SitesBLAST

R257 (= R251) mutation to C: Sodium-independent binding affinity for proline.
C281 (≠ S275) mutation to S: Does not affect proline uptake activity. Confers resistance to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
C344 (≠ S334) mutation to S: Small decrease in proline uptake activity. Confers resistance to N-ethylmaleimide. Exhibits low Na(+)-dependent proline binding.
C349 (≠ S339) mutation to S: Does not affect proline uptake activity. Sensitive to N-ethylmaleimide. Na(+)-dependent proline binding activity is similar to wild-type carrier.
R376 (= R366) mutation R->E,Q: No change in activity.; mutation to K: Loss of activity.

P11170 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Oryctolagus cuniculus (Rabbit) (see 2 papers)
23% identity, 70% coverage: 92:427/481 of query aligns to 126:492/662 of P11170

query
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P11170

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C255 (vs. gap) modified: Disulfide link with 608
Q457 (≠ A392) mutation to W: Drasticly decreased affinity for glucose and phlorizin.
T460 (≠ W395) mutation to W: Decreased affinity for glucose and phlorizin.

Sites not aligning to the query:

608 modified: Disulfide link with 255

Q9NY91 Probable glucose sensor protein SLC5A4; Solute carrier family 5 member 4 from Homo sapiens (Human) (see paper)
23% identity, 70% coverage: 92:429/481 of query aligns to 126:494/659 of Q9NY91

query
sites
Q9NY91

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E457 (≠ A392) mutation to Q: Confers sugar transport activity not found in the wild-type protein. Increased sensitivity to inhibitor phlorizin.

9bimA Cryo-em structure of human cht1 in the hc-3 bound outward-facing state (see paper)
24% identity, 79% coverage: 1:380/481 of query aligns to 17:393/516 of 9bimA

query
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9bimA

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binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): W59 (≠ D39), Y77 (≠ L57), Y88 (≠ I62), W138 (≠ F121), W251 (= W238)

Sites not aligning to the query:

binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): 404, 407

8zqpA High affinity choline transporter 1 (see paper)
24% identity, 79% coverage: 1:380/481 of query aligns to 15:393/470 of 8zqpA

query
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8zqpA

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F

A

A

P

K

R

P

L

M

R

R

E

S

Q

K

T

-

V

-

H

-

Y

-

G

-

N

G

A

Y

I

V

T

T

I

M

P

L

S

D

F

P

L

F

A

Q

N

Q

R

I

F

Y

P

G

T

K

Q

R

A

M

L

G

S

G

L

L

R

L

T

F

V

I

S

P

A

A

I

L

V

M

I

G

V

E

I

M

F

F

F

W

A

A

V

A

Y

A

T

I

A

F

S

S

G

A

L

L

V

G

A

A

G

-

K

-

L

-

F

-

E

-

S

-

A

T

F

I

S

S

G

V

I

I

F

I

N

D

F

V

G

-

L

-

S

-

D

D

Y

M

A

H

V

I

G

S

I

V

V

I

I

I

T

S

L

A

G

L

V

I

V

A

L

T

A

L

Y

Y

T

T

V

L

V

V

G

G

F

L

L

Y

A

S

V

V

S

A

L

Y

T

T

D
|
D

F

V

V

V

Q

Q

G

L

C

F

I

C

M

I

M

F

L

V

A

G

L

L

V

W

I

I

-

S

-

V

-

P

-

F

-

A

-

L

-

S

M

H

P

P

A

A

V

V

V

A

L

D

F

I

G

G

E

F

G

T

G

A

G

V

G

H

F

A

A

K

Q

Y

A

Q

S

K

Q

P

T

W

L

L

N

G

E

T

V

V

D

D

P

S

T

S

-

E

L

V

L

Y

S

S

W

W

T

L

E

D

G

S

L

F

T

L

F

L

I

L

G

-

W

M

L

L

S

G

A

G

V

I

T

P

W

W

G

Q

L

-

G

A

Y

Y

F

F

G

Q

Q

-

P

-

H

-

I

-

V

-

R

R

F

V

M

L

A

S

I

S

R

S

T

S

L

A

K

T

D

Y

V

A

P

Q

V

V

A

L

R

S

N

F

I

L

G

A

M

A

S

F

W

G

M

C

L

L

I

V

S

M

L

A

I

I

G

P

A

A

I

I

S

L

L

I

G

G

V

A

F

I

G

G

-

A

-

S

-

T

-

D

-

W

-

N

-

Q

R

T

A

A

Y

Y

A

G

I

L

R

P

N

D

G

P

M

K

D

T

V

T

Q

E

D

E

P

A

E

D

T

M

I

I

F

L

I

P

I

I

L

V

S

L

D

Q

M

Y

L

L

F

C

H

P

P

V

L

Y

I

I

T

S

G

F

F

F

L

G

Y

L

A

G

A

A

L

V

L

S

A

A

V

V

M

M

S
|
S

T

S

I

A

S
x
D

S
|
S

Q

S

L

I

L

L

V

S

S

A

S

S

S

M

L

F

T

A

E

R

D

N

F

I

Y

Y

R

Q

L

L

F

S

L

F

R

R

K

Q

E

N

A

A

S

S

D

D

K

K

E

E

C

I

V

V

N

W

I

V

G

M

R

R

V

I

C

T

V

V

V

F

L

V

V

F

G

G

L

A

V

S

A

A

A

T

V

A

I

M

A

A

binding 4-methoxy-3-[(1-methylpiperidin-4-yl)oxy]-N-{[3-(propan-2-yl)-1,2-oxazol-5-yl]methyl}benzamide: G63 (≠ W43), Y88 (≠ I62)
binding sodium ion: D185 (= D179), S343 (= S330), D346 (≠ S333), S347 (= S334)

8zqoA Human high-affinity choline transporter cht1 bound to hc-3 under nacl condition, with sodium and chloride ions coordinated. (see paper)
24% identity, 79% coverage: 1:380/481 of query aligns to 16:394/515 of 8zqoA

query
sites
8zqoA

M

I

I

L

A

L

I

V

G

G

V

I

F

W

A

A

M

A

R

W

R

R

A

T

T

K

S

N

S

S

-

G

-

S

-

A

-

E

-

R

S

S

E

E

D

A

Y

I

M

I

L

V

G

G

R

R

A

D

L

I

S

G

P

L

K

L

V

V

A

G

L

F

S

T

A

M

G

T

A
|
A

S
x
T

D

W

M
x
V

S
x
G

G

G

W
x
G

L

Y

L

I

L

N

G

G

L

T

P

A

R

E

A

A

L

V

F

Y

A

V

S

P

G

G

L

Y

G

G

S

L

A

A

W

W

-

A

-

Q

-

A

-

P

-

I

-

G

I
x
Y

G

S

I

L

G

S

L

L

L

I

V

L

G

G

A

G

F

L

F

F

N

-

W

-

T

-

L

-

V

F

A

A

P

K

R

P

L

M

R

R

E

S

Q

K

T

-

V

-

H

-

Y

-

G

-

N

G

A

Y

I

V

T

T

I

M

P

L

S

D

F

P

L

F

A

Q

N

Q

R

I

F

Y

P

G

T

K

Q

R

A

M

L

G

S

G

L

L

R

L

T

F

V

I

S

P

A

A

I

L

V

M

I

G

V

E

I

M

F

F

F
x
W

A

A

V

A

Y

A

T

I

A

F

S

S

G

A

L

L

V

G

A

A

G

-

K

-

L

-

F

-

E

-

S

-

A

T

F

I

S

S

G

V

I

I

F

I

N

D

F

V

G

-

L

-

S

-

D

D

Y

M

A

H

V

I

G

S

I

V

V

I

I

I

T

S

L

A

G

L

V

I

V

A

L

T

A

L

Y

Y

T

T

V

L

V

V

G

G

F

L

L

Y

A

S

V

V

S

A

L

Y

T

T

D
|
D

F

V

V

V

Q

Q

G

L

C

F

I

C

M

I

M

F

L

V

A

G

L

L

V

W

I

I

-

S

-

V

-

P

-

F

-

A

-

L

-

S

M

H

P

P

A

A

V

V

V

A

L

D

F

I

G

G

E

F

G

T

G

A

G

V

G

H

F

A

A

K

Q

Y

A

Q

S

K

Q

P

T

W

L

L

N

G

E

T

V

V

D

D

P

S

T

S

-

E

L

V

L

Y

S

S

W

W

T

L

E

D

G

S

L

F

T

L

F

L

I

L

G

-

W

M

L

L

S

G

A

G

V

I

T

P

W
|
W

G

Q

L

-

G

A

Y

Y

F

F

G

Q

Q

-

P

-

H

-

I

-

V

-

R

R

F

V

M

L

A

S

I

S

R

S

T

S

L

A

K

T

D

Y

V

A

P

Q

V

V

A

L

R

S

N

F

I

L

G

A

M

A

S

F

W

G

M

C

L

L

I

V

S

M

L

A

I

I

G

P

A

A

I

I

S

L

L

I

G

G

V

A

F

I

G

G

-

A

-

S

-

T

-

D

-

W

-

N

-

Q

R

T

A

A

Y

Y

A

G

I

L

R

P

N

D

G

P

M

K

D

T

V

T

Q

E

D

E

P

A

E

D

T

M

I

I

F

L

I

P

I

I

L

V

S

L

D

Q

M

Y

L

L

F

C

H

P

P

V

L

Y

I

I

T

S

G

F

F

F

L

G

Y

L

A

G

A

A

L

V

L

S

A

A

A
|
A

V

V

M

M

S
|
S

T
x
S

I

A

S
x
D

S
|
S

Q

S

L

I

L

L

V

S

S

A

S

S

S

M

L

F

T

A

E

R

D

N

F

I

Y

Y

R

Q

L

L

F

S

L

F

R

R

K

Q

E

N

A

A

S

S

D

D

K

K

E

E

C

I

V

V

N

W

I

V

G

M

R

R

V

I

C

T

V

V

V

F

L

V

V

F

G

G

L

A

V

S

A

A

A

T

V

A

I

M

A

A

binding chloride ion: G64 (≠ W43), W139 (≠ F121)
binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): Y89 (≠ I62), W252 (= W238)
binding sodium ion: A58 (= A37), T59 (≠ S38), T59 (≠ S38), V61 (≠ M40), G62 (≠ S41), D186 (= D179), A341 (= A327), S344 (= S330), S344 (= S330), S345 (≠ T331), D347 (≠ S333), S348 (= S334)

Sites not aligning to the query:

binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): 404, 405

8j74A Human high-affinity choline transporter cht1 in the hc-3-bound outward-facing open conformation, dimeric state (see paper)
24% identity, 79% coverage: 1:380/481 of query aligns to 16:394/515 of 8j74A

query
sites
8j74A

M

I

I

L

A

L

I

V

G

G

V

I

F

W

A

A

M

A

R

W

R

R

A

T

T

K

S

N

S

S

-

G

-

S

-

A

-

E

-

R

S

S

E

E

D

A

Y

I

M

I

L

V

G

G

R

R

A

D

L

I

S

G

P

L

K

L

V

V

A

G

L

F

S

T

A

M

G

T

A

A

S

T

D
x
W

M

V

S

G

G

G

W

G

L

Y

L

I

L

N

G

G

L

T

P

A

R

E

A

A

L

V

F

Y

A

V

S

P

G

G

L

Y

G

G

S

L

A

A

W

W

-

A

-

Q

-

A

-

P

-

I

-

G

I
x
Y

G

S

I

L

G

S

L

L

L

I

V

L

G

G

A

G

F

L

F

F

N

-

W

-

T

-

L

-

V

F

A

A

P

K

R

P

L

M

R

R

E

S

Q

K

T

-

V

-

H

-

Y

-

G

-

N

G

A

Y

I

V

T

T

I

M

P

L

S

D

F

P

L

F

A

Q

N

Q

R

I

F

Y

P

G

T

K

Q

R

A

M

L

G

S

G

L

L

R

L

T

F

V

I

S

P

A

A

I

L

V

M

I

G

V

E

I

M

F

F

F
x
W

A

A

V

A

Y

A

T

I

A

F

S

S

G

A

L

L

V

G

A

A

G

-

K

-

L

-

F

-

E

-

S

-

A

T

F

I

S

S

G

V

I

I

F

I

N

D

F

V

G

-

L

-

S

-

D

D

Y

M

A

H

V

I

G

S

I

V

V

I

I

I

T

S

L

A

G

L

V

I

V

A

L

T

A

L

Y

Y

T

T

V

L

V

V

G

G

F

L

L

Y

A

S

V

V

S

A

L

Y

T

T

D

D

F

V

V

V

Q

Q

G

L

C

F

I

C

M

I

M

F

L

V

A

G

L

L

V

W

I

I

-

S

-

V

-

P

-
x
F

-

A

-

L

-
x
S

M
x
H

P

P

A

A

V

V

V

A

L

D

F
x
I

G
|
G

E
x
F

G

T

G

A

G

V

G
x
H

F

A

A

K

Q

Y

A

Q

S

K

Q

P

T

W

L

L

N

G

E

T

V

V

D

D

P

S

T

S

-

E

L

V

L

Y

S

S

W

W

T

L

E

D

G

S

L

F

T

L

F

L

I
x
L

G

-

W

M

L

L

S

G

A

G

V

I

T

P

W
|
W

G

Q

L

-

G

A

Y

Y

F

F

G

Q

Q

-

P

-

H

-

I

-

V

-

R

R

F

V

M

L

A

S

I

S

R

S

T

S

L

A

K

T

D

Y

V

A

P

Q

V

V

A

L

R

S

N

F

I

L

G

A

M

A

S

F

W

G

M

C

L

L

I

V

S

M

L

A

I

I

G

P

A

A

I

I

S

L

L

I

G

G

V

A

F

I

G

G

-

A

-

S

-

T

-

D

-

W

-

N

-

Q

R

T

A

A

Y

Y

A

G

I

L

R

P

N

D

G

P

M

K

D

T

V

T

Q

E

D

E

P

A

E

D

T

M

I

I

F

L

I

P

I

I

L

V

S

L

D

Q

M

Y

L

L

F
x
C

H
x
P

P

V

L

Y

I

I

T

S

G

F

F

F

L

G

Y

L

A

G

A

A

L

V

L

S

A

A

V

V

M

M

S

S

T

S

I

A

S

D

S

S

Q

S

L

I

L

L

V

S

S

A

S

S

S

M

L

F

T

A

E

R

D

N

F

I

Y

Y

R

Q

L

L

F

S

L

F

R

R

K

Q

E

N

A

A

S

S

D

D

K

K

E

E

C

I

V

V

N

W

I

V

G

M

R

R

V

I

C

T

V

V

V

F

L

V

V

F

G

G

L

A

V

S

A

A

A

T

V

A

I

M

A

A

binding Lauryl Maltose Neopentyl Glycol: F203 (vs. gap), S206 (vs. gap), H207 (≠ M193), I213 (≠ F199), G214 (= G200), F215 (≠ E201), H219 (≠ G205), C326 (≠ F312), P327 (≠ H313)
binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): W60 (≠ D39), Y89 (≠ I62), W139 (≠ F121), L245 (≠ I230), W252 (= W238)

Sites not aligning to the query:

binding (2S,2'S)-2,2'-biphenyl-4,4'-diylbis(2-hydroxy-4,4-dimethylmorpholin-4-ium): 404, 405, 408

Q9GZV3 High affinity choline transporter 1; hCHT1; Hemicholinium-3-sensitive choline transporter; CHT; Solute carrier family 5 member 7 from Homo sapiens (Human) (see 5 papers)
24% identity, 79% coverage: 1:380/481 of query aligns to 18:396/580 of Q9GZV3

query
sites
Q9GZV3

M

I

I

L

A

L

I

V

G

G

V

I

F

W

A

A

M

A

R

W

R

R

A

T

T

K

S

N

S

S

-

G

-

S

-

A

-

E

-

R

S

S

E

E

D

A

Y

I

M

I

L

V

G

G

R

R

A
x
D

L

I

S

G

P

L

K

L

V

V

A

G

L

F

S

T

A

M

G

T

A

A

S

T

D

W

M

V

S

G

G
|
G

W

G

L

Y

L

I

L

N

G

G

L

T

P

A

R

E

A

A

L

V

F

Y

A

V

S

P

G

G

L

Y

G

G

S

L

A

A

W

W

-

A

-

Q

-

A

-

P

-
x
I

-

G

I

Y

G

S

I

L

G

S

L

L

L

I

V

L

G

G

A

G

F

L

F

F

N

-

W

-

T

-

L

-

V

F

A

A

P

K

R
x
P

L

M

R

R

E

S

Q

K

T

-

V

-

H

-

Y

-

G

-

N

G

A
x
Y

I

V

T

T

I

M

P

L

S

D

F

P

L

F

A

Q

N

Q

R

I

F

Y

P

G

T

K

Q

R

A

M

L

G

S

G

L

L

R

L

T

F

V

I

S

P

A

A

I

L

V

M

I

G

V

E

I

M

F

F

F

W

A

A

V

A

Y

A

T

I

A

F

S

S

G

A

L

L

V

G

A

A

G

-

K

-

L

-

F

-

E

-

S

-

A

T

F

I

S

S

G

V

I

I

F

I

N

D

F

V

G

-

L

-

S

-

D

D

Y

M

A

H

V

I

G

S

I

V

V

I

I

I

T

S

L

A

G

L

V

I

V

A

L

T

A

L

Y
|
Y

T

T

V

L

V

V

G

G

F

L

L

Y

A

S

V

V

S

A

L

Y

T

T

D

D

F

V

V

V

Q

Q

G

L

C

F

I

C

M

I

M

F

L

V

A

G

L

L

V

W

I

I

-

S

-

V

-

P

-

F

-

A

-

L

-

S

M

H

P

P

A

A

V

V

V

A

L

D

F

I

G

G

E

F

G

T

G

A

G

V

G

H

F

A

A

K

Q

Y

A

Q

S

K

Q

P

T

W

L

L

N

G

E

T

V

V

D

D

P

S

T

S

-

E

L

V

L

Y

S

S

W

W

T

L

E

D

G

S

L

F

T

L

F

L

I

L

G

-

W

M

L

L

S

G

A

G

V

I

T

P

W

W

G

Q

L

-

G

A

Y

Y

F

F

G

Q

Q

-

P

-

H

-

I

-

V

-

R

R

F

V

M

L

A

S

I

S

R

S

T

S

L

A

K

T

D

Y

V

A

P

Q

V

V

A

L

R

S

N

F

I

L

G

A

M

A

S

F

W

G

M

C

L

L

I

V

S

M

L

A

I

I

G

P

A

A

I

I

S

L

L
x
I

G

G

V

A

F

I

G

G

-

A

-

S

-

T

-

D

-

W

-

N

-

Q

R

T

A

A

Y

Y

A

G

I

L

R

P

N

D

G

P

M

K

D

T

V

T

Q

E

D

E

P

A

E

D

T

M

I

I

F

L

I

P

I

I

L

V

S

L

D

Q

M

Y

L

L

F

C

H

P

P

V

L

Y

I

I

T

S

G

F

F

F

L

G

Y

L

A

G

A

A

L

V

L

S

A

A

V
|
V

M

M

S

S

T

S

I

A

S

D

S

S

Q

S

L

I

L

L

V

S

S

A

S

S

S

M

L

F

T

A

E
x
R

D

N

F

I

Y

Y

R

Q

L

L

F

S

L

F

R

R

K

Q

E

N

A

A

S

S

D

D

K

K

E

E

C

I

V

V

N

W

I

V

G

M

R

R

V

I

C

T

V

V

V

F

L

V

V

F

G

G

L

A

V

S

A

A

A

T

V

A

I

M

A

A

D48 (≠ A25) to G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039768
G65 (= G42) to E: in CMS20; loss of choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039765
I89 (vs. gap) to V: 40% reduction in choline transmembrane transporter activity; found in 0.06 of Ashkenazi Jews; dbSNP:rs1013940; mutation to A: Decreased choline transmembrane transporter activity, only 20% of wild-type choline uptake activity.
P105 (≠ R80) to S: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs886039766
Y111 (≠ A91) to H: in CMS20; no effect on localization at plasma membrane
Y175 (= Y166) to C: in CMS20; uncertain significance; dbSNP:rs1331713195
I291 (≠ L282) to T: in CMS20; uncertain significance; dbSNP:rs375397889
V344 (= V328) to L: in CMS20; uncertain significance
R361 (≠ E345) to Q: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane; dbSNP:rs147656110

Sites not aligning to the query:

418 F → V: in CMS20; uncertain significance
446 R → G: in CMS20; decreased choline transmembrane transporter activity; no effect on localization at plasma membrane
451 E→Q: Decreased choline transmembrane transporter activity, only 5% of wild-type choline uptake activity.
527:532 Dileucine-like motif
530 I→A: No change in protein internalization. No change in choline transmembrane transporter activity.
531 L→A: Loss of protein internalization to vesicular structures in neurons. Increased choline transmembrane transporter activity.
531:532 LV→AA: Decreased protein internalization; when associated with V-538. Increased choline transmembrane transporter activity; when associated with V-538.
532 V→A: Decreased protein internalization. Increased choline transmembrane transporter activity.
538 K→V: Decreased protein internalization; when associated with 531-L-V-532. Increased choline transmembrane transporter activity; when associated with 531-L-V-532.

7slaA Cryoem structure of sglt1 at 3.15 angstrom resolution (see paper)
24% identity, 77% coverage: 51:420/481 of query aligns to 42:454/585 of 7slaA

query
sites
7slaA

A

S

L

L

F

F

A

A

S

S

G

N

L

I

G

G

S

S

-

G

A

H

W

F

I

I

G

G

I

L

-

A

-

G

-

T

-

G

-

A

-

S

G

G

L

L

L

A

V

V

G

G

A

G

F

F

-

E

-

W

-

N

-

A

-

L

-

V

-

L

-

V

F

L

N

G

W

W

T

V

L

F

V

V

A

P

P

I

R

Y

L

I

R

K

E

-

Q

-

T

-

V

-

H

-

Y

-

G

A

N

G

A

V

I

V

T

T

I

M

P

P

S

E

F

Y

L

L

A

R

N

K

R

R

F

F

P

G

T

G

Q

Q

A

R

L

I

S

Q

L

-

R

-

T

-

V

V

S

Y

A

L

I

S

V

V

I

L

V

S

I

L

F

F

F

L

A

Y

V

I

Y

F

T

T

A

-

S

-

G

-

L

-

V

-

A

-

G

-

K

K

L

I

F

S

E

V

S

D

A

I

F

F

S

S

G

G

-

A

I

I

F

F

-

I

N

N

F

L

G

A

G

L

L

G

S

W

D

N

Y

L

A

Y

V

L

G

S

I

I

V

I

I

L

T

L

L

L

G

A

V

I

V

T

L

A

A

L

Y

Y

T

T

V

I

V

T

G

G

F

L

L

A

A

A

V

V

S

I

L

Y

T

T

D

D

F

T

V

L

Q

Q

G

T

C

L

I

I

M

M

M

L

L

I

A

G

L

A

V

L

I

I

M

L

P

M

A

G

V

F

V

A

L

-

F

F

G

H

E

E

G

V

G

G

G

Y

F

D

A

A

Q

F

A

M

S

E

Q

K

T

Y

L

M

N

K

E

A

V

I

D

-

P

P

T

T

L

I

L

V

S

S

-

D

-

G

-

N

-

T

-

F

-

Q

-

E

-

K

-

C

-

Y

-

T

-

P

-

R

-

A

-

D

-

S

-

F

-

H

-

I

-

F

-

R

-

D

-

P

W

L

T

T

E

G

G

D

L

L

T

P

F

W

I

P

G

G

W

F

L

I

S

F

A

G

V

L

T

T

-

I

W

L

G

A

L

L

G

W

Y

Y

F

W

G

C

Q

T

P

D

H

Q

I

V

I

I

V

V

-

Q

R

R

F

C

M

L

A

A

I

A

R

K

T

N

L

M

K

S

D

H

V

V

P

K

-

G

-

C

-

I

V

L

A

A

R

G

N
x
Y

I

L

G

K

M

L

S

L

W

P

M

M

L

F

I

I

S

M

L

V

I

M

-

P

G

G

A

M

I

I

S

S

L

R

G

I

V

L

F

F

G

P

R

D

A

K

Y

V

A

A

I

C

R

V

N

V

G

P

M

S

D

E

V

C

Q

E

D

K

P

Y

E

C

T

G

I

T

F

K

I

V

I

G

L

C

S

T

D

N

M

I

L

A

F

Y

H

P

P

T

L

L

I

V

T

V

-

E

-

L

-

M

-

P

-

N

-

G

-

L

-

R

G

G

F

L

L

M

Y

L

A

A

A

V

L

M

L

L

A

A

V

L

M

M

S

S

T

S

I

L

S

T

S

S

Q

I

L

F

L

N

V

S

S

A

S

S

S

T

S

L

L

F

T

T

E

M

D

D

F

I

Y

Y

R

A

L

K

F

-

L

V

R

R

K

K

E

R

A

A

S

S

D

E

K

K

E

E

C

L

V

M

N

I

I

V

G

G

R

R

V

L

C

F

V

V

V

L

L

F

V

L

G

V

L

V

V

V

A

S

-

I

-

A

-

W

-

I

A

P

V

I

I

V

A

Q

S

S

D

A

P

Q

N

S

S

G

Q

Q

V

L

L

F

A

D

L

Y

V

I

A

Q

N

S

A

V

W

S

A

S

G

Y

F

L

G

A

A

P

F

V

G

A

P

A

L

V

I

F

I

L

L

L

S

A

L

I

M

F

W

W

P

K

R

R

T

V

N

N

G

E

A

Q

G

G

A

A

binding cholesterol hemisuccinate: Y288 (≠ N266)

Sites not aligning to the query:

binding cholesterol hemisuccinate: 36, 554

7sl8A Cryoem structure of sglt1 at 3.4 a resolution (see paper)
24% identity, 77% coverage: 51:420/481 of query aligns to 41:453/582 of 7sl8A

query
sites
7sl8A

A

S

L

L

F

F

A

A

S

S

G

N

L

I

G

G

S

S

-

G

A

H

W

F

I

I

G

G

I

L

-

A

-

G

-

T

-

G

-

A

-

S

G

G

L

L

L

A

V

V

G

G

A

G

F

F

-

E

-

W

-

N

-

A

-

L

-

V

-

L

-

V

F

L

N

G

W

W

T

V

L

F

V

V

A

P

P

I

R

Y

L

I

R

K

E

-

Q

-

T

-

V

-

H

-

Y

-

G

A

N

G

A

V

I

V

T

T

I

M

P

P

S

E

F

Y

L

L

A

R

N

K

R

R

F

F

P

G

T

G

Q

Q

A

R

L

I

S

Q

L

-

R

-

T

-

V

V

S

Y

A

L

I

S

V

V

I

L

V

S

I

L

F

F

F

L

A

Y

V

I

Y

F

T

T

A

-

S

-

G

-

L

-

V

-

A

-

G

-

K

K

L

I

F

S

E

V

S

D

A

I

F

F

S

S

G

G

-

A

I

I

F

F

-

I

N

N

F

L

G

A

G

L

L

G

S

W

D

N

Y

L

A

Y

V

L

G

S

I

I

V

I

I

L

T

L

L

L

G

A

V

I

V

T

L

A

A

L

Y

Y

T

T

V

I

V

T

G

G

F

L

L

A

A

A

V

V

S

I

L

Y

T

T

D

D

F

T

V

L

Q

Q

G

T

C

L

I

I

M

M

M

L

L

I

A

G

L

A

V

L

I

I

M

L

P

M

A

G

V

F

V

A

L

-

F

F

G

H

E

E

G

V

G

G

G

Y

F

D

A

A

Q

F

A

M

S

E

Q

K

T

Y

L

M

N

K

E

A

V

I

D

-

P

P

T

T

L

I

L

V

S

S

-

D

-

G

-

N

-

T

-

F

-

Q

-

E

-

K

-

C

-

Y

-

T

-

P

-

R

-

A

-

D

-

S

-

F

-

H

-

I

-

F

-

R

-

D

-

P

W

L

T

T

E

G

G

D

L

L

T

P

F

W

I

P

G

G

W

F

L

I

S

F

A

G

V

L

T

T

-

I

W

L

G

A

L

L

G

W

Y

Y

F

W

G

C

Q

T

P

D

H

Q

I

V

I

I

V

V

-

Q

R

R

F

C

M

L

A

A

I

A

R

K

T

N

L

M

K

S

D

H

V

V

P

K

-
x
G

-

G

-

C

-

I

V

L

A

A

R

G

N
x
Y

I

L

G

K

M

L

S

L

W

P

M

M

L

F

I

I

S

M

L

V

I

M

-

P

G

G

A

M

I

I

S

S

L

R

G

I

V

L

F

F

G

P

R

D

A

K

Y

V

A

A

I

C

R

V

N

V

G

P

M

S

D

E

V

C

Q

E

D

K

P

Y

E

C

T

G

I

T

F

K

I

V

I

G

L

C

S

T

D

N

M

I

L

A

F

Y

H

P

P

T

L

L

I

V

T

V

-

E

-

L

-

M

-

P

-

N

-

G

-

L

-

R

G

G

F

L

L

M

Y

L

A

A

A

V

L

M

L

L

A

A

V

L

M

M

S

S

T

S

I

L

S

T

S

S

Q

I

L

F

L

N

V

S

S

A

S

S

S

T

S

L

L

F

T

T

E

M

D

D

F

I

Y

Y

R

A

L

K

F

-

L

V

R

R

K

K

E

R

A

A

S

S

D

E

K

K

E

E

C

L

V

M

N

I

I

V

G

G

R

R

V

L

C

F

V

V

V

L

L

F

V

L

G

V

L

V

V

V

A

S

-

I

-

A

-

W

-

I

A

P

V

I

I

V

A

Q

S

S

D

A

P

Q

N

S

S

G

Q

Q

V

L

L

F

A

D

L

Y

V

I

A

Q

N

S

A

V

W

S

A

S

G

Y

F

L

G

A

A

P

F

V

G

A

P

A

L

V

I

F

I

L

L

L

S

A

L

I

M

F

W

W

P

K

R

R

T

V

N

N

G

E

A

Q

G

G

A

A

binding cholesterol: G280 (vs. gap), Y287 (≠ N266)

Sites not aligning to the query:

binding cholesterol: 35, 551, 558

3dh4A Crystal structure of sodium/sugar symporter with bound galactose from vibrio parahaemolyticus (see paper)
30% identity, 24% coverage: 312:425/481 of query aligns to 310:426/512 of 3dh4A

query
sites
3dh4A

F

F

H

L

P

P

L

V

-

G

I

V

T

K

G

G

F

V

L

V

Y

F

A

A

L

L

L

A

A

A

A
|
A

V

I

M

V

S
|
S

T

S

I

L

S

A

S

S

Q

M

L

L

L

N

V

S

S

T

S

A

S

T

S

I

L

F

T

T

E

M

D

D

F

I

Y

Y

R

K

L

E

F

Y

L

I

R

S

K

P

E

D

A

S

S

G

D

D

K

H

E

K

C

L

V

V

N

N

I

V

G

G

R

R

V

T

C

A

V

A

V

V

L

-

V

-

G

-

L

-

V

V

A

A

A

L

V

I

I

I

A

A

S

-

D

-

P

-

N

-

S

-

Q

-

V

-

L

-

A

C

L

L

V

I

A

A

N

P

A

M

W

L

A

G

G

G

F

I

G

G

A

Q

A

A

F

F

G

Q

-

Y

-

I

-
x
Q

-

E

-

Y

-

T

-

G

-

L

-

V

-

S

-

P

-

G

-

I

-

L

P

A

L

V

I

F

I

L

L

L

S

G

L

L

M

F

W

W

P

K

R

K

T

T

N

T

G

S

A

K

G

G

A

A

I

I

A

I

G

G

M

V

V

V

binding beta-D-galactopyranose: Q393 (vs. gap)
binding sodium ion: A326 (= A327), S329 (= S330)

Sites not aligning to the query:

binding beta-D-galactopyranose: 40, 58, 59, 62, 225, 229, 259
binding sodium ion: 36

Q92911 Sodium/iodide cotransporter; Na(+)/I(-) cotransporter; Natrium iodide transporter; Sodium-iodide symporter; Na(+)/I(-) symporter; Solute carrier family 5 member 5 from Homo sapiens (Human) (see 3 papers)
23% identity, 96% coverage: 10:472/481 of query aligns to 38:495/643 of Q92911

query
sites
Q92911

R

R

R

G

A

G

T

Q

S

R

S

S

S

A

E

E

D

D

Y

F

M

F

L

T

G

G

R

R

A

R

L

L

S

A

P

A

K

L

V

P

A

V

A

G

L

L

S

S

A

L

G

S

A

A

S

S

D

F

M

M

S

S

G

A

W

V

L

Q

L

V

L

L

G

G

L

V

P

P

R

S

A

E

L

A

F

Y

A

R

S

Y

G

G

L

L

G

K

S

F

A

L

W

W

I

M

G

C

I

L

G

G

L

Q

L

L

V

L

G

N

A

S

F

V

F

L

N

T

W
x
A

T

L

L

L

V

F

A

M

P

P

R

-

L

-

R

-

E

-

Q

-

T

-

V

V

H

F

Y

Y

G

R

N

L

A

G

I

L

T

T

-

S

I

T

P

Y

S

E

F

Y

L

L

A

E

N

M

R

R

F

F

P

-

T

-

Q

-

A

S

L

R

S

A

L

V

R

R

T

L

V

C

S

G

A

T

I

L

V

Q

I

Y

V

I

I

V

F

A

F

T

A

M

V

L

Y

Y

T

T

A

G

S

I

G

-

L

V

V

I

A

Y

G

A

G

P

K

A

L

L

F

I

E

L

S

N

A

Q

F

V

S

T

G

G

I

L

F

D

N

I

F

W

G

A

G

S

L

L

S

-

D

-

Y

L

A

S

V

T

G

G

I

I

V

I

I

C

T

T

L

F

G

-

V

-

L

-

A

-

Y

Y

T

T

V

A

V

V

G

G

F

M

L

K

A

A

V

V

S

V

L

W

T

T

D

D

F

V

V

F

Q

Q

G

V

C

V

I

V

M

M

M

L

L

S

A

G

-

F

-

W

L

V

V

V

I

L

M

A

P

R

A

G

V

V

V

M

L

L

F

V

G

G

E

G

G

P

G

R

G

Q

-

V

-

L

G

T

F

L

A

A

Q

Q

A

N

S
x
H

Q

S

T

R

L

I

N

N

E

L

V

M

D

D

-

F

-

N

P

P

T
x
D

L

P

L

R

S

S

W

R

T
x
Y

E
x
T

G

F

L

W

T

T

F

F

I

V

-

V

-

G

-

T

-

L

G

V

W

W

L

L

S

S

A

M

V

-

T

-

W

-

G

-

L

-

G

-

Y

Y

F

G

G

V

Q

N

P

Q

H

A

I

Q

I

V

V

Q

R

R

F

Y

M

V

A

A

I

C

R

R

T

T

L

E

K

K

D

Q

V

A

P

K

V

L

A

A

R

L

N

L

I

I

G

N

M

Q

S

V

W

G

M

L

L

F

I

L

S

I

L

V

I

S

G

S

A

A

I

A

S

C

L

C

G

G

V

I

-

V

-

M

-

F

-

V

-

F

-

Y

-

T

-

D

-

C

-

D

-

P

-

L

F

L

G

G

R

R

A

I

Y

S

A

A

I

P

R

D

N

Q

G

Y

M

M

D

-

V

-

Q

-

D

-

P

P

E

L

T

L

I

V

F

L

I

D

I

I

L

F

S

E

D

D

M

L

L

-

F

-

H

-

P

P

L

G

I

V

T

P

G

G

F

L

L

F

Y

L

A

A

A

C

L

A

L

Y

A

S

A

G

V

T

M

L

S

S

T

T

I

A

S

S

S

T

Q

S

L

I

L

N

V

A

S

M

S

A

S

V

L

T

T

V

E

E

D

D

F

L

Y

I

R

K

L

P

F

R

L

L

R

R

K

S

E

L

A

A

S

P

D

-

K

R

E

K

C

L

V

V

N

I

I

I

G

S

R

K

V

G

C

L

V

S

V

L

L

I

V

Y

G

G

L

S

V

A

A

C

A

L

V

T

I

V

A

A

S

A

D

L

P

S

N

S

S

L

Q

L

V

G

L

G

A

G

L

V

V

L

A

Q

N

G

A

S

W

F

A

T

G

V

F

M

G

G

A

V

A

I

F

S

G

G

P

P

L

L

I

L

-

G

-

A

-

F

I

I

L

L

S

G

L

M

M

F

W

L

P

P

R

A

T

C

N

N

G

T

A

P

G

G

A

-

I

V

A

L

G

A

M

G

V

L

V

G

G

A

A

G

A

L

T

A

V

L

M

S

I

L

W

W

I

V

S

A

L

L

G

G

W

A

N

T

G

-

S

L

F

Y

M

P

G

P

G

S

P

E

G
x
Q

V

T

Y

M

E

R

I

V

I

L

P

P

G

S

F

S

I

A

A

A

A

R

F

C

I

V

A

A

I

L

L

S

V

V

V

N

S

A

S

S

A

G

T

L

A

L

D

D

A102 (≠ W74) natural variant: A -> P
H226 (≠ S210) mutation H->A,D,E,K: Significant loss of iodide transport activity but no effect on its localization to the cell membrane.
D237 (≠ T219) mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization.
Y242 (≠ T224) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471. Loss of iodide transport activity; when associated with F-535.
T243 (≠ E225) Required for homodimerization; mutation to A: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Reduced homodimerization; when associated with A-471.
Q471 (≠ G448) Required for homodimerization; mutation to A: No effect on localization to the cell membrane, iodide transport activity and homodimerization. Significant loss of homodimerization; when associated with A-242 or A243.

Sites not aligning to the query:

525 A→F: Loss of localization to the cell membrane, significant loss of iodide transport activity but no effect on homodimerization. Loss of iodide transport activity; when associated with A-242.
536 T → Q: requires 2 nucleotide substitutions
556 S → Q: requires 2 nucleotide substitutions

8j77A Human high-affinity choline transporter cht1 in the choline-bound inward-facing occluded conformation (see paper)
27% identity, 48% coverage: 151:380/481 of query aligns to 140:372/493 of 8j77A

query
sites
8j77A

D

D

Y

M

A

H

V

I

G

S

I

V

V

I

I

I

T

S

L

A

G

L

V

I

V

A

L

T

A

L

Y

Y

T

T

V

L

V

V

G

G

F

L

L

Y

A

-

V

-

S

-

L

-

T

T

D

D

F

V

V

V

Q

Q

G

L

C

F

I

C

M

I

M

F

L

V

A

G

L

L

V

W

I

I

-

S

-

V

-

P

-

F

-

A

-

L

-

S

M

H

P

P

A

A

V

V

V

A

L

D

F

I

G

G

E

F

G

T

G

A

G

V

G

H

F

A

A

K

Q

Y

A

Q

S

K

Q

P

T

W

L

L

N

G

E

T

V

V

D

D

P

S

T

S

-

E

L

V

L

Y

S

S

W

W

T

L

E

D

G

S

L

F

T

L

F

L

I

L

G

-

W

M

L

L

S

G

A

G

V

I

T

P

W
|
W

G

Q

L

-

G

A

Y

Y

F

F

G

Q

Q

-

P

-

H

-

I

-

V

-

R

R

F

V

M

L

A

S

I

S

R

S

T

S

L

A

K

T

D

Y

V

A

P

Q

V

V

A

L

R

S

N

F

I

L

G

A

M

A

S

F

W

G

M

C

L

L

I

V

S

M

L

A

I

I

G

P

A

A

I

I

S

L

L

I

G

G

V

A

F

I

G

G

-

A

-

S

-

T

-

D

-

W

-

N

-

Q

R

T

A

A

Y

Y

A

G

I

L

R

P

N

D

G

P

M

K

D

T

V

T

Q

E

D

E

P

A

E

D

T

M

I

I

F

L

I

P

I

I

L

V

S

L

D

Q

M

Y

L

L

F

C

H

P

P

V

L

Y

I

I

T

S

G

F

F

F

L

G

Y

L

A

G

A

A

L

V

L

S

A

A

V

V

M

M

S

S

T

S

I

A

S

D

S

S

Q

S

L

I

L

L

V

S

S

A

S

S

S

M

L

F

T

A

E

R

D

N

F

I

Y

Y

R

Q

L

L

F

S

L

F

R

R

K

Q

E

N

A

A

S

S

D

D

K

K

E

E

C

I

V

V

N

W

I

V

G

M

R

R

V

I

C

T

V

V

V

F

L

V

V

F

G

G

L

A

V

S

A

A

A

T

V

A

I

M

A

A

binding choline ion: W230 (= W238)

Sites not aligning to the query:

binding choline ion: 42, 71

Q9Y289 Sodium-dependent multivitamin transporter; Na(+)-dependent multivitamin transporter; hSMVT; Solute carrier family 5 member 6 from Homo sapiens (Human) (see 10 papers)
26% identity, 91% coverage: 3:438/481 of query aligns to 41:473/635 of Q9Y289

query
sites
Q9Y289

A

A

I

I

G

G

V

L

F

Y

A

H

M

A

R

C

R

R

A

G

-

W

-

G

T

R

S

H

S

T

S

V

E

G

D

E

Y

L

M

L

L

M

G

A

G

D

R

R

A

K

L

M

S

G

P
x
C

K

L

V

P

A

V

A

A

L

L

S

S

A

L

G

L

A

A

S
x
T

D

F

M

Q

S

S

G

A

W

V

L

A

L

I

L

L

G

G

L

V

P

P

R

S

A

E

L

I

F

Y

A
x
R

S
x
F

G
|
G

L
x
T

G
x
Q

S
x
Y

A
x
W

W
x
F

I
x
L

G
|
G

I
x
C

G
x
C

L
x
Y

L
x
F

V
x
L

G
|
G

A
x
L

F
x
L

F
x
I

N
x
P

W
x
A

T
x
H

L
x
I

V
x
F

A
x
I

P
|
P

R
x
V

L
x
F

R
x
Y

E
x
R

Q

-

T

-

V
x
L

H
|
H

Y
x
L

G
x
T

N
x
S

A
|
A

I
x
Y

T

-

I

-

P

-

S
x
E

F
x
Y

L
|
L

A
x
E

N
x
L

R
|
R

F
|
F

P
x
N

T
x
K

Q

-

A

-

L

-

S
x
T

L
x
V

R
|
R

T
x
V

V
x
C

S
x
G

A
x
T

I
x
V

V
x
T

I
x
F

V
x
I

I
x
F

F
x
Q

F
x
M

A
x
V

V
x
I

Y
|
Y

T
x
M

A
x
G

S
x
V

G
x
V

L
|
L

V
x
Y

A
|
A

G
x
P

G

-

K
x
S

L
|
L

F
x
A

E
x
L

S
x
N

A
|
A

F
x
V

S
x
T

G
|
G

I

-

F
|
F

N
x
D

F
x
L

G
x
W

G

-

L
|
L

S
|
S

D
x
V

Y
x
L

A
|
A

V
x
L

G
|
G

I
|
I

V
|
V

I
x
C

T
|
T

L
x
V

G

-

V

-

L

-

A

-

Y
|
Y

T
|
T

V
x
A

V
x
L

G
|
G

F
x
L

L
x
K

A
|
A

V
|
V

S
x
I

L
x
W

T
|
T

D
|
D

F
x
V

V
x
F

Q
|
Q

G
x
T

C
x
L

I
x
V

M
|
M

M
x
F

L
|
L

A
x
G

L
x
Q

V
x
L

I

-

M

-

P

-

A
|
A

V
|
V

V
x
I

L
x
I

F
x
V

G
|
G

E
x
S

-
x
A

-
x
K

-
x
V

G
|
G

G
x
L

G
|
G

-
x
R

-
x
V

F
x
W

A
|
A

Q
x
V

A
|
A

S
|
S

Q
|
Q

T
x
H

L
x
G

N
x
R

-
x
I

-
x
S

-
x
G

-
x
F

E
|
E

V
x
L

D
|
D

P
|
P

T
x
D

L
x
P

L
x
F

S
x
V

-
x
R

-
x
H

-
x
T

-
x
F

W
|
W

T
|
T

E

-

G

-

L
|
L

T
x
A

F
|
F

I
x
G

G
|
G

W

-

L

-

S

-

A

-

V
|
V

T
x
F

W
x
M

G
x
M

L
|
L

G
x
S

Y
x
L

F
x
Y

G
|
G

Q
x
V

P
x
N

H
x
Q

I
x
A

I
x
Q

V
|
V

-
x
Q

R
|
R

F
x
Y

M
x
L

A
x
S

I
x
S

R
|
R

T
|
T

L
x
E

K
|
K

D
x
A

V
x
A

P
x
V

V
x
L

A
x
S

R
x
C

N
x
Y

I
x
A

G
x
V

M
x
F

S
x
P

W
x
F

M
x
Q

L
x
Q

I
x
V

S
|
S

L
|
L

I
x
C

G
x
V

A
x
G

I
x
C

S
x
L

L
x
I

G
|
G

V
x
L

F
x
V

G
x
M

R
x
F

A
|
A

Y
|
Y

A
x
Y

I
x
Q

R
x
E

N
x
Y

G
x
P

M
|
M

D
x
S

V
x
I

Q
|
Q

-
x
Q

-
x
A

-
x
Q

-
x
A

-
x
P

D
|
D

P
x
Q

E
x
F

T
x
V

I
x
L

F
x
Y

I
x
F

I
x
V

L
x
M

S
x
D

D
x
L

M
x
L

L
x
K

F
x
G

H
x
L

P
|
P

L
x
G

I
x
L

T
x
P

G
|
G

F
x
L

L
x
F

Y
x
I

A
|
A

A
x
C

L
|
L

L
x
F

A
x
S

A
x
G

V
x
S

M
x
L

S
|
S

T
|
T

I
|
I

S
|
S

Q
x
A

L
x
F

L
x
N

V
x
S

S
x
L

S
x
A

S
x
T

S
x
V

L
x
T

T
x
M

E
|
E

D
|
D

F
x
L

Y
x
I

R
|
R

L
x
P

F
x
W

L
x
F

R

-

K
x
P

E
|
E

A
x
F

S
|
S

D
x
E

K
x
A

E
x
R

C
x
A

V
x
I

N
x
M

I
x
L

G
x
S

R
|
R

V
x
G

C
x
L

V
x
A

V
x
F

L
x
G

V
x
Y

G
|
G

L
|
L

V
x
L

A
x
C

A
x
L

V
x
G

I
x
M

A
|
A

S
x
Y

D
x
I

P

-

N
x
S

S
|
S

Q
|
Q

V
x
M

L
x
G

A
x
P

L
x
V

V
x
L

A
x
Q

N
x
A

A
|
A

W
x
I

A
x
S

G
x
I

F
|
F

G
|
G

A
x
M

A
x
V

F
x
G

G
|
G

P
|
P

L
|
L

I
x
L

-
x
G

-
x
L

-
x
F

I
x
C

L
|
L

S
x
G

L
x
M

M
x
F

W
x
F

P
|
P

R
x
C

T
x
A

N
|
N

G
x
P

A
x
P

G
|
G

A
|
A

I
x
V

A
x
V

G
|
G

M
x
L

V
x
L

V
x
A

G
|
G

A

-

A
x
L

T
x
V

V
x
M

M
x
A

I
x
F

W
|
W

I
|
I

S
x
G

L
x
I

G
|
G

C68 (≠ P28) mutation to A: No effect on biotin transport.
T78 (≠ S38) mutation to A: Reduced membrane localization. Decrease in biotin transport.
C104 (≠ I64) mutation to A: No effect on biotin transport.
R123 (≠ E83) to L: in SMVTD; reduced membrane localization; impaired biotin transport
S128 (≠ N90) mutation to A: No effect on biotin transport.
N138 (≠ P103) mutation to A: Reduced protein levels. Decrease in biotin transport.
C144 (≠ V112) mutation to A: No effect on biotin transport.
Y162 (≠ V130) to C: in COMNB; no effect on membrane localization
C187 (≠ I158) mutation to A: No effect on biotin transport.
S242 (vs. gap) mutation to A: No effect on biotin transport.
S283 (≠ A254) mutation to A: No effect on protein levels or membrane localization.
T286 (= T257) mutation to A: Resistant to phorbol 12-myristate 13-acetate (PMA)-induced inhibition of biotin transport. No effect on protein levels or membrane localization.
C294 (≠ R265) mutation to A: Reduced membrane localization. Decrease in biotin transport (decreased Vmax, no change in Km).; mutation C->S,M: Decrease in biotin transport.
C309 (≠ I280) mutation to A: No effect on biotin transport.
C358 (≠ A323) mutation to A: No effect on biotin transport.
T366 (= T331) mutation to A: No effect on biotin transport.
R400 (= R366) to T: in SMVTD; impaired biotin transport; dbSNP:rs370950187
C410 (≠ A376) mutation to A: No effect on biotin transport.
S429 (≠ A396) to G: in COMNB; no effect on membrane localization
C443 (≠ I407) mutation to A: No effect on biotin transport.
C450 (≠ R414) mutation to A: No effect on biotin transport.

Sites not aligning to the query:

94:635 natural variant: Missing (in SMVTD and COMNB; reduced membrane localization; impaired biotin transport; dbSNP:rs994218778)
481 S → F: in dbSNP:rs1395
489 N→A: Slight decrease in protein levels. Decrease in biotin transport.
498 N→A: No effect on biotin transport.
534 N→A: No effect on biotin transport.
567:635 mutation Missing: Loss of biotin transport. Loss of membrane localization.
570:635 mutation Missing: Loss of biotin transport. Loss of membrane localization.
575:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
577 C→A: No effect on biotin transport.
583 C→A: No effect on biotin transport.
584:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
600:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
612:635 mutation Missing: Partial loss (75%) of biotine transport. Apical membrane localization and intracellular structure localization in polarized cells.
616:635 mutation Missing: Loss of apical membrane localization in polarized cells. Basolateral localization in polarized cells.
620:635 mutation Missing: Partial loss (25%) of biotine transport. No change in apical membrane localization in polarized cells.
624:635 mutation Missing: Partial loss (25%) of biotine transport. No change in apical membrane localization in polarized cells.
627 T→A: No effect on biotin transport.
628 mutation C->A,S: Decrease in biotin transport.
632:635 mutation Missing: Partial loss (25%) of biotine transport. No change in apical membrane localization in polarized cells.

Q8N695 Sodium-coupled monocarboxylate transporter 1; Apical iodide transporter; Electrogenic sodium monocarboxylate cotransporter; Sodium iodide-related cotransporter; Solute carrier family 5 member 8 from Homo sapiens (Human) (see 3 papers)
22% identity, 88% coverage: 14:438/481 of query aligns to 40:459/610 of Q8N695

query
sites
Q8N695

S

Q

S

T

S

S

E

K

D

D

Y

F

M

L

L

M

G

G

R

R

A

R

L

M

S

T

P

A

K

V

V

P

A

V

A

A

L

L

S

S

A

L

G

T

A

A

S

S

D

F

M

M

S

S

G

A

W

V

L

T

L

V

L

L

G

G

L

T

P

P

R

S

A

E

L

V

F

Y

A

R

S

F

G

G

L

-

G

-

S

-

A

-

W

-

I

A

G

I

I

F

G

S

L

I

L

F

V

A

G

F

A

T

F

Y

F

F

N

F

W

V

T

V

L

V

V

I

A

S

P

A

R

E

L

V

R

F

E

L

Q

P

T

V

V

F

H

Y

Y

K

G

L

N

G

A

I

I

T

T

S

I

T

P

Y

S

E

F

Y

L

L

A

E

N

L

R

R

F

F

P

N

T

K

Q

-

A

-

L

-

S

C

L

V

R

R

T

L

V

C

S

G

A

T

I

V

V

L

I

F

V

I

I

V

F

Q

F

T

A

I

V

L

Y

Y

T

T

A

G

S

I

G

-

L

V

V

I

A

Y

G

A

G

P

K

A

L

L

F

A

E

L

S

N

A

Q

F

V

S

T

G

G

I

F

F

D

N

L

F

W

G

G

G

A

L

V

S

V

D

-

Y

-

A

A

V

T

G

G

I

V

V

V

I

C

T

T

L

F

G

-

V

-

L

-

A

-

Y

Y

T

C

V

T

V

L

G

G

F

L

L

K

A

A

V

V

S

I

L

W

T

T

D

D

F

V

V

F

Q

Q

G
x
V

C

G

I

I

M

M

M

V

-

A

-

G

L

F

A

A

L

S

V

V

I

I

M

I

P

Q

A

A

V

V

L

M

F

-

G

-

E

Q

G

G

G

-

F

-

A

-

Q

-

A

I

S

S

Q

T

T

I

L

L

N

N

E

D

V

A

-

Y

-

D

-

G

-

R

-

L

-

N

-

F

-

W

-

N

-

F

D

N

P

P

T

N

L

P

L

L

S

Q

-

R

-

H

-

T

-

F

W

W

T

T

E

-

G

-

L

-

T

I

F

I

I

I

G

G

W

-

L

-

S

G

A

T

V
x
F

T

T

W

W

G

T

L

S

G

I

Y

Y

F

G

G

V

Q

N

P

Q

H

S

I

Q

I

V

V

Q

R

R

F

Y

M

I

A

S

I

C

R

K

T

S

L

R

K

F

D

Q

V

A

P

K

V

L

A

S

R

L

N

Y

I

I

G

N

M

L

S

V

-

G

-

L

W

W

M

A

L

I

I

L

S

T

L

C

I

S

G

V

A

F

I

C

S

G

L

L

G

A

V

L

F

Y

G

S

R

R

A

Y

Y

H

A

D

I

C

R

D

-

P

-

W

N

T

G

A

M

K

D

K

V

V

Q

S

D

A

P

P

E

D

T

Q

I

L

-

M

-

P

F

Y

I

L

I

V

L

L

S

D

D

I

M

L

L

Q

F

D

H

Y

P

P

L

G

I

L

T

P

G

G

F

L

L

F

Y

V

A

A

A

C

L

A

L

Y

A

S

A

G

V

T

M

L

S

S

T

T

I

V

S

S

Q

S

L

I

L

N

V

A

S

L

S

A

S

V

L

T

T

V

E

E

D

D

F

L

Y

I

R

K

L

P

F

Y

L

F

R

R

K

S

E

L

A

S

-

E

-

R

-

S

-

L

-

S

-

W

-

I

S

S

D

Q

K

G

E

M

C

S

V

V

N

V

I

Y

G

G

R

A

V

L

C

C

V

I

V

G

L

M

V

A

G

A

L

L

V

A

A

-

V

-

I

-

A

-

S

-

D

-

P

-

N

-

S

S

Q

L

V

M

L

G

A

A

L

L

V

L

A

Q

N

A

A

A

W

L

A

S

G

V

F

F

G

G

A

M

A

V

F

G

G

G

P

P

L

L

I

M

-

G

-

L

-

F

I

A

L

L

S

G

L

I

M

L

W

V

P

P

R

F

T

A

N

N

G

S

A

I

G

G

A

A

I

L

A

V

G

G

M

L

V

M

V

A

G

G

A

F

A

A

T

-

V

I

M

S

I

L

W

W

I

V

S

G

L

I

G

G

V193 (≠ G183) to I: in dbSNP:rs1709189
F251 (≠ V236) to V: in dbSNP:rs11834933

Sites not aligning to the query:

608 T→A: Loss of interaction with PDZK1.
608:610 PDZ-binding
610 L→A: Loss of interaction with PDZK1.

7wmvA Structure of human sglt1-map17 complex bound with lx2761 (see paper)
28% identity, 22% coverage: 316:420/481 of query aligns to 361:468/602 of 7wmvA

query
sites
7wmvA

I

L

T

R

G

G

F

L

L

M

Y

L

A

S

A

V

L

M

L

L

A

A

A

S

V

L

M

M

S

S

T

S

I

L

S

T

S

S

Q

I

L

F

L

N

V

S

S

A

S

S

S

T

S

L

L

F

T

T

E

M

D

D

F

I

Y

Y

R

A

L

K

F

-

L

V

R

R

K

K

E

R

A

A

S

S

D

E

K

K

E

E

C

L

V

M

N

I

I

A

G

G

R

R

V

L

C

F

V

I

-

L

V

V

L

L

V

I

G

G

L

I

V

S

A

I

A

A

-

W

-

V

-

P

V

I

I

V

A

Q

S

S

D

A

P

Q

N

S

S

G

Q

Q

V

L

L
x
F

A
x
D

L

Y

V

I

A
x
Q

N

S

A

I

W

T

A

S

G

Y

F

L

G

G

A

P

F

I

G

A

P

A

L

V

I

F

I

L

L

L

S

A

L

I

M

F

W

W

P

K

R

R

T

V

N

N

G

E

A

P

G

G

A

A

binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: F436 (≠ L388), D437 (≠ A389), Q440 (≠ A392)

Sites not aligning to the query:

binding N-[2-(dimethylamino)ethyl]-2-methyl-2-[4-[4-[[2-methyl-5-[(2S,3R,4R,5S,6R)-6-methylsulfanyl-3,4,5-tris(oxidanyl)oxan-2-yl]phenyl]methyl]phenyl]butanoylamino]propanamide: 61, 66, 70, 81, 84, 257, 266, 269, 270, 273, 274, 508

7yniA Structure of human sglt1-map17 complex bound with substrate 4d4fdg in the occluded conformation (see paper)
28% identity, 22% coverage: 316:420/481 of query aligns to 340:447/566 of 7yniA

query
sites
7yniA

I

L

T

R

G

G

F

L

L

M

Y

L

A

S

A

V

L

M

L

L

A

A

A

S

V

L

M

M

S

S

T

S

I

L

S

T

S

S

Q

I

L

F

L

N

V

S

S

A

S

S

S

T

S

L

L

F

T

T

E

M

D

D

F

I

Y

Y

R

A

L

K

F

-

L

V

R

R

K

K

E

R

A

A

S

S

D

E

K

K

E

E

C

L

V

M

N

I

I

A

G

G

R

R

V

L

C

F

V

I

-

L

V

V

L

L

V

I

G

G

L

I

V

S

A

I

A

A

-

W

-

V

-

P

V

I

I

V

A

Q

S

S

D

A

P

Q

N

S

S

G

Q

Q

V

L

L
x
F

A

D

L

Y

V

I

A
x
Q

N

S

A

I

W

T

A

S

G

Y

F

L

G

G

A

P

F

I

G

A

P

A

L

V

I

F

I

L

L

L

S

A

L

I

M

F

W

W

P

K

R

R

T

V

N

N

G

E

A

P

G

G

A

A

binding (2R,3R,4R,5S,6R)-5-fluoranyl-6-(hydroxymethyl)oxane-2,3,4-triol: F415 (≠ L388), Q419 (≠ A392)

Sites not aligning to the query:

binding (2R,3R,4R,5S,6R)-5-fluoranyl-6-(hydroxymethyl)oxane-2,3,4-triol: 51, 70, 248, 252
binding : 548, 552, 555, 556, 559, 560, 563, 564

P13866 Sodium/glucose cotransporter 1; Na(+)/glucose cotransporter 1; High affinity sodium-glucose cotransporter; Solute carrier family 5 member 1 from Homo sapiens (Human) (see 7 papers)
28% identity, 22% coverage: 316:420/481 of query aligns to 378:485/664 of P13866

query
sites
P13866

I
x
L

T
x
R

G
|
G

F
x
L

L
x
M

Y
x
L

A
x
S

A
x
V

L
x
M

L
|
L

A
|
A

A
x
S

V
x
L

M
|
M

S
|
S

T
x
S

I
x
L

S
x
T

S
|
S

Q
x
I

L
x
F

L
x
N

V
x
S

S
x
A

S
|
S

S
x
T

S
x
L

L
x
F

T
|
T

E
x
M

D
|
D

F
x
I

Y
|
Y

R
x
A

L
x
K

F

-

L
x
V

R
|
R

K
|
K

E
x
R

A
|
A

S
|
S

D
x
E

K
|
K

E
|
E

C
x
L

V
x
M

N
x
I

I
x
A

G
|
G

R
|
R

V
x
L

C
x
F

V
x
I

-
x
L

V
|
V

L
|
L

V
x
I

G
|
G

L
x
I

V
x
S

A
x
I

A
|
A

-
x
W

-
x
V

-
x
P

V
x
I

I
x
V

A
x
Q

S
|
S

D
x
A

P
x
Q

N
x
S

S
x
G

Q
|
Q

V
x
L

L
x
F

A
x
D

L
x
Y

V
x
I

A
x
Q

N
x
S

A
x
I

W
x
T

A
x
S

G
x
Y

F
x
L

G
|
G

A
x
P

F
x
I

G
x
A

P
x
A

L
x
V

I
x
F

I
x
L

L
|
L

S
x
A

L
x
I

M
x
F

W
|
W

P
x
K

R
|
R

T
x
V

N
|
N

G
x
E

A
x
P

G
|
G

A
|
A

R379 (≠ T317) to Q: in GGM; loss of activity; dbSNP:rs747215838
A388 (= A326) to V: in GGM; loss of activity
S396 (= S334) mutation to A: Loss of activity.
F405 (≠ L343) to S: in GGM; loss of activity
A411 (≠ R349) to T: in GGM; slightly decreased activity; dbSNP:rs17683430
G426 (= G365) to R: in GGM; loss of activity; dbSNP:rs1304151494
Q451 (= Q386) mutation to A: Strong reduction in water permeation.
L452 (≠ V387) mutation to A: Loss of water permeation.
D454 (≠ A389) mutation to A: Has no effect on water permeation.
Q457 (≠ A392) mutation to A: Loss of D-glucose transporter activity.; mutation to C: Strong reduction in D-glucose transporter activity.
T460 (≠ W395) mutation to A: Loss of D-glucose transporter activity.
V470 (≠ L405) to N: in GGM; about 90% reduction in activity; requires 2 nucleotide substitutions

Sites not aligning to the query:

51 N → S: in GGM; slightly decreased activity; dbSNP:rs17683011
67 W→A: Strong reduction in D-glucose transporter activity.
77 S→A: Loss of activity.
83 H→L: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and C-290.; H→Q: Loss of D-glucose transporter activity.
135 R → W: in GGM; loss of activity
159 S → P: in GGM; loss of activity; dbSNP:rs933026071
166 A → T: in GGM; about 90% reduction in activity
191:664 natural variant: Missing (in GGM; loss of activity)
204 D→A: Loss of activity.
248 modified: carbohydrate, N-linked (GlcNAc...) asparagine; N→Q: Loss of N-glycosylation.
255 modified: Disulfide link with 511
276 W → L: in GGM; about 95% reduction in activity
287 T→A: Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with L-83 and C-290.; T→N: Loss of D-glucose transporter activity. Has strict selectivity for D-galactose.; mutation T->S,A: Has normal D-glucose and D-galactose transporter activity.
290 Y→C: Loss of D-galactose transporter activity. Has strict selectivity for D-glucose. Acquires D-mannose, D-fructose and L-sorbose transporter activity; when associated with A-287 and L-83.
291 W→A: Loss of D-glucose transporter activity.
292 C → Y: in GGM; loss of activity; dbSNP:rs765502638; C→A: Has no effect on water permeability.
295 Q → R: in GGM; loss of activity; dbSNP:rs779428134
300 R → S: in GGM; loss of activity
304 A → V: in GGM; impairs trafficking to the plasma membrane
321 K→Q: Acquires D-mannose and D-allose transporter activity comparable to glucose and galactose.
345 modified: Disulfide link with 351
351 modified: Disulfide link with 345
355 modified: Disulfide link with 361
361 modified: Disulfide link with 355
363 N→A: Loss of water permeation.
369 L → S: in GGM; loss of activity
379:664 natural variant: Missing (in GGM; loss of activity)
499 R → H: in GGM; impairs trafficking to the plasma membrane; decreases the sugar affinity; dbSNP:rs927157864
511 modified: Disulfide link with 255
517 modified: Disulfide link with 522
522 modified: Disulfide link with 517
615 H → Q: in GGM; slightly decreased activity; dbSNP:rs33954001
641 W→A: Slightly reduced D-glucose transporter activity.
660:661 HA→WG: Loss of D-glucose transporter activity.

Query Sequence

>GFF2255 FitnessBrowser__Marino:GFF2255
MIAIGVFAMRRATSSSEDYMLGGRALSPKVAALSAGASDMSGWLLLGLPRALFASGLGSA
WIGIGLLVGAFFNWTLVAPRLREQTVHYGNAITIPSFLANRFPTQALSLRTVSAIVIVIF
FAVYTASGLVAGGKLFESAFSGIFNFGGLSDYAVGIVITLGVVLAYTVVGGFLAVSLTDF
VQGCIMMLALVIMPAVVLFGEGGGGFAQASQTLNEVDPTLLSWTEGLTFIGWLSAVTWGL
GYFGQPHIIVRFMAIRTLKDVPVARNIGMSWMLISLIGAISLGVFGRAYAIRNGMDVQDP
ETIFIILSDMLFHPLITGFLYAALLAAVMSTISSQLLVSSSSLTEDFYRLFLRKEASDKE
CVNIGRVCVVLVGLVAAVIASDPNSQVLALVANAWAGFGAAFGPLIILSLMWPRTNGAGA
IAGMVVGAATVMIWISLGWNGSFMGGPGVYEIIPGFIAAFIAILVVSSATADAGEYQHIE
R

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory