SitesBLAST
Comparing GFF2149 FitnessBrowser__WCS417:GFF2149 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
25% identity, 77% coverage: 166:784/802 of query aligns to 39:543/690 of Q8GR64
- E81 (= E213) binding
- C127 (= C260) modified: Disulfide link with 128
- C128 (vs. gap) modified: Disulfide link with 127
- R133 (= R261) binding
- T177 (≠ S344) binding
- GA 193:194 (≠ VA 360:361) binding
- E195 (≠ D362) binding
- T252 (≠ N410) binding
- N272 (≠ S430) binding
- D317 (= D474) binding
- K344 (= K503) binding
- NW 404:405 (= NW 602:603) binding
Sites not aligning to the query:
- 1:22 signal peptide
- 23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
- 547 binding
- 613 binding covalent
- 616 binding covalent
- 617 binding axial binding residue
- 655 binding axial binding residue
1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
25% identity, 77% coverage: 166:784/802 of query aligns to 17:521/664 of 1kv9A
- active site: E173 (≠ D362), N250 (≠ S430), D295 (= D474)
- binding acetone: E173 (≠ D362), D295 (= D474)
- binding calcium ion: E173 (≠ D362), N250 (≠ S430), D295 (= D474)
- binding heme c: A101 (≠ V256), R102 (≠ W257)
- binding pyrroloquinoline quinone: E59 (= E213), C105 (= C260), C106 (vs. gap), R111 (= R261), T155 (≠ S344), G170 (≠ R359), A172 (= A361), E173 (≠ D362), T230 (≠ N410), W232 (= W412), K322 (= K503), N382 (= N602), W383 (= W603), W460 (≠ E701)
Sites not aligning to the query:
- binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636
- binding pyrroloquinoline quinone: 525
Q46444 Quinohemoprotein alcohol dehydrogenase; QH-ADH; Alcohol dehydrogenase (azurin); PQQ-containing alcohol dehydrogenase; PQQ-dependent ADH; Quinohaemoprotein ethanol dehydrogenase type I; QH-EDHI; EC 1.1.9.1 from Comamonas testosteroni (Pseudomonas testosteroni) (see 3 papers)
25% identity, 77% coverage: 166:784/802 of query aligns to 59:571/708 of Q46444
- E101 (= E213) binding
- C147 (= C260) modified: Disulfide link with 148
- C148 (vs. gap) modified: Disulfide link with 147
- R153 (= R261) binding
- T198 (≠ S344) binding
- GA 214:215 (≠ VA 360:361) binding
- E216 (≠ D362) binding
- T274 (≠ N410) binding
- N294 (≠ S430) binding
- D339 (= D474) binding
- K366 (= K503) binding
- NW 425:426 (= NW 602:603) binding
Sites not aligning to the query:
- 1:31 signal peptide
- 575 binding
- 635 binding covalent
- 638 binding covalent
- 639 binding axial binding residue
- 678 binding axial binding residue
1kb0A Crystal structure of quinohemoprotein alcohol dehydrogenase from comamonas testosteroni (see paper)
25% identity, 77% coverage: 166:784/802 of query aligns to 28:540/670 of 1kb0A
- active site: E185 (≠ D362), N263 (≠ S430), D308 (= D474)
- binding calcium ion: E185 (≠ D362), N263 (≠ S430), D308 (= D474)
- binding pyrroloquinoline quinone: E70 (= E213), C116 (= C260), C117 (vs. gap), R122 (= R261), T167 (≠ S344), G182 (≠ R359), G183 (≠ V360), A184 (= A361), E185 (≠ D362), T243 (≠ N410), W245 (= W412), D308 (= D474), K335 (= K503), N394 (= N602), W395 (= W603), W479 (≠ L706)
- binding tetrahydrofuran-2-carboxylic acid: C116 (= C260), C117 (vs. gap), E185 (≠ D362), D308 (= D474), P389 (≠ S597)
Sites not aligning to the query:
- binding heme c: 598, 599, 602, 603, 617, 620, 631, 637, 640, 642, 643, 645
- binding pyrroloquinoline quinone: 543, 544
7o6zB Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
24% identity, 77% coverage: 165:784/802 of query aligns to 12:541/588 of 7o6zB
- binding methanol: E173 (≠ M368), W263 (vs. gap), D314 (= D474)
- binding Neodymium Ion: E173 (≠ M368), N259 (≠ S430), D314 (= D474), D316 (= D476)
- binding pyrroloquinoline quinone: E55 (= E213), C105 (≠ I283), C106 (≠ M284), R111 (≠ P289), T155 (≠ V336), G170 (≠ Q365), G171 (≠ T366), D172 (= D367), E173 (≠ M368), W241 (= W412), D316 (= D476), R341 (≠ K503), D403 (≠ N602), W481 (≠ L721)
Sites not aligning to the query:
7o6zA Structure of a neodymium-containing, xoxf1-type methanol dehydrogenase (see paper)
24% identity, 77% coverage: 165:784/802 of query aligns to 12:541/588 of 7o6zA
Sites not aligning to the query:
O05542 Alcohol dehydrogenase (quinone), dehydrogenase subunit; ADH; Alcohol dehydrogenase (quinone), acceptor subunit; Alcohol dehydrogenase (quinone), subunit I; Ethanol:Q2 reductase; G3-ADH subunit I; Quinohemoprotein alcohol dehydrogenase; Quinohemoprotein-cytochrome c complex; Ubiquinol oxidase; EC 1.1.5.5 from Gluconobacter oxydans (strain 621H) (Gluconobacter suboxydans) (see paper)
24% identity, 80% coverage: 145:784/802 of query aligns to 32:584/757 of O05542
- Q35 (≠ P148) modified: Pyrrolidone carboxylic acid
Sites not aligning to the query:
8gy2A Cryo-em structure of membrane-bound alcohol dehydrogenase from gluconobacter oxydans
24% identity, 79% coverage: 152:784/802 of query aligns to 5:550/723 of 8gy2A
- binding calcium ion: E181 (≠ D362), N263 (≠ S430), D308 (= D474)
- binding heme c: D104 (≠ Q258)
- binding pyrroloquinoline quinone: C107 (vs. gap), C108 (vs. gap), D163 (≠ S344), G179 (≠ V360), A180 (= A361), E181 (≠ D362), W245 (= W412), N263 (≠ S430), D308 (= D474), K335 (= K503), F398 (≠ W603), W489 (≠ L706)
Sites not aligning to the query:
- binding heme c: 618, 619, 622, 623, 633, 634, 636, 639, 652, 660, 662, 665
Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
24% identity, 78% coverage: 158:784/802 of query aligns to 42:567/718 of Q4W6G0
- C138 (≠ Y265) modified: Disulfide link with 139
- C139 (≠ F266) modified: Disulfide link with 138
- R144 (≠ A290) binding
- T189 (≠ S344) binding
- GA 205:206 (≠ VA 360:361) binding
- E207 (≠ D362) binding
- T264 (≠ N410) binding
- N284 (≠ S430) binding
- D329 (= D474) binding
- K356 (= K503) binding
- W415 (≠ L598) binding
- DW 419:420 (≠ NW 602:603) binding
Sites not aligning to the query:
- 1:29 signal peptide
- 30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
- 635 binding covalent
- 638 binding covalent
- 639 binding axial binding residue
- 676 binding axial binding residue
1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
24% identity, 78% coverage: 158:784/802 of query aligns to 13:538/684 of 1yiqA
- active site: E178 (≠ D362), N255 (≠ S430), D300 (= D474)
- binding calcium ion: E178 (≠ D362), N255 (≠ S430), D300 (= D474)
- binding pyrroloquinoline quinone: E63 (= E213), C109 (≠ Y265), C110 (≠ F266), R115 (≠ A290), T160 (≠ S344), G175 (≠ R359), G176 (≠ V360), A177 (= A361), E178 (≠ D362), T235 (≠ N410), W237 (= W412), K327 (= K503), D390 (≠ N602), W391 (= W603), F477 (≠ E701)
Sites not aligning to the query:
- binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
- binding pyrroloquinoline quinone: 542
6damA Crystal structure of lanthanide-dependent methanol dehydrogenase xoxf from methylomicrobium buryatense 5g (see paper)
24% identity, 79% coverage: 166:802/802 of query aligns to 13:562/563 of 6damA
- active site: E171 (≠ V372), N259 (≠ S430), D301 (= D474)
- binding pyrroloquinoline quinone: E55 (≠ D208), C103 (= C260), C104 (vs. gap), R109 (= R261), T153 (≠ S344), S168 (≠ P369), G169 (= G370), G170 (= G371), E171 (≠ V372), T239 (≠ N410), W241 (= W412), D303 (= D476), R328 (≠ K503), N394 (= N602), W480 (≠ L721), G543 (≠ R787), W544 (≠ Q788)
6fkwA Europium-containing methanol dehydrogenase (see paper)
24% identity, 77% coverage: 167:784/802 of query aligns to 14:528/576 of 6fkwA
- active site: E172 (≠ V372), N256 (≠ S432), D299 (= D474), D301 (= D476)
- binding europium ion: E172 (≠ V372), N256 (≠ S432), D299 (= D474), D301 (= D476)
- binding pyrroloquinoline quinone: E55 (≠ D208), C104 (= C260), C105 (vs. gap), R110 (= R261), T154 (≠ S344), S169 (≠ P369), G170 (= G370), G171 (= G371), E172 (≠ V372), T236 (≠ N410), W238 (= W412), D301 (= D476), R326 (≠ K503), D388 (≠ N602), W467 (≠ L721)
Sites not aligning to the query:
4maeA Methanol dehydrogenase from methylacidiphilum fumariolicum solv (see paper)
24% identity, 77% coverage: 167:784/802 of query aligns to 14:528/577 of 4maeA
- active site: E172 (≠ V372), N256 (≠ S432), D299 (= D474)
- binding cerium (iii) ion: E172 (≠ V372), N256 (≠ S432), D299 (= D474), D301 (= D476)
- binding pyrroloquinoline quinone: E55 (≠ D208), C104 (= C260), C105 (vs. gap), R110 (= R261), T154 (≠ S344), S169 (≠ P369), G170 (= G370), G171 (= G371), E172 (≠ V372), T236 (≠ N410), W238 (= W412), D301 (= D476), R326 (≠ K503), D388 (≠ N602), W467 (≠ L721)
Sites not aligning to the query:
6zcvA Crystal structure of lanthanide-dependent alcohol dehydrogenase pedh from pseudomonas putida kt2440
23% identity, 76% coverage: 176:784/802 of query aligns to 26:526/562 of 6zcvA
- active site: E172 (≠ R359), N254 (≠ S430), D296 (= D474)
- binding calcium ion: N161 (≠ G351), K163 (vs. gap), P278 (≠ A456), D279 (≠ S457)
- binding pyrroloquinoline quinone: Q60 (= Q215), C104 (≠ I283), C105 (≠ M284), I108 (≠ S287), R110 (≠ P289), S154 (= S344), G170 (= G357), G171 (= G358), E172 (≠ R359), W236 (= W412), D298 (= D476), R323 (≠ K503), N390 (= N602), W466 (≠ L721)
Sites not aligning to the query:
6oc6A Lanthanide-dependent methanol dehydrogenase xoxf from methylobacterium extorquens, in complex with lanthanum and pyrroloquinoline quinone (see paper)
26% identity, 49% coverage: 170:558/802 of query aligns to 21:377/579 of 6oc6A
- active site: E171 (≠ V372), N255 (≠ S430), D297 (= D474)
- binding pyrroloquinoline quinone: E55 (≠ D208), C103 (= C260), C104 (vs. gap), R109 (= R261), T153 (≠ S345), S168 (≠ P369), G169 (= G370), G170 (= G371), E171 (≠ V372), W237 (= W412), D299 (= D476), R324 (≠ K503)
Sites not aligning to the query:
P12293 Methanol dehydrogenase [cytochrome c] subunit 1; MDH large subunit alpha; MEDH; EC 1.1.2.7 from Paracoccus denitrificans (see 2 papers)
25% identity, 50% coverage: 118:515/802 of query aligns to 8:375/631 of P12293
- C135 (≠ R259) modified: Disulfide link with 136
- C136 (= C260) modified: Disulfide link with 135
Sites not aligning to the query:
- 1:32 signal peptide
- 418 modified: Disulfide link with 447
- 447 modified: Disulfide link with 418
1lrwA Crystal structure of methanol dehydrogenase from p. Denitrificans (see paper)
24% identity, 44% coverage: 166:515/802 of query aligns to 13:343/600 of 1lrwA
- active site: E177 (≠ D362), N261 (≠ S432), D303 (= D474)
- binding calcium ion: E177 (≠ D362), N261 (≠ S432), D303 (= D474)
- binding pyrroloquinoline quinone: E55 (≠ D208), C103 (≠ R259), C104 (= C260), R109 (= R261), T159 (≠ S344), S174 (≠ R359), G175 (≠ V360), A176 (= A361), E177 (≠ D362), T241 (≠ N410), W243 (= W412), R331 (≠ K503)
Sites not aligning to the query:
7ce5A Methanol-pqq bound methanol dehydrogenase (mdh) from methylococcus capsulatus (bath) (see paper)
24% identity, 44% coverage: 165:515/802 of query aligns to 12:342/573 of 7ce5A
- active site: E177 (≠ D362), N261 (≠ S432), D303 (= D474)
- binding calcium ion: E177 (≠ D362), N261 (≠ S432), D303 (= D474)
- binding methanol: E177 (≠ D362)
- binding pyrroloquinoline quinone: E55 (≠ D208), C103 (≠ R259), C104 (= C260), R109 (= R261), T159 (≠ S344), A174 (≠ R359), G175 (≠ V360), A176 (= A361), E177 (≠ D362), T241 (≠ N410), W243 (= W412), R330 (≠ K503)
Sites not aligning to the query:
7cdlC Holo-methanol dehydrogenase (mdh) with cys131-cys132 reduced from methylococcus capsulatus (bath) (see paper)
24% identity, 44% coverage: 165:515/802 of query aligns to 12:342/573 of 7cdlC
- active site: E177 (≠ D362), N261 (≠ S432), D303 (= D474)
- binding calcium ion: E177 (≠ D362), N261 (≠ S432), D303 (= D474)
- binding pyrroloquinoline quinone: E55 (≠ D208), R109 (= R261), T159 (≠ S344), A174 (≠ R359), A176 (= A361), E177 (≠ D362), T241 (≠ N410), W243 (= W412), D303 (= D474), R330 (≠ K503)
Sites not aligning to the query:
4mh1B Crystal structure and functional studies of quinoprotein l-sorbose dehydrogenase from ketogulonicigenium vulgare y25 (see paper)
21% identity, 79% coverage: 166:802/802 of query aligns to 19:513/514 of 4mh1B
- active site: D240 (= D474)
- binding pyrroloquinoline quinone: Q60 (= Q215), F106 (≠ V256), R111 (= R261), S154 (= S344), T169 (≠ P369), W196 (= W412), E242 (≠ D476), K284 (= K542), W346 (= W603), Y421 (≠ H711), Y485 (vs. gap)
Query Sequence
>GFF2149 FitnessBrowser__WCS417:GFF2149
MKRASRAAGVSRLLLLGLGVIIALLGLALAIGGVKLVSLGGSWYFLIGGAIMAIAGLLIA
CRKPAGAWVFAAFLIGTAIWAVADVGLVFWPLFSRLFMFAAIGMVVALVYPLLVNKPARG
AYAVAAVLAAGVAVAAGNMFVAHPSVAPTGAGPGITPVAAGDAQKDWAHYGNTEGGSRFA
ALDQINRDTVNKLKVAWTYHTGDVAISDGNGAEDQLTPLQIGNKVFICTPHNNLIALDAD
TGKELWKNEVNAKSAVWQRCRGMAYFDATAPIAQPTQPNSSPIMPASVPAGAQCQRRLLT
NTIDARLIAVDADTGKFCEDFGTHGQVDLKAGLGNVPDSYYQLSSAPLIAGTTVVVGGRV
ADNVQTDMPGGVIRGFDVISGQMRWAFDPGNPEDKQAPADGSTYVRSTPNSWAPMSYDPL
MNTVFLPMGSSSTDIYGVERTQLNHKYGASVLALDASTGAEKWVYQTVHNDLWDFDLPMQ
PSLIDFTPPGSDKAVPAVVIGTKAGQIYVLDRATGKPLTDVQEVPVKAANIPNEPYSPTQ
PKSVGMPQIGAQTLTESDMWGATPFDQLLCRIDFKGMRYEGLYTAPGTDKSLSFPGSLGG
MNWGSISTDPVHGFIFVNDMRLGLWIQMVPSQNKAQASSGGEALNTGMGAVPLKGTPYAV
NKNRFLSVAGIPCQAPPFGTLTAIDMKTQKVAWQVPVGTVEDTGPLGIRMHLPIKIGLPT
LGGTLSTQGGLIFIAGTQDFYLRAFNSGNGDEVWKARLPVGSQGGPMTYVSPKTGKQYIV
ITAGGARQSTDRGDYVIAYALP
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory