SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2091 FitnessBrowser__psRCH2:GFF2091 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

Q6LX42 Sodium/alanine symporter AgcS; Alanine permease; Alanine/glycine:cation symporter from Methanococcus maripaludis (strain S2 / LL) (see paper)
52% identity, 99% coverage: 1:443/447 of query aligns to 1:442/453 of Q6LX42

query
sites
Q6LX42

M

M

E

D

F

F

L

V

N

S

N

-

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L

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V

N

N

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T

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N

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Y

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T75 (= T77) binding ; binding
G79 (= G81) binding
N80 (= N82) binding ; mutation to A: Markedly reduces L-alanine uptake; when associated with A-274 and A-308.
I165 (= I165) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
Q170 (= Q170) binding ; binding
F273 (= F277) mutation to A: Shows poor substrate selectivity, allowing additional amino acids such as L-valine and L-leucine to be efficiently transported. Decreases L-alanine uptake.
FS 273:274 (= FS 277:278) binding
FSNE 273:276 (= FSNE 277:280) binding
S274 (= S278) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-308.
D308 (= D312) mutation to A: Markedly reduces L-alanine uptake; when associated with A-80 and A-274.
W370 (= W371) mutation to Q: Does not change uptake of L-alanine, but reduces the ability to transport D-alanine.

6csfM Crystal structure of sodium/alanine symporter agcs with d-alanine bound (see paper)
53% identity, 96% coverage: 16:443/447 of query aligns to 1:422/431 of 6csfM

query
sites
6csfM

W

W

G

G

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Y

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x
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G

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A

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G

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D

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T

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F

binding d-alanine: T58 (= T77), G60 (= G79), G62 (= G81), N63 (= N82), Q150 (= Q170), F253 (= F277), S254 (= S278)
binding sodium ion: T58 (= T77), I59 (≠ V78), N63 (= N82), S254 (= S278), D288 (= D312)

6cseM Crystal structure of sodium/alanine symporter agcs with l-alanine bound (see paper)
53% identity, 96% coverage: 16:443/447 of query aligns to 1:422/431 of 6cseM

query
sites
6cseM

W

W

G

G

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Y

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M

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A

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G

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W

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G

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F

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S

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E

A

A

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L

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A

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G

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-

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G

A

A

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A

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T

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A

G

L

L

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F

Y

T

S

T

T

I

V

L

L

G

G

W

W

S

S

Y

Y

F

Y

G

G

E

E

K

K

C

C

W

F

E

E

F

Y

L

L

V

I

G

G

T

T

R

K

A

G

I

I

W

R

P

L

F

Y

R

R

V

I

I

A

W

F

V

V

L

L

A

V

V

A

P

F

F

W

G

G

A

A

I

T

A

A

Q

S

L

L

D

P

F

L

A

V

W

W

L

N

L

I

A

A

D

D

T

T

L

L

N

N

G

G

L

A

M

M

A

A

I

I

P

P

N

N

L

L

I

I

S

G

L

L

L

L

L

L

L

L

S

S

P

G

V

V

V

V

V

V

K

S

L

E

T

T

K

K

E

A

Y

F

binding alanine: A57 (= A76), T58 (= T77), G60 (= G79), N63 (= N82), Q150 (= Q170), F253 (= F277), S254 (= S278), E256 (= E280)
binding sodium ion: T58 (= T77), I59 (≠ V78), N63 (= N82), S254 (= S278), D288 (= D312)

Query Sequence

>GFF2091 FitnessBrowser__psRCH2:GFF2091
MEFLNNLVNSVNGLVWGPPMLVLILGTGLFLMIFLKFMPLTRIPTGFALMWRGRTKGDEA
TGEISPFQALMTSLAATVGTGNIAGVATAIFLGGPGALFWMWCTALVGMATKYCEVVLAV
HYREKDDRGEHVGGPMYAIKNGLGKKWIWLGTAFAIFGGLAGFGIGNMVQVNSMAHALET
TFAVPLWATGLVTMVIVGLVILGGIRRIGVVAASLVPFMCLAYLIAAAVVLVVNVSAIPA
AFDLIFTHAFTPIAATGGFAGAAVMAAIRFGVARGIFSNEAGLGTAGIAQAAGTTTSSVR
SGMIGMLGTFIDTIIVCSMTGLAIICTGVWTSGESGAALSAAAFESAMPGIGGIILTIAL
VVFAFTTILGWSYFGEKCWEFLVGTRAIWPFRVIWVLAVPFGAIAQLDFAWLLADTLNGL
MAIPNLISLLLLSPVVVKLTKEYFARS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory