SitesBLAST

8vr2B Crystal structure of the pcryo_0617 oxidoreductase/decarboxylase from psychrobacter cryohalolentis k5 in the presence of NAD and udp
25% identity, 98% coverage: 3:308/313 of query aligns to 10:307/321 of 8vr2B

query
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8vr2B

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binding nicotinamide-adenine-dinucleotide: G14 (= G7), G17 (= G10), F18 (= F11), I19 (≠ L12), D37 (= D24), N38 (≠ R25), E40 (≠ T27), R41 (≠ L28), N61 (≠ D48), V62 (≠ L49), A81 (= A68), A82 (= A69), A83 (= A70), F124 (≠ I112), K154 (= K144), P177 (= P167), N179 (≠ L169)
binding uridine-5'-diphosphate: R147 (vs. gap), G189 (≠ F179), A190 (= A180), M194 (vs. gap), Y205 (≠ L192), I206 (≠ P193), F207 (= F194), R214 (= R201), I251 (≠ L241)

6bwlA X-ray structure of pal from bacillus thuringiensis (see paper)
25% identity, 100% coverage: 1:313/313 of query aligns to 1:310/313 of 6bwlA

query
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6bwlA

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active site: T122 (≠ S114), C123 (≠ I115), M124 (≠ K116), Y147 (= Y140), K151 (= K144)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (≠ S29), N32 (≠ C30), L33 (≠ A31), N35 (≠ R33), S36 (= S34), D57 (= D54), I58 (≠ W55), L79 (≠ A68), A80 (= A69), A81 (= A70), I83 (≠ A72), M120 (≠ I112), K151 (= K144), N176 (≠ L169), T177 (≠ V170)
binding uridine-5'-diphosphate: N176 (≠ L169), G189 (= G177), V190 (≠ N178), N205 (≠ P193), I206 (vs. gap), Y207 (≠ F194), Q212 (≠ N199), R214 (= R201), I250 (≠ M248), E275 (≠ M274)

4zrnA Crystal structure of udp-glucose 4-epimerase (tm0509) with udp-glucose from hyperthermophilic eubacterium thermotoga maritima (see paper)
24% identity, 99% coverage: 1:310/313 of query aligns to 1:302/309 of 4zrnA

query
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4zrnA

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active site: T117 (≠ S114), G119 (vs. gap), A120 (≠ K116), Y143 (= Y140), K147 (= K144), Y181 (vs. gap), G185 (= G177)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (≠ S34), N32 (≠ P35), S34 (≠ A37), S35 (≠ Q38), G36 (≠ V39), S51 (≠ D54), I52 (≠ W55), L73 (≠ A68), A74 (= A69), A75 (= A70), T92 (≠ V89), S115 (≠ I112), S116 (= S113), Y143 (= Y140), K147 (= K144), Y170 (≠ P167), V173 (= V170)
binding uridine-5'-diphosphate-glucose: T117 (≠ S114), G119 (vs. gap), A120 (≠ K116), Y143 (= Y140), N172 (≠ L169), G185 (= G177), V186 (≠ N178), H201 (≠ P193), F203 (= F194), Y208 (≠ N199), R210 (= R201), V244 (≠ T237), R267 (≠ K271), D270 (≠ M274)

4id9A Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
37% identity, 52% coverage: 3:166/313 of query aligns to 2:158/321 of 4id9A

query
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4id9A

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T

V

V

I

I

I

L

R

R

active site: S106 (= S114), Y132 (= Y140), K136 (= K144)
binding nicotinamide-adenine-dinucleotide: G9 (= G10), R10 (≠ F11), V11 (≠ L12), D30 (≠ A31), L31 (≠ V32), R32 (= R33), S44 (≠ D48), L45 (= L49), L64 (≠ A68), A66 (= A70), V81 (= V89), S106 (= S114), Y132 (= Y140), K136 (= K144)

Sites not aligning to the query:

4id9B Crystal structure of a short-chain dehydrogenase/reductase superfamily protein from agrobacterium tumefaciens (target efi-506441) with bound NAD, monoclinic form 1
37% identity, 52% coverage: 3:166/313 of query aligns to 3:159/328 of 4id9B

query
sites
4id9B

V

I

L

L

L

V

T

T

G

G

A

S

N

A

G
|
G

F
x
R

L
x
V

G

G

R

R

A

A

I

V

V

V

A

A

H

A

L

L

C

R

R

T

Q

Q

D

G

R

R

I

T

T

V

L

R

S

G

C

F

A
x
D

V
x
L

R

R

S

P

P

S

L

G

A

T

Q

G

V

-

R

-

F

-

A

G

T

E

F

E

A

V

V

V

G

G

D
x
S

L
|
L

C

E

G

D

A

G

N

Q

D

A

W

L

S

S

Q

D

P

A

L

I

L

M

G

G

Q

V

Q

S

V

A

V

V

I

L

H

H

A
x
L

A

G

A
|
A

R

-

A

-

H

-

I

F

M

M

K

S

D

W

E

A

L

P

A

A

D

D

P

R

L

D

S

R

E

M

Y

F

R

-

L

A

V
|
V

N

N

V

V

E

E

G

G

T

T

L

R

N

R

L

L

A

L

R

D

Q

A

A

A

A

S

A

A

G

G

V

V

E

R

R

R

F

F

I

V

Y

F

I

A

S

S

S
|
S

I

G

K

E

V

V

N

Y

G

P

E

E

S

N

T

R

P

P

L

E

G

F

K

L

P

P

F

-

V

V

S

T

S

E

D

D

A

H

P

P

-

L

A

C

P

P

E

N

D

S

P

P

Y
|
Y

G

G

L

L

S

T

K
|
K

L

L

E

L

A

G

E

E

Q

E

G

-

L

L

M

V

Q

R

L

F

A

H

A

Q

E

R

T

S

G

G

-

A

M

M

E

E

V

T

V

V

I

I

I

L

R

R

active site: S107 (= S114), Y133 (= Y140), K137 (= K144)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), R11 (≠ F11), V12 (≠ L12), D31 (≠ A31), L32 (≠ V32), S45 (≠ D48), L46 (= L49), L65 (≠ A68), A67 (= A70), V82 (= V89), Y133 (= Y140), K137 (= K144)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 160, 161, 162, 163

2ydxE Crystal structure of human s-adenosylmethionine synthetase 2, beta subunit (see paper)
29% identity, 59% coverage: 3:188/313 of query aligns to 5:177/312 of 2ydxE

query
sites
2ydxE

V

V

L

L

L

V

T

T

G
|
G

A

A

N
x
T

G
|
G

F
x
L

L
|
L

G

G

R

R

A

A

I

V

V

H

A

K

H

E

L

F

C

Q

R

Q

Q

N

D

N

R

W

I

H

T

A

L

V

S

G

C

C

A

G

V
x
F

R
|
R

S
x
R

P

-

L

-

A

A

Q

R

V

P

R

K

F

F

A
x
E

T

Q

F
x
V

A
x
N

V
x
L

G

L

D

D

L

S

C

N

G
x
A

A

V

N

H

D
x
H

W

I

S

I

Q

H

P
x
D

L

F

L

Q

G

P

Q

H

Q

V

V

I

V

V

I

H

H
x
C

A
|
A

A

E

R
|
R

A
x
R

H

P

I

D

M

V

K

V

D

E

E

N

L

Q

A

P

D

D

P

A

L

A

S

S

E

Q

Y

-

R

-

L

-

V
x
L

N

N

V

V

E

D

G

A

T

S

L

G

N

N

L

L

A

A

R

K

Q

E

A

A

A

V

G

G

V

A

E

-

R

F

F

L

I

I

Y

Y

I
|
I

S

S

S
|
S

I
x
D

K

Y

V

V

N

F

G

D

E

G

S

T

T

N

P

P

L

-

G

-

K

-

P

P

F

Y

V

R

S

E

D

D

A

I

P

P

A

A

P

P

E

L

D

N

P

L

Y
|
Y

G

G

L

K

S

T

K
|
K

L

L

E

D

A

G

E

E

Q

K

G

A

L

V

M

L

Q

-

L

-

A

-

E

E

T

N

G

N

M

L

E

G

V

A

I

V

I

L

R

R

P
x
I

P
|
P

L
x
I

V
x
L

Y

Y

G

G

P

E

G

V

V

E

K

K

G

L

N

E

F

E

A

S

S
x
A

M

V

I

T

K

V

L

M

I

F

D

D

R

K

binding resveratrol: E42 (≠ A42), A51 (≠ G51), H54 (≠ D54), D58 (≠ P58), R71 (= R71), R72 (≠ A72), S110 (= S114), D111 (≠ I115), Y133 (= Y140), I158 (≠ L169), A170 (≠ S181)
binding 1,4,5,6-tetrahydronicotinamide adenine dinucleotide phosphate: G9 (= G7), T11 (≠ N9), G12 (= G10), L13 (≠ F11), L14 (= L12), F34 (≠ V32), R35 (= R33), R36 (≠ S34), V44 (≠ F44), N45 (≠ A45), L46 (≠ V46), C67 (≠ H67), A68 (= A68), A69 (= A69), R71 (= R71), L86 (≠ V89), I108 (= I112), Y133 (= Y140), K137 (= K144), I156 (≠ P167), P157 (= P168), L159 (≠ V170)

Sites not aligning to the query:

binding resveratrol: 193, 303, 305, 306, 308, 310, 311

2ydxA Crystal structure of human s-adenosylmethionine synthetase 2, beta subunit (see paper)
29% identity, 59% coverage: 3:188/313 of query aligns to 5:177/312 of 2ydxA

query
sites
2ydxA

V

V

L

L

L

V

T

T

G
|
G

A

A

N
x
T

G
|
G

F
x
L

L
|
L

G

G

R

R

A

A

I

V

V

H

A

K

H

E

L

F

C

Q

R

Q

Q

N

D

N

R

W

I

H

T

A

L

V

S

G

C

C

A

G

V
x
F

R
|
R

S
x
R

P

-

L

-

A

A

Q

R

V

P

R

K

F

F

A
x
E

T

Q

F
x
V

A

N

V
x
L

G

L

D

D

L

S

C
x
N

G
x
A

A

V

N

H

D
x
H

W

I

S

I

Q

H

P
x
D

L

F

L

Q

G

P

Q

H

Q

V

V

I

V

V

I

H

H
x
C

A

A

A
|
A

A

E

R
|
R

A

R

H

P

I

D

M

V

K

V

D

E

E

N

L

Q

A

P

D

D

P

A

L

A

S

S

E

Q

Y

-

R

-

L

-

V
x
L

N

N

V

V

E

D

G

A

T

S

L

G

N

N

L

L

A

A

R

K

Q

E

A

A

A

V

G

G

V

A

E

-

R

F

F

L

I

I

Y

Y

I
|
I

S

S

S
|
S

I
x
D

K
x
Y

V

V

N

F

G

D

E

G

S

T

T

N

P

P

L

-

G

-

K

-

P

P

F

Y

V

R

S

E

D

D

A

I

P

P

A

A

P

P

E

L

D

N

P

L

Y
|
Y

G

G

L

K

S

T

K
|
K

L

L

E

D

A

G

E

E

Q

K

G

A

L

V

M

L

Q

-

L

-

A

-

E

E

T

N

G

N

M

L

E

G

V

A

I

V

I

L

R

R

P
x
I

P
|
P

L
x
I

V
x
L

Y

Y

G

G

P

E

G

V

V

E

K

K

G

L

N
x
E

F

E

A

S

S
x
A

M

V

I

T

K

V

L

M

I

F

D

D

R

K

binding calcium ion: D111 (≠ I115)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), T11 (≠ N9), G12 (= G10), L13 (≠ F11), L14 (= L12), F34 (≠ V32), R35 (= R33), R36 (≠ S34), V44 (≠ F44), L46 (≠ V46), C67 (≠ H67), A69 (= A69), R71 (= R71), L86 (≠ V89), I108 (= I112), Y133 (= Y140), K137 (= K144), I156 (≠ P167), P157 (= P168), I158 (≠ L169), L159 (≠ V170)
binding resveratrol: E42 (≠ A42), N50 (≠ C50), A51 (≠ G51), H54 (≠ D54), D58 (≠ P58), R71 (= R71), S110 (= S114), D111 (≠ I115), Y112 (≠ K116), Y133 (= Y140), I158 (≠ L169), E167 (≠ N178), A170 (≠ S181)

Sites not aligning to the query:

binding calcium ion: 194, 267
binding resveratrol: 193, 303, 305, 306, 308, 310, 311

1kewA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with thymidine diphosphate bound (see paper)
22% identity, 99% coverage: 1:310/313 of query aligns to 1:332/361 of 1kewA

query
sites
1kewA

M

M

N

K

V

I

L

L

L

I

T

T

G

G

A

G

N

A

G
|
G

F
|
F

L
x
I

G

G

R

S

A

A

I

V

V

V

A

R

H

H

L

I

C

I

R

K

Q

N

-

T

-

Q

-

D

-

T

-

V

-

N

-

I

D
|
D

R
x
K

I
x
L

T
|
T

L

Y

S
x
A

C
x
G

A

N

V

L

R

E

-

S

-

L

S

S

P

D

L

I

A

S

Q

E

V

S

R

N

F

R

A

Y

T

N

F

F

A

E

V

H

G

A

D
|
D

L
x
I

C

C

G

D

A

S

N

A

D

E

W

I

S

T

Q

R

P

I

L

F

L

E

G

Q

Q

Y

Q

Q

-

P

-

D

V

A

V

V

I

M

H

H

A
x
L

A
|
A

R

E

A
x
S

H

H

I

V

M

D

K

R

D

-

E

S

L

I

A

T

D

G

P

P

L

A

S

A

-

F

-

I

E

E

Y
x
T

R

N

L

I

V

V

N

G

V

T

E

Y

G

A

T

L

L

L

N

E

L

V

A

A

R

R

Q

K

A

Y

A

W

A

S

A

A

G

L

V

G

E

E

-

D

-

K

-

N

-

F

R

R

F

F

I

H

Y

H

I
|
I

S
|
S

S
x
T

I
x
D

K
x
E

V

V

N

Y

G

G

E

D

-

L

-

P

-

H

-

P

-

D

-

E

-

V

-

E

-

N

-

S

-

V

S

T

T
x
L

P

P

L
|
L

G

-

K

-

P

-

F
|
F

V

T

S

E

S
x
T

D

T

A

A

P

Y

A

A

P

P

E

S

D

S

P

P

Y
|
Y

G

S

L

A

S

S

K
|
K

L

A

E

S

A

S

E

D

Q

H

G

L

L

V

M

R

Q

A

L

W

A

R

E

T

T

Y

G

G

M

L

E

P

V

T

V

I

I

V

I

T

R

N

P
x
C

P

S

L
x
N

V
x
N

Y

Y

G

G

P

P

G

-

V

-

K

-

G

-

N

-

F

Y

A

H

S

F

M

P

I

E

K
|
K

L
|
L

I

I

D

P

-

L

-

V

-

I

-

L

-

N

-

A

-

L

R

E

G

G

I

K

P

P

L

L

P
|
P

-

I

F
x
Y

G

G

A

K

I

G

H

D

N

Q

K

I

R
|
R

S

D

L

W

V

L

G

Y

V

V

D

E

N

D

L

H

V

A

D

R

L

A

I

L

I

H

R

M

C

V

V

V

D

T

H

-

P

E

A

G

A

K

A

A

N

G

Q

E

I

T

F

Y

L

N

A

I

G

G

D

G

G

H

K

N

D

E

L

K

S

K

T
x
N

T

L

E

D

L

V

L

F

G

T

V

I

G

C

K

D

A

L

M

L

D

D

K

-

P

-

A

-

K

E

L

I

I

V

P

P

A

K

P

A

A

T

G

S

F

Y

L

R

Q

E

L

Q

G

I

A

T

T

Y

L

V

L

A

G

D

K
x
R

K

P

A

G

M
x
H

A

D

Q

R

R

R

L

-

G

-

S

-

L

Y

Q

A

V

I

D

D

I

A

S

G

K

K

T

I

C

S

E

R

L

E

L

L

D

G

W

W

K

K

P

P

P

L

Y

E

T

T

V

F

E

E

E

S

G

G

L

I

R

R

R

K

C

T

F

V

E

E

active site: T133 (≠ S114), D134 (≠ I115), E135 (≠ K116), L152 (≠ T122), L154 (= L124), F155 (= F128), T158 (≠ S131), Y167 (= Y140), K171 (= K144)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ L12), D32 (= D24), K33 (≠ R25), L34 (≠ I26), T35 (= T27), A37 (≠ S29), G38 (≠ C30), D58 (= D48), I59 (≠ L49), L80 (≠ A68), A81 (= A69), A82 (= A70), S84 (≠ A72), T99 (≠ Y86), I131 (= I112), S132 (= S113), T133 (≠ S114), Y167 (= Y140), K171 (= K144), C194 (≠ P167), N196 (≠ L169), N197 (≠ V170)
binding thymidine-5'-diphosphate: E135 (≠ K116), N196 (≠ L169), K206 (= K184), L207 (= L185), P222 (= P193), Y224 (≠ F194), R231 (= R201), N266 (≠ T237), R297 (≠ K271), H300 (≠ M274)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 357

1keuA The crystal structure of dtdp-d-glucose 4,6-dehydratase (rmlb) from salmonella enterica serovar typhimurium with dtdp-d-glucose bound (see paper)
22% identity, 99% coverage: 1:310/313 of query aligns to 1:332/361 of 1keuA

query
sites
1keuA

M

M

N

K

V

I

L

L

L

I

T

T

G

G

A

G

N

A

G
|
G

F
|
F

L
x
I

G

G

R

S

A

A

I

V

V

V

A

R

H

H

L

I

C

I

R

K

Q

N

-

T

-

Q

-

D

-

T

-

V

-

N

-

I

D
|
D

R
x
K

I
x
L

T
|
T

L

Y

S

A

C
x
G

A

N

V

L

R

E

-

S

-

L

S

S

P

D

L

I

A

S

Q

E

V

S

R

N

F

R

A

Y

T

N

F

F

A

E

V

H

G

A

D
|
D

L

I

C

C

G

D

A

S

N

A

D

E

W

I

S

T

Q

R

P

I

L

F

L

E

G

Q

Q

Y

Q

Q

-

P

-

D

V

A

V

V

I

M

H

H

A
x
L

A
|
A

R

E

A
x
S

H

H

I

V

M

D

K

R

D

-

E

S

L

I

A

T

D

G

P

P

L

A

S

A

-

F

-

I

E

E

Y
x
T

R

N

L

I

V

V

N

G

V

T

E

Y

G

A

T

L

L

L

N

E

L

V

A

A

R

R

Q

K

A

Y

A

W

A

S

A

A

G

L

V

G

E

E

-

D

-

K

-

N

-

F

R

R

F

F

I

H

Y

H

I

I

S
|
S

S
x
T

I
x
D

K
x
E

V

V

N

Y

G

G

E

D

-

L

-

P

-

H

-

P

-

D

-

E

-

V

-

E

-

N

-

S

-

V

S

T

T
x
L

P

P

L
|
L

G

-

K

-

P

-

F
|
F

V

T

S

E

S
x
T

D

T

A

A

P

Y

A

A

P

P

E

S

D

S

P

P

Y
|
Y

G

S

L

A

S

S

K
|
K

L

A

E

S

A

S

E

D

Q

H

G

L

L

V

M

R

Q

A

L

W

A

R

E

T

T

Y

G

G

M

L

E

P

V

T

V

I

I

V

I

T

R

N

P
x
C

P

S

L
x
N

V
x
N

Y

Y

G

G

P

P

G

-

V

-

K

-

G

-

N

-

F

Y

A

H

S

F

M

P

I

E

K
|
K

L
|
L

I

I

D

P

-

L

-

V

-

I

-

L

-

N

-

A

-

L

R

E

G

G

I

K

P

P

L

L

P
|
P

-

I

F
x
Y

G

G

A

K

I

G

H

D

N

Q

K

I

R
|
R

S

D

L

W

V

L

G

Y

V

V

D

E

N

D

L

H

V

A

D

R

L

A

I

L

I

H

R

M

C

V

V

V

D

T

H

-

P

E

A

G

A

K

A

A

N

G

Q

E

I

T

F

Y

L

N

A

I

G

G

D

G

G

H

K

N

D

E

L

K

S

K

T
x
N

T

L

E

D

L

V

L

F

G

T

V

I

G

C

K

D

A

L

M

L

D

D

K

-

P

-

A

-

K

E

L

I

I

V

P

P

A

K

P

A

A

T

G

S

F

Y

L

R

Q

E

L

Q

G

I

A

T

T

Y

L

V

L

A

G

D

K
x
R

K

P

A

G

M
x
H

A

D

Q

R

R

R

L

-

G

-

S

-

L

Y

Q

A

V

I

D

D

I

A

S

G

K

K

T

I

C

S

E

R

L

E

L

L

D

G

W

W

K

K

P

P

P

L

Y

E

T

T

V

F

E

E

E

S

G

G

L

I

R

R

R

K

C

T

F

V

E

E

active site: T133 (≠ S114), D134 (≠ I115), E135 (≠ K116), L152 (≠ T122), L154 (= L124), F155 (= F128), T158 (≠ S131), Y167 (= Y140), K171 (= K144)
binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: S84 (≠ A72), T133 (≠ S114), D134 (≠ I115), E135 (≠ K116), Y167 (= Y140), N196 (≠ L169), K206 (= K184), L207 (= L185), P222 (= P193), Y224 (≠ F194), R231 (= R201), N266 (≠ T237), R297 (≠ K271), H300 (≠ M274)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), F11 (= F11), I12 (≠ L12), D32 (= D24), K33 (≠ R25), L34 (≠ I26), T35 (= T27), G38 (≠ C30), D58 (= D48), L80 (≠ A68), A81 (= A69), A82 (= A70), S84 (≠ A72), T99 (≠ Y86), S132 (= S113), T133 (≠ S114), Y167 (= Y140), K171 (= K144), C194 (≠ P167), N196 (≠ L169), N197 (≠ V170)

Sites not aligning to the query:

binding 2'deoxy-thymidine-5'-diphospho-alpha-d-glucose: 357

P26391 dTDP-glucose 4,6-dehydratase; EC 4.2.1.46 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see 2 papers)
22% identity, 99% coverage: 1:310/313 of query aligns to 1:332/361 of P26391

query
sites
P26391

M

M

N

K

V

I

L

L

L

I

T

T

G

G

A

G

N

A

G

G

F
|
F

L
x
I

G

G

R

S

A

A

I

V

V

V

A

R

H

H

L

I

C

I

R

K

Q

N

-

T

-

Q

-

D

-

T

-

V

-

N

-

I

D
|
D

R
x
K

I
x
L

T
|
T

L

Y

S

A

C

G

A

N

V

L

R

E

-

S

-

L

S

S

P

D

L

I

A

S

Q

E

V

S

R

N

F

R

A

Y

T

N

F

F

A

E

V

H

G

A

D
|
D

L
x
I

C

C

G

D

A

S

N

A

D

E

W

I

S

T

Q

R

P

I

L

F

L

E

G

Q

Q

Y

Q

Q

-

P

-

D

V

A

V

V

I

M

H

H

A
x
L

A
|
A

R
x
E

A
x
S

H

H

I

V

M

D

K

R

D

-

E

S

L

I

A

T

D

G

P

P

L

A

S

A

-

F

-

I

E

E

Y
x
T

R

N

L

I

V

V

N

G

V

T

E

Y

G

A

T

L

L

L

N

E

L

V

A

A

R

R

Q

K

A

Y

A

W

A

S

A

A

G

L

V

G

E

E

-

D

-

K

-

N

-

F

R

R

F

F

I

H

Y

H

I

I

S

S

S
x
T

I

D

K

E

V

V

N

Y

G

G

E

D

-

L

-

P

-

H

-

P

-

D

-

E

-

V

-

E

-

N

-

S

-

V

S

T

T

L

P

P

L

L

G

-

K

-

P

-

F

F

V

T

S

E

S

T

D

T

A

A

P

Y

A

A

P

P

E

S

D

S

P

P

Y
|
Y

G
x
S

L
x
A

S
|
S

K
|
K

L

A

E

S

A

S

E

D

Q

H

G

L

L

V

M

R

Q

A

L

W

A

R

E

T

T

Y

G

G

M

L

E

P

V

T

V

I

I

V

I

T

R

N

P

C

P

S

L
x
N

V
x
N

Y

Y

G

G

P

P

G

-

V

-

K

-

G

-

N

-

F

Y

A

H

S

F

M

P

I

E

K
|
K

L
|
L

I

I

D

P

-

L

-

V

-

I

-

L

-

N

-

A

-

L

R

E

G

G

I

K

P

P

L

L

P
|
P

-
x
I

F
x
Y

G

G

A

K

I

G

H

D

N

Q

K

I

R
|
R

S

D

L

W

V

L

G

Y

V

V

D

E

N

D

L

H

V

A

D

R

L

A

I

L

I

H

R

M

C

V

V

V

D

T

H

-

P

E

A

G

A

K

A

A

N

G

Q

E

I

T

F

Y

L

N

A

I

G

G

D

G

G

H

K

N

D

E

L

K

S

K

T
x
N

T

L

E

D

L

V

L

F

G

T

V

I

G

C

K

D

A

L

M

L

D

D

K

-

P

-

A

-

K

E

L

I

I

V

P

P

A

K

P

A

A

T

G

S

F

Y

L

R

Q

E

L

Q

G

I

A

T

T

Y

L

V

L

A

G
x
D

K
x
R

K
x
P

A
x
G

M
x
H

A

D

Q

R

R

R

L

-

G

-

S

-

L

Y

Q

A

V

I

D

D

I

A

S

G

K

K

T

I

C

S

E

R

L

E

L

L

D

G

W

W

K

K

P

P

P

L

Y

E

T

T

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F

E

E

E

S

G

G

L

I

R

R

R

K

C

T

F

V

E

E

FI 11:12 (≠ FL 11:12) binding
DKLT 32:35 (≠ DRIT 24:27) binding
DI 58:59 (≠ DL 48:49) binding
LAAES 80:84 (≠ AAARA 68:72) binding
S84 (≠ A72) binding
T99 (≠ Y86) binding
T133 (≠ S114) binding
YSASK 167:171 (≠ YGLSK 140:144) binding
N196 (≠ L169) binding
N197 (≠ V170) binding
KL 206:207 (= KL 184:185) binding
PIY 222:224 (≠ P-F 193:194) binding
R231 (= R201) binding
N266 (≠ T237) binding
DRPGH 296:300 (≠ GKKAM 270:274) binding

Sites not aligning to the query:

357 binding

Q9FX01 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1; At3BETAHSD/D1; 4alpha-carboxysterol-C3-dehydrogenase/C4-decarboxylase isoform 1-1; Reticulon-like protein B24; AtRTNLB24; Sterol-4-alpha-carboxylate 3-dehydrogenase 1, decarboxylating; EC 1.1.1.418 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
26% identity, 98% coverage: 4:310/313 of query aligns to 13:356/439 of Q9FX01

query
sites
Q9FX01

L

V

T

T

G

G

A

G

N

R

G

G

F

F

L

A

G

A

R

R

A

H

I

L

V

V

A

E

H

M

L

L

C

V

R

R

Q

Y

D

Q

-

M

-

F

-

H

-

V

R

R

I

I

T

A

-
x
D

-

L

-

A

-

P

-

A

-

I

-

V

-

L

-

N

-

P

-

H

-

E

-

T

-

G

-

I

L

L

S

G

C

E

A

A

V

I

R

R

S

S

P

G

L

R

A

V

Q

Q

V

-

R

-

F

-

A

-

T

-

F

Y

A

V

V

S

G

A

D
|
D

L

L

C

R

G

N

A

K

N

T

D

Q

W

V

S

V

Q

K

P

G

L

F

L

Q

G

G

Q

A

Q

E

V

V

I

F

H

H

A

M

A

A

-

P

R

D

A

S

H

S

I

I

M

N

K

N

D

H

E

Q

L

L

A

-

D

-

P

-

L

-

S

-

E

Q

Y

Y

R

S

L

-

V

V

N

N

V

V

E

Q

G

G

T

T

L

T

N

N

L

V

A

I

R

D

Q

A

A

C

A

I

A

E

A

V

G

G

V

V

E

K

R

R

F

L

I

I

Y

Y

I
x
T

S

S

S
|
S

I

P

K
x
S

V

V

-

V

-

F

N

D

G

G

E

V

S

H

T

G

P

T

L

L

G

N

K

A

P

D

F

E

V

S

S

L

S

P

D

Y

A

P

P

P

A

K

P

H

E

N

D

D

P

S

Y
|
Y

G

S

L

A

S

T

K
|
K

L

A

E

E

A

G

E

E

Q

A

G

L

L

I

M

L

Q

K

L

A

A

N

A

G

E

R

T

S

G

G

M

L

E

L

V

T

V

C

I

C

I

I

R

R

P

P

P

S

L

S

V

I

Y

F

G

G

P

P

G

G

V

D

K

K

G

L

N

M

F

V

A

P

S

S

M

L

I

V

K

T

L

A

I

A

D

R

R

A

G

G

-

K

I

S

P

K

L

F

P

I

F

I

G

G

A

D

I

G

H

S

N

N

K

F

R

Y

S

D

L

F

V

T

G

Y

V

V

D

E

N

N

L

V

V

V

D

H

L

A

I

H

I

V

R

-

C

C

V

A

D

E

H

R

-

A

-

L

-

A

-

S

-

G

-

E

-

V

-

C

P

A

A

K

A

A

N

G

Q

Q

I

A

F

Y

L

F

A

I

G

T

D

N

G

M

K

E

D

P

L

I

S

K

T

F

T

W

E

E

L

F

L

M

-

S

-

Q

L

L

G

L

V

E

G

G

K

L

A

G

M

Y

D

E

K

R

P

P

A

S

K

I

L

K

I

I

P

P

A

A

-

S

-

L

-

M

P

P

A

I

G

A

F

Y

L

L

-

V

Q

E

L

L

G

A

A

Y

T

K

L

L

G

G

K

P

K

Y

A

G

M

M

A

K

Q

V

R

P

L

V

L

L

G

T

S

P

L

S

Q
x
R

V

V

-

R

-

L

-

S

-

C

-

N

-
x
R

-

T

-

F

D

D

I

S

S

S

K

K

T

A

C

K

E

D

L

R

L

L

D

G

W

Y

K

S

P

P

P

V

Y

V

T

P

V

L

E

Q

E

E

G

G

L

I

R

K

R

R

C

T

F

I

E

D

D41 (vs. gap) Essential for the 3betaHSD/D activity; mutation to V: Lost activity.
D72 (= D48) Essential for the 3betaHSD/D activity; mutation D->A,V: Lost activity.
T131 (≠ I112) Essential for the 3betaHSD/D activity; mutation to V: Lost activity.
S133 (= S114) mutation to A: Reduced activity with slower catalysis and lower substrate binding.
S135 (≠ K116) mutation to A: Normal activity, but slower catalysis with 4alpha-carboxysterol as substrate.; mutation to T: Altered activity due to reduced affinity and catalysis and leading to lower amount of C4-demethylated sterols and higher quantities of 4,4-dimethylated sterol intermediates, and a higher ratio of 4,4-dimethylsterols to 4alpha-methylsterols.; mutation to Y: Altered activity leading to an exclusive production of C4-methylated sterols and 4alpha-carboxy-3beta-hydroxy sterols.
Y161 (= Y140) Essential for the 3betaHSD/D activity; mutation to F: Lost activity.
K165 (= K144) Essential for the 3betaHSD/D activity; mutation to I: Lost activity.
R320 (≠ Q283) mutation to I: Normal activity.
R328 (vs. gap) Essential for the 3betaHSD/D activity; mutation to I: Lost activity.

P27830 dTDP-glucose 4,6-dehydratase 2; EC 4.2.1.46 from Escherichia coli (strain K12) (see 2 papers)
23% identity, 98% coverage: 3:310/313 of query aligns to 4:329/355 of P27830

query
sites
P27830

V

I

L

L

L

I

T

T

G

G

A

G

N

A

G

G

F
|
F

L
x
I

G

G

R

S

A

A

I

L

V

V

A

R

H

Y

L

I

C

I

R

N

Q

E

-

T

-

S

-

D

-

A

-

V

D
|
D

R
x
K

I
x
L

T
|
T

L

Y

S

A

C

G

A

N

V

L

R

M

S

S

-

L

-

A

P

P

L

V

A

A

Q

Q

V

S

R

E

F

R

A

F

T

A

F

F

A

E

V

K

G

V

D
|
D

L
x
I

C

C

G

D

A

R

N

A

D

E

W

L

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A

Q

R

P

V

L

F

L

T

G

E

Q

H

Q

Q

-

P

-

D

V

C

V

V

I

M

H

H

A

L

A

A

R

E

A

S

H

H

I

V

M

-

K

-

D

D

E

R

L

S

A

I

D

D

P

G

L

P

S

A

E

A

Y

F

R

I

L

E

V
x
T

N

N

V

I

E

V

G

G

T

T

L

Y

N

T

L

L

-

L

-

E

-

A

A

A

R

R

Q

A

A

Y

A

W

A

N

A

A

G

L

V

T

E

E

-

D

-

K

-

S

-

A

-

F

R

R

F

F

I

H

Y

H

I

I

S

S

S

T

I
x
D

K
x
E

V

V

N

Y

G

G

E

D

S

L

T

H

P

S

L

T

G

D

K

D

P

F

F

F

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T

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E

S

T

D

T

A

P

P

Y

A

A

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P

E

S

D

S

P

P

Y
|
Y

G
x
S

L
x
A

S
|
S

K
|
K

L

A

E

S

A

S

E

D

Q

H

G

L

L

V

M

R

Q

A

L

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A

L

A

R

E

T

T

Y

G

G

M

L

E

P

V

T

V

L

I

I

I

T

R

N

P

C

P

S

L

N

V
x
N

Y

Y

G

G

P

P

G

-

V

-

K

-

G

Y

N

H

F

F

A

P

S

E

-

K

-

L

-

I

-

P

-

L

M

M

I

I

K

L

L

N

I

A

D

L

R

A

G

G

I

K

P

S

L

L

P

P

-

V

F

Y

G

G

A

N

I

G

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Q

N

Q

K

I

R

R

S

D

L

W

V

L

G

Y

V

V

D

E

N

D

L

H

V

A

D

R

L

A

I

L

I

Y

R

-

C

C

V

V

D

A

H

T

P

T

A

G

A

K

A

V

N

G

Q

E

I

T

F

Y

L

N

A

I

G

G

D

G

G

H

K

N

D

E

L

R

S

K

T

N

T

L

E

D

L

V

L

E

G

T

V

I

G

C

K

E

A

L

M

L

D

E

K

E

P

L

A

A

K

P

L

N

I

K

P

P

A

H

P

G

A

V

G

A

F

H

L

Y

Q

R

L

D

G

L

A

I

T

T

L

F

L

V

G

A

K

D

K

R

A

P

M

G

A

H

Q

D

R

L

L

R

L

Y

G

A

S

-

L

-

Q

-

V

I

D

D

I

A

S

S

K

K

T

I

C

A

E

R

L

E

L

L

D

G

W

W

K

L

P

P

P

Q

Y

E

T

T

V

F

E

E

E

S

G

G

L

M

R

R

R

K

C

T

F

V

E

Q

FI 12:13 (≠ FL 11:12) binding
DKLT 33:36 (≠ DRIT 24:27) binding
DI 59:60 (≠ DL 48:49) binding
T100 (≠ V89) binding
D135 (≠ I115) active site, Proton donor
E136 (≠ K116) active site, Proton acceptor
Y160 (= Y140) active site, Proton acceptor
YSASK 160:164 (≠ YGLSK 140:144) binding
N190 (≠ V170) binding

6kv9A Moee5 in complex with udp-glucuronic acid and NAD (see paper)
29% identity, 77% coverage: 2:241/313 of query aligns to 2:239/299 of 6kv9A

query
sites
6kv9A

N

D

V

V

L

L

L

V

T

T

G
|
G

A

A

N

A

G
|
G

F
|
F

L
x
I

G

G

R

S

A

H

I

L

V

V

A

T

H

E

L

L

C

R

R

N

Q

S

D

G

R

R

I

N

T

V

L

V

S

A

C

V

A
x
D

V

-

R
|
R

S
x
R

P

P

L

L

A

P

Q

D

V

T

R

G

F

S

A

L

T

R

F

E

A

I

V

R

G

G

D
|
D

L
|
L

C

N

G

S

A

L

N

N

D

-

W

L

S

V

Q

D

P

C

L

L

L

K

G

N

Q

I

Q

S

V

T

V

V

I

F

H

H

A
x
L

A
|
A

R

L

A
x
P

H

G

I

V

M
x
R

K

P

D

S

E

W

L

T

A

Q

D

F

P

P

L

-

S

-

E

E

Y

Y

R

L

L

R

V
x
C

N

N

V

V

E

L

G

A

T

T

L

Q

N

R

L

L

A

M

R

E

Q

A

A

C

A

V

A

Q

A

A

G

G

V

V

E

E

R

R

F

V

I

V

Y

V

I
x
A

S
|
S

I
x
S

K
x
S

V

V

N

Y

G

G

E

G

S

A

T

D

P

G

L

V

G

-

K

-

P

-

F

M

V

S

S

E

S

D

D

D

A

L

P

P

A

R

P

P

E

L

D

S

P

P

Y
|
Y

G

G

L

V

S

T

K
|
K

L

L

E

A

A

A

E

E

Q

R

G

L

L

A

M

L

Q

A

L

F

A

A

E

R

T

G

G

D

M

A

E

E

V

L

V

S

I

V

-

G

-

A

I

L

R

R

P
x
F

P

F

L
x
T

V
|
V

Y

Y

G

G

P

P

G

G

V

Q

K
x
R

G

P

N

D

F
x
M

-
x
F

-

I

A

S

S
x
R

M

L

I

I

K

R

L

A

I

T

D

L

R

R

G

G

I

E

P

P

L

V

P
x
E

-
x
I

F
x
Y

G

G

A

D

I

G

H

T

N
x
Q

K

L

R
|
R

S

D

L

F

V

T

G

H

V

V

D

S

N

D

L

V

V

V

D

R

L

A

I

L

I

M

R

L

C

T

V

A

D

S

H

V

P

R

A

D

A

R

A

G

N

S

Q

A

I

V

F

L

L

N

A

I

G

G

D

T

G

G

K

S

D

A

L

V

S

S

T
x
V

T

N

E

E

L

V

active site: S110 (= S114), S111 (≠ I115), S112 (≠ K116), Y133 (= Y140), K137 (= K144)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), F11 (= F11), I12 (≠ L12), D31 (≠ A31), R32 (= R33), R33 (≠ S34), D47 (= D48), L48 (= L49), L66 (≠ A68), A67 (= A69), A68 (= A70), P70 (≠ A72), C85 (≠ V89), A108 (≠ I112), S109 (= S113), K137 (= K144), F162 (≠ P167), T164 (≠ L169), V165 (= V170), R171 (≠ K176), M174 (≠ F179)
binding uridine-5'-diphosphate-glucuronic acid: P70 (≠ A72), R73 (≠ M75), S110 (= S114), S111 (≠ I115), Y133 (= Y140), T164 (≠ L169), R171 (≠ K176), M174 (≠ F179), F175 (vs. gap), R178 (≠ S181), E190 (≠ P193), I191 (vs. gap), Y192 (≠ F194), Q197 (≠ N199), R199 (= R201), V235 (≠ T237)

Sites not aligning to the query:

binding uridine-5'-diphosphate-glucuronic acid: 261

1udaA Structure of udp-galactose-4-epimerase complexed with udp-4-deoxy-4- fluoro-alpha-d-galactose (see paper)
32% identity, 53% coverage: 1:166/313 of query aligns to 1:176/338 of 1udaA

query
sites
1udaA

M

M

N

R

V

V

L

L

L

V

T

T

G
|
G

A

G

N

S

G
|
G

F
x
Y

L
x
I

G

G

R

-

A

-

I

-

V

-

A

S

H

H

L

T

C

C

R

V

Q

Q

-

L

-

Q

-

N

-

G

-

H

D

D

R

V

I

I

T

I

L

L

S
x
D

-
x
N

-
x
L

C
|
C

A
x
N

V
x
S

R

K

S

R

P

S

L

V

A

L

Q

P

V

V

-

I

-

E

-

R

-

L

-

G

R

K

F

H

A

P

T

T

F

F

A

V

V

E

G

G

D
|
D

L
x
I

C

R

G

N

A

E

N

A

D

L

W

M

S

T

Q

E

P

I

L

L

L

H

G

D

Q

H

Q

A

V

I

-

D

-

T

V

V

I

I

H

H

A
x
F

A
|
A

A
x
G

R

L

A
x
K

H

A

I

V

M

-

K

G

D

E

E

S

L

V

A

Q

D

K

P

P

L

L

S

-

E

E

Y

Y

R

Y

L

D

V

N

N

N

V

V

E

N

G

G

T

T

L

L

N

R

L

L

A

I

R

S

Q

A

A

M

A

R

A

A

G

N

V

V

E

K

R

N

F

F

I

I

Y

F

I
x
S

S

S

S
|
S

I
x
A

K
x
T

V

V

N

Y

G

G

E

D

S

N

T

P

P

K

L

I

G

-

K

-

P

P

F

Y

V

V

S

E

S

S

-

F

D

P

A

T

P

G

A

T

P

P

E

Q

D

S

P

P

Y
|
Y

G

G

L

K

S

S

K
|
K

L

L

E

M

A

V

E

E

Q

Q

G

I

L

L

M

T

Q

D

L

L

-

Q

A

K

A

A

E

Q

T

P

G

D

M

W

E

S

V

I

V

A

I

L

R

R

active site: S124 (= S114), A125 (≠ I115), T126 (≠ K116), Y149 (= Y140), K153 (= K144)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (≠ L12), D31 (≠ S29), N32 (vs. gap), L33 (vs. gap), C34 (= C30), N35 (≠ A31), S36 (≠ V32), D58 (= D48), I59 (≠ L49), F80 (≠ A68), A81 (= A69), G82 (≠ A70), K84 (≠ A72), S122 (≠ I112), Y149 (= Y140), K153 (= K144)
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: T126 (≠ K116)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-5'-diphosphate-4-deoxy-4-fluoro-alpha-d-galactose: 179, 199, 200, 216, 217, 218, 231, 233, 292, 295, 299

1naiA Udp-galactose 4-epimerase from escherichia coli, oxidized (see paper)
32% identity, 53% coverage: 1:166/313 of query aligns to 1:176/338 of 1naiA

query
sites
1naiA

M

M

N

R

V

V

L

L

L

V

T

T

G
|
G

A

G

N

S

G
|
G

F
x
Y

L
x
I

G

G

R

-

A

-

I

-

V

-

A

S

H

H

L

T

C

C

R

V

Q

Q

-

L

-

Q

-

N

-

G

-

H

D

D

R

V

I

I

T

I

L

L

S
x
D

-
x
N

-
x
L

C
|
C

A
x
N

V
x
S

R

K

S

R

P

S

L

V

A

L

Q

P

V

V

-

I

-

E

-

R

-

L

-

G

R

K

F

H

A

P

T

T

F

F

A

V

V

E

G

G

D
|
D

L
x
I

C

R

G

N

A

E

N

A

D

L

W

M

S

T

Q

E

P

I

L

L

L

H

G

D

Q

H

Q

A

V

I

-

D

-

T

V

V

I

I

H

H

A
x
F

A
|
A

A
x
G

R

L

A
x
K

H

A

I

V

M

-

K

G

D

E

E

S

L

V

A

Q

D

K

P

P

L

L

S

-

E

E

Y

Y

R

Y

L

D

V

N

N

N

V

V

E

N

G

G

T

T

L

L

N

R

L

L

A

I

R

S

Q

A

A

M

A

R

A

A

G

N

V

V

E

K

R

N

F

F

I

I

Y

F

I

S

S

S

S
|
S

I
x
A

K
x
T

V

V

N

Y

G

G

E

D

S

N

T

P

P

K

L

I

G

-

K

-

P

P

F

Y

V

V

S

E

S

S

-

F

D

P

A

T

P

G

A

T

P

P

E

Q

D

S

P

P

Y
|
Y

G

G

L

K

S

S

K
|
K

L

L

E

M

A

V

E

E

Q

Q

G

I

L

L

M

T

Q

D

L

L

-

Q

A

K

A

A

E

Q

T

P

G

D

M

W

E

S

V

I

V

A

I

L

R

R

active site: S124 (= S114), A125 (≠ I115), T126 (≠ K116), Y149 (= Y140), K153 (= K144)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (≠ L12), D31 (≠ S29), N32 (vs. gap), L33 (vs. gap), C34 (= C30), N35 (≠ A31), S36 (≠ V32), D58 (= D48), I59 (≠ L49), F80 (≠ A68), A81 (= A69), G82 (≠ A70), K84 (≠ A72), Y149 (= Y140), K153 (= K144)
binding 1,3-propandiol: N35 (≠ A31), K84 (≠ A72)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding 1,3-propandiol: 191, 193
binding uridine-5'-diphosphate: 179, 199, 200, 215, 216, 231, 233, 292

1lrjA Crystal structure of e. Coli udp-galactose 4-epimerase complexed with udp-n-acetylglucosamine (see paper)
32% identity, 53% coverage: 1:166/313 of query aligns to 1:176/338 of 1lrjA

query
sites
1lrjA

M

M

N

R

V

V

L

L

L

V

T

T

G
|
G

A

G

N

S

G
|
G

F
x
Y

L
x
I

G

G

R

-

A

-

I

-

V

-

A

S

H

H

L

T

C

C

R

V

Q

Q

-

L

-

Q

-

N

-

G

-

H

D

D

R

V

I

I

T

I

L

L

S
x
D

-
x
N

-
x
L

C
|
C

A
x
N

V
x
S

R

K

S

R

P

S

L

V

A

L

Q

P

V

V

-

I

-

E

-

R

-

L

-

G

R

K

F

H

A

P

T

T

F

F

A

V

V

E

G

G

D
|
D

L
x
I

C

R

G

N

A

E

N

A

D

L

W

M

S

T

Q

E

P

I

L

L

L

H

G

D

Q

H

Q

A

V

I

-

D

-

T

V

V

I

I

H

H

A
x
F

A
|
A

A
x
G

R

L

A
x
K

H

A

I
x
V

M

-

K

G

D

E

E

S

L

V

A

Q

D

K

P

P

L

L

S

-

E

E

Y

Y

R

Y

L

D

V

N

N

N

V

V

E

N

G

G

T

T

L

L

N

R

L

L

A

I

R

S

Q

A

A

M

A

R

A

A

G

N

V

V

E

K

R

N

F

F

I

I

Y

F

I

S

S

S

S
|
S

I
x
A

K
x
T

V

V

N

Y

G

G

E

D

S

N

T

P

P

K

L

I

G

-

K

-

P

P

F

Y

V

V

S

E

S

S

-

F

D

P

A

T

P

G

A

T

P

P

E

Q

D

S

P

P

Y
|
Y

G

G

L

K

S

S

K
|
K

L

L

E

M

A

V

E

E

Q

Q

G

I

L

L

M

T

Q

D

L

L

-

Q

A

K

A

A

E

Q

T

P

G

D

M

W

E

S

V

I

V

A

I

L

R

R

active site: S124 (= S114), A125 (≠ I115), T126 (≠ K116), Y149 (= Y140), K153 (= K144)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (≠ L12), D31 (≠ S29), N32 (vs. gap), L33 (vs. gap), C34 (= C30), N35 (≠ A31), S36 (≠ V32), D58 (= D48), I59 (≠ L49), F80 (≠ A68), A81 (= A69), G82 (≠ A70), K84 (≠ A72), Y149 (= Y140), K153 (= K144)
binding uridine-diphosphate-n-acetylglucosamine: V86 (≠ I74), S124 (= S114)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177, 180
binding uridine-diphosphate-n-acetylglucosamine: 178, 179, 200, 215, 216, 218, 231, 233, 269, 292, 295

G4MVZ2 Polyketide synthase-nonribosomal peptide synthetase ACE1; PKS-NRPS ACE1; ACE1 cytochalasan biosynthesis cluster protein ACE1; Avirulence conferring enzyme; EC 2.3.1.-; EC 6.3.2.- from Pyricularia oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Magnaporthe oryzae) (see paper)
31% identity, 68% coverage: 3:215/313 of query aligns to 3717:3948/4034 of G4MVZ2

query
sites
G4MVZ2

V

V

L

L

T

T

G

G

A

A

N

T

G

G

F

F

L

L

G

G

R

R

A

Q

I

L

V

M

A

A

H

F

L

L

C

L

R

R

Q

Q

D

P

R

S

I

V

T

K

-

R

L

I

S

H

C

C

-

L

A

A

V

V

R

R

-

G

-

A

-

P

-

S

-

A

S

A

P

P

L

F

A

S

Q

D

V

P

R

R

F

V

A

S

T

I

F

H

A

A

V

-

G

G

D

D

L

L

-

N

-

A

-

P

-

H

C

L

G

G

A

L

N

G

D

E

W

A

S

V

Q

A

P

E

L

L

G

F

Q

A

Q

Q

-

A

-

D

V

V

V

I

I

I

H

H

A

N

A

G

A

A

R

D

A

V

H

S

I

F

M

L

K

K

D

T

E

-

L

-

A

-

D

-

P

-

L

Y

S

A

E

T

Y

L

R

R

L

A

V

T

N

N

V

V

E

G

G

S

T

T

L

R

N

E

L

L

A

A

R

R

Q

L

A

A

A

P

A

R

G

R

V

I

E

P

R

-

F

F

I

H

Y

F

I

V

S

S

I

A

K

S

V

I

N

T

G

Q

E

L

S

T

-

G

-

L

T

D

P

E

L

F

G

G

K

E

P

A

F

S

V

M

S

A

D

W

A

A

P

P

A

-

P

P

E

A

D

D

P

P

Y

R

G

G

L

M

S

S

-

G

-

Y

-

A

K

K

L

W

E

A

A

S

E

E

Q

V

G

L

L

L

M

E

Q

K

L

A

A

A

A

R

E

A

T

W

G

G

M

L

E

P

V

V

I

I

I

H

R

R

P

P

P

S

L

S

V

I

Y

T

G

G

P

E

G

G

V

T

K

N

G

S

-

L

-

D

N

L

F

M

A

G

S

N

M

M

I

F

K

K

L

Y

I

I

D

E

R

Q

G

L

I

E

P

A

L

V

P

P

F

E

G

S

-

D

A

S

I

W

H

K

N

G

K

N

R

F

S

D

L

F

V

V

G

S

V

V

D

E

N

N

L

V

V

A

D

A

L

D

I

I

I

V

R

Q

Sites not aligning to the query:

183 C→A: Abolishes recognition of the fungus by resistant rice plants.

2udpA Udp-galactose 4-epimerase complexed with udp-phenol (see paper)
32% identity, 53% coverage: 1:166/313 of query aligns to 1:176/338 of 2udpA

query
sites
2udpA

M

M

N

R

V

V

L

L

L

V

T

T

G
|
G

A

G

N

S

G
|
G

F
x
Y

L
x
I

G

G

R

S

A

H

I

T

V

C

A

V

H

Q

L

L

C

L

R

Q

-

N

-

G

Q

H

D

D

R

V

I

I

T

I

L

L

S
x
D

-
x
N

-
x
L

C
|
C

A
x
N

V
x
S

R

K

S

R

P

S

L

V

A

L

Q

P

V

V

-

I

-

E

-

R

-

L

-

G

R

K

F

H

A

P

T

T

F

F

A

V

V

E

G

G

D
|
D

L
x
I

C

R

G

N

A

E

N

A

D

L

W

M

S

T

Q

E

P

I

L

L

L

H

G

D

Q

H

Q

A

V

I

-

D

-

T

V

V

I

I

H

H

A
x
F

A
|
A

A
x
G

R

L

A
x
K

H

A

I

V

M

-

K

G

D

E

E

S

L

V

A

Q

D

K

P

P

L

L

S

-

E

E

Y

Y

R

Y

L

D

V

N

N

N

V

V

E

N

G

G

T

T

L

L

N

R

L

L

A

I

R

S

Q

A

A

M

A

R

A

A

G

N

V

V

E

K

R

N

F

F

I

I

Y

F

I
x
S

S

S

S
|
S

I
x
A

K
x
T

V

V

N

Y

G

G

E

D

S

Q

T

P

P

K

L

I

G

-

K

-

P

P

F

Y

V

V

S

E

S

S

-

F

D

P

A

T

P

G

A

T

P

P

E

Q

D

S

P

P

Y
|
Y

G

G

L

K

S

S

K
|
K

L

L

E

M

A

V

E

E

Q

Q

G

I

L

L

M

T

Q

D

L

L

-

Q

A

K

A

A

E

Q

T

P

G

D

M

W

E

S

V

I

V

A

I

L

R

R

active site: S124 (= S114), A125 (≠ I115), T126 (≠ K116), Y149 (= Y140), K153 (= K144)
binding nicotinamide-adenine-dinucleotide: G7 (= G7), G10 (= G10), Y11 (≠ F11), I12 (≠ L12), D31 (≠ S29), N32 (vs. gap), L33 (vs. gap), C34 (= C30), N35 (≠ A31), S36 (≠ V32), D58 (= D48), I59 (≠ L49), F80 (≠ A68), A81 (= A69), G82 (≠ A70), K84 (≠ A72), S122 (≠ I112), S124 (= S114), Y149 (= Y140), K153 (= K144)

Sites not aligning to the query:

active site: 189
binding nicotinamide-adenine-dinucleotide: 177
binding phenyl-uridine-5'-diphosphate: 179, 199, 200, 216, 217, 218, 231, 233, 269, 292, 295

Query Sequence

>GFF1784 FitnessBrowser__psRCH2:GFF1784
MNVLLTGANGFLGRAIVAHLCRQDRITLSCAVRSPLAQVRFATFAVGDLCGANDWSQPLL
GQQVVIHAAARAHIMKDELADPLSEYRLVNVEGTLNLARQAAAAGVERFIYISSIKVNGE
STPLGKPFVSSDAPAPEDPYGLSKLEAEQGLMQLAAETGMEVVIIRPPLVYGPGVKGNFA
SMIKLIDRGIPLPFGAIHNKRSLVGVDNLVDLIIRCVDHPAAANQIFLAGDGKDLSTTEL
LLGVGKAMDKPAKLIPAPAGFLQLGATLLGKKAMAQRLLGSLQVDISKTCELLDWKPPYT
VEEGLRRCFEPVK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory