SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1879 FitnessBrowser__Phaeo:GFF1879 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

P48636 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; AMP nucleosidase; PaLOG; EC 3.2.2.n1; EC 3.2.2.4 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
46% identity, 99% coverage: 1:178/180 of query aligns to 1:178/195 of P48636

query
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P48636

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E74 (= E74) binding
M89 (= M89) mutation to A: Almost complete loss of activity.
R92 (= R92) mutation to A: Almost complete loss of activity.
RK 92:93 (= RK 92:93) binding
109:115 (vs. 109:115, 57% identical) binding
T112 (≠ S112) mutation to A: Almost complete loss of activity.
E114 (≠ D114) mutation to A: Almost complete loss of activity.
E115 (= E115) mutation to A: Almost complete loss of activity.
E118 (= E118) mutation to A: Retains 8% of wild-type activity.

5zbkA Crystal structure of type-i log from pseudomonas aeruginosa pao1 in complex with amp (see paper)
45% identity, 98% coverage: 3:178/180 of query aligns to 2:177/182 of 5zbkA

query
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5zbkA

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binding adenosine monophosphate: A11 (≠ S12), E73 (= E74), M88 (= M89), R91 (= R92), K92 (= K93), G108 (= G109), G110 (= G111), T111 (≠ S112)

Q5ZC82 Cytokinin riboside 5'-monophosphate phosphoribohydrolase LOG; Protein LONELY GUY; EC 3.2.2.n1 from Oryza sativa subsp. japonica (Rice) (see paper)
45% identity, 86% coverage: 2:156/180 of query aligns to 34:188/242 of Q5ZC82

query
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Q5ZC82

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G73 (= G41) mutation to D: In LOG-4; decreased activity.

O05306 Cytokinin riboside 5'-monophosphate phosphoribohydrolase; Protein LONELY GUY homolog; LOG homolog; EC 3.2.2.n1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
43% identity, 96% coverage: 5:177/180 of query aligns to 12:183/187 of O05306

query
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O05306

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M54 (= M48) mutation to A: Large decrease in catalytic activity.
K74 (≠ E68) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup); mutation to A: Abrogates pupylation. This mutant accumulates in M.tuberculosis with a functional proteasome system and sensitizes M.tuberculosis to NO. Decrease in catalytic activity.
E80 (= E74) mutation to A: Large decrease in catalytic activity.
R98 (= R92) mutation to A: Loss of catalytic activity.
T118 (≠ S112) mutation to A: Loss of catalytic activity.
DE 120:121 (= DE 114:115) mutation to AA: Loss of catalytic activity.

7w2iA Crystal structure of log (rv1205) from mycobacterium tuberculosis (see paper)
43% identity, 96% coverage: 5:177/180 of query aligns to 8:179/183 of 7w2iA

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binding magnesium ion: D120 (≠ E118), D124 (≠ W122)

5zblD Crystal structure of type-i log from corynebacterium glutamicum in complex with amp (see paper)
40% identity, 86% coverage: 3:156/180 of query aligns to 2:154/181 of 5zblD

query
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5zblD

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binding adenosine monophosphate: S11 (= S12), M47 (= M48), R90 (= R92), K91 (= K93), G107 (= G109), G109 (= G111), T110 (≠ S112), E112 (≠ D114), E113 (= E115)

3sbxA Crystal structure of a putative uncharacterized protein from mycobacterium marinum bound to adenosine 5'-monophosphate amp (see paper)
43% identity, 96% coverage: 5:177/180 of query aligns to 3:174/177 of 3sbxA

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3sbxA

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T

G

G

F

Y

A

V

G

S

A

R

E

T

T

A

A

M

A

E

A

R

L

L

T

I

W

V

V

V

S

D

N

N

V

L

D

D

A

A

L

L

E

Q

S

A

binding adenosine monophosphate: E71 (= E74), M86 (= M89), R89 (= R92), K90 (= K93), G106 (= G109), G108 (= G111), T109 (≠ S112), D111 (= D114), E112 (= E115)

5ajuA Crystal structure of ligand-free phosphoribohydroxylase lonely guy from claviceps purpurea in complex with phosphoribose (see paper)
37% identity, 97% coverage: 5:179/180 of query aligns to 4:201/217 of 5ajuA

query
sites
5ajuA

S

K

V

I

C

C

V

V

Y

F

C

C

G

G

S
|
S

R

S

D

G

G

G

V

A

R

S

P

P

A

A

Y

H

A

M

D

E

A

A

E

R

A

Q

L

L

G

G

T

R

G

V

L

M

A

A

T

E

Q

N

G

N

Q

I

R

D

L

L

V

V

Y

Y

G

G

A

G

G

G

D

T

V

V

G

G

L

L

M

M

G

G

R

E

V

V

A

A

R

R

A

T

A

V

Q

C

A

S

A

I

G

N

G

G

-

P

-

E

R

S

T

V

F

H

G

G

V

I

I

I

P

P

E

E

H

A

L

L

V

V

R

R

R

Y

E

E

V

R

A

D

K

G

T

T

-

Y

-

Q

-

T

-

V

-

K

D

D

L

N

N

K

T

Q

Y

V

V

V

V

P

T

T

E

E

T

T

-

V

-

Y

-

G

-

R

-

T

-

V

-

K

-

D

M

M

H

H

E

T

R
|
R

K

K

V

M

M

M

L

A

Y

E

N

E

-

V

-

I

-

S

-

G

-

P

A

G

D

S

A

G

V

F

V

I

V

G

L

L

P

S

G

G

G
|
G

A
x
Y

G
|
G

S
x
T

L

M

D

E

E
|
E

L

V

F

F

E

E

A

V

L

I

T

T

W

W

R

N

Q

Q

L

L

E

G

L

I

H

H

G

T

K

K

P

G

I

I

L

C

I

L

L

L

N

N

I

V

Y

E

G

G

Y

Y

W

W

D

D

P

G

L

I

K

L

A

Q

L

W

V

I

E

N

H

M

V

A

A

A

E

Q

G

G

F

F

A

V

G

Q

A

P

E

G

T

N

A

E

A

T

A

I

L

V

T

V

W

S

V

A

S

G

N

D

V

A

D

E

D

G

A

A

L

V

E

R

S

A

L

L

R

R

binding 5-O-phosphono-beta-D-ribofuranose: S11 (= S12), R108 (= R92), G131 (= G109), Y132 (≠ A110), G133 (= G111), T134 (≠ S112), E137 (= E115)

6y01DDD p450 cytochrome, putative (see paper)
27% identity, 70% coverage: 11:136/180 of query aligns to 11:128/166 of 6y01DDD

query
sites
6y01DDD

G

G

S

P

R

G

D

E

G

Q

V

A

R

T

P

P

A

T

Y

D

A

L

D

K

A

N

A

A

E

Y

A

Q

L

L

G

G

T

Q

G

L

L

I

A

A

T

L

Q

E

G

G

Q

W

R

V

L

L

V

L

Y

T

G

G

A

G

G

R

D

N

V

V

G

G

L

V

M

M

G

E

R

H

V

A

A

S

R

Q

A

G

A

A

Q

K

A

K

A

A

G

E

G

G

R

L

T

T

F

I

G

G

V

I

I

L

P

P

E

S

H

K

L

N

V

T

R

H

R

N

E

V

V

S

A

D

K

A

T

V

D

D

L

I

N

-

T

-

Y

A

V

I

V

V

T

T

E

G

T

L

M

G

H

N

E

A

R

R

K

N

V

I

M

N

L

V

Y

L

N

S

A

S

D

D

A

V

V

V

V

I

V

A

L

C

P

G

G

I

G
|
G

A

L

G

G

S
x
T

L

L

D

S

E

E

L

V

F

A

E

L

A

A

L

L

T

K

W

-

R

-

Q

-

L

-

E

-

L

-

H

N

G

Q

K

K

P

P

I

V

L

I

I

L

L

L

N

N

binding chloride ion: G107 (= G109), T110 (≠ S112)

Query Sequence

>GFF1879 FitnessBrowser__Phaeo:GFF1879
MRIHSVCVYCGSRDGVRPAYADAAEALGTGLATQGQRLVYGAGDVGLMGRVARAAQAAGG
RTFGVIPEHLVRREVAKTDLNTYVVTETMHERKKVMLYNADAVVVLPGGAGSLDELFEAL
TWRQLELHGKPILILNIYGYWDPLKALVEHVVAEGFAGAETAAALTWVSNVDDALESLRG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory