SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1476 FitnessBrowser__WCS417:GFF1476 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0ABK5 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; S-carboxymethylcysteine synthase; Sulfate starvation-induced protein 5; SSI5; EC 2.5.1.47; EC 4.5.1.5 from Escherichia coli (strain K12) (see 5 papers)
71% identity, 100% coverage: 1:323/324 of query aligns to 1:322/323 of P0ABK5

query
sites
P0ABK5

M
|
M

S

S

R

K

I

I

F

F

A

E

D

D

N

N

A

S

H

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

Q

R

I

L

N

N

R

R

I

I

A

G

P

-

R

-

G

N

V

G

T

R

I

I

L

L

A

A

K

K

I

V

E

E

G

S

R

R

N

N

P

P

G

S

Y

F

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

S

K

T

R

G

G

K

V

L

L

K

K

P

P

G

G

M

V

T

E

I

L

V

V

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

V

L

L

L

K

K

A

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

Q

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

N

P

P

A

E

T

K

Y

Y

F

L

M

L

P

L

A

Q

Q

Q

F

F

E

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

A

Q

V

V

D

D

V

V

L

F

V

I

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

S

S

R

R

Y

Y

I

I

K

K

N

G

I

T

A

K

G

G

K

K

P

T

-

D

I

L

L

I

S

S

V

V

A

A

V

V

E

E

P

P

I

T

V

D

S

S

P

P

V

V

I

I

T

A

Q

Q

A

A

L

L

A

A

G

G

E

E

I

I

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

A

N

N

L

L

D

D

L

L

S

K

M

L

V

V

D

D

R

K

V

V

E

I

L

G

V

I

T

T

D

N

D

E

E

E

S

A

K

I

A

S

M

T

A

A

L

R

R

R

L

L

M

M

Q

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S

S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

L

R

K

L

L

A

Q

E

E

K

D

P

E

E

S

M

F

Q

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P

P

D

S

S

S

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

S

A

D

D

L

L

F

F

T

T

E

E

Q

K

E

E

N

L

Q

Q

M1 (= M1) modified: Initiator methionine, Removed
K42 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: Still stimulates tRNase activity of CdiA-CT in vitro and in vivo.

P0A1E3 Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; EC 2.5.1.47 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 4 papers)
69% identity, 100% coverage: 1:323/324 of query aligns to 1:322/323 of P0A1E3

query
sites
P0A1E3

M
|
M

S

S

R

K

I

I

F

Y

A

E

D

D

N

N

A

S

H

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

Q

R

I

L

N

N

R

R

I

I

A

G

P

-

R

-

G

N

V

G

T

R

I

I

L

L

A

A

K

K

I

V

E

E

G

S

R

R

N

N

P

P

G

S

Y

F

S

S

V

V

K

K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

S

K

T

R

G

G

K

V

L

L

K

K

P

P

G

G

M

V

T

E

I

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

V

L

L

L

K

K

A

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

Q

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

Q

T

K

Y

Y

F

L

M

L

P

L

A

Q

Q

Q

F

F

E

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

A

Q

V

V

D

D

V

V

L

F

V

I

A

S

G

G

V

V

G

G

T

T

G

G

T

T

I

L

T

T

G

G

V

V

S

T

R

R

Y

Y

I

I

K

K

N

G

I

T

A

K

G

G

K

K

-

T

P

D

I

L

L

I

S

T

V

V

A

A

V

V

E

E

P

P

I

T

V

D

S

S

P

P

V

V

I

I

T

A

Q

Q

A

A

L

L

A

A

G

G

E

E

I

I

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

N

N

L

L

D

D

L

L

S

K

M

L

V

I

D

D

R

K

V

V

E

V

L

G

V

I

T

T

D

N

D

E

E

E

S

A

K

I

A

S

M

T

A

A

L

R

R

R

L

L

M

M

Q

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

L

R

K

L

L

A

Q

E

E

K

D

P

E

E

S

M

F

Q

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P

P

D

S

S

S

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

S

A

D

D

L

L

F

F

T

T

E

E

Q

K

E

E

N

L

Q

Q

M1 (= M1) modified: Initiator methionine, Removed
N72 (= N74) binding
S273 (= S274) binding

6z4nAAA structure of oass complexed with upar inhibitor (see paper)
70% identity, 99% coverage: 1:321/324 of query aligns to 2:321/321 of 6z4nAAA

query
sites
6z4nAAA

M

M

S

S

R

K

I

I

F

Y

A

E

D

D

N

N

A

S

H

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

Q

R

I

L

N

N

R

R

I

I

A

G

P

-

R

-

G

N

V

G

T

R

I

I

L

L

A

A

K

K

I

V

E

E

G

S

R

R

N

N

P

P

G

S

Y

F

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

S

K

T

R

G

G

K

V

L

L

K

K

P

P

G

G

M

V

T

E

I

L

V

V

E

E

P

P

T
|
T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

V

L

L

L

K

K

A

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

Q

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

Q

T

K

Y

Y

F

L

M

L

P

L

A

Q

Q
|
Q

F
|
F

E

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

A

Q

V

V

D

D

V

V

L

F

V

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

T

R

R

Y

Y

I

I

K

K

N

G

I

T

A

K

G

G

K

K

P

T

-

D

I

L

L

I

S

T

V

V

A

A

V

V

E

E

P

P

I

T

V

D

S

S

P

P

V

V

I

I

T

A

Q

Q

A

A

L

L

A

A

G

G

E

E

I

I

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

G

N

N

L

L

D

D

L

L

S

K

M

L

V

I

D

D

R

K

V

V

E

V

L

G

V

I

T

T

D

N

D

E

E

E

S

A

K

I

A

S

M

T

A

A

L

R

R

R

L

L

M

M

Q

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

L

R

K

L

L

A

Q

E

E

K

D

P

E

E

S

M

F

Q

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P
|
P

D

S

S

S

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

S

A

D

D

L

L

F

F

T

T

E

E

Q

K

E

E

binding pyridoxal-5'-phosphate: K43 (= K44), N73 (= N74), V177 (= V178), G178 (= G179), T179 (= T180), G180 (= G181), T182 (= T183), G230 (= G230), S274 (= S274), P301 (= P301)
binding (1~{S},2~{S})-1-[(4-methylphenyl)methyl]-2-phenyl-cyclopropane-1-carboxylic acid: K43 (= K44), T70 (= T71), G72 (= G73), N73 (= N74), T74 (= T75), Q144 (= Q145), F145 (= F146), Q229 (= Q229), G230 (= G230), I231 (= I231), A233 (= A233)

1d6sA Crystal structure of the k41a mutant of o-acetylserine sulfhydrylase complexed in external aldimine linkage with methionine (see paper)
69% identity, 99% coverage: 2:323/324 of query aligns to 1:321/322 of 1d6sA

query
sites
1d6sA

S

S

R

K

I

I

F

Y

A

E

D

D

N

N

A

S

H

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

Q

R

I

L

N

N

R

R

I

I

A

G

P

-

R

-

G

N

V

G

T

R

I

I

L

L

A

A

K

K

I

V

E

E

G

S

R

R

N

N

P

P

G

S

Y

F

S

S

V

V

K
x
A

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

S

K

T

R

G

G

K

V

L

L

K

K

P

P

G

G

M

V

T

E

I

L

V

V

E

E

P

P

T
|
T

S
x
N

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

V

L

L

L

K

K

A

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

Q

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

Q

T

K

Y

Y

F

L

M

L

P

L

A

Q

Q
|
Q

F
|
F

E

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

A

Q

V

V

D

D

V

V

L

F

V

I

A

S

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

T

R

R

Y

Y

I

I

K

K

N

G

I

T

A

K

G

G

K

K

-

T

P

D

I

L

L

I

S

T

V

V

A

A

V

V

E

E

P

P

I

T

V

D

S

S

P

P

V

V

I

I

T

A

Q

Q

A

A

L

L

A

A

G

G

E

E

I

I

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

N

N

L

L

D

D

L

L

S

K

M

L

V

I

D

D

R

K

V

V

E

V

L

G

V

I

T

T

D

N

D

E

E

E

S

A

K

I

A

S

M

T

A

A

L

R

R

R

L

L

M

M

Q

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

L

R

K

L

L

A

Q

E

E

K

D

P

E

E

S

M

F

Q

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P
|
P

D

S

S

S

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

S

A

D

D

L

L

F

F

T

T

E

E

Q

K

E

E

N

L

Q

Q

active site: A41 (≠ K44), G228 (= G230)
binding methionine: T68 (= T71), N69 (≠ S72), N71 (= N74), T72 (= T75), Q142 (= Q145), F143 (= F146), G176 (= G179), G228 (= G230)
binding pyridoxal-5'-phosphate: N71 (= N74), G176 (= G179), T177 (= T180), G178 (= G181), T180 (= T183), G228 (= G230), S272 (= S274), P299 (= P301)

1fcjA Crystal structure of oass complexed with chloride and sulfate (see paper)
69% identity, 94% coverage: 2:304/324 of query aligns to 1:302/302 of 1fcjA

query
sites
1fcjA

S

S

R

K

I

I

F

Y

A

E

D

D

N

N

A

S

H

L

S

T

I

I

G

G

N

H

T

T

P

P

L

L

V

V

Q

R

I

L

N

N

R

R

I

I

A

G

P

-

R

-

G

N

V

G

T

R

I

I

L

L

A

A

K

K

I

V

E

E

G

S

R

R

N

N

P

P

G

S

Y

F

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

I

W

W

D

D

A

A

E

E

S

K

T

R

G

G

K

V

L

L

K

K

P

P

G

G

M

V

T

E

I

L

V

V

E

E

P

P

T

T

S

N

G
|
G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

L

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

I

E

E

R

R

K

K

V

L

L

L

K

K

A

A

L

L

G

G

A

A

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

Q

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

Q

T

K

Y

Y

F

L

M

L

P

L

A

Q

Q
|
Q

F

F

E

S

N

N

P

P

A

A

N

N

P

P

A

E

I

I

H

H

E

E

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

D

G

G

A

Q

V

V

D

D

V

V

L

F

V

I

A

S

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

L

T

T

G

G

V

V

S

T

R

R

Y

Y

I

I

K

K

N

G

I

T

A

K

G

G

K

K

-

T

P

D

I

L

L

I

S

T

V

V

A

A

V

V

E

E

P

P

I

T

V

D

S

S

P

P

V

V

I

I

T

A

Q

Q

A

A

L

L

A

A

G

G

E

E

I

I

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

N

N

L

L

D

D

L

L

S

K

M

L

V

I

D

D

R

K

V

V

E

V

L

G

V

I

T

T

D

N

D

E

E

E

S

A

K

I

A

S

M

T

A

A

L

R

R

R

L

L

M

M

Q

E

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

L

R

K

L

L

A

Q

E

E

K

D

P

E

E

S

M

F

Q

T

G

N

K

K

T

N

I

I

V

V

I

I

L

L

P
|
P

D

S

S

S

G

G

active site: K41 (= K44), G228 (= G230), S272 (= S274)
binding pyridoxal-5'-phosphate: K41 (= K44), N71 (= N74), V175 (= V178), G176 (= G179), T177 (= T180), G178 (= G181), T180 (= T183), G228 (= G230), S272 (= S274), P299 (= P301)
binding sulfate ion: G70 (= G73), T72 (= T75), Q142 (= Q145)

1y7lA O-acetylserine sulfhydrylase complex (see paper)
68% identity, 96% coverage: 4:313/324 of query aligns to 2:310/310 of 1y7lA

query
sites
1y7lA

I

I

F

Y

A

A

D

D

N

N

A

S

H

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

R

I

L

N

K

R

H

I

F

A

G

P

H

R

N

G

G

V

-

T

N

I

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

G

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

S

K

T

D

G

G

K

T

L

L

K

T

P

K

G

G

M

K

T

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

I

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

K

C

A

G

L

L

G

G

A

V

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K

K

G

G

M
|
M

K

K

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

S

T

R

Y

Y

F

V

M

M

P

L

A

K

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

A

K

V

V

D

D

V

V

L

V

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

N

L

I

D

A

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

I

V

V

E

S

S

P

P

V

V

I

I

T

S

Q

Q

A

T

L

L

A

A

G

G

E

E

I

V

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

K

N

N

L

L

D

D

L

L

S

S

M

I

V

I

D

D

R

R

V

V

E

E

L

T

V

V

T

D

D

S

D

D

E

T

S

A

K

L

A

A

M

T

A

A

L

R

R

R

L

L

M

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

D

R

R

L

L

A

A

E

K

K

L

P

P

E

E

M

F

Q

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

active site: K41 (= K44), S271 (= S274), P298 (= P301)
binding : K41 (= K44), T68 (= T71), S69 (= S72), T72 (= T75), M119 (= M122), Q142 (= Q145)

5dbhX Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with reaction intermediate alpha-aminoacrylate
68% identity, 96% coverage: 4:313/324 of query aligns to 2:310/312 of 5dbhX

query
sites
5dbhX

I

I

F

Y

A

A

D

D

N

N

A

S

H

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

R

I

L

N

K

R

H

I

F

A

G

P

H

R

N

G

G

V

-

T

N

I

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

G

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

S

K

T

D

G

G

K

T

L

L

K

T

P

K

G

G

M

K

T

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

I

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

K

C

A

G

L

L

G

G

A

V

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

S

T

R

Y

Y

F

V

M

M

P

L

A

K

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

A

K

V

V

D

D

V

V

L

V

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
x
S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

N

L

I

D

A

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

I

V

V

E

S

S

P

P

V

V

I

I

T

S

Q

Q

A

T

L

L

A

A

G

G

E

E

I

V

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

K

N

N

L

L

D

D

L

L

S

S

M

I

V

I

D

D

R

R

V

V

E

E

L

T

V

V

T

D

D

S

D

D

E

T

S

A

K

L

A

A

M

T

A

A

L

R

R

R

L

L

M

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

D

R

R

L

L

A

A

E

K

K

L

P

P

E

E

M

F

Q

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: K41 (= K44), T68 (= T71), S69 (= S72), N71 (= N74), T72 (= T75), Q142 (= Q145), G176 (= G179), T177 (= T180), G178 (= G181), S180 (≠ T183), G227 (= G230), S271 (= S274), P298 (= P301)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
67% identity, 97% coverage: 4:316/324 of query aligns to 4:309/310 of 5xoqA

query
sites
5xoqA

I

I

F

F

A

K

D

D

N

N

A

S

H

E

S

S

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

Q

I

I

N

N

R

R

I

L

-

T

A

A

P

G

R

L

G

S

V

S

T

R

I

V

L

L

A

A

K

K

I

I

E

E

G

G

R

R

N

N

P

P

G

A

Y

Y

S

S

V

V

K

K

C

C

R

R

I

I

G

G

A

A

N

A

M

M

I

I

W

W

D

D

A

A

E

E

S

Q

T

S

G

G

K

K

L

L

K

K

P

P

G

G

M

M

T

H

I

V

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

F

V

V

A

C

A

A

R

R

G

G

Y

Y

K

K

L

L

L

T

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

I

E

E

R

R

K

M

V

M

L

L

K

K

A

S

L

F

G

G

A

A

E

D

L

L

V

V

L

L

T

T

E

P

P

G

A

A

K

D

G

G

M
|
M

K

K

G

G

A

A

I

I

E

S

K

K

A

A

G

E

E

E

I

L

V

-

A

A

S

A

D

Q

P

P

A

G

T

-

Y

W

F

F

M

I

P

P

A

Q

Q
|
Q

F

F

E

K

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

V

K

K

T

T

G

G

P

P

E

E

I

I

W

W

N

N

D

D

T

T

D

E

G

G

A

Q

V

V

D

D

V

V

L

F

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

A

R

R

Y

F

I

L

K

K

N

H

I

E

A

K

G

K

K

H

P

P

I

V

L

H

S

V

V

V

A

A

V

V

E

E

P

P

I

A

V

A

S

S

P

P

V

V

I

L

T

A

Q

G

A

G

L

P

A

A

G

G

E
x
R

E

-

I

-

K

-

P

-

S

-

P

-

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

K

D

N

T

L

F

D

D

L

R

S

S

M

V

V

V

D

D

R

E

V

I

E

L

L

S

V

V

T

T

D

D

E

E

S

A

K

I

A

E

M

T

A

A

L

R

R

K

L

L

M

A

Q

M

E

E

G

G

I

I

L

S

C

C

G

G

I

I

S

S

C

C

G

G

A

A

M

M

A

A

V

G

A

A

V

L

R

K

L

V

A

A

E

A

K

R

P

P

E

E

M

F

Q

A

G

G

K

K

T

T

I

I

V

V

V

T

I

V

L

L

P

P

D

D

S

A

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

S

E

D

N

L

L

binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), M123 (= M122), Q144 (= Q145), R218 (≠ E219), H219 (= H226), Q222 (= Q229), G223 (= G230), A226 (= A233)

4zu6X Crystal structure of o-acetylserine sulfhydrylase from haemophilus influenzae in complex with pre-reactive o-acetyl serine, alpha- aminoacrylate reaction intermediate and peptide inhibitor at the resolution of 2.25a
68% identity, 96% coverage: 4:313/324 of query aligns to 3:309/309 of 4zu6X

query
sites
4zu6X

I

I

F

Y

A

A

D

D

N

N

A

S

H

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

R

I

L

N

K

R

H

I

F

A

G

P

H

R

N

G

G

V

-

T

N

I

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

G

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

S

K

T

D

G

G

K

T

L

L

K

T

P

K

G

G

M

K

T

E

I

I

V

V

E

D

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

I

L

T

L

L

T

T

M

M

P

P

A

E

S
x
T

M
|
M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

K

C

A

G

L

L

G

G

A

V

E

N

L

L

V

V

L

L

T

T

E

E

P

G

A

A

K
|
K

G
|
G

M

-

K

-

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

S

T

R

Y

Y

F

V

M

M

P

L

A

K

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

A

K

V

V

D

D

V

V

L

V

V

V

A

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

T
x
S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

N

L

I

D

A

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

I

V

V

E

S

S

P

P

V

V

I

I

T

S

Q

Q

A

T

L

L

A

A

G

G

E

E

I

V

K

K

P

P

S

G

P
|
P

H
|
H

K
|
K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

K

N

N

L

L

D

D

L

L

S

S

M

I

V

I

D

D

R

R

V

V

E

E

L

T

V

V

T

D

D

S

D

D

E

T

S

A

K

L

A

A

M

T

A

A

L

R

R

R

L

L

M

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S
|
S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

D

R

R

L

L

A

A

E

K

K

L

P

P

E

E

M

F

Q

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P
|
P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

binding o-acetylserine: T69 (= T71), S70 (= S72), T73 (= T75), Q141 (= Q145), G175 (= G179), G226 (= G230)
binding pyridoxal-5'-phosphate: K42 (= K44), N72 (= N74), V174 (= V178), G175 (= G179), T176 (= T180), G177 (= G181), G178 (= G182), S179 (≠ T183), G226 (= G230), S270 (= S274), P297 (= P301)
binding : S70 (= S72), T95 (≠ S97), M96 (= M98), K118 (= K120), G119 (= G121), P221 (= P225), H222 (= H226), K223 (= K227), A229 (= A233)

4oreX Cyrstal structure of o-acetylserine sulfhydrylase ternary complex from haemophilus influenzae at 2.2 a
68% identity, 96% coverage: 4:313/324 of query aligns to 2:308/308 of 4oreX

query
sites
4oreX

I

I

F

Y

A

A

D

D

N

N

A

S

H

Y

S

S

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

R

I

L

N

K

R

H

I

F

A

G

P

H

R

N

G

G

V

-

T

N

I

V

L

V

A

V

K

K

I

I

E

E

G

G

R

R

N

N

P

P

G

S

Y

Y

S

S

V

V

K
|
K

C

C

R

R

I

I

G

G

A

A

N

N

M

M

I

V

W

W

D

Q

A

A

E

E

S

K

T

D

G

G

K

T

L

L

K

T

P

K

G

G

M

K

T

E

I

I

V

V

E

D

P

A

T
|
T

S

-

G
|
G

N

N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

Y

V

V

A

A

R

R

G

G

Y

Y

K

K

L

I

L

T

L

L

T

T

M

M

P
|
P

A

E

S

T

M

M

S

S

I

L

E

E

R

R

R

K

K

R

V

L

L

L

K

C

A

G

L

L

G

G

A

V

E

N

L

L

V

V

L

L

T

T

E

E

P

-

A

A

K
|
K

G
|
G

M
|
M

K

K

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

I

V

V

A

A

S

S

D

D

P

P

A

S

T

R

Y

Y

F

V

M

M

P

L

A

K

Q

Q

F
|
F

E

E

N

N

P

P

A

A

N

N

P

P

A

Q

I

I

H

H

E

R

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

D

T

T

D

D

G

G

A

K

V

V

D

D

V

V

L

V

V

V

A

A

G

G

V

V

G

G

T

T

G

G

T

S

I

I

T

T

G

G

V

I

S

S

R

R

Y

A

I

I

K

K

N

L

I

D

A

F

G

G

K

K

P

Q

I

I

L

T

S

S

V

V

A

A

V

V

E

E

P

P

I

V

V

E

S

S

P

P

V

V

I

I

T

S

Q

Q

A

T

L

L

A

A

G

G

E

E

I

V

K

K

P

P

S
x
G

P
|
P

H
|
H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

I

P

P

K

K

N

N

L

L

D

D

L

L

S

S

M

I

V

I

D

D

R

R

V

V

E

E

L

T

V

V

T

D

D

S

D

D

E

T

S

A

K

L

A

A

M

T

A

A

L

R

R

R

L

L

M

M

Q

A

E

E

G

G

I

I

L

L

C

A

G

G

I

I

S

S

C

S

G

G

A

A

M

V

A

A

V

A

A

A

V

D

R

R

L

L

A

A

E

K

K

L

P

P

E

E

M

F

Q

A

G

D

K

K

T

L

I

I

V

V

I

I

L

L

P

P

D

S

S

A

G

S

E

E

R

R

Y

Y

L

L

S

S

S

T

M

A

L

L

F

F

binding o-acetylserine: K115 (= K120), G116 (= G121), M117 (= M122)
binding : K41 (= K44), T68 (= T71), G69 (= G73), T71 (= T75), P91 (= P95), M117 (= M122), F141 (= F146), G219 (≠ S224), P220 (= P225), H221 (= H226), G225 (= G230), A228 (= A233)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
56% identity, 98% coverage: 6:322/324 of query aligns to 76:387/392 of P47999

query
sites
P47999

A

A

D

D

N

N

A

A

-

A

H

Q

S

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

Q

Y

I

L

N

N

R

N

I

V

A

V

P

K

R

G

G

C

V

V

-

A

T

S

I

V

L

A

A

A

K

K

I

L

E

E

G

I

R

M

N

E

P

P

G

C

Y

C

S

S

V

V

K

K

C

D

R

R

I

I

G

G

A

Y

N

S

M

M

I

I

W

T

D

D

A

A

E

E

S

E

T

K

G

G

K

L

L

I

K

T

P

P

G

G

M

K

T

S

I

V

-

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

F

F

V

I

A

A

A

S

R

K

G

G

Y

Y

K

K

L

L

L

I

L

L

T

T

M

M

P

P

A

A

S

S

M

M

S

S

I

L

E

E

R

R

K

V

V

L

L

L

K

R

A

A

L

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

E

E

P

P

A

A

K

K

G

G

M

M

K

T

G

G

A

A

I

I

E

Q

K

K

A

A

G

E

E

E

I

I

V

L

A

K

S

K

D

T

P

P

A

N

T

S

Y

Y

F

-

M

M

P

L

A

Q

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

E

D

D

T

T

D

R

G

G

A

K

V

I

D

D

V

I

L

L

V

V

A

A

G

G

V

I

G

G

T

T

G

G

T

T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

I

I

K

K

N

E

I

-

A

-

G

R

K

K

P

P

I

E

L

L

S

K

V

V

-

I

A

G

V

V

E

E

P

P

I

T

V

E

S

S

P

A

V

I

I

L

T

S

Q

G

A

G

L

-

A

-

G

-

E

-

I

-

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

K

K

N

N

L

L

D

D

L

L

S

A

M

I

V

V

D

D

R

E

V

Y

E

I

L

A

V

I

T

S

D

S

D

E

E

E

S

A

K

I

A

E

M

T

A

S

L

K

R

Q

L

L

M

A

Q

L

E

Q

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S

S

C

S

G

G

A

A

M

A

A

A

V

A

A

A

V

I

R

Q

L

V

A

A

E

K

K

R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

I

V

A

V

V

I

V

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

Q

L

L

F

F

S

Q

D

S

L

I

F

R

T

E

E

E

Q

C

E

E

N

Q

Sites not aligning to the query:

61 modified: N-acetylalanine

4aecA Crystal structure of the arabidopsis thaliana o-acetyl-serine-(thiol)- lyasE C (see paper)
55% identity, 97% coverage: 6:319/324 of query aligns to 14:322/323 of 4aecA

query
sites
4aecA

A

A

D

D

N

N

-

V

A

S

H

Q

S

L

I

I

G

G

N

K

T

T

P

P

L

M

V

V

Q

Y

I

L

N

N

R

S

I

I

A

A

P

K

R

G

G

C

V

V

-

A

T

N

I

I

L

A

A

A

K

K

I

L

E

E

G

I

R

M

N

E

P

P

G

C

Y

C

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

Y

N

S

M

M

I

V

W

T

D

D

A

A

E

E

S

Q

T

K

G

G

K

F

L

I

K

S

P

P

G

G

M

K

T

S

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

F

F

V

I

A

A

A

S

R

R

G

G

Y

Y

K

R

L

L

L

I

L

L

T

T

M

M

P

P

A

A

S

S

M

M

S

S

I

M

E

E

R

R

K

V

V

L

L

L

K

K

A

A

L

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

E

D

P

P

A

A

K

K

G

G

M

M

K

T

G

G

A

A

I

V

E

Q

K

K

A

A

G

E

E

E

I

I

V

L

A

K

S

N

D

T

P

P

A

D

T

A

Y

Y

F

M

M

L

P

-

A

Q

Q

Q

F

F

E

D

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

D

D

D

T

T

D

K

G

G

A

K

V

V

D

D

V

I

L

F

V

V

A

A

G

G

V
x
I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

G

R

R

Y

F

I

I

K

K

N

E

I

K

A

N

G

P

K

K

P

T

I

Q

L

V

S

-

V

I

A

G

V

V

E

E

P

P

I

T

V

E

S

S

P

D

V

I

I

L

T

S

Q

G

A

G

L

-

A

-

G

-

E

-

I

-

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

K

N

N

L

L

D

D

L

Q

S

K

M

I

V

M

D

D

R

E

V

V

E

I

L

A

V

I

T

S

D

S

D

E

E

E

S

A

K

I

A

E

M

T

A

A

L

K

R

Q

L

L

M

A

Q

L

E

K

E

E

G

G

I

L

L

M

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

A

A

A

V

A

A

A

V

I

R

K

L

V

A

A

E

K

K

R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

I

V

A

V

V

I

V

L

F

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

P

L

L

F

F

S

Q

D

S

L

I

F

R

T

E

E

E

active site: K54 (= K44), S277 (= S274)
binding pyridoxal-5'-phosphate: K54 (= K44), N85 (= N74), I188 (≠ V178), G189 (= G179), T190 (= T180), G191 (= G181), G192 (= G182), T193 (= T183), G233 (= G230), S277 (= S274), P304 (= P301)

2isqA Crystal structure of o-acetylserine sulfhydrylase from arabidopsis thaliana in complex with c-terminal peptide from arabidopsis serine acetyltransferase (see paper)
55% identity, 99% coverage: 2:321/324 of query aligns to 1:314/320 of 2isqA

query
sites
2isqA

S

S

R

R

I

I

F

A

A

K

D

D

N

V

A

T

H

E

S

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

I

L

N

N

R

N

I

V

A

A

P

E

R

G

G

C

V

V

-

G

T

R

I

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

G

C

Y

S

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

F

N

S

M

M

I

I

W

S

D

D

A

A

E

E

S

K

T

K

G

G

K

L

L

I

K

K

P

P

G

G

M

E

T

S

I

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

F

F

V

T

A

A

R

K

G

G

Y

Y

K

K

L

L

L

I

T

T

M

M

P

P

A

A

S

S

M

M

S

S

I

T

E

E

R

R

K

I

V

I

L

L

K

L

A

A

L

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

E

D

P

P

A

A

K

K

G
|
G

M
|
M

K
|
K

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

I

V

L

A

A

S

K

D

T

P

P

A

N

T

G

Y

Y

F

-

M

M

P

L

A

Q

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

A

K

V

I

D

D

V

G

L

F

V

V

A

S

G
|
G

V

I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

N

E

I

Q

A

N

G

A

K

N

P

V

I

K

L

L

S

-

V

Y

A

G

V

V

E

E

P

P

I

V

V

E

S

S

P

A

V

I

I

L

T

S

Q

G

A

G

L

-

A

-

G

-

E

-

I

-

K

K

P

P

S
x
G

P
|
P

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

N

V

L

L

D

N

L

V

S

D

M

L

V

I

D

D

R

E

V

V

E

V

L

Q

V

V

T

S

D

S

D

D

E

E

S

S

K

I

A

D

M

M

A

A

L

R

R

Q

L

L

M

A

Q

L

E

K

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

A

A

A

V

A

A

A

V

I

R

K

L

L

A

A

E

Q

K

R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

F

V

V

V

A

I

I

L

F

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

V

L

L

F

F

S

D

D

A

L

T

F

R

T

K

E

E

Q

A

E

E

active site: K44 (= K44), S267 (= S274)
binding pyridoxal-5'-phosphate: K44 (= K44), N75 (= N74), G177 (= G177), G179 (= G179), T180 (= T180), G181 (= G181), T183 (= T183), G223 (= G230), S267 (= S274), P294 (= P301)
binding : T72 (= T71), S73 (= S72), G74 (= G73), T76 (= T75), G122 (= G121), M123 (= M122), K124 (= K123), G217 (≠ S224), P218 (= P225), H219 (= H226), Q222 (= Q229), G223 (= G230)

P47998 Cysteine synthase 1; At.OAS.5-8; Beta-substituted Ala synthase 1;1; ARAth-Bsas1;1; CSase A; AtCS-A; Cys-3A; O-acetylserine (thiol)-lyase 1; OAS-TL A; O-acetylserine sulfhydrylase; Protein ONSET OF LEAF DEATH 3; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see 3 papers)
55% identity, 99% coverage: 2:321/324 of query aligns to 3:316/322 of P47998

query
sites
P47998

S

S

R

R

I

I

F

A

A

K

D

D

N

V

A

T

H

E

S

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

I

L

N

N

R

N

I

V

A

A

P

E

R

G

G

C

V

V

-

G

T

R

I

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

G

C

Y

S

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

F

N

S

M

M

I

I

W

S

D

D

A

A

E

E

S

K

T

K

G

G

K

L

L

I

K

K

P

P

G

G

M

E

T

S

I

V

-

L

V

I

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

F

F

V

T

A

A

R

K

G

G

Y

Y

K

K

L

L

L

I

T

T

M

M

P

P

A

A

S

S

M

M

S

S

I

T

E

E

R

R

K

I

V

I

L

L

K

L

A

A

L

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

E

D

P

P

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

I

V

L

A

A

S

K

D

T

P

P

A

N

T

G

Y

Y

F

-

M

M

P

L

A

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H
|
H

E

Y

K

E

T

T

G
|
G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

A

K

V

I

D

D

V

G

L

F

V

V

A

S

G

G

V

I

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

N

E

I

Q

A

N

G

A

K

N

P

V

I

K

L

L

S

-

V

Y

A

G

V

V

E

E

P

P

I

V

V

E

S

S

P

A

V

I

I

L

T

S

Q

G

A

G

L

-

A

-

G

-

E

-

I

-

K
|
K

P

P

S

G

P

P

H
|
H

K
|
K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

N

V

L

L

D

N

L

V

S

D

M

L

V

I

D

D

R

E

V

V

E

V

L

Q

V

V

T

S

D

S

D

D

E

E

S

S

K

I

A

D

M

M

A

A

L

R

R

Q

L

L

M

A

Q

L

E

K

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

A

A

A

V

A

A

A

V

I

R

K

L

L

A

A

E

Q

K

R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

F

V

V

V

A

I

I

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

V

L

L

F

F

S

D

D

A

L

T

F

R

T

K

E

E

Q

A

E

E

K46 (= K44) modified: N6-(pyridoxal phosphate)lysine; mutation to A: No cysteine synthase activity.
T74 (= T71) mutation to A: Strong reduction of cysteine synthase activity.; mutation to S: Reduction of cysteine synthase activity.
S75 (= S72) mutation S->A,N,T: Strong reduction of cysteine synthase activity.
N77 (= N74) binding ; mutation to A: Reduction of cysteine synthase activity.; mutation to D: Strong reduction of cysteine synthase activity.
T78 (= T75) mutation T->A,S: Reduction of cysteine synthase activity.
Q147 (= Q145) mutation Q->A,E: Strong reduction of cysteine synthase activity.
H157 (= H155) mutation H->Q,N: Slight reduction of cysteine synthase activity.
G162 (= G160) mutation to E: In old3-1; displays a early leaf death phenotype. Abolishes cysteine synthase activity.
GTGGT 181:185 (= GTGGT 179:183) binding
T182 (= T180) mutation T->A,S: Slight reduction of cysteine synthase activity.
T185 (= T183) mutation T->A,S: Strong reduction of cysteine synthase activity.
K217 (= K222) mutation to A: Impaired interaction with SAT1.
H221 (= H226) mutation to A: Impaired interaction with SAT1.
K222 (= K227) mutation to A: Impaired interaction with SAT1.
S269 (= S274) binding ; mutation to A: Strong reduction of cysteine synthase activity.; mutation to T: Reduction of cysteine synthase activity.

1z7yA Crystal structure of the arabidopsis thaliana o-acetylserine sulfhydrylase k46a mutant (see paper)
55% identity, 99% coverage: 2:321/324 of query aligns to 1:314/320 of 1z7yA

query
sites
1z7yA

S

S

R

R

I

I

F

A

A

K

D

D

N

V

A

T

H

E

S

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

I

L

N

N

R

N

I

V

A

A

P

E

R

G

G

C

V

V

-

G

T

R

I

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

G

C

Y

S

S

S

V

V

K
x
A

C

D

R

R

I

I

G

G

A

F

N

S

M

M

I

I

W

S

D

D

A

A

E

E

S

K

T

K

G

G

K

L

L

I

K

K

P

P

G

G

M

E

T

S

I

V

-

L

V

I

E

E

P

P

T

T

S

S

G
|
G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

F

F

V

T

A

A

R

K

G

G

Y

Y

K

K

L

L

L

I

T

T

M

M

P

P

A

A

S

S

M

M

S

S

I

T

E

E

R

R

K

I

V

I

L

L

K

L

A

A

L

F

G

G

A

V

E

E

L

L

V

V

L

L

T

T

E

D

P

P

A

A

K

K

G

G

M

M

K

K

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

I

V

L

A

A

S

K

D

T

P

P

A

N

T

G

Y

Y

F

-

M

M

P

L

A

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

K

D

G

T

T

D

G

G

G

A

K

V

I

D

D

V

G

L

F

V

V

A

S

G

G

V
x
I

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

N

E

I

Q

A

N

G

A

K

N

P

V

I

K

L

L

S

-

V

Y

A

G

V

V

E

E

P

P

I

V

V

E

S

S

P

A

V

I

I

L

T

S

Q

G

A

G

L

-

A

-

G

-

E

-

I

-

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

S

N

V

L

L

D

N

L

V

S

D

M

L

V

I

D

D

R

E

V

V

E

V

L

Q

V

V

T

S

D

S

D

D

E

E

S

S

K

I

A

D

M

M

A

A

L

R

R

Q

L

L

M

A

Q

L

E

K

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

A

A

A

V

A

A

A

V

I

R

K

L

L

A

A

E

Q

K

R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

F

V

V

V

A

I

I

L

F

P
|
P

D
x
S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

V

L

L

F

F

S

D

D

A

L

T

F

R

T

K

E

E

Q

A

E

E

active site: A44 (≠ K44), S267 (= S274)
binding n-[(3-hydroxy-2-methyl-5-{[(trihydroxyphosphoranyl)oxy]methyl}pyridin-4-yl)methylene]methionine: G74 (= G73), N75 (= N74), T76 (= T75), Q145 (= Q145), I178 (≠ V178), G179 (= G179), T180 (= T180), G181 (= G181), T183 (= T183), G223 (= G230), S267 (= S274), P294 (= P301), S295 (≠ D302)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
55% identity, 97% coverage: 4:316/324 of query aligns to 3:308/310 of P9WP55

query
sites
P9WP55

I

I

F

A

A

E

D

D

N

I

A

T

H

Q

S

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

R

I

L

N

R

R

R

I

V

A

T

P

D

R

G

G

A

V

V

T

A

-

D

I

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

G

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

N

A

M

M

I

L

W

Q

D

A

A

A

E

E

S

Q

T

A

G

G

K

L

L

I

K

K

P

P

G

D

M

T

T

I

I

I

V

L

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

Y

Y

K

R

L

C

L

V

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

V

L

L

L

K

R

A

A

L

Y

G

G

A

A

E

E

L

L

V

I

L

L

T

T

E

P

P

G

A

A

K

D

G

G

M

M

K

S

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

L

V

A

A

K

S

T

D

D

P

-

A

Q

T

R

Y

Y

F

F

M

V

P

P

A

Q

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

N

R

D

D

T

T

D

D

G

G

A

K

V

V

D

D

V

I

L

V

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

N

E

I

-

A

-

G

R

K

K

P

P

I

S

L

A

S

R

-

F

V

V

A

A

V

V

E

E

P

P

I

A

V

A

S

S

P

P

V

V

I

L

T

S

Q

G

A

G

L

Q

A

K

G

G

E

-

I

-

K

-

P

-

S

-

P

P

H

H

K

P

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

K

P

N

V

L

L

D

D

L

Q

S

D

M

L

V

V

D

D

R

E

V

I

E

I

L

T

V

V

T

G

D

N

D

E

E

D

S

A

K

L

A

N

M

V

A

A

L

R

R

R

L

L

M

A

Q

R

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

T

A

V

V

A

A

A

V

L

R

Q

L

V

A

A

E

R

K

R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P

P

D

D

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

P

L

L

F

F

S

A

D

D

L

V

K44 (= K44) modified: N6-(pyridoxal phosphate)lysine
N74 (= N74) binding
GTGGT 178:182 (= GTGGT 179:183) binding
S266 (= S274) binding

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
55% identity, 96% coverage: 4:314/324 of query aligns to 3:306/306 of 2q3dA

query
sites
2q3dA

I

I

F

A

A

E

D

D

N

I

A

T

H

Q

S

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

R

I

L

N

R

R

R

I

V

A

T

P

D

R

G

G

A

V

V

T

A

-

D

I

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

G

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

N

A

M

M

I

L

W

Q

D

A

A

A

E

E

S

Q

T

A

G

G

K

L

L

I

K

K

P

P

G

D

M

T

T

I

I

I

V

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

G

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

Y

Y

K

R

L

C

L

V

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

V

L

L

L

K

R

A

A

L

Y

G

G

A

A

E

E

L

L

V

I

L

L

T

T

E

P

P

G

A

A

K

D

G

G

M

M

K

S

G

G

A

A

I

I

E

A

K

K

A

A

G

E

E

E

I

L

V

A

A

K

S

T

D

D

P

-

A

Q

T

R

Y

Y

F

F

M

V

P

P

A

Q

Q
|
Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

A

I

I

H

H

E

R

K

V

T

T

G

A

P

E

E

E

I

V

W

W

N

R

D

D

T

T

D

D

G

G

A

K

V

V

D

D

V

I

L

V

V

V

A

A

G

G

V
|
V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

Q

Y

V

I

I

K

K

N

E

I

-

A

-

G

R

K

K

P

P

I

S

L

A

S

R

-

F

V

V

A

A

V

V

E

E

P

P

I

A

V

A

S

S

P

P

V

V

I

L

T

S

Q

G

A

G

L

Q

A

K

G

G

E

-

I

-

K

-

P

-

S

-

P

P

H

H

K

P

I

I

Q

Q

G
|
G

I
|
I

G

G

A

A

G

G

F

F

V

V

P

P

K

P

N

V

L

L

D

D

L

Q

S

D

M

L

V

V

D

D

R

E

V

I

E

I

L

T

V

V

T

G

D

N

D

E

E

D

S

A

K

L

A

N

M

V

A

A

L

R

R

R

L

L

M

A

Q

R

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S
|
S

C

S

G

G

A

A

M

T

A

V

V

A

A

A

V

L

R

Q

L

V

A

A

E

R

K

R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

I

V

V

I

V

L

L

P
|
P

D
|
D

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

P

L

L

F

F

S

A

active site: K44 (= K44), S266 (= S274), P293 (= P301)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K44), T71 (= T71), S72 (= S72), N74 (= N74), T75 (= T75), Q144 (= Q145), V177 (= V178), G178 (= G179), T179 (= T180), G180 (= G181), T182 (= T183), G222 (= G230), I223 (= I231), S266 (= S274), P293 (= P301), D294 (= D302)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
54% identity, 97% coverage: 3:316/324 of query aligns to 2:311/318 of 4lmaA

query
sites
4lmaA

R

R

I

I

F

A

A

H

D

D

N

V

A

T

H

E

S

L

I

V

G

G

N

R

T

T

P

P

L

L

V

V

Q

Q

I

L

N

N

R

R

I

I

A

-

P

P

R

Q

G

A

V

E

-

G

-

C

-

L

-

G

T

R

I

I

L

V

A

M

K

K

I

L

E

E

G

G

R

M

N

N

P

P

G

A

Y

A

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

T

N

H

M

M

I

I

W

N

D

S

A

A

E

E

S

K

T

A

G

G

K

L

L

I

K

N

P

P

G

E

M

T

T

T

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

F

M

V

T

A

A

R

K

G

G

Y

Y

K

R

L

L

L

I

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

L

E

E

R

R

K

A

V

M

L

L

K

K

A

A

L

Y

G

G

A

A

E

T

L

L

V

E

L

L

T

T

E

P

P

G

A

S

K

Q

G

G

M

M

K

K

G

G

A

A

I

I

E

L

K

R

A

A

G

Q

E

Q

I

I

V

V

A

D

S

S

D

I

P

P

A

G

T

A

Y

Y

F

M

M

L

P

-

A

Q

Q

Q

F

F

E

R

N

N

P

P

A

S

N

N

P

P

A

E

I

I

H

H

E

R

K

L

T

T

G

A

P

E

E

E

I

I

W

W

N

Q

D

D

T

T

D

E

G

G

A

Q

V

V

D

D

V

F

L

I

V

V

A

A

G

G

V

V

G
|
G

T
|
T

G
|
G

G

G

T
|
T

I

I

T

T

G

G

V

V

S

A

R

E

Y

V

I

I

K

K

N

-

I

-

A

S

G

R

K

K

P

P

I

S

L

F

S

Q

V

V

-

V

A

A

V

V

E

E

P

P

I

F

V

N

S

S

P

P

V

V

I

I

T

S

Q

G

A

G

L

-

A

-

G

-

E

-

I

-

K

N

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

E

N

V

L

L

D

R

L

T

S

D

M

L

V

I

D

D

R

E

V

V

E

I

L

T

V

V

T

S

D

D

D

E

E

E

S

A

K

F

A

Q

M

F

A

G

L

R

R

R

L

L

M

A

Q

K

E

E

G

G

I

L

L

L

C

S

G

G

I

I

S
|
S

C

S

G

G

A

A

A

N

M

L

A

C

V

A

A

A

V

I

R

Q

L

L

A

A

E

Q

K

R

P

P

E

E

M

N

Q

E

G

G

K

K

T

L

I

I

V

V

I

I

L

Q

P
|
P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

L

L

M

F

F

S

Q

D

N

L

I

active site: K46 (= K44), S269 (= S274)
binding pyridoxal-5'-phosphate: K46 (= K44), N77 (= N74), G181 (= G179), T182 (= T180), G183 (= G181), T185 (= T183), G225 (= G230), S269 (= S274), P296 (= P301)

7n2tA O-acetylserine sulfhydrylase from citrullus vulgaris in the internal aldimine state, with citrate bound (see paper)
53% identity, 97% coverage: 2:316/324 of query aligns to 1:309/309 of 7n2tA

query
sites
7n2tA

S

S

R

T

I

I

F

A

A

K

D

D

N

V

A

T

H

E

S

L

I

I

G

G

N

N

T

T

P

P

L

L

V

V

Q

Y

I

L

N

N

R

R

I

V

A

V

P

D

R

G

G

C

V

V

T

A

-

R

I

V

L

A

A

A

K

K

I

L

E

E

G

M

R

M

N

E

P

P

G

C

Y

S

S

S

V

V

K

K

C

D

R

R

I

I

G

G

A

Y

N

S

M

M

I

I

W

S

D

D

A

A

E

E

S

N

T

K

G

G

K

L

L

I

K

T

P

P

G

G

M

E

T

S

I

V

-

L

V

I

E

E

P

P

T

T

S

S

G

G

N

N

T

T

G

G

I

I

G

G

L

L

A

A

F

F

V

I

A

A

R

K

G

G

Y

Y

K

R

L

L

L

I

T

C

M

M

P

P

A

A

S

S

M

M

S

S

I

L

E

E

R

R

K

T

V

I

L

L

K

R

A

A

L

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

E

D

P

P

A

A

K

R

G

G

M

M

K

K

G

G

A

A

I

V

E

Q

K

K

A

A

G

E

E

E

I

I

V

K

A

A

S

K

D

T

P

P

A

N

T

S

Y

Y

F

I

M

L

P

-

A

Q

Q

Q

F

F

E

E

N

N

P

P

A

A

N

N

P

P

A

K

I

I

H

H

E

Y

K

E

T

T

G

G

P

P

E

E

I

I

W

W

N

R

D

G

T

S

D

G

G

G

A

K

V

I

D

D

V

A

L

L

V

V

A

S

G

G

V

I

G

G

T

T

G

G

T

T

I

V

T

T

G

G

V

A

S

G

R

K

Y

Y

I

L

K

K

N

E

I

-

A

Q

G

N

K

P

P

N

I

I

L

K

S

L

V

Y

A

G

V

V

E

E

P

P

I

V

V

E

S

S

P

A

V

I

I

L

T

S

Q

G

A

G

L

-

A

-

G

-

E

-

I

-

K

K

P

P

S

G

P

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

K

G

N

V

L

L

D

D

L

V

S

N

M

L

V

L

D

D

R

E

V

V

E

I

L

Q

V

V

T

S

D

S

D

E

E

E

S

S

K

I

A

E

M

T

A

A

L

K

R

L

L

L

M

A

Q

L

E

K

E

E

G

G

I

L

L

L

C

V

G

G

I

I

S

S

C

S

G

G

A

A

M

A

A

A

V

A

A

A

V

I

R
|
R

L

I

A

A

E

K

K
x
R

P

P

E

E

M

N

Q

A

G

G

K

K

T

L

I

I

V

V

V

A

I

V

L

F

P

P

D

S

S

F

G

G

E

E

R

R

Y

Y

L

L

S

S

S

T

M

V

L

L

F

F

S

E

D

S

L

V

binding phosphate ion: R277 (= R284), R281 (≠ K288)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
55% identity, 94% coverage: 4:308/324 of query aligns to 3:300/300 of 3zeiA

query
sites
3zeiA

I

I

F

A

A

E

D

D

N

I

A

T

H

Q

S

L

I

I

G

G

N

R

T

T

P

P

L

L

V

V

Q

R

I

L

N

R

R

R

I

V

A

T

P

D

R

G

G

A

V

V

T

A

-

D

I

I

L

V

A

A

K

K

I

L

E

E

G

F

R

F

N

N

P

P

G

A

Y

N

S

S

V

V

K
|
K

C

D

R

R

I

I

G

G

A

V

N

A

M

M

I

L

W

Q

D

A

A

A

E

E

S

Q

T

A

G

G

K

L

L

I

K

K

P

P

G

D

M

T

T

I

I

I

V

L

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

F

M

V

V

A

C

A

A

R

R

G

G

Y

Y

K

R

L

C

L

V

L

L

T

T

M

M

P

P

A

E

S

T

M

M

S

S

I

L

E

E

R

R

K

M

V

L

L

L

K

R

A

A

L

Y

G

G

A

A

E

E

L

L

V

I

L

L

T

T

E

P

P

G

A

A

K

D

G

G

M

M

K

S

G

G

A

A

I
|
I

E

A

K

K

A

A

G

E

E

E

I

L

V

A

A

K

S

T

D

D

P

-

A

Q

T

R

Y

Y

F

F

M

V

P

P

A

Q

Q
|
Q

F
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active site: K44 (= K44), S266 (= S274), P293 (= P301)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T71), S72 (= S72), I126 (= I126), Q144 (= Q145), F145 (= F146), K215 (≠ A217), G222 (= G230), A225 (= A233), F227 (= F235)
binding pyridoxal-5'-phosphate: K44 (= K44), N74 (= N74), V177 (= V178), G178 (= G179), T179 (= T180), G180 (= G181), T182 (= T183), G222 (= G230), S266 (= S274), P293 (= P301), D294 (= D302)

Query Sequence

>GFF1476 FitnessBrowser__WCS417:GFF1476
MSRIFADNAHSIGNTPLVQINRIAPRGVTILAKIEGRNPGYSVKCRIGANMIWDAESTGK
LKPGMTIVEPTSGNTGIGLAFVAAARGYKLLLTMPASMSIERRKVLKALGAELVLTEPAK
GMKGAIEKAGEIVASDPATYFMPAQFENPANPAIHEKTTGPEIWNDTDGAVDVLVAGVGT
GGTITGVSRYIKNIAGKPILSVAVEPIVSPVITQALAGEEIKPSPHKIQGIGAGFVPKNL
DLSMVDRVELVTDDESKAMALRLMQEEGILCGISCGAAMAVAVRLAEKPEMQGKTIVVIL
PDSGERYLSSMLFSDLFTEQENQA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory