SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF205 HP15_204 oligopeptide/dipeptide ABC transporter, ATPase subunit to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q8RDH4 Dipeptide transport ATP-binding protein DppD; EC 7.4.2.9 from Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) (Thermoanaerobacter tengcongensis) (see paper)
34% identity, 90% coverage: 35:335/336 of query aligns to 17:323/326 of Q8RDH4

query
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Q8RDH4

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ASGKST 44:49 (≠ GCGKST 62:67) binding
N61 (≠ S79) binding
Q97 (= Q110) binding
C285 (= C297) binding
C291 (= C303) binding
C298 (= C310) binding
C316 (= C328) binding

4fwiB Crystal structure of the nucleotide-binding domain of a dipeptide abc transporter (see paper)
33% identity, 89% coverage: 35:333/336 of query aligns to 16:310/310 of 4fwiB

query
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4fwiB

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binding adenosine-5'-triphosphate: I20 (≠ V39), S42 (= S61), A43 (≠ G62), S44 (≠ C63), G45 (= G64), K46 (= K65), S47 (= S66), T48 (= T67), N60 (≠ S79), Q96 (= Q110), E179 (= E192), L267 (≠ P290)
binding magnesium ion: S47 (= S66), Q96 (= Q110), D178 (= D191)
binding iron/sulfur cluster: H247 (= H260), P248 (= P261), C274 (= C297), H277 (= H300), C280 (= C303), Y282 (= Y305), A283 (= A306), C287 (= C310), P292 (= P315), C305 (= C328), H306 (= H329)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 13

P0AAH4 Putrescine export system ATP-binding protein SapD from Escherichia coli (strain K12) (see paper)
34% identity, 83% coverage: 32:310/336 of query aligns to 13:305/330 of P0AAH4

query
sites
P0AAH4

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K46 (= K65) mutation K->G,I,Q,R: 25% to 50% stimulation of K(+) uptake by TrkH.; mutation Missing: Almost no stimulation of K(+) uptake by TrkH.
G162 (= G170) mutation to A: 80% stimulation of K(+) uptake by TrkH.
Q165 (= Q173) mutation to L: 60% stimulation of K(+) uptake by TrkH.
D183 (= D191) mutation to E: About 60% stimulation of K(+) uptake by TrkH.; mutation D->K,N: 15% to 20% stimulation of K(+) uptake by TrkH.

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
34% identity, 76% coverage: 32:286/336 of query aligns to 11:260/343 of P30750

query
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P30750

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40:46 (vs. 61:67, 86% identical) binding
E166 (= E192) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
34% identity, 76% coverage: 32:286/336 of query aligns to 12:261/344 of 3tuzC

query
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3tuzC

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Q

P

K

A

G

G

E

Q

A

I

L

Y

C

G

V

V

V

I

G

G

E

A

S

S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

I

R

R

T

C

V

V

M

N

G

L

L

L

L

E

S

R

P

P

S

T

A

E

G

G

E

S

I

V

H

L

Y

V

D

D

G

G

Q

Q

R

E

I

L

D

T

N

T

L

L

E

S

R

E

K

S

D

E

S

L

L

T

P

K

Y

A

R

R

K

Q

M

I

Q

G

M

M

I

I

F

F

Q

Q

N

H

P

-

Y

F

A

N

S

L

L

L

N

S

P

S

R

R

M

-

T

T

I

V

Q

F

Q

G

T

N

L

V

E

A

E

L

P

P

I

L

R

E

F

L

H

-

P

-

D

D

W

N

S

T

P

P

V

K

-

D

Q

E

V

V

R

K

D

R

K

R

I

V

H

T

E

E

V

L

M

L

H

S

S

L

V

V

G

G

I

L

D

G

Q

-

D

D

W

K

G

H

N

D

R

S

F

Y

G

P

H

S

E

N

F

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

I

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

V

S

D

N

P

P

E

K

F

V

I

L

V

L

A

C

D

D

E

Q

P

A

I

T

S

S

A

A

L

L

D

D

V

P

S

A

I

T

Q

T

A

R

Q

S

V

I

L

L

N

E

L

L

M

K

D

D

A

I

Q

N

E

R

S

R

R

L

G

G

L

L

T

T

Y

I

L

L

F

L

I

I

T

T

H

H

D

E

L

M

A

D

V

V

E

K

H

R

F

I

G

C

T

D

R

C

V

V

A

A

V

V

M

I

Y

S

L

N

G

G

R

E

V

L

C

I

E

E

L

Q

A

D

D

T

T

V

K

S

T

E

L

V

F

F

S

S

A

H

P

P

R

K

H

T

P

P

Y

L

T

A

Q

Q

A

K

L

F

L

I

S

Q

A

S

I

T

P

L

K

H

L

L

E

D

D

I

D

P

R

E

P

D

N

Y

H

Q

I

E

R

R

L

L

K

Q

G

A

E

E

binding adenosine-5'-diphosphate: F12 (= F32), Q14 (≠ R34), A21 (= A41), G42 (= G62), A43 (≠ C63), G44 (= G64), K45 (= K65), S46 (= S66), T47 (= T67)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 312, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
34% identity, 76% coverage: 32:286/336 of query aligns to 12:261/344 of 3tuiC

query
sites
3tuiC

F
|
F

R

H

R
x
Q

K

G

Q

T

E

R

A

T

V

I

H

Q

A

A

I

L

N

N

G

N

V

V

D

S

L

L

E

H

V

V

Q

P

K

A

G

G

E

Q

A

I

L

Y

C

G

V

V

V

I

G

G

E

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

I

R

R

T

C

V

V

M

N

G

L

L

L

L

E

S

R

P

P

S

T

A

E

G

G

E

S

I

V

H

L

Y

V

D

D

G

G

Q

Q

R

E

I

L

D

T

N

T

L

L

E

S

R

E

K

S

D

E

S

L

L

T

P

K

Y

A

R

R

K

Q

M

I

Q

G

M

M

I

I

F

F

Q

Q

N

H

P

-

Y

F

A

N

S

L

L

L

N

S

P

S

R

R

M

-

T

T

I

V

Q

F

Q

G

T

N

L

V

E

A

E

L

P

P

I

L

R

E

F

L

H

-

P

-

D

D

W

N

S

T

P

P

V

K

-

D

Q

E

V

V

R

K

D

R

K

R

I

V

H

T

E

E

V

L

M

L

H

S

S

L

V

V

G

G

I

L

D

G

Q

-

D

D

W

K

G

H

N

D

R

S

F

Y

G

P

H

S

E

N

F

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

I

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

V

S

D

N

P

P

E

K

F

V

I

L

V

L

A

C

D

D

E

Q

P

A

I

T

S

S

A

A

L

L

D

D

V

P

S

A

I

T

Q

T

A

R

Q

S

V

I

L

L

N

E

L

L

M

K

D

D

A

I

Q

N

E

R

S

R

R

L

G

G

L

L

T

T

Y

I

L

L

F

L

I

I

T

T

H

H

D

E

L

M

A

D

V

V

E

K

H

R

F

I

G

C

T

D

R

C

V

V

A

A

V

V

M

I

Y

S

L

N

G

G

R

E

V

L

C

I

E

E

L

Q

A

D

D

T

T

V

K

S

T

E

L

V

F

F

S

S

A

H

P

P

R

K

H

T

P

P

Y

L

T

A

Q

Q

A

K

L

F

L

I

S

Q

A

S

I

T

P

L

K

H

L

L

E

D

D

I

D

P

R

E

P

D

N

Y

H

Q

I

E

R

R

L

L

K

Q

G

A

E

E

binding adenosine-5'-diphosphate: F12 (= F32), Q14 (≠ R34), S41 (= S61), G42 (= G62), A43 (≠ C63), G44 (= G64), K45 (= K65), S46 (= S66), T47 (= T67)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
34% identity, 76% coverage: 32:286/336 of query aligns to 12:261/344 of 6cvlD

query
sites
6cvlD

F
|
F

R

H

R
x
Q

K

G

Q

T

E

R

A

T

V
x
I

H

Q

A

A

I

L

N

N

G

N

V

V

D

S

L

L

E

H

V

V

Q

P

K

A

G

G

E

Q

A

I

L

Y

C

G

V

V

V

I

G

G

E

A

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

I

R

R

T

C

V

V

M

N

G

L

L

L

L

E

S

R

P

P

S

T

A

E

G

G

E

S

I

V

H

L

Y

V

D

D

G

G

Q

Q

R

E

I

L

D

T

N

T

L

L

E

S

R

E

K

S

D

E

S

L

L

T

P

K

Y

A

R

R

K

Q

M

I

Q

G

M

M

I

I

F

F

Q

Q

N

H

P

-

Y

F

A

N

S

L

L

L

N

S

P

S

R

R

M

-

T

T

I

V

Q

F

Q

G

T

N

L

V

E

A

E

L

P

P

I

L

R

E

F

L

H

-

P

-

D

D

W

N

S

T

P

P

V

K

-

D

Q

E

V

V

R

K

D

R

K

R

I

V

H

T

E

E

V

L

M

L

H

S

S

L

V

V

G

G

I

L

D

G

Q

-

D

D

W

K

G

H

N

D

R

S

F

Y

G

P

H

S

E
x
N

F
x
L

S
|
S

G
|
G

Q
|
Q

R

K

Q

Q

R

R

I

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

V

S

D

N

P

P

E

K

F

V

I

L

V

L

A

C

D

D

E

Q

P

A

I

T

S

S

A

A

L

L

D

D

V

P

S

A

I

T

Q

T

A

R

Q

S

V

I

L

L

N

E

L

L

M

K

D

D

A

I

Q

N

E

R

S

R

R

L

G

G

L

L

T

T

Y

I

L

L

F

L

I

I

T

T

H
|
H

D

E

L

M

A

D

V

V

E

K

H

R

F

I

G

C

T

D

R

C

V

V

A

A

V

V

M

I

Y

S

L

N

G

G

R

E

V

L

C

I

E

E

L

Q

A

D

D

T

T

V

K

S

T

E

L

V

F

F

S

S

A

H

P

P

R

K

H

T

P

P

Y

L

T

A

Q

Q

A

K

L

F

L

I

S

Q

A

S

I

T

P

L

K

H

L

L

E

D

D

I

D

P

R

E

P

D

N

Y

H

Q

I

E

R

R

L

L

K

Q

G

A

E

E

binding phosphothiophosphoric acid-adenylate ester: F12 (= F32), Q14 (≠ R34), I19 (≠ V39), S41 (= S61), G42 (= G62), A43 (≠ C63), G44 (= G64), K45 (= K65), S46 (= S66), T47 (= T67), N141 (≠ E166), L142 (≠ F167), S143 (= S168), G144 (= G169), G145 (= G170), Q146 (= Q171), H200 (= H225)

