SitesBLAST
Comparing GFF202 FitnessBrowser__WCS417:GFF202 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
54% identity, 90% coverage: 12:242/258 of query aligns to 9:239/240 of 4ymuJ
- binding adenosine-5'-triphosphate: F11 (= F14), V16 (= V19), S36 (= S39), G37 (= G40), S38 (≠ C41), G39 (= G42), K40 (= K43), S41 (= S44), T42 (= T45), E162 (= E165), H194 (= H197)
- binding magnesium ion: S41 (= S44), E162 (= E165)
3c4jA Abc protein artp in complex with atp-gamma-s
53% identity, 92% coverage: 4:241/258 of query aligns to 3:240/242 of 3c4jA
3c41J Abc protein artp in complex with amp-pnp/mg2+
53% identity, 92% coverage: 4:241/258 of query aligns to 3:240/242 of 3c41J
2olkA Abc protein artp in complex with adp-beta-s
53% identity, 92% coverage: 4:241/258 of query aligns to 3:240/242 of 2olkA
2oljA Abc protein artp in complex with adp/mg2+
53% identity, 92% coverage: 4:241/258 of query aligns to 3:240/242 of 2oljA
4u00A Crystal structure of ttha1159 in complex with adp (see paper)
53% identity, 92% coverage: 4:241/258 of query aligns to 2:238/241 of 4u00A
P02915 Histidine/lysine/arginine/ornithine transport ATP-binding protein HisP; EC 7.4.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
45% identity, 90% coverage: 10:240/258 of query aligns to 12:254/258 of P02915
- S41 (= S39) binding
- G42 (= G40) binding
- G44 (= G42) binding
- K45 (= K43) binding
- S46 (= S44) binding
- T47 (= T45) binding
1b0uA Atp-binding subunit of the histidine permease from salmonella typhimurium (see paper)
45% identity, 90% coverage: 10:240/258 of query aligns to 8:250/258 of 1b0uA
P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
37% identity, 92% coverage: 4:241/258 of query aligns to 1:243/343 of P30750
- 40:46 (vs. 39:45, 86% identical) binding
- E166 (= E165) mutation to Q: Exhibits little ATPase activity.
Sites not aligning to the query:
- 278:283 binding
- 295 N→A: Reduces the binding of L-methionine to undetectable levels.
- 295:296 binding
6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
37% identity, 92% coverage: 4:241/258 of query aligns to 2:244/344 of 6cvlD
- binding phosphothiophosphoric acid-adenylate ester: F12 (= F14), Q14 (≠ E16), I19 (vs. gap), S41 (= S39), G42 (= G40), A43 (≠ C41), G44 (= G42), K45 (= K43), S46 (= S44), T47 (= T45), N141 (≠ Q139), S143 (= S141), Q146 (= Q144), H200 (= H197)
3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
37% identity, 92% coverage: 4:241/258 of query aligns to 2:244/344 of 3tuzC
Sites not aligning to the query:
3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
37% identity, 92% coverage: 4:241/258 of query aligns to 2:244/344 of 3tuiC
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
35% identity, 98% coverage: 1:254/258 of query aligns to 14:264/378 of P69874
- C26 (≠ F13) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F14) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (≠ V32) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C41) mutation to T: Loss of ATPase activity and transport.
- L60 (= L47) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ F63) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V127) mutation to M: Loss of ATPase activity and transport.
- D172 (= D164) mutation to N: Loss of ATPase activity and transport.
Sites not aligning to the query:
- 276 C→A: Lower ATPase activity and transport efficiency.
- 297 mutation E->K,D: Lower ATPase activity and transport efficiency.; E→Q: Loss of ATPase activity and transport.
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
39% identity, 93% coverage: 3:242/258 of query aligns to 1:232/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F14), S37 (= S39), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), Q81 (= Q87), R128 (≠ A135), A132 (≠ Q139), S134 (= S141), G136 (= G143), Q137 (= Q144), E158 (= E165), H191 (= H197)
- binding magnesium ion: S42 (= S44), Q81 (= Q87)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
39% identity, 93% coverage: 3:242/258 of query aligns to 1:232/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F14), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (≠ A135), S134 (= S141), Q137 (= Q144)
- binding beryllium trifluoride ion: S37 (= S39), G38 (= G40), K41 (= K43), Q81 (= Q87), S134 (= S141), G136 (= G143), H191 (= H197)
- binding magnesium ion: S42 (= S44), Q81 (= Q87)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
39% identity, 93% coverage: 3:242/258 of query aligns to 1:232/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F14), V17 (= V19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (≠ A135), A132 (≠ Q139), S134 (= S141), Q137 (= Q144)
- binding tetrafluoroaluminate ion: S37 (= S39), G38 (= G40), K41 (= K43), Q81 (= Q87), S134 (= S141), G135 (= G142), G136 (= G143), E158 (= E165), H191 (= H197)
- binding magnesium ion: S42 (= S44), Q81 (= Q87)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
39% identity, 93% coverage: 3:242/258 of query aligns to 1:232/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F14), V17 (= V19), G38 (= G40), C39 (= C41), G40 (= G42), K41 (= K43), S42 (= S44), T43 (= T45), R128 (≠ A135), A132 (≠ Q139), S134 (= S141), Q137 (= Q144)
- binding magnesium ion: S42 (= S44), Q81 (= Q87)
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
39% identity, 93% coverage: 3:242/258 of query aligns to 1:232/374 of 2awnB
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
39% identity, 93% coverage: 3:242/258 of query aligns to 2:233/371 of P68187
- A85 (≠ H90) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ D112) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (≠ A120) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ L123) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (= E125) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A130) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G143) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D164) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ Q237) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 241 F→I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 267 W→G: Normal maltose transport but constitutive mal gene expression.
- 278 G→P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 282 S→L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 284 G→S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 302 G→D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 308 E→Q: Maltose transport is affected but retains ability to interact with MalT.
- 322 S→F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
38% identity, 92% coverage: 5:242/258 of query aligns to 1:230/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F14), S35 (= S39), G36 (= G40), C37 (= C41), G38 (= G42), K39 (= K43), S40 (= S44), T41 (= T45), R126 (≠ A135), A130 (≠ Q139), S132 (= S141), G134 (= G143), Q135 (= Q144)
Query Sequence
>GFF202 FitnessBrowser__WCS417:GFF202
MSALIEFQGFNKFFGEAQVLKGIDLSVQRGEVVVILGPSGCGKSTLLRCLNGLEVAHSGS
LRFAGNELLDKQTDWRQVRQDIGMVFQSYHLFPHMSVLDNILLGPLKVQQRDPREAREQA
EKLLERVGLAEKRDAFPRQLSGGQQQRIAIVRSLCMNPQVMLFDEVTAALDPEMVKEVLE
VIQGLARDGMTLLIVTHEMAFARAVADRVVFMEAGRILEQNTPEAFFTNPQTARAQQFLE
KFSFVSTLPKKTKELELI
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory