SitesBLAST
Comparing GFF1835 FitnessBrowser__Marino:GFF1835 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
63% identity, 99% coverage: 5:1229/1232 of query aligns to 2:1225/1227 of P13009
- C247 (= C250) binding
- C310 (= C313) binding ; mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- C311 (= C314) binding ; mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- E694 (= E694) binding
- GDVHD 756:760 (= GDVHD 757:761) binding
- D757 (= D758) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
- H759 (= H760) binding axial binding residue; mutation to G: Loss of catalytic activity.
- S804 (= S805) binding
- T808 (= T809) binding
- S810 (= S811) mutation to A: Decreases activity by about 40%.
- A860 (= A862) binding
- D946 (= D949) binding
- R1134 (= R1138) binding
- YY 1189:1190 (≠ YF 1193:1194) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
55% identity, 100% coverage: 4:1229/1232 of query aligns to 15:1263/1265 of Q99707
- R61 (= R50) natural variant: R -> K
- C255 (≠ S245) to Y: in dbSNP:rs1140598
- GSR 382:384 (≠ GSK 370:372) binding
- D449 (= D437) binding
- N470 (= N458) binding
- D537 (= D524) binding
- N579 (= N566) binding
- R585 (= R572) binding
- R591 (= R578) binding
- D919 (≠ R895) to G: in dbSNP:rs1805087
- D963 (≠ E938) mutation to E: Decreases binding to MTRR; when associated with N-1071.
- K1071 (= K1038) mutation to N: Decreases binding to MTRR; when associated with E-963.
3ivaA Structure of the b12-dependent methionine synthase (meth) c-teminal half with adohcy bound (see paper)
61% identity, 47% coverage: 654:1229/1232 of query aligns to 4:575/576 of 3ivaA
- active site: D107 (= D758), H109 (= H760), S160 (= S811)
- binding cobalamin: H109 (= H760), G112 (= G763), V116 (= V767), G152 (= G803), L153 (= L804), S154 (= S805), L156 (= L807), I157 (= I808), T158 (= T809), G183 (= G834), G184 (= G835), Q208 (≠ S860), N209 (≠ D861), T303 (≠ S956), D443 (= D1097), A486 (= A1140), G488 (= G1142), Y489 (= Y1143), H495 (= H1149), A520 (= A1174), M521 (= M1175), G524 (≠ T1178), V527 (= V1181), S528 (= S1182)
- binding s-adenosyl-l-homocysteine: E447 (= E1101), R484 (= R1138), P485 (= P1139), Y489 (= Y1143), A491 (= A1145), Y539 (= Y1193)
3bulA E. Coli i690c/g743c meth c-terminal fragment (649-1227) (see paper)
61% identity, 47% coverage: 654:1229/1232 of query aligns to 4:575/577 of 3bulA
- active site: D107 (= D758), H109 (= H760), S160 (= S811)
- binding cobalamin: H109 (= H760), V116 (= V767), G152 (= G803), L153 (= L804), S154 (= S805), L156 (= L807), I157 (= I808), T158 (= T809), G183 (= G834), G184 (= G835), Q208 (≠ S860), N209 (≠ D861), A210 (= A862), T213 (≠ C865), M302 (= M955), D443 (= D1097), A486 (= A1140), P487 (= P1141), G488 (= G1142), Y489 (= Y1143), H495 (= H1149), K498 (= K1152), M521 (= M1175), G524 (≠ T1178), V527 (= V1181), S528 (= S1182)
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
54% identity, 51% coverage: 6:638/1232 of query aligns to 1:611/611 of 4cczA
- binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E364), G342 (= G370), R344 (≠ K372), N430 (= N458), M458 (= M486), D497 (= D524), G536 (= G563), S538 (= S565), N539 (= N566), F542 (= F569), R545 (= R572), R551 (= R578)
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
36% identity, 67% coverage: 13:843/1232 of query aligns to 7:806/841 of 8g3hA