SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2124 PS417_10835 major facilitator transporter to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P53322 High-affinity nicotinic acid transporter; Nicotinic acid permease from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 64% coverage: 1:280/438 of query aligns to 73:353/534 of P53322

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P53322

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K283 (≠ A217) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Q9NRA2 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Membrane glycoprotein HP59; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Homo sapiens (Human) (see 8 papers)
24% identity, 65% coverage: 65:348/438 of query aligns to 113:402/495 of Q9NRA2

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Q9NRA2

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K136 (≠ R88) to E: in SD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transporter activity, but retains appreciable H(+)-coupled sialic acid transporter activity; dbSNP:rs80338795
H183 (≠ I133) to R: in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity; dbSNP:rs119491109
LL 198:199 (≠ AI 148:149) mutation to AA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
IL 266:267 (≠ LE 223:224) mutation to LA: Localizes in vesicular structures mainly concentrated in the perinuclear region.
SSLRN 268:272 (≠ TELGT 225:229) natural variant: Missing (in ISSD; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; abolishes H(+)-coupled sialic acid transporter activity; has normal L-aspartate and L-glutamate transporter activity)
G328 (= G283) to E: in ISSD; some patients may manifest a milder phenotype consistent with Salla disease; markedly decreases H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs386833996
P334 (= P289) to R: in ISSD; does not affect intracellular localization, targeted to lysosomes; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs119491110
G371 (≠ P317) to V: in ISSD; abolishes H(+)-coupled sialic acid transporter activity; abolishes L-aspartate and L-glutamate transporter activity; dbSNP:rs777862172

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane.; LL→GG: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R → C: in SD; frequent variant in Finland; alters intracellular localization, only partially targeted to lysosomes and mainly detected in LAMP1-negative vesicles and in the Golgi apparatus; completely devoid of L-aspartate and L-glutamate transport activity, but retains appreciable H(+)-coupled sialic acid and nitrate transporter activity; dbSNP:rs80338794

Q8BN82 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 member 5; Vesicular excitatory amino acid transporter; VEAT from Mus musculus (Mouse) (see paper)
22% identity, 65% coverage: 65:348/438 of query aligns to 113:402/495 of Q8BN82

query
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Q8BN82

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H183 (≠ I133) mutation to R: Abolishes sialic acid transporter activity. Does not affect L-aspartate and L-glutamate transporter activity.

Sites not aligning to the query:

39 R→C: Completely abolishes L-aspartate and L-glutamate transporter activity. Retains appreciable H(+)-coupled sialic acid transporter activity.

Q5Q0U0 Sialin; H(+)/nitrate cotransporter; H(+)/sialic acid cotransporter; AST; Solute carrier family 17 (Anion/sugar transporter), member 5; Vesicular excitatory amino acid transporter; VEAT from Rattus norvegicus (Rat) (see 2 papers)
23% identity, 65% coverage: 65:348/438 of query aligns to 113:402/495 of Q5Q0U0

query
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Q5Q0U0

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G

A

M

A

V

T

G

G

P

A

A

V

V

L

F

L

L

I

V

L

A

Y

A

P

G

L

F

A

I

G

G

G

C

N

D

L

Y

L

S

V

L

S

A

M

V

A

A

L

F

L

L

T

T

L

I

S

S

I

T

S

T

M

L

K136 (≠ R88) mutation to E: Markedly decreases H(+)-coupled sialic acid transporter activity.
R168 (= R118) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
E171 (≠ L121) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-175.
G172 (= G122) mutation to C: Decreases protein levels and alters subcellular localization.
E175 (= E125) mutation to C: Decreases H(+)-coupled sialic acid transporter activity; when associated with C-171.
G176 (≠ A126) mutation to C: Decreases protein levels and alters subcellular localization.
F179 (≠ A129) mutation to C: Decreases the affinity and transport rate for D-glucuronate. Does not affect H(+)-coupled sialic acid transporter activity.
P180 (= P130) mutation to C: Decreases protein levels and alters subcellular localization.
H183 (≠ I133) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
W186 (≠ L136) mutation to C: Abolishes H(+)-coupled sialic acid transporter activity.
SSLKN 268:272 (≠ TELGT 225:229) mutation Missing: Abolishes H(+)-coupled sialic acid transporter activity.
P334 (= P289) mutation to R: Abolishes H(+)-coupled sialic acid transporter activity.
G371 (≠ P317) mutation to V: Remains in the endoplasmic reticulum.

Sites not aligning to the query:

22:23 Dileucine internalization motif; LL→AA: Targeted to plasma membrane; sialic acid uptake strongly activated at acidic pH.
39 R→C: Markedly decreases H(+)-coupled sialic acid transporter activity.

6e9nA E. Coli d-galactonate:proton symporter in the inward open form (see paper)
20% identity, 82% coverage: 25:384/438 of query aligns to 7:356/409 of 6e9nA

query
sites
6e9nA

L

L

P

V

L

M

L

I

F

F

I

I

G

T

Y

V

V

V

F

I

A

C

Y

Y

L

V

D

D

R

R

I

A

N

N

V

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

Q

Q

S

E

D

E

L

F

G

G

L

I

S

T

D

K

A

A

A

E

Y

M

G

G

A

Y

G

V

A

F

G

S

I

A

F

F

F

A

V

W

G

L

Y

Y

V

T

L

L

F

C

E

Q

L

I

P

P

S

G

T

G

L

W

M

F

L

L

P

D

R

R

I

V

G

G

A

S

R

R

K

V

T

T

F

Y

S

F

R

I

I

A

L

I

V

F

L

G

W

W

G

S

I

V

T

A

S

T

A

L

C

F

M

Q

L

G

F

F

V

A

R

T

D

G

V

L

P

M

T

S

F

L

Y

I

A

G

M

L

R

R

F

A

L

I

L

T

G

G

V

I

F

F

E

E

A

A

G

P

F

A

A
x
F

P

P

G

T

M

N

I

N

Y

R

Y

M

L

V

S

T

R

S

W

W

Y

F

G

P

P

E

S

H

R

E

M

R

A

A

R

S

A

A

I

V

A

G

I

F

V

-

F

Y

I

T

A

S

G

G

P

Q

M

F

G

V

G

G

I

L

L

-

G

-

P

-

I

-

S

-

A

A

W

F

L

L

I

T

T

P

T

L

F

L

A

I

G

W

V

I

G

Q

G

E

L

M

A

L

G

S

W

W

Q

H

W

W

M

V

F

F

L

I

I

V

E

T

G

G

L

G

P

I

C

G

V

I

F

I

L

W

G

S

V

L

L

I

M

W

Y

F

V

K

V

V

L

Y

C

-

D

Q

R

P

P

P

A

R

D

L

A

T

P

K

W

G

L

I

N

S

A

K

A

A

E

E

K

L

Q

D

L

Y

L

I

E

R

T

D

-

G

-

L

E

V

L

D

G

G

T

D

A

A

S

P

A

L

R

T

S

A

H

K

S

D

F

W

R

K

T

L

V

V

L

F

R

H

D

R

P

-

K

K

L

L

Y

I

V

G

L

V

A

Y

S

L

A

G

Y

Q

F

F

C

A

I

V

I

A

F

S

S

T

I

L

Y

W

A

F

M

F

S

L

F

T

W

W

L

F

P

P

A

N

V

Y

I

L

K

T

A

Q

L

E

G

K

V

G

N

I

D

T

T

A

Q

L

Q

K

L

A

G

G

W

F

Y

M

A

T

L

V

P

P

Y

F

V

L

G

A

A

A

A

F

L

V

G

G

M

V

Y

L

W

L

I

S

G

G

R

W

R

V

S

A

D

D

R

L

L

L

G

V

E

R

R

K

R

G

L

F

H

S

C

L

A

G

I

F

P

A

A

R

A

K

T

T

G

P

A

I

V

I

-

C

-

G

L

L

I

I

L

S

Y

T

P

C

L

I

A

M

G

G

G

A

N

N

L

Y

L

T

V

N

S

D

M

P

A

M

L

M

L

I

T

M

L

C

S

L

I

M

S

A

M

L

M

A

F

F

M

F

A

G

Y

N

-

G

-

F

-

A

T

S

V

I

L

T

W

W

A

S

M

L

P

V

S

S

E

S

H

-

I

-

K

-

G

-

E

-

A

-

G

-

I

L

A

A

L

P

I

M

N

R

T

L

I

I

G

G

L

L

S

T

G

G

binding D-gluconic acid: F111 (≠ A129)

Sites not aligning to the query:

binding D-gluconic acid: 359

P0AA76 D-galactonate transporter; D-galactonate/H(+) symporter from Escherichia coli (strain K12) (see paper)
25% identity, 38% coverage: 25:191/438 of query aligns to 18:177/430 of P0AA76

query
sites
P0AA76

L

L

P

V

L

M

L

I

F

F

I

I

G

T

Y

V

V

V

F

I

A

C

Y
|
Y

L

V

D
|
D

R
|
R

I

A

N

N

V

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

Q

Q

S

E

D

E

L

F

G

G

L

I

S

T

D

K

A

A

A

E

Y

M

G

G

A

Y

G

V

A

F

G

S

I

A

F

F

F

A

V

W

G

L

Y
|
Y

V

T

L

L

F

C

E

Q

L

I

P

P

S

G

T

G

L

W

M

F

L

L

P

D

R

R

I

V

G

G

A

S

R

R

K

V

T

T

F

Y

S

F

R

I

I

A

L

I

V

F

L

G

W

W

G

S

I

V

T

A

S

T

A

L

C

F

M

Q

L

G

F

F

V

A

R

T

D

G

V

L

P

M

T

S

F

L

Y

I

A

G

M

L

R

R

F

A

L

I

L

T

G

G

V

I

F

F

E
|
E

A

A

G

P

F

A

A

F

P

P

G

T

M

N

I

N

Y

R

Y

M

L

V

S

T

R

S

W

W

Y

F

G

P

P

E

S

H

R

E

M

R

A

A

R

S

A

A

I

V

A

G

I

F

V

-

F

Y

I

T

A

S

G

G

P

Q

M

F

G

V

G

G

I

L

L

-

G

-

P

-

I

-

S

-

A

A

W

F

L

L

I

T

T

P

T

L

F

L

A

I

G

W

V

I

G

Q

G

E

L

M

A

L

G

S

W

W

Q

H

W

W

M

V

F

F

L

I

I

V

E

T

G

G

Y29 (= Y36) binding
D31 (= D38) mutation to N: Loss of galactonate transport activity.
R32 (= R39) binding
Y64 (= Y71) binding
E118 (= E125) mutation to Q: Loss of galactonate transport activity.

Sites not aligning to the query:

358 binding

Q5EXK5 3-hydroxybenzoate transporter MhbT from Klebsiella oxytoca (see paper)
22% identity, 79% coverage: 84:428/438 of query aligns to 83:436/452 of Q5EXK5

query
sites
Q5EXK5

R

R

I

F

G

G

A

R

R

K

K

R

T

V

F

I

S

E

R

F

I

C

L

V

V

A

L

L

W

F

G

G

I

L

T

F

S

S

A

L

C

L

M

S

L

A

F

F

V

A

R

P

D

D

V

L

P

Q

T

M

F

L

Y

V

A

F

M

L

R

R

F

F

L

L

L

T

G

G

V

L

F

G

E

L

A

G

G

G

F

A

A

M

P

P

G

N

M

T

I

I

Y

T

Y

M

L

T

S

S

R

E

W

Y

Y

L

G

P

P

A

S

R

R

R

M

R

A

G

R

A

A

L

I

V

A

T

I

L

V

M

F

F

I

C

A

G

G

F

P

T

M

L

G

G

G

S

I

A

L

F

G

G

P

V

I

V

S

S

A

A

W

Q

L

L

I

V

T

P

T

-

F

-

A

-

G

-

V

-

G

-

L

L

A

I

G

G

W

W

Q

H

W

G

M

I

F

L

L

V

I

L

E

G

G

G

L

V

P

L

C

P

V

L

F

I

L

L

G

A

V

V

L

A

M

L

Y

V

V

P

V

L

L

L

C

P

D

E

R

S

P

P

A

R

D

W

A

Q

P

I

W

R

L

R

N

Q

A

L

A

P

E

Q

K

A

Q

T

L

I

L

A

E

R

T

T

E

V

L

G

G

A

T

I

A

T

S

G

A

E

R

R

S

Y

H

N

S

D

F

T

R

H

T

F

V

W

L

L

R

D

D

E

P

P

-

A

-

G

-

A

-

K

-

G

-

S

-

I

-

S

K

Q

L

L

Y

F

-

A

-

G

-

R

-

Q

-

L

-

A

-

I

-

T

-

L

V

M

L

L

A

W

S

V

A

V

Y

F

F

F

C

M

I

S

I

L

F

L

S

I

I

I

Y

Y

A

L

M

L

S

S

F

S

W

W

L

M

P

P

A

T

V

L

I

L

K

N

A

H

L

R

G

G

V

I

N

-

D

D

T

L

Q

Q

Q

H

L

A

G

S

W

W

Y

V

A

T

A

A

L

A

P

F

Y

Q

V

I

G

G

A

G

A

T

L

F

G

G

M

A

Y

L

W

L

I

L

G

G

R
x
V

R

L

S

M

D
|
D

R

R

L

F

G

N

E

P

R

Y

R

R

L

V

H

-

C

L

A

A

I

L

P

S

A

Y

A

G

T

L

G

G

A

A

V

V

L

C

L

I

I

V

L

M

Y

I

P

G

L

L

A

S

G

E

G

N

N

G

L

L

-

W

L

L

V

M

S

A

M

L

A

A

L

I

L

F

T

G

L

T

S

G

I

I

S

G

M

I

M

S

F

-

M

G

A

S

Y

Q

T

V

V

G

L

L

W

N

A

A

M

L

P

T

S

A

E

T

H

L

I

Y

K

P

G

T

E

Q

A

S

A

R

A

A

G

T

G

G

I

V

A

S

L

W

I

S

N

N

T

A

I

V

G

G

L

R

S

C

G

G

G

A

F

I

W

V

G

G

P

S

A

L

M

S

I

G

G

G

W

V

A

M

K

M

T

A

A

M

T

N

G

F

S

S

L

F

D

D

T

T

G

L

L

F

I

F

A

V

V

I

G

A

C

V

V

P

F

A

L

V

C

V

A

S

A

A

F

V

V

M

I

L

I

I

A

L

T

L

T

T

P

L

R

A

T

V

R

R

T

N

V311 (≠ R302) mutation to W: Loss of activity.
D314 (= D305) mutation to A: Loss of activity.

Sites not aligning to the query:

82 D→A: Loss of activity.

6e9oA E. Coli d-galactonate:proton symporter mutant e133q in the outward substrate-bound form (see paper)
24% identity, 38% coverage: 25:191/438 of query aligns to 10:169/393 of 6e9oA

query
sites
6e9oA

L

L

P

V

L

M

L

I

F

F

I

I

G

T

Y

V

V

V

F

I

A

C

Y
|
Y

L

V

D

D

R

R

I

A

N

N

V

L

G

A

F

V

A

A

K

S

L

A

Q

H

M

I

Q

Q

S

E

D

E

L

F

G

G

L

I

S

T

D

K

A

A

A

E

Y

M

G

G

A

Y

G

V

A

F

G

S

I

A

F

F

F

A

V

W

G

L

Y

Y

V

T

L

L

F

C

E

Q

L

I

P

P

S

G

T

G

L

W

M

F

L

L

P

D

R

R

I

V

G

G

A

S

R

R

K

V

T

T

F

Y

S

F

R

I

I

A

L

I

V

F

L

G

W

W

G

S

I

V

T

A

S

T

A

L

C

F

M

Q

L

G

F

F

V

A

R

T

D

G

V

L

P

M

T

S

F

L

Y

I

A

G

M

L

R

R

F

A

L

I

L

T

G

G

V

I

F

F

E

Q

A

A

G

P

F

A

A
x
F

P

P

G

T

M

N

I

N

Y

R

Y

M

L

V

S

T

R

S

W

W

Y

F

G

P

P

E

S

H

R

E

M

R

A

A

R

S

A

A

I

V

A

G

I

F

V

-

F

Y

I

T

A

S

G

G

P
x
Q

M

F

G

V

G

G

I

L

L

-

G

-

P

-

I

-

S

-

A

A

W

F

L

L

I

T

T

P

T

L

F

L

A

I

G

W

V

I

G

Q

G

E

L

M

A

L

G

S

W

W

Q

H

W

W

M

V

F

F

L

I

I

V

E

T

G

G

binding D-galactonic acid: Y21 (= Y36), F114 (≠ A129), Q141 (≠ P157)

Sites not aligning to the query:

binding D-galactonic acid: 228, 320, 343

Q9C0U9 Uncharacterized transporter PB1C11.03 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
22% identity, 71% coverage: 19:329/438 of query aligns to 93:415/570 of Q9C0U9

query
sites
Q9C0U9

K

K

L

L

N

K

W

W

R

K

L

L

L

Y

P

I

L

Y

L

L

F

L

I

L

G

-

Y

-

V

M

F

L

A

G

Y

F

L

L

D

D

R

M

-

M

I

L

N

F

V

I

G

G

F

K

A

A

K

T

L

L

Q

S

M

Y

Q

S

S

T

D

I

L

L

G

G

L

L

S

F

D

D

-

D

-

V

-

H

-

I

-

T

-

S

A

N

A

Q

Y

Y

G

N

A

N

G

L

A

N

G

T

I

L

F

F

F

Y

V

V

G

G

Y

Y

V

I

L

V

F

G

E

Q

L

F

P

P

S

G

T

H

L

Y

M

I

L

M

P

Q

R

T

I

F

G

P

A

L

R

G

K

K

T

F

F

V

S

G

R

L

I

V

L

T

V

F

L

S

W

W

G

S

I

V

T

I

S

V

A

F

C

L

M

H

L

C

F

C

V

A

R

Y

D

N

V

Y

P

G

T

G

F

L

Y

I

A

A

M

L

R

R

F

F

L

F

L

L

G

G

V

F

F

T

E

E

A

S

G

C

F

L

A

L

P

P

G

A

M

M

I

E

Y

A

Y

T

L

M

S

G

R

M

W

F

Y

F

G

T

P

H

S

Q

R

E

M

Q

A

A

R

F

A

L

I

Q

A

P

I

V

V

F

F

W

I

I

A

S

G

C

P

L

M

S

G

C

G

G

I

I

L

P

G

A

G

G

P

F

I

I

S

A

A

Y

W

G

L

L

I

-

T

-

T

E

F

F

A

V

G

-

V

T

G

K

G

S

L

I

A

A

G

P

W

W

Q

K

W

L

M

F

F

M

L

I

I

I

E

T

G

G

L

G

P

I

C

T

V

F

F

F

L

L

G

S

V

I

L

F

M

L

Y

F

V

F

V

Y

L

Y

C

P

D

D

R

N

P

P

A

S

D

K

A

A

P

R

W

F

L

L

N

T

A

D

A

E

E

E

K

K

Q

L

L

Y

L

T

E

I

T

D

E

R

L

V

G

R

T

K

A

S

S

T

A

R

-

G

-

I

-

E

-

N

-

K

-

I

-

F

R

K

S

K

H

H

S

Q

F

F

R

I

T

E

V

A

L

L

R

K

D

D

P

P

K

I

L

T

Y

W

V

L

L

F

A

T

S

F

A

A

Y

A

F

F

C

T

I

L

I

M

F

L

S

S

I

-

Y

N

A

N

M

L

S

A

F

Y

W

Q

L

Q

P

N

A

L

V

I

I

F

K

T

A

S

L

L

G

N

V

V

N

S

D

D

T

L

Q

N

Q

S

-

T

-

L

-

V

-

G

-

V

-

A

L

L

G

A

W

G

Y

Y

A

N

A

T

L

V

P

S

Y

A

V

I

G

I

A

A

A

T

L

F

G

A

M

M

Y

Y

W

L

I

I

G

P

R

N

R

Q

S

S

D

-

R

-

L

-

G

A

E

Y

R

H

R

A

L

M

H

F

C

W

A

M

I

L

P

P

A

S

A

I

T

T

G

G

A

G

V

I

L

A

L

F

I

V

L

A

Y

L

P

P

Sites not aligning to the query:

14 modified: Phosphoserine

Q03567 Uncharacterized transporter slc-17.2 from Caenorhabditis elegans (see paper)
22% identity, 69% coverage: 27:327/438 of query aligns to 21:354/493 of Q03567

query
sites
Q03567

L

V

L

M

F

F

I

F

G

G

Y

C

V

L

F

V

A

T

Y

Y

L

M

D

M

R

R

I

T

N

N

V

M

G

S

F

F

A

A

K

V

L

V

Q

C

M

M

-

V

-

N

-

E

-

N

Q

K

S

T

D

D

L

T

G

G

L

V

S

E

D

K

A

V

A

S

-

R

-

C

-

G

-

K

-

E

-

M

-

T

-

P

-

V

-

E

-

S

-
x
N

-

S

-

V

-

I

-

G

-

E

-

F

-

D

-

W

-

D

-

K

-

Q

-

T

Y

T

G

G

A

M

G

V

A

L

G

S

I

S

F

F

V

Y

G

G

Y

Y

V

I

L

G

F

S

E

Q

L

I

P

I

S

G

T

G

L

H

M

L

L

A

P

S

R

R

I

Y

G

G

A

G

R

K

K

-

T

-

F

-

S

-

R

R

I

V

L

V

V

F

L

V

W

T

G

I

I

L

T

G

S

S

A

A

C

L

M

L

L

T

F

L

V

L

R

N

D

P

V

V

-

A

-

R

P

T

T

S

F

E

Y

Y

A

A

M

L

R

A

F

I

L

L

-

R

-

A

L

I

G

G

V

F

F

L

E

Q

A

G

G

A

F

T

A

F

P

P

G

A

M

M

I

H

Y

T

Y

M

L

W

S

S

R

V

W

W

Y

G

G

P

P

P

S

L

R

E

M

L

A

S

R

V

A

L

I

T

A

G

I

V

V

T

F

Y

I

A

A

G

G

A

P

Q

M

I

G

G

G

N

I

V

L

I

G

V

G

L

P

P

I

L

S

S

A

G

W

F

L

L

I

C

T

E

T

-

F

-

A

-

G

-

V

Y

G

G

G

F

L

D

A

G

G

G

W

W

Q

P

W

S

M

I

F

F

L

Y

I

I

E

I

G

G

L

V

P

F

C

G

V

V

F

L

L

W

G

T

V

A

L

V

M

W

Y

W

V

Y

V

V

L

S

C

S

D

D

R

K

P

P

A

A

D

T

A

H

P

P

W

R

L

I

N

T

A

P

A

E

E

E

K

K

Q

Q

L

Y

L

I

-

V

-

T

-

A

-

V

E

E

T

A

E

S

L

M

G

G

T

K

A

D

S

T

A

G

R

K

-

V

-

P

S

S

H

T

S

P

F

W

R

I

T

K

V

I

L

L

R

T

D

S

P

P

K

A

L

V

Y

W

V

A

L

C

A

W

S

A

A

G

Y

H

F

F

C

A

I

G

I

D

F

W

S

G

I

A

Y

Y

A

T

M

M

S

L

F

V

W

S

L

L

P

P

A

S

V

F

I

L

K

K

-

D

A

V

L

L

G

G

V

L

N

N

D

-

T

L

Q

S

L

L

G

G

W

A

Y

V

A

A

A

S

L

I

P

P

Y

Y

V

I

G

A

A

Y

A

F

L

L

G

A

M

I

Y

N

W

A

I

G

G

G

R

V

R

L

S

A

D

D

R

T

L

L

G

R

E

S

R

K

R

G

L

I

H

L

C

S

A

T

I

L

P

N

A

T

A

R

T

R

G

A

A

A

V

M

L

L

L

V

I

A

L

L

N69 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

Q9JI12 Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6 from Rattus norvegicus (Rat) (see 2 papers)
20% identity, 58% coverage: 40:291/438 of query aligns to 104:357/582 of Q9JI12

query
sites
Q9JI12

I

I

N

H

V

R

G

G

F

G

A

K

K

V

L

I

Q

K

M

E

Q

K

S

A

D

K

L

F

G

N

L

W

S

D

D

P

A

E

A

T

Y

V

G

G

A

M

G

I

A
x
H

G

G

I

S

F

F

V

W

G

G

Y

Y

V

I

L

I

F

T

E

Q

L

I

P

P

S

G

T

G

L

Y

M

I

L

A

P

S

R

R

I

L

G

A

A

A

R

N

K

R

T

V

F

F

S

G

R

A

I

A

L

I

V

L

L

L

W

-

G

-

I

T

T

S

S

T

A

L

C

N

M

M

L

L

F

I

V

P

R

S

D

A

V

A

P

R

T

V

F

H

Y

Y

A

G

-

C

-

V

-

I

-

F

M

V

R
|
R

F

I

L

L

L

Q

G

G

V

L

F

V

E
|
E

A

G

G

V

F

T

A

Y

P

P

G

A

M

C

I

H

Y

G

Y

I

L

W

S

S

R

K

W

W

Y

A

G

P

P

P

S

L

R

E

M

R

A

S

R

R

A

L

I

A

A

T

I

T

V

S

F

F

I

C

A

G

G

S

P

Y

M

A

G

G

G

A

I

V

L

I

G

A

G

M

P

P

I

L

S

A

A

G

W

I

L

L

I

V

T

Q

T

-

F

-

A

-

G

-

V

-

G

-

L

Y

A

T

G

G

W

W

Q

S

W

S

M

V

F

F

L

Y

I

V

E

Y

G

G

L

S

P

F

C

G

V

M

F

V

L

W

G

Y

V

M

L

F

M

W

Y

L

V

L

V

V

L

S

C

Y

D

E

R

S

P

P

A

A

D

K

A

H

P

P

W

T

L

I

N

T

A

D

A

E

E

E

K

R

Q

R

L

Y

L

I

E

E

T

E

E

S

L

I

G

G

T

E

A

S

S

A

-

N

-

L

A

G

R

A

S

M

H

E

S

K

F

F

R

K

T

T

V

P

L

W

R

R

D

K

-

F

-

T

-

S

P

M

K

P

L

V

Y

Y

V

A

L

I

A

I

S

V

A

A

Y

N

F

F

C

C

I
x
R

I

S

F

W

S

T

I

F

Y

Y

A

L

M

L

S

L

F

I

W

S

L

Q

P

P

A

A

V

Y

I

F

K

E

A

E

L

V

G

F

V

G

N

F

D

E

T

I

Q

S

Q

K

L

V

G

G

W

M

Y

L

A

S

A

A

L

V

P

P

Y

H

V

L

H128 (≠ A64) mutation to A: Greatly lowers L-glutamate transport.
R184 (= R118) mutation R->A,E,K: Greatly lowers L-glutamate transport.
E191 (= E125) mutation to A: Greatly lowers L-glutamate transport.; mutation E->D,Q: Lowers L-glutamate transport.
R322 (≠ I256) mutation to A: Loss of L-glutamate release. Abolishes the chloride ion conductance.

Sites not aligning to the query:

88 R→A: Impairs synaptic transmission. Abolishes the chloride ion conductance.

6zguA Crystal structure of a mfs transporter with bound 3-(2-methylphenyl) propanoic acid at 2.41 angstroem resolution
23% identity, 87% coverage: 49:427/438 of query aligns to 32:393/404 of 6zguA

query
sites
6zguA

M

L

Q

N

S

A

D

E

L

F

G

G

L

W

S

S

D

R

A

A

A

E

Y

I

G

A

A

M

G

A

A

F

G

A

I

I

F

C

V

L

G

I

Y
x
F

V

G

L

L

F

M

E

T

L

F

P

P

S

A

T

G

L

R

M

L

L

S

P

D

R

K

I

M

G

G

A

P

R

R

K

K

T

V

F

V

S

M

R

T

I

G

L

G

V

V

L

L

W

L

G

A

I

I

T

G

S

F

A

I

C

L

M

S

L

G

F

F

V

I

R

Q

D

S

V

K

P

-

T

-

F

-

Y

Y

A

Q

M

L

R

Y

F

I

L

T

L

Y

G

G

V

V

F

I

E

-

A

A

G

G

F

F

A

G

P

G

G

G

M

M

I

I

Y
|
Y

Y

L

-

P

-

I

-

A

-

T

L

A

S

P

R

K

W

W

Y

W

G

-

P

P

S

D

R

R

M

R

A

A

R

L

A

A

I

T

A

G

I

F

V

A

F

V

I

V

A

G

G
x
L

P

G

M

L

G

G

G

S

I

F

L

L

G

M

G

G

P

P

I

L

S

A

A

T

W

Y

L

I

I

I

T

E

T

K

F

P

A

G

G

-

V

-

G

-

L

-

A

M

G

G

W

W

Q

R

W

Y

M

V

F

F

L

W

I

Y

E

C

G

G

L

V

P

A

C

M

V

G

F

I

L

M

G

A

V

L

L

I

M

A

Y

G

V

A

V

F

L

L

C

-

D

-

R

E

P

P

A

P

D

P

A

A

P

G

W

W

L

K

N

P

A

A

A

G

E

Y

K

T

Q

P

L

K

L

V

E

T

T

-

E

-

L

-

G

-

T

-

A

-

S

-

A

-

R

R

S

D

H

W

S

T

F

Y

R

E

T

E

V

A

L

K

R

G

D

D

P

T

K

K

L

F

Y

W

V

L

L

L

A

Y

S

L

A

A

Y

Y

F

F

C

C

I

G

I

S

F

F

S

A

I

G

Y

L

A

M

M

V

S

I

F

G

W

H

L

L

P

A

A

G

V

F

I

G

K

R

A

D

L

A

G

G

V

L

N

T

D

A

T

M

Q

A

L

A

G

G

W

A

Y

V

A

S

L

L

P

A

Y

F

V

S

G

N

A

A

L

T

G
x
R

M

I

Y

L

W

-

I

S

G

G

R

W

R

F

S

V

D

D

R

K

L

I

G

G

E

I

R

R

R

V

L

Y

H

F

C

A

A

A

I

L

P

F

A

A

A

L

T

Q

G

T

A

A

V

A

L

M

L

I

I

A

L

I

Y

F

P

Q

L

L

A

G

G

G

G

S

N

-

L

-

L

V

V

V

S

G

M

L

A

S

L

I

L

V

T

A

L

I

S

V

I

I

S

G

M

W

M

N

F

Y

M

G

A

A

Y

M

T
x
F

V

T

L

L

W

F

A

P

M

A

P

T

S

C

E

L

H

Q

I

F

K

Y

G

G

E

P

A

T

A

A

A

Q

G

G

G

S

I

N

-

Y

-

G

A

L

L

L

I

F

N

T

T

A

I
x
C

G

G

L

L

S

A

G

G

G

-

F

F

W

A

G

G

P

P

A

W

M

V

I

G

G

G

W

W

A

L

K

K

T

D

A

T

T

T

G

G

S

T

L

Y

D

Y

T

L

G

P

L

F

I

L

A

C

V

A

G

A

C

A

V

L

F

C

L

A

C

L

A

G

A

T

F

A

V

I

I

V

I

F

A

M

T

T

T

K

P

P

R

P

T

E

R

K

binding 3-(2-methylphenyl)propanoic acid: F54 (≠ Y71), Y113 (= Y134), L139 (≠ G156), R264 (≠ G296), F319 (≠ T354), C346 (≠ I379)

6zgtA Crystal structure of a mfs transporter with bound 2-naphthoic acid at 2.39 angstroem resolution
23% identity, 87% coverage: 49:427/438 of query aligns to 32:393/404 of 6zgtA

query
sites
6zgtA

M

L

Q

N

S

A

D

E

L

F

G

G

L

W

S

S

D

R

A

A

A

E

Y

I

G

A

A

M

G

A

A

F

G

A

I

I

F

C

F
x
C

V

L

G

I

Y
x
F

V

G

L

L

F

M

E

T

L

F

P

P

S

A

T

G

L

R

M

L

L

S

P

D

R

K

I

M

G

G

A

P

R

R

K

K

T

V

F

V

S

M

R

T

I

G

L

G

V

V

L

L

W

L

G

A

I

I

T

G

S

F

A

I

C

L

M

S

L

G

F

F

V

I

R

Q

D

S

V

K

P

-

T

-

F

-

Y

Y

A

Q

M

L

R

Y

F

I

L

T

L

Y

G

G

V

V

F

I

E

-

A

A

G

G

F

F

A

G

P

G

G

G

M

M

I

I

Y
|
Y

Y

L

-

P

-

I

-

A

-

T

L

A

S

P

R

K

W

W

Y

W

G

-

P

P

S

D

R

R

M

R

A

A

R

L

A

A

I

T

A

G

I

F

V

A

F

V

I

V

A

G

G
x
L

P

G

M

L

G

G

G

S

I

F

L

L

G

M

G

G

P

P

I

L

S

A

A

T

W

Y

L

I

I

I

T

E

T

K

F

P

A

G

G

-

V

-

G

-

L

-

A

M

G

G

W

W

Q

R

W

Y

M

V

F

F

L

W

I

Y

E

C

G

G

L

V

P

A

C

M

V

G

F

I

L

M

G

A

V

L

L

I

M

A

Y

G

V

A

V

F

L

L

C

-

D

-

R

E

P

P

A

P

D

P

A

A

P

G

W

W

L

K

N

P

A

A

A

G

E

Y

K

T

Q

P

L

K

L

V

E

T

T

-

E

-

L

-

G

-

T

-

A

-

S

-

A

-

R

R

S

D

H

W

S

T

F

Y

R

E

T

E

V

A

L

K

R

G

D

D

P

T

K

K

L

F

Y

W

V

L

L

L

A

Y

S

L

A

A

Y

Y

F

F

C

C

I

G

I

S

F

F

S

A

I

G

Y

L

A

M

M

V

S

I

F

G

W

H

L

L

P

A

A

G

V

F

I

G

K

R

A

D

L

A

G

G

V

L

N

T

D

A

T

M

Q

A

L

A

G

G

W

A

Y

V

A

S

L

L

P

A

Y

F

V

S

G

N

A

A

L

T

G
x
R

M

I

Y

L

W

-

I

S

G

G

R

W

R

F

S

V

D

D

R

K

L

I

G

G

E

I

R

R

R

V

L

Y

H

F

C

A

A

A

I

L

P

F

A

A

A

L

T

Q

G

T

A

A

V

A

L

M

L

I

I

A

L

I

Y

F

P

Q

L

L

A

G

G

G

G

S

N

-

L

-

L

V

V

V

S

G

M

L

A

S

L

I

L

V

T

A

L

I

S

V

I

I

S

G

M

W

M

N

F

Y

M

G

A

A

Y

M

T
x
F

V

T

L

L

W

F

A

P

M

A

P

T

S

C

E

L

H

Q

I

F

K

Y

G

G

E

P

A

T

A

A

A

Q

G

G

G

S

I

N

-

Y

-

G

A

L

L

L

I
x
F

N

T

T

A

I
x
C

G

G

L

L

S

A

G

G

G

-

F

F

W

A

G

G

P

P

A

W

M

V

I

G

G

G

W

W

A

L

K

K

T

D

A

T

T

T

G

G

S

T

L

Y

D

Y

T

L

G

P

L

F

I

L

A

C

V

A

G

A

C

A

V

L

F

C

L

A

C

L

A

G

A

T

F

A

V

I

I

V

I

F

A

M

T

T

T

K

P

P

R

P

T

E

R

K

binding naphthalene-2-carboxylic acid: C51 (≠ F68), F54 (≠ Y71), Y113 (= Y134), L139 (≠ G156), R264 (≠ G296), F319 (≠ T354), F343 (≠ I376), C346 (≠ I379)

6zgsA Crystal structure of a mfs transporter with bound 3-phenylpropanoic acid at 2.39 angstroem resolution
23% identity, 87% coverage: 49:427/438 of query aligns to 32:393/404 of 6zgsA

query
sites
6zgsA

M

L

Q

N

S

A

D

E

L

F

G

G

L

W

S

S

D

R

A

A

A

E

Y

I

G

A

A

M

G

A

A

F

G

A

I

I

F

C

V

L

G

I

Y
x
F

V

G

L

L

F

M

E

T

L

F

P

P

S

A

T

G

L

R

M

L

L

S

P

D

R

K

I

M

G

G

A

P

R

R

K

K

T

V

F

V

S

M

R

T

I

G

L

G

V

V

L

L

W

L

G

A

I

I

T

G

S

F

A

I

C

L

M

S

L

G

F

F

V

I

R

Q

D

S

V

K

P

-

T

-

F

-

Y

Y

A

Q

M

L

R

Y

F

I

L

T

L

Y

G

G

V

V

F

I

E

-

A

A

G

G

F

F

A

G

P

G

G

G

M

M

I

I

Y
|
Y

Y

L

-

P

-

I

-

A

-

T

L

A

S

P

R

K

W

W

Y

W

G

-

P

P

S

D

R

R

M

R

A

A

R

L

A

A

I

T

A

G

I

F

V

A

F

V

I

V

A

G

G
x
L

P

G

M

L

G

G

G

S

I

F

L

L

G

M

G

G

P

P

I

L

S

A

A

T

W

Y

L

I

I

I

T

E

T

K

F

P

A

G

G

-

V

-

G

-

L

-

A

M

G

G

W

W

Q

R

W

Y

M

V

F

F

L

W

I

Y

E

C

G

G

L

V

P

A

C

M

V

G

F

I

L

M

G

A

V

L

L

I

M

A

Y

G

V

A

V

F

L

L

C

-

D

-

R

E

P

P

A

P

D

P

A

A

P

G

W

W

L

K

N

P

A

A

A

G

E

Y

K

T

Q

P

L

K

L

V

E

T

T

-

E

-

L

-

G

-

T

-

A

-

S

-

A

-

R

R

S

D

H

W

S

T

F

Y

R

E

T

E

V

A

L

K

R

G

D

D

P

T

K

K

L

F

Y

W

V

L

L

L

A

Y

S

L

A

A

Y

Y

F

F

C

C

I

G

I

S

F

F

S

A

I

G

Y

L

A

M

M

V

S

I

F

G

W

H

L

L

P

A

A

G

V

F

I

G

K

R

A

D

L

A

G

G

V

L

N

T

D

A

T

M

Q

A

L

A

G

G

W

A

Y

V

A

S

L

L

P

A

Y

F

V

S

G

N

A

A

L

T

G
x
R

M

I

Y

L

W

-

I

S

G

G

R

W

R

F

S

V

D

D

R

K

L

I

G

G

E

I

R

R

R

V

L

Y

H

F

C

A

A

A

I

L

P

F

A

A

A

L

T

Q

G

T

A

A

V

A

L

M

L

I

I

A

L

I

Y

F

P

Q

L

L

A

G

G

G

G

S

N

-

L

-

L

V

V

V

S

G

M

L

A

S

L

I

L

V

T

A

L

I

S

V

I

I

S

G

M

W

M

N

F

Y

M

G

A

A

Y

M

T
x
F

V

T

L

L

W

F

A

P

M

A

P

T

S

C

E

L

H

Q

I

F

K

Y

G

G

E

P

A

T

A

A

A

Q

G

G

G

S

I

N

-

Y

-

G

A

L

L

L

I

F

N

T

T

A

I
x
C

G

G

L

L

S

A

G

G

G

-

F

F

W

A

G

G

P

P

A

W

M

V

I

G

G

G

W

W

A

L

K

K

T

D

A

T

T

T

G

G

S

T

L

Y

D

Y

T

L

G

P

L

F

I

L

A

C

V

A

G

A

C

A

V

L

F

C

L

A

C

L

A

G

A

T

F

A

V

I

I

V

I

F

A

M

T

T

T

K

P

P

R

P

T

E

R

K

binding hydrocinnamic acid: F54 (≠ Y71), Y113 (= Y134), L139 (≠ G156), R264 (≠ G296), F319 (≠ T354), C346 (≠ I379)

Q9Y2C5 Probable small intestine urate exporter; Solute carrier family 17 member 4 from Homo sapiens (Human) (see 2 papers)
24% identity, 41% coverage: 165:345/438 of query aligns to 194:402/497 of Q9Y2C5

query
sites
Q9Y2C5

P

P

I

L

S

E

A

R

W

S

L

Q

I

L

T

T

F

I

A

A

G

G

V

S

G

G

G

S

L

M

A

L

G

G

W

S

Q

F

W

I

M

V

F

L

L

L

I

A

E

G

G

G

L

L

P

L

C

C

-

Q

-

T

-

I

-

G

-

W

-

P

-

Y

-

V

-

F

-

Y

V

I

F

F

L

G

G

G

V

I

-

G

-

C

-

A

-

C

-

P

L

L

M

W

Y

F

V

P

V

L

L

I

C

Y

D

D

R

D

P

P

A

V

D

N

A

H

P

P

W

F

L

I

N

S

A

A

A

G

E

E

K

K

Q

R

L

Y

L

I

E

V

T

C

E

S

L

L

G

A

T

Q

A

Q

S

D

A

C

R

S

-

P

-

G

-

W

S

S

H

L

S

P

F

I

R

R

T

A

V

M

L

I

R

K

D

S

P

L

K

P

L

L

Y

W

V

A

L

I

A

L

S

V

A

S

Y

Y

F

F

C

C

I

E

I

Y

F

W

S

L

I

F

Y

Y

A

T

M

I

S

M

F

A

W

Y

L

T

P

P

A

T

V

Y

I

I

K

S

A

S

L

V

G

L

V

Q

N

A

D

N

T

L

Q

R

Q

D

L

S

G

G

W

I

Y

L

A

S

A

A

L

L

P

P

Y

F

V

V

-

V

-

G

-

C

-

I

-

C

-

I

-

L

G

G

A

G

A

L

L

L

G

A

M

D

Y

F

W

L

I

L

G

S

R

R

R

K

S

I

D

L

R

R

L

L

G

I

E

T

-

I

R

R

R

K

L

L

H

F

C

T

A
|
A

I

I

P

G

A

V

A

L

T

F

G

P

A

S

V

V

L

I

L

L

I

V

L

S

Y

L

P

P

L

W

A

V

G

R

G

S

N

S

L

H

L

S

V

M

S

T

M

M

A

T

L

F

L

L

T

V

L

L

S

S

A372 (= A315) to T: in dbSNP:rs11754288

Sites not aligning to the query:

66 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.
75 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-66 and A-90.
90 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→A: Loss of glycosylation and significant reduction in the transport of thyroid hormones T3 and T4; when associated with A-75 and A-90.

7t3nA R184q/e191q mutant of rat vesicular glutamate transporter 2 (vglut2)
19% identity, 62% coverage: 40:312/438 of query aligns to 46:320/452 of 7t3nA

query
sites
7t3nA

I

I

N

H

V

R

G

G

F

G

A

K

K

V

L

I

Q

K

M

E

Q

K

S

A

D

K

L

F

G

N

L

W

S

D

D

P

A

E

A

T

Y

V

G

G

A

M

G

I

A

H

G

G

I

S

F

F

V

W

G

G

Y
|
Y

V

I

L

I

F

T

E

Q

L

I

P

P

S

G

T

G

L

Y

M

I

L

A

P

S

R

R

I

L

G

A

A

A

R

N

K

R

T

V

F

F

S

G

R

A

I

A

L

I

V

L

L

L

W

-

G

-

I

T

T

S

S

T

A

L

C

N

M

M

L

L

F

I

V

P

R

S

D

A

V

A

P

R

T

V

F

H

Y

Y

A

G

M

C

R

V

F

I

L

F

L

V

G

Q

V

I

F

L

E

Q

A

-

G

G

F

L

A

V

P

Q

G

G

M

V

I

T

Y
|
Y

-

P

-

A

-

C

-

H

-

G

Y

I

L

W

S

S

R

K

W

W

Y

A

G

P

P

P

S

L

R
x
E

M

R

A

S

R

R

A

L

I

A

A

T

I

T

V

S

F

F

I

C

A
x
G

G

S

P
x
Y

M

A

G

G

G

A

I

V

L

I

G

A

G

M

P

P

I

L

S

A

A

G

W

I

L

L

I

V

T

Q

T

-

F

-

A

-

G

-

V

-

G

-

L

Y

A

T

G

G

W

W

Q

S

W

S

M

V

F

F

L

Y

I

V

E

Y

G

G

L

S

P

F

C

G

V

M

F

V

L

W

G

Y

V

M

L

F

M

W

Y

L

V

L

V

V

L

S

C

Y

D

E

R

S

P

P

A

A

D

K

A

H

P

P

W

T

L

I

N

T

A

D

A

E

E

E

K

R

Q

R

L

Y

L

I

E

E

T

E

E

S

L

I

G

G

T

E

A

S

S

A

-

N

-

L

A

G

R

A

S

M

H

E

S

K

F

F

R

K

T

T

V

P

L

W

R

R

D

K

-

F

-

T

-

S

P

M

K

P

L

V

Y

Y

V

A

L

I

A

I

S

V

A

A

Y

N

F

F

C

C

I
x
R

I

S

F

W

S

T

I
x
F

Y
|
Y

A

L

M

L

S

L

F

I

W

S

L

Q

P

P

A

A

V

Y

I

F

K

E

A

E

L

V

G

F

V

G

N

F

D

E

T

I

Q

S

Q

K

L

V

G

G

W

M

Y

L

A

S

A

A

L

V

P

P

Y

H

V

L

G

V

A

M

A

T

L

I

G

I

M

V

Y

P

W

I

I

G

G

G

R

Q

R

I

S

A

D

D

R

F

L

L

G

R

E

S

R

K

R

Q

L

I

binding (1s,3r)-1-aminocyclopentane-1,3-dicarboxylic acid: Y77 (= Y71), Y137 (= Y134), Y165 (≠ P157), R264 (≠ I256), F268 (≠ I260), Y269 (= Y261)
binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: E152 (≠ R144), G163 (≠ A155)

Sites not aligning to the query:

binding (2R)-2-(methoxymethyl)-4-{[(25R)-spirost-5-en-3beta-yl]oxy}butyl 4-O-alpha-D-glucopyranosyl-beta-D-glucopyranoside: 12, 13

A0LNN5 L-lactate transporter; SfMCT from Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) (see paper)
22% identity, 87% coverage: 49:427/438 of query aligns to 38:409/412 of A0LNN5

query
sites
A0LNN5

M

L

Q

N

S

A

D

E

L

F

G

G

L

W

S

S

D

R

A

A

A

E

Y

I

G

A

A

M

G

A

A

F

G

A

I

I

F

C

V

L

G

I

Y

F

V

G

L

L

F

M

E

T

L

F

P

P

S

A

T

G

L

R

M

L

L

S

P

D

R

K

I

M

G

G

A

P

R

R

K

K

T

V

F

V

S

M

R

T

I

G

L

G

V

V

L

L

W

L

G

A

I

I

T

G

S

F

A

I

C

L

M

S

L

G

F

F

V

I

R

Q

D

S

V

K

P

-

T

-

F

-

Y

Y

A

Q

M

L

R

Y

F

I

L

T

L

Y

G

G

V

V

F

I

E

-

A

A

G

G

F

F

A

G

P

G

G

G

M

M

I

I

Y
|
Y

Y

L

-

P

-

I

-

A

-

T

L

A

S

P

R

K

W

W

Y

W

G

-

P

P

S

D

R

R

M

R

A

A

R

L

A

A

I

T

A

G

I

F

V

A

F

V

I

V

A

G

G
x
L

P

G

M

L

G

G

G

S

I

F

L

L

G

M

G

G

P

P

I

L

S

A

A

T

W

Y

L

I

I

I

T

E

T

K

F

P

A

G

G

-

V

-

G

-

L

-

A

M

G

G

W

W

Q

R

W

Y

M

V

F

F

L

W

I

Y

E

C

G

G

L

V

P

A

C

M

V

G

F

I

L

M

G

A

V

L

L

I

M

A

Y

G

V

A

V

F

L

L

C

E

D

P

R

P

P

P

A

A

D

G

A

W

P

K

W

P

L

A

N

G

A

Y

A

T

E

P

K

P

Q

A

L

P

E

A

T

G

E

A

L

A

G

A

T

P

A

K

S

V

A

T

R

R

S

D

H

W

S

T

F

Y

R

E

T

E

V

A

L

K

R

G

D

D

P

T

K

K

L

F

Y

W

V

L

L

L

A

Y

S

L

A

A

Y

Y

F

F

C

C

I

G

I

S

F

F

S

A

I

G

Y

L

A

M

M

V

S

I

F

G

W
x
H

L

L

P

A

A

G

V

F

I

G

K
x
R

A
x
D

L

A

G

G

V

L

N

T

D

A

T

M

Q

A

L

A

G

G

W

A

Y

V

A

S

L

L

P

A

Y

F

V

S

G
x
N

A

A

L

T

G
x
R

M

I

Y

L

W

-

I

S

G

G

R

W

R

F

S

V

D

D

R

K

L

I

G

G

E

I

R

R

R

V

L

Y

H

F

C

A

A

A

I

L

P

F

A

A

A

L

T

Q

G

T

A

A

V

A

L

M

L

I

I

A

L

I

Y

F

P

Q

L

L

A

G

G

G

G

S

N

-

L

-

L

V

V

V

S

G

M

L

A

S

L

I

L

V

T

A

L

I

S

V

I

I

S

G

M

W

M

N

F
x
Y

M

G

A

A

Y

M

T
x
F

V

T

L

L

W

F

A

P

M

A

P

T

S

C

E

L

H

Q

I

F

K

Y

G

G

E

P

A

T

A

A

A

Q

G

G

G

S

I

N

-

Y

-

G

A

L

L

L

I
x
F

N

T

T

A

I
x
C

G

G

L

L

S

A

G

G

G

-

F

F

W

A

G

G

P

P

A

W

M

V

I

G

G

G

W

W

A

L

K
|
K

T
x
D

A

T

T

T

G

G

S

T

L
x
Y

D

Y

T

L

G

P

L

F

I

L

A

C

V

A

G

A

C

A

V

L

F

C

L

A

C

L

A

G

A

T

F

A

V

I

I

V

I

F

A

M

T

T

T

K

P

P

R

P

T

E

R

K

Y119 (= Y134) binding ; mutation Y->A,F: Loss of transport activity.
L145 (≠ G156) mutation to A: Strong decrease in transport activity.
H250 (≠ W266) mutation to A: Strong decrease in transport activity.; mutation to F: Loss of transport activity.
R256 (≠ K272) mutation to A: No change in transport activity.; mutation to D: Increases transport activity.
D257 (≠ A273) mutation to A: Loss of transport activity.
N276 (≠ G292) mutation to A: Loss of transport activity.
R280 (≠ G296) binding ; mutation to A: Abolishes L-lactate binding and L-lactate transport.
Y331 (≠ F350) mutation to A: Loss of transport activity.; mutation to F: No change in transport activity.
F335 (≠ T354) mutation to A: Increases transport activity.
F359 (≠ I376) mutation to A: Loss of transport activity.
C362 (≠ I379) mutation to A: Decrease in transport activity.
K377 (= K395) mutation K->A,D: No change in transport activity.
D378 (≠ T396) mutation to A: Loss of transport activity.
Y383 (≠ L401) mutation to A: Loss of transport activity.; mutation to F: Strong decrease in transport activity.

Sites not aligning to the query:

28 L→A: Loss of transport activity.

6g9xA Crystal structure of a mfs transporter at 2.54 angstroem resolution (see paper)
23% identity, 81% coverage: 73:427/438 of query aligns to 53:385/396 of 6g9xA

query
sites
6g9xA

L

L

F

M

E

T

L

F

P

P

S

A

T

G

L

R

M

L

L

S

P

D

R

K

I

M

G

G

A

P

R

R

K

K

T

V

F

V

S

M

R

T

I

G

L

G

V

V

L

L

W

L

G

A

I

I

T

G

S

F

A

I

C

L

M

S

L

G

F

F

V

I

R

Q

D

S

V

K

P

-

T

-

F

-

Y

Y

A

Q

M

L

R

Y

F

I

L

T

L

Y

G

G

V

V

F

I

E

-

A

A

G

G

F

F

A

G

P

G

G

G

M

M

I

I

Y
|
Y

Y

L

-

P

-

I

-

A

-

T

L

A

S

P

R

K

W

W

Y

W

G

-

P

P

S

D

R

R

M

R

A

A

R

L

A

A

I

T

A

G

I

F

V

A

F

V

I

V

A

G

G
x
L

P

G

M

L

G

G

G

S

I

F

L

L

G

M

G

G

P

P

I

L

S

A

A

T

W

Y

L

I

I

I

T

E

T

-

F

-

A

-

G

-

V

-

G

-

L

-

A

-

G

-

W

W

Q

R

W

Y

M

V

F

F

L

W

I

Y

E

C

G

G

L

V

P

A

C

M

V

G

F

I

L

M

G

A

V

L

L

I

M

A

Y

G

V

A

V

F

L

L

C

E

D

P

R

P

P

P

A

A

D

G

A

W

P

K

W

P

L

A

N

G

A

A

E

P

K

K

Q

-

L

-

E

-

T

-

E

-

L

-

G

-

T

-

A

-

S

V

A

T

R

R

S

D

H

W

S

T

F

Y

R

E

T

E

V

A

L

K

R

G

D

D

P

T

K

K

L

F

Y

W

V

L

L

L

A

Y

S

L

A

A

Y

Y

F

F

C

C

I

G

I

S

F

F

S

A

I

G

Y

L

A

M

M

V

S

I

F

G

W

H

L

L

P

A

A

G

V

F

I

G

K

R

A

D

L

A

G

G

V

L

N

T

D

A

T

M

Q

A

L

A

G

G

W

A

Y

V

A

S

L

L

P

A

Y

F

V

S

G

N

A

A

L

T

G
x
R

M

I

Y

L

W

-

I

S

G

G

R

W

R

F

S

V

D

D

R

K

L

I

G

G

E

I

R

R

R

V

L

Y

H

F

C

A

A

A

I

L

P

F

A

A

A

L

T

Q

G

T

A

A

V

A

L

M

L

I

I

A

L

I

Y

F

P

Q

L

L

A

G

G

G

G

S

N

-

L

-

L

V

V

V

S

G

M

L

A

S

L

I

L

V

T

A

L

I

S

V

I

I

S

G

M

W

M

N

F
x
Y

M

G

A

A

Y

M

T
x
F

V

T

L

L

W

F

A

P

M

A

P

T

S

C

E

L

H

Q

I

F

K

Y

G

G

E

P

A

T

A

A

A

Q

G

G

G

S

I

N

-

Y

-

G

A

L

L

L

I

F

N

T

T

A

I
x
C

G

G

L

L

S

A

G

G

G

-

F

F

W

A

G

G

P

P

A

W

M

V

I

G

G

G

W

W

A

L

K

K

T

D

A

T

T

T

G

G

S

T

L

Y

D

Y

T

L

G

P

L

F

I

L

A

C

V

A

G

A

C

A

V

L

F

C

L

A

C

L

A

G

A

T

F

A

V

I

I

V

I

F

A

M

T

T

T

K

P

P

R

P

T

E

R

K

binding 2-sulfanylbenzoic acid: Y110 (= Y134), L136 (≠ G156), R256 (≠ G296), Y307 (≠ F350), F311 (≠ T354), C338 (≠ I379)

Q8NLB7 Gentisate transporter from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025) (see paper)
24% identity, 66% coverage: 23:311/438 of query aligns to 39:318/444 of Q8NLB7

query
sites
Q8NLB7

R

R

L

R

L

L

P

A

L

A

L

V

F

L

I

I

G

G

Y

W

V

F

F

F

A

V

Y

I

L

F

D
|
D

R

G

I

Y

N
x
D

V

L

-

I

-

V

-

Y

G

G

F

T

A

V

K

Q

L

S

Q

A

M

L

Q

A

S

K

D

E

L

W

G

N

L

L

S

S

D

S

A

A

A

T

Y

L

G

G

A

T

G

I

A

G

G

S

I

T

F

A

F

F

V

F

G

G

Y

M

V

A

L

I

F

G

E

A

L

V

P

F

S

I

T

G

L

R

M

L

L

S

P

D

R
|
R

I

V

G

G

A

R

R

K

K

A

-

A

T

V

F

I

S

G

R

S

I

V

L

L

V

I

L

L

W

S

G

V

I

F

T

T

S

M

A

L

C

C

M

A

L

-

F

F

V

A

R

P

D

N

V

P

P

W

T

V

F

F

Y

G

A

A

M

F

R

R

F

F

L

I

L

A

G

G

V

L

F

G

E

L

A

G

G

G

F

L

A

V

P

P

G

S

M

V

I

N

Y

A

Y

M

L

T

S

S

R

D

W

L

Y

V

G

-

P

P

S

R

R

K

M

T

A

M

R

S

A

A

I

W

A

A

I

T

V

V

F

M

I

M

A

S

G

G

P

V

M

P

G

-

I

-

L

I

G

G

P

S

I

I

S

A

A

A

W

V

L

L

I

A

T

L

T

V

F

V

A

V

G

P

V

S

G

S

G

E

L

E

A

W

G

G

W

W

Q

R

W

F

M

M

F

F

L

L

I

I

E

A

G

L

L

I

P

P

C

L

V

V

F

V

-

G

L

L

G

P

V

I

L

A

M

M

Y

K

V

V

V

I

L

P

C

S

D

D

R

K

P

A

A

I

D

K

A

A

P

-

W

-

L

-

N

-

A

-

E

-

K

-

Q

-

L

-

E

D

T

H

E

D

L

I

G

R

T

E

A

G

S

H

A

D

R

E

S

P

H

A

S

G

F

F

R

K

T

D

V

L

L

L

R

V

D

D

P

R

K

Y

L

R

Y

W

V

I

-

S

-

I

-

W

L

F

A

A

S

L

A

A

Y

T

F

F

C

V

I

T

I

L

F

L

S

A

I

W

Y

Y

A

G

M

L

S

G

F

T

W

W

L

L

P

P

A

R

V

L

I

M

K

E

A

T

L

A

G

G

V

Y

N

E

D

F

T

G

Q

H

Q

A

L

L

G

M

W

F

Y

T

A

L

A

A

L

L

P

-

Y

N

V

L

G

G

A

A

A

V

L

I

G

G

M

S

Y

V

W

V

I

T

G

A

R
x
W

R

A

S

G

D
|
D

R
|
R

L

F

G

G

E

P

R
x
I

R

R

D54 (= D38) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
D57 (≠ N41) mutation to A: Loss of transport activity.; mutation to E: Retains 50% of its transport activity.
R103 (= R84) mutation to A: Loss of transport activity.
W309 (≠ R302) mutation to V: Loss of transport activity.
D312 (= D305) mutation to A: Loss of transport activity.
R313 (= R306) mutation to A: Loss of transport activity.
I317 (≠ R310) mutation I->H,Y: Loss of transport activity.

Sites not aligning to the query:

386 R→A: Loss of transport activity.

6zgrA Crystal structure of a mfs transporter with bound 1- hydroxynaphthalene-2-carboxylic acid at 2.67 angstroem resolution
24% identity, 79% coverage: 73:416/438 of query aligns to 54:370/399 of 6zgrA

query
sites
6zgrA

L

L

F

M

E

T

L

F

P

P

S

A

T

G

L

R

M

L

L

S

P

D

R

K

I

M

G

G

A

P

R

R

K

K

T

V

F

V

S

M

R

T

I

G

L

G

V

V

L

L

W

L

G

A

I

I

T

G

S

F

A

I

C

L

M

S

L

G

F

F

V

I

R

Q

D

S

V

K

P

-

T

-

F

-

Y

Y

A

Q

M

L

R

Y

F

I

L

T

L

Y

G

G

V

V

F

I

E

-

A

A

G

G

F

F

A

G

P

G

G

G

M

M

I

I

Y
|
Y

Y

L

-

P

-

I

-

A

-

T

L

A

S

P

R

K

W

W

Y

W

G

-

P

P

S

D

R

R

M

R

A

A

R

L

A

A

I

T

A

G

I

F

V

A

F

V

I

V

A

G

G
x
L

P

G

M

L

G

G

G

S

I

F

L

L

G

M

G

G

P

P

I

L

S

A

A

T

W

Y

L

I

I

I

T

E

T

-

F

-

A

-

G

-

V

-

G

-

L

-

A

-

G

-

W

W

Q

R

W

Y

M

V

F

F

L

W

I

Y

E

C

G

G

L

V

P

A

C

M

V

G

F

I

L

M

G

A

V

L

L

I

M

A

Y

G

V

A

V

F

L

L

C

-

D

-

R

E

P

P

A

P

D

P

A

A

P

G

W

W

L

K

N

P

A

A

A

G

E

Y

K

T

Q

A

L

A

L

P

E

K

T

V

E

T

L

-

G

-

T

-

A

-

S

-

A

-

R

R

S

D

H

W

S

T

F

Y

R

E

T

E

V

A

L

K

R

G

D

D

P

T

K

K

L

F

Y

W

V

L

L

L

A

Y

S

L

A

A

Y

Y

F

F

C

C

I

G

I

S

F

F

S

A

I

G

Y

L

A

M

M

V

S

I

F

G

W

H

L

L

P

A

A

G

V

F

I

G

K

R

A

D

L

A

G

G

V

L

N

T

D

A

T

M

Q

A

L

A

G

G

W

A

Y

V

A

S

L

L

P

A

Y

F

V

S

G

N

A

A

L

T

G
x
R

M

I

Y

L

W

-

I

S

G

G

R

W

R

F

S

V

D

D

R

K

L

I

G

G

E

I

R

R

R

V

L

Y

H

F

C

A

A

A

I

L

P

F

A

A

A

L

T

Q

G

T

A

A

V

A

L

M

L

I

I

A

L

I

Y

F

P

Q

L

L

A

G

G

G

G

S

N

-

L

-

L

V

V

V

S

G

M

L

A

S

L

I

L

V

T

A

L

I

S

V

I

I

S

G

M

W

M

N

F
x
Y

M

G

A

A

Y

M

T
x
F

V

T

L

L

W

F

A

P

M

A

P

T

S

C

E

L

H

Q

I

F

K

Y

G

G

E

P

A

T

A

A

A

Q

G

G

G

S

I

N

-

Y

-

G

A

L

L

L

I
x
F

N

T

T

A

I

C

G

G

L

L

S

A

G

G

G

-

F

F

W

A

G

G

P

P

A

W

M

V

I

G

G

G

W

W

A

L

K

K

T

D

A

T

T

T

G

G

S

T

L

Y

D

Y

T

L

G

-

L

-

I

-

A

-

V

-

G

-

C

-

V

P

F

F

L

L

C

C

A

A

binding 1-hydroxynaphthalene-2-carboxylic acid: Y111 (= Y134), L137 (≠ G156), R259 (≠ G296), Y310 (≠ F350), F314 (≠ T354), F338 (≠ I376)

Sites not aligning to the query:

binding 1-hydroxynaphthalene-2-carboxylic acid: 52

Query Sequence

>GFF2124 PS417_10835 major facilitator transporter
MPTAPAPATNEAIAALYHKLNWRLLPLLFIGYVFAYLDRINVGFAKLQMQSDLGLSDAAY
GAGAGIFFVGYVLFELPSTLMLPRIGARKTFSRILVLWGITSACMLFVRDVPTFYAMRFL
LGVFEAGFAPGMIYYLSRWYGPSRMARAIAIVFIAGPMGGILGGPISAWLITTFAGVGGL
AGWQWMFLIEGLPCVFLGVLMYVVLCDRPADAPWLNAAEKQLLETELGTASARSHSFRTV
LRDPKLYVLASAYFCIIFSIYAMSFWLPAVIKALGVNDTQQLGWYAALPYVGAALGMYWI
GRRSDRLGERRLHCAIPAATGAVLLILYPLAGGNLLVSMALLTLSISMMFMAYTVLWAMP
SEHIKGEAAAGGIALINTIGLSGGFWGPAMIGWAKTATGSLDTGLIAVGCVFLCAAFVII
ATTPRTRTGQTGDYIRSH

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory