SitesBLAST
Comparing GFF2142 FitnessBrowser__WCS417:GFF2142 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
4zz7A Crystal structure of methylmalonate-semialdehyde dehydrogenase (dddc) from oceanimonas doudoroffii (see paper)
57% identity, 96% coverage: 3:483/499 of query aligns to 2:481/489 of 4zz7A
- active site: N149 (= N150), K172 (= K173), L246 (= L247), C280 (= C281), E382 (= E384), A462 (= A464)
- binding nicotinamide-adenine-dinucleotide: T146 (= T147), P147 (= P148), F148 (= F149), N149 (= N150), K172 (= K173), E175 (≠ P176), K205 (= K206), V208 (= V209), F222 (= F223), V223 (= V224), G224 (= G225), S225 (= S226), I228 (= I229), L246 (= L247), G247 (= G248), C280 (= C281), E382 (= E384), F384 (= F386)
5tjrD X-ray crystal structure of a methylmalonate semialdehyde dehydrogenase from pseudomonas sp. Aac (see paper)
56% identity, 99% coverage: 3:495/499 of query aligns to 1:468/468 of 5tjrD
- active site: N144 (= N150), K167 (= K173), L241 (= L247), C270 (= C281), E356 (= E384), A436 (= A464)
- binding adenosine-5'-diphosphate: I140 (≠ V146), T141 (= T147), F143 (= F149), K167 (= K173), E170 (≠ P176), K200 (= K206), F217 (= F223), S220 (= S226), I223 (= I229)
4iymC Crystal structure of putative methylmalonate-semialdehyde dehydrogenase from sinorhizobium meliloti 1021 complexed with NAD, target 011934
52% identity, 98% coverage: 1:488/499 of query aligns to 3:490/491 of 4iymC
- active site: N153 (= N150), K176 (= K173), F250 (≠ L247), C284 (= C281), E386 (= E384), Q466 (≠ A464)
- binding nicotinamide-adenine-dinucleotide: I149 (≠ V146), T150 (= T147), P151 (= P148), F152 (= F149), N153 (= N150), F154 (= F151), K176 (= K173), K209 (= K206), V212 (= V209), F226 (= F223), V227 (= V224), G228 (= G225), S229 (= S226), I232 (= I229), G251 (= G248), C284 (= C281), E386 (= E384), F388 (= F386)
P42412 Malonate-semialdehyde dehydrogenase; MSA dehydrogenase; Methylmalonate-semialdehyde dehydrogenase; MMSA dehydrogenase; MMSDH; MSDH; EC 1.2.1.27 from Bacillus subtilis (strain 168) (see 3 papers)
45% identity, 96% coverage: 5:483/499 of query aligns to 7:481/487 of P42412
- C36 (≠ G33) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-160; A-287; A-351 and A-413.
- R107 (= R104) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- A150 (≠ T147) binding
- F152 (= F149) binding
- C160 (≠ M157) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-287; A-351 and A-413.
- K176 (= K173) binding
- E179 (≠ P176) binding
- R180 (≠ T177) binding
- S229 (= S226) binding
- T251 (≠ G248) binding
- R283 (= R280) mutation to L: At least 50-fold decrease of the second-order rate constant for the acylation step.
- C287 (≠ I284) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-351 and A-413.
- C351 (≠ L348) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-413.
- E382 (= E384) binding
- C413 (≠ S415) mutation to A: No effect at either the structural or enzymatic levels; when associated with A-36; A-160; A-287 and A-351.
1t90A Crystal structure of methylmalonate semialdehyde dehydrogenase from bacillus subtilis
45% identity, 96% coverage: 5:483/499 of query aligns to 5:479/484 of 1t90A
- active site: N151 (= N150), K174 (= K173), L248 (= L247), C282 (= C281), E380 (= E384), A460 (= A464)
- binding nicotinamide-adenine-dinucleotide: I147 (≠ V146), A148 (≠ T147), P149 (= P148), F150 (= F149), N151 (= N150), W159 (= W158), K174 (= K173), E177 (≠ P176), R178 (≠ T177), H207 (≠ K206), V225 (= V224), G226 (= G225), S227 (= S226), V230 (≠ I229), L248 (= L247), T249 (≠ G248), C282 (= C281), E380 (= E384), F382 (= F386)
1bpwA Betaine aldehyde dehydrogenase from cod liver (see paper)
31% identity, 95% coverage: 7:480/499 of query aligns to 22:493/503 of 1bpwA
- active site: N166 (= N150), K189 (= K173), E263 (≠ L247), C297 (= C281), E400 (= E384), E477 (≠ M462)
- binding nicotinamide-adenine-dinucleotide: I162 (≠ V146), L163 (≠ T147), W165 (≠ F149), N166 (= N150), K189 (= K173), G221 (≠ D205), G225 (≠ V209), T240 (≠ V224), G241 (= G225), S242 (= S226), T245 (≠ I229), E263 (≠ L247), L264 (≠ G248), C297 (= C281), E400 (= E384), F402 (= F386), F466 (= F451)
P56533 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABADH; Aldehyde dehydrogenase family 9 member A1; Betaine aldehyde dehydrogenase; BADH; EC 1.2.1.47; EC 1.2.1.3 from Gadus morhua subsp. callarias (Baltic cod) (Gadus callarias) (see paper)
31% identity, 95% coverage: 7:480/499 of query aligns to 22:493/503 of P56533
6vr6D Structure of aldh9a1 complexed with NAD+ in space group p1 (see paper)
32% identity, 92% coverage: 21:479/499 of query aligns to 28:482/493 of 6vr6D
- active site: N156 (= N150), E253 (≠ L247), C287 (= C281), E467 (≠ M462)
- binding nicotinamide-adenine-dinucleotide: I152 (≠ V146), G153 (≠ T147), W155 (≠ F149), K179 (= K173), A212 (≠ K206), G215 (≠ V209), Q216 (≠ D210), F229 (= F223), G231 (= G225), S232 (= S226), T235 (≠ I229), I239 (= I233)
P49189 4-trimethylaminobutyraldehyde dehydrogenase; TMABA-DH; TMABALDH; Aldehyde dehydrogenase E3 isozyme; Aldehyde dehydrogenase family 9 member A1; Formaldehyde dehydrogenase; Gamma-aminobutyraldehyde dehydrogenase; R-aminobutyraldehyde dehydrogenase; EC 1.2.1.47; EC 1.2.1.3; EC 1.2.1.46; EC 1.2.1.19 from Homo sapiens (Human) (see 2 papers)
32% identity, 92% coverage: 21:479/499 of query aligns to 29:483/494 of P49189
- C116 (≠ I108) to S: in allele ALDH9A1*2
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed; alternate
- 2 modified: N-acetylserine; in 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed
8skfA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (lattice translocation disorder)
35% identity, 92% coverage: 22:479/499 of query aligns to 32:486/497 of 8skfA
- binding calcium ion: T33 (≠ V23), I34 (≠ F24), D100 (≠ E90), V187 (≠ T177)
- binding nicotinamide-adenine-dinucleotide: I156 (≠ V146), G157 (≠ T147), A158 (≠ P148), W159 (≠ F149), K183 (= K173), E186 (≠ P176), G216 (≠ D205), G220 (≠ V209), T235 (≠ V224), G236 (= G225), G237 (≠ S226), S240 (≠ I229), K243 (≠ Y232), E259 (≠ L247), C293 (= C281), F396 (= F386)
8vr1A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (ctp bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8vr1A
8vr0A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (gmp bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8vr0A
8vqzA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (cmp bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8vqzA
8vqwC Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (coa bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8vqwC
- binding coenzyme a: I147 (≠ V146), W150 (≠ F149), K174 (= K173), S176 (= S175), E177 (≠ P176), G207 (≠ D205), G211 (≠ V209), F225 (= F223), G227 (= G225), G228 (≠ S226), S231 (≠ I229), H331 (≠ A328), F387 (= F386)
8vj3A Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (fad bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8vj3A
8uzoA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (adp bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8uzoA
8uznA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (amp bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8uznA
- binding adenosine monophosphate: I147 (≠ V146), G148 (≠ T147), K174 (= K173), S176 (= S175), E177 (≠ P176), G207 (≠ D205), G211 (≠ V209), F225 (= F223), G228 (≠ S226), S231 (≠ I229), K234 (≠ Y232)
8uzmA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADPH bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8uzmA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G148 (≠ T147), W150 (≠ F149), K174 (= K173), S176 (= S175), E177 (≠ P176), G207 (≠ D205), G211 (≠ V209), F225 (= F223), T226 (≠ V224), G227 (= G225), G228 (≠ S226), S231 (≠ I229), E250 (≠ L247), G252 (= G249), C284 (= C281), E385 (= E384), F387 (= F386)
8uzkA Crystal structure of betaine aldehyde dehydrogenase (betb) from klebsiella aerogenes (NADP+ bound)
35% identity, 92% coverage: 22:479/499 of query aligns to 23:477/488 of 8uzkA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I147 (≠ V146), G148 (≠ T147), W150 (≠ F149), N151 (= N150), K174 (= K173), S176 (= S175), E177 (≠ P176), G207 (≠ D205), G211 (≠ V209), F225 (= F223), G227 (= G225), G228 (≠ S226), S231 (≠ I229), E250 (≠ L247), F387 (= F386)
4cazA Crystal structure of betaine aldehyde dehydrogenase from pseudomonas aeruginosa in complex with nadh
33% identity, 92% coverage: 22:479/499 of query aligns to 24:478/489 of 4cazA
- active site: N152 (= N150), K175 (= K173), E251 (≠ L247), C285 (= C281), E386 (= E384), E463 (≠ M462)
- binding [[(2R,3S,4R,5R)-5-[(3R)-3-aminocarbonyl-3,4-dihydro-2H-pyridin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanidyl-phosphoryl] [(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl phosphate: I148 (≠ V146), G149 (≠ T147), W151 (≠ F149), N152 (= N150), K175 (= K173), E178 (≠ P176), G208 (≠ K206), G212 (≠ D210), F226 (= F223), T227 (≠ V224), G228 (= G225), G229 (≠ S226), T232 (≠ I229), V236 (≠ I233), E251 (≠ L247), L252 (≠ G248), C285 (= C281), E386 (= E384), F388 (= F386)
Query Sequence
>GFF2142 FitnessBrowser__WCS417:GFF2142
MTTTIEHYINDQRVSRDDRYQDVFNPATGEKTGRVALASRQTVSEAVAAAQAAFAGWADT
PPIRRARVLFEYLHLLRERKDDLARIIVAEHGKVFTDAQGEVDRGIDILEFACGIPNLLK
GEHSDQVSRGMDNWTMRQPLGVVAGVTPFNFPVMVPMWMYPIAIAAGNTFILKPSPTDPS
ASLFMAELLREAGLPKGVFNVVQGDKESVDALIEHPDVKAVSFVGSTPIAQYIYETGARN
GKRVQGLGGAKNHMVVMPDADIEKTVDALMGAAYGSAGERCMAISVAVLVGDVGDKVIAA
LTERAKHLRITDGRDLKAEMGPIVSRAALERISGYIEQGVQAGAQLLLDGRDYVPTEPGL
ENGFWLGATLFDHVTEEMSIYREEIFGPVLACVRVNDFAEAIKLVNAHEFGNGVSCFTRD
GNIAREFARRIEVGMVGINVPISVPMAWHGFGGWKKSLFGDMHAYGTEGVRFYTKQKSIM
QRWSESIEQGAEFAMPVSK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory