SitesBLAST

S202 (≠ A252) mutation to C: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, V-332 and V-342.
I332 (≠ W387) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-342.
M342 (≠ V397) mutation to V: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, R-81, C-202 and V-332.

Sites not aligning to the query:

51 G→S: Shows 4.3- and 107-fold increase of affinity to glyphosate and glycine, respectively. Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with R-54, R-81, C-202, V-332 and V-342.
54 A→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-81, C-202, V-332 and V-342.
81 K→R: Shows 7.1- and 8-fold increase of affinity and catalytic efficiency to glyphosate, respectively, while the substrate affinity to glycine decreases 235-fold and catalytic efficiency decreases 113-fold; when associated with S-51, R-54, C-202, V-332 and V-342.

7cyxA Crystal strcuture of glycine oxidase from bacillus cereus atcc 14579 (see paper)
23% identity, 99% coverage: 4:403/406 of query aligns to 4:346/363 of 7cyxA

query
sites
7cyxA

I

V

A

A

V

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I
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I

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G

G

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G

I
x
V

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I

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M

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E

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x
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S

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K

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L

L

L

S

G

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Y

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N

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G

binding flavin-adenine dinucleotide: I7 (= I7), G8 (= G8), G10 (= G10), V11 (≠ I11), I12 (≠ T12), V30 (≠ Y30), E31 (= E31), K32 (= K32), E38 (= E39), A39 (≠ T40), S40 (= S41), A43 (≠ N44), G45 (= G46), L46 (≠ Q47), V171 (= V220), G200 (≠ A252), G201 (= G253), W203 (≠ G255), G298 (= G356), R300 (= R358), P301 (= P359), Y326 (≠ G383), R327 (≠ H384), N328 (≠ L385), G329 (= G386), I330 (≠ W387)

3if9A Crystal structure of glycine oxidase g51s/a54r/h244a mutant in complex with inhibitor glycolate (see paper)
25% identity, 58% coverage: 167:401/406 of query aligns to 121:346/364 of 3if9A

query
sites
3if9A

T

S

P

K

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A

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G

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R

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M

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G

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G

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x
I

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I

binding flavin-adenine dinucleotide: P173 (≠ T219), V174 (= V220), S202 (≠ A252), G203 (= G253), W205 (≠ G255), F209 (≠ L259), G300 (= G356), R302 (= R358), H327 (= H382), F328 (≠ G383), R329 (≠ H384), N330 (≠ L385), G331 (= G386), I332 (≠ W387)
binding glycolic acid: Y246 (≠ K301), R302 (= R358), R329 (≠ H384)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 34, 35, 42, 43, 46, 47, 48, 49

O31616 Glycine oxidase; GO; EC 1.4.3.19 from Bacillus subtilis (strain 168) (see 3 papers)
24% identity, 58% coverage: 167:401/406 of query aligns to 121:346/369 of O31616

query
sites
O31616

T

S

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x
H

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V174 (= V220) binding
H244 (≠ A299) mutation to A: 2-fold decrease in catalytic efficiency on glycine and similar catalytic efficiency on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and R-54.
R302 (= R358) binding
327:333 (vs. 382:388, 29% identical) binding
R329 (≠ H384) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding
51 G→R: 130-fold decrease in catalytic efficiency on glycine and 28-fold increase in that on glyphosate.; G→S: 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with R-54 and A-244.
54 A→R: 20-fold decrease in catalytic efficiency on glycine and 34-fold increase in that on glyphosate. 60-fold decrease in catalytic efficiency on glycine and 210-fold increase in that on glyphosate; when associated with S-51 and A-244.

1ng3A Complex of thio (glycine oxidase) with acetyl-glycine (see paper)
24% identity, 58% coverage: 167:401/406 of query aligns to 121:346/364 of 1ng3A

query
sites
1ng3A

T

S

P

K

E

E

M

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K

L

S

E

I

K

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E

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P

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Y

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x
F

A

F

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N

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K
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Y

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G

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R

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S

A

A

P

I

A

T

T

A

G

E

A

L

L

V

I

A

S

E

D

S

L

V

I

binding acetylamino-acetic acid: Y246 (≠ K301), R302 (= R358), R329 (≠ H384)
binding flavin-adenine dinucleotide: V174 (= V220), S202 (≠ A252), G203 (= G253), W205 (≠ G255), F209 (≠ L259), G300 (= G356), R302 (= R358), H327 (= H382), R329 (≠ H384), N330 (≠ L385), G331 (= G386), I332 (≠ W387)
binding phosphate ion: R254 (= R311)

Sites not aligning to the query:

active site: 47, 48, 49
binding flavin-adenine dinucleotide: 11, 13, 15, 33, 34, 35, 41, 42, 43, 46, 47, 48, 49
binding phosphate ion: 89

4x9mA Oxidized l-alpha-glycerophosphate oxidase from mycoplasma pneumoniae with fad bound (see paper)
49% identity, 11% coverage: 2:46/406 of query aligns to 4:48/384 of 4x9mA

query
sites
4x9mA

K

R

R

D

I

V

A

L

V

I

I

V

G
|
G

G

G

G
|
G

I

V

T
x
I

G

G

I

C

T

A

T

T

A

A

Y

Y

T

E

L

L

A

S

K

Q

R

Y

G

K

L

L

D

K

V

V

T

T

V

L

Y

V

E
|
E

K
|
K

H

H

R

H

Y

Y

A

L

A

A

M

Q

E
|
E

T
|
T

S
|
S

F

H

A
|
A

N
|
N

G
x
S

G

G

binding flavin-adenine dinucleotide: G10 (= G8), G12 (= G10), I14 (≠ T12), E33 (= E31), K34 (= K32), E41 (= E39), T42 (= T40), S43 (= S41), A45 (= A43), N46 (= N44), S47 (≠ G45)
binding sn-glycerol-3-phosphate: S47 (≠ G45)

Sites not aligning to the query:

binding flavin-adenine dinucleotide: 49, 51, 176, 177, 209, 210, 212, 234, 319, 320, 346, 347, 348, 349, 350, 351, 352
binding sn-glycerol-3-phosphate: 51, 258, 259, 320, 348

P75063 Glycerol 3-phosphate oxidase; GlpO; L-alpha-glycerophosphate oxidase; EC 1.1.3.21 from Mycoplasma pneumoniae (strain ATCC 29342 / M129 / Subtype 1) (Mycoplasmoides pneumoniae) (see paper)
49% identity, 11% coverage: 2:46/406 of query aligns to 4:48/384 of P75063

query
sites
P75063

K

R

R

D

I

V

A

L

V

I

I

V

G

G

I

V

T
x
I

G

G

I

C

T

A

T

T

A

A

Y

Y

T

E

L

L

A

S

K

Q

R

Y

G

K

L

L

D

K

V

V

T

T

V

L

Y

V

E
|
E

K

K

H

H

R

H

Y

Y

A

L

A

A

M

Q

E

E

T
|
T

S
|
S

F

H

A

A

N

N

G
x
S

G
|
G

I14 (≠ T12) binding
E33 (= E31) binding
TS 42:43 (= TS 40:41) binding

Sites not aligning to the query:

47:49 binding
177 binding
346:347 binding
352 binding

4yshA Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 72% coverage: 110:401/406 of query aligns to 64:351/370 of 4yshA

query
sites
4yshA

L

L

A

A

I

L

A

Q

A

S

R

R

D

A

H

L

L

M

F

P

A

A

W

L

A

A

Q

E

E

E

-

L

-

R

-

E

-

R

E

T

G

G

I

I

D

D

F

I

D

G

L

L

K

V

K

E

Q

K

G

G

I

L

L

I

H

K

I

L

Y

A

R

T

D

T

K

E

A

E

G

E

F

A

D

D

H

D

A

L

E

Y

K

R

V

H

S

Y

R

T

L

F

L

W

A

R

A

G

G

I

G

G

L

E

E

P

R

V

R

Q

S

W

V

L

T

T

P

K

E

G

E

E

M

A

K

L

S

E

I

M

E

E

P

P

T

R

L

L

A

A

G

E

N

A

Y

L

Y

A

G

G

G

A

F

M

F

Y

T

I

E

P

S

G

D

D

S

-

T

-

G

G

D

Q

I

V

H

S

K

A

F

P

T

D

N

L

G

A

L

A

A

A

D

L

A

A

I

Y

K

A

R

A

L

A

G

S

V

A

K

G

T

A

C

C

-

L

Y

Y

G

E

H

Y

T

T

-

E

V
|
V

T

F

E

D

L

I

S

R

A

S

D

D

E

S

R

S

N

G

A

H

W

V

V

L

T

D

A

T

H

T

D

G

G

G

T

T

E

F

Q

A

S

A

R

-

D

-

T

-

F

-

D

E

G

A

V

V

I

I

C

A

A
x
S

G
|
G

V

A

G
x
W

S

A

R

A

G

R

L

L

A

G

K

A

K

R

L

V

G

G

D

L

R

S

V

L

N

S

I

V

Y

Y

P

P

V

V

K

K

G

G

Y

E

S

C

I

V

T

M

V

V

E

-

L

-

D

-

E

R

A

A

S

P

Q

V

K

P

A

L

P

Q

T

T

V

T

S

V

L

F

L

A

D

K

D

N

A

G

T

C

K

Y

I

I

V

V

T

P

S

K

R

-

L

-

G

S

D

G

G

N

R

R

F

L

R

L

V
x
I

A

G

G

A

T

T

A

S

E

T

F

P

S

G

G

T

Y

F

N

D

R

R

D

R

I

V

K

S

D

A

D

G

R

G

I

V

R

M

P

N

L
|
L

T

L

R

H

W

R

V

A

E

A

Q

H

C

L

F

V

P

P

G

D

V

I

C

E

T

Q

R

A

K

E

-

W

V

V

V

A

P

S

W

W

A

S

G
|
G

L

I

R
|
R

P

P

M

Q

L

T

P

E

N

D

M

G

M

L

P

P

R

Y

V

L

G

G

-

E

-

H

P

P

G

E

R

R

L

R

P

-

T

G

V

L

F

F

Y

V

N

A

T

A

G

G

H
|
H

G

Y

H
x
R

L
x
N

G
|
G

W
x
I

T
x
L

L

L

S

S

A

P

I

L

T

T

A

G

E

L

L

L

V

V

A

A

E

D

S

L

V

V

active site: I262 (≠ V314), L283 (= L335), G305 (= G356), N335 (≠ L385), L338 (≠ T388)
binding flavin-adenine dinucleotide: V178 (= V220), S206 (≠ A252), G207 (= G253), W209 (≠ G255), R307 (= R358), H332 (= H382), R334 (≠ H384), N335 (≠ L385), G336 (= G386), I337 (≠ W387)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 47, 48

4yshB Crystal structure of glycine oxidase from geobacillus kaustophilus
24% identity, 72% coverage: 110:401/406 of query aligns to 64:351/368 of 4yshB

query
sites
4yshB

L

L

A

A

I

L

A

Q

A

S

R

R

D

A

H

L

L

M

F

P

A

A

W

L

A

A

Q

E

E

E

-

L

-

R

-

E

-

R

E

T

G

G

I

I

D

D

F

I

D

G

L

L

K

V

K

E

Q

K

G

G

I

L

L

I

H

K

I

L

Y

A

R

T

D

T

K

E

A

E

G

E

F

A

D

D

H

D

A

L

E

Y

K

R

V

H

S

Y

R

T

L

F

L

W

A

R

A

G

G

I

G

G

L

E

E

P

R

V

R

Q

S

W

V

L

T

T

P

K

E

G

E

E

M

A

K

L

S

E

I

M

E

E

P

P

T

R

L

L

A

A

G

E

N

A

Y

L

Y

A

G

G

G

A

F

M

F

Y

T

I

E

P

S

G

D

D

S

-

T

-

G

G

D

Q

I

V

H

S

K

A

F

P

T

D

N

L

G

A

L

A

A

A

D

L

A

A

I

Y

K

A

R

A

L

A

G

S

V

A

K

G

T

A

C

C

-

L

Y

Y

G

E

H

Y

T

T

-

E

V
|
V

T

F

E

D

L

I

S

R

A

S

D

D

E

S

R

S

N

G

A

H

W

V

V

L

T

D

A

T

H

T

D

G

G

G

T

T

E

F

Q

A

S

A

R

-

D

-

T

-

F

-

D

E

G

A

V

V

I

I

C

A

A
x
S

G

G

V

A

G
x
W

S

A

R

A

G

R

L

L

A

G

K

A

K

R

L

V

G

G

D

L

R

S

V

L

N

S

I

V

Y

Y

P

P

V

V

K

K

G

G

Y

E

S

C

I

V

T

M

V

V

E

-

L

-

D

-

E

R

A

A

S

P

Q

V

K

P

A

L

P

Q

T

T

V

T

S

V

L

F

L

A

D

K

D

N

A
x
G

T

C

K
x
Y

I

I

V

V

T

P

S

K

R

-

L

-

G

S

D

G

G

N

R

R

F

L

R

L

V
x
I

A

G

G
x
A

T

T

A

S

E

T

F

P

S

G

G

T

Y

F

N

D

R

R

D

R

I

V

K

S

D

A

D

G

R

G

I

V

R

M

P

N

L
|
L

T

L

R

H

W

R

V

A

E

A

Q

H

C

L

F

V

P

P

G

D

V

I

C

E

T

Q

R

A

K

E

-

W

V

V

V

A

P

S

W

W

A

S

G
|
G

L

I

R
|
R

P

P

M

Q

L

T

P

E

N

D

M

G

M

L

P

P

R

Y

V

L

G

G

-

E

-

H

P

P

G

E

R

R

L

R

P

-

T

G

V

L

F

F

Y

V

N

A

T

A

G

G

H
|
H

G

Y

H
x
R

L
x
N

G
|
G

W
x
I

T
x
L

L

L

S

S

A

P

I

L

T

T

A

G

E

L

L

L

V

V

A

A

E

D

S

L

V

V

active site: I262 (≠ V314), L283 (= L335), G305 (= G356), N335 (≠ L385), L338 (≠ T388)
binding flavin-adenine dinucleotide: V178 (= V220), S206 (≠ A252), W209 (≠ G255), R307 (= R358), H332 (= H382), R334 (≠ H384), N335 (≠ L385), G336 (= G386), I337 (≠ W387), L338 (≠ T388)
binding glycine: G249 (≠ A299), Y251 (≠ K301), Y251 (≠ K301), A264 (≠ G316), R307 (= R358), R334 (≠ H384), R334 (≠ H384)

Sites not aligning to the query:

active site: 45, 48, 49, 52
binding flavin-adenine dinucleotide: 9, 10, 12, 14, 32, 33, 34, 40, 41, 42, 45, 46, 48

Q5L2C2 Glycine oxidase; GO; GOX; GOXK; EC 1.4.3.19 from Geobacillus kaustophilus (strain HTA426)
24% identity, 72% coverage: 110:401/406 of query aligns to 65:353/377 of Q5L2C2

query
sites
Q5L2C2

L

L

A

A

I

L

A

Q

A

S

R

R

D

A

H

L

L

M

F

P

A

A

W

L

A

A

Q

E

E

E

-

L

-

R

-

E

-

R

E

T

G

G

I

I

D

D

F

I

D

G

L

L

K

V

K

E

Q

K

G

G

I

L

L

I

H

K

I

L

Y

A

R

T

D

T

K

E

A

E

G

E

F

A

D

D

H

D

A

L

E

Y

K

R

V

H

S

Y

R

T

L

F

L

W

A

R

A

G

G

I

G

G

L

E

E

P

R

V

R

Q

S

W

V

L

T

T

P

K

E

G

E

E

M

A

K

L

S

E

I

M

E

E

P

P

T

R

L

L

A

A

G

A

N

E

Y

A

Y

L

G

A

G

G

-

A

F

M

F

Y

T

I

E

P

S

G

D

D

S

-

T

-

G

G

D

Q

I

V

H

S

K

A

F

P

T

D

N

L

G

A

L

A

A

A

D

L

A

A

I

Y

K

A

R

A

L

A

G

S

V

A

K

G

T

A

C

C

-

L

Y

Y

G

E

H

Y

T

T

-

E

V
|
V

T

F

E

D

L

I

S

R

A

S

D

D

E

S

R

S

N

G

A

H

W

V

V

L

T

D

A

T

H

T

D

G

G

G

T

T

E

F

Q

A

S

A

R

-

D

-

T

-

F

-

D

E

G

A

V

V

I

I

C

A

A

S

G

G

V

A

G

W

S

A

R

A

G

R

L

L

A

G

K

A

K

R

L

V

G

G

D

L

R

S

V

L

N

S

I

V

Y

Y

P

P

V

V

K

K

G

G

Y

E

S

C

I

V

T

M

V

V

E

-

L

-

D

-

E

R

A

A

S

P

Q

V

K

P

A

L

P

Q

T

T

V

T

S

V

L

F

L

A

D

K

D

N

A

G

T

C

K

Y

I

I

V

V

T

P

S

K

R

-

L

-

G

S

D

G

G

N

R

R

F

L

R

L

V

I

A

G

G

A

T

T

A

S

E

T

F

P

S

G

G

T

Y

F

N

D

R

R

D

R

I

V

K

S

D

A

D

G

R

G

I

V

R

M

P

N

L

L

T

L

R

H

W

R

V

A

E

A

Q

H

C

L

F

V

P

P

G

D

V

I

C

E

T

Q

R

A

K

E

-

W

V

V

V

A

P

S

W

W

A

S

G

G

L

I

R
|
R

P

P

M

Q

L

T

P

E

N

D

M

G

M

L

P

P

R

Y

V

L

G

G

-

E

-

H

P

P

G

E

R

R

L

R

P

-

T

G

V

L

F

F

Y

V

N

A

T

A

G

G

H
|
H

G
x
Y

H
x
R

L
x
N

G
|
G

W
x
I

T
x
L

L

L

S

S

A

P

I

L

T

T

A

G

E

L

L

L

V

V

A

A

E

D

S

L

V

V

V180 (= V220) binding
R309 (= R358) binding
334:340 (vs. 382:388, 29% identical) binding
R336 (≠ H384) binding

Sites not aligning to the query:

14:15 binding
34:35 binding
42:43 binding
47:49 binding

Query Sequence

>GFF1637 FitnessBrowser__Marino:GFF1637
MKRIAVIGGGITGITTAYTLAKRGLDVTVYEKHRYAAMETSFANGGQLSASNAEVWNNWQ
TVMKGIKWMSRRDAPLLVNPKPSWHKLSWFAEFIAAIPQYEKNTTETARLAIAARDHLFA
WAQEEGIDFDLKKQGILHIYRDKAGFDHAEKVSRLLAAGGLERRSVTPEEMKSIEPTLAG
NYYGGFFTESDSTGDIHKFTNGLADAIKRLGVKTCYGHTVTELSADERNAWVTAHDGTEQ
SRDTFDGVVICAGVGSRGLAKKLGDRVNIYPVKGYSITVELDDEASQKAAPTVSLLDDAT
KIVTSRLGDGRFRVAGTAEFSGYNRDIKDDRIRPLTRWVEQCFPGVCTRKVVPWAGLRPM
LPNMMPRVGPGRLPTVFYNTGHGHLGWTLSAITAELVAESVTGDSR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory