKN9

query
sites
Q5SKN9

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T184 (= T192) binding
G302 (≠ A308) binding
Q322 (= Q327) binding
G323 (≠ V328) binding
T327 (= T332) binding
E328 (= E333) binding
D418 (= D424) binding
K435 (= K441) binding
K439 (≠ I445) binding

1v26B Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
34% identity, 95% coverage: 26:540/542 of query aligns to 17:504/510 of 1v26B

query
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1v26B

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active site: T177 (= T192), H197 (≠ M212), H223 (= H236), T320 (= T332), E321 (= E333), K432 (≠ I445), W437 (≠ N450)
binding adenosine monophosphate: G295 (≠ A308), S296 (≠ P309), A297 (≠ P310), Q315 (= Q327), G316 (≠ V328), Y317 (= Y329), G318 (= G330), L319 (= L331), T320 (= T332), D411 (= D424), I423 (= I436), K428 (= K441), K432 (≠ I445), W437 (≠ N450)
binding magnesium ion: T177 (= T192), E321 (= E333)

1v25A Crystal structure of tt0168 from thermus thermophilus hb8 (see paper)
35% identity, 85% coverage: 26:487/542 of query aligns to 17:464/491 of 1v25A

query
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1v25A

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active site: T177 (= T192), H197 (≠ M212), H223 (= H236), T320 (= T332), E321 (= E333), K432 (≠ I445), W437 (≠ N450)
binding phosphoaminophosphonic acid-adenylate ester: F222 (= F235), H223 (= H236), V224 (≠ C237), W227 (= W240), G295 (≠ A308), S296 (≠ P309), A297 (≠ P310), Q315 (= Q327), G316 (≠ V328), Y317 (= Y329), G318 (= G330), L319 (= L331), T320 (= T332), D411 (= D424), I423 (= I436), R426 (= R439), K428 (= K441), K432 (≠ I445), W437 (≠ N450)
binding magnesium ion: T177 (= T192), E321 (= E333)

P9WQ37 Long-chain-fatty-acid--CoA ligase FadD13; Fatty acyl-CoA ligase; FACL; FACL13; Fatty acyl-CoA synthetase; ACS; FACS; Very-long-chain fatty-acyl-CoA synthetase; ACSVL; EC 6.2.1.3 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 4 papers)
32% identity, 92% coverage: 45:541/542 of query aligns to 29:499/503 of P9WQ37

query
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P9WQ37

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K172 (= K200) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Slight increase of susceptibility to proteolysis.
R195 (≠ K227) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.
R197 (vs. gap) mutation to A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-195 and A-244.
V209 (≠ C237) mutation to D: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced. Slight increase of susceptibility to proteolysis.
A211 (≠ G239) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.
T214 (≠ H242) mutation to W: Shows a marked decrease in the activity with lauric and palmitic acid (C12 and C16 fatty acid) with a simultaneous increase in the activity with caprylic acid (C8 fatty acid).
R244 (≠ G272) mutation to A: Alteration of the strength of the membrane binding; when associated with A-17; A-195; A-195 and A-197.
A302 (≠ G330) mutation to G: Slight increase of the fatty acyl-CoA ligase activity. Reduced rate of proteolytic degradation.; mutation to W: Does not show activity with small, medium or long acyl chains.
W377 (≠ Y419) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding. No significant change in the total expression level, however the cytoplasmic expression is low. Slight increase of susceptibility to proteolysis.
D382 (= D424) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. No significant change in the total expression level, however the cytoplasmic expression is reduced.
R397 (= R439) mutation to A: Reduction of binding affinity for fatty acids.
S404 (= S446) mutation to A: Slight reduction of the fatty acyl-CoA ligase activity. Enhanced affinity towards palmitic acid binding.
G406 (= G448) mutation to L: No effect on the formation of acyl-adenylate intermediate. However, it shows very poor catalytic efficiency to form acyl-CoA.
K487 (= K529) mutation to A: Strong reduction of the fatty acyl-CoA ligase activity. Reduction of binding affinity for ATP.

Sites not aligning to the query:

9 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-195; A-197 and A-244.
17 R→A: Alteration of the strength of the membrane binding; when associated with A-9; A-17; A-197 and A-244.

6ijbB Structure of 3-methylmercaptopropionate coa ligase mutant k523a in complex with amp and mmpa (see paper)
29% identity, 92% coverage: 45:540/542 of query aligns to 39:534/538 of 6ijbB

query
sites
6ijbB

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G

G

E

E

N
x
W

I

I

S

S

V

V

E

E

V

L

E

E

G

N

V

I

L

A

M

V

G

A

H

H

P

P

D

K

V

L

N

A

L

T

A

A

V

V

V

I

A

G

K

V

P

P

D

H

D

P

K

K

W

W

G

D

E

E

V

R

P

P

C

L

A

L

F

V

V

A

E

V

L

K

K

A

P

E

G

G

A

E

T

D

V

P

D

S

P

E

A

A

D

E

L

L

I

L

R

E

F

F

A

F

R

D

E

G

T

K

L

I

A

A

G

K

F

W

K

Q

A

V

P

P

K

D

Q

K

V

V

F

F

Q

V

E

D

-

A

L

L

P

P

K

L

T

N

S

A

T

T

G

G

K
x
A

I

V

Q

L

K

K

F

R

E

K

L

L

R

R

Q

D

Q

E

A

F

K

K

active site: T185 (= T192), H205 (≠ M212), H231 (= H236), S329 (≠ T332), E330 (= E333), K438 (≠ I445), W443 (≠ N450), A523 (≠ K529)
binding 3-(methylsulfanyl)propanoic acid: W235 (= W240), G303 (= G307), A325 (≠ V328), W326 (≠ Y329), G327 (= G330), M328 (≠ L331), P333 (vs. gap), L334 (≠ Y335)
binding adenosine monophosphate: T185 (= T192), H231 (= H236), G302 (≠ A306), G303 (= G307), A304 (= A308), A305 (≠ P309), H324 (≠ Q327), A325 (≠ V328), W326 (≠ Y329), G327 (= G330), M328 (≠ L331), S329 (≠ T332), Q359 (= Q363), D417 (= D424), I429 (= I436), R432 (= R439)

3r44A Mycobacterium tuberculosis fatty acyl coa synthetase (see paper)
32% identity, 92% coverage: 45:541/542 of query aligns to 32:499/502 of 3r44A

query
sites
3r44A

R

R

K

M

T

T

Y

Y

A

A

A

Q

Y

M

Y

N

D

A

R

L

C

A

T

N

R

R

L

C

A

A

S

D

A

V

L

L

A

T

G

A

M

L

G

G

V

I

R

A

P

K

G

G

E

D

V

R

V

V

A

A

T

L

L

L

I

M

P

P

N

N

L

S

P

V

A

E

Q

F

A

C

E

C

A

L

H

F

F

Y

G

G

V

A

P

A

A

K

C

L

G

G

A

A

V

V

L

A

N

V

T

P

I

I

N

N

T

T

R

R

L

L

D

A

V

A

S

P

T

E

V

V

A

S

Y

F

I

I

F

L

D

S

H

D

G

S

E

G

A

S

K

K

V

V

L

I

V

Y

D

G

S

A

Q

P

F

S

L

A

T

P

L

V

A

I

E

D

E

A

A

I

K

R

A

A

A

Q

C

A

D

D

G

P

L

P

G

G

P

T

L

V

I

T

I

D

E

W

V

I

P

-

D

-

Q

-

A

-

S

-

Y

-

P

-

A

-

S

-

G

G

R

A

H

D

P

S

I

L

Y

A

E

E

D

R

I

L

L

R

A

S

A

A

H

D

D

E

F

-

D

-

W

-

I

-

M

-

P

P

Q

A

D

V

E

E

W

C

-

G

-

D

E

D

S

N

L

L

A

F

L

I

N

M

Y

Y

T
|
T

S

S

G

G

T

-

G

-

R

H

P

P

K

K

G

G

V

V

Y

H

H

T

H
|
H

R
x
E

G

S

A

V

Y
x
H

L

-

M

-

T

-

M

-

G

S

T
x
A

V

A

I

S

S

S

W

W

R

A

M

S

V

T

M

I

Q

D

P

V

K

R

Y

Y

-

R

-

D

-

R

-

L

L

L

A

L

I

P

V

L

P

P

L

M

F

F

H
|
H

C

V

N

A

G

A

W

L

N

T

H

T

T

V

W

I

M

F

M

S

P

A

V

M

L

R

G

G

G

V

T

T

L

L

I

I

C

S

C

M

R

P

D

Q

I

F

T

D

A

A

P

T

A

K

I

V

Y

W

G

S

A

L

I

I

A

V

D

E

E

E

G

R

A

V

T

C

H

I

F

G

G

G

G

A

A

V

P

P

I

A

V

I

L

L

N

N

M

F

L

M

V

R

N

Q

A

V

-

P

-

E

-

F

A

A

E

E

E

L

D

D

R

A

R

P

Q

D

F

F

D

R

H

Y

T

-

V

-

E

-

V

-

F

F

T

I

A

T

G

G

A

G

P

A

P

P

A

M

P

P

A

E

T

A

L

L

E

I

K

K

I

I

-

Y

E

A

A

A

L

K

G

N

F

I

H

E

V

V

T

V

Q

Q

V

G

Y

Y

G

A

L

L

T
|
T

E
|
E

T

S

Y

C

G

G

H

G

V

G

T

T

E

L

C

L

L

L

W

-

K

-

G

-

D

-

S

S

W

E

D

D

T

A

L

L

D

R

Q

K

Q

A

G

G

R

S

A

A

A

G

I

R

K

A

A

T

R

-

Q

-

G

-

V

-

A

-

F

-

P

-

M

M

M

F

D

T

H

D

I

V

T

A

V

V

M

R

R

G

D

D

M

G

Q

-

I

-

A

V

M

I

N

R

G

E

Q

H

D

G

Q

E

G

G

E

E

I

V

V

V

M

I

R

K

G

S

N

D

S

I

V

L

M

L

K

K

G

E

Y

Y

L

W

K

N

N

R

P

P

D

E

A

A

T

T

A

R

E

D

A

A

F

F

Q

D

G

N

G

G

Y

W

F

F

H

R

S

T

G

G

D

D

I

I

A

G

V

E

Q

I

H

D

P

D

D

E

G

G

Y

Y

I

L

Q

Y

I

I

A

K

D

D

R

R

A

L

K

K

D

D

I

M

I

I

I
|
I

S

S

G

G

E

E

N
|
N

I

V

S

Y

S

P

V

A

E

E

V

I

E

E

G

S

V

V

L

I

M

I

G

G

H

V

P

P

D

G

V

V

N

S

L

E

A

V

A

A

V

V

V

I

A

G

K

L

P

P

D

D

D

E

K

K

W

W

G

G

E

E

V

I

P

A

C

A

A

A

F

I

V

V

E

-

L

V

K

A

P

D

G

Q

A

N

T

E

V

V

D

S

P

E

A

Q

D

Q

L

I

I

V

R

E

F

Y

A

C

R

G

E

T

T

R

L

L

A

A

G

R

F

Y

K

K

A

L

P

P

K

K

Q

K

V

V

V

I

F

F

Q

A

E

E

-

A

L

I

P

P

K

R

T

N

S

P

T

T

G

G

K
|
K

I

I

Q

L

K

K

F

T

E

V

L

L

R

R

Q

E

Q

Q

A

Y

K

S

A

A

active site: T167 (= T192), A184 (≠ T216), H208 (= H236), T304 (= T332), E305 (= E333), I403 (= I445), N408 (= N450), K487 (= K529)
binding histidine: H178 (= H206), E179 (≠ R207), H182 (≠ Y210)

Sites not aligning to the query:

binding histidine: 4, 5, 6, 7

6ihkB Structure of mmpa coa ligase in complex with adp (see paper)
29% identity, 92% coverage: 45:540/542 of query aligns to 39:531/533 of 6ihkB

query
sites
6ihkB

R

R

K

T

T

N

Y

W

A

G

A

E

Y

I

Y

A

D

A

R

N

C

A

T

R

R

R

L

M

A

G

S

S

A

A

L

L

A

T

G

K

M

L

G

G

V

L

R

Q

P

P

G

Q

E

D

V

R

V

I

A

G

T

T

L

L

I

A

P

W

N

N

L

N

P

R

A

R

Q

H

A

L

E

E

A

I

H

Y

F

Y

G

A

V

A

P

S

A

G

C

A

G

G

A

F

V

V

L

C

N

H

T

T

I

I

N

N

T

P

R

R

L

L

D

F

V

P

S

E

T

Q

V

L

A

V

Y

Y

I

I

F

I

D

N

H

H

G

A

E

Q

A

D

K

R

V

V

V

L

L

F

V

F

D

D

S

A

Q

T

F

F

L

L

T

P

L

L

A

V

E

A

A

I

K

R

A

D

A

Q

C

L

D

T

G

E

L

V

G

K

P

H

L

F

I

V

I

L

E

M

V

G

P

P

D

R

D

N

Q

E

A

D

S

A

Y

L

P

Q

A

Q

S

I

G

P

R

G

H

L

P

E

I

F

Y

Y

E

D

D

E

I

L

L

I

A

E

A

T

A

G

A

D

H

T

D

D

F

F

D

E

W

W

I

P

M

V

P

-

Q

F

D

D

E

E

W

N

E

T

S

A

L

S

A

S

L

L

N

C

Y

Y

T
|
T

S

S

G

G

T

-

G

-

R

H

P

P

K

K

G

G

V

V

V

L

Y

Y

H

S

H

H

R

R

G

S

A

T

Y

V

L

L

M
x
H

T

S

M

F

G

A

T

S

-

N

-

T

-

R

-

D

V

V

I

I

S

G

W

Y

R

S

M

A

V

M

M

-

Q

-

P

D

K

V

Y

V

L

M

A

P

I

V

V

V

P

P

L

M

F

F

H
|
H

C

V

N

N

G

A

W

W

N

G

H

S

T

P

W

Y

M

G

M

C

P

A

V

M

L

S

G

G

G

A

T

Q

L

M

I

V

C

L

-

P

C

G

R

P

D

D

I

L

T

H

A

G

P

E

A

A

I

L

Y

V

G

N

A

L

I

I

A

D

D

T

E

Y

G

G

A

V

T

T

H

L

F

A

G

M

G

G

A

V

P

P

I

T

V

I

L

W

N

Q

M

G

L

L

V

L

N

A

A

H

A

A

E

A

E

K

D

C

R

G

R

T

Q

K

F

L

D

E

H

S

T

L

V

E

E

R

V

T

F

V

T

I

A
x
G

G
|
G

A
|
A

P
x
A

P

C

A

P

P

P

A

S

T

M

L

I

E

A

K

T

I

F

-

R

E

E

A

K

L

Y

G

G

F

V

H

D

V

T

T

V

Q
x
H

V
x
A

Y
x
W

G
|
G

L
x
M

T
x
S

E
|
E

T

M

-

S

-

P

Y

L

G

G

H

T

V

A

T

N

E

I

C

P

L

L

W

A

K

K

G

H

D

R

S

K

W

L

D

P

T

I

L

E

D

E

Q

Q

Q

H

G

-

R

-

A

-

A

K

I

L

K

R

A

E

R

N

Q
|
Q

G

G

V

R

A

P

F

-

P

P

M

F

M

G

D

V

H

E

I

L

T

K

V

I

M

V

R

D

D

D

M

G

Q

N

Q

D

I

L

A

P

M

H

N

D

G

G

Q

V

D

T

Q

Q

G

G

E

D

I

L

V

M

M

V

R

R

G

G

N

H

S

W

V

V

M

L

K

D

G

S

Y

Y

L

F

K

Q

N

L

P

K

D

D

A

Q

T

-

A

-

E

E

A

L

F

L

Q

Q

G

D

G

G

Y

W

F

F

H

A

S

T

G

G

D
|
D

I

V

A

A

V

T

Q

L

H

D

P

P

D

D

G

G

Y

Y

I

M

Q

T

I
|
I

A

R

D

D

R
|
R

A

S

K

K

D

D

I

I

I
x
K

S

S

G

G

E

E

N
x
W

I

I

S

S

V

V

E

E

V

L

E

E

G

N

V

I

L

A

M

V

G

A

H

H

P

P

D

K

V

L

N

A

L

T

A

A

V

V

V

I

A

G

K

V

P

P

D

H

D

P

K

K

W

W

G

D

E

E

V

R

P

P

C

L

A

L

F

V

V

A

E

V

L

K

K

A

P

E

G

G

A

E

T

D

V

P

D

S

P

E

A

A

D

E

L

L

I

L

R

E

F

F

A

F

R

D

E

G

T

K

L

I

A

A

G

K

F

W

K

Q

A

V

P

P

K

D

Q

K

V

V

F

F

Q

V

E

D

-

A

L

L

P

P

K

L

T

N

S

A

T

T

G

G

K
|
K

I

V

Q

L

K

K

F

R

E

K

L

L

R

R

Q

D

Q

E

A

F

K

K

active site: T185 (= T192), H202 (≠ M212), H228 (= H236), S326 (≠ T332), E327 (= E333), K435 (≠ I445), W440 (≠ N450), K520 (= K529)
binding adenosine-5'-diphosphate: T185 (= T192), H228 (= H236), G299 (≠ A306), G300 (= G307), A301 (= A308), A302 (≠ P309), H321 (≠ Q327), A322 (≠ V328), W323 (≠ Y329), G324 (= G330), M325 (≠ L331), S326 (≠ T332), Q356 (= Q363), D414 (= D424), I426 (= I436), R429 (= R439), K520 (= K529)

5x8fB Ternary complex structure of a double mutant i454ra456k of o- succinylbenzoate coa synthetase (mene) from bacillus subtilis bound with amp and its product analogue osb-ncoa at 1.76 angstrom (see paper)
28% identity, 95% coverage: 26:540/542 of query aligns to 8:481/485 of 5x8fB

query
sites
5x8fB

L

L

R

M

R

Q

A

R

A

A

H

Q

V

L

F

T

A

P

D

E

V

R

P

I

A

A

V

L

V

I

Y
|
Y

G
x
E

N

D

H

Q

R

T

K

V

T

T

Y

F

A

A

A

E

Y

L

Y

F

D

A

R

A

C

S

T

K

R

R

L

M

A

A

S

E

A

Q

L

L

A

A

G

A

M

H

G

S

V

V

R

R

P

K

G

G

E

D

V

T

V

A

A

A

T

I

L

L

I

L

P

Q

N

N

L

-

P

-

A

-

Q

R

A

A

E

E

A

M

H

V

F

Y

G

A

V

V

P
x
H

A

A

C

C

-

F

-

L

G

G

A

V

V

K

L

A

N

V

T

L

I

L

N

N

T

T

R
x
K

L

L

D

S

V

T

S

H

T

E

V

R

A

L

Y

F

I

Q

F

L

D

E

H

D

G

S

E

G

A

S

K

G

V

F

V

L

L

L

V

T

D

D

S

S

Q

S

F

F

L

E

T

K

L

K

A

E

E

Y

E

E

A

H

K

I

A

V

A

Q

C

T

D

-

G

-

L

-

G

-

P

-

L

-

I

-

I

I

E

D

V

V

P

D

D

E

D

L

Q

M

A

K

S

E

Y

A

P

A

A

E

S

E

G

I

R

E

H

I

P

E

I

A

Y

Y

E

M

D

Q

I

M

L

D

A

A

A

T

A

A

A

T

H

-

D

-

F

-

D

-

W

-

I

-

M

-

P

-

Q

-

D

-

E

-

W

-

E

-

S

-

L

-

A

-

L

L

N

M

Y

Y

T
|
T

S

S

G

G

T

T

G

G

R

K

P

P

K

K

G

G

V

V

V

Q

Y

Q

H

T

H

F

R

G

G

N

A

H

Y

Y

L

F

M
x
S

T

A

M

V

G

S

T

S

V

A

I

L

S
x
N

W

L

R

G

M

I

V

T

M

E

Q

Q

P

D

K

R

Y

W

L

L

A

I

I

A

V
x
L

P
|
P

L

L

F

F

H
|
H

C
x
I

N
x
S

G

G

W

L

N

S

H

A

T
x
L

W

F

M

K

M

S

P

V

V

I

L

Y

G

G

G

M

T

T

L

V

I

V

C
x
L

C

H

R

Q

D

R

I
x
F

T

S

A

V

P

S

A

D

I

V

Y

L

G

H

A

S

I

I

A

N

D

R

E

H

G

E

A

V

T

T

H

M

F

I

G
x
S

G
x
A

A

-

P

-

I

-

V
|
V

L

Q

N

T

M
|
M

L

L

V

A

N

S

A

L

E

E

D

T

R

N

R

R

Q

C

F

P

D

E

H

S

T

I

V

R

E

C

V

I

F
x
L

T

L

A
x
G

G
|
G

A

G

P

P

P

A

A

P

P

L

A

P

T

L

L

L

E

E

K

E

I

C

E

R

A

E

L

K

G

G

F

F

H

P

V

V

T
x
F

Q

Q

V

S

Y

Y

G
|
G

L
x
M

T
|
T

E
|
E

T

T

Y

C

G
x
S

H
x
Q

V

I

T

V

E

-

C
x
T

L
|
L

W
x
S

K

P

G

E

D

F

S

S

W

M

D

E

T

K

L

L

D

G

Q

S

Q

A

G

G

R

K

A

P

A

L

I

F

K

S

A

C

R

-

Q

-

G

-

V

-

A

-

F

-

P

-

M

-

D

-

H

E

I

I

T

K

V

I

M

E

R

R

D

D

D

G

M

Q

Q

-

I

-

A

V

M

C

N

E

G

P

Q

Y

D

E

Q

H

G

G

E

E

I

I

V

M

M

V

R

K

G

G

N

P

S

N

V

V

M

M

K

K

G

S

Y

Y

L

F

K

N

N

R

P

E

D

S

A

A

T

N

A

E

E

A

A

S

F

F

Q

Q

G

N

G

G

Y

W

F

L

H

K

S

T

G

G

D

D

I

L

A

G

V

Y

Q

L

H

D

P

N

D

E

G

G

Y

F

I

L

Q

Y

I

V

A

L

D

D

R

R

A

R

K

S

D

D

I

L

I

I

I
|
I

S
|
S

G
|
G

E
|
E

N
|
N

I

I

S

Y

S

P

V

A

E

E

V

V

E

E

G

S

V

V

L

L

M

L

G

S

H

H

P

P

D

A

V

V

N

A

L

E

A

A

A

G

V

V

V

S

A

G

K

A

P

E

D

D

D

K

K
|
K

W
|
W

G

G

E

K

V

V

P

P

C

H

A

A

F

Y

V

L

E

V

L

L

-

H

K

K

P

P

G

-

A

-

T

-

V

V

D

S

P

A

A

G

D

E

L

L

I

T

R

D

F

Y

A

C

R

K

E

E

T

R

L

L

A

A

G
x
K

F
x
Y

K

K

A

R

P

P

K

K

Q

K

-

F

V

F

V

V

F

L

Q

D

E

R

L

L

P

P

K

R

T

N

S

A

T

S

G

N

K
|
K

I

L

Q

L

K

R

F

N

E

Q

L

L

R

K

Q

D

Q

A

A

R

K

K

active site: T151 (= T192), S171 (≠ M212), H195 (= H236), T288 (= T332), E289 (= E333), I387 (= I445), N392 (= N450), K470 (= K529)
binding magnesium ion: Y23 (= Y41), E24 (≠ G42), H70 (≠ P91), N178 (≠ S219), L202 (≠ T243), L214 (≠ C255), F282 (≠ T326), T296 (≠ C341), L297 (= L342), S298 (≠ W343)
binding o-succinylbenzoyl-N-coenzyme A: K85 (≠ R103), L191 (≠ V232), P192 (= P233), H195 (= H236), I196 (≠ C237), S197 (≠ N238), F218 (≠ I259), S236 (≠ G277), A237 (≠ G278), V238 (= V282), M241 (= M285), L260 (≠ F304), G262 (≠ A306), G263 (= G307), G286 (= G330), M287 (≠ L331), S292 (≠ G336), Q293 (≠ H337), S388 (= S446), G389 (= G447), G390 (= G448), E391 (= E449), K420 (= K478), W421 (= W479), K450 (≠ G510), Y451 (≠ F511)

5gtdA O-succinylbenzoate coa synthetase (mene) from bacillus subtilis in complex with the acyl-adenylate intermediate osb-amp (see paper)
28% identity, 95% coverage: 26:540/542 of query aligns to 8:481/484 of 5gtdA

query
sites
5gtdA

L

L

R

M

R

Q

A

R

A

A

H

Q

V

L

F

T

A

P

D

E

V

R

P

I

A

A

V

L

V

I

Y

Y

G

E

N

D

H

Q

R

T

K

V

T

T

Y

F

A

A

A

E

Y

L

Y

F

D

A

R

A

C

S

T

K

R

R

L

M

A

A

S

E

A

Q

L

L

A

A

G

A

M

H

G

S

V

V

R

R

P

K

G

G

E

D

V

T

V

A

A

A

T

I

L

L

I

L

P

Q

N

N

L

-

P

-

A

-

Q

R

A

A

E

E

A

M

H

V

F

Y

G

A

V

V

P

H

A

A

C

C

-

F

-

L

G

G

A

V

V

K

L

A

N

V

T

L

I

L

N

N

T

T

R

K

L

L

D

S

V

T

S

H

T

E

V

R

A

L

Y

F

I

Q

F

L

D

E

H

D

G

S

E

G

A

S

K

G

V

F

V

L

L

L

V

T

D

D

S

S

Q

S

F

F

L

E

T

K

L

K

A

E

E

Y

E

E

A

H

K

I

A

V

A

Q

C

T

D

-

G

-

L

-

G

-

P

-

L

-

I

-

I

I

E

D

V

V

P

D

D

E

D

L

Q

M

A

K

S

E

Y

A

P

A

A

E

S

E

G

I

R

E

H

I

P

E

I

A

Y

Y

E

M

D

Q

I

M

L

D

A

A

A

T

A

A

A

T

H

-

D

-

F

-

D

-

W

-

I

-

M

-

P

-

Q

-

D

-

E

-

W

-

E

-

S

-

L

-

A

-

L

L

N

M

Y

Y

T
|
T

S

S

G

G

T

T

G

G

R

K

P

P

K

K

G

G

V

V

V

Q

Y

Q

H

T

H

F

R

G

G

N

A

H

Y

Y

L

F

M
x
S

T

A

M

V

G

S

T

S

V

A

I

L

S

N

W

L

R

G

M

I

V

T

M

E

Q

Q

P

D

K

R

Y

W

L

L

A

I

I

A

V
x
L

P

P

L

L

F

F

H
|
H

C
x
I

N
x
S

G

G

W

L

N

S

H

A

T

L

W

F

M

K

M

S

P

V

V

I

L

Y

G

G

G

M

T

T

L

V

I

V

C

L

C

H

R

Q

D

R

I

F

T

S

A

V

P

S

A

D

I

V

Y

L

G

H

A

S

I

I

A

N

D

R

E

H

G

E

A

V

T

T

H

M

F

I

G
x
S

G
x
A

A

-

P

-

I

-

V

V

L

Q

N

T

M

M

L

L

V

A

N

S

A

L

E

E

D

T

R

N

R

R

Q

C

F

P

D

E

H

S

T

I

V

R

E

C

V

I

F
x
L

T

L

A
x
G

G
|
G

A
x
G

P
|
P

P

A

A

P

P

L

A

P

T

L

L

L

E

E

K

E

I

C

E

R

A

E

L

K

G

G

F

F

H

P

V

V

T

F

Q

Q

V
x
S

Y
|
Y

G
|
G

L
x
M

T
|
T

E
|
E

T

T

Y

C

G
x
S

H
x
Q

V

I

T

V

E

-

C

T

L

L

W

S

K

P

G

E

D

F

S

S

W

M

D

E

T

K

L

L

D

G

Q

S

Q

A

G

G

R

K

A

P

A

L

I
x
F

K
x
S

A

C

R

-

Q

-

G

-

V

-

A

-

F

-

P

-

M

-

D

-

H

E

I

I