SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1369 FitnessBrowser__Marino:GFF1369 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 6 hits to proteins with known functional sites (download)

Q02I31 Putative pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
53% identity, 91% coverage: 5:112/119 of query aligns to 1:108/118 of Q02I31

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Q02I31

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M1 (= M5) modified: Initiator methionine, Removed
T2 (≠ S6) modified: Phosphothreonine

2v6tB Crystal structure of a complex of pterin-4a-carbinolamine dehydratase from toxoplasma gondii with 7,8-dihydrobiopterin (see paper)
33% identity, 71% coverage: 30:114/119 of query aligns to 13:95/100 of 2v6tB

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2v6tB

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active site: D54 (≠ E73), H58 (= H77), H59 (= H78), H76 (= H95), D77 (≠ E96), D85 (= D104)
binding 2-amino-6-(1,2-dihydroxy-propyl)-7,8-dihydro-6h-pteridin-4-one: D57 (= D76), H58 (= H77), H59 (= H78), D77 (≠ E96)

1f93A Crystal structure of a complex between the dimerization domain of hnf-1 alpha and the coactivator dcoh (see paper)
30% identity, 76% coverage: 20:109/119 of query aligns to 4:93/103 of 1f93A

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1f93A

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active site: E57 (= E73), H61 (= H77), H62 (= H78), H79 (= H95), E80 (= E96), D88 (= D104)
binding : N43 (≠ V59), R44 (≠ K60), G47 (≠ E63), R51 (≠ K67), L54 (≠ E70)

P61459 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Rattus norvegicus (Rat) (see 2 papers)
30% identity, 76% coverage: 20:109/119 of query aligns to 5:94/104 of P61459

query
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C82 (≠ I97) mutation to R: Reduced dehydratase activity. No impact on hydroxytetrahydrobiopterin-binding.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine

P61457 Pterin-4-alpha-carbinolamine dehydratase; PHS; 4-alpha-hydroxy-tetrahydropterin dehydratase; Dimerization cofactor of hepatocyte nuclear factor 1-alpha; DCoH; Dimerization cofactor of HNF1; Phenylalanine hydroxylase-stimulating protein; Pterin carbinolamine dehydratase; PCD; EC 4.2.1.96 from Homo sapiens (Human) (see 6 papers)
30% identity, 76% coverage: 20:109/119 of query aligns to 5:94/104 of P61457

query
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T79 (≠ S94) to I: in HPABH4D; increased proteolytic degradation; reduced interaction with HNF1B; loss of HNF1B-coactivator activity; dbSNP:rs121913014
C82 (≠ I97) to R: in HPABH4D; increased proteolytic degradation; reduced dehydratase activity; no impact on hydroxytetrahydrobiopterin-binding; reduced interaction with HNF1B; partial impact on HNF1B-coactivator activity; dbSNP:rs104894177
R88 (≠ N103) to Q: in HPABH4D; when associated in cis with 27-E--T-104 del; uncertain significance; no impact on HNF1B interaction; no impact on HNF1B-coactivator activity; dbSNP:rs115117837

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylalanine
27:104 natural variant: Missing (in HPABH4D; when associated in cis with Q-88; reduced protein levels; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs727505360)
87:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs104894172)
97 E → K: in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs397518416
98:104 natural variant: Missing (in HPABH4D; increased proteolytic degradation; loss of HNF1B interaction; loss of HNF1B-coactivator activity; dbSNP:rs121913015)

1dcpA Dcoh, a bifunctional protein-binding transcriptional coactivator, complexed with biopterin (see paper)
30% identity, 74% coverage: 22:109/119 of query aligns to 2:89/99 of 1dcpA

query
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1dcpA

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active site: E53 (= E73), H57 (= H77), H58 (= H78), H75 (= H95), E76 (= E96), D84 (= D104)
binding 7,8-dihydrobiopterin: D56 (= D76), H57 (= H77), H58 (= H78), T74 (≠ S94), H75 (= H95)

Query Sequence

>GFF1369 FitnessBrowser__Marino:GFF1369
MEVEMSDLKAHSCEACSPNASKATEEEKQQLHKEVPDWEILEIDGEEQLHKVYKLKNFVK
ALELTNKIGELAEEEDHHPVVVLEYGKVAVSWWSHEIGGLHKNDFILAARTDAAFNDMQ

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory