SitesBLAST
Comparing GFF1621 FitnessBrowser__WCS417:GFF1621 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
7c3bC Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
31% identity, 94% coverage: 16:325/329 of query aligns to 21:333/334 of 7c3bC
P0DPQ8 Aromatic O-demethylase, reductase subunit; NADH--hemoprotein reductase; EC 1.6.2.- from Amycolatopsis sp. (strain ATCC 39116 / 75iv2) (see paper)
32% identity, 96% coverage: 15:329/329 of query aligns to 15:334/334 of P0DPQ8
5ogxA Crystal structure of amycolatopsis cytochrome p450 reductase gcob. (see paper)
32% identity, 96% coverage: 15:329/329 of query aligns to 14:333/333 of 5ogxA
- binding flavin-adenine dinucleotide: Y132 (= Y134), R144 (= R145), Q145 (≠ S146), Y146 (= Y147), S147 (= S148), H161 (= H162), V162 (≠ I163), V165 (= V166), G168 (= G169), V169 (= V170), A170 (≠ M171), T171 (≠ S172), T214 (= T211), F329 (= F325), P331 (≠ S327), S333 (≠ N329)
- binding fe2/s2 (inorganic) cluster: C34 (= C35), N35 (≠ R36), G37 (= G38), C39 (= C40), G40 (= G41), C42 (= C43), C74 (= C74)
7c3aA Ferredoxin reductase in carbazole 1,9a-dioxygenase (see paper)
30% identity, 94% coverage: 16:325/329 of query aligns to 20:320/321 of 7c3aA
- binding flavin-adenine dinucleotide: C39 (= C35), S41 (≠ T37), W60 (≠ Q56), R152 (= R145), A153 (≠ S146), Y154 (= Y147), S155 (= S148), Y169 (≠ H162), I170 (= I163), G176 (= G169), R177 (≠ V170), F178 (≠ M171), S179 (= S172), A218 (≠ T211), F320 (= F325)
- binding fe2/s2 (inorganic) cluster: Y37 (= Y33), C39 (= C35), A40 (≠ R36), G42 (= G38), C44 (= C40), G45 (= G41), C47 (= C43), C80 (= C74)
Sites not aligning to the query:
Q03304 Toluene-4-monooxygenase system, ferredoxin--NAD(+) reductase component; T4MO; Ferredoxin--NAD(+) reductase; Toluene-4-monooxygenase systme, electron transfer component; EC 1.18.1.3 from Pseudomonas mendocina (see paper)
28% identity, 95% coverage: 12:325/329 of query aligns to 13:325/326 of Q03304
4wqmA Structure of the toluene 4-monooxygenase nadh oxidoreductase t4mof, k270s k271s variant (see paper)
27% identity, 95% coverage: 12:325/329 of query aligns to 13:325/326 of 4wqmA
- binding flavin-adenine dinucleotide: C36 (= C35), S38 (≠ T37), W57 (≠ Q56), Y135 (= Y134), R146 (= R145), A147 (≠ S146), Y148 (= Y147), S149 (= S148), I162 (≠ H162), V163 (≠ I163), G169 (= G169), K170 (≠ V170), V171 (≠ M171), S172 (= S172), S209 (≠ T211), F325 (= F325)
- binding fe2/s2 (inorganic) cluster: Y34 (= Y33), C36 (= C35), N37 (≠ R36), G39 (= G38), C41 (= C40), G42 (= G41), C44 (= C43), C76 (= C74)
1tvcA Fad and nadh binding domain of methane monooxygenase reductase from methylococcus capsulatus (bath) (see paper)
30% identity, 67% coverage: 107:328/329 of query aligns to 18:247/250 of 1tvcA
- active site: Y63 (= Y147), S64 (= S148), L215 (≠ A296)
- binding dihydroflavine-adenine dinucleotide: N24 (≠ D113), F49 (≠ Y134), R61 (= R145), S62 (= S146), Y63 (= Y147), S64 (= S148), L78 (≠ H162), I79 (= I163), R80 (= R164), L82 (≠ V166), F87 (≠ M171), G128 (= G212), N155 (≠ R242), E159 (≠ D246), S186 (≠ G268), G187 (≠ K269), E242 (≠ D323), F244 (= F325), L245 (≠ V326)
7c3bB Ferredoxin reductase in carbazole 1,9a-dioxygenase (fad apo form) (see paper)
30% identity, 94% coverage: 16:325/329 of query aligns to 20:305/306 of 7c3bB
1krhA X-ray structure of benzoate dioxygenase reductase (see paper)
27% identity, 95% coverage: 17:328/329 of query aligns to 22:337/337 of 1krhA
- active site: C306 (= C297)
- binding flavin-adenine dinucleotide: Y143 (= Y134), R155 (= R145), S156 (= S146), Y157 (= Y147), S158 (= S148), V171 (≠ H162), V172 (≠ I163), G178 (= G169), K179 (≠ V170), M180 (= M171), S181 (= S172), T219 (= T211), E332 (≠ D323), F334 (= F325), S335 (≠ V326), A336 (≠ S327), N337 (≠ S328)
- binding fe2/s2 (inorganic) cluster: M38 (≠ Y33), D39 (≠ S34), C40 (= C35), R41 (= R36), G43 (= G38), C45 (= C40), G46 (= G41), C48 (= C43), C82 (= C74)
7romA Crystal structure of saccharomyces cerevisiae nadh-cytochrome b5 reductase 1 (cbr1) fragment (residues 28-284) bound to fad
27% identity, 59% coverage: 132:326/329 of query aligns to 45:254/255 of 7romA
- binding flavin-adenine dinucleotide: R61 (= R145), S62 (= S146), Y63 (= Y147), T64 (≠ S148), L79 (≠ H162), V80 (≠ I163), Y83 (≠ V166), T85 (≠ D168), G86 (= G169), N87 (≠ V170), V88 (≠ M171), S89 (= S172), T127 (= T211), A130 (= A214)
Q9ZNT1 NADH--cytochrome b5 reductase 1; EC 1.6.2.2 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
25% identity, 61% coverage: 103:302/329 of query aligns to 51:260/281 of Q9ZNT1
- L78 (= L130) mutation to F: In cbr1-1; decreased stability and decreased activity.
Sites not aligning to the query:
- 34:40 AKR2A-binding sequence (ABS) required for mitochondrion outer membrane targeting
2r6hC Crystal structure of the domain comprising the NAD binding and the fad binding regions of the nadh:ubiquinone oxidoreductase, na translocating, f subunit from porphyromonas gingivalis
24% identity, 55% coverage: 145:325/329 of query aligns to 86:288/289 of 2r6hC
- active site: C260 (= C297)
- binding flavin-adenine dinucleotide: R86 (= R145), A87 (≠ S146), Y88 (= Y147), S89 (= S148), N103 (≠ H162), A107 (vs. gap), T108 (vs. gap), I122 (≠ V166), K123 (≠ P167), P124 (≠ D168), G125 (= G169), I126 (≠ V170), S127 (≠ M171), S128 (= S172), F288 (= F325)
Sites not aligning to the query:
6l2uA Soluble methane monooxygenase reductase fad-binding domain from methylosinus sporium. (see paper)
40% identity, 26% coverage: 108:194/329 of query aligns to 12:103/103 of 6l2uA
- binding flavin-adenine dinucleotide: F43 (≠ Y134), R55 (= R145), S56 (= S146), Y57 (= Y147), S58 (= S148), F71 (≠ H162), F71 (≠ H162), I72 (= I163), R73 (= R164), L75 (≠ V166), P76 (= P167), P76 (= P167), G78 (= G169), A79 (≠ V170), F80 (≠ M171), S81 (= S172)
Q68EJ0 Cytochrome b5 reductase 4; Flavohemoprotein b5/b5R; b5+b5R; N-terminal cytochrome b5 and cytochrome b5 oxidoreductase domain-containing protein; cb5/cb5R; EC 1.6.2.2 from Rattus norvegicus (Rat) (see paper)
24% identity, 60% coverage: 102:297/329 of query aligns to 277:490/520 of Q68EJ0
Sites not aligning to the query:
- 89 mutation H->A,M: Abolishes heme-binding but does not affect reductase activity.
- 112 mutation H->A,M: Abolishes heme-binding but does not affect reductase activity.
4g1bA X-ray structure of yeast flavohemoglobin in complex with econazole (see paper)
29% identity, 37% coverage: 101:221/329 of query aligns to 151:292/398 of 4g1bA
- binding flavin-adenine dinucleotide: Y189 (= Y134), R207 (= R144), H208 (≠ R145), Y209 (vs. gap), S210 (vs. gap), A223 (≠ D156), K225 (= K158), E227 (= E160), F233 (≠ V166), P234 (= P167), G236 (= G169), L237 (≠ V170), V238 (≠ M171), S239 (= S172), V282 (≠ T211)
Sites not aligning to the query:
- binding 1-[(2s)-2-[(4-chlorobenzyl)oxy]-2-(2,4-dichlorophenyl)ethyl]-1h-imidazole: 28, 29, 56, 57, 60, 102, 126
- binding flavin-adenine dinucleotide: 46, 50, 84, 390
- binding protoporphyrin ix containing fe: 42, 43, 44, 47, 60, 80, 81, 84, 85, 88, 90, 94, 95, 98, 133, 392, 393, 394
7qu0A X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 53% coverage: 140:315/329 of query aligns to 78:269/279 of 7qu0A
- binding ~{N}-[2,6-bis(fluoranyl)phenyl]ethanamide: S213 (= S265), F227 (≠ Y277), M256 (= M302)
- binding flavin-adenine dinucleotide: R83 (= R145), A84 (≠ S146), Y85 (= Y147), S86 (= S148), A104 (vs. gap), T105 (vs. gap), P106 (vs. gap), P107 (vs. gap), A113 (≠ V166), P114 (= P167), P115 (≠ D168), G116 (= G169), I117 (≠ V170), M118 (= M171), S119 (= S172)
Sites not aligning to the query:
7qtyA X-ray structure of fad domain of nqrf of klebsiella pneumoniae (see paper)
26% identity, 53% coverage: 140:315/329 of query aligns to 78:269/279 of 7qtyA
- binding 1-(furan-2-ylmethyl)-3-(2-methylphenyl)thiourea: A184 (≠ G241), R185 (= R242), S213 (= S265), F227 (≠ Y277), H229 (≠ Q279), V255 (≠ A301), M256 (= M302)
- binding flavin-adenine dinucleotide: R83 (= R145), A84 (≠ S146), Y85 (= Y147), S86 (= S148), N100 (≠ H162), A104 (vs. gap), T105 (vs. gap), P107 (vs. gap), A113 (≠ V166), P114 (= P167), G116 (= G169), I117 (≠ V170), M118 (= M171), S119 (= S172)
Sites not aligning to the query:
7qu5A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
24% identity, 56% coverage: 143:325/329 of query aligns to 81:278/280 of 7qu5A
- binding flavin-adenine dinucleotide: R83 (= R145), A84 (≠ S146), Y85 (= Y147), S86 (= S148), N100 (≠ H162), A104 (vs. gap), L112 (≠ V166), P113 (= P167), G115 (= G169), Q116 (≠ V170), M117 (= M171), S118 (= S172), A155 (≠ T211), F278 (= F325)
- binding gamma-Valerolactone: S105 (vs. gap), P108 (vs. gap), D196 (≠ P254), W207 (≠ Y260), H208 (≠ R261), L209 (≠ P262)
Sites not aligning to the query:
7qu3A X-ray structure of fad domain of nqrf of pseudomonas aeruginosa (see paper)
24% identity, 56% coverage: 143:325/329 of query aligns to 81:278/279 of 7qu3A
- binding flavin-adenine dinucleotide: R83 (= R145), A84 (≠ S146), Y85 (= Y147), S86 (= S148), N100 (≠ H162), A104 (vs. gap), S105 (vs. gap), L112 (≠ V166), P113 (= P167), G115 (= G169), Q116 (≠ V170), M117 (= M171), S118 (= S172), A155 (≠ T211), F278 (= F325)
- binding 4-(benzimidazol-1-ylmethyl)benzenecarbonitrile: R102 (= R164), G154 (= G210), G182 (= G240), F226 (≠ Y277), I227 (≠ V278), P252 (≠ S299), M255 (= M302)
Sites not aligning to the query:
1cnfA Structural studies on corn nitrate reductase: refined structure of the cytochrome b reductase fragment at 2.5 angstroms, its adp complex and an active site mutant and modeling of the cytochrome b domain (see paper)
23% identity, 62% coverage: 102:306/329 of query aligns to 5:241/260 of 1cnfA
- active site: H38 (≠ Y134), Y54 (= Y147), T55 (≠ S148), C232 (= C297)
- binding adenosine-5'-diphosphate: K71 (≠ R164), N165 (≠ G241), P234 (≠ S299), P236 (≠ A301), M237 (= M302), F240 (≠ T305), A241 (= A306)
- binding flavin-adenine dinucleotide: R52 (= R145), A53 (≠ S146), Y54 (= Y147), L69 (≠ H162), Y73 (vs. gap), F81 (≠ V166), G84 (= G169), G85 (≠ V170), L86 (≠ M171), M87 (≠ S172), T88 (≠ Q173), S136 (≠ T211), T139 (≠ A214), P140 (= P215)
Query Sequence
>GFF1621 FitnessBrowser__WCS417:GFF1621
MSIVTLSNHKSFTGESGKSLLECAKSQGTVLEYSCRTGRCGVCKANVLQGDTEVMQPELA
LTDEERANGAILTCCRAALTDVELDIEDLGLLADIQIKTMPCRLDTLERLADDVVRVVLR
TPPKSALFYLPGQYIDVIGKDGVRRSYSIANAVREDAKLELHIRQVPDGVMSQYWFGEAQ
VNDLLRLEGPLGTFCLRNASTTNLVFMATGTGIAPVKAMLEALASTSEPLIGKRVYVYWG
GRTENDLYWLPDFPTLGMTYRPVLSRAGKHWPGATGYVQQAVLAENLDLADTSVYACGSE
AMIHTAREMLTQAGLPLKHFHSDAFVSSN
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory