SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1617 FitnessBrowser__Phaeo:GFF1617 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q88H32 Ornithine cyclodeaminase; OCD; EC 4.3.1.12 from Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440) (see paper)
53% identity, 97% coverage: 12:348/348 of query aligns to 4:342/350 of Q88H32

query
sites
Q88H32

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R45 (= R53) binding
K69 (= K77) binding
T84 (= T92) binding
R112 (= R120) binding ; binding
AQ 139:140 (= AQ 147:148) binding
D161 (= D169) binding
T202 (= T209) binding
VGGD 225:228 (≠ IGGD 232:235) binding
D228 (= D235) binding
K232 (= K239) binding
S293 (= S300) binding
V294 (= V301) binding
K331 (≠ R337) binding

1u7hA Structure and a proposed mechanism for ornithine cyclodeaminase from pseudomonas putida (see paper)
53% identity, 97% coverage: 12:348/348 of query aligns to 3:341/341 of 1u7hA

query
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1u7hA

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active site: E55 (= E64), D227 (= D235)
binding nicotinamide-adenine-dinucleotide: T83 (= T92), V84 (= V93), R111 (= R120), T112 (= T121), G135 (= G144), G137 (= G146), A138 (= A147), Q139 (= Q148), Y159 (= Y168), D160 (= D169), T161 (≠ V170), D162 (= D171), V200 (≠ C208), T201 (= T209), A202 (= A210), I209 (= I217), V224 (≠ I232), G225 (= G233), D227 (= D235), K231 (= K239), S292 (= S300), V293 (= V301), G294 (= G302)

1x7dA Crystal structure analysis of ornithine cyclodeaminase complexed with NAD and ornithine to 1.6 angstroms (see paper)
54% identity, 95% coverage: 12:340/348 of query aligns to 3:333/340 of 1x7dA

query
sites
1x7dA

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active site: E55 (= E64), D227 (= D235)
binding nicotinamide-adenine-dinucleotide: T83 (= T92), V84 (= V93), T108 (= T117), R111 (= R120), T112 (= T121), G135 (= G144), G137 (= G146), A138 (= A147), Q139 (= Q148), Y159 (= Y168), D160 (= D169), T161 (≠ V170), D162 (= D171), V200 (≠ C208), T201 (= T209), A202 (= A210), I209 (= I217), V224 (≠ I232), G225 (= G233), D227 (= D235), K231 (= K239), S292 (= S300), V293 (= V301), G294 (= G302)
binding L-ornithine: R44 (= R53), V53 (= V62), E55 (= E64), M57 (= M66), K68 (= K77), V70 (= V79), N71 (= N80), G72 (= G81), R111 (= R120), D227 (= D235), V293 (= V301), G294 (= G302)

O28608 Alanine dehydrogenase; AlaDH; EC 1.4.1.1 from Archaeoglobus fulgidus (strain ATCC 49558 / DSM 4304 / JCM 9628 / NBRC 100126 / VC-16) (see paper)
33% identity, 82% coverage: 31:316/348 of query aligns to 17:306/322 of O28608

query
sites
O28608

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C

V

K

Q

T

P

P

A

E

E

A

A

I

S

E

R

G

C

A

D

Q

V

I

L

L

V

T

T

C

T

T

P

A

S

D

R

K

K

Q

-

Y

-

A

-

T

P

I

V

L

V

T

K

D

A

N

E

M

W

V

V

G

E

S

E

G

G

V

T

H

H

I

I

N

N

A

A

I

I

G
|
G

G
x
A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

A

D

P

V

G

E

I

I

L

L

S

K

R

K

S

A

D

K

V

I

F

V

V

V

E

D

F

D

P

L

P

E

Q

Q

T

A

R

K

V

H

E

G

G

G

E

E

I

I

Q

N

Q

V

M

A

-

V

-

S

-

K

-

G

-

V

-

I

-

G

P

V

E

E

D

D

F

V

E

H

V

A

T

T

E

-

L

I

W

G

Q

E

V

V

I

I

L

A

G

G

Q

L

K

K

P

D

G

G

R

R

R

E

D

S

D

D

K

E

Q

E

I

I

T

T

L

I

F

F

D

D

S
|
S

V

T

G

G

F

L

A

A

I

I

E

Q

D

D

F

V

S

A

A

V

L

A

R

K

Y

V

I

V

R

Y

D

E

R108 (= R120) binding
TQ 135:136 (≠ AQ 147:148) binding
DVR 157:159 (≠ DVD 169:171) binding
GAD 217:219 (≠ GGD 233:235) binding
K223 (= K239) binding
S290 (= S300) binding

1omoA Alanine dehydrogenase dimer w/bound NAD (archaeal) (see paper)
33% identity, 82% coverage: 31:317/348 of query aligns to 17:307/320 of 1omoA

query
sites
1omoA

M

M

K

D

D

E

L

A

A

M

T

N

Y

A

I

V

E

E

D

A

F

F

K

R

R

L

W

Y

E

A

L

L

F

G

D

K

K

A

T

Q

-

M

-

P

P

P

R

K

V

V

A

Y

S

L

H

E

S

F

D

E

V

K

G

G

V

D

I

L

E
x
R

L

A

M

M

P

P

T

A

S

H

D

L

G

M

E

G

A

Y

Y

A

G

G

F

L

K

K

Y

W

V

V

N

N

G

S

H

H

P

P

K

G

N

N

T

P

S

D

E

K

G

G

L

L

Q

P

T

T

V

V

T

M

A

A

F

L

G

M

L

I

L

L

A

N

D

S

V

P

Y

E

T

T

G

G

Y

F

P

P

V

L

L

A

L

V

T

M

E

D

M

A

T

T

I

Y

L

T

T

T

A

S

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

G

A

G

M

I

V

A

A

A

K

K

H

Y

L

L

A

A

P

R

K

K

G

N

A

S

T

S

T

V

M

F

A

G

M

F

I

I

G
|
G

N
x
C

G
|
G

A
x
T

Q
|
Q

S

A

E

Y

F

F

Q

Q

S

L

L

E

A

A

M

L

Q

R

A

R

I

V

C

F

G

D

L

I

K

G

S

E

V

V

R

K

L

A

Y
|
Y

D
|
D

V
|
V

D
x
R

P

E

A

K

A

A

T

A

A

K

K

K

C

F

A

V

A

S

N

Y

L

C

A

E

G

D

T

R

G

G

L

I

A

S

V

-

V

A

S

S

C

V

K

Q

T

P

P

A

E

E

A

A

I

S

E

R

G

C

A

D

Q

V

I

L

L

V

T

T

T
|
T

C
x
T

T
x
P

A

S

D

R

K

K

Q

-

Y

-

A

-

T

P

I
x
V

L

V

T

K

D

A

N

E

M

W

V

V

G

E

S

E

G

G

V

T

H

H

I

I

N

N

A

A

I
|
I

G
|
G

G

A

D
|
D

C

G

P

P

G

G

K
|
K

T

Q

E

E

L

L

A

D

P

V

G

E

I

I

L

L

S

K

R

K

S

A

D

K

V

I

F

V

V

V

E

D

F

D

P

L

P

E

Q

Q

T

A

R

K

V

H

E

G

G

G

E

E

I

I

Q

N

Q

V

M

A

-

V

-

S

-

K

-

G

-

V

-

I

-

G

P

V

E

E

D

D

F

V

E

H

V

A

T

T

E

-

L

I

W

G

Q

E

V

V

I

I

L

A

G

G

Q

L

K

K

P

D

G

G

R

R

R

E

D

S

D

D

K

E

Q

E

I

I

T

T

L

I

F

F

D

D

S
|
S

V
x
T

G
|
G

F

L

A

A

I

I

E

Q

D

D

F

V

S

A

A

V

L

A

R

K

Y

V

I

V

R

Y

D

E

R

N

active site: R52 (≠ E64), D219 (= D235)
binding nicotinamide-adenine-dinucleotide: R108 (= R120), T109 (= T121), G132 (= G144), C133 (≠ N145), G134 (= G146), T135 (≠ A147), Q136 (= Q148), Y156 (= Y168), D157 (= D169), V158 (= V170), R159 (≠ D171), T194 (= T207), T195 (≠ C208), P196 (≠ T209), V201 (≠ I217), I216 (= I232), G217 (= G233), D219 (= D235), K223 (= K239), S290 (= S300), T291 (≠ V301), G292 (= G302)

6t3eB Structure of thermococcus litoralis delta(1)-pyrroline-2-carboxylate reductase in complex with nadh and l-proline (see paper)
30% identity, 80% coverage: 31:307/348 of query aligns to 15:303/325 of 6t3eB

query
sites
6t3eB

M

M

K

K

D

E

L

V

A

I

T

E

Y

S

I

V

E

E

E

R

D

A

F

F

K

-

R

-

W

L

E

E

L

L

F

Y

D

N

K

G

T

K

P

A

R

K

V

V

A

P

S

L

H
x
R

S

T

D

I

V

I

-

E

-

V

-

E

-

K

-

H

-

N

G

G

V

F

I

I

E
x
L

L

Y

M
|
M

P

P

T

S

-

Y

-

L

S

E

D

D

G

S

E

E

A

A

Y

L

G

A

F

V

K
|
K

Y

V

V
|
V

N

S

G
x
L

H

Y

P

P

K

E

N

N

T

T

S

K

E

K

G

G

L

L

Q

P

T
x
S

V
|
V

T

L

A

A

F

S

G

I

L

L

A

N

D

D

V

P

Y

K

T

T

G

G

Y

A

P

P

V

L

L

A

L

L

T

M

E

E

M

G

T

T

I

F

L

I

T
|
T

A

A

L

M

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

G

M

V

V

A

A

T

K

K

H

Y

L

L

A

A

P

R

K

K

G

D

A

S

T

K

T

I

M

A

A

G

M

I

I

I

G
|
G

N
x
A

G
|
G

A
x
V

Q
|
Q

S

A

E

R

F

T

Q

Q

S

L

L

W

A

A

M

V

Q

C

A

E

I

V

C

R

G

N

L

I

K

E

S

K

V

A

R

L

L

V

Y
|
Y

D
|
D

V
x
I

D

N

P

P

A

K

A
x
N

T

A

A

K

K

K

C

F

A

A

A

E

N

E

L

M

A

S

G

K

T

K

-

L

G

G

L

I

A

E

V

I

V

K

S

T

C

V

K

E

T

S

P

A

E

R

E

E

A

A

I

T

E

E

G

K

A

S

Q

D

I

I

L

L

T

I

T

V

C
x
A

T
|
T

A
x
T

D
x
A

K

R

Q

E

Y

-

A

-

T

P

I
x
V

L

V

T

K

D

G

N

G

M

W

V

I

G

R

S

E

G

G

V

T

H

H

I

I

N

N

A

S

I
x
V

G
|
G

-
x
W

-

V

-

G

-

R

-

D

-

A

-

R

-

E

-

L

-

D

G

S

D

E

C

T

P

V

G

R

K

K

T

S

E

K

L

L

A

V

-

V

-

D

-

S

-

K

P

E

G

G

I

V

L

L

S

N

R

E

S

S

-

G

D

D

V

I

F

I

V

I

E

P

F

M

P

K

P

E

Q

G

T

V

R

I

V

D

E

E

G

G

E

H

I

I

Q

H

Q

-

M

-

P

-

E

-

D

-

F

-

E

-

V

-

T

A

E

E

L

L

W

A

Q

E

V

I

I

V

L

A

G

G

Q

V

K

K

P

K

G

G

R

R

R

E

D

N

K

R

Q

E

I

I

T

T

L

L

F

F

D

K

S
|
S

V
|
V

G
|
G

F

L

A

A

I

I

E

E

D

D

binding 1,4-dihydronicotinamide adenine dinucleotide: L71 (≠ G81), S82 (≠ T92), V83 (= V93), T107 (= T117), R110 (= R120), T111 (= T121), G134 (= G144), A135 (≠ N145), G136 (= G146), V137 (≠ A147), Q138 (= Q148), Y158 (= Y168), D159 (= D169), I160 (≠ V170), N164 (≠ A174), A199 (≠ C208), T200 (= T209), T201 (≠ A210), A202 (≠ D211), V206 (≠ I217), V221 (≠ I232), G222 (= G233), W223 (vs. gap), S296 (= S300), V297 (= V301), G298 (= G302)
binding proline: R39 (≠ H57), L52 (≠ E64), M54 (= M66), K67 (= K77), V69 (= V79), V83 (= V93), R110 (= R120), W223 (vs. gap), V297 (= V301)

5yu4A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
32% identity, 81% coverage: 61:342/348 of query aligns to 60:343/344 of 5yu4A

query
sites
5yu4A

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

T

H

S

R

D

E

-

P

G

G

E

D

A

H

Y

I

G

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

S

A

E

R

-

F

G

G

L

L

Q

P

T
|
T

V
x
I

T

L

A

G

F

T

G

V

L

A

L

R

A

Y

D

D

V

D

Y

T

T

T

G

G

Y

A

P

L

V

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

M

V

V

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

G

D

A

S

T

H

T

T

M

L

A

G

M

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

S

L

L

H

A

A

M

L

Q

S

A

L

I

V

C

L

G

P

L

L

K

Q

S

R

V

A

R

L

L

V

Y

W

D
|
D

V
x
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

C

F

A

A

A

R

N

R

L

A

A

A

G

F

T

T

G

G

L

V

A

S

V

V

V

E

S

I

C

A

K

E

T

P

P

A

E

R

E

I

A

A

I

A

E

E

G

A

A

D

Q

V

I

I

L

S

T

T

T
x
A

C
x
T

T
x
S

A
x
V

D

A

K

V

Q

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

N

T

M

G

V

V

G

R

S

E

G

H

V

L

H

H

I

I

N

N

A

A

I
x
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

A

P

P

L

G

G

I

L

L

L

S

E

R

R

S

A

D

F

V

V

F

T

V

A

E

D

F

H

P

P

P

E

Q

Q

T

A

R

L

V

R

E

E

G

G

E

E

I

C

Q

Q

M

L

P

S

E

A

D

D

F

R

E

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

L

A

-

D

-

P

G

A

Q

A

K

A

P

A

G

G

R

R

R

Q

D

D

D

-

K

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

I

F

R

L

D

E

R

A

I

A

K

A

D

E

T

R

D

D

F

L

F

G

L

I

E

R

L

V

D

G

M

I

L

E

A

H

D

H

P

P

D

G

D

D

P

A

R

L

D

D

L

P

F

Y

G

A

M

L

binding 2,4-diaminobutyric acid: E63 (= E64), M65 (= M66), K77 (= K77), R121 (= R120), A235 (≠ G234), D236 (= D235), T302 (≠ V301), G303 (= G302)
binding nicotinamide-adenine-dinucleotide: Y81 (≠ G81), T93 (= T92), I94 (≠ V93), T118 (= T117), R121 (= R120), T122 (= T121), I144 (= I143), G145 (= G144), G147 (= G146), A148 (= A147), Q149 (= Q148), D170 (= D169), T171 (≠ V170), D172 (= D171), H175 (≠ A174), A208 (≠ T207), T209 (≠ C208), S210 (≠ T209), V211 (≠ A210), V218 (≠ I217), V233 (≠ I232), G234 (= G233), A235 (≠ G234), D236 (= D235), K240 (= K239), S301 (= S300), T302 (≠ V301), G303 (= G302)

Sites not aligning to the query:

binding 2,4-diaminobutyric acid: 49

5yu3A Structural basis for recognition of l-lysine, l-ornithine, and l-2,4- diamino butyric acid by lysine cyclodeaminase (see paper)
32% identity, 81% coverage: 61:342/348 of query aligns to 60:343/344 of 5yu3A

query
sites
5yu3A

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

T

H

S

R

D

E

-

P

G

G

E

D

A

H

Y

I

G

T

F

L

K
|
K

Y

T

V
|
V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

S

A

E

R

-

F

G

G

L

L

Q

P

T
|
T

V
x
I

T

L

A

G

F

T

G

V

L

A

L

R

A

Y

D

D

V

D

Y

T

T

T

G

G

Y

A

P

L

V

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

M

V

V

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

G

D

A

S

T

H

T

T

M

L

A

G

M

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

S

L

L

H

A

A

M

L

Q

S

A

L

I

V

C

L

G

P

L

L

K

Q

S

R

V

A

R

L

L

V

Y

W

D
|
D

V
x
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

C

F

A

A

A

R

N

R

L

A

A

A

G

F

T

T

G

G

L

V

A

S

V

V

V

E

S

I

C

A

K

E

T

P

P

A

E

R

E

I

A

A

I

A

E

E

G

A

A

D

Q

V

I

I

L

S

T

T

T
x
A

C
x
T

T
x
S

A
x
V

D

A

K

V

Q

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

N

T

M

G

V

V

G

R

S

E

G

H

V

L

H

H

I

I

N

N

A

A

I
x
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

A

P

P

L

G

G

I

L

L

L

S

E

R

R

S

A

D

F

V

V

F

T

V

A

E

D

F

H

P

P

P

E

Q

Q

T

A

R

L

V

R

E

E

G

G

E

E

I

C

Q

Q

M

L

P

S

E

A

D

D

F

R

E

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

L

A

-

D

-

P

G

A

Q

A

K

A

P

A

G

G

R

R

R

Q

D

D

D

-

K

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

I

F

R

L

D

E

R

A

I

A

K

A

D

E

T

R

D

D

F

L

F

G

L

I

E

R

L

V

D

G

M

I

L

E

A

H

D

H

P

P

D

G

D

D

P

A

R

L

D

D

L

P

F

Y

G

A

M

L

binding nicotinamide-adenine-dinucleotide: Y81 (≠ G81), T93 (= T92), I94 (≠ V93), T118 (= T117), R121 (= R120), T122 (= T121), I144 (= I143), G145 (= G144), G147 (= G146), A148 (= A147), Q149 (= Q148), D170 (= D169), T171 (≠ V170), D172 (= D171), H175 (≠ A174), A208 (≠ T207), T209 (≠ C208), S210 (≠ T209), V211 (≠ A210), V218 (≠ I217), V233 (≠ I232), G234 (= G233), A235 (≠ G234), D236 (= D235), K240 (= K239), S301 (= S300), T302 (≠ V301), G303 (= G302)
binding proline: E63 (= E64), M65 (= M66), K77 (= K77), V79 (= V79), R121 (= R120), A235 (≠ G234), T302 (≠ V301), G303 (= G302)

Sites not aligning to the query:

binding proline: 49

5gziA Cyclodeaminase_pa
32% identity, 81% coverage: 61:342/348 of query aligns to 64:347/354 of 5gziA

query
sites
5gziA

G

G

V

I

I

W

E
|
E

L

W

M
|
M

P

P

T

H

S

R

D

E

-

P

G

G

E

D

A

H

Y

I

G

T

F

L

K
|
K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

S

A

E

R

-

F

G

G

L

L

Q

P

T
|
T

V
x
I

T

L

A

G

F

T

G

V

L

A

L

R

A

Y

D

D

V

D

Y

T

T

T

G

G

Y

A

P

L

V

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

M

V

V

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

G

D

A

S

T

H

T

T

M

L

A

G

M

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

S

L

L

H

A

A

M

L

Q

S

A

L

I

V

C

L

G

P

L

L

K

Q

S

R

V

A

R

L

L

V

Y

W

D
|
D

V
x
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

C

F

A

A

A

R

N

R

L

A

A

A

G

F

T

T

G

G

L

V

A

S

V

V

V

E

S

I

C

A

K

E

T

P

P

A

E

R

E

I

A

A

I

A

E

E

G

A

A

D

Q

V

I

I

L

S

T

T

T
x
A

C
x
T

T
x
S

A
x
V

D

A

K

V

Q

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

N

T

M

G

V

V

G

R

S

E

G

H

V

L

H

H

I

I

N

N

A

A

I
x
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

A

P

P

L

G

G

I

L

L

L

S

E

R

R

S

A

D

F

V

V

F

T

V

A

E

D

F

H

P

P

P

E

Q

Q

T

A

R

L

V

R

E

E

G

G

E

E

I

C

Q

Q

M

L

P

S

E

A

D

D

F

R

E

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

L

A

-

D

-

P

G

A

Q

A

K

A

P

A

G

G

R

R

R

Q

D

D

D

-

K

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

I

F

R

L

D

E

R

A

I

A

K

A

D

E

T

R

D

D

F

L

F

G

L

I

E

R

L

V

D

G

M

I

L

E

A

H

D

H

P

P

D

G

D

D

P

A

R

L

D

D

L

P

F

Y

G

A

M

L

binding nicotinamide-adenine-dinucleotide: Y85 (≠ G81), T97 (= T92), I98 (≠ V93), T122 (= T117), R125 (= R120), T126 (= T121), I148 (= I143), G149 (= G144), G151 (= G146), A152 (= A147), Q153 (= Q148), D174 (= D169), T175 (≠ V170), D176 (= D171), H179 (≠ A174), A212 (≠ T207), T213 (≠ C208), S214 (≠ T209), V215 (≠ A210), V222 (≠ I217), V237 (≠ I232), G238 (= G233), A239 (≠ G234), D240 (= D235), K244 (= K239), S305 (= S300), T306 (≠ V301), G307 (= G302)
binding (2S)-piperidine-2-carboxylic acid: E67 (= E64), M69 (= M66), K81 (= K77), R125 (= R120), A239 (≠ G234), T306 (≠ V301), G307 (= G302)

Sites not aligning to the query:

binding (2S)-piperidine-2-carboxylic acid: 53

5gzlA Cyclodeaminase_pa
32% identity, 81% coverage: 61:342/348 of query aligns to 64:347/357 of 5gzlA

query
sites
5gzlA

G

G

V
x
I

I

W

E
|
E

L

W

M

M

P

P

T

H

S

R

D

E

-

P

G

G

E

D

A

H

Y

I

G

T

F

L

K

K

Y

T

V

V

N

G

G
x
Y

H

S

P

P

K

A

N

N

T

P

S

A

E

R

-

F

G

G

L

L

Q

P

T
|
T

V
x
I

T

L

A

G

F

T

G

V

L

A

L

R

A

Y

D

D

V

D

Y

T

T

T

G

G

Y

A

P

L

V

T

L

A

L

L

T

M

E

D

M

G

T

V

I

L

L

L

T
|
T

A

A

L

L

R
|
R

T
|
T

A

G

A

A

T

A

S

S

A

A

M

V

V

A

A

S

K

R

H

L

L

L

A

A

P

R

K

P

G

D

A

S

T

H

T

T

M

L

A

G

M

L

I
|
I

G
|
G

N

T

G
|
G

A
|
A

Q
|
Q

S

A

E

V

F

T

Q

Q

S

L

L

H

A

A

M

L

Q

S

A

L

I

V

C

L

G

P

L

L

K

Q

S

R

V

A

R

L

L

V

Y

W

D
|
D

V
x
T

D
|
D

P

P

A

A

A
x
H

T

R

A

E

K

S

C

F

A

A

A

R

N

R

L

A

A

A

G

F

T

T

G

G

L

V

A

S

V

V

V

E

S

I

C

A

K

E

T

P

P

A

E

R

E

I

A

A

I

A

E

E

G

A

A

D

Q

V

I

I

L

S

T

T

T
x
A

C
x
T

T
x
S

A
x
V

D

A

K

V

Q

G

Y

Q

A

G

T

P

I
x
V

L

L

T

P

D

D

N

T

M

G

V

V

G

R

S

E

G

H

V

L

H

H

I

I

N

N

A

A

I
x
V

G
|
G

G
x
A

D
|
D

C

L

P

V

G

G

K
|
K

T

T

E

E

L

L

A

P

P

L

G

G

I

L

L

L

S

E

R

R

S

A

D

F

V

V

F

T

V

A

E

D

F

H

P

P

P

E

Q

Q

T

A

R

L

V
x
R

E
|
E

G

G

E

E

I

C

Q

Q

M

L

P

S

E

A

D

D

F

R

E

L

V

G

T

P

E

Q

L

L

W

A

Q

H

V

L

I

C

L

A

-

D

-

P

G

A

Q

A

K

A

P

A

G

G

R

R

R

Q

D

D

D

-

K

-

Q

T

I

L

T

S

L

V

F

F

D

D

S
|
S

V
x
T

G
|
G

F

F

A

A

I

F

E

E

D

D

F

A

S

L

A

A

L

M

R

E

Y

V

I

F

R

L

D

E

R

A

I

A

K

A

D

E

T

R

D

D

F

L

F

G

L

I

E

R

L

V

D

G

M

I

L

E

A

H

D

H

P

P

D

G

D

D

P

A

R

L

D

D

L

P

F

Y

G

A

M

L

binding lysine: I65 (≠ V62), E67 (= E64), D240 (= D235), R267 (≠ V262), E268 (= E263)
binding nicotinamide-adenine-dinucleotide: Y85 (≠ G81), T97 (= T92), I98 (≠ V93), T122 (= T117), R125 (= R120), T126 (= T121), I148 (= I143), G149 (= G144), G151 (= G146), A152 (= A147), Q153 (= Q148), D174 (= D169), T175 (≠ V170), D176 (= D171), H179 (≠ A174), A212 (≠ T207), T213 (≠ C208), S214 (≠ T209), V215 (≠ A210), V222 (≠ I217), V237 (≠ I232), G238 (= G233), A239 (≠ G234), D240 (= D235), K244 (= K239), S305 (= S300), T306 (≠ V301), G307 (= G302)

Sites not aligning to the query:

binding lysine: 53, 54, 55, 57

6rqaB Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
31% identity, 73% coverage: 75:329/348 of query aligns to 67:322/322 of 6rqaB

query
sites
6rqaB

G

G

F

L

K

K

Y

A

V

G

N

G

G

Y

H

W

P

P

K
x
N

N

N

T

Q

S

K

E

H

G

N

L

L

Q

I

T

N

V

H

T

Q

A

S

F

T

G

V

L

F

L

L

A

F

D

D

V

P

Y

D

T

T

G

G

Y

R

P

V

V

S

L

A

T

V

E

G

M

G

T

N

I

L

L

L

T

T

A

A

L

L

R
|
R

T
|
T

A

A

T

A

S

S

A

A

M

V

V

S

A

I

K

K

H

Y

L

L

A

A

P

P

K

K

G

G

A

A

T

K

T

V

M

L

A

G

M

M

I

I

G
|
G

N

A

G
|
G

A
x
H

Q
|
Q

S

S

E

A

F

F

Q

Q

S

M

L

R

A

A

M

A

Q

A

A

N

I

V

C

H

G

R

L

F

K

E

S

K

V

V

R

I

L

G

Y

W

D
x
N

V
x
P

D
x
H

P

P

A

E

A

M

T

L

A

S

K

R

C

L

A

A

A

D

N

T

L

A

A

A

G

E

T

L

G

G

L

L

A

-

V

-

S

-

C

-

K

-

T

-

P

P

E

F

E

E

A

A

I

V

E

E

-

L

-

D

-

R

-

L

G

G

A

A

Q

E

I

A

L

D

T

V

T

I

C

V

T

S

A
x
I

D
x
T

K
x
S

Q
x
S

Y

F

A

S

T

P

I

L

L

L

T

M

D

N

N

E

M

H

V

V

G

K

S

G

G

P

V

T

H

H

I

I

N

A

A

A

I
x
M

G
|
G

G

T

D
|
D

C

T

P

K

G

G

K
|
K

T

Q

E

E

L

L

A

D

P

P

G

A

I

L

L

V

S

A

R

R

S

A

D

R

V

I

F

F

V

T

E

D

F

E

P

V

P

A

Q

Q

T

S

R

V

V

S

E

I

G

G

E

E

I

C

Q

Q

Q

H

-

A

-

I

-

A

-

G

-

L

M

I

P

R

E

E

D

D

F

-

E

Q

V

V

T

G

E

E

L

L

W

G

Q

A

V

V

I

V

L

A

G

G

Q

D

K

D

P

P

G

G

R

-

R

R

D

G

D

D

K

A

Q

E

I

V

T

T

L

I

F

F

D

D

S
x
G

V
x
T

G
|
G

F

V

A

G

I

L

E

Q

D

D

F

L

S

A

A

V

L

A

R

Q

Y

A

I

V

R

V

D

E

R

L

I

A

K

K

D

H

T

K

D

G

F

V

F

A

L

Q

E

E

L

V

D

E

M

I

binding Tb-Xo4: N76 (≠ K84)
binding nicotinamide-adenine-dinucleotide: R112 (= R120), T113 (= T121), G136 (= G144), G138 (= G146), H139 (≠ A147), Q140 (= Q148), N161 (≠ D169), P162 (≠ V170), H163 (≠ D171), I199 (≠ A210), T200 (≠ D211), S201 (≠ K212), S202 (≠ Q213), M221 (≠ I232), G222 (= G233), D224 (= D235), K228 (= K239), G293 (≠ S300), T294 (≠ V301), G295 (= G302)

Sites not aligning to the query:

binding Tb-Xo4: 48, 49, 50

6rqaA Crystal structure of the iminosuccinate reductase of paracoccus denitrificans in complex with NAD+ (see paper)
31% identity, 73% coverage: 75:329/348 of query aligns to 67:322/322 of 6rqaA

query
sites
6rqaA

G

G

F

L

K

K

Y

A

V

G

N

G

G

Y

H

W

P

P

K

N

N

N

T

Q

S

K

E

H

G

N

L

L

Q

I

T

N

V
x
H

T

Q

A

S

F

T

G

V

L

F

L

L

A

F

D

D

V

P

Y

D

T

T

G

G

Y

R

P

V

V

S

L

A

T

V

E

G

M

G

T

N

I

L

L

L

T

T

A

A

L

L

R

R

T
|
T

A

A

T

A

S

S

A

A

M

V

V

S

A

I

K

K

H

Y

L

L

A

A

P

P

K

K

G

G

A

A

T

K

T

V

M

L

A

G

M

M

I

I

G

G

N

A

G
|
G

A
x
H

Q
|
Q

S

S

E

A

F

F

Q

Q

S

M

L

R

A

A

M

A

Q

A

A

N

I

V

C

H

G

R

L

F

K

E

S

K

V

V

R

I

L

G

Y

W

D
x
N

V
x
P

D
x
H

P

P

A

E

A
x
M

T

L

A

S

K

R

C

L

A

A

A

D

N

T

L

A

A

A

G

E

T

L

G

G

L

L

A

-

V

-

S

-

C

-

K

-

T

-

P

P

E

F

E

E

A

A

I

V

E

E

-

L

-

D

-

R

-

L

G

G

A

A

Q

E

I

A

L

D

T

V

T

I

C

V

T

S

A
x
I

D
x
T

K
x
S

Q
x
S

Y

F

A

S

T

P

I

L

L

L

T

M

D

N

N

E

M

H

V

V

G

K

S

G

G

P

V

T

H

H

I

I

N

A

A

A

I
x
M

G
|
G

G

T

D
|
D

C

T

P

K

G

G

K
|
K

T

Q

E

E

L

L

A

D

P

P

G

A

I

L

L

V

S

A

R

R

S

A

D

R

V

I

F

F

V

T

E

D

F

E

P

V

P

A

Q

Q

T

S

R

V

V

S

E

I

G

G

E

E

I

C

Q

Q

Q

H

-

A

-

I

-

A

-

G

-

L

M

I

P

R

E

E

D

D

F

-

E

Q

V

V

T

G

E

E

L

L

W

G

Q

A

V

V

I

V

L

A

G

G

Q

D

K

D

P

P

G

G

R

-

R

R

D

G

D

D

K

A

Q

E

I

V

T

T

L

I

F

F

D

D

S
x
G

V
x
T

G
|
G

F

V

A

G

I

L

E

Q

D

D

F

L

S

A

A

V

L

A

R

Q

Y

A

I

V

R

V

D

E

R

L

I

A

K

K

D

H

T

K

D

G

F

V

F

A

L

Q

E

E

L

V

D

E

M

I

binding nicotinamide-adenine-dinucleotide: H85 (≠ V93), T113 (= T121), G138 (= G146), H139 (≠ A147), Q140 (= Q148), N161 (≠ D169), P162 (≠ V170), H163 (≠ D171), M166 (≠ A174), I199 (≠ A210), T200 (≠ D211), S201 (≠ K212), S202 (≠ Q213), M221 (≠ I232), G222 (= G233), D224 (= D235), K228 (= K239), G293 (≠ S300), T294 (≠ V301), G295 (= G302)

A1B8Z0 Iminosuccinate reductase; EC 1.4.1.- from Paracoccus denitrificans (strain Pd 1222) (see paper)
31% identity, 73% coverage: 75:329/348 of query aligns to 65:320/320 of A1B8Z0

query
sites
A1B8Z0

G

G

F

L

K

K

Y

A

V

G

N

G

G

Y

H

W

P

P

K

N

N

N

T

Q

S

K

E

H

G

N

L

L

Q

I

T

N

V

H

T

Q

A

S

F

T

G

V

L

F

L

L

A

F

D

D

V

P

Y

D

T

T

G

G

Y

R

P

V

V

S

L

A

T

V

E

G

M

G

T

N

I

L

L

L

T

T

A

A

L

L

R
|
R

T

T

A

A

T

A

S

S

A

A

M

V

V

S

A

I

K

K

H

Y

L

L

A

A

P

P

K

K

G

G

A

A

T

K

T

V

M

L

A

G

M

M

I

I

G

G

N

A

G

G

A
x
H

Q
|
Q

S

S

E

A

F

F

Q

Q

S

M

L

R

A

A

M

A

Q

A

A

N

I

V

C

H

G

R

L

F

K

E

S

K

V

V

R

I

L

G

Y

W

D
x
N

V

P

D

H

P

P

A

E

A

M

T

L

A

S

K

R

C

L

A

A

A

D

N

T

L

A

A

A

G

E

T

L

G

G

L

L

A

-

V

-

S

-

C

-

K

-

T

-

P

P

E

F

E

E

A

A

I

V

E

E

-

L

-

D

-

R

-

L

G

G

A

A

Q

E

I

A

L

D

T

V

T

I

C

V

T

S

A

I

D

T

K
x
S

Q

S

Y

F

A

S

T

P

I

L

L

L

T

M

D

N

N

E

M

H

V

V

G

K

S

G

G

P

V

T

H

H

I

I

N

A

A

A

I
x
M

G
|
G

G
x
T

D
|
D

C

T

P

K

G

G

K
|
K

T

Q

E

E

L

L

A

D

P

P

G

A

I

L

L

V

S

A

R

R

S

A

D

R

V

I

F

F

V

T

E

D

F

E

P

V

P

A

Q

Q

T

S

R

V

V

S

E

I

G

G

E

E

I

C

Q

Q

Q

H

-

A

-

I

-

A

-

G

-

L

M

I

P

R

E

E

D

D

F

-

E

Q

V

V

T

G

E

E

L

L

W

G

Q

A

V

V

I

V

L

A

G

G

Q

D

K

D

P

P

G

G

R

-

R

R

D

G

D

D

K

A

Q

E

I

V

T

T

L

I

F

F

D

D

S
x
G

V

T

G

G

F

V

A

G

I

L

E

Q

D

D

F

L

S

A

A

V

L

A

R

Q

Y

A

I

V

R

V

D

E

R

L

I

A

K

K

D

H

T

K

D

G

F

V

F

A

L

Q

E

E

L

V

D

E

M

I

R110 (= R120) binding
HQ 137:138 (≠ AQ 147:148) binding
N159 (≠ D169) binding
S199 (≠ K212) binding
MGTD 219:222 (≠ IGGD 232:235) binding
K226 (= K239) binding
G291 (≠ S300) binding

4mp6A Staphyloferrin b precursor biosynthetic enzyme sbnb bound to citrate and NAD+ (see paper)
24% identity, 76% coverage: 66:329/348 of query aligns to 62:333/334 of 4mp6A

query
sites
4mp6A

M

M

P

P

T

S

S

H

D

I

G

G

E

G

A

E

Y

H

-

A

-

I

-

S

G

G

F

I

K

K

Y

W

V

I

N

G

G

S

H

K

P

H

K

D

N

N

T

P

S

S

E

K

-

R

G

N

L

M

Q

E

T
x
R

V

A

T

S

A

G

F

V

G

I

L

I

L

L

A

N

D

D

V

P

Y

E

T

T

G

N

Y

Y

P

P

V

I

L

A

L

V

T

M

E

E

M

A

T

S

I

L

L

I

T

S

A

S

L

M

R
|
R

T
|
T

A

A

T

V

S

S

A

V

M

I

V

A

A

A

K

K

H

H

L

L

A

A

P

K

K

K

G

G

A

F

T

K

T

D

M

L

A

T

M

I

I

I

G
|
G

N
x
C

G
|
G

-
x
L

-
x
I

A

G

Q

D

S

K

E

Q

F

L

Q

Q

S

S

L

M

A

-

M

L

Q

E

A

Q

I

F

C

D

G

H

L

I

K

E

S

R

V

V

R

F

L

V

Y
|
Y

D
|
D

V
x
Q

D

F

P

S

A

E

A

A

T

C

A

A

K

R

C

F

A

V

A

D

N

R

L

W

A

Q

G

Q

T

Q

G

R

-

P

-

E

L

I

A

N

V

F

V

I

S

A

C

T

K

E

T

N

P

A

E

K

E

E

A

A

I

V

E

S

G

N

A

G

Q

E

I

V

L

V

T

I

T

T

C
|
C

T
|
T

A
x
V

D
x
T

K

D

Q

Q

Y

-

A

-

T

P

I
x
Y

L

I

T

E

D

Y

N

D

M

W

V

L

G

Q

S

K

G

G

V

A

H

F

I

I

N

S

A

N

I
|
I

G

-

D

-

C

-

P

-

G
x
S

K

I

T
x
M

E

D

L

V

A

H

P

K