SitesBLAST
Comparing GFF2134 FitnessBrowser__Phaeo:GFF2134 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
8b7sA Crystal structure of the chloramphenicol-inactivating oxidoreductase from novosphingobium sp (see paper)
36% identity, 95% coverage: 4:528/551 of query aligns to 6:450/458 of 8b7sA
- binding flavin-adenine dinucleotide: G11 (= G9), G13 (= G11), S14 (= S12), A15 (= A13), E35 (= E32), A36 (≠ H33), W47 (= W59), P65 (= P77), G67 (= G79), V180 (≠ A219), A214 (= A253), G215 (≠ S254), A218 (≠ N257), T270 (= T330), Y391 (= Y467), A424 (≠ S502), I435 (≠ L513), N436 (= N514)
5nccA Structure of fatty acid photodecarboxylase in complex with fad and palmitic acid (see paper)
35% identity, 96% coverage: 4:531/551 of query aligns to 25:572/578 of 5nccA
- active site: R347 (≠ A319), L420 (≠ V381), I421 (≠ G382), S507 (≠ A466), A509 (≠ H468), G552 (= G511), Q553 (≠ N512)
- binding flavin-adenine dinucleotide: G30 (= G9), G32 (= G11), T33 (≠ S12), A34 (= A13), L53 (≠ I31), E54 (= E32), A55 (≠ H33), F74 (≠ N53), W80 (= W59), A98 (≠ P77), G100 (= G79), G105 (= G84), S106 (= S85), N110 (= N89), A111 (≠ G90), T112 (≠ M91), L113 (≠ V92), V238 (≠ R220), A278 (= A253), H282 (≠ N257), L286 (= L261), N508 (≠ Y467), Q553 (≠ N512), T554 (≠ L513), G555 (≠ N514), V558 (≠ S517)
3ljpA Crystal structure of choline oxidase v464a mutant (see paper)
37% identity, 96% coverage: 4:532/551 of query aligns to 15:530/530 of 3ljpA
- active site: I333 (≠ S340), P377 (≠ V381), N378 (≠ G382), A464 (= A466), H466 (= H468), V509 (≠ G511), N510 (= N512)
- binding dihydroflavine-adenine dinucleotide: G22 (= G11), S23 (= S12), E44 (= E32), A45 (≠ H33), W71 (= W59), R89 (= R78), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ G90), I103 (≠ V92), A232 (= A219), T269 (≠ A253), D273 (≠ N257), Y465 (= Y467), H466 (= H468), D499 (= D501), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ S517)
A0A248QE08 Fatty acid photodecarboxylase, chloroplastic; CvFAP; EC 4.1.1.106 from Chlorella variabilis (Green alga) (see paper)
35% identity, 96% coverage: 4:531/551 of query aligns to 85:639/654 of A0A248QE08
- TA 93:94 (≠ SA 12:13) binding
- E114 (= E32) binding
- L162 (≠ V81) binding
- S166 (= S85) binding
- NATL 170:173 (≠ NGMV 89:92) binding
- V298 (≠ A219) binding
- C432 (≠ F338) binding
- R451 (≠ H358) binding
- Y466 (vs. gap) binding
- Q486 (≠ H380) binding
- G622 (≠ N514) binding
6yrvAAA structure of fap after illumination at 100k (see paper)
35% identity, 96% coverage: 4:531/551 of query aligns to 9:563/573 of 6yrvAAA
- binding carbon dioxide: R375 (≠ H358), N499 (≠ Y467)
- binding flavin-adenine dinucleotide: G14 (= G9), G16 (= G11), T17 (≠ S12), A18 (= A13), L37 (≠ I31), E38 (= E32), A39 (≠ H33), F58 (≠ N53), W64 (= W59), A82 (≠ P77), G89 (= G84), S90 (= S85), N94 (= N89), A95 (≠ G90), T96 (≠ M91), L97 (≠ V92), M191 (≠ T189), V222 (≠ A219), C264 (≠ A252), A265 (= A253), G266 (≠ S254), H269 (≠ N257), N499 (≠ Y467), A534 (≠ S502), Q544 (≠ N512), T545 (≠ L513), G546 (≠ N514)
- binding heptadecane: V377 (≠ L360), G379 (vs. gap), M380 (≠ I362), G386 (= G368), T389 (≠ A371), Y390 (vs. gap), F393 (vs. gap), T408 (≠ Q378), Q410 (≠ H380)
4mjwA Crystal structure of choline oxidase in complex with the reaction product glycine betaine (see paper)
37% identity, 96% coverage: 4:532/551 of query aligns to 15:530/532 of 4mjwA
- active site: I333 (≠ S340), P377 (≠ V381), N378 (≠ G382), V464 (≠ A466), H466 (= H468), V509 (≠ G511), N510 (= N512)
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), S23 (= S12), E44 (= E32), A45 (≠ H33), W71 (= W59), R89 (= R78), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ G90), I103 (≠ V92), R231 (vs. gap), A232 (= A219), T269 (≠ A253), G270 (≠ S254), D273 (≠ N257), Y465 (= Y467), H466 (= H468), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ S517)
2jbvA Crystal structure of choline oxidase reveals insights into the catalytic mechanism (see paper)
37% identity, 95% coverage: 4:528/551 of query aligns to 15:526/527 of 2jbvA
- active site: I333 (≠ S340), P377 (≠ V381), N378 (≠ G382), V464 (≠ A466), H466 (= H468), V509 (≠ G511), N510 (= N512)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-5-[(4aS,10aR)-7,8-dimethyl-2,4-dioxo-1,3,4,4a,5,10a-hexahydrobenzo[g]pteridin-10(2H)-yl]-2,3,4-trihydroxypentyl dihydrogen diphosphate: G22 (= G11), S23 (= S12), E44 (= E32), A45 (≠ H33), W71 (= W59), A90 (≠ G79), G95 (= G84), C96 (≠ S85), H99 (≠ I88), N100 (= N89), S101 (≠ G90), I103 (≠ V92), R231 (vs. gap), A232 (= A219), T269 (≠ A253), G270 (≠ S254), D273 (≠ N257), V464 (≠ A466), Y465 (= Y467), H466 (= H468), D499 (= D501), A500 (≠ S502), N510 (= N512), P511 (≠ L513), N512 (= N514), V515 (≠ S517)
4ha6A Crystal structure of pyridoxine 4-oxidase - pyridoxamine complex (see paper)
32% identity, 95% coverage: 2:523/551 of query aligns to 1:499/508 of 4ha6A
- active site: F360 (≠ V381), G361 (= G382), H444 (≠ A466), H446 (= H468), G487 (= G511), P488 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E32), A33 (≠ H33), W58 (= W59), R77 (= R78), G78 (= G79), G83 (= G84), S84 (= S85), L87 (≠ I88), H88 (≠ N89), A89 (≠ G90), M90 (= M91), G91 (≠ V92), V218 (≠ A219), A251 (= A253), G252 (≠ S254), E255 (≠ N257), H445 (≠ Y467), A478 (≠ S502), P488 (≠ N512), I489 (≠ L513), H490 (≠ N514)
- binding 4-(aminomethyl)-5-(hydroxymethyl)-2-methylpyridin-3-ol: A89 (≠ G90), S314 (≠ F338), H444 (≠ A466), H446 (= H468)
3t37A Crystal structure of pyridoxine 4-oxidase from mesorbium loti
32% identity, 95% coverage: 2:523/551 of query aligns to 1:499/509 of 3t37A
- active site: F360 (≠ V381), G361 (= G382), H444 (≠ A466), H446 (= H468), G487 (= G511), P488 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), S11 (= S12), A12 (= A13), E32 (= E32), A33 (≠ H33), W58 (= W59), R77 (= R78), G78 (= G79), R79 (≠ K80), G83 (= G84), S84 (= S85), H88 (≠ N89), A89 (≠ G90), G91 (≠ V92), R217 (≠ L218), V218 (≠ A219), A251 (= A253), E255 (≠ N257), H445 (≠ Y467), A478 (≠ S502), P488 (≠ N512), I489 (≠ L513), H490 (≠ N514)
8bxlB Patulin synthase from penicillium expansum
30% identity, 96% coverage: 4:530/551 of query aligns to 15:588/590 of 8bxlB
- binding flavin-adenine dinucleotide: G20 (= G9), G22 (= G11), T23 (≠ S12), A24 (= A13), E44 (= E32), A45 (≠ H33), W80 (= W59), G100 (= G79), G105 (= G84), S106 (= S85), R109 (≠ I88), N110 (= N89), Y111 (≠ G90), A113 (≠ V92), L253 (= L218), A254 (= A219), A288 (= A253), Q292 (≠ N257), F525 (≠ Y467), D559 (= D501), A560 (≠ S502), H570 (≠ N512), P571 (≠ L513), Q572 (≠ N514), L575 (≠ S517)
5oc1A Crystal structure of aryl-alcohol oxidase from pleurotus eryngii in complex with p-anisic acid (see paper)
30% identity, 95% coverage: 4:528/551 of query aligns to 3:561/565 of 5oc1A
- active site: V339 (≠ K318), N413 (≠ Q378), A414 (= A379), I499 (≠ A466), H501 (= H468), A544 (≠ G511), H545 (≠ N512)
- binding 4-methoxybenzoic acid: Y91 (≠ G90), I356 (≠ Q337), I390 (vs. gap), F396 (= F359), T412 (≠ H375), I499 (≠ A466), H501 (= H468), H545 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), N11 (≠ S12), A12 (= A13), E32 (= E32), A33 (≠ H33), W60 (= W59), P78 (= P77), G80 (= G79), G85 (= G84), S86 (= S85), H90 (≠ N89), Y91 (≠ G90), V93 (= V92), V230 (≠ A219), S270 (≠ A252), A271 (= A253), G272 (≠ S254), F500 (≠ Y467), H545 (≠ N512), T546 (≠ L513), Q547 (≠ N514), I550 (≠ S517)
3fimB Crystal structure of aryl-alcohol-oxidase from pleurotus eryingii (see paper)
29% identity, 95% coverage: 4:528/551 of query aligns to 3:561/565 of 3fimB
- active site: V339 (≠ K318), N413 (≠ Q378), A414 (= A379), I499 (≠ A466), H501 (= H468), A544 (≠ G511), H545 (≠ N512)
- binding flavin-adenine dinucleotide: G8 (= G9), N11 (≠ S12), A12 (= A13), E32 (= E32), A33 (≠ H33), W60 (= W59), P78 (= P77), G80 (= G79), G85 (= G84), S86 (= S85), H90 (≠ N89), Y91 (≠ G90), V93 (= V92), V230 (≠ A219), S270 (≠ A252), A271 (= A253), F500 (≠ Y467), H501 (= H468), H545 (≠ N512), T546 (≠ L513), Q547 (≠ N514), I550 (≠ S517)
4h7uA Crystal structure of pyranose dehydrogenase from agaricus meleagris, wildtype (see paper)
31% identity, 96% coverage: 1:528/551 of query aligns to 14:572/577 of 4h7uA
- active site: A343 (≠ K318), V426 (≠ G382), Y510 (≠ A466), H512 (= H468), A555 (≠ G511), H556 (≠ N512)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl (2R,3S,4S)-2,3,4-trihydroxy-5-[(4aR)-4a-hydroxy-7,8-dimethyl-2,4-dioxo-3,4,4a,5-tetrahydrobenzo[g]pteridin-10(2H)-yl]pentyl dihydrogen diphosphate (non-preferred name): G22 (= G9), G24 (= G11), T25 (≠ S12), A26 (= A13), E46 (= E32), A47 (≠ H33), W74 (= W59), G99 (= G84), C100 (≠ S85), H103 (≠ I88), N104 (= N89), G105 (= G90), V107 (= V92), L242 (= L218), V243 (≠ A219), G282 (≠ A253), G283 (≠ S254), A286 (≠ N257), H512 (= H468), A546 (≠ S502), H556 (≠ N512), T557 (≠ L513), Q558 (≠ N514), V561 (≠ S517)
Q3L245 Pyranose dehydrogenase 1; PDH 1; Pyranose:quinone oxidoreductase 1; EC 1.1.99.29 from Leucoagaricus meleagris (Western flat-topped agaric) (Agaricus meleagris) (see 2 papers)
31% identity, 96% coverage: 1:528/551 of query aligns to 39:597/602 of Q3L245
- N100 (≠ G60) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H128 (≠ I88) modified: Tele-8alpha-FAD histidine
- N344 (= N290) modified: carbohydrate, N-linked (GlcNAc...) asparagine
- H537 (= H468) active site, Proton acceptor
- H581 (≠ N512) active site
Sites not aligning to the query:
E4QP00 5-(hydroxymethyl)furfural oxidase; 5-hydroxymethylfurfural oxidase; HMFO; Thiol oxidase; EC 1.1.3.47; EC 1.8.3.- from Methylovorus sp. (strain MP688) (see paper)
32% identity, 95% coverage: 4:529/551 of query aligns to 7:528/531 of E4QP00
- V101 (≠ I88) mutation to H: Abolishes activity.
- M103 (≠ G90) mutation to A: 16-fold reduction in catalytic efficiency on vanillyl alcohol.
- V367 (≠ E373) mutation to K: 1.6-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion.; mutation to R: 1.4-fold reduction in catalytic efficiency on vanillyl alcohol. Shows significantly improved activity on the aldehyde 5-formyl-2-furancarboxylate, which results in a better 5-hydroxymethylfurfural to 2,5-furandicarboxylate conversion. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with F-466.
- W369 (≠ Q378) mutation to A: 7.5-fold reduction in catalytic efficiency on vanillyl alcohol.
- V465 (≠ A466) mutation to A: 18-fold reduction in catalytic efficiency on vanillyl alcohol.
- W466 (≠ Y467) mutation to A: 39-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate.; mutation to F: 3.4-fold reduction in catalytic efficiency on vanillyl alcohol. In contrast to wild-type, is active on secondary alcohols, such as (S)-1-phenylethanol, and is strictly enantionselective as this mutant has no activity on (R)-1-phenylethanol. Shows increased activity on the aldehyde 5-formyl-2-furancarboxylate. Displays a catalytic efficiency toward 5-formyl-2-furancarboxylate that is over 1000-fold higher than that for wild-type; when associated with R-367.
- H467 (= H468) mutation to A: Abolishes activity.
- N511 (= N512) mutation to A: 53-fold reduction in catalytic efficiency on vanillyl alcohol.
4udqA Crystal structure of 5-hydroxymethylfurfural oxidase (hmfo) in the reduced state
32% identity, 95% coverage: 4:529/551 of query aligns to 3:524/525 of 4udqA
- active site: L331 (≠ F338), F364 (= F377), W365 (≠ Q378), V461 (≠ A466), H463 (= H468), A506 (≠ G511), N507 (= N512)
- binding flavin-adenine dinucleotide: G8 (= G9), G10 (= G11), T11 (≠ S12), A12 (= A13), E32 (≠ H33), A33 (≠ G34), W64 (≠ P51), G88 (= G79), G93 (= G84), G94 (≠ S85), N98 (= N89), M99 (≠ G90), V101 (= V92), V229 (≠ A219), T261 (≠ A252), A262 (= A253), W462 (≠ Y467), H463 (= H468), A497 (≠ S502), N507 (= N512), T508 (≠ L513), N509 (= N514), T512 (≠ S517)
3q9tA Crystal structure analysis of formate oxidase (see paper)
29% identity, 95% coverage: 4:528/551 of query aligns to 8:569/577 of 3q9tA
- active site: A335 (≠ L320), D422 (vs. gap), A508 (= A466), H510 (= H468), C552 (≠ G511), R553 (≠ N512)
- binding [(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methyl(2R,3S,4S)-5-(8-formyl-7-methyl-2,4-dioxo-3,4-dihydrobenzo[g]pteridin-10(2H)-yl)-2,3,4-trihydroxypentyl dihydrogendiphosphate: G15 (= G11), T16 (≠ S12), E37 (= E32), A38 (≠ H33), W65 (= W59), T85 (vs. gap), R86 (= R78), G87 (= G79), G92 (= G84), S93 (= S85), N97 (= N89), Y98 (≠ G90), F99 (≠ M91), T100 (≠ V92), S229 (≠ A219), S265 (≠ A252), Q266 (≠ A253), E270 (≠ N257), F509 (≠ Y467), D542 (= D501), A543 (≠ S502), R553 (≠ N512), I554 (≠ L513), Q555 (≠ N514), V558 (≠ S517)
5zu2A Effect of mutation (r554a) on fad modification in aspergillus oryzae rib40formate oxidase (see paper)
29% identity, 95% coverage: 4:528/551 of query aligns to 8:569/577 of 5zu2A
- binding flavin-adenine dinucleotide: G15 (= G11), T16 (≠ S12), E37 (= E32), A38 (≠ H33), W65 (= W59), T85 (vs. gap), R86 (= R78), G87 (= G79), G92 (= G84), S93 (= S85), N97 (= N89), Y98 (≠ G90), T100 (≠ V92), S229 (≠ A219), S265 (≠ A252), Q266 (≠ A253), E270 (≠ N257), F509 (≠ Y467), D542 (= D501), A543 (≠ S502), A553 (≠ N512), I554 (≠ L513), Q555 (≠ N514), V558 (≠ S517)
6ze6A Fad-dependent oxidoreductase from chaetomium thermophilum in complex with fragment 4-nitrocatechol (see paper)
30% identity, 96% coverage: 2:528/551 of query aligns to 1:578/585 of 6ze6A
- binding 4-nitrocatechol: I75 (≠ P77), L92 (≠ V94), Q306 (≠ H294), V360 (≠ A341), Y431 (≠ G382), L433 (≠ M384), N514 (≠ E464), S516 (= S465), N517 (≠ A466), H519 (= H468), G561 (= G511), S562 (≠ N512)
- binding dihydroflavine-adenine dinucleotide: G8 (= G9), G10 (= G11), I11 (≠ S12), S12 (≠ A13), E32 (= E32), A33 (≠ H33), W56 (= W59), A77 (≠ G79), G82 (= G84), G83 (≠ S85), N87 (= N89), A88 (≠ G90), V90 (= V92), L227 (= L218), V228 (≠ A219), A265 (= A253), A518 (≠ Y467), H519 (= H468), D551 (= D501), I552 (≠ S502), S562 (≠ N512), P563 (≠ L513), M564 (≠ N514)
6ze5A Fad-dependent oxidoreductase from chaetomium thermophilum in complex with fragment 2-(1h-indol-3-yl)-n-[(1-methyl-1h-pyrrol-2-yl) methyl]ethanamine (see paper)
30% identity, 96% coverage: 2:528/551 of query aligns to 1:578/585 of 6ze5A
- binding 2-(1H-indol-3-yl)-N-[(1-methyl-1H-pyrrol-2-yl)methyl]ethanamine: I75 (≠ P77), V90 (= V92), Y431 (≠ G382), N517 (≠ A466), D576 (= D526)
- binding dihydroflavine-adenine dinucleotide: G8 (= G9), G10 (= G11), I11 (≠ S12), S12 (≠ A13), E32 (= E32), A33 (≠ H33), W56 (= W59), A77 (≠ G79), G82 (= G84), G83 (≠ S85), N87 (= N89), A88 (≠ G90), V90 (= V92), L227 (= L218), V228 (≠ A219), A265 (= A253), A518 (≠ Y467), H519 (= H468), D551 (= D501), I552 (≠ S502), S562 (≠ N512), P563 (≠ L513), M564 (≠ N514)
Sites not aligning to the query:
Query Sequence
>GFF2134 FitnessBrowser__Phaeo:GFF2134
MNADYVIVGAGSAGCAMAYRLSEAGKSVLVIEHGGTDAGPFIQMPGALSYPMNMSMYDWG
YKSQPEPHLGGRELVCPRGKVVGGSSSINGMVYVRGHAGDYNHWAESGAAGWSYADVLPY
FKRMETWDDRGHGGDPDWRGTDGPLHVTRGPRDNPLHAAFVKAGEQAGYPVSKDYNGEQQ
EGFGPMEMTVYKGQRWSAANAYLKPALKRDNCEMIRALARKVVIEDGRAVGVEVERGGKI
EVIRANAEVILAASSLNSPKLLMLSGIGPAKHLAEHGIDVVVDRPGVGQNLQDHLEFYFQ
FASKQPITLFKYWNLFGKALVGAQWLFTKTGLGASNQFESAAFIRSDKGVDYPDIQYHFL
PIAVRYDGQAAAEGHGFQAHVGPMRSDSRGEVTLASADPNDAPKILFNYMSTEKDWEDFR
KCIRLTREVFAQDAMKPFVKHEIQPGDALQSDEELNGFIREHVESAYHPCGTCKMGAVED
PMAVVDPECRVIGVEGLRVADSSIFPRITNGNLNGPSIMTGEKASDHILGRRLPSSNAEP
WFNPNWEGSQR
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory