SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1305 FitnessBrowser__Phaeo:GFF1305 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ryaA Crystal structure of abc transporter solute binding protein avi_3567 from agrobacterium vitis s4, target efi-510645, with bound d-mannitol
71% identity, 95% coverage: 23:433/433 of query aligns to 1:415/417 of 4ryaA

query
sites
4ryaA

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binding d-mannitol: E40 (= E61), R44 (= R65), Y114 (= Y133), E116 (= E135), R163 (= R182), G169 (= G188), F224 (= F243), W279 (= W298), W281 (= W300), Q367 (= Q385)

5iaiA Crystal structure of abc transporter solute binding protein arad_9887 from agrobacterium radiobacter k84, target efi-510945 in complex with ribitol
27% identity, 92% coverage: 25:421/433 of query aligns to 7:392/399 of 5iaiA

query
sites
5iaiA

T

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binding D-ribitol: D15 (≠ N33), D67 (≠ G85), Q176 (≠ A191), W227 (≠ F243), W245 (≠ A261), G283 (≠ A299), W284 (= W300), R357 (≠ D377)

1eu8A Structure of trehalose maltose binding protein from thermococcus litoralis (see paper)
26% identity, 76% coverage: 95:421/433 of query aligns to 79:398/407 of 1eu8A

query
sites
1eu8A

N

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binding alpha-D-glucopyranose: Y120 (= Y133), D122 (≠ E135), G178 (= G188), E238 (≠ N244), W256 (≠ A261), Y258 (≠ V263), G292 (≠ W298), G293 (≠ A299), W294 (= W300), R363 (≠ F386)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 12, 16, 43, 45, 48, 69

Q7LYW7 Trehalose/maltose-binding protein MalE; TMBP from Thermococcus litoralis (strain ATCC 51850 / DSM 5473 / JCM 8560 / NS-C) (see paper)
25% identity, 76% coverage: 95:421/433 of query aligns to 120:439/450 of Q7LYW7

query
sites
Q7LYW7

N

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G

I

F

C

V

L

W

R

Q

G

G

K

K

A

Q

G
x
Y

W

E

G

S

E

L

N

V

A

C

A

D

F

F

I

V

T

E

A

Y

M

V

S

Y

N

S

S

N

F

G

G

G

A

S

-

L

-

G

R

E

W

F

F

K

D

D

E

G

N

K

W

W

A

V

P

P

Q

T

F

L

D

N

S

K

E

P

A

E

W

N

S

V

N

E

T

A

L

L

N

Q

F

F

Y

M

I

V

N

D

L

L

L

I

N

H

D

K

-

Y

-

K

A

I

G

S

P

P

G

N

A

T

S

Y

N

T

N

E

G

M

F

T

N
x
E

E

E

N

P

L

V

S

R

L

L

-

M

F

F

Q

Q

G

G

K

N

C

A

G

A

M

F

W

E

I

R

D

N

A
x
W

T

P

V
x
Y

A

A

A

W

S

G

F

-

V

L

T

H

N

N

P

A

N

D

D

D

S

S

T

P

V

V

A

K

D

G

K

K

V

V

G

G

F

V

A

A

L

P

A

L

P

P

D

H

T

F

G

P

L

G

G

H

K

K

R

S

S

A

N

A

W

T

L

L

W

G

A
x
G

W
|
W

A

H

L

I

A

G

I

I

P

S

A

K

G

Y

T

S

Q

D

K

N

E

K

A

A

A

L

A

A

K

W

E

E

F

F

I

V

Q

K

W

F

A

V

T

E

S

S

K

Y

D

S

Y

V

I

Q

E

K

L

G

V

F

A

A

E

M

N

N

E

L

G

G

W
|
W

A

N

N

-

V

-

P

-

P

P

G

G

A

-

R

R

I

V

S

D

L

V

Y

Y

E

D

N

D

A

P

N

-

Y

-

K

-

D

-

I

-

P

-

F

-

A

-

K

-

M

-

T

-

L

-

E

-

S

A

I

V

L

V

S

S

A

K

D

S

P

P

N

H

N

L

P

K

T

E

V

L

D

R

P

A

V

V

P

F

Y

E

V

N

G

A

I

V

Q

P

F
x
R

A

P

A

I

I

V

P

P

E

Y

F

Y

A

P

G

Q

I

L

A

S

T

E

Q

I

V

I

G

Q

Q

K

E

Y

F

V

S

N

A

S

A

A

L

L

A

A

G

G

Q

K

Q

I

T

S

A

P

E

Q

E

E

A

A

L

L

A

D

K

K

A

A

Q

Q

Y161 (= Y133) binding
D163 (≠ E135) binding
Y217 (≠ G186) binding
E279 (≠ N244) binding
W297 (≠ A261) binding
Y299 (≠ V263) binding
G334 (≠ A299) binding
W335 (= W300) binding
W371 (= W336) binding
R404 (≠ F386) binding

Sites not aligning to the query:

57 binding
84 binding
89 binding
110 binding

7qhvAAA Sulfoquinovosyl binding protein (see paper)
25% identity, 67% coverage: 66:357/433 of query aligns to 47:328/390 of 7qhvAAA

query
sites
7qhvAAA

Q

Q

R

Y

V

L

T

N

T

T

D

V

I

M

S

S

A

A

K

K

G

D

G

S

Q

S

F

L

D

D

I

V

M

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

T

P

P

A

I

Q

W

F

G

A

K

T

N

A

G

G

W

W

L

T

V

S

P

D

L

F

N

S

D

K

L

-

P

-

A

-

D

-

Y

-

D

D

V

L

D

S

D

A

I

Y

L

L

P

P

A

T

M

Y

R

A

G

E

G

A

L

N

S

T

H

V

D

N

G

G

T

K

L

I

F

V

A

A

A

L

P

P

F

A

Y
x
F

G

A

E
x
D

S

S

S

M

M

F

I

L

M

Y

Y

Y

R

R

T

K

D

D

L

L

M

L

E

D

K

K

A

Y

G

G

L

I

E

K

M

P

P

P

A

T

A

-

P

-

T

T

W

W

D

D

F

E

V

L

A

K

D

E

A

A

A

S

R

K

Q

K

M

V

T

M

-

E

-

G

D

E

K

K

D

N

N

P

E

E

T

L

Y

Q

G

G

I

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W
x
E

G
|
G

E

A

N

V

A

C

A

T

F

F

I

L

T

L

A

P

M

Y

S

W

N

S

S

E

F

-

G

-

A

G

R

K

W

S

F

L

D

V

E

E

N

N

W

G

A

K

P

L

Q

N

F

F

D

D

S

N

E

K

A

A

W

A

S

V

N

D

T

S

L

L

N

K

F

L

Y

W

I

K

N

S

L

F

L

V

N

D

D

D

A

G

-

I

G

S

P

K

G

N

A

I

S

S

N

E

N

V

G
x
A

F
x
T

N
x
D

E

D

N

T

L

R

S

K

L

E

F

F

Q

Q

Q

A

G

G

K

K

C

V

G

L

M

F

W

A

I

V

D

N

A
x
W

T

S

V

Y

A

A

A

W

S

T

F

H

V

F

T

Q

N

G

P

-

N

K

D

E

S

S

T

Q

V

V

A

N

D

D

K

K

V

V

G

G

F

V

A

A

L

R

A

L

P

P

D

A

T

V

G

K

L

G

G

G

K

E

R

Q

S

T

N

T

W

C

L

L

W
x
G

A
x
G

W
|
W

A

E

L

F

A

G

I

V

P

S

A

A

G

Y

T

S

Q

K

K

Q

E

Q

A

D

A

E

A

A

K

K

E

K

F

L

I

V

Q

E

W

Y

A

L

T

S

S

S

K

Q

D

D

Y

V

I

S

E

K

L

F

V

M

A

A

E

I

N

N

E

A

G

-

W

-

A

A

N

L

V

L

P

P

P

T

G

Y

A

A

R

-

I

-

S

A

L

L

Y

Y

E

K

N

D

A

A

N

D

Y

V

-

T

K

K

D

T

I

I

P

P

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-[(2~{S})-3-butanoyloxy-2-heptanoyloxy-propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: D66 (≠ G85), F109 (≠ Y133), D111 (≠ E135), E163 (≠ W187), G164 (= G188), A217 (≠ G242), T218 (≠ F243), D219 (≠ N244), W236 (≠ A261), G272 (≠ W298), G273 (≠ A299), W274 (= W300)

Sites not aligning to the query:

binding [(2~{S},3~{S},4~{S},5~{R},6~{S})-6-[(2~{S})-3-butanoyloxy-2-heptanoyloxy-propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 9, 10, 40, 41, 42, 343

A9CEY9 Sulfoquinovosyl glycerol-binding protein SmoF; SQGro-binding protein SmoF; SQ monooxygenase cluster protein F from Agrobacterium fabrum (strain C58 / ATCC 33970) (Agrobacterium tumefaciens (strain C58)) (see 2 papers)
25% identity, 67% coverage: 66:357/433 of query aligns to 76:358/416 of A9CEY9

query
sites
A9CEY9

Q

Q

R

Y

V

L

T

N

T

T

D

V

I

M

S

S

A

A

K

K

G

D

G

S

Q

S

F

L

D

D

I

V

M

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

T

P

P

A

I

Q

W

F

G

A

K

T

N

A

G

G

W

W

L

T

V

-

P

-

L

-

N

S

D

D

L

F

P

S

A

G

D

K

Y

-

D

D

V

L

D

S

D

A

I

Y

L

L

P

P

A

T

M

Y

R

A

G

E

G

A

L

N

S

T

H

V

D

N

G

G

T

K

L

I

F

V

A

A

A

L

P

P

F

A

Y

F

G

A

E
x
D

S

S

S

M

M

F

I

L

M

Y

Y

Y

R

R

T

K

D

D

L

L

M

L

E

D

K

K

A

Y

G

G

L

I

E

K

M

P

P

P

A

T

A

-

P

-

T

T

W

W

D

D

F

E

V

L

A

K

D

E

A

A

A

S

R

K

Q

K

M

V

T

M

-

E

-

G

D

E

K

K

D

N

N

P

E

E

T

L

Y

Q

G

G

I

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W

E

G
|
G

E

A

N

V

A

C

A

T

F

F

I

L

T

L

A

P

M

Y

S

W

N

S

S

E

F

-

G

-

A

G

R

K

W

S

F

L

D

V

E

E

N

N

W

G

A

K

P

L

Q

N

F

F

D

D

S

N

E

K

A

A

W

A

S

V

N

D

T

S

L

L

N

K

F

L

Y

W

I

K

N

S

L

F

L

V

N

D

D

D

A

G

-

I

G

S

P

K

G

N

A

I

S

S

N

E

N

V

G

A

F
x
T

N

D

E

D

N

T

L

R

S

K

L

E

F

F

Q

Q

Q

A

G

G

K

K

C

V

G

L

M

F

W

A

I

V

D

N

A

W

T

S

V

Y

A

A

A

W

S

T

F

H

V

F

T

Q

N

G

P

-

N

K

D

E

S

S

T

Q

V

V

A

N

D

D

K

K

V

V

G

G

F

V

A

A

L

R

A

L

P

P

D

A

T

V

G

K

L

G

G

G

K

E

R

Q

S

T

N

T

W

C

L

L

W

G

A
x
G

W
|
W

A

E

L

F

A

G

I

V

P

S

A

A

G

Y

T

S

Q

K

K

Q

E

Q

A

D

A

E

A

A

K

K

E

K

F

L

I

V

Q

E

W

Y

A

L

T

S

S

S

K

Q

D

D

Y

V

I

S

E

K

L

F

V

M

A

A

E

I

N

N

E

A

G

-

W

-

A

A

N

L

V

L

P

P

P

T

G

Y

A

A

R

-

I

-

S

A

L

L

Y

Y

E

K

N

D

A

A

N

D

Y

V

-

T

K

K

D

T

I

I

P

P

D95 (≠ G85) binding
D141 (≠ E135) binding
G194 (= G188) binding
T248 (≠ F243) binding
G303 (≠ A299) binding ; binding
W304 (= W300) binding ; binding

Sites not aligning to the query:

40 binding
41 binding
71 binding
373 binding ; binding

7yzsAAA Sulfoquinovosyl binding protein (see paper)
25% identity, 67% coverage: 66:357/433 of query aligns to 46:328/384 of 7yzsAAA

query
sites
7yzsAAA

Q

Q

R

Y

V

L

T

N

T

T

D

V

I

M

S

S

A

A

K

K

G

D

G

S

Q

S

F

L

D

D

I

V

M

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

T

P

P

A

I

Q

W

F

G

A

K

T

N

A

G

G

W

W

L

T

V

S

P

D

L

F

N

-

D

-

L

-

P

-

A

S

D

G

Y

K

D

D

V

L

D

S

D

A

I

Y

L

L

P

P

A

T

M

Y

R

A

G

E

G

A

L

N

S

T

H

V

D

N

G

G

T

K

L

I

F

V

A

A

A

L

P

P

F

A

Y

F

G

A

E
x
D

S

S

S

M

M

F

I

L

M

Y

Y

Y

R

R

T

K

D

D

L

L

M

L

E

D

K

K

A

Y

G

G

L

I

E

K

M

P

P

P

A

T

A

-

P

-

T

T

W

W

D

D

F

E

V

L

A

K

D

E

A

A

A

S

R

K

Q

K

M

V

T

M

-

E

-

G

D

E

K

K

D

N

N

P

E

E

T

L

Y

Q

G

G

I

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W
x
E

G
|
G

E

A

N

V

A

C

A

T

F

F

I

L

T

L

A

P

M

Y

S

W

N

S

S

E

F

-

G

-

A

G

R

K

W

S

F

L

D

V

E

E

N

N

W

G

A

K

P

L

Q

N

F

F

D

D

S

N

E

K

A

A

W

A

S

V

N

D

T

S

L

L

N

K

F

L

Y

W

I

K

N

S

L

F

L

V

N

D

D

D

A

G

-

I

G

S

P

K

G

N

A

I

S

S

N

E

N

V

G

A

F
x
T

N

D

E

D

N

T

L

R

S

K

L

E

F

F

Q

Q

Q

A

G

G

K

K

C

V

G

L

M

F

W

A

I

V

D

N

A
x
W

T

S

V

Y

A

A

A

W

S

T

F

H

V

F

T

Q

N

G

P

-

N

K

D

E

S

S

T

Q

V

V

A

N

D

D

K

K

V

V

G

G

F

V

A

A

L

R

A

L

P

P

D

A

T

V

G

K

L

G

G

G

K

E

R

Q

S

T

N

T

W

C

L

L

W
x
G

A
x
G

W
|
W

A

E

L

F

A

G

I

V

P

S

A

A

G

Y

T

S

Q

K

K

Q

E

Q

A

D

A

E

A

A

K

K

E

K

F

L

I

V

Q

E

W

Y

A

L

T

S

S

S

K

Q

D

D

Y

V

I

S

E

K

L

F

V

M

A

A

E

I

N

N

E

A

G

-

W

-

A

A

N

L

V

L

P

P

P

T

G

Y

A

A

R

-

I

-

S

A

L

L

Y

Y

E

K

N

D

A

A

N

D

Y

V

-

T

K

K

D

T

I

I

P

P

binding 6-deoxy-6-sulfo-beta-D-glucopyranose: D65 (≠ G85), D111 (≠ E135), E163 (≠ W187), G164 (= G188), T218 (≠ F243), W236 (≠ A261), G272 (≠ W298), G273 (≠ A299), W274 (= W300)

Sites not aligning to the query:

binding 6-deoxy-6-sulfo-beta-D-glucopyranose: 11, 40, 41, 343

7ofyA Crystal structure of sq binding protein from agrobacterium tumefaciens in complex with sulfoquinovosyl glycerol (sqgro) (see paper)
25% identity, 67% coverage: 66:357/433 of query aligns to 48:330/389 of 7ofyA

query
sites
7ofyA

Q

Q

R

Y

V

L

T

N

T

T

D

V

I

M

S

S

A

A

K

K

G

D

G

S

Q

S

F

L

D

D

I

V

M

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

T

P

P

A

I

Q

W

F

G

A

K

T

N

A

G

G

W

W

L

T

V

S

P

D

L

F

N

-

D

-

L

-

P

-

A

S

D

G

Y

K

D

D

V

L

D

S

D

A

I

Y

L

L

P

P

A

T

M

Y

R

A

G

E

G

A

L

N

S

T

H

V

D

N

G

G

T

K

L

I

F

V

A

A

A

L

P

P

F

A

Y

F

G

A

E
x
D

S

S

S

M

M

F

I

L

M

Y

Y

Y

R

R

T

K

D

D

L

L

M

L

E

D

K

K

A

Y

G

G

L

I

E

K

M

P

P

P

A

T

A

-

P

-

T

T

W

W

D

D

F

E

V

L

A

K

D

E

A

A

A

S

R

K

Q

K

M

V

T

M

-

E

-

G

D

E

K

K

D

N

N

P

E

E

T

L

Y

Q

G

G

I

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W

E

G
|
G

E

A

N

V

A

C

A

T

F

F

I

L

T

L

A

P

M

Y

S

W

N

S

S

E

F

-

G

-

A

G

R

K

W

S

F

L

D

V

E

E

N

N

W

G

A

K

P

L

Q

N

F

F

D

D

S

N

E

K

A

A

W

A

S

V

N

D

T

S

L

L

N

K

F

L

Y

W

I

K

N

S

L

F

L

V

N

D

D

D

A

G

-

I

G

S

P

K

G

N

A

I

S

S

N

E

N

V

G

A

F
x
T

N

D

E

D

N

T

L

R

S

K

L

E

F

F

Q

Q

Q

A

G

G

K

K

C

V

G

L

M

F

W

A

I

V

D

N

A
x
W

T

S

V
x
Y

A

A

A

W

S

T

F

H

V

F

T

Q

N

G

P

-

N

K

D

E

S

S

T

Q

V

V

A

N

D

D

K

K

V

V

G

G

F

V

A

A

L

R

A

L

P

P

D

A

T

V

G

K

L

G

G

G

K

E

R

Q

S

T

N

T

W

C

L

L

W
x
G

A
x
G

W
|
W

A

E

L

F

A

G

I

V

P

S

A

A

G

Y

T

S

Q

K

K

Q

E

Q

A

D

A

E

A

A

K

K

E

K

F

L

I

V

Q

E

W

Y

A

L

T

S

S

S

K

Q

D

D

Y

V

I

S

E

K

L

F

V

M

A

A

E

I

N

N

E

A

G

-

W

-

A

A

N

L

V

L

P

P

P

T

G

Y

A

A

R

-

I

-

S

A

L

L

Y

Y

E

K

N

D

A

A

N

D

Y

V

-

T

K

K

D

T

I

I

P

P

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: D67 (≠ G85), D113 (≠ E135), G166 (= G188), T220 (≠ F243), W238 (≠ A261), Y240 (≠ V263), G274 (≠ W298), G275 (≠ A299), W276 (= W300)

Sites not aligning to the query:

binding [(2S,3S,4S,5R,6S)-6-[(2R)-2,3-bis(oxidanyl)propoxy]-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 12, 42, 43, 345

6dtqA Maltose bound t. Maritima male3 (see paper)
25% identity, 73% coverage: 101:417/433 of query aligns to 78:380/391 of 6dtqA

query
sites
6dtqA

L

L

N

E

D

D

L

L

P

T

A

A

D

D

-

K

-

D

-

Y

Y

F

D

E

V

L

D

G

D

E

I

F

L

L

P

P

A

G

M

T

R

V

G

M

G

S

L

V

S

T

H

V

D

N

G

G

T

R

L

I

F

V

A

A

A

V

P

P

F

W

Y

F

G

T

E
x
D

S

A

S

G

M

L

I

L

M

Y

Y

Y

R

R

T

K

D

D

L

L

M

L

E

E

K

K

A

Y

G

G

L

Y

E

D

M

H

P

-

A

A

A

P

P

R

T

T

W

W

D

D

F

E

V

L

A

V

D

E

A

M

A

A

R

K

Q

K

M

I

T

S

D

Q

K

A

D

E

N

G

E

-

T

I

Y

H

G

G

I

F

C

V

L

W

R

Q

G

G

K

A

A

R

G

Y

W

E

G
|
G

E

L

N

V

A

C

A

D

F

F

I

L

T

E

A

Y

M

L

S

W

N

-

S

S

F

F

G

G

A

G

R

D

W

V

F

L

D

D

E

E

N

S

W

G

A

K

P

V

Q

V

F

I

D

D

S

S

E

P

A

E

W

A

S

V

N

A

T

A

L

L

N

Q

F

F

Y

M

I

V

N

D

L

L

L

I

-

Y

-

K

N

H

D

K

A

V

G

T

P

P

P

E

G

G

A

V

S

T

N

T

N

Y

G

M

F
x
E

N

E

E

D

N

A

L

R

S

R

L

I

F

F

Q

Q

Q

N

G

G

K

E

C

A

G

V

M

F

W

M

I

R

D

N

A
x
W

T

P

V

Y

A

A

A

W

S

S

F

L

V

V

T

-

N

N

P

S

N

D

D

E

S

S

T

P

V

I

A

K

D

G

K

K

V

V

G

G

F

V

A

A

L

P

A

L

P

P

D

M

T

G

G

P

L

G

G

G

K

R

R

R

S

A

N

A

W

T

L

L

W

G

A
x
G

W
|
W

A

V

L

L

A

G

I

I

P

N

-

K

-

F

A

S

G

S

T

P

Q

E

K
x
E

E

K

A

E

A

A

K

K

E

K

F

L

I

I

Q

K

W

F

A

L

T

T

S

S

K

Y

D

D

Y

Q

I

Q

E

L

L

Y

V

K

A

A

E

I

N

N

E

A

G

G

W
x
Q

A

N

N

P

V

-

P

-

G

-

A

T

R

R

I

K

S

A

L

V

Y

Y

E

K

N

D

A

P

N

K

Y

L

K

K

D

E

-

A

I

A

P

P

F

F

A

M

K

V

M

E

T

L

L

L

E

G

S

V

I

F

L

I

S

N

A

A

D

L

P

P

N
x
R

N

-

P

-

T

-

V

-

D

-

P

-

V

-

P

-

Y

-

V

-

G

-

I

-

Q

-

F

-

A

P

A

R

I

V

P

A

E

N

F

Y

A

T

G

E

I

V

A

S

T

D

Q

V

V

I

G

Q

Q

R

E

Y

F

V

S

H

A

A

L

L

A

T

G

R

Q

Q

Q

T

T

T

A

S

E

E

E

D

A

A

L

I

binding alpha-D-glucopyranose: D115 (≠ E135), D115 (≠ E135), G166 (= G188), E222 (≠ F243), W240 (≠ A261), W240 (≠ A261), G277 (≠ A299), W278 (= W300), W278 (= W300), Q316 (≠ W336), R349 (≠ N372)
binding magnesium ion: E290 (≠ K310)

Sites not aligning to the query:

binding alpha-D-glucopyranose: 11, 14, 46, 67
binding magnesium ion: 59, 60, 62

5ci5A Crystal structure of an abc transporter solute binding protein from thermotoga lettingae tmo (tlet_1705, target efi-510544) bound with alpha-d-tagatose
23% identity, 88% coverage: 42:420/433 of query aligns to 22:383/393 of 5ci5A

query
sites
5ci5A

L

V

T

I

E

K

D

T

F

F

T

E

A

E

K

K

T

Y

G

G

H

I

E

S

V

V

E

E

W

V

V

Q

T

L

L

F

E

G

E
x
F

N

D

V

T

L

Y

R

Y

Q

D

R

K

V

L

T

V

T

T

D

A

I

L

S

Q

A

A

-

G

K

K

G

G

G

P

Q

D

F

L

D

A

I

F

M

A

T
x
D

I

L

G

G

M

G

Y

W

E

-

T

V

P

P

I

T

W

F

G

A

K

E

N

K

G

G

W

W

L

L

V

E

P

P

L

M

N

E

D

E

L

H

P

L

A

K

D

N

Y

W

D

E

-

G

V

T

D

A

D

Q

I

I

L

W

P

P

A

N

M

L

R

W

G

P

G

T

L

V

S

T

H

Y

D

K

G

K

T

I

L

R

F

Y

A

G

A

L

P

P

F

W

Y
|
Y

G

T

E
x
D

S

C

S
x
R

M

L

I

L

M

L

Y

Y

R

N

T

K

D

A

L

M

M

F

E

E

K

K

A

A

G

G

L

L

E

N

M

P

P

D

A

N

A

P

P

P

-

K

T

T

W

W

D

D

F

E

V

L

A

L

D

D

A

A

R

L

Q

K

M

I

T

T

D

D

K

T

D

K

N

N

E

R

T

I

Y

Y

G

G

I

Y

C

G

L

V

R

S

G

G

K

T

A

K

G

T

W

E

G

H

E

T

N

T

A

L

A

G

F

Y

I

M

T

M

A

F

M

L

S

Y

N

A

S

A

F

G

G

G

A

K

R

L

W

L

F

T

D

D

E

D

N

Y

W

S

A

K

P

A

Q

A

F

F

D

D

S

S

E

P

A

E

W

G

S

L

N

K

T

A

L

L

N

K

F

F

Y

Y

I

T

N

D

L

L

L

A

N

K

D

K

A

Y

G

N

-

V

P

S

P

P

G

N

A

A

S

I

N

Q

N

Y

G

H

F

E

N

D

E

D

N

Y

L

R

S

N

L

M

F

M

Q

A

Q

Q

G

N

K

R

C

V

G

A

M

M

W

A

I

I

D

G

A

G

T

P

V
x
W

A

S

A

F

S

P

F

L

V

I

T

E

N

A

P

A

N

N

D

P

S

D

T

-

V

I

A

A

D

G

K

K

V

Y

G

S

F

V

A

A

L

L

A

H

P

P

D

Y

T

D

G

-

L

-

G

A

K

K

R

P

S

A

N

S

W

V

L

L

W
x
G

A
x
G

W
|
W

A

A

L

L

A

V

I

I

P

P

A

S

G

S

T

S

Q

P

K

N

E

K

A

E

A

D

A

A

K

W

E

K

F

L

I

A

Q

E

W

Y

A

L

T

T

S

S

K

F

D

D

-

V

Y

W

I

M

E

K

L

W

V

V

A

E

E

E

N

K

E

G

G

G

W

-

A

-

N

-

V

-

P

P

P

M

G

P

A

T

R

R

I

M

S

D

L

V

Y

C

E

K

N

K

A

S

N

K

Y

L

K

A

D

N

I

D

P

V

F

K

A

W

K

Q

M

I

T

I

L

F

E

E

S

T

I

F

L

P

S

H

A

A

D

V

P

A

N
x
R

N

P

P

P

T

-

V

-

D

-

P

-

V

-

P

-

Y

-

V

-

G

-

I

-

Q

-

F

-

A

-

I

I

P

P

E

Q

F

Y

A

P

G

Q

I

I

A

S

T

E

Q

Q

V

I

G

Q

Q

T

E

M

F

V

S

Q

A

R

A

V

L

L

A

L

G

G

Q

E

Q

L

T

T

A

P

E

E

A

A

L

I

A

K

K

I

A

A

binding alpha-D-tagatopyranose: F41 (≠ E61), D64 (≠ T83), Y114 (= Y133), D116 (≠ E135), R118 (≠ S137), W246 (≠ V263), G278 (≠ W298), G279 (≠ A299), W280 (= W300), R349 (≠ N372)

Sites not aligning to the query:

binding alpha-D-tagatopyranose: 11

7yzuA Crystal structure of the sulfoquinovosyl binding protein smof complexed with sqme (see paper)
25% identity, 67% coverage: 66:357/433 of query aligns to 45:325/382 of 7yzuA

query
sites
7yzuA

Q

Q

R

Y

V

L

T

N

T

T

D

V

I

M

S

S

A

A

K

K

G

D

G

S

Q

S

F

L

D

D

I

V

M

L

T

M

I

L

G
x
D

M

V

Y

I

E

R

T

P

P

A

I

Q

W

F

G

A

K

T

N

A

G

G

W

W

L

T

V

-

P

-

L

-

N

S

D

D

L

F

P

S

A

G

D

K

Y

-

D

D

V

L

D

S

D

A

I

Y

L

L

P

P

A

T

M

Y

R

A

G

E

G

A

L

N

S

T

H

V

D

N

G

G

T

K

L

I

F

V

A

A

A

L

P

P

F

A

Y

F

G

A

E
x
D

S

S

S

M

M

F

I

L

M

Y

Y

Y

R

R

T

K

D

D

L

L

M

L

E

D

K

K

A

Y

G

G

L

I

E

K

M

P

P

P

A

T

A

-

P

-

T

T

W

W

D

D

F

E

V

L

A

K

D

E

A

A

A

S

R

K

Q

K

M

V

T

M

-

E

-

G

D

E

K

K

D

N

N

P

E

E

T

L

Y

Q

G

G

I

L

C

S

L

F

R

Q

G

G

K

K

A

A

G

I

W
x
E

G
|
G

E

A

N

V

A

C

A

T

F

F

I

L

T

L

A

P

M

Y

S

W

N

S

S

E

F

G

G

K

A

S

R

L

W

V

F

G

D

K

E

L

N

N

W

-

A

-

P

-

Q

-

F

F

D

D

S

N

E

K

A

A

W

A

S

V

N

D

T

S

L

L

N

K

F

L

Y

W

I

K

N

S

L

F

L

V

N

D

D

D

A

G

-

I

G

S

P

K

G

N

A

I

S

S

N

E

N

V

G

A

F
x
T

N

D

E

D

N

T

L

R

S

K

L

E

F

F

Q

Q

Q

A

G

G

K

K

C

V

G

L

M

F

W

A

I

V

D

N

A
x
W

T

S

V

Y

A

A

A

W

S

T

F

H

V

F

T

Q

N

G

P

-

N

K

D

E

S

S

T

Q

V

V

A

N

D

D

K

K

V

V

G

G

F

V

A

A

L

R

A

L

P

P

D

A

T

V

G

K

L

G

G

G

K

E

R

Q

S

T

N

T

W

C

L

L

W
x
G

A
x
G

W
|
W

A

E

L

F

A

G

I

V

P

S

A

A

G

Y

T

S

Q

K

K

Q

E

Q

A

D

A

E

A

A

K

K

E

K

F

L

I

V

Q

E

W

Y

A

L

T

S

S

S

K

Q

D

D

Y

V

I

S

E

K

L

F

V

M

A

A

E

I

N

N

E

A

G

-

W

-

A

A

N

L

V

L

P

P

P

T

G

Y

A

A

R

-

I

-

S

A

L

L

Y

Y

E

K

N

D

A

A

N

D

Y

V

-

T

K

K

D

T

I

I

P

P

binding [(2S,3S,4S,5R,6S)-6-methoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: D64 (≠ G85), D110 (≠ E135), E162 (≠ W187), G163 (= G188), T215 (≠ F243), W233 (≠ A261), G269 (≠ W298), G270 (≠ A299), W271 (= W300)

Sites not aligning to the query:

binding [(2S,3S,4S,5R,6S)-6-methoxy-3,4,5-tris(oxidanyl)oxan-2-yl]methanesulfonic acid: 9, 39, 40, 340

5ysbA Crystal structure of beta-1,2-glucooligosaccharide binding protein in ligand-free form (see paper)
23% identity, 86% coverage: 39:409/433 of query aligns to 15:359/386 of 5ysbA

query
sites
5ysbA

M

L

Q

K

G
x
E

L

L

T

A

E

Q

D

K

F

F

T

T

A

K

K

D

T

T

G

G

H

I

E

E

V

V

E

K

W

V

V

Q

T

V

L

I

E

P

E

W

N

A

V

N

L

A

R
x
H

Q

D

R

K

V

L

T

L

T

T

D

A

I

V

S

A

A

S

K

K

G

S

G

G

Q

P

F

-

D

D

I

V

M

V

T

Q

I

M

G

G

M

T

Y

T

E

W

T

M

P

P

I
x
E

W

F

G

V

K
x
E

N

A

G

G

W

A

L

L

V

L

P

D

L

I

N

T

-

K

-

D

-

V

D

E

L

K

P

S

A

K

D

N

Y

M

D

N

V

S

D

D

D

L

I

F

L

F

P

P

A

G

M

S

R

V

G

K

G

T

L

T

S

Q

H

F

D

D

G

G

T

K

L

T

F

Y

A

G

A

V

P

P

F

W

Y

Y

G

A

E

E

S

T

S

R

M

V

I

L

M

F

Y

Y

R

R

T

T

D

D

L

L

M

L

E

K

K

K

A

V

G

G

L

Y

E

N

M

-

P

E

A

A

A

P

P

K

T

T

W

W

D

D

F

E

V

L

A

S

D

D

A

A

R

L

Q

K

M

L

T

S

D

K

K

R

D

G

N

K

E

D

T

M

Y

Y

G

G

I

F

C

A

L

I

R

D

G

P

K

N

A

E

G

-

W

-

G

-

E

Q

N

T

A

T

A

G

F

F

I

I

T

F

A

G

M

R

S

Q

N

N

S

-

F

-

G

G

A

S

R

P

W

L

F

F

D

D

E

K

N

D

W

G

A

T

P

P

Q

V

F

F

D

N

S

K

E

K

A

P

W

F

S

V

N

D

T

T

L

V

N

T

F

Y

Y

L

I

D

N

S

L

F

L

I

N

K

D

N

A

G

G

S

P

A

P

P

G

D

A

-

S

T

N

D

N

L

G

G

F

L

N

D

E

A

N

S

L

Q

S

S

L

F

F

G

Q

G

Q

D

G

G

K

I

C

V

G

P

M

M

W

F

I

M

D

-

A

-

T

-

V

-

A

S

A

G

S

P

F

W

V

M

T

V

N
|
N

P

T

N

L

D

K

S
x
D

T

T

V

A

A

P

D

D

-

I

-

D

-

G

K

K

V

W

G

A

F

T

A

A

L

V

A

L

P

P

D

K

T

K

G

E

L

N

G

N

K

E

R

S

S

S

N

-

W

-

L

L

W

G

A

G

W

A

A

N

L

L

A

S

I

I

P

F

A

K

G

Y

T

S

Q

N

K

K

E

K

A

D

A

A

K

L

E

K

F

F

I

M

Q

D

W

Y

A

M

T

S

S

Q

K

P

D

D

Y

-

I

V

E

Q

L

L

V

S

A

W

E

L

N

K

E
x
D

G

-

W

-

A

T

N

N

V

S

P

M

P

P

G

-

A

A

R

R

I

M

S

D

L

A

Y

W

E

E

N

D

A

D

N

M

Y

L

K

K

D

N

I

D

P

P

F

Y

A

Y

K

K

M

V

T

F

L

G

E

E

S

Q

I

M

L

K

S

T

A

A

D

E

P

P

N

-

P

-

T

-

V

-

D

-

P

-

V

-

P

-

Y

-

V

-

G

-

I

-

Q

-

F

M

A

P

A

L

I

I

P

P

E

Q

F

F

A

E

G

E

I

I

A

A

T

Q

Q

L

V

Y

G

G

Q

K

E

S

F

W

S

E

A

Q

A

I

L

Y

A

R

G

G

binding zinc ion: E17 (≠ G41), H41 (≠ R65), E66 (≠ I91), E69 (≠ K94), N237 (= N270), D241 (≠ S274), D301 (≠ E334)

Sites not aligning to the query:

binding zinc ion: 384, 385

5ysdB Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotriose (see paper)
23% identity, 86% coverage: 39:409/433 of query aligns to 16:360/389 of 5ysdB

query
sites
5ysdB

M

L

Q

K

G

E

L

L

T

A

E

Q

D

K

F

F

T

T

A

K

K

D

T

T

G

G

H

I

E

E

V

V

E

K

W

V

V

Q

T

V

L

I

E

P

E
x
W

N

A

V

N

L

A

R

H

Q

D

R

K

V

L

T

L

T

T

D

A

I

V

S

A

A

S

K

K

G

S

G

G

Q

P

F

-

D

D

I

V

M

V

T

Q

I

M

G
|
G

M
x
T

Y
x
T

E
x
W

T

M

P

P

I

E

W

F

G

V

K

E

N

A

G

G

W

A

L

L

V

L

P

D

L

I

N

T

-

K

-

D

-

V

D

E

L

K

P

S

A

K

D

N

Y

M

D

N

V

S

D

D

D

L

I

F

L

F

P

P

A

G

M

S

R

V

G

K

G

T

L

T

S

Q

H

F

D

D

G

G

T

K

L

T

F

Y

A

G

A

V

P

P

F

W

Y
|
Y

G

A

E
|
E

S

T

S

R

M

V

I

L

M

F

Y

Y

R

R

T

T

D

D

L

L

M

L

E

K

K

K

A

V

G

G

L

Y

E

N

M

-

P

E

A

A

A

P

P

K

T

T

W

W

D

D

F

E

V

L

A

S

D

D

A

A

R

L

Q

K

M

L

T

S

D

K

K

R

D

G

N

K

E

D

T

M

Y

Y

G

G

I

F

C

A

L

I

R
x
D

G

P

K

N

A

E

G

-

W

-

G

-

E
x
Q

N

T

A

T

A

G

F

F

I

I

T

F

A

G

M

R

S

Q

N

N

S

-

F

-

G

G

A

S

R

P

W

L

F

F

D

D

E

K

N

D

W

G

A

T

P

P

Q

V

F

F

D

N

S

K

E

K

A

P

W

F

S

V

N

D

T

T

L

V

N

T

F

Y

Y

L

I

D

N

S

L

F

L

I

N

K

D

N

A

G

G

S

P

A

P

P

G

D

A

-

S

T

N

D

N

L

G

G

F

L

N

D

E

A

N

S

L

Q

S

S

L

F

F

G

Q

G

Q

D

G

G

K

I

C

V

G

P

M

M

W

F

I

M

D

-

A

-

T

-

V

-

A

S

A

G

S

P

F
x
W

V

M

T

V

N

N

P

T

N

L

D

K

S

D

T

T

V

A

A

P

D

D

-

I

-

D

-

G

K

K

V

W

G

A

F

T

A

A

L

V

A

L

P

P

D

K

T

K

G

E

L

N

G

N

K

E

R

S

S

S

N

-

W

-

L

L

W

G

A
x
G

W

A

A

N

L

L

A

S

I

I

P

F

A

K

G

Y

T

S

Q

N

K

K

E

K

A

D

A

A

K

L

E

K

F

F

I

M

Q

D

W

Y

A

M

T

S

S

Q

K

P

D

D

Y

-

I

V

E

Q

L

L

V

S

A

W

E

L

N

K

E

D

G

-

W

-

A

T

N

N

V

S

P

M

P

P

G

-

A

A

R

R

I

M

S

D

L

A

Y

W

E

E

N

D

A

D

N

M

Y

L

K

K

D

N

I

D

P

P

F

Y

A

Y

K

K

M

V

T

F

L

G

E

E

S

Q

I

M

L

K

S

T

A

A

D

E

P

P

N

-

P

-

T

-

V

-

D

-

P

-

V

-

P

-

Y

-

V

-

G

-

I

-

Q

-

F

M

A

P

A

L

I

I

P

P

E

Q

F

F

A

E

G

E

I

I

A

A

T

Q

Q

L

V

Y

G

G

Q

K

E

S

F

W

S

E

A

Q

A

I

L

Y

A

R

G

G

binding beta-D-glucopyranose: W38 (≠ E61), W38 (≠ E61), G61 (= G85), T62 (≠ M86), T63 (≠ Y87), W64 (≠ E88), Y112 (= Y133), E114 (= E135), W235 (≠ F267), G268 (≠ A299)
binding alpha-D-glucopyranose: W38 (≠ E61), D160 (≠ R182), Q164 (≠ E189)

Sites not aligning to the query:

binding beta-D-glucopyranose: 12

5ysdA Crystal structure of beta-1,2-glucooligosaccharide binding protein in complex with sophorotriose (see paper)
23% identity, 86% coverage: 39:409/433 of query aligns to 16:360/387 of 5ysdA

query
sites
5ysdA

M

L

Q

K

G

E

L

L

T

A

E

Q

D

K

F

F

T

T

A

K

K

D

T

T

G

G

H

I

E

E

V

V

E

K

W

V

V

Q

T

V

L

I

E

P

E
x
W

N

A

V

N

L

A

R

H

Q

D

R

K

V

L

T

L

T

T

D

A

I

V

S

A

A

S

K

K

G

S

G

G

Q

P

F

-

D

D

I

V

M

V

T

Q

I

M

G
|
G

M
x
T

Y
x
T

E
x
W

T

M

P

P

I

E

W

F

G

V

K

E

N

A

G

G

W

A

L

L

V

L

P

D

L

I

N

T

-

K

-

D

-

V

D

E

L

K

P

S

A

K

D

N

Y

M

D

N

V

S

D

D

D

L

I

F

L

F

P

P

A

G

M

S

R

V

G

K

G

T

L

T

S

Q

H

F

D

D

G

G

T

K

L

T

F

Y