SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2134 FitnessBrowser__Phaeo:GFF2134 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
36% identity, 95% coverage: 4:528/551 of query aligns to 6:450/458 of 8b7sA

query
sites
8b7sA

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binding flavin-adenine dinucleotide: G11 (= G9), G13 (= G11), S14 (= S12), A15 (= A13), E35 (= E32), A36 (≠ H33), W47 (= W59), P65 (= P77), G67 (= G79), V180 (≠ A219), A214 (= A253), G215 (≠ S254), A218 (≠ N257), T270 (= T330), Y391 (= Y467), A424 (≠ S502), I435 (≠ L513), N436 (= N514)

5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
35% identity, 96% coverage: 4:531/551 of query aligns to 25:572/578 of 5nccA

query
sites
5nccA

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active site: R347 (≠ A319), L420 (≠ V381), I421 (≠ G382), S507 (≠ A466), A509 (≠ H468), G552 (= G511), Q553 (≠ N512)
binding flavin-adenine dinucleotide: G30 (= G9), G32 (= G11), T33 (≠ S12), A34 (= A13), L53 (≠ I31), E54 (= E32), A55 (≠ H33), F74 (≠ N53), W80 (= W59), A98 (≠ P77), G100 (= G79), G105 (= G84), S106 (= S85), N110 (= N89), A111 (≠ G90), T112 (≠ M91), L113 (≠ V92), V238 (≠ R220), A278 (= A253), H282 (≠ N257), L286 (= L261), N508 (≠ Y467), Q553 (≠ N512), T554 (≠ L513), G555 (≠ N514), V558 (≠ S517)

3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
37% identity, 96% coverage: 4:532/551 of query aligns to 15:530/530 of 3ljpA

query
sites
3ljpA

D

D

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Y

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W

N

D

E

D

D

R

A

G

G

H

-

G

-

G

P

D

D

P

A

D

P

W

H

R

H

G

G

T

D

D

S

G

G

P

P

L

V

H

H

V

L

T

M

R

N

G

V

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P

R

P

D

K

N

D

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P

L

T

H

G

A

V

A

A

F

L

V

L

K

D

A

A

G

C

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E

Q

Q

A

A

G

G

Y

I

P

P

V

R

S

A

K

K

D

-

Y

F

N

N

G

T

E

G

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Q

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V

G

V

F

N

G

G

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M

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M

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I

Y

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R

-

A

-

D

G

G

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R

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S

S

S

A

S

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N

V

A

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Y

Y

L

I

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A

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K

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E

N

N

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A
|
A

R

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F

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-

A

D

D

G

R

R

R

A

C

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T

G

G

V

V

E

D

V

I

E

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R

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G

S

-

A

-

F

G

G

K

H

I

T

E

H

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R

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L

R

T

A

A

N

R

A

N

E

E

V

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I

V

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L

A

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A
x
T

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G

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A

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I

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x
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K

L

L

L

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M

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S

G

G

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I

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A

A

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A

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L

A

A

E

E

H

H

G

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I

I

D

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V

V

V

L

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D

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V

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L

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D

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Y

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F

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K

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-

L

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Y

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I

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D

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G

G

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T

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G

G

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F

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V

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I

I

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F

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A

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D

D

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E

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L

N

Q

G

D

F

Y

I

I

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R

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K

H

T

V

H

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N

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T

A
|
A

Y
|
Y

H
|
H

P

P

C

V

G

G

T

T

C

V

K

R

M

M

G

G

A

A

V

V

E

E

D

D

P

E

M

M

A

S

V

P

V

L

D

D

P

P

E

E

C

L

R

R

V

V

I

K

G

G

V

V

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T

G

G

L

L

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V

A

A

D
|
D

S
x
A

S

S

I

V

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M

P

P

R

E

I

H

T

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N

T

G
x
V

N
|
N

L
x
P

N
|
N

G

I

P

T

S
x
V

I

M

M

M

T

I

G

G

E

E

K

R

A

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A

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D

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I

I

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A

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R

active site: I333 (≠ S340), P377 (≠ V381), N378 (≠ G382), A464 (= A466), H466 (= H468), V509 (≠ G511), N510 (= N512)
binding dihydroflavine-adenine dinucleotide: G22 (= G11), S23 (= S12), E44 (= E32), A45 (≠ H33), W71 (= W59), R89 (= R78), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ G90), I103 (≠ V92), A232 (= A219), T269 (≠ A253), D273 (≠ N257), Y465 (= Y467), H466 (= H468), D499 (= D501), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ S517)

A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
35% identity, 96% coverage: 4:531/551 of query aligns to 85:639/654 of A0A248QE08

query
sites
A0A248QE08

D

D

Y

Y

V

I

I

L

V

V

G

G

A

G

G

G

S
x
T

A
|
A

G

A

C

C

A

V

M

L

A

A

Y

N

R

R

L

L

S

S

E

A

A

D

G

G

-

S

K

K

S

R

V

V

L

L

V

V

I

L

E
|
E

H

A

G

G

G

P

T

D

D

N

A

T

G

S

P

R

F

D

I

V

Q

K

M

I

P

P

G

A

A

A

L

I

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T

Y

R

P

-

M

L

N

F

M

R

S

S

M

P

Y

L

D

D

W

W

G

N

Y

L

K

F

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S

Q

E

P

L

E

Q

P

E

H

Q

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L

G

A

G

E

R

R

E

Q

L

I

V

Y

C

M

P

A

R

R

G

G

K

R

V
x
L

V

L

G

G

G

G

S
|
S

S

S

S

A

I

T

N
|
N

G
x
A

M
x
T

V
x
L

Y

Y

V

H

R

R

G

G

H

A

A

A

G

G

D

D

Y

Y

N

D

H

A

W

W

A

-

E

-

S

-

G

G

A

V

A

E

G

G

W

W

S

S

Y

S

A

E

D

D

V

V

L

L

P

S

Y

W

F

F

K

V

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Q

M

A

E

E

T

T

W

N

D

A

D

D

R

F

G

G

H

P

G

G

G

A

D

-

P

-

D

-

W

Y

R

H

G

G

T

S

D

G

G

G

P

P

L

M

H

R

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V

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-

R

E

G

N

P

P

R

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-

Y

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D

N

N

K

P

Q

L

L

H

H

A

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A

A

F

F

V

F

K

K

A

A

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A

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E

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E

A

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G

G

Y

L

P

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S

N

K

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D

D

Y

F

N

N

-

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H

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A

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G

P

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M

F

E

Q

M

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T

M

V

Q

Y

D

K

K

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G

Q

T

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W

A

S

D

A

M

A

Y

N

R

A

Q

Y

Y

L

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P

A

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R

D

R

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N

C

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-

G

A

A

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A

A
x
V

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K

V

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V

N

I

I

E

D

D

Q

G

A

-

A

-

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-

A

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A

A

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L

G

G

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V

E

E

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T

G

D

G

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P

I

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-

G

E

E

V

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I

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A

A

N

E

-

L

-

A

-

P

-

G

A

G

E

E

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I

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M

A

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A

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A

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E

E

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F

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D

P

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D

N

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P

A

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G

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G

Q

Q

N

N

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Q

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A

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A

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Q

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D

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A

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I

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H

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A

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G

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R

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G

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-

A

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C

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A

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D

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A

G

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Q

Y

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H
x
R

F

F

L

V

P

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-

G

I

M

A

A

V

L

R

D

Y

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D

D

G

G

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A

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A

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x
Y

-

V

-

R

-

F

-

A

-

K

-

F

-

Q

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G

-

L

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P

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x
Q

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A

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G

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E

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T

G

L

L

A

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S

A

A

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D

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N

F

D

A

A

P

P

P

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K

I

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L

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F

P

N

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Y

Y

M

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-

T

T

E

D

K

K

D

D

W

G

E

A

D

D

F

L

-

A

-

T

-

L

R

R

K

K

C

G

I

I

R

H

L

W

T

A

R

R

E

D

V

V

F

A

A

R

Q

S

D

S

A

A

M

L

K

S

P

E

F

Y

V

L

K

D

H

G

E

E

I

L

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F

P

P

G

G

D

S

A

G

L

V

Q

V

S

S

D

D

E

D

E

Q

L

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N

D

G

E

F

Y

I

I

R

R

E

R

H

S

V

I

E

H

S

S

A

S

Y

N

H

A

P

I

C

T

G

G

T

T

C

C

K

K

M

M

G

G

A

N

V

A

E

G

D

D

P

S

M

S

A

S

V

V

V

V

D

D

P

N

E

Q

C

L

R

R

V

V

I

H

G

G

V

V

E

E

G

G

L

L

R

R

V

V

A

V

D

D

S

A

S

S

I

V

F

V

P

P

R

K

I

I

T

P

N

G

G

G

N

Q

L

T

N
x
G

G

A

P

P

S

V

I

V

M

M

T

I

G

A

E

E

K

R

A

A

S

A

D

A

H

L

I

L

L

T

G

G

R

K

TA 93:94 (≠ SA 12:13) binding FAD
E114 (= E32) binding FAD
L162 (≠ V81) binding FAD
S166 (= S85) binding FAD
NATL 170:173 (≠ NGMV 89:92) binding FAD
V298 (≠ A219) binding FAD
C432 (≠ F338) binding hexadecanoate
R451 (≠ H358) binding hexadecanoate
Y466 (vs. gap) binding hexadecanoate
Q486 (≠ H380) binding hexadecanoate
G622 (≠ N514) binding FAD

6yrvAAA structure of fap after illumination at 100k (see paper)
35% identity, 96% coverage: 4:531/551 of query aligns to 9:563/573 of 6yrvAAA

query
sites
6yrvAAA

D

D

Y

Y

V

I

I

L

V

V

G
|
G

A

G

G
|
G

S
x
T

A
|
A

G

A

C

C

A

V

M

L

A

A

Y

N

R

R

L

L

S

S

E

A

A

D

G

G

-

S

K

K

S

R

V

V

L

L

V

V

I
x
L

E
|
E

H
x
A

G

G

G

P

T

D

D

N

A

T

G

S

P

R

F

D

I

V

Q

K

M

I

P

P

G

A

A

A

L

I

S

T

Y

R

P

-

M

L

N
x
F

M

R

S

S

M

P

Y

L

D

D

W
|
W

G

N

Y

L

K

F

S

S

Q

E

P

L

E

Q

P

E

H

Q

L

L

G

A

G

E

R

R

E

Q

L

I

V

Y

C

M

P
x
A

R

R

G

G

K

R

V

L

V

L

G

G

G
|
G

S
|
S

S

S

S

A

I

T

N
|
N

G
x
A

M
x
T

V
x
L

Y

Y

V

H

R

R

G

G

H

A

A

A

G

G

D

D

Y

Y

N

D

H

A

W

W

A

-

E

-

S

-

G

G

A

V

A

E

G

G

W

W

S

S

Y

S

A

E

D

D

V

V

L

L

P

S

Y

W

F

F

K

V

R

Q

M

A

E

E

T

T

W

N

D

A

D

D

R

F

G

G

H

P

G

G

G

A

D

-

P

-

D

-

W

Y

R

H

G

G

T

S

D

G

G

G

P

P

L

M

H

R

V

V

T

-

R

E

G

N

P

P

R

R

-

Y

-

T

D

N

N

K

P

Q

L

L

H

H

A

T

A

A

F

F

V

F

K

K

A

A

G

A

E

E

Q

E

A

V

G

G

Y

L

P

T

V

P

S

N

K

S

D

D

Y

F

N

N

-

D

-

W

G

S

E

H

Q

D

Q

H

E

A

G

G

F

Y

G

G

P

T

M

F

E

Q

M

V

T
x
M

V

Q

Y

D

K

K

G

G

Q

T

R

R

W

A

S

D

A

M

A

Y

N

R

A

Q

Y

Y

L

L

K

K

P

P

A

V

L

L

K

G

R

R

D

R

N

N

C

L

E

Q

M

V

I

L

R

T

-

G

A

A

L

A

A
x
V

R

T

K

K

V

V

V

N

I

I

E

D

D

Q

G

A

-

A

-

G

-

K

-

A

R

Q

A

A

V

L

G

G

V

V

E

E

V

F

E

S

R

T

G

D

G

G

K

P

I

T

-

G

E

E

V

R

I

L

R

S

A

A

N

E

-

L

-

A

-

P

-

G

A

G

E

E

V

V

I

I

L

M

A
x
C

A
|
A

S
x
G

S

A

L

V

N
x
H

S

T

P

P

K

F

L

L

L

L

M

K

L

H

S

S

G

G

I

V

G

G

P

P

A

S

K

A

H

E

L

L

A

K

E

E

H

F

G

G

I

I

D

P

V

V

V

V

V

S

D

N

R

L

P

A

G

G

V

V

G

G

Q

Q

N

N

L

L

Q

Q

D

D

H

Q

L

P

E

A

F

C

Y

L

F

T

Q

A

F

A

A

P

S

V

K

K

Q

E

P

K

I

Y

T

D

-

G

-

I

-

A

-

I

-

S

-

D

-

H

L

I

F

Y

K

N

Y

E

W

K

N

G

L

Q

F

I

G

R

K

K

A

R

L

A

V

I

G

A

A

S

Q

Y

W

L

L

L

F

G

T

G

K

R

T

G

G

G

L

L

G

-

A

T

S

S

N

T

Q

G

F

C

E

D

S

R

A

G

A

A

F

F

I

V

R

R

S

T

D

-

K

A

G

G

V

Q

D

A

Y

L

P

P

D

D

I

L

Q

Q

Y

V

H
x
R

F

F

L
x
V

P

P

-
x
G

I
x
M

A

A

V

L

R

D

Y

P

D

D

G
|
G

Q

V

A

S

A
x
T

-
x
Y

-

V

-

R

-
x
F

-

A

-

K

-

F

-

Q

A

S

E

Q

G

G

-

L

-

K

-

W

-

P

H

S

G

G

F

I

Q
x
T

A

M

H
x
Q

V

L

G

I

P

A

M

C

R

R

S

P

D

Q

S

S

R

T

G

G

E

S

V

V

T

G

L

L

A

K

S

S

A

A

D

D

P

P

N

F

D

A

A

P

P

P

K

K

I

L

L

S

F

P

N

G

Y

Y

M

L

S

-

T

T

E

D

K

K

D

D

W

G

E

A

D

D

F

L

-

A

-

T

-

L

R

R

K

K

C

G

I

I

R

H

L

W

T

A

R

R

E

D

V

V

F

A

A

R

Q

S

D

S

A

A

M

L

K

S

P

E

F

Y

V

L

K

D

H

G

E

E

I

L

Q

F

P

P

G

G

D

S

A

G

L

V

Q

V

S

S

D

D

E

D

E

Q

L

I

N

D

G

E

F

Y

I

I

R

R

E

R

H

S

V

I

E

H

S

S

A

S

Y
x
N

H

A

P

I

C

T

G

G

T

T

C

C

K

K

M

M

G

G

A

N

V

A

E

G

D

D

P

S

M

S

A

S

V

V

V

V

D

D

P

N

E

Q

C

L

R

R

V

V

I

H

G

G

V

V

E

E

G

G

L

L

R

R

V

V

A

V

D

D

S
x
A

S

S

I

V

F

V

P

P

R

K

I

I

T

P

N

G

G

G

N
x
Q

L
x
T

N
x
G

G

A

P

P

S

V

I

V

M

M

T

I

G

A

E

E

K

R

A

A

S

A

D

A

H

L

I

L

L

T

G

G

R

K

binding carbon dioxide: R375 (≠ H358), N499 (≠ Y467)
binding flavin-adenine dinucleotide: G14 (= G9), G16 (= G11), T17 (≠ S12), A18 (= A13), L37 (≠ I31), E38 (= E32), A39 (≠ H33), F58 (≠ N53), W64 (= W59), A82 (≠ P77), G89 (= G84), S90 (= S85), N94 (= N89), A95 (≠ G90), T96 (≠ M91), L97 (≠ V92), M191 (≠ T189), V222 (≠ A219), C264 (≠ A252), A265 (= A253), G266 (≠ S254), H269 (≠ N257), N499 (≠ Y467), A534 (≠ S502), Q544 (≠ N512), T545 (≠ L513), G546 (≠ N514)
binding heptadecane: V377 (≠ L360), G379 (vs. gap), M380 (≠ I362), G386 (= G368), T389 (≠ A371), Y390 (vs. gap), F393 (vs. gap), T408 (≠ Q378), Q410 (≠ H380)

4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
37% identity, 96% coverage: 4:532/551 of query aligns to 15:530/532 of 4mjwA

query
sites
4mjwA

D

D

Y

Y

V

I

I

V

V

V

G
|
G

A

G

G
|
G

S
|
S

A

A

G

G

C

A

A

A

M

V

A

A

Y

A

R

R

L

L

S

S

E

E

-

D

A

P

G

A

K

V

S

S

V

V

L

A

V

L

I

V

E
|
E

H
x
A

G

G

G

P

T

D

D

D

A

R

G

G

P

-

F

-

I

-

Q

-

M

V

P

P

G

E

A

V

L

L

S

Q

Y

L

P

D

M

R

N

W

M

M

S

E

M

L

-

L

-

E

-

S

-

G

Y

Y

D

D

W
|
W

G

D

Y

Y

K

P

S

I

Q

E

P

P

E

Q

P

E

H

N

L

-

G

G

G

N

R

S

E

F

L

M

V

R

C

H

P

A

R
|
R

G
x
A

K

K

V

V

V

M

G

G

G
|
G

S
x
C

S

S

S

S

I
x
H

N
|
N

G
x
S

M

C

V
x
I

Y

A

V

F

R

W

G

A

H

P

A

R

G

E

D

D

Y

L

N

D

H

E

W

W

-

E

A

A

E

K

S

Y

G

G

A

A

A

T

G

G

W

W

S

N

Y

A

A

E

D

A

V

A

L

W

P

P

Y

L

F

Y

K

K

R

R

M

L

E

E

T

T

W

N

D

E

D

D

R

A

G

G

H

-

G

-

G

P

D

D

P

A

D

P

W

H

R

H

G

G

T

D

D

S

G

G

P

P

L

V

H

H

V

L

T

M

R

N

G

V

P

P

R

P

D

K

N

D

P

P

L

T

H

G

A

V

A

A

F

L

V

L

K

D

A

A

G

C

E

E

Q

Q

A

A

G

G

Y

I

P

P

V

R

S

A

K

K

D

-

Y

F

N

N

G

T

E

G

Q

T

Q

T

E

V

G

V

F

N

G

G

P

A

M

N

E

F

M

F

T

Q

V

I

Y

N

K

R

-

R

-

A

-

D

G

G

Q

T

R

R

W

S

S

S

A

S

A

S

N

V

A

S

Y

Y

L

I

K

H

P

P

A

I

L

V

K

E

R

Q

D

E

N

N

C

F

E

T

M

L

I

L

R

T

A

G

L

L

-
x
R

A
|
A

R

R

K

Q

V

L

V

V

I

F

E

D

-

A

D

D

G

R

R

R

A

C

V

T

G

G

V

V

E

D

V

I

E

V

R

D

G

S

-

A

-

F

G

G

K

H

I

T

E

H

V

R

I

L

R

T

A

A

N

R

A

N

E

E

V

V

I

V

L

L

A

S

A
x
T

S
x
G

S

A

L

I

N
x
D

S

T

P

P

K

K

L

L

L

L

M

M

L

L

S

S

G

G

I

I

G

G

P

P

A

A

K

A

H

H

L

L

A

A

E

E

H

H

G

G

I

I

D

E

V

V

V

L

V

V

D

D

R

S

P

P

G

G

V

V

G

G

Q

E

N

H

L

L

Q

Q

D

D

H

H

L

P

E

E

F

G

Y

V

F

V

Q

Q

F

F

A

E

S

A

K

K

Q

Q

P

P

I

M

T

V

L

A

F

-

K

-

Y

-

W

-

N

-

L

-

F

-

G

-

K

-

A

-

L

-

V

E

G

S

A

T

Q

Q

W

W

L

-

F

-

T

-

K

-

T

-

G

-

L

-

G

-

A

-

S

-

N

-

Q

-

F

W

E

E

S
x
I

A

G

A

I

F

F

I

T

R

P

S

T

D

E

K

D

G

G

V

L

D

D

Y

R

P

P

D

D

I

L

Q

M

Y

M

H

H

F

Y

L

G

P

-

I

-

A

S

V

V

R

P

Y

F

D

D

G

M

Q

N

A

T

A

L

A

R

E

H

G

G

H

Y

-

P

-

T

-

T

-

E

-

N

G

G

F

F

Q

S

A

L

H

T

V
x
P

G
x
N

P

V

M

T

R

H

S

A

D

R

S

S

R

R

G

G

E

T

V

V

T

R

L

L

A

R

S

S

A

R

D

D

P

F

N

R

D

D

A

K

P

P

K

M

I

V

L

D

F

P

N

R

Y

Y

M

F

S

-

T

T

E

D

K

P

D

E

W

G

E

H

D

D

F

M

R

R

K

V

C

M

-

V

-

A

-

G

I

I

R

R

L

K

T

A

R

R

E

E

V

I

F

A

A

A

Q

Q

D

P

A

A

M

M

K

A

P

E

F

W

V

T

K

G

H

R

E

E

I

L

Q

S

P

P

G

G

D

V

A

E

L

A

Q

Q

S

T

D

D

E

E

E

E

L

L

N

Q

G

D

F

Y

I

I

R

R

E

K

H

T

V

H

E

N

S

T

A
x
V

Y
|
Y

H
|
H

P

P

C

V

G

G

T

T

C

V

K

R

M

M

G

G

A

A

V

V

E

E

D

D

P

E

M

M

A

S

V

P

V

L

D

D

P

P

E

E

C

L

R

R

V

V

I

K

G

G

V

V

E

T

G

G

L

L

R

R

V

V

A

A

D

D

S
x
A

S

S

I

V

F

M

P

P

R

E

I

H

T

V

N

T

G
x
V

N
|
N

L
x
P

N
|
N

G

I

P

T

S
x
V

I

M

M

M

T

I

G

G

E

E

K

R

A

C

S

A

D

D

H

L

I

I

L

R

G

S

R

A

R

R

active site: I333 (≠ S340), P377 (≠ V381), N378 (≠ G382), V464 (≠ A466), H466 (= H468), V509 (≠ G511), N510 (= N512)
binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), S23 (= S12), E44 (= E32), A45 (≠ H33), W71 (= W59), R89 (= R78), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ G90), I103 (≠ V92), R231 (vs. gap), A232 (= A219), T269 (≠ A253), G270 (≠ S254), D273 (≠ N257), Y465 (= Y467), H466 (= H468), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ S517)

2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
37% identity, 95% coverage: 4:528/551 of query aligns to 15:526/527 of 2jbvA

query
sites
2jbvA

D

D

Y

Y

V

I

I

V

V

V

G

G

A

G

G
|
G

S
|
S

A

A

G

G

C

A

A

A

M

V

A

A

Y

A

R

R

L

L

S

S

E

E

-

D

A

P

G

A

K

V

S

S

V

V

L

A

V

L

I

V

E
|
E

H
x
A

G

G

G

P

T

D

D

D

A

R

G

G

P

-

F

-

I

-

Q

-

M

V

P

P

G

E

A

V

L

L

S

Q

Y

L

P

D

M

R

N

W

M

M

S

E

M

L

-

L

-

E

-

S

-

G

Y

Y

D

D

W
|
W

G

D

Y

Y

K

P

S

I

Q

E

P

P

E

Q

P

E

H

N

L

-

G

G

G

N

R

S

E

F

L

M

V

R

C

H

P

A

R

R

G
x
A

K

K

V

V

V

M

G

G

G
|
G

S
x
C

S

S

S

S

I
x
H

N
|
N

G
x
S

M

C

V
x
I

Y

A

V

F

R

W

G

A

H

P

A

R

G

E

D

D

Y

L

N

D

H

E

W

W

-

E

A

A

E

K

S

Y

G

G

A

A

A

T

G

G

W

W

S

N

Y

A

A

E

D

A

V

A

L

W

P

P

Y

L

F

Y

K

K

R

R

M

L

E

E

T

T

W

N

D

E

D

D

R

A

G

G

H

-

G

-

G

P

D

D

P

A

D

P

W

H

R

H

G

G

T

D

D

S

G

G

P

P

L

V

H

H

V

L

T

M

R

N

G

V

P

P

R

P

D

K

N

D

P

P

L

T

H

G

A

V

A

A

F

L

V

L

K

D

A

A

G

C

E

E

Q

Q

A

A

G

G

Y

I

P

P

V

R

S

A

K

K

D

-

Y

F

N

N

G

T

E

G

Q

T

Q

T

E

V

G

V

F

N

G

G

P

A

M

N

E

F

M

F

T

Q

V

I

Y

N

K

R

-

R

-

A

-

D

G

G

Q

T

R

R

W

S

S

S

A

S

A

S

N

V

A

S

Y

Y

L

I

K

H

P

P

A

I

L

V

K

E

R

Q

D

E

N

N

C

F

E

T

M

L

I

L

R

T

A

G

L

L

-
x
R

A
|
A

R

R

K

Q

V

L

V

V

I

F

E

D

-

A

D

D

G

R

R

R

A

C

V

T

G

G

V

V

E

D

V

I

E

V

R

D

G

S

-

A

-

F

G

G

K

H

I

T

E

H

V

R

I

L

R

T

A

A

N

R

A

N

E

E

V

V

I

V

L

L

A

S

A
x
T

S
x
G

S

A

L

I

N
x
D

S

T

P

P

K

K

L

L

L

L

M

M

L

L

S

S

G

G

I

I

G

G

P

P

A

A

K

A

H

H

L

L

A

A

E

E

H

H

G

G

I

I

D

E

V

V

V

L

V

V

D

D

R

S

P

P

G

G

V

V

G

G

Q

E

N

H

L

L

Q

Q

D

D

H

H

L

P

E

E

F

G

Y

V

F

V

Q

Q

F

F

A

E

S

A

K

K

Q

Q

P

P

I

M

T

-

L

-

F

-

K

-

Y

-

W

-

N

-

L

-

F

-

G

-

K

-

A

V

L

A

V

E

G

S

A

T

Q

Q

W

W

L

-

F

-

T

-

K

-

T

-

G

-

L

-

G

-

A

-

S

-

N

-

Q

-

F

W

E

E

S
x
I

A

G

A

I

F

F

I

T

R

P

S

T

D

E

K

D

G

G

V

L

D

D

Y

R

P

P

D

D

I

L

Q

M

Y

M

H

H

F

Y

L

G

P

-

I

-

A

S

V

V

R

P

Y

F

D

D

G

M

Q

N

A

T

A

L

A

R

E

H

G

G

H

Y

-

P

-

T

-

T

-

E

-

N

G

G

F

F

Q

S

A

L

H

T

V
x
P

G
x
N

P

V

M

T

R

H

S

A

D

R

S

S

R

R

G

G

E

T

V

V

T

R

L

L

A

R

S

S

A

R

D

D

P

F

N

R

D

D

A

K

P

P

K

M

I

V

L

D

F

P

N

R

Y

Y

M

F

S

-

T

T

E

D

K

P

D

E

W

G

E

H

D

D

F

M

R

R

K

V

C

M

-

V

-

A

-

G

I

I

R

R

L

K

T

A

R

R

E

E

V

I

F

A

A

A

Q

Q

D

P

A

A

M

M

K

A

P

E

F

W

V

T

K

G

H

R

E

E

I

L

Q

S

P

P

G

G

D

V

A

E

L

A

Q

Q

S

T

D

D

E

E

E

E

L

L

N

Q

G

D

F

Y

I

I

R

R

E

K

H

T

V

H

E

N

S

T

A
x
V

Y
|
Y

H
|
H

P

P

C

V

G

G

T

T

C

V

K

R

M

M

G

G

A

A

V

V

E

E

D

D

P

E

M

M

A

S

V

P

V

L

D

D

P

P

E

E

C

L

R

R

V

V

I

K

G

G

V

V

E

T

G

G

L

L

R

R

V

V

A

A

D
|
D

S
x
A

S

S

I

V

F

M

P

P

R

E

I

H

T

V

N

T

G
x
V

N
|
N

L
x
P

N
|
N

G

I

P

T

S
x
V

I

M

M

M

T

I

G

G

E

E

K

R

A

C

S

A

D

D

H

L

I

I

active site: I333 (≠ S340), P377 (≠ V381), N378 (≠ G382), V464 (≠ A466), H466 (= H468), V509 (≠ G511), N510 (= N512)
binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G11), S23 (= S12), E44 (= E32), A45 (≠ H33), W71 (= W59), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ G90), I103 (≠ V92), R231 (vs. gap), A232 (= A219), T269 (≠ A253), G270 (≠ S254), D273 (≠ N257), V464 (≠ A466), Y465 (= Y467), H466 (= H468), D499 (= D501), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ S517)

4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
32% identity, 95% coverage: 2:523/551 of query aligns to 1:499/508 of 4ha6A

query
sites
4ha6A

N

N

A

C

D

D

Y

I

V

V

I

I

V

V

G
|
G

A

G

G
|
G

S
|
S

A
|
A

G

G

C

S

A

L

M

L

A

A

Y

A

R

R

L

L

S

S

E

E

A

D

G

P

K

D

S

S

-

R

V

V

L

L

V

L

I

I

E
|
E

H
x
A

G

G

G

E

T

E

D

P

A

T

G

D

P

P

F

D

I

I

Q

W

M

N

P

P

G

A

A

A

L

W

S

P

Y

A

P

L

M

Q

N

G

M

R

S

S

M

-

Y

Y

D

D

W
|
W

G

D

Y

Y

K

R

S

T

Q

E

P

A

E

Q

P

A

H

G

L

T

G

A

G

G

R

R

E

A

L

H

V

H

C

W

P

A

R
|
R

G
|
G

K

R

V

L

V

I

G

G

G
|
G

S
|
S

S

S

S

C

I
x
L

N
x
H

G
x
A

M
|
M

V
x
G

Y

Y

V

M

R

R

G

G

H

H

A

P

G

S

D

D

Y

F

N

Q

H

A

W

W

A

V

E

D

-

A

S

S

G

G

A

D

A

R

G

R

W

W

S

G

Y

W

A

D

D

E

V

L

L

L

P

P

Y

V

F

F

K

Q

R

A

M

I

E

E

T

-

W

-

D

D

D

H

R

P

G

L

H

G

G

G

G

D

D

G

P

I

D

H

W

G

R

K

G

G

T

G

D

P

G

L

P

P

L

I

H

H

V

L

T

-

R

-

G

-

P

P

R

A

D

D

-

E

-

V

N

S

P

P

L

L

H

A

A

R

A

A

F

F

V

I

K

E

A

A

G

G

E

A

Q

S

A

L

G

G

Y

L

P

P

V

R

S

L

K

E

D

G

Y

H

N

N

G

S

E

G

Q

E

Q

M

E

I

G

G

F

V

G

T

P

P

M

N

E

S

M

L

T

N

V

I

Y

R

K

D

G

G

Q

R

R

R

W

V

S

T

A

A

A

A

N

D

A

A

Y

W

L

L

K

T

P

K

A

A

L

V

K

R

-

G

R

R

D

K

N

N

C

L

E

T

M

I

I

L

R

T

-

G

A

S

L

R

A
x
V

R

R

K

R

V

L

V

K

I

L

E

E

D

G

G

N

R

Q

A

V

V

R

G

S

V

L

E

E

V

V

E

V

-

G

R

R

G

Q

G

G

K

S

I

A

E

E

V

V

I

F

R

-

A

-

N

A

A

D

E

Q

V

I

I

V

L

L

A

C

A
|
A

S
x
G

S

A

L

L

N
x
E

S

S

P

P

K

A

L

L

L

L

M

M

L

R

S

S

G

G

I

I

G

G

P

P

A

H

K

D

H

V

L

L

A

D

E

A

H

A

G

G

I

V

D

G

V

C

V

L

V

I

D

D

R

M

P

P

G

D

V

I

G

G

Q

R

N

N

L

L

Q

Q

D

D

H

H

L

L

E

L

F

G

Y

A

F

G

Q

N

-

L

F

Y

A

A

S

A

K

R

Q

K

P

P

I

V

T

P

L

P

F

S

K

R

Y

L

W

-

N

-

L

-

F

-

G

-

K

-

A

-

L

-

V

-

G

-

A

-

Q

-

W

-

L

-

F

-

T

-

K

-

T

-

G

-

L

-

G

-

A

-

S

-

N

Q

Q

H

F
x
S

E

E

S

S

A

M

A

A

F

Y

I

M

R

R

S

A

D

D

K

S

-

F

-

T

G

A

V

A

D

G

Y

Q

P

P

D

E

I

I

Q

V

Y

V

-

G

-

C

H

G

F

V

L

A

P

P

I

I

A

-

V

V

R

S

Y

E

D

S

G

F

Q

P

A

A

A

P

A

A

E

A

G

G

H

S

G

A

F

Y

Q

S

A

L

H

L

V
x
F

G
|
G

P

I

M

T

R

H

S

P

D

T

S

S

R

R

G

G

E

S

V

V

T

R

L

I

A

S

S

G

A

P

D

E

P

L

N

G

D

D

A

R

P

L

K

I

I

I

L

D

F

P

N

A

Y

Y

M

L

S

Q

T

T

E

G

K

R

D

D

W

R

E

E

D

R

F

F

R

R

K

R

C

A

I

L

R

E

L

A

T

S

R

R

E

T

V

I

F

G

A

H

Q

R

D

D

A

E

M

L

K

A

P

G

F

W

V

R

K

E

H

R

E

E

I

L

Q

L

P

P

G

G

D

T

A

P

L

-

Q

N

S

S

D

A

E

A

E

E

L

M

N

D

G

D

F

F

I

I

R

A

E

R

H

S

V

V

E

I

S

T

A
x
H

Y
x
H

H
|
H

P

P

C

C

G

G

T

T

C

C

K

R

M

M

G

G

A

-

V

-

E

K

D

D

P

P

M

D

A

A

V

V

V

V

D

D

P

A

E

N

C

L

R

R

V

L

I

K

G

A

V

L

E

D

G

N

L

L

R

F

V

V

A

V

D

D

S
x
A

S

S

I

I

F

M