SitesBLAST

Comparing GFF1376 HP15_1343 2-hydroxy-3-oxopropionate reductase to proteins with known functional sites using BLASTp with E ≤ 0.001.

Found 20 (the maximum) hits to proteins with known functional sites

3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
37% identity, 99% coverage: 2:289/290 of query aligns to 1:287/287 of 3pefA

• binding glycerol: D67 (≠ S69), G123 (= G125), S124 (≠ T126), K171 (= K173), N175 (= N177), M178 (≠ L180), L203 (≠ M205), G207 (≠ P209), A212 (≠ T214), N213 (≠ P215), F216 (≠ I218), A217 (≠ G219), F232 (= F234), K235 (≠ E237), H236 (≠ L238), K239 (= K241), R242 (≠ H244), R269 (≠ S271)
• binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), L9 (= L10), G10 (= G11), I11 (= I12), M12 (= M13), N31 (= N33), R32 (= R34), S33 (= S35), K36 (≠ A38), M64 (= M66), L65 (= L67), A66 (= A68), A70 (≠ V72), E73 (≠ D75), V74 (≠ M76), S95 (= S97), T96 (= T98), V121 (= V123), G123 (= G125), S124 (≠ T126), K171 (= K173), A231 (≠ Q233), F232 (= F234), P233 (= P235), H236 (≠ L238), K239 (= K241)

3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
35% identity, 98% coverage: 4:287/290 of query aligns to 3:285/287 of 3pduA

• binding glycerol: R242 (≠ H244), E246 (≠ Q248), E246 (≠ Q248), D249 (≠ Y251), R250 (≠ E252)
• binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G9), L9 (= L10), G10 (= G11), I11 (= I12), M12 (= M13), N31 (= N33), R32 (= R34), N33 (≠ S35), M64 (= M66), L65 (= L67), A66 (= A68), A70 (≠ V72), E73 (≠ D75), V74 (≠ M76), S95 (= S97), T96 (= T98), V121 (= V123), G123 (= G125), T124 (= T126), K171 (= K173), S231 (≠ Q233), F232 (= F234), P233 (= P235), H236 (≠ L238), K239 (= K241), D240 (= D242)

2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
34% identity, 99% coverage: 5:290/290 of query aligns to 3:294/296 of 2i9pB

• binding nicotinamide-adenine-dinucleotide: I6 (= I8), G7 (= G9), L8 (= L10), G9 (= G11), N10 (≠ I12), M11 (= M13), Y29 (≠ F32), D30 (≠ N33), V31 (≠ R34), F32 (≠ S35), M63 (= M66), L64 (= L67), P65 (≠ A68), N69 (≠ V72), A73 (≠ M76), S94 (= S97), T95 (= T98), V120 (= V123), G122 (= G125), G123 (≠ T126), K170 (= K173), F238 (= F234), L242 (= L238), K245 (= K241), D246 (= D242)

P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
34% identity, 99% coverage: 5:290/290 of query aligns to 42:333/336 of P31937

• LP 103:104 (≠ LA 67:68) binding
• N108 (≠ V72) binding
• T134 (= T98) binding
• K284 (= K241) binding

Sites not aligning to the query:

• 1:36 modified: transit peptide, Mitochondrion
• 40:68 binding

P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
33% identity, 98% coverage: 5:287/290 of query aligns to 41:329/335 of P29266

• D68 (≠ N33) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
• K208 (= K173) mutation K->A,H,N,R: Complete loss of activity.
• N212 (= N177) mutation to Q: Decrease in activity.

P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
33% identity, 97% coverage: 5:285/290 of query aligns to 4:284/298 of P0A9V8

• QM 11:12 (≠ IM 12:13) binding
• D31 (≠ N33) binding
• L65 (= L67) binding
• T96 (= T98) binding
• G122 (≠ A124) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
• R123 (≠ G125) binding ; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
• T124 (= T126) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
• NNYMS 174:178 (≠ VNAML 176:180) binding
• K240 (= K241) binding

6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
33% identity, 97% coverage: 5:285/290 of query aligns to 3:283/294 of 6smyA

• binding (2~{S})-2,3-bis(oxidanyl)propane-1-sulfonic acid: T208 (≠ L211), F232 (= F234), W278 (≠ F280)

6smzC Crystal structure of sla reductase yihu from e. Coli in complex with nadh
33% identity, 97% coverage: 5:285/290 of query aligns to 3:283/295 of 6smzC

• binding nicotinamide-adenine-dinucleotide: G7 (= G9), L8 (= L10), G9 (= G11), Q10 (≠ I12), M11 (= M13), F29 (= F32), D30 (≠ N33), V31 (≠ R34), N32 (≠ S35), M63 (= M66), L64 (= L67), P65 (≠ A68), L69 (≠ V72), V73 (≠ M76), S94 (= S97), T95 (= T98), V120 (= V123), R122 (≠ G125)

Q8T079 Cytokine-like nuclear factor N-PAC; NPAC; Glyoxylate reductase 1 homolog; Nuclear protein NP60 homolog; Nucleosome-destabilizing factor; Putative oxidoreductase GLYR1 homolog from Drosophila melanogaster (Fruit fly) (see paper)
28% identity, 97% coverage: 3:284/290 of query aligns to 316:597/602 of Q8T079

Sites not aligning to the query:

• 8 modified: Phosphoserine
• 10 modified: Phosphoserine
• 224 modified: Phosphoserine
• 228 modified: Phosphoserine
• 243 modified: Phosphoserine

3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
34% identity, 94% coverage: 5:276/290 of query aligns to 16:283/293 of 3ws7A

• binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G9), L21 (= L10), G22 (= G11), I23 (= I12), M24 (= M13), N43 (= N33), R44 (= R34), T45 (≠ S35), K48 (≠ A38), M76 (= M66), V77 (≠ L67), S78 (≠ A68), D82 (≠ V72), Q85 (≠ D75), V86 (≠ M76), S107 (= S97), T108 (= T98), V133 (= V123), G136 (≠ T126), K183 (= K173), G240 (≠ Q233), F241 (= F234), K242 (≠ P235), H245 (≠ L238), K248 (= K241), D249 (= D242)
• binding sulfate ion: T134 (≠ A124), G135 (= G125), G136 (≠ T126), K183 (= K173), N186 (≠ V176), V190 (≠ L180)

2uyyA Structure of the cytokine-like nuclear factor n-pac
29% identity, 98% coverage: 3:285/290 of query aligns to 7:288/292 of 2uyyA

• binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: L14 (= L10), G15 (= G11), L16 (≠ I12), M17 (= M13), W35 (≠ F32), N36 (= N33), R37 (= R34), T38 (≠ S35), C69 (≠ M66), V70 (≠ L67), S71 (≠ A68), A75 (≠ V72), D78 (= D75), S100 (= S97), T101 (= T98), F237 (= F234), Y238 (≠ P235), Y241 (≠ L238), K244 (= K241), D245 (= D242)

3w6zA Crystal structure of NADP bound l-serine 3-dehydrogenase (k170m) from hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
33% identity, 94% coverage: 5:276/290 of query aligns to 16:286/296 of 3w6zA

• binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G9), L21 (= L10), G22 (= G11), I23 (= I12), M24 (= M13), N43 (= N33), R44 (= R34), T45 (≠ S35), K48 (≠ A38), M76 (= M66), V77 (≠ L67), S78 (≠ A68), D82 (≠ V72), Q85 (≠ D75), V86 (≠ M76), S107 (= S97), T108 (= T98), V133 (= V123), G136 (≠ T126), M183 (≠ K173), G243 (≠ Q233), F244 (= F234), K245 (≠ P235), H248 (≠ L238), K251 (= K241), D252 (= D242)

Q49A26 Cytokine-like nuclear factor N-PAC; NPAC; 3-hydroxyisobutyrate dehydrogenase-like protein; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Nuclear protein of 60 kDa; Nucleosome-destabilizing factor; hNDF; Putative oxidoreductase GLYR1 from Homo sapiens (Human) (see 3 papers)
29% identity, 98% coverage: 3:285/290 of query aligns to 268:549/553 of Q49A26

• 271:285 (vs. 6:20, 53% identical) binding
• T362 (= T98) binding
• M437 (≠ K173) mutation to K: Loss of tetramerization and protein stability.; mutation to N: No effect on tetramerization or protein stability.
• P496 (≠ A232) to L: decreased interaction with GATA4; decreased synergistic activation of GATA4 target genes transcription; detrimental effect on cardiomyocyte differentiation
• K505 (= K241) binding

Sites not aligning to the query:

• 214 D→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
• 214:217 Interaction with histone H3
• 216 H→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
• 216:225 Interaction with KDM1B
• 217 Required to promote KDM1B demethylase activity toward histone H3K4me1 and H3K4me2; F→A: Abolished stimulation of KDM1B demethylase activity, reduced affinity for histone H3 of the dimer with KDM1B, but normal KDM1B-binding.
• 219 H→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-223.
• 220:222 FLL→AAA: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity.
• 223 S→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-219.

Q922P9 Cytokine-like nuclear factor N-PAC; NPAC; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Putative oxidoreductase GLYR1 from Mus musculus (Mouse) (see paper)
29% identity, 98% coverage: 3:285/290 of query aligns to 267:542/546 of Q922P9

• P489 (≠ A232) mutation to L: Mutant animals are born at expected Mendelian ratios. 54% mutants display postnatal lethality between days 0 and 1. They show centricular septal defects.

5y8oB Mycobacterium tuberculosis 3-hydroxyisobutyrate dehydrogenase (mthibadh) + NAD + 3-hydroxy propionate (3-hp) (see paper)
33% identity, 99% coverage: 1:287/290 of query aligns to 1:289/291 of 5y8oB