SitesBLAST
Comparing GFF2282 FitnessBrowser__Phaeo:GFF2282 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P35486 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; EC 1.2.4.1 from Mus musculus (Mouse) (see 2 papers)
38% identity, 97% coverage: 4:324/331 of query aligns to 54:370/390 of P35486
- S232 (≠ K184) modified: Phosphoserine; by PDK1
- S293 (≠ H246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
- S300 (≠ E253) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
- K336 (≠ E290) modified: N6-acetyllysine; mutation K->Q,R: Decreases phosphorylation at S-232 and S-300 but does not affect activity or substrate metabolism.
P26284 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; EC 1.2.4.1 from Rattus norvegicus (Rat) (see paper)
38% identity, 97% coverage: 4:324/331 of query aligns to 54:370/390 of P26284
- S232 (≠ K184) modified: Phosphoserine; by PDK1
- S293 (≠ H246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
- S300 (≠ E253) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
P29803 Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; PDHE1-A type II; EC 1.2.4.1 from Homo sapiens (Human) (see 4 papers)
37% identity, 95% coverage: 12:325/331 of query aligns to 60:369/388 of P29803
- M227 (≠ E181) to V: in SPGF70; uncertain significance; dbSNP:rs200969445
- S230 (≠ K184) mutation to A: Slightly reduces enzyme activity.
- S291 (≠ H246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4; mutation to A: Strongly reduces enzyme activity. Increases enzyme activity in stem cells.; mutation S->E,D: Abolishes enzyme activity. Increases neuronal cell death in response to glutamate excitotoxicity.
- S293 (≠ G248) modified: Phosphoserine; mutation to A: Increases enzyme activity in stem cells.; mutation to D: Abolishes enzyme activity. Increases neuronal cell death in response to glutamate excitotoxicity.
- S298 (≠ E253) modified: Phosphoserine; by PDK3; mutation to A: Slightly reduces enzyme activity.
P08559 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; EC 1.2.4.1 from Homo sapiens (Human) (see 13 papers)
38% identity, 97% coverage: 4:324/331 of query aligns to 54:370/390 of P08559
- A136 (≠ C86) to T: found in a patient with moderate developmental delay, mild dysmorphism and mildly elevated serum lactate; uncertain significance; dbSNP:rs138727886
- S232 (≠ K184) modified: Phosphoserine; by PDK1; mutation to A: Abolishes inactivation by phosphorylation; when associated with A-293 and A-300.
- M282 (= M235) to L: in dbSNP:rs2229137
- S293 (≠ H246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4; mutation to A: Reduces enzyme activity. Abolishes inactivation by phosphorylation; when associated with A-232 and A-300.; mutation to E: Interferes with substrate binding.
- S300 (≠ E253) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4; mutation to A: Abolishes inactivation by phosphorylation; when associated with A-232 and A-293.
- R302 (= R256) to C: in PDHAD; loss of activity; common mutation; dbSNP:rs137853252
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
- 10 R → P: in PDHAD; affects mitochondrial import of precursor protein; dbSNP:rs137853257
P26267 Pyruvate dehydrogenase E1 component subunit alpha type I, mitochondrial; PDHA1; PDHE1-A; EC 1.2.4.1 from Ascaris suum (Pig roundworm) (Ascaris lumbricoides) (see paper)
39% identity, 90% coverage: 9:307/331 of query aligns to 55:349/396 of P26267
- S289 (≠ H246) modified: Phosphoserine
- S296 (≠ E253) modified: Phosphoserine
6cfoA Human pyruvate dehydrogenase e1 component complex with covalent tdp adduct acetyl phosphinate (see paper)
38% identity, 97% coverage: 4:324/331 of query aligns to 26:342/362 of 6cfoA
- active site: Q52 (= Q30), G137 (≠ A117), R260 (= R241), H264 (= H245), S265 (≠ H246), Y273 (= Y255)
- binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1S)-1-hydroxy-1-[(R)-hydroxy(oxo)-lambda~5~-phosphanyl]ethyl}-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: F62 (≠ L40), Y90 (≠ H68), R91 (= R69), G137 (≠ A117), V139 (= V119), G167 (= G147), D168 (= D148), G169 (= G149), N197 (= N177), Y199 (= Y179), G200 (≠ S180), H264 (= H245)
- binding magnesium ion: D168 (= D148), N197 (= N177), Y199 (= Y179)
1ni4A Human pyruvate dehydrogenase (see paper)
38% identity, 97% coverage: 4:324/331 of query aligns to 26:342/362 of 1ni4A
- active site: Q52 (= Q30), G137 (≠ A117), R260 (= R241), H264 (= H245), S265 (≠ H246), Y273 (= Y255)
- binding magnesium ion: D168 (= D148), N197 (= N177), Y199 (= Y179)
- binding thiamine diphosphate: Y90 (≠ H68), R91 (= R69), V139 (= V119), G167 (= G147), D168 (= D148), G169 (= G149), A170 (= A150), N197 (= N177), G200 (≠ S180), H264 (= H245)
3exeA Crystal structure of the pyruvate dehydrogenase (e1p) component of human pyruvate dehydrogenase complex (see paper)
38% identity, 97% coverage: 4:324/331 of query aligns to 27:343/363 of 3exeA
- active site: Q53 (= Q30), G138 (≠ A117), R261 (= R241), H265 (= H245), S266 (≠ H246), Y274 (= Y255)
- binding manganese (ii) ion: D169 (= D148), N198 (= N177), Y200 (= Y179)
- binding thiamine diphosphate: Y91 (≠ H68), R92 (= R69), V140 (= V119), G168 (= G147), D169 (= D148), G170 (= G149), A171 (= A150), N198 (= N177), Y200 (= Y179), G201 (≠ S180), H265 (= H245)
Q8H1Y0 Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial; PDHE1-A; Protein IAA-CONJUGATE-RESISTANT 4; EC 1.2.4.1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
36% identity, 97% coverage: 4:324/331 of query aligns to 56:373/393 of Q8H1Y0
- R121 (= R69) mutation to C: In iar4-1; reduced sensitivity to several IAA-amino acid conjugates.
P16387 Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial; Pyruvate dehydrogenase complex component E1 alpha; PDHE1-A; EC 1.2.4.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
37% identity, 94% coverage: 5:314/331 of query aligns to 75:380/420 of P16387
- S313 (≠ H246) modified: Phosphoserine; by PDK1 and PDK2
Sites not aligning to the query:
- 1:33 modified: transit peptide, Mitochondrion
Q10489 Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial; PDHE1-A; EC 1.2.4.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
37% identity, 96% coverage: 10:326/331 of query aligns to 77:387/409 of Q10489
- Y306 (= Y242) modified: Phosphotyrosine
- S310 (≠ H246) modified: Phosphoserine
- S312 (≠ G248) modified: Phosphoserine
Sites not aligning to the query:
- 6 modified: Phosphothreonine
6cerA Human pyruvate dehydrogenase complex e1 component v138m mutation (see paper)
35% identity, 97% coverage: 4:324/331 of query aligns to 27:322/342 of 6cerA
6cerE Human pyruvate dehydrogenase complex e1 component v138m mutation (see paper)
35% identity, 97% coverage: 4:324/331 of query aligns to 26:320/340 of 6cerE
3exhE Crystal structure of the pyruvate dehydrogenase (e1p) component of human pyruvate dehydrogenase complex (see paper)
34% identity, 97% coverage: 4:324/331 of query aligns to 26:311/331 of 3exhE
1umdA Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 with 4-methyl-2-oxopentanoate as an intermediate (see paper)
32% identity, 93% coverage: 9:315/331 of query aligns to 31:337/362 of 1umdA
- active site: I52 (≠ Q30), S139 (≠ A117), R264 (= R241), H268 (= H245), S269 (≠ H246), Y277 (= Y255)
- binding 2-oxo-4-methylpentanoic acid: F61 (≠ L40), Y90 (≠ H68), S139 (≠ A117)
- binding magnesium ion: D170 (= D148), N199 (= N177), Y201 (= Y179)
- binding thiamine diphosphate: Y89 (≠ T67), Y90 (≠ H68), R91 (= R69), P140 (≠ I118), I141 (≠ V119), G169 (= G147), D170 (= D148), G171 (= G149), N199 (= N177), Y201 (= Y179), A202 (≠ S180), I203 (vs. gap), H268 (= H245)
1umcA Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 with 4-methylpentanoate (see paper)
32% identity, 93% coverage: 9:315/331 of query aligns to 31:337/362 of 1umcA
- active site: I52 (≠ Q30), S139 (≠ A117), R264 (= R241), H268 (= H245), S269 (≠ H246), Y277 (= Y255)
- binding 4-methyl valeric acid: Y90 (≠ H68), H126 (= H104)
- binding magnesium ion: D170 (= D148), N199 (= N177), Y201 (= Y179)
- binding thiamine diphosphate: Y89 (≠ T67), Y90 (≠ H68), R91 (= R69), I141 (≠ V119), G169 (= G147), D170 (= D148), G171 (= G149), N199 (= N177), Y201 (= Y179), I203 (vs. gap), H268 (= H245)
1umbA Branched-chain 2-oxo acid dehydrogenase (e1) from thermus thermophilus hb8 in holo-form (see paper)
32% identity, 93% coverage: 9:315/331 of query aligns to 31:337/362 of 1umbA
- active site: I52 (≠ Q30), S139 (≠ A117), R264 (= R241), H268 (= H245), S269 (≠ H246), Y277 (= Y255)
- binding magnesium ion: D170 (= D148), N199 (= N177), Y201 (= Y179)
- binding thiamine diphosphate: Y89 (≠ T67), Y90 (≠ H68), R91 (= R69), P140 (≠ I118), I141 (≠ V119), G169 (= G147), D170 (= D148), G171 (= G149), N199 (= N177), Y201 (= Y179), A202 (≠ S180), I203 (vs. gap), H268 (= H245)
Q5SLR4 2-oxoisovalerate dehydrogenase subunit alpha; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDH E1-alpha; EC 1.2.4.4 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
32% identity, 93% coverage: 9:315/331 of query aligns to 36:342/367 of Q5SLR4
3dufA Snapshots of catalysis in the e1 subunit of the pyruvate dehydrogenase multi-enzyme complex (see paper)
30% identity, 94% coverage: 2:311/331 of query aligns to 34:333/365 of 3dufA
- active site: S62 (≠ Q30), I139 (≠ A117), R264 (= R241), H268 (= H245), T269 (≠ H246), Y278 (= Y255)
- binding magnesium ion: D170 (= D148), N199 (= N177), F201 (≠ Y179)
- binding 2-{4-[(4-amino-2-methylpyrimidin-5-yl)methyl]-5-[(1r)-1-hydroxyethyl]-3-methyl-2-thienyl}ethyl trihydrogen diphosphate: Y99 (≠ H68), R100 (= R69), I141 (≠ V119), G169 (= G147), D170 (= D148), G171 (= G149), N199 (= N177), F201 (≠ Y179), A202 (≠ S180), H268 (= H245)
P11960 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; BCKDE1A; BCKDH E1-alpha; EC 1.2.4.4 from Rattus norvegicus (Rat) (see paper)
29% identity, 97% coverage: 9:330/331 of query aligns to 96:416/441 of P11960
- S333 (≠ V247) modified: Phosphoserine; by BCKDK
Query Sequence
>GFF2282 FitnessBrowser__Phaeo:GFF2282
MAKTKTNTEDYLRMYRQMVRIRSFEDNANQLYLSAKMPGLTHMYSGEEAVAVGICEALKV
TDKITSTHRGHGHCVAKGADFKEMFCELLGKEEGYCRGKGGSMHIADQSNGNLGANAIVG
GSMGIATGSAFTAKLLGKDDVTVCFFGDGATAQGLMYEVMNMAALWNLPVIYACENNGYS
EYTKTEEIAAGSITARAEAFGIEAHQVDGQDVLAVNELTQKLVARARKGEGPFFMEFMTY
RYHGHHVGDINREYYRSKEEEKDWKENRDPIIKFRGWLVEQGIASEDEIEAMNAEVKKDA
EEAVAYAEAAPYPDQSEVDMHVFTDVKHALA
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory