SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2001 FitnessBrowser__Phaeo:GFF2001 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P18079 5-aminolevulinate synthase; 5-aminolevulinic acid synthase; Delta-ALA synthase; Delta-aminolevulinate synthase; EC 2.3.1.37 from Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) (see paper)
79% identity, 99% coverage: 3:406/409 of query aligns to 1:406/409 of P18079

query
sites
P18079

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R21 (= R23) binding
S137 (= S139) binding
K156 (= K158) binding
S189 (= S191) binding in other chain
H217 (= H219) binding in other chain
T245 (= T247) binding in other chain
K248 (= K250) active site
S277 (≠ T279) binding
T278 (= T280) binding
T365 (= T365) binding

2bwoB 5-aminolevulinate synthase from rhodobacter capsulatus in complex with succinyl-coa (see paper)
79% identity, 97% coverage: 3:398/409 of query aligns to 1:398/399 of 2bwoB

query
sites
2bwoB

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active site: N54 (= N56), H142 (= H144), E185 (= E187), S189 (= S191), D214 (= D216), H217 (= H219), K248 (= K250)
binding pyridoxal-5'-phosphate: S114 (= S116), A115 (= A117), Y116 (= Y118), H142 (= H144), E185 (= E187), S189 (= S191), D214 (= D216), V216 (= V218), H217 (= H219), T245 (= T247), K248 (= K250)
binding succinyl-coenzyme a: R21 (= R23), T83 (= T85), N85 (= N87), S137 (= S139), S139 (≠ A141), H142 (= H144), I146 (= I148), K150 (≠ R152), K156 (= K158), I158 (= I160), F276 (= F278), F363 (= F363), P364 (= P364), T365 (= T365)

2bwpA 5-aminolevulinate synthase from rhodobacter capsulatus in complex with glycine (see paper)
79% identity, 97% coverage: 3:398/409 of query aligns to 1:398/398 of 2bwpA

query
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active site: N54 (= N56), H142 (= H144), E185 (= E187), S189 (= S191), D214 (= D216), H217 (= H219), K248 (= K250)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: N54 (= N56), A115 (= A117), Y116 (= Y118), H142 (= H144), E185 (= E187), D214 (= D216), V216 (= V218), H217 (= H219), T245 (= T247), K248 (= K250), S277 (≠ T279), T278 (= T280)

P22557 5-aminolevulinate synthase, erythroid-specific, mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2; EC 2.3.1.37 from Homo sapiens (Human) (see 11 papers)
50% identity, 98% coverage: 1:402/409 of query aligns to 137:545/587 of P22557

query
sites
P22557

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Y

Y

R

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N

A

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P

P

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A

A

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-

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A

K

S

Q

K

D

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I

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W

W

C

C

G

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N

N

D

D

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Y

L

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R

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x
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N

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G

G

A

A

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x
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N

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G

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Y

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Q

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A

I

I

N

N

F

Y

P

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|
R

G

G

T

E

E

E

R

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R

F

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A

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|
P

S

S

P

P

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S

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G

L

L

K156 (≠ L16) to E: in SIDBA1; significantly reduced activity
R170 (= R30) to H: in SIDBA1; significantly increased thermosensitivity
R204 (≠ Q63) to Q: in SIDBA1; 15% to 35% activity of wild-type; dbSNP:rs1338391423
R218 (≠ A77) to H: in SIDBA1; significantly increased thermosensitivity; dbSNP:rs185504937
E242 (= E101) to K: in SIDBA1; significantly reduced activity
C258 (≠ A117) binding in other chain
F259 (≠ Y118) binding in other chain
D263 (= D122) to N: in SIDBA1; significantly reduced activity
P339 (= P198) to L: in SIDBA1; significantly reduced activity
H360 (= H219) binding in other chain
R375 (= R234) to C: in SIDBA1; significantly reduced activity
T388 (= T247) binding in other chain
K391 (= K250) modified: N6-(pyridoxal phosphate)lysine
R411 (= R270) to C: in SIDBA1; 12% to 25% activity of wild-type; dbSNP:rs137852305; to H: in SIDBA1; significantly reduced activity
T420 (= T279) binding
T421 (= T280) binding
R452 (≠ L309) to G: in SIDBA1; does not affect activity
R511 (= R368) mutation to E: 2-fold increased enzyme activity with a lower specificity for glycine and higher for succinyl-CoA.
P520 (= P377) to L: in SIDBA1; likely benign; associated in cis with H-560 in a patient; dbSNP:rs201062903

Sites not aligning to the query:

1:49 modified: transit peptide, Mitochondrion
560 R → H: in SIDBA1; associated in cis with L-520 in a patient; dbSNP:rs892041887
572 R → H: in SIDBA1; does not affect activity
586 Y → F: rare variant found in a congenital erythropoietic porphyria patient that also carries UROS mutations R-73 and Q-248; increased enzyme activity; dbSNP:rs139596860

P08680 5-aminolevulinate synthase, erythroid-specific, mitochondrial; ALAS-E; 5-aminolevulinic acid synthase 2; Delta-ALA synthase 2; Delta-aminolevulinate synthase 2; EC 2.3.1.37 from Mus musculus (Mouse) (see 2 papers)
52% identity, 93% coverage: 22:402/409 of query aligns to 162:545/587 of P08680

query
sites
P08680

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

N

Q

A

F

Y

P

P

H

F

A

A

V

Q

-

H

W

F

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E

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A

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S

G

M

S

A

K

S

Q

K

D

D

I

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T

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V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

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G

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I

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S

Q

R

H

H

P

P

V

R

V

V

L

L

E

Q

A

A

M

I

H

E

G

E

A

T

I

L

D

K

A

N

T

H

G

G

A

A

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S

S

A

C

Y

F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

L

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H

H

A

A

S

S

M

M

I

I

E

Q

G

G

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I

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R

R

N

N

S

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G

G

A

A

A

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K

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A

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A

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A

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G

A

A

A

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A

E

S

S

V

V

A

R

Y

L

L

L

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K

-

G

-

E

T

E

A

G

P

Q

E

A

L

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E

R

Q

A

H

H

Q

Q

L

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N

A

V

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M

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R

M

Q

R

L

L

L

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I

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S

H

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P

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I

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R

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G

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N

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H

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Y

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Q

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A

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N

F

Y

P

P

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T

V

V

P

P

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G

G

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E

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R

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L

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R

F

L

T

A

P

P

S

S

P

P

V

H

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S

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Q

E

M

I

M

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E

A

N

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A

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D

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A

L

L

A

W

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A

G

L

L

K391 (= K250) modified: N6-(pyridoxal phosphate)lysine; mutation K->A,H: Abolishes enzyme activity.

Sites not aligning to the query:

1:49 modified: transit peptide, Mitochondrion

5qrdB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1328968520
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/428 of 5qrdB

query
sites
5qrdB

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N
|
N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

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A

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D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S

S

M

M

D

D

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G

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A

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I

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C

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P

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L

A

E

E

E

I

L

C

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L

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A

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E

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E

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F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding pyridoxal-5'-phosphate: S116 (= S116), C117 (≠ A117), F118 (≠ Y118), N121 (= N121), H144 (= H144), E187 (= E187), D216 (= D216), V218 (= V218), H219 (= H219), T247 (= T247), K250 (= K250), T279 (= T279), T280 (= T280)

Sites not aligning to the query:

binding 4-[(2-methylsulfonylimidazol-1-yl)methyl]-1,3-thiazole: 423, 424

5qrbB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z2856434868
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/428 of 5qrbB

query
sites
5qrbB

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q
x
W

N

A

G

D

Q
x
A

F

Y

P

P

H
x
F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D
|
D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding 1-ethyl-N-(2-fluorophenyl)piperidin-4-amine: W35 (≠ Q31), A38 (≠ Q34), F41 (≠ H37), D49 (= D49)
binding pyridoxal-5'-phosphate: S116 (= S116), C117 (≠ A117), F118 (≠ Y118), H144 (= H144), E187 (= E187), S191 (= S191), D216 (= D216), V218 (= V218), H219 (= H219), T247 (= T247), K250 (= K250), T279 (= T279), T280 (= T280)

5qr8B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z57258487
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/428 of 5qr8B

query
sites
5qr8B

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S

S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding pyridoxal-5'-phosphate: C117 (≠ A117), F118 (≠ Y118), H144 (= H144), E187 (= E187), S191 (= S191), D216 (= D216), V218 (= V218), H219 (= H219), T247 (= T247), K250 (= K250), T279 (= T279), T280 (= T280)

Sites not aligning to the query:

binding N-{[4-(dimethylamino)phenyl]methyl}-4H-1,2,4-triazol-4-amine: 6, 7, 8, 10

5qr7B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z57299529
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/428 of 5qr7B

query
sites
5qr7B

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q
x
W

N

A

G

D

Q
x
A

F

Y

P

P

H
x
F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D
|
D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S

S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S

S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E
x
H

E

Q

F

Y

G

G

A

A

L
|
L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D
x
H

R

K

I

I

D
|
D

I

I

V

I

N

S

G

G

T

T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S
x
T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding ~{N}-(2-phenylethyl)-1~{H}-benzimidazol-2-amine: W35 (≠ Q31), A38 (≠ Q34), F41 (≠ H37), D49 (= D49), H207 (≠ E207), L212 (= L212), H239 (≠ D239), D242 (= D242), T262 (≠ S262)
binding pyridoxal-5'-phosphate: C117 (≠ A117), F118 (≠ Y118), H144 (= H144), E187 (= E187), D216 (= D216), V218 (= V218), H219 (= H219), K250 (= K250), T279 (= T279), T280 (= T280)

5qr5B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z2241115980
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/428 of 5qr5B

query
sites
5qr5B

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q
x
W

N

A

G

D

Q

A

F

Y

P

P

H
x
F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D
|
D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N
|
N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T

T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding 1-[(furan-2-yl)methyl]-4-(methylsulfonyl)piperazine: W35 (≠ Q31), F41 (≠ H37), D49 (= D49)
binding pyridoxal-5'-phosphate: S116 (= S116), C117 (≠ A117), F118 (≠ Y118), N121 (= N121), H144 (= H144), E187 (= E187), S191 (= S191), D216 (= D216), V218 (= V218), H219 (= H219), K250 (= K250), T279 (= T279), T280 (= T280)

5qr4B Pandda analysis group deposition -- crystal structure of human alas2a in complex with z2856434826
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/428 of 5qr4B

query
sites
5qr4B

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q
x
W

N

A

G
x
D

Q
x
A

F

Y

P

P

H
x
F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S

S

A
x
C

Y
x
F

I

V

A

A

N
|
N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding [4-(propan-2-yl)piperazin-1-yl](thiophen-2-yl)methanone: W35 (≠ Q31), D37 (≠ G33), A38 (≠ Q34), F41 (≠ H37)
binding pyridoxal-5'-phosphate: C117 (≠ A117), F118 (≠ Y118), N121 (= N121), H144 (= H144), E187 (= E187), S191 (= S191), D216 (= D216), V218 (= V218), H219 (= H219), T247 (= T247), K250 (= K250), T279 (= T279), T280 (= T280)

5qqzB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1675346324
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/428 of 5qqzB

query
sites
5qqzB

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q
x
W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D
|
D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S

S

A
x
C

Y
x
F

I

V

A

A

N
|
N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S

S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T

T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding trans-3-[(2,6-dimethylpyrimidin-4-yl)(methyl)amino]cyclobutan-1-ol: W35 (≠ Q31), D49 (= D49)
binding pyridoxal-5'-phosphate: C117 (≠ A117), F118 (≠ Y118), N121 (= N121), H144 (= H144), E187 (= E187), D216 (= D216), V218 (= V218), H219 (= H219), K250 (= K250), T279 (= T279), T280 (= T280)

5qqrB Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1171217421
52% identity, 93% coverage: 22:402/409 of query aligns to 26:404/429 of 5qqrB

query
sites
5qqrB

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

Q

D

H

G

F

S

S

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding pyridoxal-5'-phosphate: S116 (= S116), C117 (≠ A117), F118 (≠ Y118), H144 (= H144), E187 (= E187), S191 (= S191), D216 (= D216), V218 (= V218), H219 (= H219), T247 (= T247), K250 (= K250), T279 (= T279), T280 (= T280)

Sites not aligning to the query:

binding 1-[(2-methyl-1,3-thiazol-5-yl)methyl]piperazine: 407, 408, 409

6hrhA Structure of human erythroid-specific 5'-aminolevulinate synthase, alas2
52% identity, 93% coverage: 22:402/409 of query aligns to 26:403/429 of 6hrhA

query
sites
6hrhA

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

-

D

Q

G

H

S

F

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V

V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding pyridoxal-5'-phosphate: S115 (= S116), C116 (≠ A117), F117 (≠ Y118), H143 (= H144), E186 (= E187), S190 (= S191), D215 (= D216), H218 (= H219), T246 (= T247), K249 (= K250), T278 (= T279), T279 (= T280)

5qreA Pandda analysis group deposition -- crystal structure of human alas2a in complex with z117233350
52% identity, 93% coverage: 22:402/409 of query aligns to 26:403/429 of 5qreA

query
sites
5qreA

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

-

D

Q

G

H

S

F

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S

S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding pyridoxal-5'-phosphate: C116 (≠ A117), F117 (≠ Y118), H143 (= H144), E186 (= E187), S190 (= S191), D215 (= D216), V217 (= V218), H218 (= H219), T246 (= T247), K249 (= K250), T278 (= T279), T279 (= T280)

Sites not aligning to the query:

binding 3-ethyl-5-methyl-N-(5-methyl-1,2-oxazol-3-yl)-1,2-oxazole-4-carboxamide: 424, 425

5qrcA Pandda analysis group deposition -- crystal structure of human alas2a in complex with z31721798
52% identity, 93% coverage: 22:402/409 of query aligns to 26:403/429 of 5qrcA

query
sites
5qrcA

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

-

D

Q

G

H

S

F

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K
|
K

L
x
I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T

T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y
|
Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding 3-cyclohexyl-1-(morpholin-4-yl)propan-1-one: K129 (= K130), I130 (≠ L131), Y271 (= Y272)
binding pyridoxal-5'-phosphate: S115 (= S116), C116 (≠ A117), F117 (≠ Y118), H143 (= H144), E186 (= E187), S190 (= S191), D215 (= D216), V217 (= V218), H218 (= H219), K249 (= K250), T278 (= T279), T279 (= T280)

5qraA Pandda analysis group deposition -- crystal structure of human alas2a in complex with z1101755952
52% identity, 93% coverage: 22:402/409 of query aligns to 26:403/429 of 5qraA

query
sites
5qraA

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

-

D

Q

G

H

S

F

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K
|
K

L
x
I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T
|
T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y
|
Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding [(4R)-4-methyl-2,3,4,5-tetrahydro-1H-azepin-1-yl](1,3-thiazol-4-yl)methanone: K129 (= K130), I130 (≠ L131), Y271 (= Y272)
binding pyridoxal-5'-phosphate: S115 (= S116), C116 (≠ A117), F117 (≠ Y118), H143 (= H144), E186 (= E187), S190 (= S191), D215 (= D216), V217 (= V218), H218 (= H219), T246 (= T247), K249 (= K250), T278 (= T279), T279 (= T280)

5qr9A Pandda analysis group deposition -- crystal structure of human alas2a in complex with z31478129
52% identity, 93% coverage: 22:402/409 of query aligns to 26:403/429 of 5qr9A

query
sites
5qr9A

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

-

D

Q

G

H

S

F

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E
x
H

E

Q

F

Y

G

G

A

A

L
|
L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D
|
D

I

I

V

I

N

S

G

G

T

T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding ethyl benzylcarbamate: H206 (≠ E207), L211 (= L212), D241 (= D242)
binding pyridoxal-5'-phosphate: S115 (= S116), C116 (≠ A117), F117 (≠ Y118), H143 (= H144), E186 (= E187), S190 (= S191), D215 (= D216), V217 (= V218), H218 (= H219), K249 (= K250), T278 (= T279), T279 (= T280)

5qr3A Pandda analysis group deposition -- crystal structure of human alas2a in complex with z915492990
52% identity, 93% coverage: 22:402/409 of query aligns to 26:403/429 of 5qr3A

query
sites
5qr3A

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

-

D

Q

G

H

S

F

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

A

G

G

T

T

R

R

N

N

I

I

S

S

G

G

T

T

T

S

V

K

Y

F

H

H

K

V

Q

E

L

L

E

E

A

Q

E

E

L

L

A

A

D

E

L

L

H

H

Q

Q

K

K

E

D

A

S

A

A

L

L

F

F

T

S

S
|
S

A
x
C

Y
x
F

I

V

A

A

N

N

D

D

A

S

T

T

L

L

S

F

T

T

L

L

P

A

K

K

L

I

F

L

P

P

G

G

L

C

I

E

I

I

F

Y

S

S

D

D

A

A

L

G

N

N

H
|
H

A

A

S

S

M

M

I

I

E

Q

G

G

V

I

R

R

R

N

N

S

G

G

G

A

A

A

K

K

R

F

I

V

F

F

R

R

H

H

N

N

D

D

V

P

A

D

H

H

L

L

R

K

E

K

L

L

A

E

A

K

A

S

D

N

P

P

A

K

A

I

P

P

K

K

L

I

I

V

A

A

F

F

E
|
E

S

T

V

V

Y

H

S
|
S

M

M

D

D

G

G

D

A

F

I

G

C

P

P

I

L

A

E

E

E

I

L

C

C

D

D

L

V

A

S

E

H

E

Q

F

Y

G

G

A

A

L

L

T

T

Y

F

I

V

D
|
D

E

E

V
|
V

H
|
H

A

A

V

V

G

G

M

L

Y

Y

G

G

A

S

R

R

G

G

G

A

G

G

V

I

T

G

E

E

R

R

D

D

G

G

L

I

I

M

D

H

R

K

I

I

D

D

I

I

V

I

N

S

G

G

T

T

L

L

A

G

K
|
K

A

A

Y

F

G

G

V

C

M

V

G

G

Y

Y

I

I

A

A

A

S

S

T

A

R

K

D

M

L

C

V

D

D

A

M

I

V

R

R

S

S

Y

Y

A

A

P

A

G

G

F

F

I

I

F

F

T
|
T

S

S

L

L

A

P

P

P

A

M

V

V

A

L

A

S

G

G

A

A

A

L

A

E

S

S

V

V

A

R

Y

L

L

L

K

K

-

G

-

E

T

E

A

G

P

Q

E

A

L

L

R

R

E

R

Q

A

H

H

Q

Q

L

R

Q

N

A

V

R

K

I

H

L

M

K

R

M

Q

R

L

L

L

K

M

G

D

L

R

G

G

L

L

P

P

I

V

I

I

D

P

H

C

G

P

S

S

H

H

I

I

V

I

P

P

V

I

I

R

V

V

G

G

N

N

P

A

V

A

H

L

T

N

Q

S

K

K

L

L

S

C

D

D

M

L

L

L

S

S

D

K

H

H

G

G

I

I

Y

Y

V

V

Q

Q

P

A

I

I

N

N

F

Y

P

P

T

T

V

V

P

P

R

R

G

G

T

E

E

E

R

L

L

L

R

R

F

L

T

A

P

P

S

S

P

P

V

H

H

H

G

S

P

P

K

Q

E

M

I

M

D

E

A

D

L

F

V

V

K

E

A

K

M

L

D

L

A

L

L

A

W

W

S

T

H

A

C

V

A

G

L

L

binding pyridoxal-5'-phosphate: S115 (= S116), C116 (≠ A117), F117 (≠ Y118), H143 (= H144), E186 (= E187), S190 (= S191), D215 (= D216), V217 (= V218), H218 (= H219), K249 (= K250), T278 (= T279), T279 (= T280)

Sites not aligning to the query:

binding 1-methyl-N-[(thiophen-2-yl)methyl]-1H-pyrazole-5-carboxamide: 6, 7

5qr1A Pandda analysis group deposition -- crystal structure of human alas2a in complex with z396380540
52% identity, 93% coverage: 22:402/409 of query aligns to 26:403/429 of 5qr1A

query
sites
5qr1A

Y

Y

R

R

T

V

F

F

I

K

D

T

I

V

E

N

R

R

Q

W

N

A

G

D

Q

A

F

Y

P

P

H

F

A

A

V

-

W

-

T

-

R

-

P

-

D

Q

G

H

S

F

K

S

Q

K

D

D

I

V

T

S

V

V

W

W

C

C

G

S

N

N

D

D

Y

Y

L

L

G

G

M

M

G

S

Q

R

H

H

P

P

V

Q

V

V

L

L

E

Q

A

A

M

T

H

Q

G

E

A

T

I

L

D

Q

A

R

T

H

G

G

A

V

G

G

S

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binding ~{N}-(cyclobutylmethyl)-1,5-dimethyl-pyrazole-4-carboxamide: I130 (≠ L131), Y271 (= Y272)
binding pyridoxal-5'-phosphate: S115 (= S116), C116 (≠ A117), F117 (≠ Y118), H143 (= H144), E186 (= E187), S190 (= S191), D215 (= D216), V217 (= V218), H218 (= H219), T246 (= T247), K249 (= K250), T278 (= T279), T279 (= T280)

Query Sequence

>GFF2001 FitnessBrowser__Phaeo:GFF2001
MPVDYTAKLDQAIDQLHTEGRYRTFIDIERQNGQFPHAVWTRPDGSKQDITVWCGNDYLG
MGQHPVVLEAMHGAIDATGAGSGGTRNISGTTVYHKQLEAELADLHQKEAALLFTSAYIA
NDATLSTLPKLFPGLIIFSDALNHASMIEGVRRNGGAKRIFRHNDVAHLRELLAAADPAA
PKLIAFESVYSMDGDFGPIAEICDLAEEFGALTYIDEVHAVGMYGARGGGVTERDGLIDR
IDIVNGTLAKAYGVMGGYIAASAKMCDAIRSYAPGFIFTTSLAPAVAAGAAASVAYLKTA
PELREQHQLQARILKMRLKGLGLPIIDHGSHIVPVIVGNPVHTQKLSDMLLSDHGIYVQP
INFPTVPRGTERLRFTPSPVHGPKEIDALVKAMDALWSHCALNRAEMAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory