SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2122 FitnessBrowser__Phaeo:GFF2122 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5LUF3 Propionyl-CoA carboxylase alpha chain; EC 6.4.1.3 from Ruegeria pomeroyi (strain ATCC 700808 / DSM 15171 / DSS-3) (Silicibacter pomeroyi) (see paper)
88% identity, 100% coverage: 1:681/681 of query aligns to 1:681/681 of Q5LUF3

query
sites
Q5LUF3

M

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F

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E

F348 (= F348) binding biotin
W515 (= W515) mutation to L: No effect on holoenzyme formation.
L599 (= L599) mutation to A: Loss of holoenzyme formation; when associated with A-602 and A-603.
L602 (= L602) mutation to A: Loss of holoenzyme formation; when associated with A-602 and A-603.
M603 (= M603) mutation to A: No effect on holoenzyme formation. Loss of holoenzyme formation; when associated with A-602 and A-603.
K647 (= K647) modified: N6-biotinyllysine

3n6rG Crystal structure of the holoenzyme of propionyl-coa carboxylase (pcc) (see paper)
84% identity, 100% coverage: 2:681/681 of query aligns to 1:646/646 of 3n6rG

query
sites
3n6rG

F

F

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L

L

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A

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D

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K

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A

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F

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L

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L

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P

P

E

E

K

K

I

L

A

P

P

P

D

D

T

T

S

S

K

K

L

M

L

L

L

L

C

C

P

P

M

M

P

P

G

G

L

L

V

I

V

V

K

K

L

V

E

D

V

V

E

E

V

V

G

G

D

Q

E

E

V

V

Q

Q

E

E

G

G

Q

Q

A

A

L

L

C

C

T

T

I

I

E

E

A

A

M
|
M

K
|
K

M

M

E

E

N

N

I

I

L

L

R

R

A

A

E

E

K

K

K

K

G

G

V

V

V

V

S

A

A

K

I

I

N

N

A

A

A

S

A

A

G

G

D

N

S

S

L

L

A

A

V

V

D

D

E

D

V

V

I

I

M

M

E

E

F

F

E

E

active site: K115 (= K116), K157 (= K158), D180 (= D195), H193 (= H208), R219 (= R234), T258 (= T273), E260 (= E275), E273 (= E288), N275 (= N290), R277 (= R292), E281 (= E296), R323 (= R338), G519 (= G554)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M611 (= M646), K612 (= K647)

3n6rA Crystal structure of the holoenzyme of propionyl-coa carboxylase (pcc) (see paper)
83% identity, 70% coverage: 203:681/681 of query aligns to 133:591/591 of 3n6rA

query
sites
3n6rA

V

V

T

T

Q

Q

P

P

R

R

H
|
H

I

I

E

E

I

I

Q

Q

V

V

L

L

C

C

D

D

A

S

H

H

G

G

N

N

G

G

I

I

Y

Y

L

L

G

G

E

E

R

R

E

E

C

C

S

S

I

I

Q

Q

R

R

R
|
R

N

N

Q

Q

K

K

V

V

V

V

E

E

E

E

A

A

P

P

S

S

P

P

F

F

L

L

D

D

E

E

D

A

T

T

R

R

R

R

A

A

M

M

G

G

E

E

Q

Q

A

A

V

V

A

A

L

L

A

A

K

K

A

A

V

V

G

G

Y

Y

A

A

S

S

A

A

G

G

T
|
T

V

V

E
|
E

F

F

I

I

V

V

D

D

G

G

D

Q

K

K

N

N

F

F

Y

Y

F

F

L

L

E
|
E

M

M

N
|
N

T

T

R
|
R

L

L

Q

Q

V

V

E
|
E

H

H

P

P

V

V

T

T

E

E

L

L

I

I

T

T

G

G

V

V

D

D

L

L

V

V

E

E

Q

Q

M

M

I

I

R

R

I

V

A

A

G

A

G

G

E

E

P

P

L

L

S

S

I

I

T

T

Q

Q

N

G

D

D

V

V

K

K

L

L

N

T

G

G

W

W

A

A

I

I

E

E

N

N

R
|
R

L

L

Y

Y

A

A

E

E

D

D

P

P

Y

Y

R

R

G

G

F

F

L

L

P

P

S

S

I

I

G

G

R

R

L

L

T

T

R

R

Y

Y

R

R

P

P

P

P

A

A

E

E

V

A

A

A

A

-

G

-

P

-

L

-

H

-

D

-

S

-

G

-

K

-

W

-

Q

-

G

-

D

-

A

-

P

-

A

-

G

-

E

-

L

-

A

-

V

V

R

R

N

N

D

D

T

T

G

G

V

V

F

Y

E

E

G

G

G

G

E

E

I

I

S

S

M

M

Y

Y

Y

Y

D

D

P

P

M

M

I

I

A

A

K

K

L

L

C

C

T

T

W

W

G

A

P

P

T

T

R

R

D

A

A

A

A

A

I

I

A

E

A

A

M

M

R

R

D

I

A

A

L

L

D

D

G

S

F

F

E

E

V

V

E

E

G

G

I

I

G

G

H

H

N

N

L

L

P

P

F

F

L

L

S

S

A

A

V

V

M

M

D

D

H

H

P

P

I

K

F

F

I

I

A

S

G

G

T

D

M

M

T

T

T

T

A

A

F

F

I

I

E

A

E

E

Q

E

Y

Y

P

P

E

E

G

G

F

F

N

E

G

G

V

V

E

N

L

L

P

P

E

E

Q

T

D

D

L

L

R

R

K

R

I

V

A

A

A

A

A

A

S

A

A

A

A

A

M

M

H

H

R

R

V

V

A

A

E

E

I

I

R

R

R

R

T

T

R

R

V

V

S

S

G

G

R

R

M

M

D

D

N

N

H

H

E

E

R

R

K

R

V

V

G

G

S

T

D

E

W

W

V

V

V

V

T

T

L

L

Q

Q

D

G

V

A

S

D

F

F

N

P

V

V

T

T

V

I

D

A

A

A

D

D

R

H

D

D

G

G

S

S

T

T

V

V

R

S

F

F

D

D

D

D

G

G

S

S

A

S

L

M

R

R

V

V

A

T

S

S

D

D

W

W

T

T

P

P

G
|
G

D

D

Q

Q

L

L

A

A

R

N

L

L

T

M

V

V

G

D

D

G

D

A

S

P

L

L

V

V

V

L

K

K

V

V

G

G

K

K

I

I

S

S

G

G

G

G

F

F

R

R

I

I

R

R

S

T

R

R

G

G

A

A

D

D

L

L

K

K

V

V

H

H

V

V

R

R

T

T

P

P

R

R

Q

Q

A

A

E

E

L

L

A

A

K

R

L

L

M

M

P

P

E

E

K

K

I

L

A

P

P

P

D

D

T

T

S

S

K

K

L

M

L

L

L

L

C

C

P

P

M

M

P

P

G

G

L

L

V

I

V

V

K

K

L

V

E

D

V

V

E

E

V

V

G

G

D

Q

E

E

V

V

Q

Q

E

E

G

G

Q

Q

A

A

L

L

C

C

T

T

I

I

E

E

A

A

M
|
M

K
|
K

M

M

E

E

N

N

I

I

L

L

R

R

A

A

E

E

K

K

K

K

G

G

V

V

V

V

S

A

A

K

I

I

N

N

A

A

A

S

A

A

G

G

D

N

S

S

L

L

A

A

V

V

D

D

E

D

V

V

I

I

M

M

E

E

F

F

E

E

active site: H138 (= H208), R164 (= R234), T203 (= T273), E205 (= E275), E218 (= E288), N220 (= N290), R222 (= R292), E226 (= E296), R268 (= R338), G464 (= G554)
binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M556 (= M646), K557 (= K647)

Sites not aligning to the query:

active site: 115

7ybuA Human propionyl-coenzyme a carboxylase
53% identity, 100% coverage: 2:681/681 of query aligns to 5:670/670 of 7ybuA

query
sites
7ybuA

F

F

E

D

K

K

I

I

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

C

C

R

R

V

V

I

I

K

R

T

T

A

C

R

K

K

K

M

M

G

G

I

I

K

K

T

T

V

V

A

A

I

I

Y

H

S

S

D

D

A

V

D

D

K

A

Q

S

S

S

L

V

H

H

V

V

K

K

M

M

A

A

D

D

E

E

A

A

V

V

H

C

I

V

G

G

P

P

P

A

P

P

A

T

N

S

Q

K

S

S

Y

Y

I

L

V

N

I

M

D

D

K

A

V

I

M

M

E

E

A

A

I

I

R

K

A

K

T

T

G

R

A

A

Q

Q

A

A

V

V

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

K

K

E

F

F

A

A

E

R

A

C

L

L

A

A

A

A

E

E

G

D

V

V

A

V

F

F

V

I

G

G

P

P

P

D

V

T

G

H

A

A

I

I

E

Q

S

A

M

M

G

G

D

D

K

K

I

I

T

E

S

S

K

K

K

L

I

L

A

A

Q

K

E

K

A

A

G

E

V

V

S

N

T

T

V

I

P

P

G

G

Y

F

M

D

G

G

L

V

I

V

A

K

D

D

A

A

D

E

E

E

A

A

V

V

K

R

I

I

S

A

N

R

E

E

I

I

G

G

Y

Y

P

P

V

V

M

M

I

I

K

K

A

A

S

S

A

A

G

G

G

G

G

G

G

G

K

K

G

G

M

M

R

R

I

I

A

A

W

W

S

D

D

D

A

E

E

E

A

T

R

R

E

D

G

G

F

F

Q

R

S

L

S

S

K

S

N

Q

E

E

A

A

A

A

N

S

S

S

F

F

G

G

D

D

D

D

R

R

I

L

F

L

I

I

E

E

K

K

F

F

V

I

T

D

Q

N

P

P

R

R

H

H

I

I

E

E

I

I

Q

Q

V

V

L

L

C

G

D

D

A

K

H

H

G

G

N

N

G

A

I

L

Y

W

L

L

G

N

E

E

R

R

E

E

C

C

S

S

I

I

Q

Q

R

R

R

R

N

N

Q

Q

K

K

V

V

V

V

E

E

E

E

A

A

P

P

S

S

P

I

F

F

L

L

D

D

E

A

D

E

T

T

R

R

R

R

A

A

M

M

G

G

E

E

Q

Q

A

A

V

V

A

A

L

L

A

A

K

R

A

A

V

V

G

K

Y

Y

A

S

S

S

A

A

G

G

T

T

V

V

E

E

F

F

I

L

V

V

D

D

G

S

D

K

K

K

N

N

F

F

Y

Y

F

F

L

L

E

E

M

M

N

N

T

T

R

R

L

L

Q

Q

V

V

E

E

H

H

P

P

V

V

T

T

E

E

L

C

I

I

T

T

G

G

V

L

D

D

L

L

V

V

E

Q

Q

E

M

M

I

I

R

R

I

V

A

A

G

K

G

G

E

Y

P

P

L

L

S

R

I

H

T

K

Q

Q

N

A

D

D

V

I

K

R

L

I

N

N

G

G

W

W

A

A

I

V

E

E

N

C

R

R

L

V

Y

Y

A

A

E

E

D

D

P

P

Y

Y

R

K

G

S

F

F

-

G

L

L

P

P

S

S

I

I

G

G

R

R

L

L

T

S

R

Q

Y

Y

R

Q

P

E

P

-

A

-

E

-

V

-

A

-

A

-

G

-

P

P

L

L

H

H

D

L

S

P

G

G

K

-

W

-

Q

-

G

-

D

-

A

-

P

-

A

-

G

-

E

-

L

-

A

-

V

V

R

R

N

V

D

D

T

S

G

G

V

I

F

Q

E

P

G

G

G

S

E

D

I

I

S

S

M

I

Y

Y

Y

Y

D

D

P

P

M

M

I

I

A

S

K

K

L

L

C

I

T

T

W

Y

G

G

P

S

T

D

R

R

D

T

A

E

A

A

I

L

A

K

A

R

M

M

R

A

D

D

A

A

L

L

D

D

G

N

F

Y

E

V

V

I

E

R

G

G

I

V

G

T

H

H

N

N

L

I

P

A

F

L

L

L

S

R

A

E

V

V

M

I

D

I

H

N

P

S

I

R

F

F

I

V

A

K

G

G

T

D

M

I

T

S

T

T

A

K

F

F

I

L

E

S

E

D

Q

V

Y

Y

P

P

E

D

G

G

F

F

N

K

G

G

V

H

E

M

L

L

P

T

E

K

Q

S

D

E

L

K

R

N

K

Q

I

L

A

L

A

A

A

I

S

A

A

S

A

S

M

L

H

F

R

V

V

A

A

F

E

Q

I

L

R

R

R

A

T

Q

R

H

V

F

-

Q

-

E

S

N

G

S

R

R

M

M

D

P

N

V

H

I

E

K

R

P

K

D

V

I

G

-

S

A

D

N

W

W

V

E

V

L

T

S

L

V

Q

K

D

L

V

H

S

D

F

K

N

V

V

H

T

T

V

V

D

V

A

A

D

S

R

N

D

N

G

G

S

S

T

V

V

F

R

S

F

V

D

E

-

V

D

D

G

G

S

S

A

K

L

L

R

N

V

V

A

T

S

S

D

T

W

W

T

N

P

L

G

A

D

S

Q

P

L

L

A

L

R

S

L

V

T

S

V

V

-

D

G

G

D

T

D

Q

S

R

L

T

V

V

V

Q

K

C

V

L

G

S

K

R

I

E

S

A

G

G

G

G

-

N

F

M

R

S

I

I

R

Q

S

F

R

L

G

G

A

T

D

V

L

Y

K

K

V

V

H

N

V

I

R

L

T

T

P

R

R

L

Q

A

A

A

E

E

L

L

A

N

K

K

L

F

M

M

P

L

E

E

K

K

I

V

A

T

P

E

D

D

T

T

S

S

K

S

L

V

L

L

L

R

C

S

P

P

M

M

P

P

G

G

L

V

V

V

V

V

K

A

L

V

E

S

V

V

E

K

V

P

G

G

D

D

E

A

V

V

Q

A

E

E

G

G

Q

Q

A

E

L

I

C

C

T

V

I

I

E

E

A

A

M
|
M

K
|
K

M

M

E

Q

N

N

I

S

L

M

R

T

A

A

E

G

K

K

K

T

G

G

V

T

V

V

S

K

A

S

I

V

N

H

A

C

A

Q

A

A

G

G

D

D

S

T

L

V

A

G

V

E

D

G

E

D

V

L

I

L

M

V

E

E

F

L

E

E

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M635 (= M646), K636 (= K647)

P05165 Propionyl-CoA carboxylase alpha chain, mitochondrial; PCCase subunit alpha; Propanoyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.3 from Homo sapiens (Human) (see 6 papers)
53% identity, 100% coverage: 2:681/681 of query aligns to 63:728/728 of P05165

query
sites
P05165

F

F

E

D

K

K

I

I

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A
|
A

C

C

R
|
R

V

V

I

I

K

R

T

T

A

C

R

K

K

K

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A75 (= A14) to P: in PA-1; dbSNP:rs794727479
R77 (= R16) to W: in PA-1; loss of function; dbSNP:rs141371306
A138 (= A77) to T: in PA-1; loss of function; dbSNP:rs202247814
I164 (≠ V103) to T: in PA-1; loss of function; dbSNP:rs202247815
G197 (= G136) to E: in PA-1
M229 (= M168) to K: in PA-1; dbSNP:rs375628794
Q297 (= Q236) to R: in PA-1
D368 (= D307) to G: in PA-1
M373 (= M312) to K: in PA-1; unstable protein; loss of function; dbSNP:rs121964958
G379 (= G318) to V: in PA-1; dbSNP:rs794727087
C398 (≠ N337) to R: in PA-1
R399 (= R338) to Q: in PA-1; dbSNP:rs1301904623
P423 (= P368) to L: in PA-1; dbSNP:rs1443858896
L532 (≠ M489) natural variant: Missing (in PA-1)
V551 (≠ N506) to F: in dbSNP:rs61749895
W559 (= W515) to L: in PA-1; dbSNP:rs118169528
G631 (= G584) to R: in PA-1; loss of function; dbSNP:rs796052018
G668 (= G621) to R: in PA-1; loss of biotinylation; dbSNP:rs771438170
K694 (= K647) modified: N6-biotinyllysine; by HLCS
C712 (≠ A665) natural variant: Missing (in PA-1; loss of biotinylation)

Sites not aligning to the query:

1:52 modified: transit peptide, Mitochondrion

8sgxX Leishmania tarentolae propionyl-coa carboxylase (alpha-4-beta-6) (see paper)
46% identity, 99% coverage: 5:681/681 of query aligns to 1:657/657 of 8sgxX

query
sites
8sgxX

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M

R

R

G

G

A

T

D

T

L

F

K

D

V

V

H

N

V

V

R

M

T

S

P

D

R

L

Q

Q

A

S

E

T

L

L

A

A

K

H

L

F

M

V

P

P

E

I

K

T

I

E

A

A

P

T

D

T

T

N

S

T

K

K

L

Q

L

I

L

L

C

S

P

P

M

M

P

P

G

G

L

V

V

I

V

V

K

A

L

I

E

K

V

V

E

Q

V

P

G

G

D

Q

E

M

V

V

Q

V

E

A

G

G

Q

E

A

E

L

L

C

L

T

T

I

L

E

E

A

A

M
|
M

K
|
K

M

M

E

R

N

N

I

K

L

I

R

H

A

A

E

Q

K

A

K

D

G

G

V

K

V

V

S

K

A

E

I

V

N

K

A

V

A

K

A

L

G

G

D

A

S

T

L

V

A

E

V

D

D

N

E

E

V

V

I

L

M

V

E

E

F

L

E

E

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M622 (= M646), K623 (= K647)

8rthA Trypanosoma brucei 3-methylcrotonyl-coa carboxylase (see paper)
42% identity, 98% coverage: 2:668/681 of query aligns to 2:651/666 of 8rthA

query
sites
8rthA

F

F

E

K

K

K

I

V

L

L

I

V

A

A

N

N

R

R

G

G

E

E

I

I

A

A

C

C

R

R

V

V

I

F

K

R

T

T

A

C

R

R

K

E

M

M

G

N

I

I

K

R

T

T

V

V

A

A

I

V

Y

C

S

C

D

E

A

G

D

E

K

P

Q

N

S

A

L

K

H

H

V

V

K

L

M

E

A

A

D

D

E

E

A

A

V

F

H

V

I

L

G

G

P

P

P

P

P

P

A

A

N

S

Q

T

S

S

Y

Y

I

L

V

R

I

G

D

D

K

R

V

I

M

I

E

C

A

A

I

A

R

K

A

K

T

L

G

Q

A

A

Q

D

A

A

V

V

H

H

P

P

G

G

Y

Y

G

G

F

F

L

L

S

S

E

E

N

N

A

A

K

E

F

F

A

A

E

S

A

A

L

V

A

L

A

A

E

A

G

G

V

L

A

K

F

F

V

V

G

G

P

P

P

P

V

P

G

A

A

A

I

M

E

L

S

S

M

M

G

G

D

S

K

K

I

S

T

E

S

S

K

K

K

R

I

I

A

M

Q

E

E

A

A

A

G

G

V

V

S

P

T

I

V

V

P

P

G

G

Y

Y

M

Y

G

G

L

E

I

D

A

Q

D

N

A

P

D

D

E

R

A

L

V

L

K

H

I

E

S

A

N

K

E

T

I

I

G

G

Y

F

P

P

V

V

M

L

I

I

K

K

A

A

S

V

A

S

G

G

G

G

G

G

G

G

K

K

G

G

M

M

R

K

I

I

A

V

W

M

S

E

D

E

A

T

E

E

A

F

R

H

E

L

G

M

F

L

Q

E

S

S

S

A

K

K

N

R

E

E

A

A

A

I

N

N

S

F

F

F

G

K

D

D

D

D

R

R

I

V

F

I

I

L

E

E

K

R

F

Y

V

V

T

M

Q

H

P

P

R

R

H

H

I

I

E

E

I

C

Q

Q

V

I

L

F

C

F

D

D

A

S

H

F

G

G

N

N

G

G

I

V

Y

F

L

F

G

F

E

E

R

R

E

D

C

C

S

S

I

V

Q

Q

R

R

R

R

N

H

Q

Q

K

K

V

V

V

I

E

E

E

E

A

A

P

P

S

A

P

P

F

G

L

L

D

S

E

V

D

D

T

M

R

R

R

R

A

R

M

I

G

G

E

D

Q

V

A

A

V

L

A

T

L

A

A

A

K

R

A

A

V

V

G

G

Y

Y

A

V

S

G

A

A

G

G

T

T

V

V

E

E

F

F

I

I

V

F

D

D

G

T

D

E

K

K

N

D

-

E

F

F

Y

F

F

F

L

M

E

E

M

M

N

N

T

T

R

R

L

L

Q

Q

V

V

E

E

H

H

P

P

V

V

T

T

E

E

L

Q

I

C

T

Q

-

V

-

R

-

G

-

R

G

P

V

L

D

D

L

L

V

V

E

R

Q

L

M

Q

I

L

R

Q

I

T

A

A

G

M

G

G

E

L

P

P

L

L

S

G

I

F

T

R

Q

Q

N

E

D

D

V

I

K

S

L

M

N

S

G

G

W

A

A

S

I

V

E

E

N

A

R

R

L

I

Y

Y

A

A

E

E

D

S

P

P

Y

R

R

N

G

G

F

F

L

L

P

P

S

V

I

G

G

G

R

R

L

L

T

R

R

Y

Y

L

R

K

P

E

P

P

A

P

E

-

V

-

A

-

A

-

G

-

P

-

L

-

H

-

D

-

S

-

G

-

K

-

W

-

Q

Q

G

G

D

N

A

-

P

-

A

R

G

G

E

T

L

V

A

K

V

V

R

R

N

L

D

D

T

T

G

G

V

F

F

R

E

A

G

G

G

D

E

D

I

V

S

L

M

V

Y

H

Y

Y

D

D

P

P

M

M

I

I

A

A

K

K

L

L

C

V

T

V

W

W

G

G

P

D

T

N

R

R

D

A

A

T

A

A

I

L

A

E

A

G

M

L

R

R

D

T

A

A

L

L

D

A

G

S

F

Y

E

H

V

I

E

V

G

G

I

V

G

E

H

T

N

N

L

I

P

D

F

F

L

L

S

Q

A

C

V

C

M

L

D

S

H

N

P

P

I

G

F

F

I

V

A

E

G

G

T

G

M

V

T

T

T

T

A

R

F

F

I

I

E

E

E

D

Q

N

Y

S

P

V

E

N

G

L

F

L

N

Q

G

P

V

R

E

E

L

I

P

P

E

N

Q

N

D

V

L

L

R

-

K

-

I

-

A

-

A

-

A

A

S

L

A

A

A

A

M

V

H

S

R

Y

V

L

A

C

E

S

I

Q

R

R

R

G

T

T

R

S

V

-

S

T

G

L

R

F

M

W

D

P

N

N

H

R

E

Q

R

I

K

S

V

Q

G

G

S

V

D

C

W

F

V

T

V

V

T

G

L

G

Q

N

D

P

V

V

S

V

F

V

N

R

V

V

T

T

V

V

D

-

A

S

D

T

R

K

D

M

G

C

S

F

T

T

V

C

R

D

F

F

D

D

D

S

G

S

S

S

A

V

L

T

R

V

V

Y

A

V

S

E

D

S

W

T

T

T

P

-

G

-

D

-

Q

-

L

-

A

-

R

-

L

-

T

N

V

M

G

P

D

D

D

S

S

S

L

T

V

F

V

I

K

R

V

V

-

T

-

V

-

D

G

G

K

E

I

T

S

R

G

F

G

G

F

F

R

-

I

-

R

T

S

S

R

F

G

V

A

T

D

D

L

S

K

E

V

V

H

A

V

V

R

A

T

L

P

P

R

Q

Q

G

A

F

E

Y

L

T

A

L

K

A

L

L

M

Q

P

P

-

L

-

A

-

T

-

D

-

F

-

G

-

S

E

T

K

S

I

A

A

Q

P

A

D

N

T

G

S

S

K

A

L

S

L

V

L

L

C

S

P

P

M

M

P

P

G

G

L

K

V

V

V

T

K

K

L

L

E

L

V

V

E

A

V

D

G

G

D

T

E

L

V

V

Q

Q

E

Q

G

G

Q

Q

A

A

L

I

C

L

T

I

I

L

E

E

A

A

M
|
M

K
|
K

M

M

E

E

N

H

I

V

L

V

R

K

A

A

E

S

K

C

K

D

G

G

V

E

V

V

S

K

A

F

I

C

N

V

A

H

A

A

A

D

G

G

binding 5-(hexahydro-2-oxo-1h-thieno[3,4-d]imidazol-6-yl)pentanal: M629 (= M646), K630 (= K647)

8j78I Human 3-methylcrotonyl-coa carboxylase in bccp-h2 state
39% identity, 100% coverage: 4:681/681 of query aligns to 6:649/651 of 8j78I

query
sites
8j78I

K

K

I

V

L

L

I

I

A

A

N

N

R

R

G

G

E

E

I

I

A

A

C

C

R

R

V

V

I

M

K

R

T

T

A

A

R

K

K

K

M

L

G

G

I

V

K

Q

T

T

V

V

A

A

I

V

Y

Y

S

S

D

E

A

A

D

D

K

R

Q

N

S

S

L

M

H

H

V

V

K

D

M

M

A

A

D

D

E

E

A

A

V

Y

H

S

I

I

G

G

P

P

P

A

P

P

A

S

N

Q

Q

Q

S

S

Y

Y

I

L

V

S

I

M

D

E

K

K

V

I

M

I

E

Q

A

V

I

A

R

K

A

T

T

S

G

A

A

A

Q

Q

A

A

V

I

H

H

P

P

G

G

Y

C

G

G

F

F

L

L

S

S

E

E

N

N

A

M

K

E

F

F

A

A

E

E

A

L

L

C

A

K

A

Q

E

E

G

G

V

I

A

I

F

F

V

I

G

G

P

P

P

P

V

P

G

S

A

A

I

I

E

R

S

D

M

M

G

G

D

I

K

K

I

S

T

T

S

S

K

K

K

S

I

I

A

M

Q

A

E

A

A

A

G

G

V

V

S

P

T

V

V

V

P

E

G

G

Y

Y

M

H

G

G

L

E

I

-

A

D

D

Q

A

S

D

D

E

Q

A

C

V

L

K

K