SitesBLAST

P9WIC7 Glucosyl-3-phosphoglycerate phosphatase; Mannosyl-3-phosphoglycerate phosphatase; EC 3.1.3.85; EC 3.1.3.70 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
33% identity, 74% coverage: 12:153/193 of query aligns to 8:160/223 of P9WIC7

query
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P9WIC7

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R10 (= R14) mutation to A: Loss of phosphatase activity.
H11 (= H15) active site, Tele-phosphohistidine intermediate; mutation to A: Almost completely abolished phosphatase activity.
N17 (= N21) mutation to A: About 5% of wild-type phosphatase activity.
K47 (≠ Q55) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)
R60 (= R66) mutation to A: Loss of phosphatase activity.
H159 (= H152) mutation to A: About 5% of wild-type phosphatase activity.

Sites not aligning to the query:

209 L→E: Disrupts dimerization of the enzyme, which exists as a monomer and has lost its ability to perform dephosphorylation.

4qihA The structure of mycobacterial glucosyl-3-phosphoglycerate phosphatase rv2419c complexes with vo3 (see paper)
33% identity, 74% coverage: 12:153/193 of query aligns to 6:158/209 of 4qihA

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active site: H9 (= H15), R58 (= R66), E82 (= E88)
binding vanadate ion: R8 (= R14), H9 (= H15), Q21 (= Q27), R58 (= R66), H157 (= H152)

5zr2C Crystal structure of phosphoserine phosphatase mutant (h9a) from entamoeba histolytica in complex with phosphoserine (see paper)
28% identity, 84% coverage: 9:170/193 of query aligns to 3:161/198 of 5zr2C

query
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5zr2C

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binding phosphoserine: R8 (= R14), Q21 (= Q27), G22 (= G28), R57 (= R66), E79 (= E88), H146 (= H152), G147 (= G153)

4pzaB The complex structure of mycobacterial glucosyl-3-phosphoglycerate phosphatase rv2419c with inorganic phosphate (see paper)
33% identity, 74% coverage: 12:153/193 of query aligns to 7:159/217 of 4pzaB

query
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4pzaB

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active site: H10 (= H15), R59 (= R66), E83 (= E88)
binding phosphate ion: R9 (= R14), H10 (= H15), R59 (= R66), E83 (= E88), H158 (= H152), G159 (= G153)

5pgmE Saccharomyces cerevisiae phosphoglycerate mutase (see paper)
34% identity, 52% coverage: 8:108/193 of query aligns to 1:106/234 of 5pgmE

query
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5pgmE

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active site: H8 (= H15), R59 (= R66), E86 (= E88)

Sites not aligning to the query:

active site: 181
binding alanine: 231, 234

1bq4A Saccharomyces cerevisiae phosphoglycerate mutase in complex with benzene hexacarboxylate (see paper)
34% identity, 52% coverage: 8:108/193 of query aligns to 1:106/234 of 1bq4A

query
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1bq4A

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active site: H8 (= H15), R59 (= R66), E86 (= E88)
binding benzene hexacarboxylic acid: Y89 (≠ A91)

Sites not aligning to the query:

active site: 181
binding benzene hexacarboxylic acid: 113, 114, 183, 204

1bq3A Saccharomyces cerevisiae phosphoglycerate mutase in complex with inositol hexakisphosphate (see paper)
34% identity, 52% coverage: 8:108/193 of query aligns to 1:106/234 of 1bq3A

query
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1bq3A

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active site: H8 (= H15), R59 (= R66), E86 (= E88)
binding inositol hexakisphosphate: R7 (= R14), S11 (≠ T18), N14 (= N21), L18 (≠ R25), F19 (≠ I26), T20 (≠ Q27), Y89 (≠ A91), K97 (≠ R99)

Sites not aligning to the query:

active site: 181
binding inositol hexakisphosphate: 113

1qhfA Yeast phosphoglycerate mutase-3pg complex structure to 1.7 a (see paper)
34% identity, 52% coverage: 8:108/193 of query aligns to 1:106/240 of 1qhfA

query
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active site: H8 (= H15), R59 (= R66), E86 (= E88)
binding 3-phosphoglyceric acid: R7 (= R14), H8 (= H15), G9 (= G16), Q10 (= Q17), S11 (≠ T18), N14 (= N21), T20 (≠ Q27), R59 (= R66)

Sites not aligning to the query:

active site: 181
binding 3-phosphoglyceric acid: 207

P00950 Phosphoglycerate mutase 1; PGAM 1; BPG-dependent PGAM 1; MPGM 1; Phosphoglyceromutase 1; EC 5.4.2.11 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 7 papers)
34% identity, 52% coverage: 8:108/193 of query aligns to 2:107/247 of P00950

query
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P00950

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I

F

Q
x
T

G
|
G

Q

W

L

V

E

D

S

V

R

K

L

L

S

S

P

A

L

K

G

G

I

Q

E

Q

H

E

A

A

Q

A

Q

R

Q

A

A

G

G

E

L

L

M

L

A

K

P

E

I

K

L

K

A

V

Q

Y

G

P

P

D

A

V

C

L

Y

Y

V

T

S

S

P

K

L

L

G

S

R
|
R

A

A

Q

I

Q

Q

T

T

A

A

R

N

I

I

A

A

L

L

-

E

-

K

G

A

D

D

Q

R

-

L

-

W

-

I

P

P

F

V

I

N

T

R

D

S

A

W

R

R

L

L

A

N

E
|
E

A
x
R

Q
x
H

A
x
Y

G

G

V

D

F

L

Q

Q

G

G

L

K

T

D

R
x
K

Q

A

E

E

V

T

A

L

A

K

E

K

Y

F

P

G

E

E

8:15 (vs. 14:21, 88% identical) binding
H9 (= H15) active site, Tele-phosphohistidine intermediate
TG 21:22 (≠ QG 27:28) binding
R60 (= R66) binding
ERHY 87:90 (≠ EAQA 88:91) binding
K98 (≠ R99) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
114:115 binding
169 K→P: Causes dissociation of the homotetramer to dimers at low concentrations.
182 H→A: Reduces kcat of the mutase reaction 10000-fold.
183:184 binding

P18669 Phosphoglycerate mutase 1; BPG-dependent PGAM 1; Phosphoglycerate mutase isozyme B; PGAM-B; EC 5.4.2.11; EC 5.4.2.4 from Homo sapiens (Human) (see 4 papers)
33% identity, 54% coverage: 5:108/193 of query aligns to 1:109/254 of P18669

query
sites
P18669

M
|
M

A

A

Y

A

P

Y

K

K

I

L

W

V

F

L

L

I

R
|
R

H
|
H

G
|
G

Q
x
E

T
x
S

E
x
A

W
|
W

N
|
N

A

L

E

E

G

N

R

R

I

F

Q
x
S

G
|
G

Q

W

L
x
Y

E

D

S

A

R

D

L

L

S

S

P

P

L

A

G

G

I

H

E

E

H

E

A

A

Q

K

Q

R

Q

G

A

G

G

Q

L

A

M

L

A

R

P

D

I

A

L

G

A

Y

Q

E

G

F

P

D

A

I

C

C

Y

F

V

T

S

S

P

V

L

Q

G

K

R

R

A

A

Q

I

Q

R

T

T

A

L

R

W

I

T

A

V

L

L

G

D

-

A

-

I

D

D

Q

Q

-

M

-

W

-

L

P

P

F

V

I

V

T

R

D

T

A

W

R

R

L

L

A

N

E
|
E

A
x
R

Q
x
H

A
x
Y

G

G

V

G

F

L

Q

T

G

G

L

L

T

N

R
x
K

Q

A

E

E

V

T

A

A

E

K

Y

H

P

G

E

E

M1 (= M5) modified: Initiator methionine, Removed
10:17 (vs. 14:21, 63% identical) binding
H11 (= H15) active site, Tele-phosphohistidine intermediate
SG 23:24 (≠ QG 27:28) binding
Y26 (≠ L30) modified: Phosphotyrosine
ERHY 89:92 (≠ EAQA 88:91) binding
K100 (≠ R99) binding

Sites not aligning to the query:

116:117 binding
251 modified: N6-acetyllysine; alternate
253 modified: N6-acetyllysine
254 modified: N6-acetyllysine

P62707 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase; BPG-dependent PGAM; PGAM; Phosphoglyceromutase; dPGM; EC 5.4.2.11 from Escherichia coli (strain K12) (see 6 papers)
33% identity, 53% coverage: 5:106/193 of query aligns to 1:107/250 of P62707

query
sites
P62707

M
|
M

A

A

Y

V

P

T

K

K

I

L

W

V

F

L

L

V

R
|
R

H
|
H

G
|
G

Q
x
E

T
x
S

E
x
Q

W
|
W

N
|
N

A
x
K

E

E

G

N

R

R

I

F

Q
x
T

G
|
G

Q

W

L

Y

E

D

S

V

R

D

L

L

S

S

P

E

L

K

G

G

I

V

E

S

H

E

A

A

Q

K

Q

A

A

G

G

K

L

L

M

L

A

K

P

E

I

E

L

G

A

Y

Q

S

G

F

P

D

A

F

C

A

Y

Y

V

T

S

S

P

V

L

L

G

K

R

R

A

A

Q

I

Q

H

T

T

A

L

R

W

I

N

A

V

L

L

G

D

-

E

-

L

D

D

Q

Q

-

A

-

W

-

L

P

P

F

V

I

E

T

K

D

S

A

W

R

K

L

L

A

N

E
|
E

A
x
R

Q
x
H

A
x
Y

G

G

V

A

F

L

Q

Q

G

G

L

L

T

N

R
x
K

Q

A

E

E

V

T

A

A

A

E

E
x
K

Y

Y

M1 (= M5) modified: Initiator methionine, Removed
10:17 (vs. 14:21, 63% identical) binding
H11 (= H15) active site, Tele-phosphohistidine intermediate
K18 (≠ A22) modified: N6-acetyllysine
TG 23:24 (≠ QG 27:28) binding
E89 (= E88) active site, Proton donor/acceptor
ERHY 89:92 (≠ EAQA 88:91) binding
K100 (≠ R99) binding ; modified: N6-acetyllysine
K106 (≠ E105) modified: N6-acetyllysine

Sites not aligning to the query:

116:117 binding
184 Transition state stabilizer
185:186 binding

1bifA 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase bifunctional enzyme complexed with atp-g-s and phosphate (see paper)
29% identity, 74% coverage: 10:152/193 of query aligns to 215:354/432 of 1bifA

query
sites
1bifA

I

I

W

Y

F

L

L

C

R
|
R

H
|
H

G

G

Q

E

T

S

E

E

W

L

N
|
N

A

L

E

K

G

G

R

R

I

I

Q

G

G

G

Q

-

L

-

E

D

S

P

R

G

L

L

S

S

P

P

L

R

G

G

I

R

E

E

H

F

A

S

Q

K

Q

H

Q

L

A

A

G

Q

L

F

M

I

A

S

P

D

I

Q

L

N

A

I

Q

K

G

D

P

L

A

K

C

V

Y

F

V

T

S

S

P

Q

L

M

G

K

R
|
R

A

T

Q

I

Q

Q

T

T

A

A

R

E

I

-

A

A

L

L

G

S

D

-

Q

V

P

P

F

Y

I

E

T

Q

D

F

A

K

R

V

L

L

A

N

E
|
E

A

I

Q

D

A

A

G

G

V

V

F

C

Q

E

G

E

L

M

T

T

R
x
Y

Q

E

E

E

V

I

A

Q

A

D

E

H

Y

Y

P

P

E

L

I

E

Y

F

A

A

A

L

N
x
R

P

D

L

Q

N

D

L
x
K

D

Y

L

R

F

Y

C

-

A

R

A

Y

P

P

Q

K

G

G

E

E

G

S

F
x
Y

D

E

A

D

F

L

Q

V

A

Q

R

R

I

L

T

E

D

P

F

V

L

I

T

M

G

E

L

L

S

E

-

R

-

Q

E

E

P

N

T

V

V

L

V

V

V

I

A

C

H
|
H

active site: R219 (= R14), H220 (= H15), N226 (= N21), R269 (= R66), E289 (= E88), H354 (= H152)
binding phosphate ion: R219 (= R14), H220 (= H15), R269 (= R66), E289 (= E88), Y300 (≠ R99), R314 (≠ N113), K318 (≠ L117), Y329 (≠ F129), H354 (= H152)

Sites not aligning to the query:

binding phosphothiophosphoric acid-adenylate ester: 11, 12, 13, 14, 15, 16, 17, 131, 134, 136, 184, 210, 391
binding phosphate ion: 359

5y2iB Phosphoglycerate mutase 1 (pgam1) complexed with its inhibitor pgmi- 004a
32% identity, 52% coverage: 9:108/193 of query aligns to 3:107/233 of 5y2iB

query
sites
5y2iB

K

K

I

L

W

V

F

L

L

I

R

R

H
|
H

G

G

Q

E

T

S

E

A

W

W

N

N

A

L

E
|
E

G
x
N

R

R

I
x
F

Q

S

G

G

Q

W

L

Y

E

D

S

A

R

D

L

L

S

S

P

P

L

A

G

G

I

H

E

E

H

E

A

A

Q

K

Q

R

Q

G

A

G

G

Q

L

A

M

L

A

R

P

D

I

A

L

G

A

Y

Q

E

G

F

P

D

A

I

C

C

Y

F

V

T

S

S

P

V

L

Q

G

K

R
|
R

A

A

Q

I

Q

R

T

T

A

L

R

W

I

T

A

V

L

L

G

D

-

A

-

I

D

D

Q

Q

-

M

-

W

-

L

P

P

F

V

I

V

T

R

D

T

A

W

R

R

L

L

A

N

E
|
E

A
x
R

Q

H

A

Y

G

G

V

G

F
x
L

Q

T

G

G

L

L

T

N

R

K

Q

A

E

E

V

T

A

A

E

K

Y

H

P

G

E

E

active site: H9 (= H15), R60 (= R66), E87 (= E88)
binding 3,4-bis(oxidanyl)-9,10-bis(oxidanylidene)-~{N}-[4-(trifluoromethyl)phenyl]anthracene-2-sulfonamide: E17 (= E23), N18 (≠ G24), F20 (≠ I26), R88 (≠ A89), L93 (≠ F94)

Sites not aligning to the query:

6isnC Phosphoglycerate mutase 1 complexed with a small molecule inhibitor
32% identity, 52% coverage: 9:108/193 of query aligns to 4:108/234 of 6isnC

query
sites
6isnC

K

K

I

L

W

V

F

L

L

I

R

R

H

H

G

G

Q

E

T

S

E

A

W

W

N

N

A

L

E

E

G
x
N

R
|
R

I
x
F

Q

S

G

G

Q

W

L

Y

E

D

S

A

R

D

L

L

S

S

P

P

L

A

G

G

I

H

E

E

H

E

A

A

Q

K

Q

R

Q

G

A

G

G

Q

L

A

M

L

A

R

P

D

I

A

L

G

A

Y

Q

E

G

F

P

D

A

I

C

C

Y

F

V

T

S

S

P

V

L

Q

G

K

R

R

A

A

Q

I

Q

R

T

T

A

L

R

W

I

T

A

V

L

L

G

D

-

A

-

I

D

D

Q

Q

-

M

-

W

-

L

P

P

F

V

I

V

T

R

D

T

A

W

R

R

L

L

A

N

E

E

A
x
R

Q

H

A

Y

G

G

V

G

F

L

Q

T

G

G

L

L

T

N

R
x
K

Q

A

E

E

V

T

A

A

E

K

Y

H

P

G

E

E

binding 4-chloro-N-(3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)benzene-1-sulfonamide: N19 (≠ G24), R20 (= R25), F21 (≠ I26), R89 (≠ A89), K99 (≠ R99)

Sites not aligning to the query:

binding 4-chloro-N-(3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)benzene-1-sulfonamide: 115

5zrmC Phosphoglycerate mutase 1 complexed with a small molecule inhibitor in-ac
32% identity, 52% coverage: 9:108/193 of query aligns to 4:108/234 of 5zrmC

query
sites
5zrmC

K

K

I

L

W

V

F

L

L

I

R

R

H

H

G

G

Q

E

T

S

E

A

W

W

N

N

A

L

E

E

G
x
N

R

R

I
x
F

Q

S

G

G

Q

W

L

Y

E

D

S

A

R

D

L

L

S

S

P

P

L

A

G

G

I

H

E

E

H

E

A

A

Q

K

Q

R

Q

G

A

G

G

Q

L

A

M

L

A

R

P

D

I

A

L

G

A

Y

Q

E

G

F

P

D

A

I

C

C

Y

F

V

T

S

S

P

V

L

Q

G

K

R

R

A

A

Q

I

Q

R

T

T

A

L

R

W

I

T

A

V

L

L

G

D

-

A

-

I

D

D

Q

Q

-

M

-

W

-

L

P

P

F

V

I

V

T

R

D

T

A

W

R

R

L

L

A

N

E

E

A
x
R

Q

H

A

Y

G

G

V

G

F

L

Q

T

G

G

L

L

T

N

R
x
K

Q

A

E

E

V
x
T

A

A

E

K

Y

H

P

G

E

E

binding 2-[(3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)sulfamoyl]phenyl acetate: N19 (≠ G24), F21 (≠ I26), R89 (≠ A89), K99 (≠ R99), T102 (≠ V102)

Sites not aligning to the query:

binding 2-[(3,4-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl)sulfamoyl]phenyl acetate: 111, 114, 115

5y35C Phosphoglycerate mutase 1 complexed with a small molecule inhibitor kh1
32% identity, 52% coverage: 9:108/193 of query aligns to 4:108/237 of 5y35C

query
sites
5y35C

K

K

I

L

W

V

F

L

L

I

R

R

H
|
H

G

G

Q

E

T

S

E

A

W

W

N

N

A

L

E

E

G
x
N

R

R

I
x
F

Q

S

G

G

Q

W

L

Y

E

D

S

A

R

D

L

L

S

S

P

P

L

A

G

G

I

H

E

E

H

E

A

A

Q

K

Q

R

Q

G

A

G

G

Q

L

A

M

L

A

R

P

D

I

A

L

G

A

Y

Q

E

G

F

P

D

A

I

C

C

Y

F

V

T

S

S

P

V

L

Q

G

K

R
|
R

A

A

Q

I

Q

R

T

T

A

L

R

W

I

T

A

V

L

L

G

D

-

A

-

I

D

D

Q

Q

-

M

-

W

-

L

P

P

F

V

I

V

T

R

D

T

A

W

R

R

L

L

A

N

E
|
E

A
x
R

Q

H

A

Y

G

G

V

G

F

L

Q

T

G

G

L

L

T

N

R
x
K

Q

A

E

E

V

T

A

A

E

K

Y

H

P

G

E

E

active site: H10 (= H15), R61 (= R66), E88 (= E88)
binding ~{N}-[3,4-bis(oxidanyl)-9,10-bis(oxidanylidene)anthracen-2-yl]-4-methyl-benzenesulfonamide: N19 (≠ G24), F21 (≠ I26), R89 (≠ A89), K99 (≠ R99)

Sites not aligning to the query:

7xb9B Phosphoglycerate mutase 1 complexed with a covalent inhibitor
32% identity, 52% coverage: 9:108/193 of query aligns to 3:107/231 of 7xb9B

query
sites
7xb9B

K

K

I

L

W

V

F

L

L

I

R

R

H

H

G

G

Q

E

T

S

E

A

W

W

N

N

A

L

E

E

G

N

R

R

I
x
F

Q

S

G

G

Q

W

L

Y

E

D

S

A

R

D

L

L

S

S

P

P

L

A

G

G

I

H

E

E

H

E

A

A

Q

K

Q

R

Q

G

A

G

G

Q

L

A

M

L

A

R

P

D

I

A

L

G

A

Y

Q

E

G

F

P

D

A

I

C

C

Y

F

V

T

S

S

P

V

L

Q

G

K

R

R

A

A

Q

I

Q

R

T

T

A

L

R

W

I

T

A

V

L

L

G

D

-

A

-

I

D

D

Q

Q

-

M

-

W

-

L

P

P

F

V

I

V

T

R

D

T

A

W

R

R

L

L

A

N

E

E

A
x
R

Q

H

A
x
Y

G

G

V

G

F

L

Q

T

G

G

L

L

T

N

R
x
K

Q

A

E

E

V

T

A

A

E

K

Y

H

P

G

E

E

binding 3-[[5-(cyclopenten-1-yl)-2-methoxycarbonyl-phenyl]sulfamoyl]benzenesulfonic acid: F20 (≠ I26), R88 (≠ A89), Y90 (≠ A91), K98 (≠ R99)

Sites not aligning to the query:

binding 3-[[5-(cyclopenten-1-yl)-2-methoxycarbonyl-phenyl]sulfamoyl]benzenesulfonic acid: 113, 114, 186

Query Sequence

>GFF2008 FitnessBrowser__Phaeo:GFF2008
MRAGMAYPKIWFLRHGQTEWNAEGRIQGQLESRLSPLGIEHAQQQAGLMAPILAQGPACY
VSPLGRAQQTARIALGDQPFITDARLAEAQAGVFQGLTRQEVAAEYPEIYAANPLNLDLF
CAAPQGEGFDAFQARITDFLTGLSEPTVVVAHGLWGQVLRGVICGLSRAEMAALPNEQGC
IYQLADGGEQVLR

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SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory