SitesBLAST
Comparing GFF2280 PGA1_c23120 putative sorbitol dehydrogenase to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6pejA Structure of sorbitol dehydrogenase from sinorhizobium meliloti 1021 bound to sorbitol
36% identity, 97% coverage: 4:265/269 of query aligns to 2:253/257 of 6pejA
3wyeA Crystal structure of chimeric engineered (2s,3s)-butanediol dehydrogenase complexed with NAD+
38% identity, 96% coverage: 9:265/269 of query aligns to 2:251/255 of 3wyeA
- active site: G12 (= G19), S138 (= S150), Y151 (= Y163), K155 (= K167), L196 (= L208)
- binding nicotinamide-adenine-dinucleotide: G8 (= G15), Q11 (≠ R18), G12 (= G19), I13 (≠ M20), D32 (= D39), Y33 (≠ L40), V57 (≠ M65), D58 (= D66), V59 (= V67), N85 (= N93), A86 (= A94), S138 (= S150), Y151 (= Y163), K155 (= K167), P181 (= P193), G182 (= G194), V184 (= V196), T186 (= T198), M188 (≠ L200), W189 (= W201)
1gegE Cryatal structure analysis of meso-2,3-butanediol dehydrogenase (see paper)
37% identity, 96% coverage: 9:265/269 of query aligns to 3:252/256 of 1gegE
- active site: G13 (= G19), S139 (= S150), Y152 (= Y163), K156 (= K167), V197 (≠ L208)
- binding alpha-D-glucopyranose: R63 (= R70), D64 (≠ A71), F67 (≠ A74), E123 (≠ R130)
- binding nicotinamide-adenine-dinucleotide: G9 (= G15), Q12 (≠ R18), I14 (≠ M20), D33 (= D39), Y34 (≠ L40), V58 (≠ M65), D59 (= D66), V60 (= V67), N86 (= N93), A87 (= A94), I109 (≠ V116), S139 (= S150), Y152 (= Y163), K156 (= K167), P182 (= P193), V185 (= V196), T187 (= T198), M189 (≠ L200)
Q48436 Diacetyl reductase [(S)-acetoin forming]; Acetoin(diacetyl) reductase; AR; Meso-2,3-butanediol dehydrogenase; EC 1.1.1.304 from Klebsiella pneumoniae (see paper)
37% identity, 96% coverage: 9:265/269 of query aligns to 3:252/256 of Q48436
- 6:33 (vs. 12:39, 39% identical) binding
- D59 (= D66) binding
- K156 (= K167) binding
3a28C Crystal structure of l-2,3-butanediol dehydrogenase (see paper)
34% identity, 96% coverage: 9:265/269 of query aligns to 2:253/257 of 3a28C
- active site: G12 (= G19), S140 (= S150), Y153 (= Y163), K157 (= K167), L198 (= L208)
- binding nicotinamide-adenine-dinucleotide: G8 (= G15), Q11 (≠ R18), I13 (≠ M20), D32 (= D39), L33 (= L40), Q36 (≠ D43), L59 (≠ M65), D60 (= D66), V61 (= V67), N87 (= N93), S140 (= S150), Y153 (= Y163), K157 (= K167), P183 (= P193), V186 (= V196), T188 (= T198), M190 (≠ L200), W191 (= W201)
Q9ZNN8 L-2,3-butanediol dehydrogenase; L-BDH; (S,S)-butanediol dehydrogenase; Diacetyl reductase [(S)-acetoin forming]; EC 1.1.1.76; EC 1.1.1.304 from Corynebacterium glutamicum (Brevibacterium saccharolyticum) (see paper)
34% identity, 96% coverage: 9:265/269 of query aligns to 3:254/258 of Q9ZNN8
- QGI 12:14 (≠ RGM 18:20) binding
- D33 (= D39) binding
- Q37 (≠ D43) binding
- DV 61:62 (= DV 66:67) binding
- N88 (= N93) binding
- I142 (= I151) mutation to Q: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with N-148.; mutation to Q: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- F148 (≠ L157) mutation to N: Loss of L-BD oxidizing activity, and does not gain the ability to use meso-BD as substrate; when associated with Q-142.; mutation to N: Loss of L-BD oxidizing activity. Does not gain the ability to use meso-BD as substrate.
- Y154 (= Y163) binding
- K158 (= K167) binding
- PGIVGT 184:189 (≠ PGVVVT 193:198) binding
Q9KJF1 (2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA dehydrogenase subunit BbsD; (S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase subunit BbsD; EC 1.1.1.429 from Thauera aromatica (see 2 papers)
33% identity, 97% coverage: 6:265/269 of query aligns to 3:242/248 of Q9KJF1
- S15 (≠ R18) binding
- D36 (= D39) binding
- D62 (= D66) binding
- I63 (≠ V67) binding
- N89 (= N93) binding
- Y153 (= Y163) binding
- K157 (= K167) binding
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
7pcsB Structure of the heterotetrameric sdr family member bbscd (see paper)
33% identity, 97% coverage: 6:265/269 of query aligns to 2:241/247 of 7pcsB
- binding nicotinamide-adenine-dinucleotide: G11 (= G15), M16 (= M20), D35 (= D39), I36 (≠ L40), I62 (≠ V67), N88 (= N93), G90 (= G95), I138 (= I151), S140 (= S153), Y152 (= Y163), K156 (= K167), I185 (≠ V196)
5itvA Crystal structure of bacillus subtilis bacc dihydroanticapsin 7- dehydrogenase in complex with nadh (see paper)
33% identity, 97% coverage: 6:266/269 of query aligns to 5:252/255 of 5itvA
- active site: G18 (= G19), S141 (= S150), Y154 (= Y163), K158 (= K167)
- binding 1,4-dihydronicotinamide adenine dinucleotide: G14 (= G15), S17 (≠ R18), G18 (= G19), I19 (≠ M20), D38 (= D39), I39 (≠ L40), T61 (≠ M65), I63 (≠ V67), N89 (= N93), G91 (= G95), T139 (≠ V148), S141 (= S150), Y154 (= Y163), K158 (= K167), P184 (= P193), G185 (= G194), I186 (≠ V195), I187 (≠ V196)
7do7A Crystal structure of azotobacter vinelandii l-rhamnose 1- dehydrogenase(NAD and l-rhamnose bound-form) (see paper)
34% identity, 96% coverage: 9:266/269 of query aligns to 6:251/256 of 7do7A
- active site: G16 (= G19), S146 (= S150), Y159 (= Y163)
- binding nicotinamide-adenine-dinucleotide: G12 (= G15), R15 (= R18), G16 (= G19), I17 (≠ M20), S37 (≠ L40), D66 (= D66), A67 (≠ V67), N93 (= N93), A94 (= A94), G95 (= G95), I96 (≠ L96), V144 (= V148), S145 (≠ G149), S146 (= S150), Y159 (= Y163), K163 (= K167), P189 (= P193), G190 (= G194), I192 (≠ V196), T194 (= T198), I196 (≠ L200)
- binding beta-L-rhamnopyranose: F99 (≠ P99), S146 (= S150), S148 (≠ A152), Q156 (≠ V160), Y159 (= Y163), N197 (≠ W201), D235 (= D250), M236 (≠ D251), R238 (≠ D253)
7b81A Crystal structure of azotobacter vinelandii l-rhamnose 1-dehydrogenase (NAD bound-form) (see paper)
34% identity, 96% coverage: 9:266/269 of query aligns to 6:251/256 of 7b81A
- active site: G16 (= G19), S146 (= S150), Y159 (= Y163)
- binding nicotinamide-adenine-dinucleotide: G12 (= G15), S14 (≠ A17), R15 (= R18), I17 (≠ M20), D66 (= D66), A67 (≠ V67), N93 (= N93), A94 (= A94), G95 (= G95), I96 (≠ L96), T116 (≠ V116), V144 (= V148), S146 (= S150), Y159 (= Y163), K163 (= K167), P189 (= P193), G190 (= G194), I192 (≠ V196), T194 (= T198), I196 (≠ L200)
4cqlI Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD (see paper)
31% identity, 98% coverage: 4:266/269 of query aligns to 4:249/251 of 4cqlI
- active site: G19 (= G19), S146 (= S150), Y159 (= Y163), K163 (= K167)
- binding nicotinamide-adenine-dinucleotide: S18 (≠ R18), G19 (= G19), I20 (≠ M20), D39 (= D39), L40 (= L40), A64 (≠ M65), D65 (= D66), V66 (= V67), C93 (≠ N93), A94 (= A94), G95 (= G95), I96 (≠ L96), V116 (= V116), I144 (≠ V148), S146 (= S150), Y159 (= Y163), K163 (= K167), P189 (= P193), G190 (= G194), I192 (≠ V196), T194 (= T198), M196 (≠ L200)
4cqmA Crystal structure of heterotetrameric human ketoacyl reductase complexed with NAD and NADP (see paper)
31% identity, 98% coverage: 4:266/269 of query aligns to 2:246/248 of 4cqmA
- active site: G17 (= G19), S143 (= S150), Y156 (= Y163), K160 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G13 (= G15), S16 (≠ R18), G17 (= G19), I18 (≠ M20), D37 (= D39), L38 (= L40), A61 (≠ M65), V63 (= V67), C90 (≠ N93), A91 (= A94), G92 (= G95), I93 (≠ L96), V113 (= V116), I141 (≠ V148), S143 (= S150), Y156 (= Y163), K160 (= K167), P186 (= P193), G187 (= G194), I189 (≠ V196), T191 (= T198), P192 (= P199), M193 (≠ L200), T194 (≠ W201)
4za2D Crystal structure of pectobacterium carotovorum 2-keto-3-deoxy-d- gluconate dehydrogenase complexed with NAD+ (see paper)
31% identity, 97% coverage: 6:266/269 of query aligns to 8:244/247 of 4za2D
- binding nicotinamide-adenine-dinucleotide: G17 (= G15), D19 (≠ A17), L22 (≠ M20), I42 (≠ G42), D65 (= D66), M66 (≠ V67), N92 (= N93), A93 (= A94), G94 (= G95), L115 (≠ V116), I143 (≠ V148), S145 (= S150), Y158 (= Y163), K162 (= K167), G189 (= G194), M191 (≠ V196), T193 (= T198), N195 (≠ L200)
5ojiA Crystal structure of the dehydrogenase/reductase sdr family member 4 (dhrs4) from caenorhabditis elegans (see paper)
32% identity, 97% coverage: 4:265/269 of query aligns to 6:255/260 of 5ojiA
- active site: G21 (= G19), S148 (≠ G149), Y161 (= Y163), K165 (= K167)
- binding isatin: S148 (≠ G149), S150 (≠ I151), Y161 (= Y163), V193 (= V195), S199 (vs. gap), L202 (= L200)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A17 (≠ G15), T19 (≠ A17), I22 (≠ M20), S41 (≠ D39), R42 (≠ L40), N43 (≠ D41), N46 (≠ E44), I69 (≠ V67), N95 (= N93), H96 (≠ A94), G97 (= G95), N146 (= N147), S148 (≠ G149), Y161 (= Y163), K165 (= K167), G192 (= G194), I194 (≠ V196), T196 (= T198), M198 (vs. gap)
5ojgA Crystal structure of the dehydrogenase/reductase sdr family member 4 (dhrs4) from caenorhabditis elegans (see paper)
32% identity, 97% coverage: 4:265/269 of query aligns to 6:255/260 of 5ojgA
- active site: G21 (= G19), S148 (≠ G149), Y161 (= Y163), K165 (= K167)
- binding butane-2,3-dione: S148 (≠ G149), Y161 (= Y163)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: A17 (≠ G15), T19 (≠ A17), G21 (= G19), I22 (≠ M20), S41 (≠ D39), R42 (≠ L40), N43 (≠ D41), N46 (≠ E44), I69 (≠ V67), N95 (= N93), H96 (≠ A94), G97 (= G95), N146 (= N147), S148 (≠ G149), Y161 (= Y163), K165 (= K167), P191 (= P193), I194 (≠ V196), T196 (= T198), M198 (vs. gap)
Q92506 (3R)-3-hydroxyacyl-CoA dehydrogenase; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; HSD17B8; 3-ketoacyl-[acyl-carrier-protein] reductase alpha subunit; KAR alpha subunit; 3-oxoacyl-[acyl-carrier-protein] reductase; Estradiol 17-beta-dehydrogenase 8; Protein Ke6; Ke6; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17-beta-dehydrogenase 8; EC 1.1.1.n12; EC 1.1.1.62; EC 1.1.1.239 from Homo sapiens (Human) (see 2 papers)
31% identity, 98% coverage: 4:266/269 of query aligns to 7:259/261 of Q92506
- 15:23 (vs. 12:20, 56% identical) binding
- D42 (= D39) mutation to A: Reduced NADH-dependent reductase activity with acetoacetyl-CoA. Reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Increases NADPH-dependent reductase activities. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- DL 42:43 (= DL 39:40) binding
- ADV 74:76 (≠ MDV 65:67) binding
- R148 (≠ M138) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- V158 (≠ A152) to L: in a breast cancer sample; somatic mutation
- Y169 (= Y163) mutation to A: Strongly reduced NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- YAASK 169:173 (≠ YCTSK 163:167) binding
- K173 (= K167) mutation to A: Abolishes NADH-dependent reductase activity with acetoacetyl-CoA. Strongly reduced NADH-dependent reductase activity with 9,10-phenanthrene quinone. Slightly decreases NADPH-dependent reductase activity with acetoacetyl-CoA, but increases NADPH-dependent reductase activity with 9,10-phenanthrene quinone. No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- R189 (≠ E183) mutation to E: No effect on the ability to restore growth of an OAR1-deficient yeast mutant.
- IAT 202:204 (≠ VVT 196:198) binding
6zzpA Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and 3-oxovalerate (see paper)
33% identity, 97% coverage: 6:266/269 of query aligns to 8:262/265 of 6zzpA
- binding nicotinamide-adenine-dinucleotide: G17 (= G15), S20 (≠ R18), G21 (= G19), I22 (≠ M20), D41 (= D39), I42 (≠ L40), M66 (= M65), D67 (= D66), V68 (= V67), N94 (= N93), A95 (= A94), G96 (= G95), M145 (≠ V148), S147 (= S150), Y160 (= Y163), K164 (= K167), P190 (= P193), F192 (≠ V195), V193 (= V196), T195 (= T198), L197 (= L200), V198 (≠ W201)
- binding 3-oxidanylidenepentanoic acid: Q98 (≠ N97), S147 (= S150), H149 (≠ A152), K157 (≠ V160), Y160 (= Y163), F192 (≠ V195), Q201 (= Q203)
6zzoC Crystal structure of (r)-3-hydroxybutyrate dehydrogenase from psychrobacter arcticus complexed with NAD+ and acetoacetate (see paper)
33% identity, 97% coverage: 6:266/269 of query aligns to 8:262/265 of 6zzoC
- binding acetoacetic acid: Q98 (≠ N97), H149 (≠ A152), K157 (≠ V160), F192 (≠ V195), Q201 (= Q203)
- binding nicotinamide-adenine-dinucleotide: G17 (= G15), S20 (≠ R18), G21 (= G19), I22 (≠ M20), D41 (= D39), I42 (≠ L40), M66 (= M65), D67 (= D66), V68 (= V67), N94 (= N93), A95 (= A94), G96 (= G95), M145 (≠ V148), Y160 (= Y163), K164 (= K167), P190 (= P193), F192 (≠ V195), V193 (= V196), T195 (= T198), L197 (= L200), V198 (≠ W201)
4jroC Crystal structure of 3-oxoacyl-[acyl-carrier protein]reductase (fabg) from listeria monocytogenes in complex with NADP+
31% identity, 97% coverage: 6:265/269 of query aligns to 3:244/247 of 4jroC
- active site: G16 (= G19), S142 (= S150), Q152 (≠ V160), Y155 (= Y163), K159 (= K167)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G12 (= G15), S14 (≠ A17), R15 (= R18), G16 (= G19), I17 (≠ M20), N35 (≠ D39), Y36 (≠ L40), N37 (≠ D41), G38 (= G42), S39 (≠ D43), N63 (≠ D66), V64 (= V67), N90 (= N93), A91 (= A94), I93 (≠ L96), I113 (≠ V116), S142 (= S150), Y155 (= Y163), K159 (= K167), P185 (= P193), I188 (≠ V196), T190 (= T198)
Query Sequence
>GFF2280 PGA1_c23120 putative sorbitol dehydrogenase
MDPNRLKGKNILITGAARGMGQANAESFAAQGANVCLGDLDGDEAQKVADAINAAGNGMA
IAVKMDVTSRADNAAAVAATVEAFGSINVGLFNAGLNKPRFFMDIDEDNWDMIMNVNTKA
MWLGMQEVARQMIAQGPMEDHPYKLINVGSIASKKPLVDVTVYCTSKYGCLALTECGALG
LAEHNITVNGYAPGVVVTPLWEQLDKDLVDIGFKEREGQAYEDIVDSALVIKRLSYPKDI
VGTASFLASDDSDYMTGQMISIDGGWATT
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory