Comparing GFF2253 FitnessBrowser__Marino:GFF2253 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 18 hits to proteins with known functional sites (download)
1l5jA Crystal structure of e. Coli aconitase b. (see paper)
72% identity, 99% coverage: 1:858/864 of query aligns to 1:856/862 of 1l5jA
P36683 Aconitate hydratase B; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; 2-methyl-cis-aconitate hydratase; Iron-responsive protein-like; IRP-like; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Escherichia coli (strain K12) (see 2 papers)
72% identity, 99% coverage: 1:858/864 of query aligns to 1:856/865 of P36683
4kp1A Crystal structure of ipm isomerase large subunit from methanococcus jannaschii (mj0499) (see paper)
28% identity, 52% coverage: 380:825/864 of query aligns to 1:420/423 of 4kp1A
4nqyA The reduced form of mj0499 (see paper)
26% identity, 52% coverage: 381:825/864 of query aligns to 1:407/409 of 4nqyA
O14289 3-isopropylmalate dehydratase; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
25% identity, 52% coverage: 397:849/864 of query aligns to 54:496/758 of O14289
P39533 Homocitrate dehydratase, mitochondrial; Aconitase 2; EC 4.2.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
26% identity, 51% coverage: 381:820/864 of query aligns to 54:505/789 of P39533
Sites not aligning to the query:
P19414 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
26% identity, 49% coverage: 401:822/864 of query aligns to 57:502/778 of P19414
Sites not aligning to the query:
1b0kA S642a:fluorocitrate complex of aconitase (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 117:477/753 of 1b0kA
Sites not aligning to the query:
P16276 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Sus scrofa (Pig) (see 3 papers)
25% identity, 42% coverage: 457:822/864 of query aligns to 145:505/781 of P16276
Sites not aligning to the query:
5acnA Structure of activated aconitase. Formation of the (4fe-4s) cluster in the crystal (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 118:478/754 of 5acnA
Sites not aligning to the query:
8acnA Crystal structures of aconitase with isocitrate and nitroisocitrate bound (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 117:477/753 of 8acnA
Sites not aligning to the query:
1nisA Crystal structure of aconitase with trans-aconitate and nitrocitrate bound (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 117:477/753 of 1nisA
Sites not aligning to the query:
1fghA Complex with 4-hydroxy-trans-aconitate (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 117:477/753 of 1fghA
Sites not aligning to the query:
1amjA Steric and conformational features of the aconitase mechanism (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 117:477/753 of 1amjA
Sites not aligning to the query:
1amiA Steric and conformational features of the aconitase mechanism (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 117:477/753 of 1amiA
Sites not aligning to the query:
1acoA Crystal structure of aconitase with transaconitate bound (see paper)
25% identity, 42% coverage: 457:822/864 of query aligns to 117:477/753 of 1acoA
Sites not aligning to the query:
P20004 Aconitate hydratase, mitochondrial; Aconitase; Citrate hydro-lyase; EC 4.2.1.3 from Bos taurus (Bovine) (see 2 papers)
25% identity, 42% coverage: 457:822/864 of query aligns to 145:505/780 of P20004
Sites not aligning to the query:
A0QX20 Aconitate hydratase A; ACN; Aconitase; (2R,3S)-2-methylisocitrate dehydratase; (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate dehydratase; Iron-responsive protein-like; IRP-like; Probable 2-methyl-cis-aconitate hydratase; RNA-binding protein; EC 4.2.1.3; EC 4.2.1.99 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
25% identity, 30% coverage: 431:692/864 of query aligns to 126:381/943 of A0QX20
Sites not aligning to the query:
>GFF2253 FitnessBrowser__Marino:GFF2253
MLEAYREHVAEREALGIPPKPLNAEQTAALVDLLKNPPAGEEDTLVYLLENRVPPGVDEA
AYVKAAFLTAIVKGEASSPLLDKPTAVKLLGMMQGGYNIATLVDLLDDSELAELAGEQLK
RTLLMFDAFNDVKEKMDAGNPVARAVIESWANGEWFTNKKKVPESTKMVVFKVTGETNTD
DLSPAPDAWSRPDIPLHARAAYKMERDGLKPEEPGVTGPMSQIDEIKAKGLPVAFVGDVV
GTGSSRKSATNSVLWFFGDDIPGVPNKRAGGVCIGNKVAPIFFNTMEDAGALVFEAPVDD
MNMGDVIEIRPYDGKILNEAGETISEFKFKSDVILDEVQAGGRIPLIIGRGLTAKARTAL
GMGATDLFRLPKDPEAGTKGFTLAQKMVGKACGLEEGKGVRPNTYCEPHMTTVGSQDTTG
PMTRDELKDLACLGFQADLVMQSFCHTAAYPKPVDVEMQHTMPDFIRTRGGVSLRPGDGI
IHSWLNRMLLPDTVGTGGDSHTRFPMGISFPAGSGLVAFAAATGVMPLDMPESVLVRFKG
EMQPGITLRDLVHAIPLYGIKQGMLTVEKKGKINEFSGRILEIEGLEHLTVEQAFELSDA
SAERSAAGCTINLSEDSVAEYLRSNITMLRWMIAEGYGDPRTLERRAQQMEEWLADPKLM
RADKDAEYAHVIEIDLADIKEPIVCCPNDPDDAKFLSEVAGDKVDEVFIGSCMTNIGHFR
AAGKLLEQHKGPLSTRLWMSPPTKMDQAQLMEEGYFNTYGTAGVRTEMPGCSLCMGNQAR
VAPKSTVLSTSTRNFPNRLGDGANVYLTSAELAAVGAVLGKLPSPAEYMEYAKDLNSMSK
EIYKYLNFDQMENYTKKAAEANVA
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SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.
Lawrence Berkeley National Laboratory