SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1491 FitnessBrowser__psRCH2:GFF1491 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 5 hits to proteins with known functional sites (download)

8gxfB Pseudomonas flexibilis gcn5 family acetyltransferase (see paper)
69% identity, 99% coverage: 2:186/186 of query aligns to 1:185/187 of 8gxfB

query
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8gxfB

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binding coenzyme a: T93 (= T94), H100 (= H101), G101 (= G102), K109 (= K110), T129 (= T130), R135 (= R136)

8gxkB Pseudomonas jinjuensis n-acetyltransferase (see paper)
68% identity, 99% coverage: 2:185/186 of query aligns to 1:184/188 of 8gxkB

query
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8gxkB

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binding coenzyme a: M40 (= M41), T93 (= T94), W94 (= W95), H100 (= H101), G101 (= G102), T129 (= T130), N133 (= N134), L134 (= L135), R135 (= R136), S136 (= S137), A139 (= A140), K142 (= K143)

1yreB Hypothetical protein pa3270 from pseudomonas aeruginosa in complex with coa
68% identity, 99% coverage: 2:185/186 of query aligns to 1:184/187 of 1yreB

query
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1yreB

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binding coenzyme a: M40 (= M41), T93 (= T94), W94 (= W95), H100 (= H101), G101 (= G102), N105 (= N106), R135 (= R136), G138 (≠ R139)

6c37A Mycobacterium smegmatis rimj in complex with coa-disulfide
26% identity, 67% coverage: 55:178/186 of query aligns to 72:194/209 of 6c37A

query
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6c37A

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binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: G110 (= G92), Y111 (≠ W93), W112 (= W95), V113 (≠ L96), T118 (≠ H101), G119 (= G102), G121 (= G104), A123 (≠ N106), T124 (≠ A107), T146 (≠ K129), N151 (= N134), T152 (≠ L135), P153 (≠ R136), S154 (= S137), V157 (≠ A140), H160 (≠ K143)

Sites not aligning to the query:

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: 42, 205

6c32A Mycobacterium smegmatis rimj with accoa
26% identity, 67% coverage: 55:178/186 of query aligns to 72:194/209 of 6c32A

query
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6c32A

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binding acetyl coenzyme *a: G110 (= G92), Y111 (≠ W93), W112 (= W95), V113 (≠ L96), T118 (≠ H101), G119 (= G102), G121 (= G104), A123 (≠ N106), T124 (≠ A107), T146 (≠ K129), N151 (= N134), T152 (≠ L135), P153 (≠ R136), S154 (= S137), V157 (≠ A140), H160 (≠ K143)

Sites not aligning to the query:

binding acetyl coenzyme *a: 205

Query Sequence

>GFF1491 FitnessBrowser__psRCH2:GFF1491
MFSPHPVTLQRGALRLAPLMEADIPELVALAERNRAELTFMNGPLRPDWYRHALAEQRDG
HAVVFSIRMAERLIGTTRFADFVSSLPAAELGWTWLDQAEHGTGLNASIKFLLLRHAFEE
WQLVRLQLKTAASNLRSQRAIEKLGAQREGVLRNHRRLADGRLDDTVLYSITDRDWPQVR
QCLAAE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory