SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF1892 FitnessBrowser__psRCH2:GFF1892 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WID3 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; Phosphofructokinase B; Phosphohexokinase 2; Tagatose-6-phosphate kinase; EC 2.7.1.11; EC 2.7.1.144 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 98% coverage: 2:307/312 of query aligns to 12:315/339 of P9WID3

query
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P9WID3

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K283 (≠ E275) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

3uqdB Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
39% identity, 97% coverage: 1:304/312 of query aligns to 1:303/309 of 3uqdB

query
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3uqdB

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active site: G253 (= G254), A254 (= A255), G255 (= G256), D256 (= D257)
binding adenosine-5'-diphosphate: K185 (= K186), N187 (≠ S188), S224 (= S225), G226 (= G227), P227 (≠ A228), G229 (= G230), S248 (≠ V249), M258 (= M259), V280 (≠ I281), G283 (= G284), S284 (≠ A285)
binding adenosine-5'-triphosphate: Y23 (≠ I23), K27 (= K27)
binding 6-O-phosphono-beta-D-fructofuranose: K27 (= K27), R29 (= R29)
binding 1,6-di-O-phosphono-beta-D-fructofuranose: D14 (= D14), G39 (= G39), N43 (= N43), R90 (= R90), R105 (= R105), S139 (= S140), G253 (= G254)

3uqdA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with substrates and products (see paper)
39% identity, 97% coverage: 1:304/312 of query aligns to 1:303/309 of 3uqdA

query
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3uqdA

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active site: G253 (= G254), A254 (= A255), G255 (= G256), D256 (= D257)
binding adenosine-5'-triphosphate: K185 (= K186), S224 (= S225), G226 (= G227), P227 (≠ A228), G229 (= G230), T251 (≠ A252), G255 (= G256), M258 (= M259), V280 (≠ I281), G283 (= G284), S284 (≠ A285), T287 (≠ V288)
binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D14), G38 (= G38), G39 (= G39), N43 (= N43), R90 (= R90), S139 (= S140), D256 (= D257)
binding 1,6-di-O-phosphono-beta-D-fructofuranose: K27 (= K27), R29 (= R29)

3n1cA Crystal structure of the phosphofructokinase-2 from escherichia coli in complex with fructose-6-phosphate (see paper)
39% identity, 97% coverage: 1:304/312 of query aligns to 1:303/309 of 3n1cA

query
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3n1cA

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L

R

N

P

Q

G

G

T

T

E

R

L

L

C

C

H

S

R

H

E

D

D

D

V

T

Q

Q

R

K

L

I

active site: G253 (= G254), A254 (= A255), G255 (= G256), D256 (= D257)
binding 6-O-phosphono-beta-D-fructofuranose: D14 (= D14), K27 (= K27), R29 (= R29), G39 (= G39), N43 (= N43), R90 (= R90), G138 (= G139), S139 (= S140), D256 (= D257)

P06999 ATP-dependent 6-phosphofructokinase isozyme 2; ATP-PFK 2; Phosphofructokinase 2; 6-phosphofructokinase isozyme II; Phosphohexokinase 2; EC 2.7.1.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 97% coverage: 1:304/312 of query aligns to 1:303/309 of P06999

query
sites
P06999

M

M

P

V

L

R

I

I

A

Y

T

T

L

L

T

T

L

L

N

A

P

P

A

S

M

L

D

D

L

S

S

A

V

T

S

I

T

T

A

P

R

Q

V

I

I

Y

S

P

T

E

E

G

K
|
K

L

L

R

R

C

C

S

T

L

A

P

P

R

V

H

F

D

E

P

P

G

G

I

I

N

N

V

V

A

A

R

R

V

A

V

I

K

A

T

H

L

L

G

G

K

S

A

A

V

T

A

A

V

I

Y

F

P

P

A

A

G

G

P

A

F

T

G

G

D

E

L

H

L

L

Q

V

R

S

S

L

L

L

D

A

E

D

L

E

G

N

L

V

V

P

H

V

R

A

P

T

V

V

P

E

I

A

A

K

G

D

D

W

T

T

R

R

E

Q

S

N

F

L

T

H

V

V

D

H

E

V

L

E

A

A

S

S

G

G

L

E

Q

Q

Y

Y

R

R

F

F

V

V

L

M

P

P

G

G

P

A

T

A

L

L

S

N

A

E

Q

D

E

E

L

F

Q

R

Q

Q

C

L

L

E

D

E

S

Q

L

V

A

L

A

E

L

I

R

E

P

S

A

G

P

-

A

A

Y

I

V

L

V

V

L

I

S

S

G

G

S

S

F

L

P

P

G

G

A

V

D

K

L

L

G

E

F

K

F

L

D

T

E

Q

L

L

L

I

A

S

L

A

A

A

R

Q

R

K

I

Q

G

G

A

I

R

R

L

C

V

I

V

V

D

D

L

S

S

S

G

G

E

E

P

A

L

L

R

S

H

A

A

A

A

L

R

A

Q

I

G

G

G

N

V

I

Y

E

L

L

M

V

K
|
K

P
|
P

S
x
N

L
x
Q

D
x
K

E
|
E

L

L

G

S

T

A

L

L

M

V

G

N

R

R

T

E

V

L

S

T

A

Q

E

P

A

D

E

D

Q

V

E

R

Q

K

A

A

L

A

R

Q

S

E

L

I

I

V

R

N

Q

S

G

G

C

K

A

A

E

K

V

R

I

V

V

V

L

V

S
|
S

L
|
L

G
|
G

A
x
P

D
x
Q

G
|
G

A

A

L

L

Y

G

A

V

Y

D

G

S

D

E

Q

N

V

C

G

I

R

Q

L

V

R

V

T

P

P

P

E

P

V

V

P

K

V
x
S

A

Q

S
|
S

A

T

V
|
V

G

G

A

A

G

G

D

D

S

S

M

M

L

V

G

G

A

A

I

M

V

T

L

L

A

K

M

L

A

A

D

E

G

N

R

A

S

S

I

L

P

E

E

E

A

M

V

V

C

R

Q

F

G

G

I
x
V

A

A

G

G

A
x
S

A

A

T
x
A

V

T

M

L

R
x
N

P

Q

G
|
G

T

T

E
x
R

L

L

C

C

H

S

R

H

E

D

D

D

V

T

Q

Q

R

K

L

I

K27 (= K27) binding ; binding
KPN 185:187 (≠ KPS 186:188) binding in other chain
NQK 187:189 (≠ SLD 188:190) binding in other chain
E190 (= E191) mutation to Q: Causes a 50-fold decrease in the kcat value and a 15-fold increment in the apparent KM for ATP.
SLGPQG 224:229 (≠ SLGADG 225:230) binding in other chain
S248 (≠ V249) binding in other chain
S250 (= S251) binding
V252 (= V253) binding
V280 (≠ I281) binding in other chain
S284 (≠ A285) binding in other chain
A286 (≠ T287) binding
N289 (≠ R290) binding
G291 (= G292) binding
R293 (≠ E294) binding

3uqeA Crystal structure of the phosphofructokinase-2 mutant y23d from escherichia coli
38% identity, 97% coverage: 1:304/312 of query aligns to 1:301/307 of 3uqeA

query
sites
3uqeA

M

M

P

V

L

R

I

I

A

Y

T

T

L

L

T

T

L

L

N

A

P

P

A

S

M

L

D

D

L

S

S

A

V

T

S

I

T

T

A

P

R

Q

V

I

I

D

S

P

T

E

E

G

K

K

L

L

R

R

C

C

S

T

L

A

P

P

R

V

H

F

D

E

P

P

G

G

I

I

N

N

V

V

A

A

R

R

V

A

V

I

K

A

T

H

L

L

G

G

K

S

A

A

V

T

A

A

V

I

Y

F

P

P

A

A

G

G

P

A

F

T

G

G

D

E

L

H

L

L

Q

V

R

S

S

L

L

L

D

A

E

D

L

E

G

N

L

V

V

P

H

V

R

A

P

T

V

V

P

E

I

A

A

K

G

D

D

W

T

T

R

R

E

Q

S

N

F

L

T

H

V

V

D

H

E

V

L

E

A

A

S

S

G

G

L

E

Q

Q

Y

Y

R

R

F

F

V

V

L

M

P

P

G

G

P

A

T

A

L

L

S

N

A

E

Q

D

E

E

L

F

Q

R

Q

Q

C

L

L

E

D

E

S

Q

L

V

A

L

A

E

L

I

R

E

P

S

A

G

P

-

A

A

Y

I

V

L

V

V

L

I

S

S

G

G

S

S

F

L

P

P

G

G

A

V

D

K

L

L

G

E

F

K

F

L

D

T

E

Q

L

L

L

I

A

S

L

A

A

A

R

Q

R

K

I

Q

G

G

A

I

R

R

L

C

V

I

V

V

D

D

L

S

S

S

G

G

E

E

P

A

L

L

R

S

H

A

A

A

A

L

R

A

Q

I

G

G

G

N

V

I

Y

E

L

L

M

V

K
|
K

P

P

S
x
N

L

Q

D
x
K

E

E

L

L

G

S

T

A

L

L

M

V

G

N

R

R

T

E

V

L

S

T

A

Q

E

P

A

D

E

D

Q

V

E

R

Q

K

A

A

L

A

R

Q

S

E

L

I

I

V

R

N

Q

S

G

G

C

K

A

A

E

K

V

R

I

V

V

V

L

V

S
|
S

L

L

G
|
G

A
x
P

D

Q

G
|
G

A

A

L

L

Y

G

A

V

Y

D

G

S

D

E

Q

N

V

C

G

I

R

Q

L

V

R

V

T

P

P

P

E

P

V

V

P

K

V
x
S

A

Q

S

S

A

T

V

V

G
|
G

A
|
A

G
|
G

D
|
D

S

S

M
|
M

L

V

G

G

A

A

I

M

V

T

L

L

A

K

M

L

A

A

D

E

G

N

R

A

S

S

I

L

P

E

E

E

A

M

V

V

C

R

Q

F

G

G

I
x
V

A

A

G
|
G

A
x
S

A

A

T

A

V
x
T

M

L

R

N

P

-

G

-

T

Q

E

R

L

L

C

C

H

S

R

H

E

D

D

D

V

T

Q

Q

R

K

L

I

active site: G253 (= G254), A254 (= A255), G255 (= G256), D256 (= D257)
binding adenosine-5'-triphosphate: K185 (= K186), N187 (≠ S188), S224 (= S225), G226 (= G227), P227 (≠ A228), G229 (= G230), S248 (≠ V249), A254 (= A255), G255 (= G256), M258 (= M259), V280 (≠ I281), G283 (= G284), S284 (≠ A285), T287 (≠ V288)
binding pyrophosphate 2-: N187 (≠ S188), K189 (≠ D190)

3cqdA Structure of the tetrameric inhibited form of phosphofructokinase-2 from escherichia coli (see paper)
38% identity, 97% coverage: 1:304/312 of query aligns to 1:298/304 of 3cqdA

query
sites
3cqdA

M

M

P

V

L

R

I

I

A

Y

T

T

L

L

T

T

L

L

N

A

P

P

A

S

M

L

D

D

L

S

S

A

V

T

S

I

T

T

A

P

R

Q

V

I

I
x
Y

S

P

T

E

E
x
G

K
|
K

L

L

R

R

C

C

S

T

L

A

P

P

R

V

H

F

D

E

P

P

G

G

I

I

N

N

V

V

A

A

R

R

V

A

V

I

K

A

T

H

L

L

G

G

K

S

A

A

V

T

A

A

V

I

Y

F

P

P

A

A

G

G

P

A

F

T

G

G

D

E

L

H

L

L

Q

V

R

S

S

L

L

L

D

A

E

D

L

E

G

N

L

V

V

P

H

V

R

A

P

T

V

V

P

E

I

A

A

K

G

D

D

W

T

T

R

R

E

Q

S

N

F

L

T

H

V

V

D

H

E

V

L

E

A

A

S

S

G

G

L

E

Q

Q

Y

Y

R

R

F

F

V

V

L

M

P

P

G

G

P

A

T

A

L

L

S

N

A

E

Q

D

E

E

L

F

Q

R

Q

Q

C

L

L

E

D

E

S

Q

L

V

A

L

A

E

L

I

R

E

P

S

A

G

P

-

A

A

Y

I

V

L

V

V

L

I

S

S

G

G

S

S

F

L

P

P

G

G

A

V

D

K

L

L

G

E

F

K

F

L

D

T

E

Q

L

L

L

I

A

S

L

A

A

A

R

Q

R

K

I

Q

G

G

A

I

R

R

L

C

V

I

V

V

D

D

L

S

S

S

G

G

E

E

P

A

L

L

R

S

H

A

A

A

A

L

R

A

Q

I

G

G

G

N

V

I

Y

E

L

L

M

V

K
|
K

P

P

S
x
N

L

Q

D
x
K

E

E

L

L

G

S

T

A

L

L

M

V

G

N

R

R

T

E

V

L

S

T

A

Q

E

P

A

D

E

D

Q

V

E

R

Q

K

A

A

L

A

R

Q

S

E

L

I

I

V

R

N

Q

S

G

G

C

K

A

A

E

K

V

R

I

V

V

V

L

V

S
|
S

L

L

G
|
G

A
x
P

D

Q

G
|
G

A

A

L

L

Y

G

A

V

Y

D

G

S

D

E

Q

N

V

C

G

I

R

Q

L

V

R

V

T

P

P

P

E

P

V

V

P

K

V
x
S

A

Q

S

S

A
x
T

V

V

G
|
G

A
|
A

G
|
G

D
|
D

S

S

M
|
M

L

V

G

G

A

A

I

M

V

T

L

L

A

K

M

L

A

A

D

E

G

N

R

A

S

S

I

L

P

E

E

E

A

M

V

V

C

R

Q

F

G

G

I
x
V

A

A

G
|
G

A
x
S

A

A

T

A

V
x
T

M

L

R

-

P

-

G

-

T

-

E

-

L

L

C

C

H

S

R

H

E

D

D

D

V

T

Q

Q

R

K

L

I

active site: G253 (= G254), A254 (= A255), G255 (= G256), D256 (= D257)
binding adenosine-5'-triphosphate: Y23 (≠ I23), G26 (≠ E26), K27 (= K27), K185 (= K186), N187 (≠ S188), N187 (≠ S188), K189 (≠ D190), S224 (= S225), G226 (= G227), P227 (≠ A228), G229 (= G230), S248 (≠ V249), T251 (≠ A252), A254 (= A255), G255 (= G256), M258 (= M259), V280 (≠ I281), G283 (= G284), S284 (≠ A285), T287 (≠ V288)

2f02A Crystal structure of lacc from enterococcus faecalis in complex with atp
32% identity, 97% coverage: 3:305/312 of query aligns to 2:304/319 of 2f02A

query
sites
2f02A

L

L

I

I

A

V

T

T

L

V

T

T

L

M

N

N

P

P

A

S

M

I

D

D

L

I

S

S

V

Y

S

L

T

L

A

D

R

H

V

L

I

K

S

L

T

D

E

T

K

V

L

N

R

R

C

T

S

S

L

Q

P

V

R

T

H

K

D

T

P

P

G

G

G

K

G

G

I

L

N

N

V

V

A

T

R

R

V

V

V

I

K

H

T

D

L

L

G

G

K

D

A

V

V

I

A

A

V

T

Y

G

P

V

A

L

G

G

P

F

F

H

G

G

D

A

L

F

L

I

Q

A

R

N

S

E

L

L

D

K

E

K

L

A

G

N

L

I

V

P

H

Q

R

A

P

F

V

T

P

S

I

I

A

K

G

E

D

E

T

T

R

R

E

D

S

S

F

I

T

A

V

I

D

-

E

-

L

L

A

H

S

E

G

G

L

N

Q

Q

Y

T

R

E

F

I

V

L

L

E

P

A

G

G

P

P

T

T

L

V

S

S

A

P

Q

E

E

E

L

I

Q

S

Q

N

C

F

L

L

D

E

S

N

L

F

A

D

A

Q

L

L

R

I

P

K

A

Q

P

A

A

E

Y

I

V

V

V

T

L

I

S

S

G

G

S

S

F

L

P

A

P

K

G

G

A

L

D

P

L

S

G

D

F

F

F

Y

D

Q

E

E

L

L

L

V

A

Q

L

K

A

A

R

H

R

A

I

Q

G

E

A

V

R

K

L

V

V

L

D

D

L

T

S

S

G

G

E

D

P

S

L

L

R

R

H

Q

A

V

A

L

R

-

Q

Q

G

G

G

P

V

W

-

K

-

P

Y

Y

L

L

M

I

K

K

P

P

S
x
N

L

L

D

E

E

E

L

L

G

E

T

G

L

L

M

L

G

G

R

Q

T

D

V

F

S

S

A

E

E

N

-

P

-

L

A

A

E

A

Q

V

E

Q

Q

T

A

A

L

L

R

T

S

K

L

P

I

M

R

F

Q

A

G

G

C

I

A

-

E

E

V

W

I

I

V

V

L

I

S
|
S

L

L

G
|
G

A

K

D

D

G

G

A

A

L

I

Y

A

A

K

Y

H

G

H

D

D

Q

Q

V

F

G

Y

R

R

L

V

R

K

T
x
I

P

P

E

T

V
x
I

P

Q

V

A

A

K

S

N

A

P

V

V

G
|
G

A
x
S

G
|
G

D

D

S

A

M
x
T

L

I

G

A

A

G

I

L

V

A

L

Y

A

G

M

L

A

A

D

K

G

D

R

A

S

P

I

A

P

A

E

E

A

L

V

L

C

K

Q

W

G

G

I
x
M

A

A

G
|
G

A
x
M

A

A

T

N

V

A

M

Q

R

E

P

R

G

M

T

T

E

G

L

H

C

V

H

D

R

V

E

E

D

N

V

V

Q

K

R

K

L

H

L

L

binding adenosine-5'-triphosphate: N186 (≠ S188), S224 (= S225), G226 (= G227), I243 (≠ T244), I246 (≠ V247), G253 (= G254), S254 (≠ A255), G255 (= G256), T258 (≠ M259), M280 (≠ I281), G283 (= G284), M284 (≠ A285)

2jgvB Structure of staphylococcus aureus d-tagatose-6-phosphate kinase in complex with adp (see paper)
28% identity, 87% coverage: 3:272/312 of query aligns to 5:273/314 of 2jgvB

query
sites
2jgvB

L

M

I

I

A

L

T

T

L

L

T

T

L

L

N

N

P

P

A

S

M

V

D

D

L

I

S

S

V

Y

S

P

T

L

A

T

R

A

V

L

I

K

S

L

T

D

E

D

K

V

L

N

R

R

C

V

S

Q

L

E

P

V

R

S

H

K

D

T

P

A

G

G

G

K

G

G

I

L

N

N

V

V

A

T

R

R

V

V

V

L

K

A

T

Q

L

V

G

G

G

E

K

P

A

V

V

L

A

A

V

S

Y

G

P

F

A

I

G

G

P

E

F

L

G

G

D

Q

L

F

L

I

Q

A

R

K

S

K

L

L

D

D

E

H

L

A

G

D

L

I

V

K

H

H

R

A

P

F

V

Y

P

N

I

I

A

K

G

G

D

E

T

T

R

R

E

N

S

C

F

I

T

A

V

I

D

-

E

-

L

L

A

H

S

E

G

G

L

Q

Q

Q

Y

T

R

E

F

I

V

L

L

E

P

Q

G

G

P

P

T

E

L

I

S

D

A

N

Q

Q

E

E

L

A

Q

A

Q

G

C

F

L

I

D

K

S

H

L

F

A

E

A

Q

L

L

R

L

P

E

A

K

P

V

A

E

Y

A

V

V

V

A

L

I

S

S

G

G

S

S

F

L

P

P

P

K

G

G

A

L

D

N

L

Q

G

D

F

Y

D

A

E

Q

L

I

A

E

L

R

A

C

R

Q

R

N

I

K

G

G

A

V

R

P

L

V

V

I

V

L

D

D

L

C

S

S

G

G

E

A

P

T

L

L

R

Q

H

T

A

V

A

L

R

E

Q

N

-

P

G

Y

G

K

V

P

Y

T

L

V

M

I

K

K

P

P

S

N

L

I

D

S

E

E

L

L

G

Y

T

Q

L

L

M

L

G

N

R

Q

T

P

V

L

S

D

A

E

E

S

A

L

E

E

Q

S

-

L

E

K

Q

Q

A

A

L

V

R

S

S

Q

L

P

I

L

R

F

Q

E

G

G

C

I

A

-

E

E

V

W

I

I

V

I

L

V

S
|
S

L

L

G

G

A
|
A

D

Q

G

G

A

A

L

F

Y

A

A

K

Y

H

G

N

D

H

Q

T

V

F

G

Y

R

R

L

V

R

N

T

I

P

P

E

T

V
x
I

P

S

V

V

A

L

S

N

A
x
P

V

V

G
|
G

A
x
S

G
|
G

D
|
D

S

S

M

T

L

V

G

A

A

G

I

I

V

T

L

S

A

A

M

I

A

L

D

N

G

H

R

E

S

N

active site: G255 (= G254), S256 (≠ A255), G257 (= G256), D258 (= D257)
binding adenosine-5'-diphosphate: S226 (= S225), A229 (= A228), I248 (≠ V247), P253 (≠ A252), S256 (≠ A255), G257 (= G256)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 282, 285, 286

2jg1C Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
27% identity, 87% coverage: 3:272/312 of query aligns to 6:274/315 of 2jg1C

query
sites
2jg1C

L

M

I

I

A

L

T

T

L

L

T

T

L

L

N

N

P

P

A

S

M

V

D
|
D

L

I

S

S

V

Y

S

P

T

L

A

T

R

A

V

L

I

K

S

L

T

D

E

D

K

V

L

N

R

R

C

V

S

Q

L

E

P

V

R

S

H

K

D

T

P

A

G

G

G
|
G

G
x
K

G

G

I

L

N

N

V

V

A

T

R

R

V

V

V

L

K

A

T

Q

L

V

G

G

G

E

K

P

A

V

V

L

A

A

V

S

Y

G

P

F

A

I

G

G

P

E

F

L

G

G

D

Q

L

F

L

I

Q

A

R

K

S

K

L

L

D

D

E

H

L

A

G

D

L

I

V

K

H

H

R

A

P

F

V

Y

P

N

I

I

A

K

G

G

D

E

T

T

R
|
R

E

N

S
x
C

F

I

T

A

V

I

D

-

E

-

L

L

A

H

S

E

G

G

L

Q

Q

Q

Y

T

R

E

F

I

V
x
L

L

E

P

Q

G

G

P

P

T

E

L

I

S

D

A

N

Q

Q

E

E

L

A

Q

A

Q

G

C

F

L

I

D

K

S

H

L

F

A

E

A

Q

L

M

R

M

P

E

A

K

P

V

A

E

Y

A

V

V

V

A

L

I

S

S

G
|
G

S
|
S

F

L

P

P

P

K

G

G

A

L

D

N

L

Q

G

D

F

Y

D

A

E

Q

L

I

A

E

L

R

A

C

R

Q

R

N

I

K

G

G

A

V

R

P

L

V

V

I

V

L

D

D

L

C

S

S

G

G

E

A

P

T

L

L

R

Q

H

T

A

V

A

L

R

E

Q

N

-

P

G

Y

G

K

V

P

Y

T

L

V

M

I

K

K

P

P

S

N

L

I

D

S

E

E

L

L

G

Y

T

Q

L

L

M

L

G

N

R

Q

T

P

V

L

S

D

A

E

E

S

A

L

E

E

Q

S

-

L

E

K

Q

Q

A

A

L

V

R

S

S

Q

L

P

I

L

R

F

Q

E

G

G

C

I

A

-

E

E

V

W

I

I

V

I

L

V

S
|
S

L

L

G
|
G

A
|
A

D

Q

G
|
G

A

A

L

F

Y

A

A

K

Y

H

G

N

D

H

Q

T

V

F

G

Y

R

R

L

V

R

N

T
x
I

P

P

E

T

V
x
I

P

S

V
|
V

A

L

S

N

A

P

V
|
V

G
|
G

A
x
S

G
|
G

D
|
D

S

S

M
x
T

L

V

G

A

A

G

I

I

V

T

L

S

A

A

M

I

A

L

D

N

G

H

R

E

S

N

active site: G256 (= G254), S257 (≠ A255), G258 (= G256), D259 (= D257)
binding phosphoaminophosphonic acid-adenylate ester: S227 (= S225), G229 (= G227), A230 (= A228), G232 (= G230), I246 (≠ T244), I249 (≠ V247), V251 (= V249), V255 (= V253), G256 (= G254), S257 (≠ A255), G258 (= G256), D259 (= D257), T261 (≠ M259)
binding 6-O-phosphono-beta-D-tagatofuranose: D17 (= D14), G42 (= G39), K43 (≠ G40), R93 (= R90), C95 (≠ S92), L108 (≠ V107), G140 (= G139), S141 (= S140), D259 (= D257)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 283, 286, 287

2jg1A Structure of staphylococcus aureus d-tagatose-6-phosphate kinase with cofactor and substrate (see paper)
27% identity, 87% coverage: 3:272/312 of query aligns to 9:277/318 of 2jg1A

query
sites
2jg1A

L

M

I

I

A

L

T

T

L

L

T

T

L

L

N

N

P

P

A

S

M

V

D

D

L

I

S

S

V

Y

S

P

T

L

A

T

R

A

V

L

I

K

S

L

T

D

E

D

K

V

L

N

R

R

C

V

S

Q

L

E

P

V

R

S

H

K

D

T

P

A

G

G

G
x
K

G

G

I

L

N

N

V

V

A

T

R

R

V

V

V

L

K

A

T

Q

L

V

G

G

G

E

K

P

A

V

V

L

A

A

V

S

Y

G

P

F

A

I

G

G

P

E

F

L

G

G

D

Q

L

F

L

I

Q

A

R

K

S

K

L

L

D

D

E

H

L

A

G

D

L

I

V

K

H

H

R

A

P

F

V

Y

P

N

I

I

A

K

G

G

D

E

T

T

R

R

E

N

S

C

F

I

T

A

V

I

D

-

E

-

L

L

A

H

S

E

G

G

L

Q

Q

Q

Y

T

R

E

F

I

V

L

L

E

P

Q

G

G

P

P

T

E

L

I

S

D

A

N

Q

Q

E

E

L

A

Q

A

Q

G

C

F

L

I

D

K

S

H

L

F

A

E

A

Q

L

M

R

M

P

E

A

K

P

V

A

E

Y

A

V

V

V

A

L

I

S

S

G

G

S

S

F

L

P

P

P

K

G

G

A

L

D

N

L

Q

G

D

F

Y

D

A

E

Q

L

I

A

E

L

R

A

C

R

Q

R

N

I

K

G

G

A

V

R

P

L

V

V

I

V

L

D

D

L

C

S

S

G

G

E

A

P

T

L

L

R

Q

H

T

A

V

A

L

R

E

Q

N

-

P

G

Y

G

K

V

P

Y

T

L

V

M

I

K

K

P

P

S
x
N

L

I

D

S

E

E

L

L

G

Y

T

Q

L

L

M

L

G

N

R

Q

T

P

V

L

S

D

A

E

E

S

A

L

E

E

Q

S

-

L

E

K

Q

Q

A

A

L

V

R

S

S

Q

L

P

I

L

R

F

Q

E

G

G

C

I

A

-

E

E

V

W

I

I

V

I

L

V

S
|
S

L

L

G
|
G

A

A

D

Q

G
|
G

A

A

L

F

Y

A

A

K

Y

H

G

N

D

H

Q

T

V

F

G

Y

R

R

L

V

R

N

T

I

P

P

E

T

V
x
I

P

S

V
|
V

A

L

S

N

A

P

V

V

G
|
G

A
x
S

G
|
G

D
|
D

S

S

M
x
T

L

V

G

A

A

G

I

I

V

T

L

S

A

A

M

I

A

L

D

N

G

H

R

E

S

N

active site: G259 (= G254), S260 (≠ A255), G261 (= G256), D262 (= D257)
binding phosphoaminophosphonic acid-adenylate ester: K46 (≠ G40), N193 (≠ S188), S230 (= S225), G232 (= G227), G235 (= G230), I252 (≠ V247), V254 (= V249), G259 (= G254), S260 (≠ A255), G261 (= G256), D262 (= D257), T264 (≠ M259)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 286, 289, 290

3ie7A The crystal structure of phosphofructokinase (lin2199) from listeria innocua in complex with atp at 1.6a
32% identity, 92% coverage: 3:290/312 of query aligns to 2:285/309 of 3ie7A

query
sites
3ie7A

L

L

I

I

A

Y

T

T

L

I

T

T

L

L

N

N

P

P

A

A

M

I

D

D

L

R

S

L

V

L

-

F

-

I

-

R

-

G

-

E

-

L

-

E

-

K

-

R

S

K

T

T

A

N

R

R

V

V

I

I

S

K

T

T

E

E

K

-

L

-

R

-

C

-

S

-

L

-

P

-

R

-

H

F

D

D

P

C

G

G

G

K

G

G

I

L

N

H

V

V

A

S

R

G

V

V

V

L

K

S

T

K

L

F

G

G

G

I

K

K

A

N

V

E

A

A

V

L

Y

G

P

I

A

A

G

G

G

S

P

D

F

N

G

L

D

D

L

K

L

L

Q

Y

R

A

S

I

L

L

D

K

E

E

L

K

G

H

L

I

V

N

H

H

R

D

P

F

V

L

P

V

I

E

A

A

G

G

-

T

D

S

T

T

R

R

E

E

S

C

F

F

T

V

V

V

D

-

E

-

L

L

A

S

S

D

G

D

L

T

Q

N

Y

G

R

S

F

T

V

M

L

I

P

P

-

E

-

A

G

G

P

F

T

T

L

V

S

S

A

Q

Q

T

E

N

L

K

Q

D

Q

N

C

L

L

L

D

K

S

Q

L

I

A

A

A

K

L

K

R

V

P

K

A

K

P

E

A

D

Y

M

V

V

L

I

S

A

G

G

S

S

F

P

P

P

G

H

A

Y

D

T

L

L

G

S

F

D

F

F

D

K

E

E

L

L

A

R

L

T

A

V

R

K

R

A

I

T

G

G

A

A

R

F

L

L

V

G

V

C

D

D

L

N

S

S

G

G

E

E

P

Y

L

L

R

N

H

L

A

A

A

V

R

E

Q

M

G

G

G

-

V

V

Y

D

L

F

M

I

K

K

P

P

S
x
N

L

E

D

D

E

E

L

V

G

I

T

A

L

I

M

L

G

D

R

E

T

K

V

T

S

N

A

S

E

-

A

-

E

-

Q

L

E

E

Q

E

A

N

L

I

R

R

S

T

L

L

I

A

R

E

Q

K

G

-

C

-

A

I

E

P

V

Y

I

L

V

V

L

V

S
|
S

L

L

G
|
G

A
|
A

D

K

G
|
G

A

S

L

I

Y

C

A

A

Y

H

G

N

D

G

Q

K

V

L

G

Y

R

Q

L

V

R

I

T

P

P

P

E

K

V
|
V

P

Q

V

E

A

R

S

N

A

D

V

T

G
|
G

A
|
A

G
|
G

D
|
D

S

V

M

F

L

V

G

G

A

A

I

F

V

I

L

A

A

G

M

L

A

A

D

M

G

N

R

M

S

P

I

I

P

T

E

E

A

T

V

L

C

K

Q

V

G

A

I

T

A

G

A

C

G
x
S

A

A

A

S

T

K

V
|
V

M

M

R

Q

binding adenosine-5'-triphosphate: N188 (≠ S188), S220 (= S225), G222 (= G227), A223 (= A228), G225 (= G230), V242 (= V247), G249 (= G254), A250 (= A255), G251 (= G256), D252 (= D257), S279 (≠ G284), V283 (= V288)

3julA Crystal structure of listeria innocua d-tagatose-6-phosphate kinase bound with substrate
31% identity, 92% coverage: 3:290/312 of query aligns to 2:278/298 of 3julA

query
sites
3julA

L

L

I

I

A

Y

T

T

L

I

T

T

L

L

N

N

P

P

A

A

M

I

D
|
D

L

R

S

L

V

L

-

F

-

I

-

R

-

G

-

E

-

L

-

E

-

K

-

R

S

K

T

T

A

N

R

R

V

V

I

I

S

K

T

T

E

E

K

-

L

-

R

-

C

-

S

-

L

-

P

-

R

-

H

F

D

D

P

C

G

G

G
x
K

G

G

I

L

N
x
H

V

V

A

S

R

G

V

V

V

L

K

S

T

K

L

F

G

G

G

I

K

K

A

N

V

E

A

A

V

L

Y

G

P

I

A

A

G

G

G

S

P

D

F

N

G

L

D

D

L

K

L

L

Q

Y

R

A

S

I

L

L

D

K

E

E

L

K

G

H

L

I

V

N

H

H

R

D

P

F

V

L

P

V

I

E

A

A

G

G

-

T

D

S

T

T

R
|
R

E

E

S

C

F

F

T

V

V

V

D

-

E

-

L

L

A

S

S

D

G

D

L

T

Q

N

Y

G

R

S

F

T

V
x
M

L

I

P

P

-

E

-

A

G

G

P

F

T

T

L

V

S

S

A

Q

Q

T

E

N

L

K

Q

D

Q

N

C

L

L

L

D

K

S

Q

L

I

A

A

A

K

L

K

R

V

P

K

A

K

P

E

A

D

Y

M

V

V

L

I

S

A

G
|
G

S

S

F

P

P

P

G

H

A

Y

D

T

L

L

G

S

F

D

F

F

D

K

E

E

L

L

A

R

L

T

A

V

R

K

R

A

I

T

G

G

A

A

R

F

L

L

V

G

V

C

D

D

L

N

S

S

G

G

E

E

P

Y

L

L

R

N

H

L

A

A

A

V

R

E

Q

M

G

-

G

G

V

V

Y

D

L

F

M

I

K

K

P

P

S

N

L

E

D

D

E

E

L

V

G

I

T

A

L

I

M

L

G

D

R

E

T

K

V

T

S

N

A

S

E

-

A

-

E

-

Q

L

E

E

Q

E

A

N

L

I

R

R

S

T

L

L

I

A

R

E

Q

K

G

-

C

-

A

I

E

P

V

Y

I

L

V

V

L

V

S

S

L

L

G

G

A

A

D

K

G

G

A

S

L

I

Y

C

A

A

Y

H

G

N

D

G

Q

K

V

L

G

Y

R

Q

L

V

R

I

T

P

P

P

E

-

V

-

P

-

V

-

A

-

S

-

A

-

V

T

G

G

A

A

G

G

D
|
D

S

V

M

F

L

V

G

G

A

A

I

F

V

I

L

A

A

G

M

L

A

A

D

M

G

N

R

M

S

P

I

I

P

T

E

E

A

T

V

L

C

K

Q

V

G

A

I

T

A

G

A

C

G

S

A

A

A

S

T

K

V

V

M

M

R

Q

binding 6-O-phosphono-beta-D-tagatofuranose: D13 (= D14), K40 (≠ G40), H43 (≠ N43), R91 (= R90), M106 (≠ V107), G140 (= G139), D245 (= D257)

2ajrA Crystal structure of possible 1-phosphofructokinase (ec 2.7.1.56) (tm0828) from thermotoga maritima at 2.46 a resolution
24% identity, 96% coverage: 3:303/312 of query aligns to 2:309/320 of 2ajrA

query
sites
2ajrA

L

M

I

V

A

L

T

T

L

V

T

T

L

L

N

N

P

P

A

A

M

L

D

D

L

R

S

E

V

I

S

F

T

I

A

E

R

D

V

F

I

Q

S

V

T

N

E

R

K

L

L

Y

R

R

C

I

-

N

-

D

-

L

S

S

L

K

P

T

R

Q

H

M

D

S

P

P

G

G

G

K

G

G

I

I

N

N

V

V

A

S

R

I

V

A

V

L

K

S

T

K

L

L

G

G

G

V

K

P

A

S

V

V

A

A

V

T

Y

G

P

F

A

V

G

G

P

Y

F

M

G

G

D

K

L

I

L

L

Q

V

R

E

S

E

L

L

D

R

E

K

L

I

G

S

-

K

-

L

L

I

V

T

H

T

R

N

P

F

V

V

P

Y

I

V

A

E

G

G

D

E

T

T

R

R

E

E

S

N

F

I

T

E

V

I

D

I

E

D

L

E

A

K

S

N

G

K

L

T

Q

I

Y

T

R

A

F

I

V

N

L

F

P

P

G

G

P

P

T

D

L

V

S

T

A

D

Q

M

E

D

L

V

Q

N

Q

H

C

F

L

L

D

R

S

R

L

Y

A

K

A

M

L

T

R

L

P

S

A

K

P

V

A

D

Y

C

V

V

L

I

S

S

G

G

S

S

F

I

P

P

G

G

A

V

D

N

L

E

G

G

F

I

F

C

D

N

E

E

L

L

L

V

A

R

L

L

A

A

R

R

R

E

I

R

G

G

A

V

R

F

L

V

V

F

V

V

D

E

L

Q

S

T

G

P

E

R

P

L

L

L

R

E

H

R

A

I

A

Y

R

-

Q

E

G

G

G

P

V

E

Y

F

-

P

-

N

L

V

M

V

K

K

P

P

S

D

L

L

D

R

-

G

E

N

L

H

G

A

T

S

L

F

M

L

G

G

R

V

T

D

V

L

S

K

A

T

E

F

A

D

E

D

Q

Y

E

V

Q

K

A

L

L

A

R

E

S

K

L

L

I

A

R

E

Q

K

G

-

C

-

A

S

E

Q

V

V

I

S

V

V

L

V

S

S

L

Y

G

E

A

V

D

K

G

N

A

D

L

I

Y

V

A

A

Y

T

G

R

D

E

Q

G

V

V

G

W

R

L

L

I

R

R

T

S

P

K

-

E

E

E

V

I

P

D

V

T

A

S

S
x
H

A

L

V

L

G

G

A

A

G

G

D

D

S

A

M

Y

L

V

-

A

G

G

A

M

I

V

V

Y

L

Y

A

F

M

I

A

K

D

H

G

G

R

A

S

N

I

F

P

L

E

E

A

M

V

A

C

K

Q

F

G

G

I

F

A

A

A

S

G

A

A

L

A

A

T

A

V

T

M

R

R
|
R

P

K

G
x
E

T

K

E

Y

L

M

C

P

H

D

R

L

E

E

D

A

V

I

Q

K

R

K

binding magnesium ion: H256 (≠ S251), R296 (= R290), E298 (≠ G292)

Q97U29 2-dehydro-3-deoxygluconokinase/2-dehydro-3-deoxygalactonokinase; 2-dehydro-3-deoxyglucono/galactono-kinase; 2-keto-3-deoxy-galactonokinase; 2-keto-3-deoxygluconokinase; 3-deoxy-2-oxo-D-gluconate kinase; KDG kinase; KDGal kinase; EC 2.7.1.178 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
33% identity, 33% coverage: 207:310/312 of query aligns to 209:313/313 of Q97U29

query
sites
Q97U29

E

D

Q

E

A

A

L

Y

R

R

S

K

L

Y

I

K

R

E

Q

L

G

G

C

V

A

-

E

K

V

V

I

L

V

L

L

Y

S
x
K

L
|
L

G
|
G

A
x
S

D
x
K

G
|
G

A

A

L

I

Y

-

A

A

Y

Y

G

K

D

D

Q

N

V

V

G

K

R

A

L

F

R

K

T

D

P

A

-

Y

E

K

V

V

P

P

V

V

A

E

S

D

A

P

V

T

G
|
G

A
|
A

G
|
G

D
|
D

S

A

M

M

L

A

G

G

A

T

I

F

V

V

L

S

A

L

M

Y

A

L

D

Q

G

G

R

K

S

D

I

I

P

E

E

Y

A

S

V

L

C

A

Q

H

G

G

I

I

A

A

G

S

A

T

A

L

T

V

V

I

-

T

M

V

R

R

P

G

G
x
D

T

N

E

E

L

L

C

T

-

P

H

T

R

L

E

E

D

D

V

A

Q

E

R

R

L

F

L

L

Q

N

L

E

M

F

E

K

S

T

KLGSKG 226:231 (≠ SLGADG 225:230) binding
GAGD 255:258 (= GAGD 254:257) binding
D258 (= D257) binding
D294 (≠ G292) binding

Sites not aligning to the query:

34:38 binding
90 binding
106:108 binding
164:166 binding
166 binding

2varA Crystal structure of sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate (see paper)
34% identity, 32% coverage: 207:306/312 of query aligns to 208:308/311 of 2varA

query
sites
2varA

E

D

Q

E

A

A

L

Y

R

R

S

K

L

Y

I

K

R

E

Q

L

G

G

C

V

A

-

E

K

V

V

I

L

V

L

L

Y

S
x
K

L

L

G
|
G

A
x
S

D

K

G
|
G

A

A

L

I

Y

-

A

A

Y

Y

G

K

D

D

Q

N

V

V

G

K

R

A

L

F

R

K

T

D

P

A

-

Y

E

K

V

V

P

P

V

V

A

E

S

D

A

P

V
x
T

G
|
G

A
|
A

G
|
G

D
|
D

S

A

M
|
M

L

A

G

G

A

T

I

F

V

V

L

S

A

L

M

Y

A

L

D

Q

G

G

R

K

S

D

I

I

P

E

E

Y

A

S

V

L

C

A

Q

H

G

G

I
|
I

A

A

G
x
S

A

T

A

L

T

V

V
x
I

-

T

M

V

R

R

P

G

G
x
D

T

N

E

E

L

L

C

T

-

P

H

T

R

L

E

E

D

D

V

A

Q

E

R

R

L

F

L

L

Q

N

active site: G254 (= G254), A255 (= A255), G256 (= G256), D257 (= D257)
binding adenosine monophosphate: G227 (= G227), G230 (= G230), M259 (= M259), S284 (≠ G284), I288 (≠ V288)
binding phosphoaminophosphonic acid-adenylate ester: K225 (≠ S225), G227 (= G227), S228 (≠ A228), G230 (= G230), G254 (= G254), A255 (= A255), G256 (= G256), D257 (= D257), M259 (= M259), I281 (= I281), S284 (≠ G284), I288 (≠ V288)
binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: G254 (= G254), D257 (= D257)
binding 2-keto-3-deoxygluconate: T253 (≠ V253), G254 (= G254), D257 (= D257), D293 (≠ G292)

Sites not aligning to the query:

binding 3-deoxy-alpha-D-erythro-hex-2-ulofuranosonic acid: 10, 33, 34, 89, 105, 107, 136, 165
binding 2-keto-3-deoxygluconate: 33, 34, 89, 103, 105, 107, 136, 165

1tz3A Crystal structure of aminoimidazole riboside kinase complexed with aminoimidazole riboside (see paper)
31% identity, 35% coverage: 184:292/312 of query aligns to 174:281/299 of 1tz3A

query
sites
1tz3A

L

I

M

C

K

K

P

V

S

S

L

A

D

D

E

E

L

L

G

C

T

Q

L

L

M

S

G

G

R

-

T

-

V

-

S

-

A

-

E

-

A

A

E

S

Q

H

E

W

Q

Q

A

D

L

A

R

R

S

Y

L

Y

I

L

R

R

Q

D

G

L

C

G

A

C

E

D

V

T

I

T

V

I

L

I

S

S

L

L

G

G

A

A

D

D

G

G

A

A

L

L

Y

L

A

I

Y

T

G

A

D

E

Q

G

V

E

G

F

R

H

L

F

R

P

T

A

P

P

E

R

V

V

P

D

V

V

A

V

S

D

A

T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

S

A

M

F

L

V

G

G

A

G

I

L

V

L

L

F

A

T

M

L

A

S

-

R

-

A

-

N

-

C

-

W

D

D

G

H

R

A

S

L

I

L

P

A

E

E

A

A

V

I

C

S

Q

N

G

A

I

N

A

A

A

C

G

G

A

A

A

M

T

A

V

V

M

T

R

A

P

K

G

G

active site: G238 (= G254), A239 (= A255), G240 (= G256), D241 (= D257)
binding 5-aminoimidazole ribonucleoside: D241 (= D257)

Sites not aligning to the query:

active site: 24, 88
binding 5-aminoimidazole ribonucleoside: 8, 10, 12, 27, 83, 88, 90, 151, 154

1tz6A Crystal structure of aminoimidazole riboside kinase from salmonella enterica complexed with aminoimidazole riboside and atp analog (see paper)
31% identity, 35% coverage: 184:292/312 of query aligns to 174:281/297 of 1tz6A

query
sites
1tz6A

L

I

M

C

K
|
K

P

V

S

S

L

A

D

D

E
|
E

L

L

G

C

T

Q

L

L

M

S

G

G

R

-

T

-

V

-

S

-

A

-

E

-

A

A

E

S

Q

H

E

W

Q

Q

A

D

L

A

R

R

S

Y

L

Y

I

L

R

R

Q

D

G

L

C

G

A

C

E

D

V

T

I

T

V

I

L

I

S
|
S

L

L

G
|
G

A
|
A

D

D

G
|
G

A

A

L

L

Y

L

A

I

Y

T

G

A

D

E

Q

G

V

E

G

F

R

H

L

F

R

P

T

A

P

P

E

R

V

V

P

D

V

V

A

V

S

D

A

T

V

T

G
|
G

A
|
A

G
|
G

D
|
D

S

A

M
x
F

L

V

G

G

A

G

I

L

V

L

L

F

A

T

M

L

A

S

-

R

-

A

-

N

-

C

-

W

D

D

G

H

R

A

S

L

I

L

P

A

E

E

A

A

V

I

C

S

Q

N

G

A

I
x
N

A

A

A

C

G
|
G

A
|
A

A

M

T

A

V

V

M

T

R

A

P

K

G

G

active site: G238 (= G254), A239 (= A255), G240 (= G256), D241 (= D257)
binding phosphomethylphosphonic acid adenylate ester: K176 (= K186), E181 (= E191), S209 (= S225), G211 (= G227), A212 (= A228), G214 (= G230), A239 (= A255), G240 (= G256), F243 (≠ M259), N270 (≠ I281), G273 (= G284), A274 (= A285)
binding 5-aminoimidazole ribonucleoside: D241 (= D257)

Sites not aligning to the query:

active site: 24, 88
binding phosphomethylphosphonic acid adenylate ester: 149
binding 5-aminoimidazole ribonucleoside: 8, 12, 27, 88, 90, 151, 154

Q8ZKR2 Aminoimidazole riboside kinase; AIRs kinase; EC 2.7.1.223 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
31% identity, 35% coverage: 184:292/312 of query aligns to 185:292/319 of Q8ZKR2

query
sites
Q8ZKR2

L

I

M

C

K

K

P

V

S

S

L

A

D

D

E

E

L

L

G

C

T

Q

L

L

M

S

G

G

R

-

T

-

V

-

S

-

A

-

E

-

A

A

E

S

Q

H

E

W

Q

Q

A

D

L

A

R

R

S

Y

L

Y

I

L

R

R

Q

D

G

L

C
x
G

A

C

E

D

V

T

I

T

V

I

L

I

S

S

L

L

G

G

A

A

D

D

G

G

A

A

L

L

Y

L

A

I

Y

T

G

A

D

E

Q

G

V

E

G

F

R

H

L

F

R

P

T

A

P

P

E

R

V

V

P

D

V

V

A

V

S
x
D

A

T

V
x
T

G

G

A

A

G

G

D
|
D

S

A

M

F

L

V

G

G

A

G

I

L

V

L

L

F

A

T

M

L

A

S

-

R

-

A

-

N

-

C

-

W

D

D

G

H

R

A

S

L

I

L

P

A

E

E

A

A

V

I

C

S

Q

N

G

A

I

N

A

A

A

C

G

G

A

A

A

M

T
x
A

V

V

M

T

R
x
A

P

K

G
|
G

G213 (≠ C218) binding
D246 (≠ S251) binding
T248 (≠ V253) binding
D252 (= D257) binding
A287 (≠ T287) binding
A290 (≠ R290) binding
G292 (= G292) binding

Sites not aligning to the query:

16 binding
31 binding
101 binding
162 binding
180 binding
181 binding
183 binding

P76658 Bifunctional protein HldE; EC 2.7.1.167; EC 2.7.7.70 from Escherichia coli (strain K12) (see 2 papers)
26% identity, 57% coverage: 116:293/312 of query aligns to 135:300/477 of P76658

query
sites
P76658

Q

E

E

R

L

I

Q

N

Q

Q

C

A

L

L

D

S

S

S

L

I

A

G

A

A

L

L

R

-

P

-

A

-

P

-

A

-

Y

-

V

-

V

V

L

L

S

S

G

-

S

D

F

Y

P

A

P

K

G

G

A

A

D

-

L

L

G

A

F

S

F

V

D

Q

E

Q

L

M

L

I

A

Q

L

L

A

A

R

R

R

K

I

A

G

G

A

V

R

P

L

V

V

L

V

I

D

D

L

P

S
x
K

G

G

E

T

P

D

L

F

R

E

H

-

A

-

R

R

Q

Y

G

R

G

G

V

A

Y

T

L

L

M

L

K

T

P

P

S
x
N

L

L

D

S

E
|
E

L

F

G

E

T

A

L

V

M

V

G

G

R

K

T

C

V

K

S

T

A

E

E

E

A

E

E

I

Q

V

E

E

Q

R

A

G

L

M

R

K

S

L

L

I

I

A

R

D

Q

Y

G

E

C

L

A

S

E

A

V

L

I

L

V

V

L

T

S

R

L

S

G

E

A

Q

D

G

G

M

A

S

L

L

Y

L

A

Q

Y

P

G

G

D

K

Q

A

V

P

G

L

R

H

L

M

R

P

T

T

P

Q

E

A

V

Q

P

E

V

V

A

Y

S

D

A

V

V

T

G

G

A

A

G

G

D
|
D

S

T

M

V

L

I

G

G

A

V

I

L

V

A

L

A

A

T

M

L

A

A

D

A

G

G

R

N

S

S

I

L

P

E

E

E

A

A

V

C

C

F

Q

F

G

A

I

N

A

A

G

A

A

G

A

V

T

V

V

V

M

G

R

K

P

L

G

G

T

T

K179 (≠ S169) modified: N6-acetyllysine
N195 (≠ S188) mutation to D: Loss of activity.
E198 (= E191) mutation to D: Loss of activity.
D264 (= D257) mutation to E: Loss of activity.; mutation to N: Loss of activity.

Query Sequence

>GFF1892 FitnessBrowser__psRCH2:GFF1892
MPLIATLTLNPAMDLSVSTARVISTEKLRCSLPRHDPGGGGINVARVVKTLGGKAVAVYP
AGGPFGDLLQRSLDELGLVHRPVPIAGDTRESFTVDELASGLQYRFVLPGPTLSAQELQQ
CLDSLAALRPAPAYVVLSGSFPPGADLGFFDELLALARRIGARLVVDLSGEPLRHAARQG
GVYLMKPSLDELGTLMGRTVSAEAEQEQALRSLIRQGCAEVIVLSLGADGALYAYGDQVG
RLRTPEVPVASAVGAGDSMLGAIVLAMADGRSIPEAVCQGIAAGAATVMRPGTELCHRED
VQRLLQLMESAH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory