SitesBLAST
Comparing GFF2088 FitnessBrowser__Phaeo:GFF2088 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q9CPU0 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Mus musculus (Mouse) (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 34:175/184 of Q9CPU0
- Q34 (≠ H7) binding
- E100 (= E56) binding
- H127 (= H76) binding in other chain
- E173 (= E119) binding in other chain
2za0A Crystal structure of mouse glyoxalase i complexed with methyl-gerfelin (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 31:172/180 of 2za0A
- active site: Q31 (≠ H7), E97 (= E56), H124 (= H76), E170 (= E119)
- binding methyl 4-(2,3-dihydroxy-5-methylphenoxy)-2-hydroxy-6-methylbenzoate: Q31 (≠ H7), F65 (= F40), L67 (= L42), F90 (vs. gap), E97 (= E56), H124 (= H76), M155 (= M107), E170 (= E119)
- binding zinc ion: Q31 (≠ H7), E97 (= E56), H124 (= H76), E170 (= E119)
4kyhA Crystal structure of mouse glyoxalase i complexed with zopolrestat (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 29:170/177 of 4kyhA
- active site: Q29 (≠ H7), E95 (= E56), H122 (= H76), E168 (= E119)
- binding zinc ion: Q29 (≠ H7), E95 (= E56), H122 (= H76), E168 (= E119)
- binding 3,4-dihydro-4-oxo-3-((5-trifluoromethyl-2-benzothiazolyl)methyl)-1-phthalazine acetic acid: M31 (= M9), R33 (= R11), F63 (= F40), E95 (= E56), T97 (= T58), N99 (= N60)
4kykA Crystal structure of mouse glyoxalase i complexed with indomethacin (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 29:170/179 of 4kykA
6l0uB Crystal structure of mouse glyoxalase i complexed with a small molecule inhibitor
35% identity, 81% coverage: 7:121/142 of query aligns to 27:168/177 of 6l0uB
- active site: Q27 (≠ H7), E93 (= E56), H120 (= H76), E166 (= E119)
- binding N-[4-(trifluoromethyloxy)phenyl]-1,3,4,9-tetrahydropyrido[3,4-b]indole-2-carbothioamide: R116 (= R72), K144 (≠ R100), D148 (= D104), G149 (= G105), W164 (≠ S117)
- binding zinc ion: Q27 (≠ H7), E93 (= E56), H120 (= H76), E166 (= E119)
4kykB Crystal structure of mouse glyoxalase i complexed with indomethacin (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 27:168/177 of 4kykB
- active site: Q27 (≠ H7), E93 (= E56), H120 (= H76), E166 (= E119)
- binding indomethacin: K144 (≠ R100), K150 (≠ H106), M151 (= M107), L154 (vs. gap), F156 (= F109)
- binding zinc ion: Q27 (≠ H7), E93 (= E56), H120 (= H76), E166 (= E119)
4pv5A Crystal structure of mouse glyoxalase i in complexed with 18-beta- glycyrrhetinic acid (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 22:163/170 of 4pv5A
4x2aA Crystal structure of mouse glyoxalase i complexed with baicalein (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 20:161/167 of 4x2aA
- active site: Q20 (≠ H7), E86 (= E56), H113 (= H76), E159 (= E119)
- binding 5,6,7-trihydroxy-2-phenyl-4H-chromen-4-one: Q20 (≠ H7), F49 (≠ S35), L56 (= L42), F79 (vs. gap), E86 (= E56), H113 (= H76), M144 (= M107), E159 (= E119)
- binding zinc ion: Q20 (≠ H7), E86 (= E56), H113 (= H76), E159 (= E119)
Sites not aligning to the query:
4opnA Crystal structure of mouse glyoxalase i complexed with mah
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/172 of 4opnA
- active site: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
- binding L-gamma-glutamyl-N-(3-ethynylphenyl)-N-hydroxy-L-glutaminylglycine: Q26 (≠ H7), R30 (= R11), F60 (= F40), F85 (vs. gap), E92 (= E56), T94 (= T58), N96 (= N60), R115 (= R72), H119 (= H76), K143 (≠ R100), K149 (≠ H106), M150 (= M107), L153 (vs. gap), E165 (= E119)
- binding zinc ion: Q26 (≠ H7), E92 (= E56)
Sites not aligning to the query:
7wt0A Human glyoxalase i (with c-ter his tag) in complex with tlsc702 (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 33:174/185 of 7wt0A
- binding (~{E})-3-(1,3-benzothiazol-2-yl)-4-(4-methoxyphenyl)but-3-enoic acid: C60 (≠ Y33), F62 (≠ S35), L69 (= L42), F71 (= F44), L92 (vs. gap), E99 (= E56), H126 (= H76), K150 (≠ R100), M157 (= M107), L160 (vs. gap), F162 (= F109), E172 (= E119)
- binding zinc ion: Q33 (≠ H7), E99 (= E56), H126 (= H76), E172 (= E119)
Sites not aligning to the query:
Q04760 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Homo sapiens (Human) (see 12 papers)
35% identity, 81% coverage: 7:121/142 of query aligns to 34:175/184 of Q04760
- Q34 (≠ H7) binding ; mutation to E: Reduces enzyme activity by 99%.
- S45 (= S18) mutation to A: No effect on phosphorylation.
- C61 (≠ Y33) modified: Disulfide link with 139, Alternate; mutation to A: No effect on NO-mediated modification.
- S69 (= S41) mutation to A: No effect on phosphorylation.
- S94 (vs. gap) mutation to A: No effect on phosphorylation.
- T98 (vs. gap) mutation to A: No effect on phosphorylation.
- E100 (= E56) binding ; mutation to Q: Reduces enzyme activity by over 99%.
- T102 (= T58) mutation to A: No effect on phosphorylation.
- T107 (≠ G63) modified: Phosphothreonine; mutation to A: Loss of phosphorylation.
- E111 (≠ L67) to A: in dbSNP:rs4746
- H127 (= H76) binding in other chain
- C139 (= C88) modified: S-glutathionyl cysteine; alternate; modified: Disulfide link with 61, Alternate; mutation to A: Impaired NO-mediated modification. Loss of NO-mediated modification; when associated with A-19 or A-20.
- E173 (= E119) active site, Proton donor/acceptor; binding in other chain; mutation to Q: Abolishes enzyme activity.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 2 modified: N-acetylalanine
- 19 modified: Disulfide link with 20; C → Y: in dbSNP:rs17855424; C→A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-20. Loss of NO-mediated modification; when associated with A-139.
- 20 modified: Disulfide link with 19; C→A: No effect on NO-mediated modification. Impaired NO-mediated modification; when associated with A-19. Loss of NO-mediated modification; when associated with A-139.
3w0uA Human glyoxalase i with an n-hydroxypyridone inhibitor
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/175 of 3w0uA
- active site: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
- binding N-[3-(1-Hydroxy-6-oxo-4-phenyl-1,6-dihydro-pyridin-2-yl)-5-methanesulfonylamino-phenyl]-methanesulfonamide: Q26 (≠ H7), F60 (= F40), L62 (= L42), E92 (= E56), H119 (= H76), K143 (≠ R100), M150 (= M107), E165 (= E119)
- binding zinc ion: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
Sites not aligning to the query:
3w0tA Human glyoxalase i with an n-hydroxypyridone derivative inhibitor
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/176 of 3w0tA
- active site: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
- binding N-[3-(1-hydroxy-6-oxo-4-phenyl-1,6-dihydropyridin-2-yl)phenyl]methanesulfonamide: Q26 (≠ H7), F55 (≠ S35), F60 (= F40), L62 (= L42), F64 (= F44), E92 (= E56), H119 (= H76), E165 (= E119)
- binding zinc ion: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
Sites not aligning to the query:
3vw9A Human glyoxalase i with an n-hydroxypyridone inhibitor (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/176 of 3vw9A
- active site: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
- binding 1-hydroxy-6-[1-(3-methoxypropyl)-1H-pyrrolo[2,3-b]pyridin-5-yl]-4-phenylpyridin-2(1H)-one: Q26 (≠ H7), C53 (≠ Y33), L62 (= L42), E92 (= E56), H119 (= H76), M150 (= M107), F155 (= F109), E165 (= E119)
- binding zinc ion: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
Sites not aligning to the query:
1qipA Human glyoxalase i complexed with s-p- nitrobenzyloxycarbonylglutathione (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/176 of 1qipA
- active site: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
- binding s-p-nitrobenzyloxycarbonylglutathione: R30 (= R11), Q51 (≠ K31), C53 (≠ Y33), F60 (= F40), F64 (= F44), I81 (vs. gap), L85 (vs. gap), T94 (= T58), N96 (= N60), R115 (= R72), M150 (= M107), F155 (= F109)
- binding zinc ion: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
Sites not aligning to the query:
1qinA Human glyoxalase i complexed with s-(n-hydroxy-n-p- iodophenylcarbamoyl) glutathione (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/176 of 1qinA
- active site: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
- binding s-(n-hydroxy-n-iodophenylcarbamoyl)glutathione: Q26 (≠ H7), R30 (= R11), F60 (= F40), L62 (= L42), F64 (= F44), E92 (= E56), T94 (= T58), N96 (= N60), R115 (= R72), H119 (= H76), K143 (≠ R100), G148 (= G105), K149 (≠ H106), M150 (= M107), E165 (= E119)
- binding zinc ion: Q26 (≠ H7), E92 (= E56)
Sites not aligning to the query:
1froA Human glyoxalase i with benzyl-glutathione inhibitor (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/176 of 1froA
- active site: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
- binding s-benzyl-glutathione: R30 (= R11), F60 (= F40), T94 (= T58), N96 (= N60), R115 (= R72), M150 (= M107), F155 (= F109), E165 (= E119)
- binding zinc ion: Q26 (≠ H7), E92 (= E56), H119 (= H76), E165 (= E119)
Sites not aligning to the query:
7wszA Human glyoxalase i (with c-ter his tag) in glycerol-bound form (see paper)
35% identity, 81% coverage: 7:121/142 of query aligns to 26:167/183 of 7wszA
Sites not aligning to the query:
2c21A Specificity of the trypanothione-dependednt leishmania major glyoxalase i: structure and biochemical comparison with the human enzyme (see paper)
35% identity, 97% coverage: 4:141/142 of query aligns to 3:138/139 of 2c21A
P0AC81 Lactoylglutathione lyase; Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase; EC 4.4.1.5 from Escherichia coli (strain K12) (see paper)
36% identity, 87% coverage: 3:126/142 of query aligns to 1:129/135 of P0AC81
- H5 (= H7) binding
- E56 (= E56) binding
- H74 (= H76) binding in other chain
- E122 (= E119) binding in other chain
Query Sequence
>GFF2088 FitnessBrowser__Phaeo:GFF2088
MPLKYLHTMVRVKDLEKSMAFYALLGLKETKRYDSEKGRFSLIFMAAEGQEDTPIELTYN
WDGDDALPSDSRHFGHLAYGVDDIYATCQHLMDNGVTINRPPRDGHMAFVRSPDNISIEL
LQNGDALDPAEPWASMENTGSW
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory