SitesBLAST

S232 (≠ G183) modified: Phosphoserine; by PDK1
S293 (≠ F246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
S300 (≠ T252) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
K336 (≠ E288) modified: N6-acetyllysine; mutation K->Q,R: Decreases phosphorylation at S-232 and S-300 but does not affect activity or substrate metabolism.

P26284 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; EC 1.2.4.1 from Rattus norvegicus (Rat) (see paper)
37% identity, 100% coverage: 1:325/325 of query aligns to 52:373/390 of P26284

query
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P26284

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S232 (≠ G183) modified: Phosphoserine; by PDK1
S293 (≠ F246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4
S300 (≠ T252) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4

P29803 Pyruvate dehydrogenase E1 component subunit alpha, testis-specific form, mitochondrial; PDHE1-A type II; EC 1.2.4.1 from Homo sapiens (Human) (see 4 papers)
36% identity, 99% coverage: 1:321/325 of query aligns to 50:367/388 of P29803

query
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P29803

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M227 (≠ E180) to V: in SPGF70; unknown pathological significance; dbSNP:rs200969445
S230 (≠ G183) mutation to A: Slightly reduces enzyme activity.
S291 (≠ F246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4; mutation to A: Strongly reduces enzyme activity. Increases enzyme activity in stem cells.; mutation S->E,D: Abolishes enzyme activity. Increases neuronal cell death in response to glutamate excitotoxicity.
S293 (≠ G248) modified: Phosphoserine; mutation to A: Increases enzyme activity in stem cells.; mutation to D: Abolishes enzyme activity. Increases neuronal cell death in response to glutamate excitotoxicity.
S298 (≠ T252) modified: Phosphoserine; by PDK3; mutation to A: Slightly reduces enzyme activity.

P26267 Pyruvate dehydrogenase E1 component subunit alpha type I, mitochondrial; PDHA1; PDHE1-A; EC 1.2.4.1 from Ascaris suum (Pig roundworm) (Ascaris lumbricoides) (see paper)
36% identity, 100% coverage: 1:325/325 of query aligns to 48:369/396 of P26267

query
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P26267

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S289 (≠ F246) modified: Phosphoserine
S296 (vs. gap) modified: Phosphoserine

P08559 Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial; PDHE1-A type I; EC 1.2.4.1 from Homo sapiens (Human) (see 13 papers)
37% identity, 100% coverage: 1:325/325 of query aligns to 52:373/390 of P08559

query
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P08559

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A136 (= A85) to T: probable disease-associated variant found in a patient with moderate developmental delay, mild dysmorphism and mildly elevated serum lactate; dbSNP:rs138727886
S232 (≠ G183) modified: Phosphoserine; by PDK1; mutation to A: Abolishes inactivation by phosphorylation; when associated with A-293 and A-300.
M282 (≠ V235) to L: in dbSNP:rs2229137
S293 (≠ F246) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4; mutation to A: Reduces enzyme activity. Abolishes inactivation by phosphorylation; when associated with A-232 and A-300.; mutation to E: Interferes with substrate binding.
S300 (≠ T252) modified: Phosphoserine; by PDK1, PDK2, PDK3 and PDK4; mutation to A: Abolishes inactivation by phosphorylation; when associated with A-232 and A-293.
R302 (= R254) to C: in PDHAD; loss of activity; common mutation; dbSNP:rs137853252

Sites not aligning to the query:

1:29 modified: transit peptide, Mitochondrion
10 R → P: in PDHAD; affects mitochondrial import of precursor protein; dbSNP:rs137853257

6cfoA Human pyruvate dehydrogenase e1 component complex with covalent tdp adduct acetyl phosphinate (see paper)
37% identity, 100% coverage: 1:325/325 of query aligns to 24:345/362 of 6cfoA

query
sites
6cfoA

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active site: Q52 (≠ V29), G137 (= G116), R260 (= R241), H264 (= H245), S265 (≠ F246), Y273 (= Y253)
binding 3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-{(1S)-1-hydroxy-1-[(R)-hydroxy(oxo)-lambda~5~-phosphanyl]ethyl}-5-(2-{[(S)-hydroxy(phosphonooxy)phosphoryl]oxy}ethyl)-4-methyl-1,3-thiazol-3-ium: F62 (= F39), Y90 (≠ H67), R91 (= R68), G137 (= G116), V139 (= V118), G167 (= G146), D168 (= D147), G169 (= G148), N197 (= N176), Y199 (= Y178), G200 (≠ A179), H264 (= H245)
binding magnesium ion: D168 (= D147), N197 (= N176), Y199 (= Y178)

1ni4A Human pyruvate dehydrogenase (see paper)
37% identity, 100% coverage: 1:325/325 of query aligns to 24:345/362 of 1ni4A

query
sites
1ni4A

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active site: Q52 (≠ V29), G137 (= G116), R260 (= R241), H264 (= H245), S265 (≠ F246), Y273 (= Y253)
binding magnesium ion: D168 (= D147), N197 (= N176), Y199 (= Y178)
binding thiamine diphosphate: Y90 (≠ H67), R91 (= R68), V139 (= V118), G167 (= G146), D168 (= D147), G169 (= G148), A170 (≠ G149), N197 (= N176), G200 (≠ A179), H264 (= H245)

3exeA Crystal structure of the pyruvate dehydrogenase (e1p) component of human pyruvate dehydrogenase complex (see paper)
37% identity, 100% coverage: 1:325/325 of query aligns to 25:346/363 of 3exeA

query
sites
3exeA

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active site: Q53 (≠ V29), G138 (= G116), R261 (= R241), H265 (= H245), S266 (≠ F246), Y274 (= Y253)
binding manganese (ii) ion: D169 (= D147), N198 (= N176), Y200 (= Y178)
binding thiamine diphosphate: Y91 (≠ H67), R92 (= R68), V140 (= V118), G168 (= G146), D169 (= D147), G170 (= G148), A171 (≠ G149), N198 (= N176), Y200 (= Y178), G201 (≠ A179), H265 (= H245)

P16387 Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial; Pyruvate dehydrogenase complex component E1 alpha; PDHE1-A; EC 1.2.4.1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 2 papers)
36% identity, 99% coverage: 1:322/325 of query aligns to 72:392/420 of P16387

query
sites
P16387

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D

-

Q

Y

E

A

K

P

G

G

M

F

L

Y

G

G

A

G

N

N

G

G

I

I

V

V

G

G

A

A

G

Q

A

V

P

P

L

L

A

G

A

A

G

G

A

L

A

A

L

F

A

A

S

H

K

Q

L

Y

K

K

G

N

S

E

Q

D

G

A

V

C

A

S

V

F

A

T

F

L

F

Y

G

G

D

D

G

G

G

A

S

S

N

N

E

Q

G

G

A

Q

V

V

F

F

E

E

A

S

M

F

N

N

L

M

A

A

S

K

I

L

M

W

K

N

L

L

P

P

C

V

L

V

F

F

V

C

A

C

E

E

N

N

G

K

Y

Y

A

G

E

M

A

G

T

T

G

A

S

A

G

S

W

R

S

S

V

S

A

A

C

M

K

T

D

E

I

Y

A

F

E

K

R

R

A

-

V

-

G

G

F

Q

G

Y

M

I

P

P

G

G

V

L

I

K

V

V

D

N

G

G

N

M

D

D

F

I

F

L

A

A

V

V

H

Y

A

Q

A

A

L

S

G

K

V

F

A

A

V

K

E

D

R

W

A

C

R

L

K

S

G

G

D

K

G

G

P

P

T

L

L

V

V

L

E

E

V

Y

K

E

L

T

S

Y

R

R

F

Y

Y

G

G

G

H

H

F
x
S

E

M

G

S

D

D

A

P

-

G

Q

T

T

T

Y

Y

R

R

G

T

P

R

D

D

E

E

V

I

K

Q

N

H

L

M

R

R

E

S

N

K

A

N

D

D

C

P

L

I

A

A

L

G

F

L

R

K

Q

M

R

H

C

L

S

I

A

D

E

L

G

G

W

I

L

A

D

T

A

E

A

A

Q

E

F

V

E

K

R

A

I

Y

D

D

G

K

E

S

V

A

A

R

Q

K

L

Y

I

V

E

D

D

E

A

Q

V

V

R

E

L

L

A

A

K

D

S

A

D

A

P

P

K

P

P

P

Q

E

A

A

-

K

-

L

A

S

D

I

L

L

L

F

S

E

D

D

V

V

Y

Y

V

V

S313 (≠ F246) modified: Phosphoserine; by PDK1 and PDK2

Sites not aligning to the query:

1:33 modified: transit peptide, Mitochondrion

Q8H1Y0 Pyruvate dehydrogenase E1 component subunit alpha-2, mitochondrial; PDHE1-A; Protein IAA-CONJUGATE-RESISTANT 4; EC 1.2.4.1 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 99% coverage: 2:322/325 of query aligns to 55:373/393 of Q8H1Y0

query
sites
Q8H1Y0

S

S

T

V

H

E

L

T

S

S

T

S

D

E

Q

E

L

I

L

L

H

A

A

F

Y

F

T

R

V

D

M

M

R

A

T

R

I

M

R

R

D

R

F

M

E

E

E

I

R

A

L

A

H

D

V

S

E

L

F

Y

A

K

T

A

G

K

E

L

I

I

P

R

G

G

F

F

V

C

H

H

L

L

Y

Y

A

D

G

G

Q

Q

E

E

A

A

S

L

A

A

A

V

G

G

V

M

M

E

A

A

H

A

L

I

N

T

D

K

E

K

D

D

C

A

I

I

A

I

S

T

N

S

H

Y

R
|
R

G

D

H

H

G

C

H

T

C

F

I

I

A

G

K

R

G

G

V

G

D

K

V

L

F

V

G

D

M

A

M

F

A

S

E

E

I

L

Y

M

G

G

K

R

K

K

T

T

G

G

V

C

C

S

G

H

G

G

K

K

G

G

S

S

M

M

H

H

I

F

A

Y

D

K

Q

K

E

D

K

A

G

S

M

F

L

Y

G

G

A

G

N

H

G

G

I

I

V

V

G

G

A

A

G

Q

A

I

P

P

L

L

A

G

A

C

G

G

A

L

A

A

L

F

A

A

S

Q

K

K

L

Y

K

N

G

K

S

D

Q

E

G

A

V

V

A

T

V

F

A

A

F

L

F

Y

G

G

D

D

G

G

G

A

S

A

N

N

E

Q

G

G

A

Q

V

L

F

F

E

E

A

A

M

L

N

N

L

I

A

S

S

A

I

L

M

W

K

D

L

L

P

P

C

A

L

I

F

L

V

V

A

C

E

E

N

N

G

H

Y

Y

A

G

E

M

A

G

T

T

G

A

S

T

G

-

W

W

S

R

V

S

A

A

C

-

K

K

D

S

I

P

A

A

E

Y

R

F

A

K

V

R

G

G

F

D

G

Y

M

V

P

P

G

G

V

L

I

K

V

V

D

D

G

G

N

M

D

D

F

A

F

L

A

A

V

V

H

K

A

Q

A

A

L

C

G

K

V

F

A

A

V

K

E

E

R

H

A

A

R

L

K

K

G

-

D

N

G

G

P

P

T

I

L

I

V

L

E

E

V

M

K

D

L

T

S

Y

R

R

F

Y

Y

H

G

G

H

H

F

S

E

M

G

S

D

D

A

P

-

G

Q

S

T

T

Y

Y

R

R

G

T

P

R

D

D

E

E

V

I

K

S

N

G

L

V

R

R

E

Q

N

V

A

R

D

D

C

P

L

I

A

E

L

R

F

V

R

R

Q

K

R

L

C

L

S

L

A

T

E

H

G

D

W

I

L

A

D

T

A

E

A

K

Q

E

F

L

E

K

R

D

I

M

D

E

G

K

E

E

V

I

A

R

Q

K

L

E

I

V

E

D

D

D

A

A

V

V

R

A

L

Q

A

A

K

K

S

E

D

S

P

P

K

I

P

P

Q

D

A

A

A

S

D

E

L

L

L

F

S

T

D

N

V

M

Y

Y

V

V

R121 (= R68) mutation to C: In iar4-1; reduced sensitivity to several IAA-amino acid conjugates.

Q10489 Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial; PDHE1-A; EC 1.2.4.1 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
35% identity, 98% coverage: 4:323/325 of query aligns to 72:390/409 of Q10489

query
sites
Q10489

H

E

L

V

S

T

T

K

D

G

Q

E

L

L

H

G

A

L

Y

Y

T

E

V

K

M

M

R

V

T

T

I

I

R

R

D

R

F

L

E

E

E

L

R

A

L

C

H

D

V

A

E

L

F

Y

A

K

T

A

G

K

E

K

I

I

P

R

G

G

F

F

V

C

H

H

L

L

Y

S

A

I

G

G

Q

Q

E

E

A

A

S

V

A

A

G

G

V

I

M

E

A

G

H

A

L

I

N

T

D

L

E

D

D

D

C

S

I

I

A

I

S

T

N

S

H

Y

R

R

G

C

H

H

G

G

H

F

C

A

I

Y

A

T

K

R

G

G

V

L

D

S

V

I

F

R

G

S

M

I

A

G

E

E

I

L

Y

M

G

G

K

R

K

Q

T

C

G

G

V

A

C

S

G

K

G

G

K

K

G

G

S

S

M

M

H

H

I

I

A

F

D

-

Q

-

E

A

K

K

G

N

M

F

L

Y

G

G

A

G

N

N

G

G

I

I

V

V

G

G

A

A

G

Q

A

I

P

P

L

L

A

G

A

A

G

G

A

I

A

G

L

F

A

A

S

Q

K

K

L

Y

K

L

G

E

S

K

Q

P

G

T

V

T

A

T

V

F

A

A

F

L

F

Y

G

G

D

D

G

G

G

A

S

S

N

N

E

Q

G

G

A

Q

V

A

F

F

E

E

A

A

M

F

N

N

L

M

A

A

S

K

I

L

M

W

K

G

L

L

P

P

C

V

L

I

F

F

V

A

A

C

E

E

N

N

G

K

Y

Y

A

G

E

M

A

G

T

T

G

S

S

A

G

E

W

R

S

S

V

S

A

A

C

M

K

T

D

E

I

F

A

Y

E

K

R

R

A

-

V

-

G

G

F

Q

G

Y

M

I

P

P

G

G

V

L

I

L

V

V

D

N

G

G

N

M

D

D

F

V

F

L

A

A

V

V

H

L

A

Q

A

A

L

S

G

K

V

F

A

A

V

K

E

K

R

Y

A

T

R

V

K

E

G

N

D

S

G

Q

P

P

T

L

L

L

V

M

E

E

V

F

K

V

L

T

S

Y

R

R

F
x
Y

Y

G

G

G

H

H

F
x
S

E

M

G
x
S

D

D

A

P

-

G

Q

T

T

T

Y

Y

R

R

G

S

P

R

D

E

E

E

V

V

K

Q

N

K

L

V

R

R

E

A

N

A

A

R

D

D

C

P

L

I

A

E

L

G

F

L

R

K

Q

K

R

H

C

I

S

M

A

E

E

W

G

G

W

V

L

A

D

N

A

A

A

N

Q

E

F

L

E

K

R

N

I

I

D

E

G

K

E

R

V

I

A

R

Q

G

L

M

I

V

E

D

D

E

A

E

V

V

R

R

L

I

A

A

K

E

S

E

D

S

P

P

K

F

P

P

Q

D

A

P

A

I

D

E

-

E

-

S

L

L

L

F

S

S

D

D

V

V

Y

Y

V

V

A

A

Y306 (≠ F242) modified: Phosphotyrosine
S310 (≠ F246) modified: Phosphoserine
S312 (≠ G248) modified: Phosphoserine

Sites not aligning to the query:

6 modified: Phosphothreonine

6cerE Human pyruvate dehydrogenase complex e1 component v138m mutation (see paper)
36% identity, 100% coverage: 1:325/325 of query aligns to 24:323/340 of 6cerE

query
sites
6cerE

M

V

S

T

T

T

H

V

L

L

S

T

T

R

D

E

Q

D

L

G

L

L

H

K

A

Y

Y

Y

T

R

V

M

M

M

R

Q

T

T

I

V

R

R

D

R

F

M

E

E

E

L

R

K

L

A

H

D

V
x
Q

E

L

F

Y

A

K

T

Q

G

K

E

I

I

I

P

R

G

G

F

F

V

C

H

H

L

L

Y

C

A

D

G

G

Q

Q

E

E

A

A

S

C

A

C

A

V

G

G

V

L

M

E

A

A

H

G

L

I

N

N

D

P

E

T

D

D

C

H

I

L

A

I

S

T

N

A

H
x
Y

R
|
R

G

A

H

H

G

G

H

F

C

T

I

F

A

T

K

R

G

G

V

L

D

S

V

V

F

R

G

E

M

I

M

L

A

A

E

E

I

L

Y

T

G

G

K

R

K

K

T

G

G

G

V

C

C

A

G

K

G

G

K

K

G

G

S

S

M

M

H

H

I

M

A

-

D

-

Q

Y

E

A

K

K

G

N

M

F

L

Y

G

G

A

G

N

N

G
|
G

I

I

V
x
M

G

G

A

A

G

Q

A

V

P

P

L

L

A

G

A

A

G

G

A

I

A

A

L

L

A

A

S

C

K

K

L

Y

K

N

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G

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K

Q

D

G

E

V

V

A

C

V

L

A

T

F

L

F

Y

G

G

D

D

G

G

G

A

S

A

N

N

E

Q

G

G

A

Q

V

I

F

F

E

E

A

A

M

Y

N

N

L

M

A

A

S

A

I

L

M

W

K

K

L

L

P

P

C

C

L

I

F

F

V

I

A

C

E

E

N

N

G

R

Y

R

A

A

E

A

A

A

T

S

G

-

S

-

G

-

W

-

S

-

V

-

A

-

C

-

K

T

D

D

I

Y

A

Y

E

K

R

R

A

-

V

-

G

G

F

D

G

F

M

I

P

P

G

G

V

L

I

R

V

V

D

D

G

G

N

M

D

D

F

I

F

L

A

C

V

V

H

R

A

E

A

A

L

T

G

R

V

F

A

A

V

A

E

A

R

Y

A

C

R

R

K

S

G

G

D

K

G

G

P

P

T

I

L

L

V

M

E

E

V

L

K

-

L

-

S

-

R

-

F

-

Y

-

G

-

H

-

F

-

E

-

G

-

D

-

A

-

Q

Q

T

T

Y

Y

R
|
R

G

Y

P
x
H

D

E

E

E

V

I

K

Q

N

E

L

V

R

R

E

S

N

K

A

S

D

D

C

P

L

I

A

M

L

L

F

L

R

K

Q

D

R

R

C

M

S

V

A

N

E

S

G

N

W

L

L

A

D

S

A

V

A

E

Q

E

F

L

E

K

R

E

I

I

D

D

G

V

E

E

V

V

A

R

Q

K

L

E

I

I

E

E

D

D

A

A

V

A

R

Q

L

F

A

A

K

T

S

A

D

D

P

P

K

E

P

P

Q

P

A

L

A

E

D

E

L

L

L

G

S

Y

D

H

V

I

Y

Y

V

S

A

S

Y

D

P

P

active site: Q52 (≠ V29), G137 (= G116), R252 (= R254)
binding magnesium ion: R252 (= R254), H254 (≠ P256)
binding thiamine diphosphate: Y90 (≠ H67), R91 (= R68), M139 (≠ V118), R252 (= R254)

6cerA Human pyruvate dehydrogenase complex e1 component v138m mutation (see paper)
35% identity, 100% coverage: 1:325/325 of query aligns to 25:325/342 of 6cerA

query
sites
6cerA

M

V

S

T

T

T

H

V

L

L

S

T

T

R

D

E

Q

D

L

G

L

L

H

K

A

Y

Y

Y

T

R

V

M

M

M

R

Q

T

T

I

V

R

R

D

R

F

M

E

E

E

L

R

K

L

A

H

D

V
x
Q

E

L

F

Y

A

K

T

Q

G

K

E

I

I

I

P

R

G

G

F

F

V

C

H

H

L

L

Y

C

A

D

G

G

Q

Q

E

E

A

A

S

C

A

C

A

V

G

G

V

L

M

E

A

A

H

G

L

I

N

N

D

P

E

T

D

D

C

H

I

L

A

I

S

T

N

A

H
x
Y

R
|
R

G

A

H

H

G

G

H

F

C

T

I

F

A

T

K

R

G

G

V

L

D

S

V

V

F

R

G

E

M

I

M

L

A

A

E

E

I

L

Y

T

G

G

K

R

K

K

T

G

G

G

V

C

C

A

G

K

G

G

K

K

G

G

S

S

M

M

H

H

I

M

A

-

D

-

Q

Y

E

A

K

K

G

N

M

F

L

Y

G

G

A

G

N

N

G
|
G

I

I

V
x
M

G

G

A

A

G

Q

A

V

P

P

L

L

A

G

A

A

G

G

A

I

A

A

L

L

A

A

S

C

K

K

L

Y

K

N

G

G

S

K

Q

D

G

E

V

V

A

C

V

L

A

T

F

L

F

Y

G

G

D

D

G

G

G

A

S

A

N

N

E

Q

G

G

A

Q

V

I

F

F

E

E

A

A

M

Y

N

N

L

M

A

A

S

A

I

L

M

W

K

K

L

L

P

P

C

C

L

I

F

F

V

I

A

C

E

E

N

N

G

R

Y

A

A

A

E

A

A

S

T

T

G

-

S

-

G

-

W

-

S

-

V

-

A

-

C

-

K

-

D

D

I

Y

A

Y

E

K

R

R

A

-

V

-

G

G

F

D

G

F

M

I

P

P

G

G

V

L

I

R

V

V

D

D

G

G

N

M

D

D

F

I

F

L

A

C

V

V

H

R

A

E

A

A

L

T

G

R

V

F

A

A

V

A

E

A

R

Y

A

C

R

R

K

S

G

G

D

K

G

G

P

P

T

I

L

L

V

M

E

E

V

L

K

Q

L

T

S

Y

R
|
R

F

Y

Y

H

G

T

H

R

F

-

E

-

G

-

D

-

A

-

Q

-

T

-

Y

-

R

-

G

-

P

-

D

E

E

E

V

I

K

Q

N

E

L

V

R

R

E

S

N

K

A

S

D

D

C

P

L

I

A

M

L

L

F

L

R

K

Q

D

R

R

C

M

S

V

A

N

E

S

G

N

W

L

L

A

D

S

A

V

A

E

Q

E

F

L

E

K

R

E

I

I

D

D

G

V

E

E

V

V

A

R

Q

K

L

E

I

I

E

E

D

D

A

A

V

A

R

Q

L

F

A

A

K

T

S

A

D

D

P

P

K

E

P

P

Q

P

A

L

A

E

D

E

L

L

L

G

S

Y

D

H

V

I

Y

Y

V

S

A

S

Y

D

P

P

active site: Q53 (≠ V29), G138 (= G116), R252 (= R241)
binding thiamine diphosphate: Y91 (≠ H67), R92 (= R68), M140 (≠ V118), R252 (= R241)

3exhE Crystal structure of the pyruvate dehydrogenase (e1p) component of human pyruvate dehydrogenase complex (see paper)
35% identity, 100% coverage: 1:325/325 of query aligns to 24:314/331 of 3exhE

query
sites
3exhE

M

V

S

T

T

T

H

V

L

L

S

T

T

R

D

E

Q

D

L

G

L

L

H

K

A

Y

Y

Y

T

R

V

M

M

M

R

Q

T

T

I

V

R

R

D

R

F

M

E

E

E

L

R

K

L

A

H

D

V
x
Q

E

L

F

Y

A

K

T

Q

G

K

E

I

I

I

P

R

G

G

F

F

V

C

H

H

L

L

Y

C

A

D

G

G

Q

Q

E

E

A

A

S

C

A

C

A

V

G

G

V

L

M

E

A

A

H

G

L

I

N

N

D

P

E

T

D

D

C

H

I

L

A

I

S

T

N

A

H
x
Y

R
|
R

G

A

H

H

G

G

H

F

C

T

I

F

A

T

K

R

G

G

V

L

D

S

V

V

F

R

G

E

M

I

M

L

A

A

E

E

I

L

Y

T

G

G

K

R

K

K

T

G

G

G

V

C

C

A

G

K

G

G

K

K

G

G

S

S

M

M

H

H

I

M

A

-

D

-

Q

Y

E

A

K

K

G

N

M

F

L

Y

G

G

A

G

N

N

G
|
G

I

I

V
|
V

G

G

A

A

G

Q

A

V

P

P

L

L

A

G

A

A

G

G

A

I

A

A

L

L

A

A

S

C

K

K

L

Y

K

N

G

G

S

K

Q

D

G

E

V

V

A

C

V

L

A

T

F

L

F

Y

G
|
G

D
|
D

G
|
G

G

A

S

A

N

N

E

Q

G

G

A

Q

V

I

F

F

E

E

A

A

M

Y

N

N

L

M

A

A

S

A

I

L

M

W

K

K

L

L

P

P

C

C

L

I

F

F

V

I

A

C

E

E

N

N

N
|
N

G

R

Y

E

A

R

E

A

A

A

T

-

G

-

S

-

G

-

W

-

S

-

V

-

A

A

C

S

K

T

D

D

I

Y

A

Y

E

K

R

R

A

-

V

-

G

G

F

D

G

F

M

I

P

P

G

G

V

L

I

R

V

V

D

D

G

G

N

M

D

D

F

I

F

L

A

C

V

V

H

R

A

E

A

A

L

T

G

R

V

F

A

A

V

A

E

A

R

Y

A

C

R

R

K

S

G

G

D

K

G

G

P

P

T

I

L

L

V

M

E

E

V

L

K

-

L

-

S

-

R

-

F

-

Y

-

G

-

H

-

F

-

E

-

G

-

D

-

A

-

Q

Q

T

T

Y

Y

R

-

G

-

P

-

D

-

E

-

V

-

K

-

N

-

L

-

R

-

E

S

N

K

A

S

D

D

C

P

L

I

A

M

L

L

F

L

R

K

Q

D

R

R

C

M

S

V

A

N

E

S

G

N

W

L

L

A

D

S

A

V

A

E

Q

E

F

L

E

K

R

E

I

I

D

D

G

V

E

E

V

V

A

R

Q

K

L

E

I

I

E

E

D

D

A

A

V

A

R

Q

L

F

A

A

K

T

S

A

D

D

P

P

K

E

P

P

Q

P

A

L

A

E

D

E

L

L

L

G

S

Y

D

H

V

I

Y

Y

V

S

A

S

Y

D

P

P

active site: Q52 (≠ V29), G137 (= G116)
binding manganese (ii) ion: