SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H32) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D36) mutation D->N,E: Little effect on the kinetic properties.
H81 (≠ F84) mutation to A: 5-fold decrease in glutamine affinity.
A105 (= A109) mutation to H: Little effect on the kinetic properties.
E349 (≠ D365) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 59% coverage: 1:374/632 of query aligns to 1:365/557 of P78753

query
sites
P78753

M

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C

C

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Y

Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
29% identity, 55% coverage: 30:374/632 of query aligns to 24:374/561 of P08243

query
sites
P08243

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V210 (= V213) to E: in dbSNP:rs1049674
F362 (≠ L362) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

2 active site, For GATase activity; C→A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
6 A → E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
29% identity, 55% coverage: 30:374/632 of query aligns to 23:361/509 of 6gq3A

query
sites
6gq3A

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active site: L49 (= L53), N74 (= N78), G75 (= G79), T324 (vs. gap), R327 (vs. gap)
binding 5-oxo-l-norleucine: R48 (= R52), V51 (≠ I55), V52 (≠ L56), Y73 (≠ F77), N74 (= N78), G75 (= G79), E76 (= E80), V95 (≠ T102), D96 (= D103)

Sites not aligning to the query:

active site: 4
binding 5-oxo-l-norleucine: 1

1mb9A Beta-lactam synthetase complexed with atp (see paper)
27% identity, 39% coverage: 76:323/632 of query aligns to 68:291/485 of 1mb9A

query
sites
1mb9A

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L

I

L

G

A

R

T

D

D

R

H

L

A

G

G

K

S

K

V

P

P

L

L

Y

Y

Y

T

T

C

D

V

T

A

D

P

G

G

Q

E

F

V

F

R

F

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

F

A

A

A

T

F

P

P

V

L

V

A

R

-

P

-

A

-

L

-

R

-

P

-

D

-

A

-

I

-

K

-

D

-

F

-

V

-

Y

-

Q

-

Y

-

I

-

P

-

D

D

P

A

K

R

T

R

I

V

-

A

-

G

Y

L

A

T

N

G

V

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

C

V

M

M

E

D

V

I

-

D

-

L

C

G

E

S

G

G

R

T

I

A

S

V

V

T

R

H

K

R

Y

T

W

W

D

T

-

P

-

G

L

L

S

S

F

R

R

R

P

I

V

L

-

P

E

E

G

G

R

E

T

A

V

V

S

A

D

A

I

V

K

R

A

A

G

A

L

L

Y

E

D

K

V

A

I

V

D

A

E

Q

A

R

V

V

R

-

L

-

R

-

M

T

I

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

G

A

L

-

M

C

A

A

G

H

R

R

S

A

Q

G

P

E

V

L

T

D

T

T

C

V

T
x
S

I
x
M

G

G

F

T

D

D

D

T

E

S

K

-

F

-

D

N

E

E

I

F

K

R

Y

E

A

A

D

R

L

A

V

V

A

V

R

D

Q

H

F

L

K

R

T

T

D

R

H

H

H

R

V

E

F

I

T

T

V

I

K

P

E

T

T

T

active site: A70 (≠ N78), G71 (= G79)
binding adenosine monophosphate: V235 (≠ F264), L236 (= L265), S242 (= S271), S260 (≠ T290), M261 (≠ I291)
binding adenosine-5'-triphosphate: V235 (≠ F264), L236 (= L265), S237 (= S266), G239 (= G268), D241 (= D270), S242 (= S271), S260 (≠ T290), M261 (≠ I291)
binding magnesium ion: D241 (= D270)
binding pyrophosphate 2-: S237 (= S266), G239 (= G268), D241 (= D270), S242 (= S271)

Sites not aligning to the query:

active site: 310, 336, 370, 431
binding adenosine monophosphate: 314, 318, 335, 336
binding adenosine-5'-triphosphate: 318, 335, 339, 411, 431
binding magnesium ion: 339
binding pyrophosphate 2-: 339, 411, 431

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
28% identity, 39% coverage: 76:323/632 of query aligns to 63:287/491 of 1mc1A

query
sites
1mc1A

V

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

R

D

S

E

L

L

R

L

E

S

G

V

L

L

E

P

Q

A

D

-

G

-

F

-

S

G

F

P

K

A

T

P

Q

E

T

G

D

D

T

A

E

E

V

L

L

V

L

L

A

R

L

L

Y

L

A

E

R

R

H

Y

G

D

E

L

S

H

C

A

L

F

R

R

Q

L

L

V

N

N

G

G

M

R

F

F

A

A

F

T

A

V

I

V

W

R

D

T

A

G

K

-

T

-

K

D

S

R

L

V

F

L

I

L

G

A

R

T

D

D

R

H

L

A

G

G

K

S

K

V

P

P

L

L

Y

Y

Y

T

T

C

D

V

T

A

D

P

G

G

Q

E

F

V

F

R

F

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

F

A

A

A

T

G

P

F

V

P

V

L

R

A

P

D

A

A

L

R

R

R

P

V

D

A

A

G

I

L

K

T

D

-

F

-

V

-

Y

-

Q

-

Y

-

I

-

P

-

D

-

P

-

K

-

T

-

I

-

Y

-

A

-

N

G

V

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

C

V

M

M

E

D

V

I

-

D

-

L

C

G

E

S

G

G

R

T

I

A

S

V

V

T

R

H

K

R

Y

T

W

W

D

T

-

P

-

G

L

L

S

S

F

R

R

R

P

I

V

L

-

P

E

E

G

G

R

E

T

A

V

V

S

A

D

A

I

V

K

R

A

A

G

A

L

L

Y

E

D

K

V

A

I

V

D

A

E

Q

A

R

V

V

R

-

L

-

R

-

M

T

I

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L

L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

G

A

L

-

M

C

A

A

G

H

R

R

S

A

Q

G

P

E

V

L

T

D

T

T

C

V

T
x
S

I
x
M

G

G

F

T

D

D

D

T

E

S

K

-

F

-

D

N

E

E

I

F

K

R

Y

E

A

A

D

R

L

A

V

V

A

V

R

D

Q

H

F

L

K

R

T

T

D

R

H

H

H

R

V

E

F

I

T

T

V

I

K

P

E

T

T

T

active site: A65 (≠ N78), G66 (= G79)
binding adenosine monophosphate: V231 (≠ F264), S233 (= S266), S238 (= S271), S256 (≠ T290), M257 (≠ I291)
binding magnesium ion: D237 (= D270)
binding pyrophosphate 2-: S233 (= S266), G235 (= G268), D237 (= D270), S238 (= S271)

Sites not aligning to the query:

active site: 306, 332, 366, 427
binding adenosine monophosphate: 331, 427, 430
binding magnesium ion: 335
binding deoxyguanidinoproclavaminic acid: 310, 332, 333, 336, 357, 366, 427
binding pyrophosphate 2-: 335, 407, 427, 428

1jgtB Crystal structure of beta-lactam synthetase (see paper)
27% identity, 39% coverage: 76:323/632 of query aligns to 71:300/500 of 1jgtB

query
sites
1jgtB

V

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

R

D

S

E

L

L

R

L

E

S

G

V

L

L

E

P

Q

A

D

-

G

-

F

-

S

G

F

P

K

A

T

P

Q

E

T

G

D

D

T

A

E

E

V

L

L

V

L

L

A

R

L

L

Y

L

A

E

R

R

H

Y

G

D

E

L

S

H

C

A

L

F

R

R

Q

L

L

V

N

N

G

G

M

R

F

F

A

A

F

T

A

V

I

V

W

R

D

T

A

G

K

-

T

-

K

D

S

R

L

V

F

L

I

L

G

A

R

T

D

D

R

H

L

A

G

G

K

S

K

V

P

P

L

L

Y

Y

Y

T

T

C

D

V

T

A

D

P

G

G

Q

E

F

V

F

R

F

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

F

A

A

A

T

H

P

-

V

-

R

-

P

-

A

-

L

-

R

-

P

-

D

-

A

-

I

-

K

R

D

D

F

P

F

K

V

G

Y

F

Q

P

Y

-

I

L

P

A

D

D

P

A

K

R

T

R

I

V

-

A

-

G

Y

L

A

T

N

G

V

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

C

V

M

M

E

D

V

I

-

D

-

L

C

G

E

S

G

G

R

T

I

A

S

V

V

T

R

H

K

R

Y

T

W

W

D

T

-

P

-

G

L

L

S

S

F

R

R

R

P

I

V

L

-

P

E

E

G

G

R

E

T

A

V

V

S

A

D

A

I

V

K

R

A

A

G

A

L

L

Y

E

D

K

V

A

I

V

D

A

E

Q

A

R

V

V

R

-

L

-

R

-

M

T

I

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

I
|
I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

G

A

L

-

M

C

A

A

G

H

R

R

S

A

Q

G

P

E

V

L

T

D

T

T

C

V

T
x
S

I
x
M

G

G

F

T

D

D

D

T

E

S

K

-

F

-

D

N

E

E

I

F

K

R

Y

E

A

A

D

R

L

A

V

V

A

V

R

D

Q

H

F

L

K

R

T

T

D

R

H

H

H

R

V

E

F

I

T

T

V

I

K

P

E

T

T

T

active site: A73 (≠ N78), G74 (= G79)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ F264), L245 (= L265), S246 (= S266), G248 (= G268), I249 (= I269), D250 (= D270), S251 (= S271), S269 (≠ T290), M270 (≠ I291)
binding magnesium ion: D250 (= D270)

Sites not aligning to the query:

active site: 319, 345, 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 327, 344, 345, 348, 420, 440
binding n2-(carboxyethyl)-l-arginine: 323, 345, 346, 348, 349, 354, 370, 379
binding magnesium ion: 348

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
27% identity, 39% coverage: 76:323/632 of query aligns to 67:292/496 of 1mbzA

query
sites
1mbzA

V

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

F

R

R

D

S

E

L

L

R

L

E

S

G

V

L

L

E

P

Q

A

D

-

G

-

F

-

S

G

F

P

K

A

T

P

Q

E

T

G

D

D

T

A

E

E

V

L

L

V

L

L

A

R

L

L

Y

L

A

E

R

R

H

Y

G

D

E

L

S

H

C

A

L

F

R

R

Q

L

L

V

N

N

G

G

M

R

F

F

A

A

F

T

A

V

I

V

W

R

D

T

A

G

K

-

T

-

K

D

S

R

L

V

F

L

I

L

G

A

R

T

D

D

R

H

L

A

G

G

K

S

K

V

P

P

L

L

Y

Y

Y

T

T

C

D

V

T

A

D

P

G

G

Q

E

F

V

F

R

F

A

G

S

S

T

E

E

I

A

K

K

A

A

L

L

F

-

A

-

T

-

P

-

V

-

R

-

P

-

A

-

L

-

R

-

P

-

D

-

A

A

I

A

K

H

D

G

F

F

F

-

V

-

Y

-

Q

-

Y

P

I

L

P

A

D

D

P

A

K

R

T

R

I

V

-

A

-

G

Y

L

A

T

N

G

V

V

H

Y

K

Q

L

V

P

P

P

A

G

G

H

A

C

V

M

M

E

D

V

I

-

D

-

L

C

G

E

S

G

G

R

T

I

A

S

V

V

T

R

H

K

R

Y

T

W

W

D

T

-

P

-

G

L

L

S

S

F

R

R

R

P

I

V

L

-

P

E

E

G

G

R

E

T

A

V

V

S

A

D

A

I

V

K

R

A

A

G

A

L

L

Y

E

D

K

V

A

I

V

D

A

E

Q

A

R

V

V

R

-

L

-

R

-

M

T

I

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

I

I

D
|
D

S
|
S

S

S

A

G

V

V

V

A

G

A

L

-

M

C

A

A

G

H

R

R

S

A

Q

G

P

E

V

L

T

D

T

T

C

V

T
x
S

I
x
M

G

G

F

T

D

D

D

T

E

S

K

-

F

-

D

N

E

E

I

F

K

R

Y

E

A

A

D

R

L

A

V

V

A

V

R

D

Q

H

F

L

K

R

T

T

D

R

H

H

H

R

V

E

F

I

T

T

V

I

K

P

E

T

T

T

active site: A69 (≠ N78), G70 (= G79)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ F264), L237 (= L265), S238 (= S266), S243 (= S271), S261 (≠ T290), M262 (≠ I291)
binding magnesium ion: D242 (= D270)
binding pyrophosphate 2-: S238 (= S266), G240 (= G268), D242 (= D270), S243 (= S271)

Sites not aligning to the query:

active site: 311, 337, 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 315, 319, 336, 337, 338, 340, 341, 362, 371, 432, 434, 435
binding magnesium ion: 340
binding pyrophosphate 2-: 340, 412, 432, 433

Q9STG9 Amidophosphoribosyltransferase 2, chloroplastic; AtATase2; AtPURF2; PRPP2; Glutamine phosphoribosylpyrophosphate amidotransferase 2; AtGPRAT2; Protein CHLOROPLAST IMPORT APPARATUS 1; Protein DIFFERENTIAL DEVELOPMENT OF VASCULAR ASSOCIATED CELLS; EC 2.4.2.14 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
32% identity, 20% coverage: 45:168/632 of query aligns to 152:285/561 of Q9STG9

query
sites
Q9STG9

D

D

V

I

A

A

L

I

V

G

H

H

Q

V

R

R

L

Y

S

S

I

T

L

A

D

G

L

S

S

S

S

M

A

L

G

K

N

N

-

V

Q

Q

P

P

M

F

A

V

S

A

S

G

C

Y

-

R

-

F

G

G

R

S

Y

V

I

G

I

V

V

A

F
x
H

N

N

G

G

E

N

I

L

Y

V

N

N

F

Y

R

T

S

K

L

L

R

R

E

A

G

D

L

L

E

E

Q

E

D

N

G

G

F

S

S

I

F

F

K

N

T

T

Q

S

T

S

D

D

T

T

E

E

V

V

L

V

L

L

A

H

L

L

Y

I

A

A

-

I

-

S

-

K

-

A

-

R

-

P

-

F

-

M

R

R

H

I

G

V

E

D

S

A

C

C

L

-

R

E

Q

K

L

L

N

Q

G

G

M

-

F

-

A

A

F

Y

A

S

I

M

W

V

D

F

A

V

K

T

T

E

K

D

S

K

L

L

F

V

I

A

G

V

R

R

D

D

R

P

L

H

G

G

K

F

K
x
R

P
|
P

L

L

Y

V

Y

M

-

G

T

R

D

R

T

S

D

N

G

G

Q

A

F

V

F

F

G

A

S

S

E

E

I

T

K

C

A

A

L

L

H187 (≠ F77) mutation to T: In cia1-2; small plants with white leaves showing an irregular mosaic of green sectors.
R264 (≠ K148) mutation to K: Strong resistance to the bleaching herbicides DAS073 and DAS734.
P265 (= P149) mutation to S: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with F-494.

Sites not aligning to the query:

371 G→S: Low resistance to the bleaching herbicides DAS073 and DAS734.
476 P→S: Resistance to the bleaching herbicides DAS073 and DAS734.
494 Y→F: Low resistance to the bleaching herbicides DAS073 and DAS734; when associated with S-265.

6lbpA Structure of the glutamine phosphoribosylpyrophosphate amidotransferase from arabidopsis thaliana (see paper)
32% identity, 20% coverage: 45:168/632 of query aligns to 66:199/460 of 6lbpA

query
sites
6lbpA

D

D

V

I

A

A

L

I

V

G

H

H

Q

V

R

R

L

Y

S

S

I

T

L

A

D

G

L

S

S

S

S

M

A

L

G

K

N

N

-

V

Q

Q

P

P

M

F

A

V

S

A

S

G

C

Y

-

R

-

F

G

G

R

S

Y

V

I

G

I

V

V

A

F

H

N
|
N

G
|
G

E

N

I

L

Y

V

N

N

F

Y

R

T

S

K

L

L

R

R

E

A

G

D

L

L

E

E

Q

E

D

N

G

G

F

S

S

I

F

F

K

N

T

T

Q

S

T

S

D

D

T

T

E

E

V

V

L

V

L

L

A

H

L

L

Y

I

A

A

-

I

-

S

-

K

-

A

-

R

-

P

-

F

-

M

R

R

H

I

G

V

E

D

S

A

C

C

L

-

R

E

Q

K

L

L

N

Q

G

G

M

-

F

-

A

A

F

Y

A

S

I

M

W

V

D

F

A

V

K

T

T

E

K

D

S

K

L

L

F

V

I

A

G

V

R

R

D

D

R

P

L

H

G

G

K

F

K

R

P

P

L

L

Y

V

Y

M

-

G

T

R

D

R

T

S

D

N

G

G

Q

A

F

V

F

F

G

A

S

S

E

E

I

T

K

C

A

A

L

L

active site: N102 (= N78), G103 (= G79)

Sites not aligning to the query:

active site: 1, 27, 243, 301, 306, 316, 424
binding iron/sulfur cluster: 237, 239, 383, 385, 434, 436, 437

Query Sequence

>GFF1957 FitnessBrowser__Marino:GFF1957
MCGIAGFLRTGTLPDREQHHLWAERMGQAIAHRGPDANGVHIDQDVALVHQRLSILDLSS
AGNQPMASSCGRYIIVFNGEIYNFRSLREGLEQDGFSFKTQTDTEVLLALYARHGESCLR
QLNGMFAFAIWDAKTKSLFIGRDRLGKKPLYYTDTDGQFFFGSEIKALFATPVVRPALRP
DAIKDFFVYQYIPDPKTIYANVHKLPPGHCMEVCEGRISVRKYWDLSFRPVEGRTVSDIK
AGLYDVIDEAVRLRMISDVPLGAFLSGGIDSSAVVGLMAGRSSQPVTTCTIGFDDEKFDE
IKYADLVARQFKTDHHVFTVKETVADNLVGISRFFDEPFADPSFVPTFFVSQLARTQVTV
ALAGDGGDENFAGYSKYRTDAIENRIRSLFPPALRHSLFPGLSRLAGHIPGPLGKKASSL
LGTVALDPDLGFFTSNCFFNPRVWKRVVSPEFAALTDDYDPADITRHHYQEAPAEDHLSR
ILYTDIKTYLPGDILVKVDRMSMANSLETRAPLLDYRVVEYAAGIPSALKLKGNCKKHVL
KECFSDLLDEDILYRKKMGFSVPLAQWLRSEIRAIAEPLLTGPDSGLSRYFRMEQVRALW
QAHLSGDNRFTQELWSMVVFALWYQHYKDFVR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory