SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing GFF2178 FitnessBrowser__psRCH2:GFF2178 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P0A717 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Escherichia coli (strain K12) (see 4 papers)
32% identity, 96% coverage: 8:312/317 of query aligns to 6:300/315 of P0A717

query
sites
P0A717

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D129 (≠ E134) to A: in mutant PRSA1; alters the binding of divalent cations, especially magnesium. Little alteration in the affinity for ribose 5-phosphate and 27-fold decrease of the affinity for ATP. Absence of inhibition by AMP
D220 (= D231) mutation to E: 4-fold decrease in the affinity binding for Rib-5-P in the presence of magnesium ions. In the presence of cobalt ions, it shows a 15-fold decrease in the affinity binding for Rib-5-P.; mutation to F: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.
D221 (= D232) mutation to A: The affinity binding for ATP is comparable to those of the wild-type, apart from a slight decrease in the presence of manganese ions. The affinity binding for Rib-5-P is greatly decreased in the presence of both manganese and cobalt ions but only about 2-fold in the presence of magnesium ions.
D224 (≠ S235) mutation to A: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.; mutation to S: With magnesium or manganese ions, the affinity binding values for ATP and Rib-5-P are comparable to those of the wild-type.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4s2uA Crystal structure of the phosphorybosylpyrophosphate synthetase from e. Coli
32% identity, 96% coverage: 8:312/317 of query aligns to 5:299/308 of 4s2uA

query
sites
4s2uA

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binding magnesium ion: D223 (≠ S235), T227 (= T239)

6asvC E. Coli prpp synthetase (see paper)
32% identity, 96% coverage: 8:312/317 of query aligns to 4:298/311 of 6asvC

query
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6asvC

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binding phosphate ion: D168 (= D177), D218 (= D231), D219 (= D232), D222 (≠ S235), T223 (= T236), G224 (= G237), T226 (= T239), L227 (= L240)

8dbkB Human prps1 with phosphate, atp, and r5p; hexamer with resolved catalytic loops (see paper)
30% identity, 96% coverage: 8:312/317 of query aligns to 5:299/316 of 8dbkB

query
sites
8dbkB

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V

L

I

D

D

S

I

S

S

A

M

M

I

L

L

A

A

A

E

A

A

L

I

binding adenosine monophosphate: R95 (= R102), Q96 (≠ K103), N199 (= N210)
binding adenosine-5'-triphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41)
binding phosphate ion: S46 (≠ P49), R48 (≠ E51)
binding 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose: H129 (= H136), D170 (= D177), G172 (= G179), K193 (≠ M204), R195 (≠ K206), D219 (= D231), D220 (= D232), D223 (≠ S235), T224 (= T236), C225 (≠ G237), G226 (= G238), T227 (= T239)

8dbeA Human prps1 with adp; hexamer (see paper)
30% identity, 96% coverage: 8:312/317 of query aligns to 5:299/316 of 8dbeA

query
sites
8dbeA

L

I

F

F

A

S

L

G

Q

S

G

S

T

H

Q

Q

E

D

Y

L

A

S

A

Q

R

K

V

I

A

A

R

D

H

R

M

L

G

G

L

L

E

E

L

L

A

G

E

K

I

V

E

V

E

T

R

K

A

K

F
|
F

E

S

D
x
N

G

Q

E
|
E

H

T

K

C

S

V

R

E

A

I

L

G

T

E

P

S

V

V

E

R

G

G

R

E

K

D

V

V

A

Y

V

I

F

V

H

Q

A

S

L

G

Y

C

G

G

D

E

D

-

E

-

R

-

S

-

V

I

N

N

D

D

K

N

L

L

C

M

R

E

L

L

F

I

F

M

C

I

G

N

A

A

L

C

K

K

D

I

A

A

G

S

A

A

R

S

H

R

V

V

Q

T

V

A

V

V

A

I

P

P

Y

C

L

F

C

P

Y

Y

A

A

R
|
R

K
x
Q

E

D

R
x
K

T
x
D

Q

K

F
x
S

Q
x
R

D

A

P

P

I

I

I

S

T

A

R

K

Y

L

V

V

A

A

A

N

L

M

F

L

E

S

A

V

C

A

R

G

V

A

D

D

R

H

L

I

T

I

T

T

L

M

E

D

V

L

H
|
H

N

-

L

A

A

S

A

Q

F

I

D

Q

N

G

A

F

F

F

R

D

I

I

P

P

T

V

R
x
D

H

N

I

L

E
x
Y

S

A

A

E

E

P

L

A

F

V

A

L

A

K

H

W

F

I

A

R

Q

E

-

N

L

I

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S

A

E

D

W

A

R

E

N

L

C

V

T

A

I

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S

P

P

D
|
D

A

A

G

G

A

A

K

K

R

R

A

V

E

T

S

S

F

I

R

A

R

D

A

R

L

L

Q

N

Q

V

H

-

T

-

G

-

R

-

S

D

V

F

G

A

S

L

A

I

Y

H

M

K

E

E

K

R

Y

K

R

K

S

A

N

N

D

E

I

-

V

V

T

D

G

R

T

M

M

V

L

L

V

V

G

G

D

D

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V

R

K

D

D

R

R

I

V

A

A

I

I

I

L

V

V

D
|
D

L

M

I

A

S
x
D

T
|
T

G

C

G
|
G

T
|
T

L

I

L

C

R

H

A

A

G

A

E

D

A

K

C

L

R

L

K

S

A

A

G

G

A

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Q

R

V

V

H

Y

A

A

A

I

A

L

A

T

H

H

G

G

L

I

F

F

T

S

G

-

G

G

P

P

E

A

L

I

-

S

-

R

L

I

E

N

S

N

P

A

V

C

F

F

D

E

Q

A

L

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V

V

I

V

T

T

D

N

T

T

V

I

P

P

P

-

F

-

R

Q

L

E

D

D

P

K

D

M

L

K

A

H

G

C

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K

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V

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D

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I

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L

A

A

A

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A

A

L

I

binding adenosine-5'-diphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), R95 (= R102), Q96 (≠ K103), K98 (≠ R105), K99 (≠ R106), D100 (≠ T107), S102 (≠ F109), R103 (≠ Q110), H129 (= H136), D142 (≠ R150), Y145 (≠ E153)
binding 5-O-phosphono-alpha-D-ribofuranose: H129 (= H136), D170 (= D177), D219 (= D231), D220 (= D232), D223 (≠ S235), T224 (= T236), G226 (= G238), T227 (= T239)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 307, 308, 309, 312

7yk1A Structural basis of human prps2 filaments (see paper)
30% identity, 97% coverage: 7:312/317 of query aligns to 4:290/306 of 7yk1A

query
sites
7yk1A

L

V

L

L

F

F

A

S

L

G

Q

S

G

S

T

H

Q

Q

E

D

Y

L

A

S

A

Q

R

R

V

V

A

A

R

D

H

R

M

L

G

G

L

L

E

E

L

L

A

G

E

K

I

V

E

V

E

T

R

K

A

K

F
|
F

E

S

D
x
N

G

Q

E
|
E

H

T

K

S

S

V

R

E

A

I

L

G

T

E

P
x
S

V

V

E
x
R

G

G

R

E

K

D

V

V

A

Y

V

I

F

I

H

Q

A

S

L

G

Y

C

G

G

D

E

D

-

E

-

R

-

S

-

V

I

N

N

D

D

K

N

L

L

C

M

R

E

L

L

F

I

F

M

C

I

G

N

A

A

L

C

K

K

D

I

A

A

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A

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S

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R

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T

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A

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Y

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L

F

C

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Y

Y

A

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R
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R

K

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K

R
x
K

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x
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K

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x
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x
R

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A

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P

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Y

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V

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A

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H

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L

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A

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x
D

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N

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A

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L

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A

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-

N

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P

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D

A

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G

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A

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F

I

R

A

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D

A

R

L

L

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-

H

-

T

-

G

-

R

-

S

N

V

V

G

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S

F

A

A

Y

L

M

I

E

H

K

K

Y

E

R

-

S

-

N

-

D

-

I

-

V

-

T

-

G

-

T

M

M

V

L

L

V

V

G

G

D

D

V

V

R

K

D

D

R

R

I

V

A

A

I

I

I

L

V

V

D

D

L

M

I

A

S
x
D

T

T

G
x
C

G

G

T
|
T

L

I

L

C

R

H

A

A

G

A

E

D

A

K

C

L

R

L

K

S

A

A

G

G

A

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T

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K

V

V

H

Y

A

A

A

I

A

L

A

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H

H

G

G

L

I

F

F

T

S

G

-

G

G

P

P

E

A

L

I

-

S

-

R

L

I

E

N

S

N

P

A

V

A

F

F

D

E

Q

A

L

V

V

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I

V

T

T

D

N

T

T

V

I

P

P

P

-

F

-

R

Q

L

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D

D

P

K

D

M

L

K

A

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T

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K

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T

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V

V

L

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S

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M

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L

L

A

A

A

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A

A

L

I

binding adenosine-5'-diphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), S46 (≠ P49), R48 (≠ E51), R95 (= R102), K99 (≠ R106), D100 (≠ T107), K101 (≠ Q108), S102 (≠ F109), R103 (≠ Q110), H129 (= H136), D142 (≠ R150)
binding phosphate ion: D214 (≠ S235), C216 (≠ G237), T218 (= T239)

Sites not aligning to the query:

binding adenosine-5'-diphosphate: 298, 300, 303

P60891 Ribose-phosphate pyrophosphokinase 1; PPRibP; Phosphoribosyl pyrophosphate synthase I; PRS-I; EC 2.7.6.1 from Homo sapiens (Human) (see 5 papers)
30% identity, 96% coverage: 8:312/317 of query aligns to 6:300/318 of P60891

query
sites
P60891

L

I

F

F

A

S

L

G

Q

S

G

S

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H

Q

Q

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Y

L

A
x
S

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V

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A

A

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M

L

G

G

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A

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G

G

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x
D

V

V

A

Y

V

I

F

V

H

Q

A

S

L

G

Y

C

G

G

D

E

D

-

E

-

R

-

S

-

V

I

N

N

D

D

K

N

L

L

C

M

R

E

L

L

F

I

F

M

C

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G

N

A

A

L

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K

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A

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A

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R

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V

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P

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Y

A

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K

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K

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A

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P

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x
N

L

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A

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D

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L

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x
L

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H

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L

A

A
x
S

A

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Q

N

G

A

F

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I

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x
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x
N

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x
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A

A

E

E

P

L

A

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V

A

L

A

K

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W

F

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-

N

L

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A

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A

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x
D

A

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L

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x
A

S

L

A

I

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x
H

M

K

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E

K

R

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K

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K

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A

N

N

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x
D

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M

M

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L

L

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G

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D

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V

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K

D

D

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R

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A

A

I

I

I

L

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|
V

D

D

L

M

I

A

S

D

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T

G

C

G

G

T

T

L

I

L

C

R
x
H

A

A

G

A

E

D

A

K

C

L

R

L

K

S

A

A

G

G

A

A

R

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R

V

V

H

Y

A

A

A

I

A

L

A

T

H

H

G

G

L

I

F

F

T

S

G

-

G

G

P

P

E

A

L

I

-

S

-

R

L

I

E

N

S

N

P

A

V

C

F

F

D

E

Q

A

L

V

V

V

I

V

T

T

D

N

T

T

V

I

P

P

P

-

F

-

R

Q

L

E

D

D

P

K

D

M

L

K

A

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G

C

R

S

R

K

L

I

T

Q

V

V

L

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D

S

I

S

S

A

M

M

I

L

L

A

A

A

E

A

A

L

I

S16 (≠ A18) to P: found in patients with phosphoribosyl pyrophosphate synthetase I deficiency; likely pathogenic; dbSNP:rs869025594
D52 (≠ K54) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852542
N114 (≠ A120) to S: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852540
L129 (≠ V135) to I: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852543
S132 (≠ A139) mutation to A: Reduces catalytic activity.; mutation to F: No effect on catalytic activity.
V142 (≠ T149) to L: found in a patient with an intermediate phenotype between ARTS and PRPS1 superactivity; likely pathogenic; normal PRPP synthetase activity in fibroblasts; loss of activity in erythrocytes; dbSNP:rs398122855
N144 (≠ H151) mutation to H: No effect on catalytic activity.
Y146 (≠ E153) mutation to F: No effect on catalytic activity.; mutation to M: Reduces catalytic activity.
D183 (≠ R189) to H: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852541
A190 (≠ G200) to V: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852544
H193 (≠ Y203) to Q: in PRPS1 superactivity; no effect on Km; resistant to inhibition by ADP and GDP; dbSNP:rs137852545
D203 (≠ T214) to H: in a breast cancer sample; somatic mutation
V219 (= V230) to G: in a breast cancer sample; somatic mutation
H231 (≠ R242) to D: in a colorectal cancer sample; somatic mutation

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2hcrA Crystal structure of human phosphoribosyl pyrophosphate synthetase 1 in complex with amp(atp), cadmium and sulfate ion (see paper)
30% identity, 96% coverage: 8:312/317 of query aligns to 4:292/305 of 2hcrA

query
sites
2hcrA

L

I

F

F

A

S

L

G

Q

S

G

S

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H

Q

Q

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D

Y

L

A

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A

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G

L

L

E

E

L

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A

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A

K

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F

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E

A

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D

V

V

A

Y

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L

G

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C

G

G

D

E

D

-

E

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-

S

-

V

I

N

N

D

D

K

N

L

L

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M

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E

L

L

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F

M

C

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G

N

A

A

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K

D

I

A

A

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A

A

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S

H

R

V

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T

V

A

V

V

A

I

P

P

Y

C

L

F

C

P

Y

Y

A

A

R
|
R

K
x
Q

E

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R
x
K

R

K

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K

F

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Q

R

D

A

P

P

I

I

I

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V

A

A

A

N

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A

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A

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D

R

H

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M

E

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V

L

H
|
H

N

-

L

A

A

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A

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N

G

A

F

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A

A

E

E

P

L

A

F

V

A

L

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K

H

W

F

I

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E

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N

L

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S

A

E

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W

A

R

E

N

L

C

V

T

A

I

V

V

S

S

P

P

D

D

A

A

G

G

A

A

K

K

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R

A

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S

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A

R

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A

R

L

L

Q

-

H

-

T

-

G

-

R

-

S

N

V

V

G

D

S

F

A

A

Y

L

M

I

E

H

K

K

Y

E

R

R

S

D

N

R

D

-

I

-

V

-

T

-

G

-

T

M

M

V

L

L

V

V

G

G

D

D

V

V

R

K

D

D

R

R

I

V

A

A

I

I

I

L

V

V

D

D

L

M

I

A

S

D

T

T

G

C

G

G

T

T

L

I

L

C

R

H

A

A

G

A

E

D

A

K

C

L

R

L

K

S

A

A

G

G

A

A

R

T

Q

R

V

V

H

Y

A

A

A

I

A

L

A

T

H

H

G

G

L

I

F

F

T

S

G

-

G

G

P

P

E

A

L

I

-

S

-

R

L

I

E

N

S

N

P

A

V

C

F

F

D

E

Q

A

L

V

V

V

I

V

T

T

D

N

T

T

V

I

P

P

P

-

F

-

R

Q

L

E

D

D

P

K

D

M

L

K

A

H

G

C

R

S

R

K

L

I

T

Q

V

V

L

I

D

D

S

I

S

S

A

M

M

I

L

L

A

A

A

E

A

A

L

I

binding adenosine monophosphate: R94 (= R102), Q95 (≠ K103), K97 (≠ R105), H128 (= H136)

5t3oA Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus (see paper)
34% identity, 94% coverage: 5:301/317 of query aligns to 1:285/307 of 5t3oA

query
sites
5t3oA

P

P

P

L

L

L

L

I

F

F

A

S

L

G

Q

Q

G

S

T

N

Q

R

E

P

Y

L

A

A

A

Q

R

A

V

I

A

A

R

E

H

A

M

L

G

G

L

L

E

P

L

L

A

G

E

K

I

S

E

T

R

L

A

R

F

F

E

A

D

N

G

D

E

N

H

L

K

F

S

V

R

R

A

Y

L

E

T

E

P
x
S

V

L

E
x
R

G

E

R

G

K

D

V

V

A

F

V

I

F

V

H

Q

A

S

L

F

Y

V

G

P

D

-

E

-

R

-

S

P

V

V

N

Q

D

D

K

H

L

L

C

M

R

E

L

L

F

M

C

V

G

D

A

A

L

A

K

K

D

G

A

A

G

S

A

A

R

A

H

R

V

V

Q

T

V

A

V

V

A

I

P

P

Y

Y

L

F

C

S

Y

Y

A

A

R
|
R

K
x
S

E

D

R

K

T
x
D

Q

A

F

P

Q

R

D

I

P

S

I

I

I

T

T

A

R

R

Y

L

V

I

A

A

A

D

L

L

F

L

E

Q

A

T

C

A

R

G

V

A

D

D

R

R

L

V

T

L

T

T

L

M

E

T

V

L

H
|
H

N

S

L

-

A

P

A

Q

F

V

D

H

N

G

A

F

F

F

R

K

I

I

P

P

T

V

R

D

H

H

I

L

E

S

S

A

A

E

E

P

L

V

F

I

A

A

A

N

H

Y

F

F

A

A

Q

T

L

R

S

V

A

D

D

L

A

E

E

N

L

A

V

V

A

V

V

V

S

A

P

P

D

D

A

A

G

G

G

D

A

L

K

K

R

R

A

A

E

S

S

A

F

L

R

A

R

R

A

R

L

L

Q

-

H

-

T

-

G

-

R

-

S

G

V

L

G

P

S

L

A

A

Y

F

M

I

E

D

K

K

Y

E

R
|
R

S

V

N

S

D

D

I

T

-

E

V

V

T

R

G

V

T

R

M

M

L

L

V

V

G

G

D

E

V

V

R

E

D

G

R

K

I

T

A

A

I

L

I

I

V

V

D

D

L

E

I

I

S

S

T

T

G

A

G

G

T

S

L

L

L

V

R

E

A

A

G

V

E

E

A

A

C

L

R

M

K

Q

A

A

G

G

A

A

R

K

Q

E

V

V

H

Y

A

A

A

T

H

H

G

G

L

V

F

Y

T

V

G

-

G

G

P

P

E

A

L

L

-

D

-

R

-

I

L

A

E

K

S

S

P

P

V

V

F

-

D

K

Q

E

L

V

V

A

I

A

T

T

D

D

T

T

V

C

P

P

F

-

R

K

L

E

D

G

P

P

D

K

L

L

A

-

G

-

R

R

R

T

L

L

T

T

V

V

binding adenosine-5'-diphosphate: R94 (= R102), S95 (≠ K103), D99 (≠ T107), H128 (= H136), R193 (= R208)
binding sulfate ion: S45 (≠ P49), R47 (≠ E51)

7pn0A Crystal structure of the phosphorybosylpyrophosphate synthetase ii from thermus thermophilus at r32 space group
34% identity, 94% coverage: 5:301/317 of query aligns to 2:286/312 of 7pn0A

query
sites
7pn0A

P

P

P

L

L

L

L

I

F

F

A

S

L

G

Q

Q

G

S

T

N

Q

R

E

P

Y

L

A

A

A

Q

R

A

V

I

A

A

R

E

H

A

M

L

G

G

L

L

E

P

L

L

A

G

E

K

I

S

E

T

R

L

A

R

F

F

E

A

D

N

G

D

E

N

H

L

K

F

S

V

R

R

A

Y

L

E

T

E

P

S

V

L

E

R

G

E

R

G

K

D

V

V

A

F

V

I

F

V

H

Q

A

S

L

F

Y

V

G

P

D

-

E

-

R

-

S

P

V

V

N

Q

D

D

K

H

L

L

C

M

R

E

L

L

F

M

C

V

G

D

A

A

L

A

K

K

D

G

A

A

G

S

A

A

R

A

H

R

V

V

Q

T

V

A

V

V

A

I

P

P

Y

Y

L

F

C

S

Y

Y

A

A

R
|
R

K
x
S

E

D

R

K

T

D

Q

A

F

P

Q

R

D

I

P

S

I

I

I

T

T

A

R

R

Y

L

V

I

A

A

A

D

L

L

F

L

E

Q

A

T

C

A

R

G

V

A

D

D

R

R

L

V

T

L

T

T

L

M

E

T

V

L

H
|
H

N

S

L

-

A

P

A

Q

F

V

D

H

N

G

A

F

F

F

R

K

I

I

P

P

T

V

R

D

H

H

I

L

E

S

S

A

A

E

E

P

L

V

F

I

A

A

A

N

H

Y

F

F

A

A

Q

T

L

R

S

V

A

D

D

L

A

E

E

N

L

A

V

V

A

V

V

V

S

A

P

P

D

D

A

A

G

G

G

D

A

L

K

K

R

R

A

A

E

S

S

A

F

L

R

A

R

R

A

R

L

L

Q

-

H

-

T

-

G

-

R

-

S

G

V

L

G

P

S

L

A

A

Y

F

M

I

E

D

K

K

Y

E

R

R

S

V

N

S

D

D

I

T

-

E

V

V

T

R

G

V

T

R

M

M

L

L

V

V

G

G

D

E

V

V

R

E

D

G

R

K

I

T

A

A

I

L

I

I

V

V

D

D

L

E

I

I

S

S

T

T

G

A

G

G

T

S

L

L

L

V

R

E

A

A

G

V

E

E

A

A

C

L

R

M

K

Q

A

A

G

G

A

A

R

K

Q

E

V

V

H

Y

A

A

A

T

H

H

G

G

L

V

F

Y

T

V

G

G

G

P

P

A

-

L

-

D

E

R

L

I

L

A

E

K

S

S

P

P

V

V

F

-

D

K

Q

E

L

V

V

A

I

A

T

T

D

D

T

T

V

C

P

P

F

-

R

K

L

E

D

G

P

P

D

K

L

L

A

-

G

-

R

R

R

T

L

L

T

T

V

V

binding adenosine-5'-diphosphate: R95 (= R102), S96 (≠ K103), H129 (= H136)

7xmvA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a(amp/adp) filament bound with adp, amp and r5p (see paper)
32% identity, 96% coverage: 8:312/317 of query aligns to 4:292/307 of 7xmvA

query
sites
7xmvA

L

L

F

F

A

A

L

G

Q

N

G

A

T

T

Q

P

E

E

Y

L

A

A

A

Q

R

R

V

I

A

A

R

N

H

R

M

L

G

Y

L

T

E

S

L

L

A

G

E

D

I

A

E

A

E

V

R

G

A

R

F
|
F

E

S

D
|
D

G

G

E
|
E

H

V

K

S

S

V

R

Q

A

I

L

N

T

E

P

N

V

V

E

R

G

G

R

G

K

D

V

I

A

F

V

I

F

I

H

Q

A

S

L

T

Y

C

G

A

D

-

E

-

R

-

S

P

V

T

N

N

D

D

K

N

L

L

C

M

R

E

L

L

L

V

F

V

F

M

C

V

G

D

A

A

L

L

K

R

D

R

A

A

G

S

A

A

R

G

H

R

V

I

Q

T

V

A

V

V

A

I

P

P

Y

Y

L

F

C

G

Y

Y

A

A

R
|
R

K

Q

E

D

R
|
R

R

R

T
x
V

Q
x
R

F

S

-

A

Q

R

D

V

P

P

I

I

I

T

T

A

R

K

Y

V

V

V

A

A

A

D

L

F

F

L

E

S

A

S

C

V

R

G

V

V

D

D

R

R

L

V

T

L

T

T

L

V

E

D

V

L

H
|
H

-

A

-
x
E

N

Q

L

I

A

Q

A

G

F

F

D

-

N

-

A

-

F

F

R

D

I

V

P

P

T

V

R

D

H

N

I

V

E
x
F

S

G

A
x
S

E

P

L

I

F

L

A

L

A

E

H

D

F

M

A

L

Q

Q

L

L

S

N

A

L

D

D

A

N

E

P

L

I

V

V

A

-

V

V

S

S

P

P

D

D

A

I

G

G

A

V

K
x
V

R

R

A

A

E

R

S
x
A

F

I

R

A

R

K

A

L

L

L

Q

N

Q

D

H

-

T

-

G

-

R

-

S

-

V

T

G

D

S

M

A

A

Y

I

M

I

E

D

K

K

Y

R

R

R

S

Q

N

-

D

-

I

-

V

-

T

-

G

V

T

M

M

H

L

I

V

I

G

G

D

D

V

V

R

A

D

G

R

R

I

D

A

C

I

V

I

L

V

V

D
|
D

L
x
M

I

I

S
x
D

T
|
T

G

G

G
|
G

T
|
T

L

L

L

C

R

K

A

A

G

A

E

E

A

A

C

L

R

K

K

E

A

R

G

G

A

A

R

K

Q

R

V

V

H

F

A

A

A

Y

A

A

A

T

H

H

G

P

L

I

F

F

T

S

G

G

G

N

-

A

P

A

E

N

L

N

L

L

E

R

S

N

P

S

V

V

F

I

D

D

Q

E

L

V

V

V

I

V

T

C

D

D

T

T

V

I

P

P

P

L

F

S

R

D

L

E

D

I

P

K

D

S

L

L

A

P

G

N

R

V

R

R

L

T

T

L

V

T

L

L

D

-

S

-

S

S

A

G

M

M

L

L

A

A

A

E

A

A

L

I

binding adenosine-5'-diphosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R102), R97 (= R105), H129 (= H136)
binding adenosine monophosphate: R97 (= R105), V99 (≠ T107), R100 (≠ Q108), E131 (vs. gap), F145 (≠ E153), S147 (≠ A155), V173 (≠ K182), A177 (≠ S186)
binding 5-O-phosphono-alpha-D-ribofuranose: D212 (= D231), D213 (= D232), M214 (≠ L233), D216 (≠ S235), T217 (= T236), G219 (= G238), T220 (= T239)

7xmuA E.Coli phosphoribosylpyrophosphate (prpp) synthetase type a filament bound with adp, pi and r5p (see paper)
32% identity, 96% coverage: 8:312/317 of query aligns to 4:292/307 of 7xmuA

query
sites
7xmuA

L

L

F

F

A

A

L

G

Q

N

G

A

T

T

Q

P

E

E

Y

L

A

A

A

Q

R

R

V

I

A

A

R

N

H

R

M

L

G

Y

L

T

E

S

L

L

A

G

E

D

I

A

E

A

E

V

R

G

A

R

F
|
F

E

S

D
|
D

G

G

E
|
E

H

V

K

S

S

V

R

Q

A

I

L

N

T

E

P

N

V

V

E

R

G

G

R

G

K

D

V

I

A

F

V

I

F

I

H

Q

A

S

L

T

Y

C

G

A

D

-

E

-

R

-

S

P

V

T

N

N

D

D

K

N

L

L

C

M

R

E

L

L

L

V

F

V

F

M

C

V

G

D

A

A

L

L

K

R

D

R

A

A

G

S

A

A

R

G

H

R

V

I

Q

T

V

A

V

V

A

I

P

P

Y

Y

L

F

C

G

Y

Y

A

A

R
|
R

K
x
Q

E

D

R
|
R

R

R

T

V

Q
x
R

F

S

-

A

Q

R

D

V

P

P

I

I

I

T

T

A

R

K

Y

V

V

V

A

A

A

D

L

F

F

L

E

S

A

S

C

V

R

G

V

V

D

D

R

R

L

V

T

L

T

T

L

V

E

D

V

L

H
|
H

-

A

-
x
E

N

Q

L

I

A

Q

A

G

F

F

D

-

N

-

A

-

F

F

R

D

I

V

P

P

T

V

R

D

H

N

I

V

E
x
F

S

G

A
x
S

E

P

L

I

F

L

A

L

A

E

H

D

F

M

A

L

Q

Q

L

L

S

N

A

L

D

D

A

N

E

P

L

I

V

V

A

-

V

V

S

S

P

P

D
|
D

A

I

G

G

A

V

K
x
V

R

R

A

A

E

R

S

A

F

I

R

A

R

K

A

L

L

L

Q

N

Q

D

H

-

T

-

G

-

R

-

S

-

V

T

G

D

S

M

A

A

Y

I

M

I

E

D

K

K

Y

R

R

R

S

Q

N

-

D

-

I

-

V

-

T

-

G

V

T

M

M

H

L

I

V

I

G

G

D

D

V

V

R

A

D

G

R

R

I

D

A

C

I

V

I

L

V

V

D
|
D

D

D

L
x
M

I

I

S
x
D

T
|
T

G

G

T

T

L

L

L

C

R

K

A

A

G

A

E

E

A

A

C

L

R

K

K

E

A

R

G

G

A

A

R

K

Q

R

V

V

H

F

A

A

A

Y

A

A

A

T

H

H

G

P

L

I

F

F

T

S

G

G

G

N

-

A

P

A

E

N

L

N

L

L

E

R

S

N

P

S

V

V

F

I

D

D

Q

E

L

V

V

V

I

V

T

C

D

D

T

T

V

I

P

P

P

L

F

S

R

D

L

E

D

I

P

K

D

S

L

L

A

P

G

N

R

V

R

R

L

T

T

L

V

T

L

L

D

-

S

-

S

S

A

G

M

M

L

L

A

A

A

E

A

A

L

I

binding adenosine-5'-diphosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R102), Q95 (≠ K103), R97 (= R105), R97 (= R105), R100 (≠ Q108), H129 (= H136), E131 (vs. gap), F145 (≠ E153), S147 (≠ A155), V173 (≠ K182)
binding 5-O-phosphono-alpha-D-ribofuranose: D168 (= D177), D212 (= D231), M214 (≠ L233), D216 (≠ S235), T217 (= T236)

O94413 Ribose-phosphate pyrophosphokinase 2; Phosphoribosyl pyrophosphate synthase 2; EC 2.7.6.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 90% coverage: 1:286/317 of query aligns to 1:279/321 of O94413

query
sites
O94413

M

M

S

A

N

S

H

N

P

S

P

I

L

K

L

I

F

F

A

A

L

G

Q

N

G

S

T

H

Q

P

E

E

Y

L

A

A

A

E

R

K

V

V

A

A

R

R

H

R

M

I

G

G

L

L

E

S

L

L

A

G

E

K

I

V

E

A

E

V

R

V

A

Q

F

Y

E

S

D

N

G

R

E

E

H

T

K

S

S

V

R

T

A

I

L

G

T

E

P

S

V

V

E

R

G

D

R

E

K

D

V

V

A

F

V

I

F

L

H

Q

A

T

L

G

Y

C

G

G

D

-

E

-

R

-

S

S

V

I

N

N

D

D

K

H

L

L

C

M

R

E

L

L

F

I

F

M

C

I

G

N

A

A

L

C

K

R

D

S

A

A

G

S

A

A

R

R

H

R

V

I

Q

T

V

A

V

I

A

I

P

P

Y

C

L

F

C

P

Y

Y

A

A

R

R

K

Q

E

D

R

K

T

D

Q

K

F

S

Q

R

D

A

P

P

I

I

I

T

T

A

R

R

Y

L

V

V

A

A

A

N

L

M

F

L

E

Q

A

T

C

A

R

G

V

C

D

N

R

H

L

I

T

I

T

T

L

M

E

D

V

L

H

H

N

-

L

A

A

S

A

Q

F

I

D

Q

N

G

A

F

F

F

R

N

I

V

P

P

T

V

R

D

H

N

I

L

E

Y

S

-

A

A

E

E

L

P

F

S

A

V

A

L

H

R

F

Y

A

I

Q

R

L

E

S

N

A

I

D

D

A

T

E

T

L

V

-

N

-

P

-

T

V

V

A

I

V

V

S
|
S

P

P

D

D

A

A

G

G

A

A

K

K

R

R

A

A

E

T

S

-

F

-

R

-

A

A

L

L

Q

A

Q

D

H

R

T

L

G

D

R

L

S

D

V

F

G

A

S

L

A

I

Y

H

M

K

E

E

K

R

Y

Q

R

K

S

A

N

N

D

E

I

-

V

V

T

S

G

R

T

M

M

V

L

L

V

V

G

G

D

D

V

V

R

R

D

D

R

K

I

L

A

A

I

I

I

L

V

V

D

D

L

M

I

A

S

D

T

T

G

C

G

G

T

T

L

L

L

G

R

L

A

A

G

A

E

K

A

T

C

L

R

K

K

D

A

N

G

G

A

A

R

K

Q

A

V

V

H

Y

A

A

A

I

A

V

A

T

H

H

G

G

L

I

F

L

T

S

G

G

-

K

G

A

P

I

E

K

L

V

L

I

E

N

S

E

P

S

V

A

F

L

D

E

Q

K

L

V

V

I

I

V

T

T

D

N

T

T

V

I

P

P

S172 (= S175) modified: Phosphoserine

8dbgA Human prps1 with phosphate and atp; hexamer (see paper)
30% identity, 96% coverage: 8:312/317 of query aligns to 5:292/309 of 8dbgA

query
sites
8dbgA

L

I

F

F

A

S

L

G

Q

S

G

S

T

H

Q

Q

E

D

Y

L

A

S

A

Q

R

K

V

I

A

A

R

D

H

R

M

L

G

G

L

L

E

E

L

L

A

G

E

K

I

V

E

V

E

T

R

K

A

K

F
|
F

E

S

D
x
N

G

Q

E
|
E

H

T

K

C

S

V

R

E

A

I

L

G

T

E

P
x
S

V

V

E
x
R

G

G

R

E

K

D

V

V

A

Y

V

I

F

V

H

Q

A

S

L

G

Y

C

G

G

D

E

D

-

E

-

R

-

S

-

V

I

N

N

D

D

K

N

L

L

C

M

R

E

L

L

F

I

F

M

C

I

G

N

A

A

L

C

K

K

D

I

A

A

G

S

A

A

R

S

H

R

V

V

Q

T

V

A

V

V

A

I

P

P

Y

C

L

F

C

P

Y

Y

A

A

R
|
R

K
x
Q

E

D

R
x
K

R

K

T

D

Q

K

F

S

Q

R

D

A

P

P

I

I

I

S

T

A

R

K

Y

L

V

V

A

A

A

N

L

M

F

L

E

S

A

V

C

A

R

G

V

A

D

D

R

H

L

I

T

I

T

T

L

M

E

D

V

L

H
|
H

N

-

L

A

A

S

A

Q

F

I

D

Q

N

G

A

F

F

F

R

D

I

I

P

P

T

V

R

D

H

N

I

L

E

Y

S

A

A

E

E

P

L

A

F

V

A

L

A

K

H

W

F

I

A

R

Q

E

-

N

L

I

S

S

A

E

D

W

A

R

E

N

L

C

V

T

A

I

V

V

S

S

P

P

D

D

A

A

G

G

A

A

K

K

R

R

A

V

E

T

S

S

F

I

R

A

R

D

A

R

L

L

Q

-

H

-

T

-

G

-

R

-

S

N

V

V

G

D

S

F

A

A

Y

L

M

I

E

H

K

K

Y

E

R

D

S

R

N

-

D

-

I

-

V

-

T

-

G

-

T

M

M

V

L

L

V

V

G

G

D

D

V

V

R

K

D

D

R

R

I

V

A

A

I

I

I

L

V

V

D

D

L

M

I

A

S
x
D

T
|
T

G
x
C

G

G

T
|
T

L

I

L

C

R

H

A

A

G

A

E

D

A

K

C

L

R

L

K

S

A

A

G

G

A

A

R

T

Q

R

V

V

H

Y

A

A

A

I

A

L

A

T

H

H

G

G

L

I

F

F

T

S

G

-

G

G

P

P

E

A

L

I

-

S

-

R

L

I

E

N

S

N

P

A

V

C

F

F

D

E

Q

A

L

V

V

V

I

V

T

T

D

N

T

T

V

I

P

P

P

-

F

-

R

Q

L

E

D

D

P

K

D

M

L

K

A

H

G

C

R

S

R

K

L

I

T

Q

V

V

L

I

D

D

S

I

S

S

A

M

M

I

L

L

A

A

A

E

A

A

L

I

binding adenosine-5'-triphosphate: F34 (= F37), N36 (≠ D39), E38 (= E41), R95 (= R102), Q96 (≠ K103), K98 (≠ R105), H129 (= H136)
binding phosphate ion: S46 (≠ P49), R48 (≠ E51), D216 (≠ S235), T217 (= T236), C218 (≠ G237), T220 (= T239)

Q63XL8 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Burkholderia pseudomallei (strain K96243) (see paper)
30% identity, 97% coverage: 1:309/317 of query aligns to 1:299/318 of Q63XL8

query
sites
Q63XL8

M

M

S

S

N

S

H

H

P

D

P

G

L

L

L

M

-

V

F

F

A

T

L

G

Q

N

G

A

T

N

Q

P

E

A

Y

L

A

A

A

Q

R

E

V

V

A

V

R

K

H

I

M

L

G

G

L

I

E

P

L

L

A

G

E

K

I

A

E

M

E

V

R

S

A

R

F

F

E

S

D

D

G

G

E

E

H

I

K

Q

S

V

R

E

A

I

L

Q

T

E

P

N

V

V

E

R

G

G

R

K

K

D

V

V

A

F

V

V

F

L

H

Q

A

S

L

T

Y

C

G

A

D

-

E

-

R

-

S

P

V

T

N

N

D

D

K

N

L

L

C

M

R

E

L

L

L

M

F

I

F

M

C

V

G

D

A

A

L

L

K

K

D

R

A

A

G

S

A

A

R

G

H

R

V

I

Q

T

V

A

A

I

P

P

Y

Y

L

F

C

G

Y

Y

A

A

R
|
R

K
x
Q

E

D

R

R

T

P

Q

R

F

S

Q

A

D

R

P

V

I

A

I

I

T

S

-

A

R

K

Y

V

V

V

A

A

A

N

L

M

F

L

E

E

A

I

C

A

R

G

V

V

D

E

R

R

L

I

T

I

T

T

L

M

E

D

V

L

H

H

-

A

-

D

N

Q

L

I

A

Q

A

G

F

F

D

-

N

-

A

-

F

F

R

D

I

I

P

P

T

V

R

D

H

N

I

I

E

Y

S

A

A

T

E

P

L

I

F

L

A

L

A

G

H

D

F

L

A

R

Q

K

L

Q

S

N

A

Y

D

-

A

P

E

D

L

L

V

L

A

V

V

V

S

S

P

P

D

D

A

V

G

G

A

V

K

V

R

R

A

A

E

R

S

A

F

L

R

A

R

K

A

Q

L

L

Q

N

Q

C

H

D

T

L

G

-

R

-

S

-

V

-

G

-

S

-

A

A

Y

I

M

I

E

D

K

K

Y

R

R

R

S

P

N

K

D

A

I

N

V

V

T

A

G

E

T

V

M

M

-

N

L

I

V

I

G

G

D

E

V

V

R

E

D

G

R

R

I

T

A

C

I

V

I

I

V

M

D

D

L

M

I

V

S

D

T

T

G

A

G

G

T

T

L

L

L

C

R

K

A

A

G

A

E

Q

A

V

C

L

R

K

K

E

A

R

G

G

A

A

R

K

Q

Q

V

V

H

F

A

A

A

Y

A

A

A

T

H

H

G

P

L

V

F

L

T

S

G

G

-

A

P

A

E

D

L

R

L

I

E

A

S

A

P

S

V

A

F

L

D

D

Q

E

L

L

V

V

I

V

T

T

D

D

T

T

V

I

P

P

P

L

F

S

R

A

L

-

D

-

P

E

D

S

L

L

A

A

G

C

R

P

R

K

L

I

T

R

V

A

L

L

D

S

S

S

S

A

A

G

M

L

L

L

A

A

RQ 99:100 (≠ RK 102:103) binding

P14193 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PPRibP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Bacillus subtilis (strain 168) (see 4 papers)
27% identity, 96% coverage: 8:312/317 of query aligns to 12:303/317 of P14193

query
sites
P14193

L

I

F

F

A

S

L

L

Q

N

G

S

T

N

Q

P

E

E

Y

L

A

A

A

K

R

E

V

I

A

A

R

D

H

I

M

V

G

G

L

V

E

Q

L

L

A

G

E

K

I

C

E

S

E

V

R

T

A

R

F

F

E

S

D

D

G

G

E

E

H

V

K

Q

S

I

R

N

A

I

L

E

T

E

P

S

V

I

E

R

G

G

R

C

K

D

V

C

A

Y

V

I

F

I

H

Q

A

S

L

-

Y

-

G

-

D

-

D

T

E

S

R

D

S

P

V

V

N

N

D

E

K

H

L

I

C

M

R

E

L

L

F

I

F

M

C

V

G

D

A

A

L

L

K

K

D

R

A

A

G

S

A

A

R

K

H

T

V

I

Q

N

V

I

V

V

A

I

P

P

Y

Y

L

Y

C

G

Y

Y

A

A

R
|
R

K
x
Q

E

D

R

R

R

K

T

A

Q

R

F

S

Q

R

D

E

P

P

I

I

I

T

T

A

R

K

Y

L

V

F

A

A

A

N

L

L

F

L

E

E

A

T

C

A

R

G

V

A

D

T

R

R

L

V

T

I

T

A

L

L

E

D

V

L

H

H

N

-

L

A

A

P

A

Q

F

I

D

Q

N

G

A

F

F

F

R

D

I

I

P

P

T

I

R

D

H

H

I

L

E

M

S

G

A

V

E

P

L

I

F

L

A

G

A

E

H

Y

F

F

A

E

Q

G

L

K

S

N

A

L

D

E

A

-

E

D

L

I

V

V

A

I

V

V

S

S

P

P

D

D

A

H

G

G

A

V

K

T

R

R

A

A

E

R

S

K

F

L

R

A

R

D

A

R

L

L

Q

K

Q

A

H

P

T

I

G

-

R

-

S

-

V

-

G

-

S

-

A

A

Y

I

M

I

E

D

K
|
K

Y

R

R
|
R

S

P

N
x
R

D

P

I
x
N

V

V

T

A

G
x
E

T

V

M

M

-

N

L

I

V

V

G

G

D

N

V

I

R

E

D

G

R

K

I

T

A

A

I

I

I

L

V

I

D

D

L

I

I

I

S
x
D

T
|
T

G
x
A

G
|
G

T
|
T

L

I

L

T

R

L

A

A

G

A

E

N

A

A

C

L

R

V

K

E

A

N

G

G

A

A

R

K

Q

E

V

V

H

Y

A

A

A

C

A

T

H

H

G

P

L

V

F

L

T

S

G

G

-

P

G

A

P

V

E

E

L

R

L

I

E

N

S

N

P

S

V

T

F

I

D

K

Q

E

L

L

V

V

I

V

T

T

D

N

T

S

V

I

P

-

F

-

R

K

L

L

D

P

P

E

D

E

L

K

A

K

G

I

R

E

R

R

L

F

T

K

V

Q

L

L

D

S

S

V

S

G

A

P

M

L

L

L

A

A

A

E

A

A

L

I

RQ 102:103 (≠ RK 102:103) binding
K198 (= K206) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type. The cooperativity of ADP binding is reduced.
R200 (= R208) mutation to A: Strong decrease of the Vmax value compared to that of the wild-type enzyme. The affinity binding for ATP and Rib-5-P are slightly altered compared to the wild-type.
R202 (≠ N210) mutation to A: 3-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
N204 (≠ I212) mutation to A: 4.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
E207 (≠ G215) mutation to A: 2.5-fold decrease in the affinity binding for ATP. Slight decrease of the Vmax value.
DTAGT 228:232 (≠ STGGT 235:239) binding

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

3dahC 2.3 a crystal structure of ribose-phosphate pyrophosphokinase from burkholderia pseudomallei (see paper)
29% identity, 96% coverage: 7:309/317 of query aligns to 3:287/300 of 3dahC

query
sites
3dahC

L

M

L

V

F

F

A

T

L

G

Q

N

G

A

T

N

Q

P

E

A

Y

L

A

A

A

Q

R

E

V

V

A

V

R

K

H

I

M

L

G

G

L

I

E

P

L

L

A

G

E

K

I

A

E

M

E

V

R

S

A

R

F
|
F

E

S

D
|
D

G

G

E
|
E

H

I

K

Q

S

V

R

E

A

I

L

Q

T

E

P

N

V

V

E

R

G

G

R

K

K

D

V

V

A

F

V

V

F

L

H

Q

A

S

L

T

Y

C

G

A

D

-

E

-

R

-

S

P

V

T

N

N

D

D

K

N

L

L

C

M

R

E

L

L

L

M

F

I

F

M

C

V

G

D

A

A

L

L

K

K

D

R

A

A

G

S

A

A

R

G

H

R

V

I

Q

T

V

A

A

I

P

P

Y

Y

L

F

C

G

Y

Y

A

A

R
|
R

K
x
Q

E

D

R

R

T

P

Q

R

F

S

Q

A

D

R

P

V

I

A

I

I

T

S

-

A

R

K

Y

V

V

V

A

A

A

N

L

M

F

L

E

E

A

I

C

A

R

G

V

V

D

E

R

R

L

I

T

I

T

T

L

M

E

D

V

L

H

H

-

A

-

D

N

Q

L

I

A

Q

A

G

F

F

D

-

N

-

A

-

F

F

R

D

I

I

P

P

T

V

R

D

H

N

I

I

E

Y

S

A

A

T

E

P

L

I

F

L

A

L

A

G

H

D

F

L

A

R

Q

K

L

Q

S

N

A

Y

D

-

A

P

E

D

L

L

V

L

A

V

V

V

S

S

P

P

D

D

A

V

G

G

A

V

K

V

R

R

A

A

E

R

S

A

F

L

R

A

R

K

A

Q

L

L

Q

N

Q

C

H

D

T

L

G

-

R

-

S

-

V

-

G

-

S

-

A

A

Y

I

M

I

E

D

K

K

Y

R

R

R

S

V

N

M

D

N

I

I

V

I

T

-

G

-

T

-

M

-

L

-

V

-

G

G

D

E

V

V

R

E

D

G

R

R

I

T

A

C

I

V

I

I

V

M

D

D

L

M

I

V

S

D

T

T

G

A

G

G

T

T

L

L

L

C

R

K

A

A

G

A

E

Q

A

V

C

L

R

K

K

E

A

R

G

G

A

A

R

K

Q

Q

V

V

H

F

A

A

A

Y

A

A

A

T

H

H

G

P

L

V

F

L

T

S

G

G

-

A

P

A

E

D

L

R

L

I

E

A

S

A

P

S

V

A

F

L

D

D

Q

E

L

L

V

V

I

V

T

T

D

D

T

T

V

I

P

P

P

L

F

S

R

A

L

-

D

-

P

E

D

S

L

L

A

A

G

C

R

P

R

K

L

I

T

R

V

A

L

L

D

S

S

S

S

A

A

G

M

L

L

L

A

A

binding adenosine monophosphate: F33 (= F37), D35 (= D39), E37 (= E41), R94 (= R102), Q95 (≠ K103)

1dkuA Crystal structures of bacillus subtilis phosphoribosylpyrophosphate synthetase: molecular basis of allosteric inhibition and activation. (see paper)
28% identity, 88% coverage: 8:285/317 of query aligns to 4:262/295 of 1dkuA

query
sites
1dkuA

L

I

F

F

A

S

L

L

Q

N

G

S

T

N

Q

P

E

E

Y

L

A

A

A

K

R

E

V

I

A

A

R

D

H

I

M

V

G

G

L

V

E

Q

L

L

A

G

E

K

I

C

E

S

E

V

R

T

A

R

F

F

E

S

D

D

G

G

E

E

H

V

K

Q

S

I

R

N

A

I

L

E

T

E

P

S

V

I

E

R

G

G

R

C

K

D

V

C

A

Y

V

I

F

I

H

Q

A

S

L

-

Y

-

G

-

D

-

D

T

E

S

R

D

S

P

V

V

N

N

D

E

K

H

L

I

C

M

R

E

L

L

F

I

F

M

C

V

G

D

A

A

L

L

K

K

D

R

A

A

G

S

A

A

R

K

H

T

V

I

Q

N

V

I

V

V

A

I

P

P

Y

Y

L

Y

C

G

Y

Y

A

A

R
|
R

K

Q

E

D

R

R

R
x
K

T

A

Q

R

F
x
S

Q
x
R

D

E

P

P

I

I

I

T

T

A

R

K

Y

L

V

F

A

A

A

N

L

L

F

L

E

E

A

T

C

A

R

G

V

A

D

T

R

R

L

V

T

I

T

A

L

L

E

D

V

L

H
|
H

-

A

-

P

N

Q

L

I

A

Q

A

G

F

F

D

-

N

-

A

-

F

F

R

D

I

I

P

P

T

I

R
x
D

H

H

I

L

E

M

S

G

A

V

E

P

L

I

F

L

A

G

A

E

H

Y

F

F

A

E

Q

G

L

K

S

N

A

L

D

E

A

-

E

D

L

I

V

V

A

I

V

V

S

S

P

P

D

D

A

H

G

G

A

V

K

T

R

R

A

A

E

R

S

K

F

L

R

A

R

D

A

R

L

L

Q

K

Q

A

H

P

T

I

G

-

R

-

S

-

V

-

G

-

S

-

A

A

Y

I

M

I

E

D

K

K

Y

-

R

R

S

M

N

N

D

-

I

-

V

-

T

-

G

-

T

-

M

-

L

I

V

V

G

G

D

N

V

I

R

E

D

G

R

K

I

T

A

A

I

I

I

L

V

I

D

D

L

I

I

I

S

D

T

T

G

A

G

G

T

T

L

I

L

T

R

L

A

A

G

A

E

N

A

A

C

L

R

V

K

E

A

N

G

G

A

A

R

K

Q

E

V

V

H

Y

A

A

A

C

A

T

H

H

G

P

L

V

F

L

T

S

G

G

-

P

G

A

P

V

E

E

L

R

L

I

E

N

S

N

P

S

V

T

F

I

D

K

Q

E

L

L

V

V

I

V

T

T

D

N

T

S

V

I

binding methyl phosphonic acid adenosine ester: R94 (= R102), H128 (= H136)
binding phosphomethylphosphonic acid adenosyl ester: K98 (≠ R106), S101 (≠ F109), R102 (≠ Q110), D141 (≠ R150)

Sites not aligning to the query:

binding phosphomethylphosphonic acid adenosyl ester: 290, 291, 292, 295

P9WKE3 Ribose-phosphate pyrophosphokinase; RPPK; 5-phospho-D-ribosyl alpha-1-diphosphate synthase; Phosphoribosyl diphosphate synthase; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase; PRPPase; EC 2.7.6.1 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
29% identity, 98% coverage: 7:316/317 of query aligns to 13:313/326 of P9WKE3

query
sites
P9WKE3

L

M

L

L

F

F

A

A

L

G

Q

R

G

A

T

H

Q

P

E

E

Y

L

A

A

A

E

R

Q

V

V

A

A

R
x
K

H

E

M

L

G

D

L

V

E

H

L

V

A

T

E

S

I

Q

E

D

E

A

R

R

A

E

F

F

E

A

D

N

G

G

E

E

H

I

K

F

S

V

R

R

A

F

L

H

T

E

P

S

V

V

E

R

G

G

R

C

K

D

V

A

A

F

V

V

F

L

H

Q

A

S

L

C

Y

P

G

A

D

-

E

-

R

-

S

P

V

V

N

N

D

R

K

W

L

L

C

M

R

E

L

Q

L

L

F

I

F

M

C

I

G

D

A

A

L

L

K

K

D

R

A

G

G

S

A

A

R

K

H

R

V

I

Q

T

V

A

V

V

A

M

P

P

Y

F

L

Y

C

P

Y

Y

A

A

R

R

K

Q

E

D

R

K

T

H

Q

R

F

G

Q

R

D

E

P

P

I

I

I

S

T

A

R

R

Y

L

V

I

A

A

A

D

L

L

F

L

E

K

A

T

C

A

R

G

V

A

D

D

R

R

L

I

T

V

T

T

L

V

E

D

V

L

H

H

N

T

L

-

A

D

A

Q

F

I

D

Q

N

G

A

F

F

F

R

D

I

G

P

P

T

V

R

D

H

H

I

M

E

R

S

G

A

Q

E

N

L

L

F

L

A

T

A

G

H

Y

F

I

A

R

Q

D

L

N

S

Y

A

P

D

D

A

G

E

N

L

M

V

V

A

V

V

V

S

S

P

P

D

D

A

S

G

G

G

R

A

V

K

R

R

I

A

A

E

E

S

K

F

W

R

A

R

D

A

A

L

L

Q

G

Q

-

H

-

T

-

G

-

R

-

S

G

V

V

G

P

S

L

A

A

Y

F

M

I

E

H

K

K

Y

T

R

R

S

D

N

P

D

R

I

V

-

P

-

N

-

Q

V

V

T

V

G

S

T

N

M

R

L

V

V

V

G

G

D

D

V

V

R

A

D

G

R

R

I

T

A

C

I

V

I

L

V

I

D

D

L

M

I

I

S

D

T

T

G

G

T

T

L

I

L

A

R

G

A

A

G

V

E

A

A

L

C

L

R

H

K

N

A

D

G

G

A

A

R

G

Q

D

V

V

H

I

A

I

A

A

A

T

H

H

G

G

L

V

F

L

T

S

G

D

-

P

G

A

P

A

E

Q

L

R

L

L

E

A

S

S

P

C

V

G

F

A

D

R

Q

E

L

V

V

I

I

V

T

T

D

N

T

T

V

L

P

P

P

-

F

-

R

-

L

I

D

G

P

E

D

D

L

K

A

R

G

F

R

P

R

Q

L

L

T

T

V

V

L

L

D

S

S

I

S

A

A

P

M

L

L

L

A

A

A

S

A

T

L

I

A

R

A

A

E

V

Y

F

K29 (≠ R23) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

1ibsB Phosphoribosyldiphosphate synthetase in complex with cadmium ions (see paper)
27% identity, 96% coverage: 8:312/317 of query aligns to 6:286/299 of 1ibsB

query
sites
1ibsB

L

I

F

F

A

S

L

L

Q

N

G

S

T

N

Q

P

E

E

Y

L

A

A

A

K

R

E

V

I

A

A

R

D

H

I

M

V

G

G

L

V

E

Q

L

L

A

G

E

K

I

C

E

S

E

V

R

T

A

R

F

F

E

S

D

D

G

G

E

E

H

V

K

Q

S

I

R

N

A

I

L

E

T

E

P

S

V

I

E

R

G

G

R

C

K

D

V

C

A

Y

V

I

F

I

H

Q

A

S

L

-

Y

-

G

-

D

-

D

T

E

S

R

D

S

P

V

V

N

N

D

E

K

H

L

I

C

M

R

E

L

L

F

I

F

M

C

V

G

D

A

A

L

L

K

K

D

R

A

A

G

S

A

A

R

K

H

T

V

I

Q

N

V

I

V

V

A

I

P

P

Y

Y

L

Y

C

G

Y

Y

A

A

R
|
R

K
x
Q

E

D

R
|
R

R

K

T

S

Q

R

F

-

Q

-

D

E

P

P

I

I

I

T

T

A

R

K

Y

L

V

F

A

A

A

N

L

L

F

L

E

E

A

T

C

A

R

G

V

A

D

T

R

R

L

V

T

I

T

A

L

L

E

D

V

L

H

H

-

A

-

P

N

Q

L

I

A

Q

A

G

F

F

D

-

N

-

A

-

F

F

R

D

I

I

P

P

T

I

R

D

H

H

I

L

E

M

S

G

A

V

E

P

L

I

F

L

A

G

A

E

H

Y

F

F

A

E

Q

G

L

K

S

N

A

L

D

E

A

-

E

D

L

I

V

V

A

I

V

V

S

S

P

P

D

D

A

H

G

G

A

V

K

T

R

R

A

A

E

R

S

K

F

L

R

A

R

D

A

R

L

L

Q

K

Q

A

H

P

T

I

G

-

R

-

S

-

V

-

G

-

S

-

A

A

Y

I

M

I

E

D

K

K

Y

-

R

R

S

M

N

N

D

-

I

-

V

-

T

-

G

-

T

-

M

-

L

I

V

V

G

G

D

N

V

I

R

E

D

G

R

K

I

T

A

A

I

I

I

L

V

I

D

D

L

I

I

I

S

D

T

T

G

A

G

G

T

T

L

I

L

T

R

L

A

A

G

A

E

N

A

A

C

L

R

V

K

E

A

N

G

G

A

A

R

K

Q

E

V

V

H

Y

A

A

A

C

A

T

H

H

G

P

L

V

F

L

T

S

G

G

-

P

G

A

P

V

E

E

L

R

L

I

E

N

S

N

P

S

V

T

F

I

D

K

Q

E

L

L

V

V

I

V

T

T

D

N

T

S

V

I

P

-

F

-

R

K

L

L

D

P

P

E

D

E

L

K

A

K

G

I

R

E

R

R

L

F

T

K

V

Q

L

L

D

S

S

V

S

G

A

P

M

L

L

L

A

A

A

E

A

A

L

I

binding methyl phosphonic acid adenosine ester: R96 (= R102), Q97 (≠ K103), R99 (= R105)

Query Sequence

>GFF2178 FitnessBrowser__psRCH2:GFF2178
MSNHPPLLFALQGTQEYAARVARHMGLELAEIEERAFEDGEHKSRALTPVEGRKVAVFHA
LYGDDERSVNDKLCRLLFFCGALKDAGARHVQVVAPYLCYARKERRTQFQDPIITRYVAA
LFEACRVDRLTTLEVHNLAAFDNAFRIPTRHIESAELFAAHFAQLSADAELVAVSPDAGG
AKRAESFRRALQQHTGRSVGSAYMEKYRSNDIVTGTMLVGDVRDRIAIIVDDLISTGGTL
LRAGEACRKAGARQVHAAAAHGLFTGGPELLESPVFDQLVITDTVPPFRLDPDLAGRRLT
VLDSSAMLAAALAAEYR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory