SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1871 FitnessBrowser__Marino:GFF1871 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

1nsjA Crystal structure of phosphoribosyl anthranilate isomerase from thermotoga maritima (see paper)
41% identity, 94% coverage: 4:200/209 of query aligns to 3:200/205 of 1nsjA

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active site: C7 (= C8), D126 (= D127)
binding phosphate ion: G133 (= G135), V179 (= V179), S180 (= S180), S181 (≠ G181)

1lbmA Crystal structure of phosphoribosyl anthranilate isomerase (prai) in complex with reduced 1-(o-carboxyphenylamino)-1-deoxyribulose 5- phosphate (rcdrp) (see paper)
38% identity, 95% coverage: 3:200/209 of query aligns to 2:189/194 of 1lbmA

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active site: C7 (= C8), D126 (= D127)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: C7 (= C8), S34 (= S35), R36 (= R37), H83 (= H84), D126 (= D127), G147 (= G158), G148 (= G159), D167 (= D178), S169 (= S180), S170 (≠ G181)

1piiA Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: indoleglycerolphosphate synthase from escherichia coli refined at 2.0 angstroms resolution (see paper)
30% identity, 89% coverage: 6:191/209 of query aligns to 258:438/452 of 1piiA

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1piiA

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active site: C260 (= C8), D379 (= D127)
binding phosphate ion: G386 (= G138), G407 (= G159), N427 (≠ S180), S428 (≠ G181)

Sites not aligning to the query:

active site: 53, 55, 114, 163, 184, 214, 215
binding phosphate ion: 55, 215, 235, 236, 237

Query Sequence

>GFF1871 FitnessBrowser__Marino:GFF1871
MSARVKICGLTRPEDIDGAVQFGADALGLVFYEPSPRSVSLDQAAELAKRVPAFMSVVGL
FVNPSRQEVEAVLERVPLDLLQFHGDESADFCASFGRRWIKAIRVREAGQIEAAFEEFHN
ASGLLVDAWDPDRYGGTGKSFNWDLIPDYRPLPIILAGGLSSDNVFAAVKQVKPWAVDVS
GGVEQDKGIKDIQKISHFIKEVHRVCKTD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory