SitesBLAST
Comparing GFF138 FitnessBrowser__psRCH2:GFF138 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3tqlA Structure of the amino acid abc transporter, periplasmic amino acid- binding protein from coxiella burnetii. (see paper)
41% identity, 89% coverage: 24:246/251 of query aligns to 1:225/225 of 3tqlA
1hslA Refined 1.89 angstroms structure of the histidine-binding protein complexed with histidine and its relationship with many other active transport(slash)chemosensory receptors (see paper)
38% identity, 90% coverage: 25:251/251 of query aligns to 4:236/238 of 1hslA
P02910 Histidine-binding periplasmic protein; HBP from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
38% identity, 90% coverage: 25:251/251 of query aligns to 26:258/260 of P02910
- E40 (≠ N39) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-47.
- K42 (≠ I41) mutation to E: Increases ATPase-inducing activity and histidine transport; when associated with K-47.
- E47 (≠ K46) mutation to K: Decrease in ATPase-inducing activity and histidine transport; when associated with K-40. Increases ATPase-inducing activity and histidine transport; when associated with E-42.
- D171 (= D169) mutation D->A,N: Strong decrease in ATPase-inducing activity and histidine transport.
- R176 (= R174) mutation R->D,S: Strong decrease in ATPase-inducing activity and histidine transport.
- D178 (= D176) mutation to A: Slight decrease in ATPase-inducing activity and histidine transport.
Sites not aligning to the query:
4zv2A An ancestral arginine-binding protein bound to glutamine (see paper)
41% identity, 88% coverage: 27:246/251 of query aligns to 6:223/225 of 4zv2A
4zv1A An ancestral arginine-binding protein bound to arginine (see paper)
41% identity, 88% coverage: 27:246/251 of query aligns to 6:225/226 of 4zv1A
- binding arginine: E11 (= E32), F14 (≠ Y35), F52 (≠ W73), A69 (= A90), G70 (≠ S91), M71 (= M92), T72 (≠ S93), R77 (= R98), Q117 (= Q138), S120 (≠ T145), T121 (≠ W146), D161 (= D181)
P02911 Lysine/arginine/ornithine-binding periplasmic protein; LAO-binding protein from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
37% identity, 98% coverage: 5:251/251 of query aligns to 2:258/260 of P02911
- D33 (≠ E32) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- Y36 (= Y35) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- D52 (= D51) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- C60 (= C59) modified: Disulfide link with 67
- C67 (= C66) modified: Disulfide link with 60
- F74 (≠ W73) mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- S91 (≠ A90) binding L-arginine; binding L-ornithine; mutation to A: Has no effect on the arginine and histidine binding affinity.
- S92 (= S91) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- S94 (= S93) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Decreases arginine and histidine binding affinity.
- R99 (= R98) binding L-arginine; binding L-lysine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
- L139 (≠ Q138) mutation to K: Changes ligand specificity. Can bind glutamine. Still able to bind basic amino acids, however, with 1000-fold less affinity for arginine.; mutation to Q: Does not affect ligand preference.
- T143 (≠ I142) binding L-arginine; binding L-lysine; binding L-ornithine
- D183 (= D181) binding L-arginine; binding L-ornithine; mutation to A: Shows a drastic decrease in arginine and histidine binding affinity.
P0AEU0 Histidine-binding periplasmic protein; HBP from Escherichia coli (strain K12) (see 3 papers)
37% identity, 90% coverage: 25:251/251 of query aligns to 26:258/260 of P0AEU0
- C60 (= C59) modified: Disulfide link with 67
- C67 (= C66) modified: Disulfide link with 60
- S91 (≠ A90) binding L-histidine
- S92 (= S91) binding L-histidine
- S94 (= S93) binding L-histidine
- R99 (= R98) binding L-histidine
- T143 (≠ I142) binding L-histidine
- D183 (= D181) binding L-histidine
Sites not aligning to the query:
5owfA Structure of a lao-binding protein mutant with glutamine (see paper)
36% identity, 90% coverage: 25:251/251 of query aligns to 1:233/235 of 5owfA
1lstA Three-dimensional structures of the periplasmic lysine-, arginine-, ornithine-binding protein with and without a ligand (see paper)
36% identity, 90% coverage: 25:251/251 of query aligns to 4:236/238 of 1lstA
1lahE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
36% identity, 90% coverage: 25:251/251 of query aligns to 4:236/238 of 1lahE
1lagE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
36% identity, 90% coverage: 25:251/251 of query aligns to 4:236/238 of 1lagE
- binding histidine: Y14 (= Y35), F52 (≠ W73), S69 (≠ A90), S70 (= S91), L71 (≠ M92), S72 (= S93), R77 (= R98), L117 (≠ Q138), S120 (≠ T141), Q122 (≠ A143), D161 (= D181)
1lafE Structural bases for multiple ligand specificity of the periplasmic lysine-, arginine-, ornithine-binding protein (see paper)
36% identity, 90% coverage: 25:251/251 of query aligns to 4:236/238 of 1lafE
- binding arginine: D11 (≠ E32), Y14 (= Y35), F52 (≠ W73), S69 (≠ A90), S70 (= S91), S72 (= S93), R77 (= R98), L117 (≠ Q138), S120 (≠ T141), T121 (≠ I142), Q122 (≠ A143), D161 (= D181)
3vvfA Crystal structure of ttc0807 complexed with arginine (see paper)
37% identity, 88% coverage: 26:246/251 of query aligns to 18:234/241 of 3vvfA
- binding arginine: E24 (= E32), F27 (≠ Y35), F65 (≠ W73), A82 (= A90), S83 (= S91), H84 (≠ M92), G85 (≠ S93), R90 (= R98), Q128 (= Q138), T131 (= T141), T132 (= T145), Y133 (≠ W146), E196 (≠ D208)
3vveA Crystal structure of ttc0807 complexed with lysine (see paper)
37% identity, 88% coverage: 26:246/251 of query aligns to 18:234/241 of 3vveA
- binding lysine: E24 (= E32), F27 (≠ Y35), F65 (≠ W73), S83 (= S91), H84 (≠ M92), R90 (= R98), Q128 (= Q138), T131 (= T141), T132 (= T145), Y133 (≠ W146), E196 (≠ D208)
3vvdA Crystal structure of ttc0807 complexed with ornithine (see paper)
37% identity, 88% coverage: 26:246/251 of query aligns to 18:234/241 of 3vvdA
- binding L-ornithine: E24 (= E32), F65 (≠ W73), S83 (= S91), H84 (≠ M92), G85 (≠ S93), R90 (= R98), Q128 (= Q138), T131 (= T141), T132 (= T145), Y133 (≠ W146), E196 (≠ D208)
3vv5A Crystal structure of ttc0807 complexed with (s)-2-aminoethyl-l- cysteine (aec) (see paper)
37% identity, 88% coverage: 26:246/251 of query aligns to 14:230/237 of 3vv5A
- binding l-thialysine: E20 (= E32), F23 (≠ Y35), N27 (= N39), F61 (≠ W73), A78 (= A90), S79 (= S91), G81 (≠ S93), R86 (= R98), T127 (= T141), T128 (= T145), Y129 (≠ W146)
2y7iA Structural basis for high arginine specificity in salmonella typhimurium periplasmic binding protein stm4351. (see paper)
32% identity, 90% coverage: 21:246/251 of query aligns to 2:227/228 of 2y7iA
- binding arginine: Y16 (= Y35), E20 (≠ N39), F54 (≠ W73), A71 (= A90), G72 (≠ S91), D74 (≠ S93), R79 (= R98), E118 (≠ Q138), T121 (= T141), T122 (≠ I142), D160 (= D181)
- binding zinc ion: H9 (≠ R28), D23 (= D42), D25 (≠ S44), D55 (= D74), D67 (= D86), D140 (= D161)
8hqrA Crystal structure of the arginine-/lysine-binding protein sar11_1210 from 'candidatus pelagibacter ubique' htcc1062 bound to arginine (see paper)
33% identity, 89% coverage: 25:248/251 of query aligns to 8:263/267 of 8hqrA
- binding arginine: E15 (= E32), Y18 (= Y35), W56 (= W73), A73 (= A90), G74 (≠ S91), S76 (= S93), R81 (= R98), E94 (≠ N111), Q143 (= Q138), T146 (= T141), I147 (= I142)
5t0wA Crystal structure of the ancestral amino acid-binding protein anccdt- 1, a precursor of cyclohexadienyl dehydratase
33% identity, 88% coverage: 27:246/251 of query aligns to 12:228/229 of 5t0wA