1oxvD Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 80% coverage: 35:304/336 of query aligns to 14:278/353 of 1oxvD

query
sites
1oxvD

K

K

Q

K

E

G

A

K

V
|
V

H

V

A
|
A

I

L

N

D

G

N

V

V

D

N

L

I

E

N

V

I

Q

E

K

N

G

G

E

E

A

R

L

F

C

G

V

I

V

L

G

G

E
x
P

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
x
T

T
|
T

V

F

A

M

R

R

T

I

V

I

M

A

G

G

L

L

L

D

S

V

P

P

S

S

A

T

G

G

E

E

I

L

H

Y

Y

F

D

D

G

D

Q

R

R

L

I

V

D

A

N

S

L

N

E

G

R

K

K

L

D

I

S

V

L

P

P

P

Y

E

R

D

R

R

K

K

M

I

Q

G

M

M

I

V

F

F

Q
|
Q

N

T

P

-

Y

-

A

W

S

A

L

L

N

Y

P

P

R

N

M

L

T

T

I

A

Q

F

Q

E

T

N

L

I

E

A

E

F

P

P

I

L

R

-

F

-

H

T

H

N

P

M

D

K

W

M

S

S

P

K

V

E

Q

E

V

I

R

R

D

K

K

R

I

V

H

E

E

E

V

V

M

A

H

K

S

I

V

L

G

D

I

I

D

H

Q

H

D

V

W

L

G

-

N

N

R

H

F

F

G

P

H

R

E

E

F

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

I

V

A

A

I

L

A

A

R

R

A

A

L

L

A

V

V

K

D

D

P

P

E

S

F

L

I

L

V

L

A

L

D

D

E
|
E

P

P

I

F

S

S

A

N

L

L

D

D

V

A

S

R

I

M

Q

R

A

D

Q

S

V

A

L

R

N

A

L

L

L

V

M

K

D

E

A

V

Q

Q

E

S

S

R

R

L

G

G

L

V

T

T

Y

L

L

L

F

V

I

V

T

S

H

H

D

D

L

P

A

A

V

D

V

I

E

F

H

A

F

I

G

A

T

D

R

R

V

V

A

G

V

V

M

L

Y

V

L

K

G

G

R

K

V

L

C

V

E

Q

L

V

A

G

D

K

T

P

K

E

T

D

L

L

F

Y

S

D

A

N

P

P

R

V

H

S

P

I

Y

Q

T

V

Q

A

A

S

L

L

L

I

S

G

A

E

I

I

P

N

K

E

L

L

E

E

D

G

D

K

R

V

P

T

N

N

H

E

I

G

R

V

L

V

K

I

G

G

E

S

V

L

P

R

T

F

P

P

V

V

N

S

L

V

P

S

S

S

G

D

C

R

V

A

F

I

H

I

G

G

R

I

C

R

P

P

binding phosphoaminophosphonic acid-adenylate ester: V18 (= V39), A20 (= A41), P39 (≠ E60), S40 (= S61), G41 (= G62), A42 (≠ C63), G43 (= G64), K44 (= K65), T45 (≠ S66), T46 (= T67), Q89 (= Q110), E166 (= E192)
binding magnesium ion: T45 (≠ S66), Q89 (= Q110)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxvA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 80% coverage: 35:304/336 of query aligns to 14:278/353 of 1oxvA

query
sites
1oxvA

K

K

Q

K

E

G

A

K

V
|
V

H

V

A
|
A

I

L

N

D

G

N

V

V

D

N

L

I

E

N

V

I

Q

E

K

N

G

G

E

E

A

R

L

F

C

G

V

I

V

L

G

G

E
x
P

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
x
T

T
|
T

V

F

A

M

R

R

T

I

V

I

M

A

G

G

L

L

L

D

S

V

P

P

S

S

A

T

G

G

E

E

I

L

H

Y

Y

F

D

D

G

D

Q

R

R

L

I

V

D

A

N

S

L

N

E

G

R

K

K

L

D

I

S

V

L

P

P

P

Y

E

R

D

R

R

K

K

M

I

Q

G

M

M

I

V

F

F

Q

Q

N

T

P

-

Y

-

A

W

S

A

L

L

N

Y

P

P

R

N

M

L

T

T

I

A

Q

F

Q

E

T

N

L

I

E

A

E

F

P

P

I

L

R

-

F

-

H

T

H

N

P

M

D

K

W

M

S

S

P

K

V

E

Q

E

V

I

R

R

D

K

K

R

I

V

H

E

E

E

V

V

M

A

H

K

S

I

V

L

G

D

I

I

D

H

Q

H

D

V

W

L

G

-

N

N

R

H

F

F

G

P

H

R

E

E

F

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

I

V

A

A

I

L

A

A

R

R

A

A

L

L

A

V

V

K

D

D

P

P

E

S

F

L

I

L

V

L

A

L

D

D

E
|
E

P

P

I

F

S

S

A

N

L

L

D

D

V

A

S

R

I

M

Q

R

A

D

Q

S

V

A

L

R

N

A

L

L

L

V

M

K

D

E

A

V

Q

Q

E

S

S

R

R

L

G

G

L

V

T

T

Y

L

L

L

F

V

I

V

T

S

H

H

D

D

L

P

A

A

V

D

V

I

E

F

H

A

F

I

G

A

T

D

R

R

V

V

A

G

V

V

M

L

Y

V

L

K

G

G

R

K

V

L

C

V

E

Q

L

V

A

G

D

K

T

P

K

E

T

D

L

L

F

Y

S

D

A

N

P

P

R

V

H

S

P

I

Y

Q

T

V

Q

A

A

S

L

L

L

I

S

G

A

E

I

I

P

N

K

E

L

L

E

E

D

G

D

K

R

V

P

T

N

N

H

E

I

G

R

V

L

V

K

I

G

G

E

S

V

L

P

R

T

F

P

P

V

V

N

S

L

V

P

S

S

S

G

D

C

R

V

A

F

I

H

I

G

G

R

I

C

R

P

P

binding phosphoaminophosphonic acid-adenylate ester: V18 (= V39), A20 (= A41), P39 (≠ E60), S40 (= S61), G41 (= G62), A42 (≠ C63), G43 (= G64), K44 (= K65), T45 (≠ S66), T46 (= T67), E166 (= E192)

Sites not aligning to the query:

binding phosphoaminophosphonic acid-adenylate ester: 13

1oxuA Crystal structure of glcv, the abc-atpase of the glucose abc transporter from sulfolobus solfataricus (see paper)
30% identity, 80% coverage: 35:304/336 of query aligns to 14:278/353 of 1oxuA

query
sites
1oxuA

K

K

Q

K

E

G

A

K

V

V

H

V

A
|
A

I

L

N

D

G

N

V

V

D

N

L

I

E

N

V

I

Q

E

K

N

G

G

E

E

A

R

L

F

C

G

V

I

V

L

G

G

E

P

S
|
S

G
|
G

C
x
A

G
|
G

K
|
K

S
x
T

T
|
T

V

F

A

M

R

R

T

I

V

I

M

A

G

G

L

L

L

D

S

V

P

P

S

S

A

T

G

G

E

E

I

L

H

Y

Y

F

D

D

G

D

Q

R

R

L

I

V

D

A

N

S

L

N

E

G

R

K

K

L

D

I

S

V

L

P

P

P

Y

E

R

D

R

R

K

K

M

I

Q

G

M

M

I

V

F

F

Q

Q

N

T

P

-

Y

-

A

W

S

A

L

L

N

Y

P

P

R

N

M

L

T

T

I

A

Q

F

Q

E

T

N

L

I

E

A

E

F

P

P

I

L

R

-

F

-

H

T

H

N

P

M

D

K

W

M

S

S

P

K

V

E

Q

E

V

I

R

R

D

K

K

R

I

V

H

E

E

E

V

V

M

A

H

K

S

I

V

L

G

D

I

I

D

H

Q

H

D

V

W

L

G

-

N

N

R

H

F

F

G

P

H

R

E

E

F

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

I

V

A

A

I

L

A

A

R

R

A

A

L

L

A

V

V

K

D

D

P

P

E

S

F

L

I

L

V

L

A

L

D

D

E

E

P

P

I

F

S

S

A

N

L

L

D

D

V

A

S

R

I

M

Q

R

A

D

Q

S

V

A

L

R

N

A

L

L

L

V

M

K

D

E

A

V

Q

Q

E

S

S

R

R

L

G

G

L

V

T

T

Y

L

L

L

F

V

I

V

T

S

H

H

D

D

L

P

A

A

V

D

V

I

E

F

H

A

F

I

G

A

T

D

R

R

V

V

A

G

V

V

M

L

Y

V

L

K

G

G

R

K

V

L

C

V

E

Q

L

V

A

G

D

K

T

P

K

E

T

D

L

L

F

Y

S

D

A

N

P

P

R

V

H

S

P

I

Y

Q

T

V

Q

A

A

S

L

L

L

I

S

G

A

E

I

I

P

N

K

E

L

L

E

E

D

G

D

K

R

V

P

T

N

N

H

E

I

G

R

V

L

V

K

I

G

G

E

S

V

L

P

R

T

F

P

P

V

V

N

S

L

V

P

S

S

S

G

D

C

R

V

A

F

I

H

I

G

G

R

I

C

R

P

P

binding adenosine-5'-diphosphate: A20 (= A41), S40 (= S61), G41 (= G62), A42 (≠ C63), G43 (= G64), K44 (= K65), T45 (≠ S66), T46 (= T67)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 13

Q97UY8 Glucose import ATP-binding protein GlcV; EC 7.5.2.- from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
30% identity, 80% coverage: 35:304/336 of query aligns to 14:278/353 of Q97UY8

query
sites
Q97UY8

K

K

Q

K

E

G

A

K

V

V

H

V

A

A

I

L

N

D

G

N

V

V

D

N

L

I

E

N

V

I

Q

E

K

N

G

G

E

E

A

R

L

F

C

G

V

I

V

L

G

G

E

P

S

S

G

G

C

A

G

G

K

K

S

T

T

T

V

F

A

M

R

R

T

I

V

I

M

A

G

G

L

L

L

D

S

V

P

P

S

S

A

T

G

G

E

E

I

L

H

Y

Y

F

D

D

G

D

Q

R

R

L

I

V

D

A

N

S

L

N

E

G

R

K

K

L

D

I

S

V

L

P

P

P

Y

E

R

D

R

R

K

K

M

I

Q

G

M

M

I

V

F

F

Q

Q

N

T

P

-

Y

-

A

W

S

A

L

L

N

Y

P

P

R

N

M

L

T

T

I

A

Q

F

Q

E

T

N

L

I

E

A

E

F

P

P

I

L

R

-

F

-

H

T

H

N

P

M

D

K

W

M

S

S

P

K

V

E

Q

E

V

I

R

R

D

K

K

R

I

V

H

E

E

E

V

V

M

A

H

K

S

I

V

L

G

D

I

I

D

H

Q

H

D

V

W

L

G

-

N

N

R

H

F

F

G

P

H

R

E

E

F

L

S
|
S

G

G

G
|
G

Q

Q

R

Q

Q

Q

R

R

I

V

A

A

I

L

A

A

R

R

A

A

L

L

A

V

V

K

D

D

P

P

E

S

F

L

I

L

V

L

A

L

D

D

E
|
E

P

P

I

F

S

S

A

N

L

L

D

D

V

A

S

R

I

M

Q

R

A

D

Q

S

V

A

L

R

N

A

L

L

L

V

M

K

D

E

A

V

Q

Q

E

S

S

R

R

L

G

G

L

V

T

T

Y

L

L

L

F

V

I

V

T

S

H

H

D

D

L

P

A

A

V

D

V

I

E

F

H

A

F

I

G

A

T

D

R

R

V

V

A

G

V

V

M

L

Y

V

L

K

G

G

R

K

V

L

C

V

E

Q

L

V

A

G

D

K

T

P

K

E

T

D

L

L

F

Y

S

D

A

N

P

P

R

V

H

S

P

I

Y

Q

T

V

Q

A

A

S

L

L

L

I

S

G

A

E

I

I

P

N

K

E

L

L

E

E

D

G

D

K

R

V

P

T

N

N

H

E

I

G

R

V

L

V

K

I

G

G

E

S

V

L

P

R

T

F

P

P

V

V

N

S

L

V

P

S

S

S

G

D

C

R

V

A

F

I

H

I

G

G

R

I

C

R

P

P

S142 (= S168) mutation to A: Decrease in ATPase activity. Can form dimers.
G144 (= G170) mutation to A: Loss of ATPase activity. Cannot form dimers. Forms an active heterodimer; when associated with A-166.
E166 (= E192) mutation to A: Loss of ATPase activity. Can form dimers in the presence of ATP-Mg(2+). Forms an active heterodimer; when associated with A-144.; mutation to Q: Strong decrease in ATPase activity. Can form dimers in the presence of ATP alone, without Mg(2+).

4u00A Crystal structure of ttha1159 in complex with adp (see paper)
33% identity, 69% coverage: 39:270/336 of query aligns to 15:239/241 of 4u00A

query
sites
4u00A

V
x
L

H

H

A
x
V

I

L

N

K

G

G

V

I

D

H

L

L

E

E

V

V

Q

A

K

P

G

G

E

E

A

K

L

L

C

V

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

I

R

R

T

T

V

I

M

N

G

R

L

L

L

E

S

D

P

F

S

Q

A

E

G

G

E

E

I

V

H

V

Y

V

D

D

G

G

Q

L

R

S

I

V

D

K

N

D

L

-

E

-

R

D

K

R

D

A

S

L

L

R

P

E

Y

I

R

R

K

E

M

V

Q

G

M

M

I

V

F

F

Q

Q

N

Q

P

-

Y

-

A

F

S

N

L

L

N

F

P

P

R

H

M

M

T

T

I

V

Q

L

Q

E

T

N

L

V

E

T

-

L

E

A

P

P

I

M

R

R

F

V

H

R

P

-

D

-

W

W

S

P

P

R

V

E

Q

K

V

A

R

E

D

K

K

K

I

A

H

L

E

E

V

L

M

L

H

E

S

R

V

V

G

G

I

I

D

-

Q

L

D

D

W

Q

G

A

N

R

R

K

F

Y

G

P

H

A

E

Q

F

L

S

S

G

G

Q

Q

R

Q

Q

Q

R

R

I

V

A

A

I

I

A

A

R

R

A

A

L

L

A

A

V

M

D

E

P

P

E

K

F

I

I

M

V

L

A

F

D

D

E

E

P

P

I

T

S

S

A

A

L

L

D

D

V

P

S

E

I

M

Q

V

A

G

Q

E

V

V

L

L

N

D

L

V

L

M

M

R

D

D

A

L

Q

A

E

Q

S

G

R

-

G

G

L

M

T

T

Y

M

L

V

F

V

I

V

T

T

H

H

D

E

L

M

A

G

V

F

V

A

E

R

H

E

F

V

G

A

T

D

R

R

V

V

A

V

V

F

M

M

Y

D

L

G

G

G

R

Q

V

I

C

V

E

E

L

E

A

G

D

R

T

P

K

E

T

E

L

I

F

F

S

T

A

R

P

P

R

K

H

E

P

E

Y

R

T

T

Q

R

A

S

L

F

L

L

S

Q

A

R

I

V

binding adenosine-5'-diphosphate: L15 (≠ V39), V17 (≠ A41), S37 (= S61), G38 (= G62), S39 (≠ C63), G40 (= G64), K41 (= K65), S42 (= S66), T43 (= T67)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 12

7ahhC Opua inhibited inward-facing, sbd docked (see paper)
30% identity, 87% coverage: 34:324/336 of query aligns to 34:337/382 of 7ahhC

query
sites
7ahhC

R

K

K

K

Q

T

E

G

A

A

V
x
T

H
x
V

A
x
G

I

V

N

Y

G

D

V

T

D

N

L

F

E

E

V

I

Q

N

K

E

G

G

E

E

A

I

L

F

C

V

V

I

V

M

G

G

E

L

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

L

R

R

T

L

V

L

M

N

G

R

L

L

L

I

S

E

P

P

S

T

A

S

G

G

E

K

I

I

H

F

Y

I

D

D

G

N

Q

Q

R

D

I

V

D

A

N

T

L

L

E

N

R

K

K

E

D

D

S

L

L

L

P

Q

Y

V

R

R

K

K

-

T

M

M

Q

S

M

M

I

V

F

F

Q

Q

N

N

P

-

Y

-

A

F

S

G

L

L

N

F

P

P

R

H

M

R

T

T

I

I

Q

L

Q

E

T

N

L

T

E

E

E

Y

P

G

I

L

R

E

F

V

H

Q

H

N

P

-

D

-

W

-

S

V

P

P

V

K

Q

E

V

E

R

R

D

R

K

K

I

R

H

A

E

E

V

K

M

A

H

L

S

D

V

N

G

A

I

N

D

L

Q

L

D

D

W

F

G

K

N

D

R

Q

F

Y

G

P

H

K

E

Q

F

L

S

S

G

G

Q

M

R

Q

Q

Q

R

R

I

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

V

N

D

D

P

P

E

E

F

I

I

L

V

L

A

M

D
|
D

E
|
E

P

A

I

F

S

S

A

A

L

L

D

D

V

P

S

L

I

I

Q

R

A

R

Q

E

V

M

L

Q

N

D

L

E

L

L

M

L

D

E

A

L

Q

Q

E

A

S

K

R

F

G

Q

L

K

T

T

Y

I

L

I

F

F

I

V

T

S

H

H

D

D

L

L

A

N

V

E

V

A

E

L

H

R

F

I

G

G

T

D

R

R

V

I

A

A

V

I

M

M

Y

K

L

D

G

G

R

K

V

I

C

M

E

Q

L

I

A

G

D

T

T

G

K

E

T

E

L

I

F

L

S

T

A

N

P

P

R

A

H

N

P

D

Y

Y

T

V

Q

K

A

T

L

F

L

V

S

E

-

D

-

T

-

A

-

E

-

N

-

I

A

M

I
|
I

P
|
P

K

A

L
|
L

-

T

-

N

-

I

E

D

D

V

D

D

R

G

P

P

N

S

H

-

I

V

R

A

L

L

K

K

G

K

-

M

-

K

-

T

-

E

E
|
E

V
|
V

P
x
S

T
x
S

P

L

V

M

N

A

L

V

P

D

S

K

G

K

C

R

V

Q

F

F

H

R

G

G

R

V

C

V

-

T

-

S

-

E

-

Q

P

A

Y

I

A

A

N

A

E

R

R

K

C

N

R

N

Q

Q

E

P

L

L

P

K

Q

D

L

V

I

M

T

T

D

D

G

V

G

G

T

T

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: I272 (= I270), P273 (= P271), L275 (= L273), E295 (= E286), V296 (= V287), S297 (≠ P288), S298 (≠ T289)
binding phosphoaminophosphonic acid-adenylate ester: T39 (≠ V39), V40 (≠ H40), G41 (≠ A41), S61 (= S61), G62 (= G62), S63 (≠ C63), G64 (= G64), K65 (= K65), S66 (= S66), T67 (= T67), D187 (= D191), E188 (= E192)

Sites not aligning to the query:

7aheC Opua inhibited inward facing (see paper)
30% identity, 87% coverage: 34:324/336 of query aligns to 34:337/382 of 7aheC

query
sites
7aheC

R

K

K

K

Q

T

E

G

A

A

V

T

H

V

A

G

I

V

N

Y

G

D

V

T

D

N

L

F

E

E

V

I

Q

N

K

E

G

G

E

E

A

I

L

F

C

V

V

I

V

M

G

G

E

L

S

S

G

G

C

S

G

G

K

K

S

S

T

T

V

L

A

L

R

R

T

L

V

L

M

N

G

R

L

L

L

I

S

E

P

P

S

T

A

S

G

G

E

K

I

I

H

F

Y

I

D

D

G

N

Q

Q

R

D

I

V

D

A

N

T

L

L

E

N

R

K

K

E

D

D

S

L

L

L

P

Q

Y

V

R

R

K

K

-

T

M

M

Q

S

M

M

I

V

F

F

Q

Q

N

N

P

-

Y

-

A

F

S

G

L

L

N

F

P

P

R

H

M

R

T

T

I

I

Q

L

Q

E

T

N

L

T

E

E

E

Y

P

G

I

L

R

E

F

V

H

Q

H

N

P

-

D

-

W

-

S

V

P

P

V

K

Q

E

V

E

R

R

D

R

K

K

I

R

H

A

E

E

V

K

M

A

H

L

S

D

V

N

G

A

I

N

D

L

Q

L

D

D

W

F

G

K

N

D

R

Q

F

Y

G

P

H

K

E

Q

F

L

S

S

G

G

Q

M

R

Q

Q

Q

R

R

I

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

V

N

D

D

P

P

E

E

F

I

I

L

V

L

A

M

D

D

E

E

P

A

I

F

S

S

A

A

L

L

D

D

V

P

S

L

I

I

Q

R

A

R

Q

E

V

M

L

Q

N

D

L

E

L

L

M

L

D

E

A

L

Q

Q

E

A

S

K

R

F

G

Q

L

K

T

T

Y

I

L

I

F

F

I

V

T

S

H

H

D

D

L

L

A

N

V

E

V

A

E

L

H

R

F

I

G

G

T

D

R

R

V

I

A

A

V

I

M

M

Y

K

L

D

G

G

R

K

V

I

C

M

E

Q

L

I

A

G

D

T

T

G

K

E

T

E

L

I

F

L

S

T

A

N

P

P

R

A

H

N

P

D

Y

Y

T

V

Q

K

A

T

L

F

L

V

S

E

-

D

-

T

-

A

-

E

-

N

-

I

A

M

I

I

P

P

K

A

L
|
L

-

T

-

N

-

I

E

D

D

V

D

D

R

G

P

P

N

S

H

-

I

V

R

A

L

L

K

K

G

K

-

M

-

K

-

T

-

E

E
|
E

V
|
V

P
x
S

T
x
S

P

L

V

M

N

A

L

V

P

D

S

K

G

K

C

R

V

Q

F

F

H

R

G

G

R

V

C

V

-

T

-

S

-

E

-

Q

P

A

Y

I

A

A

N

A

E

R

R

K

C

N

R

N

Q

Q

E

P

L

L

P

K

Q

D

L

V

I

M

T

T

D

D

G

V

G

G

T

T

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: L275 (= L273), E295 (= E286), V296 (= V287), S297 (≠ P288), S298 (≠ T289)

Sites not aligning to the query:

binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 357, 372

7ahdC Opua (e190q) occluded (see paper)
31% identity, 69% coverage: 34:264/336 of query aligns to 34:260/260 of 7ahdC

query
sites
7ahdC

R

K

K

K

Q

T

E

G

A

A

V
x
T

H

V

A

G

I

V

N

Y

G

D

V

T

D

N

L

F

E

E

V

I

Q

N

K

E

G

G

E

E

A

I

L

F

C

V

V

I

V

M

G

G

E

L

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

L

R

R

T

L

V

L

M

N

G

R

L

L

L

I

S

E

P

P

S

T

A

S

G

G

E

K

I

I

H

F

Y

I

D

D

G

N

Q

Q

R

D

I

V

D

A

N

T

L

L

E

N

R

K

K

E

D

D

S

L

L

L

P

Q

Y

V

R

R

K

K

-

T

M

M

Q

S

M

M

I

V

F

F

Q
|
Q

N

N

P

-

Y

-

A

F

S

G

L

L

N

F

P

P

R

H

M

R

T

T

I

I

Q

L

Q

E

T

N

L

T

E

E

E

Y

P

G

I

L

R

E

F

V

H

Q

H

N

P

-

D

-

W

-

S

V

P

P

V

K

Q

E

V

E

R

R

D

R

K

K

I

R

H

A

E

E

V

K

M

A

H

L

S

D

V

N

G

A

I

N

D

L

Q

L

D

D

W

F

G

K

N

D

R

Q

F

Y

G

P

H
x
K

E
x
Q

F

L

S
|
S

G

G

G
|
G

Q
x
M

R

Q

Q

Q

R

R

I

V

A

G

I

L

A

A

R

R

A

A

L

L

A

A

V

N

D

D

P

P

E

E

F

I

I

L

V

L

A

M

D

D

E
x
Q

P

A

I

F

S

S

A

A

L

L

D

D

V

P

S

L

I

I

Q

R

A

R

Q

E

V

M

L

Q

N

D

L

E

L

L

M

L

D

E

A

L

Q

Q

E

A

S

K

R

F

G

Q

L

K

T

T

Y

I

L

I

F

F

I

V

T

S

H
|
H

D

D

L

L

A

N

V

E

V

A

E

L

H

R

F

I

G

G

T

D

R

R

V

I

A

A

V

I

M

M

Y

K

L

D

G

G

R

K

V

I

C

M

E

Q

L

I

A

G

D

T

T

G

K

E

T

E

L

I

F

L

S

T

A

N

P

P

R

A

H

N

P

D

Y

Y

T

V

Q

K

binding adenosine-5'-triphosphate: T39 (≠ V39), S61 (= S61), G62 (= G62), S63 (≠ C63), G64 (= G64), K65 (= K65), S66 (= S66), T67 (= T67), Q111 (= Q110), K161 (≠ H165), Q162 (≠ E166), S164 (= S168), G166 (= G170), M167 (≠ Q171), Q188 (≠ E192), H221 (= H225)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 12, 29

4ymuJ Crystal structure of an amino acid abc transporter complex with arginines and atps (see paper)
33% identity, 69% coverage: 38:270/336 of query aligns to 13:239/240 of 4ymuJ

query
sites
4ymuJ

A

S

V
x
L

H

E

A
x
V

I

L

N

K

G

G

V

V

D

T

L

L

E

K

V

V

Q

N

K

K

G

G

E

E

A

V

L

V

C

V

V

I

G

G

E

P

S
|
S

G
|
G

C
x
S

G
|
G

K
|
K

S
|
S

T
|
T

V

L

A

L

R

R

T

C

V

I

M

N

G

L

L

L

L
x
E

S

E

P

P

S

T

A

K

G

G

E

E

I

V

H

F

Y

I

D

D

G

G

Q

V

R

K

I

I

D

N

N

N

L

-

E

G

R

K

K

V

D

N

S

I

L

N

P

K

Y

V

R

R

R

Q

K

K

M

V

Q

G

M

M

I

V

F

F

Q

Q

N

-

P

-

Y

H

A

F

S

N

L

L

N

F

P

P

R

H

M

L

T

T

-

A

I

I

Q

E

Q

N

T

I

L

T

E

L

E

A

P

P

I

V

R

K

F

V

H

K

P

M

D

N

W

K

S

K

P

-

V

-

Q

E

V

A

R

E

D

E

K

L

I

A

H

V

E

D

V

L

M

L

H

A

S

K

V

V

G

G

I

L

D

-

Q

L

D

D

W

K

G

K

N

D

R

Q

F

Y

G

P

H

I

E

K

F

L

S

S

G

G

Q

Q

R

K

Q

Q

R

R

I

L

A

A

I

I

A

A

R

R

A

A

L

L

A

A

V

M

D

Q

P

P

E

E

F

V

I

M

V

L

A

F

D

D

E
|
E

P

P

I

T

S

S

A

A

L

L

D

D

V

P

S

E

I

M

Q

V

A

K

Q

E

V

V

L

L

N

N

L

-

L

V

M

M

D

K

A

Q

Q

L

E

A

S

N

R

E

G

G

L

M

T

T

Y

M

L

V

F

V

I

V

T

T

H
|
H

D

E

L

M

A

G

V

F

V

A

E

R

H

E

F

V

G

G

T

D

R

R

V

V

A

I

V

F

M

M

Y

D

L

D

G

G

R

V

V

I

C

V

E

E

L

E

A

G

D

T

T

P

K

E

T

E

L

I

F

F

S

Y

A

R

P

A

R

K

H

N

P

E

Y

R

T

T

Q

R

A

E

L

F

L

L

S

S

A

K

I

I

binding adenosine-5'-triphosphate: L14 (≠ V39), V16 (≠ A41), S36 (= S61), G37 (= G62), S38 (≠ C63), G39 (= G64), K40 (= K65), S41 (= S66), T42 (= T67), E51 (≠ L76), E162 (= E192), H194 (= H225)
binding magnesium ion: S41 (= S66), E162 (= E192)

Sites not aligning to the query:

binding adenosine-5'-triphosphate: 11

2d62A Crystal structure of multiple sugar binding transport atp- binding protein
32% identity, 69% coverage: 39:271/336 of query aligns to 19:247/375 of 2d62A

query
sites
2d62A

V

V

H

T

A

A

I

V

N

K

G

D

V

L

D

S

L

L

E

E

V

I

Q

K

K

D

G

G

E

E

A

F

L

L

C

V

V

L

G

G

E
x
P

S
|
S

G
|
G

C
|
C

G
|
G

K
|
K

S
x
T

T
|
T

V

T

A

L

R

R

T

M

V

I

M

A

G

G

L

L

L

E

S

E

P

P

S

T

A

R

G

G

E

Q