SitesBLAST

E76 (≠ K84) binding
Y135 (≠ I144) mutation to A: 300-fold decrease in activity with spermine as substrate. Retains the ability to inhibit PrprA activity.; mutation Y->C,F: 100-fold decrease in activity with spermine as substrate.; mutation to S: Loss of activity with spermine as substrate.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
35 binding

3wr7A Crystal structure of spermidine acetyltransferase from escherichia coli (see paper)
26% identity, 56% coverage: 73:179/192 of query aligns to 61:165/170 of 3wr7A

query
sites
3wr7A

D

D

G

G

Q

E

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A

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G

S

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A

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A

A

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A

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L

L

A

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M

T

D

Y

Y

A

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F

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V

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L

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N

C

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Y

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K

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I

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N

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C

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Y

binding coenzyme a: Q83 (≠ H96), I84 (≠ T97), Q91 (= Q104), G92 (≠ K105), K93 (≠ S106), G94 (≠ A107), L95 (≠ I108), A96 (≠ N109), I119 (≠ T132), K126 (= K139), Y131 (≠ I144)

Sites not aligning to the query:

6c37A Mycobacterium smegmatis rimj in complex with coa-disulfide
29% identity, 65% coverage: 61:185/192 of query aligns to 77:198/209 of 6c37A

query
sites
6c37A

G

G

S

R

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M

I

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P

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F

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T

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A

A

A

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T

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N

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x
T

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S

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A

A
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G

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A

A

A

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H

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: G110 (= G95), Y111 (≠ H96), W112 (= W98), V113 (≠ L99), T118 (≠ Q104), G119 (≠ K105), G121 (≠ A107), A123 (≠ N109), T124 (= T110), T146 (= T132), N151 (= N137), T152 (≠ E138), P153 (≠ K139), S154 (= S140), V157 (≠ A143), H160 (≠ R146)

Sites not aligning to the query:

binding [[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(3~{R})-4-[[3-[2-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyldisulfanyl]ethylamino]-3-oxidanylidene-propyl]amino]-2,2-dimethyl-3-oxidanyl-4-oxidanylidene-butyl] hydrogen phosphate: 42, 205

6c32A Mycobacterium smegmatis rimj with accoa
29% identity, 65% coverage: 61:185/192 of query aligns to 77:198/209 of 6c32A

query
sites
6c32A

G

G

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binding acetyl coenzyme *a: G110 (= G95), Y111 (≠ H96), W112 (= W98), V113 (≠ L99), T118 (≠ Q104), G119 (≠ K105), G121 (≠ A107), A123 (≠ N109), T124 (= T110), T146 (= T132), N151 (= N137), T152 (≠ E138), P153 (≠ K139), S154 (= S140), V157 (≠ A143), H160 (≠ R146)

Sites not aligning to the query:

binding acetyl coenzyme *a: 205

Query Sequence

>GFF1879 FitnessBrowser__WCS417:GFF1879
MTLISLTGTTVELLPLQREHKTALLEAAADGELWNLKVTNVPGPDTVDKYIDTALAGRDA
GSMIPFTLVRRDDGQVVGSTRFWKVDRINRKLEIGHTWLALSTQKSAINTEAKLLLLTYA
FEVLDCVRVQFTTDELNEKSRAAILRLGAVQEGIVRHERIMPDGRKRNSVRFSIIDSEWP
QVKANLQAKLQR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory