SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1457 HP15_1422 amino acid ABC transporter ATP-binding protein to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
59% identity, 100% coverage: 1:239/240 of query aligns to 1:239/240 of 4ymuJ

query
sites
4ymuJ

M

M

I

I

S

F

V

V

Q

N

N

D

V

V

H

Y

K

K

S

N

F
|
F

G

G

D

S

L
|
L

E

E

V
|
V

L

L

K

K

G

G

V

V

S

T

L

L

D

K

V

V

K

N

K

K

G

G

E

E

V

V

V

V

S

V

V

I

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

Y

R

C

C

I

I

N

N

A

L

I

L

E
|
E

P

E

I

P

N

T

K

K

G

G

K

E

I

V

L

F

V

I

D

D

D

G

V

V

D

K

V

I

H

N

A

N

K

G

E

K

T

V

N

N

K

I

D

N

K

K

L

V

R

R

Q

Q

K

K

L

V

G

G

M

M

V

V

F

F

Q

Q

Q

H

W

F

N

N

S

L

F

F

P

P

H

H

M

L

T

T

V

A

L

I

E

E

N

N

A

I

A

T

L

L

A

A

P

P

R

V

I

K

V

V

K

K

K

K

M

M

S

N

K

K

E

K

E

E

A

A

I

E

E

E

I

L

A

A

K

V

K

D

E

L

L

L

E

A

H

K

V

V

G

G

L

L

G

L

D

D

K

K

F

K

D

D

V

Q

Y

Y

P

P

T

I

K

K

M

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

L

L

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

K

Q

P

P

D

E

Y

V

M

M

L

L

L

F

D

D

E
|
E

V

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

K

E

E

V

V

L

L

D

N

T

V

L

M

R

K

L

Q

L

L

A

A

E

N

E

E

G

G

M

M

T

T

M

M

I

V

C

V

V

V

T

T

H
|
H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

A

I

Y

F

F

M

H

D

E

D

G

G

V

V

I

I

A

V

E

E

I

E

G

G

E

T

A

P

R

E

Q

E

V

I

I

F

T

Y

D

R

P

A

Q

K

N

N

P

E

L

R

T

T

Q

R

K

E

F

F

L

L

S

S

K

K

V

I

binding adenosine-5'-triphosphate: F11 (= F11), L14 (= L14), V16 (= V16), S36 (= S36), G37 (= G37), S38 (= S38), G39 (= G39), K40 (= K40), S41 (= S41), T42 (= T42), E51 (= E51), E162 (= E162), H194 (= H194)
binding magnesium ion: S41 (= S41), E162 (= E162)

3c4jA Abc protein artp in complex with atp-gamma-s
56% identity, 100% coverage: 1:239/240 of query aligns to 3:241/242 of 3c4jA

query
sites
3c4jA

M

M

I

I

S

D

V

V

Q

H

N

Q

V

L

H

K

K

K

S

S

F
|
F

G

G

D

S

L

L

E

E

V
|
V

L

L

K

K

G

G

V

I

S

N

L

V

D

H

V

I

K

R

K

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

G
x
P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

Y

R

C

C

I

L

N

N

A

L

I

L

E

E

P

D

I

F

N

D

K

E

G

G

K

E

I

I

L

I

V

I

D

D

D

G

V

I

D

N

V

L

H

K

A

A

K

K

E

D

T

T

N

N

K

L

D

N

K

K

L

V

R

R

Q

E

K

E

L

V

G

G

M

M

V

V

F

F

Q

Q

Q

R

W

F

N

N

S

L

F

F

P

P

H

H

M

M

T

T

V

V

L

L

E

N

N

N

A

I

A

T

L

L

A

A

P

P

R

M

I

K

V

V

K

R

K

K

M

W

S

P

K

R

E

E

E

K

A

A

I

E

E

A

I

K

A

A

K

M

K

E

E

L

L

L

E

D

H

K

V

V

G

G

L

L

G

K

D

D

K

K

F

A

D
x
H

V

A

Y
|
Y

P

P

T

D

K

S

M

L

S

S

G

G

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

K

E

P

P

D

K

Y

I

M

M

L

L

L

F

D

D

E
|
E

V

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

G

E

E

V

V

L

L

D

S

T

V

L

M

R

K

L

Q

L

L

A

A

E

N

E

E

G

G

M

M

T

T

M

M

I

V

C

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

A

L

Y

F

F

M

H

D

E

G

G

G

V

Y

I

I

A

I

E

E

I

E

G

G

E

K

A

P

R

E

Q

D

V

L

I

F

T

D

D

R

P

P

Q

Q

N

H

P

E

L

R

T

T

Q

K

K

A

F

F

L

L

S

S

K

K

V

V

binding phosphothiophosphoric acid-adenylate ester: F13 (= F11), V18 (= V16), P37 (≠ G35), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), H133 (≠ D131), Y135 (= Y133), E164 (= E162)

3c41J Abc protein artp in complex with amp-pnp/mg2+
56% identity, 100% coverage: 1:239/240 of query aligns to 3:241/242 of 3c41J

query
sites
3c41J

M

M

I

I

S

D

V

V

Q

H

N

Q

V

L

H

K

K

K

S

S

F
|
F

G

G

D

S

L

L

E

E

V
|
V

L

L

K

K

G

G

V

I

S

N

L

V

D

H

V

I

K

R

K

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

G
x
P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

Y
x
R

C

C

I

L

N

N

A

L

I

L

E

E

P

D

I

F

N

D

K

E

G

G

K

E

I

I

L

I

V

I

D

D

D

G

V

I

D

N

V

L

H

K

A

A

K

K

E

D

T

T

N

N

K

L

D

N

K

K

L

V

R

R

Q

E

K

E

L

V

G

G

M

M

V

V

F

F

Q

Q

Q

R

W

F

N

N

S

L

F

F

P

P

H

H

M

M

T

T

V

V

L

L

E

N

N

N

A

I

A

T

L

L

A

A

P

P

R

M

I

K

V

V

K

R

K

K

M

W

S

P

K

R

E

E

E

K

A

A

I

E

E

A

I

K

A

A

K

M

K

E

E

L

L

L

E

D

H

K

V

V

G

G

L

L

G

K

D

D

K

K

F

A

D
x
H

V

A

Y
|
Y

P

P

T

D

K

S

M

L

S

S

G

G

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

K

E

P

P

D

K

Y

I

M

M

L

L

L

F

D
|
D

E

E

V

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

G

E

E

V

V

L

L

D

S

T

V

L

M

R

K

L

Q

L

L

A

A

E

N

E

E

G

G

M

M

T

T

M

M

I

V

C

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

A

L

Y

F

F

M

H

D

E

G

G

G

V

Y

I

I

A

I

E

E

I

E

G

G

E

K

A

P

R

E

Q

D

V

L

I

F

T

D

D

R

P

P

Q

Q

N

H

P

E

L

R

T

T

Q

K

K

A

F

F

L

L

S

S

K

K

V

V

binding phosphoaminophosphonic acid-adenylate ester: F13 (= F11), V18 (= V16), P37 (≠ G35), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), R47 (≠ Y45), H133 (≠ D131), Y135 (= Y133)
binding magnesium ion: S43 (= S41), D163 (= D161)

2olkA Abc protein artp in complex with adp-beta-s
56% identity, 100% coverage: 1:239/240 of query aligns to 3:241/242 of 2olkA

query
sites
2olkA

M

M

I

I

S

D

V

V

Q

H

N

Q

V

L

H

K

K

K

S

S

F
|
F

G

G

D

S

L
|
L

E

E

V
|
V

L

L

K

K

G

G

V

I

S

N

L

V

D

H

V

I

K

R

K

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

Y

R

C

C

I

L

N

N

A

L

I

L

E

E

P

D

I

F

N

D

K

E

G

G

K

E

I

I

L

I

V

I

D

D

D

G

V

I

D

N

V

L

H

K

A

A

K

K

E

D

T

T

N

N

K

L

D

N

K

K

L

V

R

R

Q

E

K

E

L

V

G

G

M

M

V

V

F

F

Q

Q

Q

R

W

F

N

N

S

L

F

F

P

P

H

H

M

M

T

T

V

V

L

L

E

N

N

N

A

I

A

T

L

L

A

A

P

P

R

M

I

K

V

V

K

R

K

K

M

W

S

P

K

R

E

E

E

K

A

A

I

E

E

A

I

K

A

A

K

M

K

E

E

L

L

L

E

D

H

K

V

V

G

G

L

L

G

K

D

D

K

K

F

A

D

H

V

A

Y

Y

P

P

T

D

K

S

M

L

S

S

G

G

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

K

E

P

P

D

K

Y

I

M

M

L

L

L

F

D

D

E

E

V

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

G

E

E

V

V

L

L

D

S

T

V

L

M

R

K

L

Q

L

L

A

A

E

N

E

E

G

G

M

M

T

T

M

M

I

V

C

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

A

L

Y

F

F

M

H

D

E

G

G

G

V

Y

I

I

A

I

E

E

I

E

G

G

E

K

A

P

R

E

Q

D

V

L

I

F

T

D

D

R

P

P

Q

Q

N

H

P

E

L

R

T

T

Q

K

K

A

F

F

L

L

S

S

K

K

V

V

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: F13 (= F11), L16 (= L14), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42)

2oljA Abc protein artp in complex with adp/mg2+
56% identity, 100% coverage: 1:239/240 of query aligns to 3:241/242 of 2oljA

query
sites
2oljA

M

M

I

I

S

D

V

V

Q

H

N

Q

V

L

H

K

K

K

S

S

F
|
F

G

G

D

S

L

L

E

E

V
|
V

L

L

K

K

G

G

V

I

S

N

L

V

D

H

V

I

K

R

K

E

G

G

E

E

V

V

V

V

S

V

V

V

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

Y

R

C

C

I

L

N

N

A

L

I

L

E

E

P

D

I

F

N

D

K

E

G

G

K

E

I

I

L

I

V

I

D

D

D

G

V

I

D

N

V

L

H

K

A

A

K

K

E

D

T

T

N

N

K

L

D

N

K

K

L

V

R

R

Q

E

K

E

L

V

G

G

M

M

V

V

F

F

Q

Q

Q

R

W

F

N

N

S

L

F

F

P

P

H

H

M

M

T

T

V

V

L

L

E

N

N

N

A

I

A

T

L

L

A

A

P

P

R

M

I

K

V

V

K

R

K

K

M

W

S

P

K

R

E

E

E

K

A

A

I

E

E

A

I

K

A

A

K

M

K

E

E

L

L

L

E

D

H

K

V

V

G

G

L

L

G

K

D

D

K

K

F

A

D
x
H

V

A

Y
|
Y

P

P

T

D

K

S

M

L

S

S

G

G

G

G

Q

Q

Q

A

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

K

E

P

P

D

K

Y

I

M

M

L

L

L

F

D

D

E

E

V

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

G

E

E

V

V

L

L

D

S

T

V

L

M

R

K

L

Q

L

L

A

A

E

N

E

E

G

G

M

M

T

T

M

M

I

V

C

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

G

D

D

R

R

V

V

A

L

Y

F

F

M

H

D

E

G

G

G

V

Y

I

I

A

I

E

E

I

E

G

G

E

K

A

P

R

E

Q

D

V

L

I

F

T

D

D

R

P

P

Q

Q

N

H

P

E

L

R

T

T

Q

K

K

A

F

F

L

L

S

S

K

K

V

V

binding adenosine-5'-diphosphate: F13 (= F11), V18 (= V16), S38 (= S36), G39 (= G37), S40 (= S38), G41 (= G39), K42 (= K40), S43 (= S41), T44 (= T42), H133 (≠ D131), Y135 (= Y133)

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
53% identity, 100% coverage: 1:239/240 of query aligns to 2:239/241 of 4u00A

query
sites
4u00A

M

I

I

I

S

R

V

I

Q

R

N

N

V

L

H

H

K

K

S

W

F
|
F

G

G

D

P

L
|
L

E

H

V
|
V

L

L

K

K

G

G

V

I

S

H

L

L

D

E

V

V

K

A

K

P

G

G

E

E

V

K

V

L

S

V

V

I

I

I

G

G

G

P

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

Y

R

C

T

I

I

N

N

A

R

I

L

E

E

P

D

I

F

N

Q

K

E

G

G

K

E

I

V

L

V

V

V

D

D

D

G

V

L

D

S

V

V

H

K

A

D

K

D

E

R

T

A

N

L

K

R

D

E

K

-

L

I

R

R

Q

R

K

E

L

V

G

G

M

M

V

V

F

F

Q

Q

Q

Q

W

F

N

N

S

L

F

F

P

P

H

H

M

M

T

T

V

V

L

L

E

E

N

N

A

V

A

T

L

L

A

A

P

P

R

M

I

R

V

V

K

R

K

R

M

W

S

P

K

R

E

E

E

K

A

A

I

E

E

K

I

K

A

A

K

L

K

E

E

L

L

L

E

E

H

R

V

V

G

G

L

I

G

L

D

D

K

Q

F

A

D

R

V

K

Y

Y

P

P

T

A

K

Q

M

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

M

K

E

P

P

D

K

Y

I

M

M

L

L

L

F

D

D

E

E

V

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

M

V

V

G

G

E

E

V

V

L

L

D

D

T

V

L

M

R

R

L

D

L

L

A

A

E

Q

E

G

G

G

M

M

T

T

M

M

I

V

C

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

E

V

V

S

A

D

D

R

R

V

V

A

V

Y

F

F

M

H

D

E

G

G

G

V

Q

I

I

A

V

E

E

I

E

G

G

E

R

A

P

R

E

Q

E

V

I

I

F

T

T

D

R

P

P

Q

K

N

E

P

E

L

R

T

T

Q

R

K

S

F

F

L

L

S

Q

K

R

V

V

binding adenosine-5'-diphosphate: F12 (= F11), L15 (= L14), V17 (= V16), S37 (= S36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (= T42)

1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
52% identity, 98% coverage: 2:236/240 of query aligns to 3:249/258 of 1b0uA

query
sites
1b0uA

I

L

S

H

V

V

Q

I

N

D

V

L

H

H

K

K

S

R

F
x
Y

G

G

D

G

L
x
H

E

E

V
|
V

L

L

K

K

G

G

V

V

S

S

L

L

D

Q

V

A

K

R

K

A

G

G

E

D

V

V

V

I

S

S

V

I

I

I

G

G

G

S

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

F

L

L

Y

R

C

C

I

I

N

N

A

F

I

L

E

E

P

K

I

P

N

S

K

E

G

G

K

A

I

I

L

I

V

V

D

N

-

G

-

Q

-

N

-

I

-

N

-

L

-

V

-

R

D

D

V

K

D

D

V

G

H

Q

A

L

K

K

E

V

T

A

N

D

K

K

D

N

K

Q

L

L

R

R

-

L

-

L

-

R

Q

T

K

R

L

L

G

T

M

M

V

V

F

F

Q

Q

Q

H

W

F

N

N

S

L

F

W

P

S

H

H

M

M

T

T

V

V

L

L

E

E

N

N

A

V

A

M

L

E

A

A

P

P

R

I

I

Q

V

V

K

L

K

G

M

L

S

S

K

K

E

H

E

D

A

A

I

R

E

E

I

R

A

A

K

L

K

K

E

Y

L

L

E

A

H

K

V

V

G

G

L

I

G

D

D

E

K

R

F

A

D

Q

-

G

V

K

Y

Y

P

P

T

V

K

H

M

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

L

V

A

S

I

I

A

A

R

R

A

A

L

L

A

A

M

M

K

E

P

P

D

D

Y

V

M

L

L

L

L

F

D

D

E

E

V

P

T

T

S

S

A

A

L

L

D

D

P

P

E

E

L

L

V

V

G

G

E

E

V

V

L

L

D

R

T

I

L

M

R

Q

L

Q

L

L

A

A

E

E

E

E

G

G

M

K

T

T

M

M

I

V

C

V

V

V

T

T

H

H

E

E

M

M

G

G

F

F

A

A

R

R

D

H

V

V

S

S

D

S

R

H

V

V

A

I

Y

F

F

L

H

H

E

Q

G

G

V

K

I

I

A

E

E

E

I

E

G

G

E

D

A

P

R

E

Q

Q

V

V

I

F

T

G

D

N

P

P

Q

Q

N

S

P

P

L

R

T

L

Q

Q

K

Q

F

F

L

L

binding adenosine-5'-triphosphate: Y12 (≠ F11), H15 (≠ L14), V17 (= V16), S37 (= S36), G38 (= G37), S39 (= S38), G40 (= G39), K41 (= K40), S42 (= S41), T43 (= T42)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
40% identity, 98% coverage: 1:236/240 of query aligns to 1:241/343 of P30750

query
sites
P30750

M

M

I

I

S

K

V

L

Q

S

N

N

V

I

H

T

K

K

S

V

F

F

G

H

D

Q

-

G

-

T

-

R

-

T

L

I

E

Q

V

A

L

L

K

N

G

N

V

V

S

S

L

L

D

H

V

V

K

P

K

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

G

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

Y

R

C

C

I

V

N

N

A

L

I

L

E

E

P

R

I

P

N

T

K

E

G

G

K

S

I

V

L

L

V

V

D

D

D

G

V

Q

D

E

V

L

H

T

A

T

-

L

K

S

E

E

T

S

N

E

K

L

D

T

K

K

L

A

R

R

Q

R

K

Q

L

I

G

G

M

M

V

I

F

F

Q

Q

Q

H

W

F

N

N

S

L

F

L

P

S

H

S

M

R

T

T

V

V

L

F

E

G

N

N

A

V

A

A

L

L

A

-

P

P

R

L

I

E

V

L

K

D

K

N

M

T

S

P

K

K

E

D

E

E

A

V

I

K

E

R

I

R

A

V

K

T

K

E

E

L

L

L

E

S

H

L

V

V

G

G

L

L

G

G

D

D

K

K

F

H

D

D

V

S

Y

Y

P

P

T

S

K

N

M

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

K

N

P

P

D

K

Y

V

M

L

L

L

L

C

D

D

E
|
E

V

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

D

E

T

L

L

L

R

K

L

D

L

I

A

N

E

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

C

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

A

A

Y

V

F

I

H

S

E

N

G

G

V

E

I

L

A

I

E

E

I

Q

G

D

E

T

A

V

R

S

Q

E

V

V

I

F

T

S

D

H

P

P

Q

K

N

T

P

P

L

L

T

A

Q

Q

K

K

F

F

L

I

40:46 (vs. 36:42, 86% identical) binding
E166 (= E162) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
40% identity, 98% coverage: 1:236/240 of query aligns to 2:242/344 of 6cvlD

query
sites
6cvlD

M

M

I

I

S

K

V

L

Q

S

N

N

V

I

H

T

K

K

S

V

F
|
F

G

H

D
x
Q

-

G

-

T

-

R

-

T

L
x
I

E

Q

V

A

L

L

K

N

G

N

V

V

S

S

L

L

D

H

V

V

K

P

K

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

G

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

Y

R

C

C

I

V

N

N

A

L

I

L

E

E

P

R

I

P

N

T

K

E

G

G

K

S

I

V

L

L

V

V

D

D

D

G

V

Q

D

E

V

L

H

T

A

T

-

L

K

S

E

E

T

S

N

E

K

L

D

T

K

K

L

A

R

R

Q

R

K

Q

L

I

G

G

M

M

V

I

F

F

Q

Q

Q

H

W

F

N

N

S

L

F

L

P

S

H

S

M

R

T

T

V

V

L

F

E

G

N

N

A

V

A

A

L

L

A

-

P

P

R

L

I

E

V

L

K

D

K

N

M

T

S

P

K

K

E

D

E

E

A

V

I

K

E

R

I

R

A

V

K

T

K

E

E

L

L

L

E

S

H

L

V

V

G

G

L

L

G

G

D

D

K

K

F

H

D

D

V

S

Y

Y

P

P

T

S

K
x
N

M
x
L

S
|
S

G
|
G

G
|
G

Q
|
Q

Q

K

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

K

N

P

P

D

K

Y

V

M

L

L

L

L

C

D

D

E

Q

V

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

D

E

T

L

L

L

R

K

L

D

L

I

A

N

E

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

C

L

V

I

T

T

H
|
H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

A

A

Y

V

F

I

H

S

E

N

G

G

V

E

I

L

A

I

E

E

I

Q

G

D

E

T

A

V

R

S

Q

E

V

V

I

F

T

S

D

H

P

P

Q

K

N

T

P

P

L

L

T

A

Q

Q

K

K

F

F

L

I

binding phosphothiophosphoric acid-adenylate ester: F12 (= F11), Q14 (≠ D13), I19 (≠ L14), S41 (= S36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42), N141 (≠ K136), L142 (≠ M137), S143 (= S138), G144 (= G139), G145 (= G140), Q146 (= Q141), H200 (= H194)

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
40% identity, 98% coverage: 1:236/240 of query aligns to 2:242/344 of 3tuzC

query
sites
3tuzC

M

M

I

I

S

K

V

L

Q

S

N

N

V

I

H

T

K

K

S

V

F
|
F

G

H

D
x
Q

-

G

-

T

-

R

-

T

L

I

E

Q

V
x
A

L

L

K

N

G

N

V

V

S

S

L

L

D

H

V

V

K

P

K

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

G

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

Y

R

C

C

I

V

N

N

A

L

I

L

E

E

P

R

I

P

N

T

K

E

G

G

K

S

I

V

L

L

V

V

D

D

D

G

V

Q

D

E

V

L

H

T

A

T

-

L

K

S

E

E

T

S

N

E

K

L

D

T

K

K

L

A

R

R

Q

R

K

Q

L

I

G

G

M

M

V

I

F

F

Q

Q

Q

H

W

F

N

N

S

L

F

L

P

S

H

S

M

R

T

T

V

V

L

F

E

G

N

N

A

V

A

A

L

L

A

-

P

P

R

L

I

E

V

L

K

D

K

N

M

T

S

P

K

K

E

D

E

E

A

V

I

K

E

R

I

R

A

V

K

T

K

E

E

L

L

L

E

S

H

L

V

V

G

G

L

L

G

G

D

D

K

K

F

H

D

D

V

S

Y

Y

P

P

T

S

K

N

M

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

K

N

P

P

D

K

Y

V

M

L

L

L

L

C

D

D

E

Q

V

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

D

E

T

L

L

L

R

K

L

D

L

I

A

N

E

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

C

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

A

A

Y

V

F

I

H

S

E

N

G

G

V

E

I

L

A

I

E

E

I

Q

G

D

E

T

A

V

R

S

Q

E

V

V

I

F

T

S

D

H

P

P

Q

K

N

T

P

P

L

L

T

A

Q

Q

K

K

F

F

L

I

binding adenosine-5'-diphosphate: F12 (= F11), Q14 (≠ D13), A21 (≠ V16), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
40% identity, 98% coverage: 1:236/240 of query aligns to 2:242/344 of 3tuiC

query
sites
3tuiC

M

M

I

I

S

K

V

L

Q

S

N

N

V

I

H

T

K

K

S

V

F
|
F

G

H

D
x
Q

-

G

-

T

-

R

-

T

L

I

E

Q

V

A

L

L

K

N

G

N

V

V

S

S

L

L

D

H

V

V

K

P

K

A

G

G

E

Q

V

I

V

Y

S

G

V

V

I

I

G

G

G

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

I

Y

R

C

C

I

V

N

N

A

L

I

L

E

E

P

R

I

P

N

T

K

E

G

G

K

S

I

V

L

L

V

V

D

D

D

G

V

Q

D

E

V

L

H

T

A

T

-

L

K

S

E

E

T

S

N

E

K

L

D

T

K

K

L

A

R

R

Q

R

K

Q

L

I

G

G

M

M

V

I

F

F

Q

Q

Q

H

W

F

N

N

S

L

F

L

P

S

H

S

M

R

T

T

V

V

L

F

E

G

N

N

A

V

A

A

L

L

A

-

P

P

R

L

I

E

V

L

K

D

K

N

M

T

S

P

K

K

E

D

E

E

A

V

I

K

E

R

I

R

A

V

K

T

K

E

E

L

L

L

E

S

H

L

V

V

G

G

L

L

G

G

D

D

K

K

F

H

D

D

V

S

Y

Y

P

P

T

S

K

N

M

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

L

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

M

S

K

N

P

P

D

K

Y

V

M

L

L

L

L

C

D

D

E

Q

V

A

T

T

S

S

A

A

L

L

D

D

P

P

E

A

L

T

V

T

G

R

E

S

V

I

L

L

D

E

T

L

L

L

R

K

L

D

L

I

A

N

E

R

E

R

-

L

G

G

M

L

T

T

M

I

I

L

C

L

V

I

T

T

H

H

E

E

M

M

G

D

F

V

A

V

R

K

D

R

V

I

S

C

D

D

R

C

V

V

A

A

Y

V

F

I

H

S

E

N

G

G

V

E

I

L

A

I

E

E

I

Q

G

D

E

T

A

V

R

S

Q

E

V

V

I

F

T

S

D

H

P

P

Q

K

N

T

P

P

L

L

T

A

Q

Q

K

K

F

F

L

I

binding adenosine-5'-diphosphate: F12 (= F11), Q14 (≠ D13), S41 (= S36), G42 (= G37), A43 (≠ S38), G44 (= G39), K45 (= K40), S46 (= S41), T47 (= T42)

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
41% identity, 90% coverage: 21:236/240 of query aligns to 46:263/382 of 7ahhC

query
sites
7ahhC

S

N

L

F

D

E

V

I

K

N

K

E

G

G

E

E

V

I

V

F

S

V

V

I

I

M

G

G

G

L

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

Y

R

C

L

I

L

N

N

A

R

-

L

I

I

E

E

P

P

I

T

N

S

K

-

G

G

K

K

I

I

L

F

V

I

D

D

D

N

V

Q

D

D

V

V

H

A

A

T

K

-

E

-

T

L

N

N

K

K

D

E

K

D

L

L

-

L

-

Q

-

V

-

R

R

R

Q

K

K

T

L

M

G

S

M

M

V

V

F

F

Q

Q

Q

N

W

F

N

G

S

L

F

F

P

P

H

H

M

R

T

T

V

I

L

L

E

E

N

N

A

T

A

E

L

Y

A

G

P

L

R

E

I

-

V

V

K

Q

K

N

M

V

S

P

K

K

E

E

E

E

A

R

I

R

E

K

I

R

A

A

K

E

K

K

E

A

L

L

E

D

H

N

V

A

G

N

L

L

G

L

D

D

K

F

F

K

D

D

V

Q

Y

Y

P

P

T

K

K

Q

M

L

S

S

G

G

G

G

Q

M

Q

Q

Q

Q

R

R

L

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

M

N

K

D

P

P

D

E

Y

I

M

L

L

L

L

M

D
|
D

E
|
E

V

A

T

F

S

S

A

A

L

L

D

D

P

P

E

L

L

I

V

R

G

R

E

E

V

M

L

Q

D

D

T

E

-

L

L

L

R

E

L

L

L

Q

A

A

E

K

E

F

G

Q

M

K

T

T

M

I

I

I

C

F

V

V

T

S

H

H

E

D

M

L

G

N

F

E

A

A

R

L

D

R

V

I

S

G

D

D

R

R

V

I

A

A

Y

I

F

M

H

K

E

D

G

G

V

K

I

I

A

M

E

Q

I

I

G

G

E

T

A

G

R

E

Q

E

V

I

I

L

T

T

D

N

P

P

Q

A

N

N

P

D

L

Y

T

V

Q

K

K

T

F

F

L

V

binding phosphoaminophosphonic acid-adenylate ester: S61 (= S36), G62 (= G37), S63 (= S38), G64 (= G39), K65 (= K40), S66 (= S41), T67 (= T42), D187 (= D161), E188 (= E162)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
41% identity, 90% coverage: 21:236/240 of query aligns to 46:263/382 of 7aheC

query
sites
7aheC

S

N

L

F

D

E

V

I

K

N

K

E

G

G

E

E

V

I

V

F

S

V

V

I

I

M

G

G

G

L

S

S

G

G

S

S

G

G

K

K

S

S

T

T

L

L

L

L

Y

R

C

L

I

L

N

N

A

R

-

L

I

I

E

E

P

P

I

T

N

S

K

-

G

G

K

K

I

I

L

F

V

I

D

D

D

N

V

Q

D

D

V

V

H

A

A

T

K

-

E

-

T

L

N

N

K

K

D

E

K

D

L

L

-

L

-

Q

-

V

-

R

R

R

Q

K

K

T

L

M

G

S

M

M

V

V

F

F

Q

Q

Q

N

W

F

N

G

S

L

F

F

P

P

H

H

M

R

T

T

V

I

L

L

E

E

N

N

A

T

A

E

L

Y

A

G

P

L

R

E

I

-

V

V

K

Q

K

N

M

V

S

P

K

K

E

E

E

E

A

R

I

R

E

K

I

R

A

A

K

E

K

K

E

A

L

L

E

D

H

N

V

A

G

N

L

L

G

L

D

D

K

F

F

K

D

D

V

Q

Y

Y

P

P

T

K

K

Q

M

L

S

S

G

G

G

G

Q

M

Q

Q

Q

Q

R

R

L

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

M

N

K

D

P

P

D

E

Y

I

M

L

L

L

L

M

D

D

E

E

V

A

T

F

S

S

A

A

L

L

D

D

P

P

E

L

L

I

V

R

G

R

E

E

V

M

L

Q

D

D

T

E

-

L

L

L

R

E

L

L

L

Q

A

A

E

K

E

F

G

Q

M

K

T

T

M

I

I

I

C

F

V

V

T

S

H

H

E

D

M

L

G

N

F

E

A

A

R

L

D

R

V

I

S

G

D

D

R

R

V

I

A

A

Y

I

F

M

H

K

E

D

G

G

V

K

I

I

A

M

E

Q

I

I

G

G

E

T

A

G

R

E

Q

E

V

I

I

L

T

T

D

N

P

P

Q

A

N

N

P

D

L

Y

T

V

Q

K

K

T

F

F

L

V

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 295, 296, 297, 298, 357, 372

7ahdC Opua (e190q) occluded (see paper)
41% identity, 88% coverage: 20:229/240 of query aligns to 45:256/260 of 7ahdC

query
sites
7ahdC

V

T

S

N

L

F

D

E

V

I

K

N

K

E

G

G

E

E

V

I

V

F

S

V

V

I

I

M

G

G

G

L

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

L

Y

R

C

L

I

L

N

N

A

R

-

L

I

I

E

E

P

P

I

T

N

S

K

-

G

G

K

K

I

I

L

F

V

I

D

D

D

N

V

Q

D

D

V

V

H

A

A

T

K

-

E

-

T

L

N

N

K

K

D

E

K

D

L

L

-

L

-

Q

-

V

-

R

R

R

Q

K

K

T

L

M

G

S

M

M

V

V

F

F

Q
|
Q

Q

N

W

F

N

G

S

L

F

F

P

P

H

H

M

R

T

T

V

I

L

L

E

E

N

N

A

T

A

E

L

Y

A

G

P

L

R

E

I

-

V

V

K

Q

K

N

M

V

S

P

K

K

E

E

E

E

A

R

I

R

E

K

I

R

A

A

K

E

K

K

E

A

L

L

E

D

H

N

V

A

G

N

L

L

G

L

D

D

K

F

F

K

D

D

V

Q

Y

Y

P

P

T
x
K

K
x
Q

M

L

S
|
S

G

G

G
|
G

Q
x
M

Q

Q

Q

Q

R

R

L

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

M

N

K

D

P

P

D

E

Y

I

M

L

L

L

L

M

D

D

E
x
Q

V

A

T

F

S

S

A

A

L

L

D

D

P

P

E

L

L

I

V

R

G

R

E

E

V

M

L

Q

D

D

T

E

-

L

L

L

R

E

L

L

L

Q

A

A

E

K

E

F

G

Q

M

K

T

T

M

I

I

I

C

F

V

V

T

S

H
|
H

E

D

M

L

G

N

F

E

A

A

R

L

D

R

V

I

S

G

D

D

R

R

V

I

A

A

Y

I

F

M

H

K

E

D

G

G

V

K

I

I

A

M

E

Q

I

I

G

G

E

T

A

G

R

E

Q

E

V

I

I

L

T

T

D

N

P

P

Q

A

N

N

binding adenosine-5'-triphosphate: S61 (= S36), G62 (= G37), S63 (= S38), G64 (= G39), K65 (= K40), S66 (= S41), T67 (= T42), Q111 (= Q84), K161 (≠ T135), Q162 (≠ K136), S164 (= S138), G166 (= G140), M167 (≠ Q141), Q188 (≠ E162), H221 (= H194)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 29, 39

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
38% identity, 96% coverage: 1:230/240 of query aligns to 3:236/615 of 5lilA

query
sites
5lilA

M

I

I

I

S

E

V

I

Q

K

N

Q

V

L

H

N

K

R

S

Y

F
|
F

G

G

D

E

L

G

E

E

-

N

-

R

-

V

-

H

V
|
V

L

L

K

K

G

D

V

I

S

S

L

L

D

S

V

I

K

E

K

R

G

G

E

D

V

F

V

V

S

A

V

I

I

M

G

G

G

Q

S
|
S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

L

L

L

M

Y

N

C

I

I

I

N

G

A

C

I

L

E

D

P

T

I

A

N

T

K

G

G

G

K

S

I

S

L

K

V

I

D

D

D

-

V

-

D

-

V

-

H

-

A

G

K

K

E

E

T

T

-

I

-

E

-

L

N

T

K

N

D

D

K

Q

L

L

R

S

-

D

-

L

-

R

-

S

Q

Q

K

K

L

F

G

G

M

F

V

I

F

F

Q
|
Q

Q

R

W

Y

N

N

S

L

F

L

P

S

H

S

M

L

T

T

V

A

L

A

E

E

N

N

A

V

A

A

L

L

A

-

P

P

R

A

I

I

V

Y

K

A

K

G

M

M

S

P

K

Q

E

S

E

Q

A

R

I

L

E

E

I

R

A

A

K

K

K

Q

E

L

L

L

E

E

H

K

V

L

G

G

L

L

G

G

D

D

K
|
K

F

W

D

Q

V
x
N

Y

K

P

P

T

N

K
x
Q

M
x
L

S
|
S

G
|
G

G
|
G

Q
|
Q

Q

Q

Q

Q

R

R

L

V

A

S

I

I

A

A

R

R

A

A

L

L

A

M

M

N

K

G

P

G

D

E

Y

I

M

I

L

L

L

A

D
|
D

E
x
Q

V

P

T

T

S

G

A
|
A

L

L

D

D

P

S

E

H

L

S

V

G

G

E

E

N

V

V

L

M

D

E

T

I

L

L

R

R

L

Q

L

L

A

H

E

E

E

E

G

G

M

H

T

T

M

I

I

I

C

M

V

V

T

T

H
|
H

E

D

M

K

G

H

F

I

A

A

R

A

D

S

V

-

S

A

D

N

R

R

V

I

A

I

Y

E

F

I

H

K

E

D

G

G

V

E

I

I

A

I

E

S

I

D

G

T

E

Q

A

K

R

R

Q

Q

V

V

I

K

T

S

D

A

P

V

Q

K

N

N

P

P

binding adenosine-5'-triphosphate: F13 (= F11), V22 (= V16), S42 (= S36), G43 (= G37), S44 (= S38), G45 (= G39), K46 (= K40), S47 (= S41), T48 (= T42), Q92 (= Q84), K136 (= K129), N139 (≠ V132), Q143 (≠ K136), L144 (≠ M137), S145 (= S138), G146 (= G139), G147 (= G140), Q148 (= Q141), Q169 (≠ E162), A173 (= A166), H201 (= H194)
binding magnesium ion: S47 (= S41), Q92 (= Q84), D168 (= D161)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
38% identity, 96% coverage: 1:230/240 of query aligns to 3:236/592 of 5lj7A

query
sites
5lj7A

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binding adenosine-5'-triphosphate: F13 (= F11), V22 (= V16), S42 (= S36), G43 (= G37), S44 (= S38), G45 (= G39), K46 (= K40), S47 (= S41), T48 (= T42), Q92 (= Q84), K136 (= K129), N142 (≠ T135), Q143 (≠ K136), L144 (≠ M137), S145 (= S138), G146 (= G139), G147 (= G140), Q148 (= Q141), Q169 (≠ E162), A173 (= A166), H201 (= H194)
binding magnesium ion: S47 (= S41), Q92 (= Q84), D168 (= D161)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
40% identity, 90% coverage: 1:217/240 of query aligns to 3:220/223 of 2pclA

query
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2pclA

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binding magnesium ion: E165 (= E162), P166 (≠ V163), T167 (= T164), T196 (= T193)

P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 100% coverage: 1:239/240 of query aligns to 17:251/378 of P69874

query
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C26 (≠ S10) mutation to A: Lower ATPase activity and transport efficiency.
F27 (= F11) mutation to L: Lower ATPase activity and transport efficiency.
F45 (≠ V29) mutation to L: Lower ATPase activity and transport efficiency.
C54 (≠ S38) mutation to T: Loss of ATPase activity and transport.
L60 (= L44) mutation to F: Lower ATPase activity and transport efficiency.
L76 (≠ V60) mutation to P: Lower ATPase activity and transport efficiency.
V135 (= V124) mutation to M: Loss of ATPase activity and transport.
D172 (= D161) mutation to N: Loss of ATPase activity and transport.

Sites not aligning to the query:

276 C→A: Lower ATPase activity and transport efficiency.
297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.

P0AAH0 Phosphate import ATP-binding protein PstB; ABC phosphate transporter; Phosphate-transporting ATPase; EC 7.3.2.1 from Escherichia coli (strain K12) (see paper)
35% identity, 98% coverage: 2:237/240 of query aligns to 11:253/257 of P0AAH0

query
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P0AAH0

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